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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 832 |
| Acc16I | TGCGCA | 1 cut(s) | 83 |
| AccII | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| AccIII | TCCGGA | 1 cut(s) | 262 |
| AciI | CCGC | 9 cut(s) | 56, 336, 449, 465, 540, 542, 776, 810, 840 |
| AclWI | GGATC | 2 cut(s) | 647, 660 |
| AcoI | YGGCCR | 2 cut(s) | 594, 679 |
| AcuI | CTGAAG | 1 cut(s) | 800 |
| AfaI | GTAC | 2 cut(s) | 694, 704 |
| AfeI | AGCGCT | 1 cut(s) | 798 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 220, 285 |
| AflII | CTTAAG | 1 cut(s) | 152 |
| AgsI | TTSAA | 6 cut(s) | 31, 314, 347, 616, 637, 874 |
| AluBI | AGCT | 8 cut(s) | 6, 13, 21, 61, 135, 175, 644, 758 |
| AluI | AGCT | 8 cut(s) | 6, 13, 21, 61, 135, 175, 644, 758 |
| Alw21I | GWGCWC | 1 cut(s) | 8 |
| AlwI | GGATC | 2 cut(s) | 647, 660 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 761 |
| Aor13HI | TCCGGA | 1 cut(s) | 262 |
| Aor51HI | AGCGCT | 1 cut(s) | 798 |
| AoxI | GGCC | 5 cut(s) | 456, 594, 679, 846, 867 |
| ApaI | GGGCCC | 1 cut(s) | 460 |
| ApeKI | GCWGC | 8 cut(s) | 21, 58, 70, 89, 709, 735, 743, 813 |
| AspLEI | GCGC | 3 cut(s) | 84, 449, 799 |
| AspS9I | GGNCC | 2 cut(s) | 456, 457 |
| AsuHPI | GGTGA | 1 cut(s) | 45 |
| AsuNHI | GCTAGC | 1 cut(s) | 476 |
| BaeGI | GKGCMC | 1 cut(s) | 460 |
| BalI | TGGCCA | 2 cut(s) | 596, 681 |
| BamHI | GGATCC | 1 cut(s) | 652 |
| BanII | GRGCYC | 2 cut(s) | 8, 460 |
| BbsI | GAAGAC | 2 cut(s) | 551, 734 |
| Bbv12I | GWGCWC | 1 cut(s) | 8 |
| BbvCI | CCTCAGC | 1 cut(s) | 754 |
| BbvI | GCAGC | 8 cut(s) | 8, 57, 70, 76, 696, 730, 747, 825 |
| BccI | CCATC | 3 cut(s) | 327, 563, 694 |
| BciVI | GTATCC | 1 cut(s) | 409 |
| BfaI | CTAG | 5 cut(s) | 18, 386, 420, 477, 732 |
| BfmI | CTRYAG | 1 cut(s) | 71 |
| BfoI | RGCGCY | 1 cut(s) | 800 |
| BfrI | CTTAAG | 1 cut(s) | 152 |
| BfuI | GTATCC | 1 cut(s) | 409 |
| BmgT120I | GGNCC | 2 cut(s) | 456, 457 |
| BmiI | GGNNCC | 3 cut(s) | 211, 458, 654 |
| BmsI | GCATC | 2 cut(s) | 868, 871 |
| BmtI | GCTAGC | 1 cut(s) | 480 |
| BpiI | GAAGAC | 2 cut(s) | 551, 734 |
| Bpu10I | CCTNAGC | 1 cut(s) | 754 |
| BpuEI | CTTGAG | 1 cut(s) | 111 |
| BsaAI | YACGTR | 1 cut(s) | 706 |
| BsaJI | CCNNGG | 4 cut(s) | 279, 540, 588, 667 |
| BsaWI | WCCGGW | 2 cut(s) | 262, 689 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 220, 285 |
| Bse3DI | GCAATG | 1 cut(s) | 745 |
| BseAI | TCCGGA | 1 cut(s) | 262 |
| BseDI | CCNNGG | 4 cut(s) | 279, 540, 588, 667 |
| BseGI | GGATG | 3 cut(s) | 187, 574, 686 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 220, 285 |
| BseMI | GCAATG | 1 cut(s) | 745 |
| BseMII | CTCAG | 1 cut(s) | 768 |
| BseSI | GKGCMC | 1 cut(s) | 460 |
| BseXI | GCAGC | 8 cut(s) | 8, 57, 70, 76, 696, 730, 747, 825 |
| Bsh1236I | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| BshFI | GGCC | 5 cut(s) | 458, 596, 681, 848, 869 |
