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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 171 |
| AccII | CGCG | 2 cut(s) | 11, 285 |
| AciI | CCGC | 8 cut(s) | 9, 107, 165, 169, 180, 224, 299, 318 |
| AclWI | GGATC | 1 cut(s) | 309 |
| AcoI | YGGCCR | 1 cut(s) | 174 |
| AfaI | GTAC | 1 cut(s) | 203 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 249 |
| AgsI | TTSAA | 1 cut(s) | 77 |
| AlwI | GGATC | 1 cut(s) | 309 |
| AoxI | GGCC | 2 cut(s) | 174, 276 |
| ApeKI | GCWGC | 1 cut(s) | 296 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 17 |
| AsuHPI | GGTGA | 1 cut(s) | 37 |
| BaeGI | GKGCMC | 1 cut(s) | 311 |
| BbvI | GCAGC | 1 cut(s) | 283 |
| BccI | CCATC | 2 cut(s) | 191, 354 |
| BceAI | ACGGC | 1 cut(s) | 275 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 173 |
| BisI | GCNGC | 3 cut(s) | 9, 108, 297 |
| BlpI | GCTNAGC | 1 cut(s) | 377 |
| BlsI | GCNGC | 3 cut(s) | 10, 109, 298 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 377 |
| BsaJI | CCNNGG | 1 cut(s) | 94 |
| BsaWI | WCCGGW | 1 cut(s) | 206 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 249 |
| BseDI | CCNNGG | 1 cut(s) | 94 |
| BseGI | GGATG | 1 cut(s) | 160 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 249 |
| BseSI | GKGCMC | 1 cut(s) | 311 |
| BseXI | GCAGC | 1 cut(s) | 283 |
| Bsh1236I | CGCG | 2 cut(s) | 11, 285 |
| BshFI | GGCC | 2 cut(s) | 176, 278 |
| BsiSI | CCGG | 1 cut(s) | 207 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 249 |
| BsnI | GGCC | 2 cut(s) | 176, 278 |
| Bsp1286I | GDGCHC | 1 cut(s) | 311 |
| Bsp143I | GATC | 2 cut(s) | 3, 301 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 377 |
| BspACI | CCGC | 8 cut(s) | 9, 107, 165, 169, 180, 224, 299, 318 |
| BspANI | GGCC | 2 cut(s) | 176, 278 |
| BspFNI | CGCG | 2 cut(s) | 11, 285 |
| BspPI | GGATC | 1 cut(s) | 309 |
| BsrBI | CCGCTC | 1 cut(s) | 171 |
| BssECI | CCNNGG | 1 cut(s) | 94 |
| BssMI | GATC | 2 cut(s) | 3, 301 |
| Bst4CI | ACNGT | 1 cut(s) | 98 |
| Bst6I | CTCTTC | 1 cut(s) | 242 |
| BstC8I | GCNNGC | 1 cut(s) | 169 |
| BstDEI | CTNAG | 1 cut(s) | 377 |
| BstDSI | CCRYGG | 1 cut(s) | 94 |
| BstF5I | GGATG | 1 cut(s) | 160 |
| BstFNI | CGCG | 2 cut(s) | 11, 285 |
| BstKTI | GATC | 2 cut(s) | 6, 304 |
| BstMBI | GATC | 2 cut(s) | 3, 301 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 173, 315, 373 |
| BstSLI | GKGCMC | 1 cut(s) | 311 |
| BstUI | CGCG | 2 cut(s) | 11, 285 |
| BstV1I | GCAGC | 1 cut(s) | 283 |
| BsuRI | GGCC | 2 cut(s) | 176, 278 |
| BtgI | CCRYGG | 1 cut(s) | 94 |
| BtsCI | GGATG | 1 cut(s) | 160 |
| Cac8I | GCNNGC | 1 cut(s) | 169 |
| Csp6I | GTAC | 1 cut(s) | 202 |
| CviJI | RGCY | 8 cut(s) | 102, 124, 155, 176, 237, 278, 296, 381 |
| CviKI_1 | RGCY | 8 cut(s) | 102, 124, 155, 176, 237, 278, 296, 381 |
