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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 235 |
| Acc36I | ACCTGC | 1 cut(s) | 575 |
| AccII | CGCG | 3 cut(s) | 167, 411, 551 |
| AciI | CCGC | 5 cut(s) | 167, 267, 324, 774, 870 |
| AclWI | GGATC | 1 cut(s) | 690 |
| AcoI | YGGCCR | 1 cut(s) | 624 |
| AfaI | GTAC | 3 cut(s) | 465, 734, 772 |
| AflIII | ACRYGT | 2 cut(s) | 234, 735 |
| AgsI | TTSAA | 2 cut(s) | 38, 667 |
| AjnI | CCWGG | 2 cut(s) | 297, 648 |
| AluBI | AGCT | 4 cut(s) | 34, 403, 674, 827 |
| AluI | AGCT | 4 cut(s) | 34, 403, 674, 827 |
| Alw21I | GWGCWC | 2 cut(s) | 18, 405 |
| Alw26I | GTCTC | 1 cut(s) | 334 |
| AlwI | GGATC | 1 cut(s) | 690 |
| AoxI | GGCC | 3 cut(s) | 300, 624, 897 |
| ApeKI | GCWGC | 4 cut(s) | 454, 765, 840, 843 |
| AspLEI | GCGC | 1 cut(s) | 426 |
| AsuHPI | GGTGA | 2 cut(s) | 251, 855 |
| BalI | TGGCCA | 1 cut(s) | 626 |
| BanII | GRGCYC | 2 cut(s) | 405, 490 |
| Bbv12I | GWGCWC | 2 cut(s) | 18, 405 |
| BbvI | GCAGC | 4 cut(s) | 466, 777, 827, 855 |
| BccI | CCATC | 1 cut(s) | 593 |
| BceAI | ACGGC | 1 cut(s) | 408 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 29 |
| BciT130I | CCWGG | 2 cut(s) | 299, 650 |
| BciVI | GTATCC | 1 cut(s) | 439 |
| BcoDI | GTCTC | 1 cut(s) | 334 |
| BfaI | CTAG | 3 cut(s) | 416, 507, 901 |
| BfmI | CTRYAG | 1 cut(s) | 841 |
| BfuAI | ACCTGC | 1 cut(s) | 575 |
| BfuI | GTATCC | 1 cut(s) | 439 |
| BisI | GCNGC | 4 cut(s) | 455, 766, 841, 844 |
| BlsI | GCNGC | 4 cut(s) | 456, 767, 842, 845 |
| Bme1390I | CCNGG | 2 cut(s) | 299, 650 |
| BmiI | GGNNCC | 1 cut(s) | 703 |
| BmrFI | CCNGG | 2 cut(s) | 299, 650 |
| BmsI | GCATC | 1 cut(s) | 404 |
| BpmI | CTGGAG | 1 cut(s) | 127 |
| BsaAI | YACGTR | 1 cut(s) | 736 |
| BsaJI | CCNNGG | 4 cut(s) | 297, 495, 618, 697 |
| Bse1I | ACTGG | 2 cut(s) | 144, 303 |
| BseBI | CCWGG | 2 cut(s) | 299, 650 |
| BseDI | CCNNGG | 4 cut(s) | 297, 495, 618, 697 |
| BseGI | GGATG | 3 cut(s) | 23, 395, 604 |
| BseMII | CTCAG | 1 cut(s) | 264 |
| BseNI | ACTGG | 2 cut(s) | 144, 303 |
| BseRI | GAGGAG | 1 cut(s) | 10 |
| BseXI | GCAGC | 4 cut(s) | 466, 777, 827, 855 |
| BsgI | GTGCAG | 1 cut(s) | 58 |
| Bsh1236I | CGCG | 3 cut(s) | 167, 411, 551 |
| Bsh1285I | CGRYCG | 1 cut(s) | 267 |
| BshFI | GGCC | 3 cut(s) | 302, 626, 899 |
| BsiEI | CGRYCG | 1 cut(s) | 267 |
| BsiHKAI | GWGCWC | 2 cut(s) | 18, 405 |
| BslFI | GGGAC | 1 cut(s) | 71 |
| BsmAI | GTCTC | 1 cut(s) | 334 |
| BsmFI | GGGAC | 1 cut(s) | 71 |
| BsmI | GAATGC | 2 cut(s) | 157, 481 |
| BsnI | GGCC | 3 cut(s) | 302, 626, 899 |
| Bsp1286I | GDGCHC | 3 cut(s) | 18, 405, 490 |
| Bsp143I | GATC | 1 cut(s) | 682 |
| Bsp68I | TCGCGA | 1 cut(s) | 551 |