| BsiHKAI | GWGCWC | 1 cut(s) | 8 |
| BsiSI | CCGG | 2 cut(s) | 263, 690 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 220, 285 |
| BsnI | GGCC | 5 cut(s) | 458, 596, 681, 848, 869 |
| Bsp120I | GGGCCC | 1 cut(s) | 456 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 460 |
| Bsp13I | TCCGGA | 1 cut(s) | 262 |
| Bsp143I | GATC | 4 cut(s) | 354, 388, 429, 652 |
| BspACI | CCGC | 9 cut(s) | 56, 336, 449, 465, 540, 542, 776, 810, 840 |
| BspANI | GGCC | 5 cut(s) | 458, 596, 681, 848, 869 |
| BspCNI | CTCAG | 1 cut(s) | 767 |
| BspEI | TCCGGA | 1 cut(s) | 262 |
| BspFNI | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| BspLI | GGNNCC | 3 cut(s) | 211, 458, 654 |
| BspMAI | CTGCAG | 1 cut(s) | 75 |
| BspOI | GCTAGC | 1 cut(s) | 480 |
| BspPI | GGATC | 2 cut(s) | 647, 660 |
| BspTI | CTTAAG | 1 cut(s) | 152 |
| BsrDI | GCAATG | 1 cut(s) | 745 |
| BssECI | CCNNGG | 4 cut(s) | 279, 540, 588, 667 |
| BssMI | GATC | 4 cut(s) | 354, 388, 429, 652 |
| BssT1I | CCWWGG | 1 cut(s) | 667 |
| Bst4CI | ACNGT | 2 cut(s) | 220, 763 |
| Bst6I | CTCTTC | 1 cut(s) | 776 |
| BstAFI | CTTAAG | 1 cut(s) | 152 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 79 |
| BstBAI | YACGTR | 1 cut(s) | 706 |
| BstC8I | GCNNGC | 2 cut(s) | 478, 795 |
| BstDEI | CTNAG | 1 cut(s) | 754 |
| BstDSI | CCRYGG | 1 cut(s) | 540 |
| BstF5I | GGATG | 3 cut(s) | 187, 574, 686 |
| BstFNI | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| BstH2I | RGCGCY | 1 cut(s) | 800 |
| BstHHI | GCGC | 3 cut(s) | 84, 449, 799 |
| BstKTI | GATC | 4 cut(s) | 357, 391, 432, 655 |
| BstMBI | GATC | 4 cut(s) | 354, 388, 429, 652 |
| BstNSI | RCATGY | 1 cut(s) | 410 |
| BstSFI | CTRYAG | 1 cut(s) | 71 |
| BstSLI | GKGCMC | 1 cut(s) | 460 |
| BstUI | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| BstV1I | GCAGC | 8 cut(s) | 8, 57, 70, 76, 696, 730, 747, 825 |
| BstV2I | GAAGAC | 2 cut(s) | 551, 734 |
| BstX2I | RGATCY | 1 cut(s) | 652 |
| BstYI | RGATCY | 1 cut(s) | 652 |
| BsuI | GTATCC | 1 cut(s) | 409 |
| BsuRI | GGCC | 5 cut(s) | 458, 596, 681, 848, 869 |
| BtgI | CCRYGG | 1 cut(s) | 540 |
| BtgZI | GCGATG | 1 cut(s) | 305 |
| BtsCI | GGATG | 3 cut(s) | 187, 574, 686 |
| BtsIMutI | CAGTG | 2 cut(s) | 225, 497 |
| Cac8I | GCNNGC | 2 cut(s) | 478, 795 |
| CaiI | CAGNNNCTG | 1 cut(s) | 761 |
| CfoI | GCGC | 3 cut(s) | 84, 449, 799 |
| Cfr13I | GGNCC | 2 cut(s) | 456, 457 |
| Cfr42I | CCGCGG | 1 cut(s) | 543 |
| Csp6I | GTAC | 2 cut(s) | 693, 703 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 573, 608 |
| CviAII | CATG | 9 cut(s) | 85, 301, 407, 461, 508, 550, 598, 722, 882 |
| CviQI | GTAC | 2 cut(s) | 693, 703 |
| DdeI | CTNAG | 1 cut(s) | 754 |
| DpnI | GATC | 4 cut(s) | 356, 390, 431, 654 |