| CviQI | GTAC | 1 cut(s) | 202 |
| DdeI | CTNAG | 1 cut(s) | 377 |
| DpnI | GATC | 2 cut(s) | 5, 303 |
| DpnII | GATC | 2 cut(s) | 3, 301 |
| EaeI | YGGCCR | 1 cut(s) | 174 |
| Eam1104I | CTCTTC | 1 cut(s) | 242 |
| EarI | CTCTTC | 1 cut(s) | 242 |
| FauI | CCCGC | 2 cut(s) | 172, 176 |
| Fnu4HI | GCNGC | 3 cut(s) | 9, 108, 297 |
| FokI | GGATG | 1 cut(s) | 147 |
| Fsp4HI | GCNGC | 3 cut(s) | 9, 108, 297 |
| GluI | GCNGC | 3 cut(s) | 9, 108, 297 |
| HaeIII | GGCC | 2 cut(s) | 176, 278 |
| HapII | CCGG | 1 cut(s) | 207 |
| HinfI | GANTC | 2 cut(s) | 42, 63 |
| HpaII | CCGG | 1 cut(s) | 207 |
| HphI | GGTGA | 1 cut(s) | 37 |
| Hpy166II | GTNNAC | 1 cut(s) | 204 |
| Hpy188I | TCNGA | 1 cut(s) | 341 |
| Hpy188III | TCNNGA | 2 cut(s) | 22, 351 |
| Hpy8I | GTNNAC | 1 cut(s) | 204 |
| HpyAV | CCTTC | 6 cut(s) | 109, 185, 208, 214, 249, 348 |
| HpyCH4III | ACNGT | 1 cut(s) | 98 |
| HpyCH4V | TGCA | 1 cut(s) | 324 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 173, 315, 373 |
| HpyF3I | CTNAG | 1 cut(s) | 377 |
| Kzo9I | GATC | 2 cut(s) | 3, 301 |
| LpnPI | CCDG | 1 cut(s) | 220 |
| Lsp1109I | GCAGC | 1 cut(s) | 283 |
| MaeIII | GTNAC | 1 cut(s) | 43 |
| MalI | GATC | 2 cut(s) | 5, 303 |
| MbiI | CCGCTC | 1 cut(s) | 171 |
| MboI | GATC | 2 cut(s) | 3, 301 |
| MboII | GAAGA | 2 cut(s) | 25, 259 |
| MfeI | CAATTG | 1 cut(s) | 325 |
| MhlI | GDGCHC | 1 cut(s) | 311 |
| MluCI | AATT | 1 cut(s) | 325 |
| MlyI | GAGTC | 2 cut(s) | 51, 57 |
| MmeI | TCCRAC | 2 cut(s) | 91, 364 |
| MnlI | CCTC | 6 cut(s) | 105, 114, 243, 268, 286, 357 |
| MroXI | GAANNNNTTC | 1 cut(s) | 17 |
| MseI | TTAA | 1 cut(s) | 385 |
| MspI | CCGG | 1 cut(s) | 207 |
| MunI | CAATTG | 1 cut(s) | 325 |
| MvnI | CGCG | 2 cut(s) | 11, 285 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 173, 315, 373 |
| NdeII | GATC | 2 cut(s) | 3, 301 |
| NmeAIII | GCCGAG | 2 cut(s) | 83, 152 |
| NmuCI | GTSAC | 1 cut(s) | 43 |
| PdmI | GAANNNNTTC | 1 cut(s) | 17 |
| PkrI | GCNGC | 3 cut(s) | 10, 109, 298 |
| PleI | GAGTC | 2 cut(s) | 50, 57 |
| PpsI | GAGTC | 2 cut(s) | 50, 57 |
| RsaI | GTAC | 1 cut(s) | 203 |
| RsaNI | GTAC | 1 cut(s) | 202 |
| SaqAI | TTAA | 1 cut(s) | 385 |
| SatI | GCNGC | 3 cut(s) | 9, 108, 297 |
| Sau3AI | GATC | 2 cut(s) | 3, 301 |
| SchI | GAGTC | 2 cut(s) | 51, 57 |
| SduI | GDGCHC | 1 cut(s) | 311 |
| SetI | ASST | 1 cut(s) | 269 |
| Sse9I | AATT | 1 cut(s) | 325 |
| SsiI | CCGC | 8 cut(s) | 9, 107, 165, 169, 180, 224, 299, 318 |
| TaaI | ACNGT | 1 cut(s) | 98 |
| TaqI | TCGA | 3 cut(s) | 21, 147, 350 |
| TasI | AATT | 1 cut(s) | 325 |
| TauI | GCSGC | 2 cut(s) | 11, 110 |
| Tru1I | TTAA | 1 cut(s) | 385 |
| Tru9I | TTAA | 1 cut(s) | 385 |
| TseFI | GTSAC | 1 cut(s) | 43 |
| TseI | GCWGC | 1 cut(s) | 296 |
| Tsp45I | GTSAC | 1 cut(s) | 43 |
| XmnI | GAANNNNTTC | 1 cut(s) | 17 |