| BspACI | CCGC | 5 cut(s) | 167, 267, 324, 774, 870 |
| BspANI | GGCC | 3 cut(s) | 302, 626, 899 |
| BspCNI | CTCAG | 1 cut(s) | 265 |
| BspFNI | CGCG | 3 cut(s) | 167, 411, 551 |
| BspLI | GGNNCC | 1 cut(s) | 703 |
| BspMAI | CTGCAG | 1 cut(s) | 845 |
| BspMI | ACCTGC | 1 cut(s) | 575 |
| BspPI | GGATC | 1 cut(s) | 690 |
| BsrI | ACTGG | 2 cut(s) | 144, 303 |
| BssECI | CCNNGG | 4 cut(s) | 297, 495, 618, 697 |
| BssMI | GATC | 1 cut(s) | 682 |
| BssT1I | CCWWGG | 1 cut(s) | 697 |
| Bst2UI | CCWGG | 2 cut(s) | 299, 650 |
| Bst4CI | ACNGT | 3 cut(s) | 468, 717, 793 |
| Bst6I | CTCTTC | 1 cut(s) | 246 |
| BstBAI | YACGTR | 1 cut(s) | 736 |
| BstC8I | GCNNGC | 1 cut(s) | 825 |
| BstDEI | CTNAG | 3 cut(s) | 273, 450, 590 |
| BstDSI | CCRYGG | 1 cut(s) | 495 |
| BstF5I | GGATG | 3 cut(s) | 23, 395, 604 |
| BstFNI | CGCG | 3 cut(s) | 167, 411, 551 |
| BstHHI | GCGC | 1 cut(s) | 426 |
| BstKTI | GATC | 1 cut(s) | 685 |
| BstMAI | GTCTC | 1 cut(s) | 334 |
| BstMBI | GATC | 1 cut(s) | 682 |
| BstMCI | CGRYCG | 1 cut(s) | 267 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 485, 611, 708, 748 |
| BstNI | CCWGG | 2 cut(s) | 299, 650 |
| BstNSI | RCATGY | 2 cut(s) | 238, 440 |
| BstSCI | CCNGG | 2 cut(s) | 297, 648 |
| BstSFI | CTRYAG | 1 cut(s) | 841 |
| BstUI | CGCG | 3 cut(s) | 167, 411, 551 |
| BstV1I | GCAGC | 4 cut(s) | 466, 777, 827, 855 |
| BstX2I | RGATCY | 1 cut(s) | 682 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 340, 719 |
| BstYI | RGATCY | 1 cut(s) | 682 |
| BsuI | GTATCC | 1 cut(s) | 439 |
| BsuRI | GGCC | 3 cut(s) | 302, 626, 899 |
| BtgI | CCRYGG | 1 cut(s) | 495 |
| BtsCI | GGATG | 3 cut(s) | 23, 395, 604 |
| BtsIMutI | CAGTG | 3 cut(s) | 310, 527, 806 |
| BtuMI | TCGCGA | 1 cut(s) | 551 |
| BveI | ACCTGC | 1 cut(s) | 575 |
| Cac8I | GCNNGC | 1 cut(s) | 825 |
| CfoI | GCGC | 1 cut(s) | 426 |
| Csp6I | GTAC | 3 cut(s) | 464, 733, 771 |
| CspCI | CAANNNNNGTGG | 8 cut(s) | 16, 51, 603, 638, 775, 778, 810, 813 |
| CviAII | CATG | 5 cut(s) | 235, 437, 491, 538, 867 |
| CviQI | GTAC | 3 cut(s) | 464, 733, 771 |
| DdeI | CTNAG | 3 cut(s) | 273, 450, 590 |
| DpnI | GATC | 1 cut(s) | 684 |
| DpnII | GATC | 1 cut(s) | 682 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 235 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 235 |
| EaeI | YGGCCR | 1 cut(s) | 624 |
| Eam1104I | CTCTTC | 1 cut(s) | 246 |
| EarI | CTCTTC | 1 cut(s) | 246 |
| Ecl136II | GAGCTC | 1 cut(s) | 403 |
| Eco130I | CCWWGG | 1 cut(s) | 697 |
| Eco147I | AGGCCT | 1 cut(s) | 899 |
| Eco24I | GRGCYC | 2 cut(s) | 405, 490 |
| Eco53kI | GAGCTC | 1 cut(s) | 403 |
| EcoICRI | GAGCTC | 1 cut(s) | 403 |
| EcoRII | CCWGG | 2 cut(s) | 297, 648 |
| EcoT14I | CCWWGG | 1 cut(s) | 697 |