| DpnII | GATC | 4 cut(s) | 354, 388, 429, 652 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 832 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 832 |
| EaeI | YGGCCR | 2 cut(s) | 594, 679 |
| Eam1104I | CTCTTC | 1 cut(s) | 776 |
| EarI | CTCTTC | 1 cut(s) | 776 |
| Ecl136II | GAGCTC | 1 cut(s) | 6 |
| Eco130I | CCWWGG | 1 cut(s) | 667 |
| Eco147I | AGGCCT | 1 cut(s) | 869 |
| Eco24I | GRGCYC | 2 cut(s) | 8, 460 |
| Eco32I | GATATC | 1 cut(s) | 142 |
| Eco47III | AGCGCT | 1 cut(s) | 798 |
| Eco53kI | GAGCTC | 1 cut(s) | 6 |
| Eco57I | CTGAAG | 1 cut(s) | 800 |
| EcoICRI | GAGCTC | 1 cut(s) | 6 |
| EcoO109I | RGGNCCY | 1 cut(s) | 456 |
| EcoRV | GATATC | 1 cut(s) | 142 |
| EcoT14I | CCWWGG | 1 cut(s) | 667 |
| EcoT22I | ATGCAT | 1 cut(s) | 883 |
| EcoT38I | GRGCYC | 2 cut(s) | 8, 460 |
| ErhI | CCWWGG | 1 cut(s) | 667 |
| FaeI | CATG | 9 cut(s) | 88, 304, 410, 464, 511, 553, 601, 725, 885 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 226, 258 |
| FatI | CATG | 9 cut(s) | 84, 300, 406, 460, 507, 549, 597, 721, 881 |
| FokI | GGATG | 3 cut(s) | 194, 581, 673 |
| FriOI | GRGCYC | 2 cut(s) | 8, 460 |
| FspBI | CTAG | 5 cut(s) | 18, 386, 420, 477, 732 |
| FspI | TGCGCA | 1 cut(s) | 83 |
| GlaI | GCGC | 3 cut(s) | 83, 448, 798 |
| HaeII | RGCGCY | 1 cut(s) | 800 |
| HaeIII | GGCC | 5 cut(s) | 458, 596, 681, 848, 869 |
| HapII | CCGG | 2 cut(s) | 263, 690 |
| HhaI | GCGC | 3 cut(s) | 84, 449, 799 |
| Hin1II | CATG | 9 cut(s) | 88, 304, 410, 464, 511, 553, 601, 725, 885 |
| Hin6I | GCGC | 3 cut(s) | 82, 447, 797 |
| HinP1I | GCGC | 3 cut(s) | 82, 447, 797 |
| HindIII | AAGCTT | 1 cut(s) | 642 |
| HinfI | GANTC | 2 cut(s) | 116, 487 |
| HpaII | CCGG | 2 cut(s) | 263, 690 |
| HphI | GGTGA | 1 cut(s) | 45 |
| Hpy188I | TCNGA | 4 cut(s) | 139, 252, 280, 442 |
| Hpy188III | TCNNGA | 3 cut(s) | 41, 263, 520 |
| HpyAV | CCTTC | 2 cut(s) | 37, 260 |
| HpyCH4III | ACNGT | 2 cut(s) | 220, 763 |
| HpyCH4IV | ACGT | 1 cut(s) | 705 |
| HpyCH4V | TGCA | 6 cut(s) | 73, 92, 169, 410, 601, 881 |
| HpyF3I | CTNAG | 1 cut(s) | 754 |
| HpySE526I | ACGT | 1 cut(s) | 705 |
| Hsp92II | CATG | 9 cut(s) | 88, 304, 410, 464, 511, 553, 601, 725, 885 |
| HspAI | GCGC | 3 cut(s) | 82, 447, 797 |
| Kpn2I | TCCGGA | 1 cut(s) | 262 |
| KspI | CCGCGG | 1 cut(s) | 543 |
| Kzo9I | GATC | 4 cut(s) | 354, 388, 429, 652 |
| LmnI | GCTCC | 2 cut(s) | 18, 269 |
| Lsp1109I | GCAGC | 8 cut(s) | 8, 57, 70, 76, 696, 730, 747, 825 |
| LweI | GCATC | 2 cut(s) | 868, 871 |
| MaeI | CTAG | 5 cut(s) | 18, 386, 420, 477, 732 |
| MaeII | ACGT | 1 cut(s) | 705 |
| MaeIII | GTNAC | 1 cut(s) | 51 |
| MalI | GATC | 4 cut(s) | 356, 390, 431, 654 |
| MboI | GATC | 4 cut(s) | 354, 388, 429, 652 |
| MboII | GAAGA | 4 cut(s) | 340, 551, 739, 793 |
| MflI | RGATCY | 1 cut(s) | 652 |
| MhlI | GDGCHC | 2 cut(s) | 8, 460 |
| MlsI | TGGCCA | 2 cut(s) | 596, 681 |
| MluCI | AATT | 2 cut(s) | 396, 611 |
| MluNI | TGGCCA | 2 cut(s) | 596, 681 |
| MlyI | GAGTC | 1 cut(s) | 110 |
| Mox20I | TGGCCA | 2 cut(s) | 596, 681 |
| Mph1103I | ATGCAT | 1 cut(s) | 883 |
| MroI | TCCGGA | 1 cut(s) | 262 |
| MscI | TGGCCA | 2 cut(s) | 596, 681 |
| MseI | TTAA | 1 cut(s) | 153 |
| Msp20I | TGGCCA | 2 cut(s) | 596, 681 |
| MspA1I | CMGCKG | 5 cut(s) | 61, 338, 542, 778, 810 |
| MspCI | CTTAAG | 1 cut(s) | 152 |
| MspI | CCGG | 2 cut(s) | 263, 690 |
| MvnI | CGCG | 4 cut(s) | 381, 449, 467, 542 |
| NdeII | GATC | 4 cut(s) | 354, 388, 429, 652 |
| NheI | GCTAGC | 1 cut(s) | 476 |
| NlaIII | CATG | 9 cut(s) | 88, 304, 410, 464, 511, 553, 601, 725, 885 |
| NlaIV | GGNNCC | 3 cut(s) | 211, 458, 654 |
| NmuCI | GTSAC | 1 cut(s) | 51 |
| NsbI | TGCGCA | 1 cut(s) | 83 |
| NsiI | ATGCAT | 1 cut(s) | 883 |
| NspI | RCATGY | 1 cut(s) | 410 |
| PceI | AGGCCT | 1 cut(s) | 869 |
| PfeI | GAWTC | 1 cut(s) | 487 |
| PleI | GAGTC | 1 cut(s) | 110 |
| PpsI | GAGTC | 1 cut(s) | 110 |
| Ppu21I | YACGTR | 1 cut(s) | 706 |
| Psp124BI | GAGCTC | 1 cut(s) | 8 |
| PspN4I | GGNNCC | 3 cut(s) | 211, 458, 654 |
| PspOMI | GGGCCC | 1 cut(s) | 456 |
| PspPI | GGNCC | 2 cut(s) | 456, 457 |
| PstI | CTGCAG | 1 cut(s) | 75 |
| PstNI | CAGNNNCTG | 1 cut(s) | 761 |
| PsuI | RGATCY | 1 cut(s) | 652 |
| PvuII | CAGCTG | 1 cut(s) | 61 |
| RsaI | GTAC | 2 cut(s) | 694, 704 |
| RsaNI | GTAC | 2 cut(s) | 693, 703 |
| SacI | GAGCTC | 1 cut(s) | 8 |
| SacII | CCGCGG | 1 cut(s) | 543 |
| SaqAI | TTAA | 1 cut(s) | 153 |
| Sau3AI | GATC | 4 cut(s) | 354, 388, 429, 652 |
| Sau96I | GGNCC | 2 cut(s) | 456, 457 |
| SchI | GAGTC | 1 cut(s) | 110 |
| SduI | GDGCHC | 2 cut(s) | 8, 460 |
| SfaNI | GCATC | 2 cut(s) | 868, 871 |
| SfcI | CTRYAG | 1 cut(s) | 71 |
| Sfr303I | CCGCGG | 1 cut(s) | 543 |
| SgrBI | CCGCGG | 1 cut(s) | 543 |
| SmlI | CTYRAG | 2 cut(s) | 126, 152 |
| SmoI | CTYRAG | 2 cut(s) | 126, 152 |
| Sse9I | AATT | 2 cut(s) | 396, 611 |
| SseBI | AGGCCT | 1 cut(s) | 869 |
| SsiI | CCGC | 9 cut(s) | 56, 336, 449, 465, 540, 542, 776, 810, 840 |
| SspI | AATATT | 1 cut(s) | 310 |
| SspMI | CTAG | 5 cut(s) | 18, 386, 420, 477, 732 |
| SstI | GAGCTC | 1 cut(s) | 8 |
| StuI | AGGCCT | 1 cut(s) | 869 |
| StyI | CCWWGG | 1 cut(s) | 667 |
| TaaI | ACNGT | 2 cut(s) | 220, 763 |
| TaiI | ACGT | 1 cut(s) | 708 |
| TasI | AATT | 2 cut(s) | 396, 611 |
| TauI | GCSGC | 4 cut(s) | 338, 467, 545, 813 |
| TfiI | GAWTC | 1 cut(s) | 487 |
| Tru1I | TTAA | 1 cut(s) | 153 |
| Tru9I | TTAA | 1 cut(s) | 153 |
| TscAI | CASTG | 2 cut(s) | 225, 497 |
| TseFI | GTSAC | 1 cut(s) | 51 |
| TseI | GCWGC | 8 cut(s) | 21, 58, 70, 89, 709, 735, 743, 813 |
| Tsp45I | GTSAC | 1 cut(s) | 51 |
| TspRI | CASTG | 2 cut(s) | 225, 497 |
| Vha464I | CTTAAG | 1 cut(s) | 152 |
| XceI | RCATGY | 1 cut(s) | 410 |
| XspI | CTAG | 5 cut(s) | 18, 386, 420, 477, 732 |
| Zsp2I | ATGCAT | 1 cut(s) | 883 |