| EcoT38I | GRGCYC | 2 cut(s) | 405, 490 |
| ErhI | CCWWGG | 1 cut(s) | 697 |
| FaeI | CATG | 5 cut(s) | 238, 440, 494, 541, 870 |
| FaiI | YATR | 9 cut(s) | 236, 338, 407, 438, 492, 539, 581, 629, 868 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 346, 378 |
| FaqI | GGGAC | 1 cut(s) | 71 |
| FatI | CATG | 5 cut(s) | 234, 436, 490, 537, 866 |
| Fnu4HI | GCNGC | 4 cut(s) | 455, 766, 841, 844 |
| FokI | GGATG | 3 cut(s) | 10, 382, 611 |
| FriOI | GRGCYC | 2 cut(s) | 405, 490 |
| Fsp4HI | GCNGC | 4 cut(s) | 455, 766, 841, 844 |
| FspBI | CTAG | 3 cut(s) | 416, 507, 901 |
| GlaI | GCGC | 1 cut(s) | 425 |
| GluI | GCNGC | 4 cut(s) | 455, 766, 841, 844 |
| GsuI | CTGGAG | 1 cut(s) | 127 |
| HaeIII | GGCC | 3 cut(s) | 302, 626, 899 |
| HhaI | GCGC | 1 cut(s) | 426 |
| Hin1II | CATG | 5 cut(s) | 238, 440, 494, 541, 870 |
| Hin6I | GCGC | 1 cut(s) | 424 |
| HinP1I | GCGC | 1 cut(s) | 424 |
| HincII | GTYRAC | 1 cut(s) | 240 |
| HindII | GTYRAC | 1 cut(s) | 240 |
| HindIII | AAGCTT | 2 cut(s) | 32, 672 |
| HinfI | GANTC | 5 cut(s) | 59, 199, 256, 517, 745 |
| HphI | GGTGA | 2 cut(s) | 251, 855 |
| Hpy166II | GTNNAC | 2 cut(s) | 115, 240 |
| Hpy188I | TCNGA | 4 cut(s) | 282, 400, 472, 577 |
| Hpy188III | TCNNGA | 2 cut(s) | 383, 550 |
| Hpy8I | GTNNAC | 2 cut(s) | 115, 240 |
| Hpy99I | CGWCG | 1 cut(s) | 71 |
| HpyAV | CCTTC | 2 cut(s) | 751, 792 |
| HpyCH4III | ACNGT | 3 cut(s) | 468, 717, 793 |
| HpyCH4IV | ACGT | 2 cut(s) | 572, 735 |
| HpyCH4V | TGCA | 5 cut(s) | 75, 440, 479, 614, 843 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 485, 611, 708, 748 |
| HpyF3I | CTNAG | 3 cut(s) | 273, 450, 590 |
| HpySE526I | ACGT | 2 cut(s) | 572, 735 |
| Hsp92II | CATG | 5 cut(s) | 238, 440, 494, 541, 870 |
| HspAI | GCGC | 1 cut(s) | 424 |
| Kzo9I | GATC | 1 cut(s) | 682 |
| LmnI | GCTCC | 1 cut(s) | 707 |
| Lsp1109I | GCAGC | 4 cut(s) | 466, 777, 827, 855 |
| LweI | GCATC | 1 cut(s) | 404 |
| MaeI | CTAG | 3 cut(s) | 416, 507, 901 |
| MaeII | ACGT | 2 cut(s) | 572, 735 |
| MaeIII | GTNAC | 2 cut(s) | 146, 196 |
| MalI | GATC | 1 cut(s) | 684 |
| MboI | GATC | 1 cut(s) | 682 |
| MboII | GAAGA | 3 cut(s) | 50, 53, 263 |
| MfeI | CAATTG | 1 cut(s) | 224 |
| MflI | RGATCY | 1 cut(s) | 682 |
| MhlI | GDGCHC | 3 cut(s) | 18, 405, 490 |
| MlsI | TGGCCA | 1 cut(s) | 626 |
| MluCI | AATT | 2 cut(s) | 224, 641 |
| MluNI | TGGCCA | 1 cut(s) | 626 |
| MlyI | GAGTC | 2 cut(s) | 53, 193 |
| Mox20I | TGGCCA | 1 cut(s) | 626 |
| MscI | TGGCCA | 1 cut(s) | 626 |
| MseI | TTAA | 3 cut(s) | 644, 677, 687 |
| Msp20I | TGGCCA | 1 cut(s) | 626 |
| MspA1I | CMGCKG | 1 cut(s) | 326 |
| MspR9I | CCNGG | 2 cut(s) | 299, 650 |
| MunI | CAATTG | 1 cut(s) | 224 |
| Mva1269I | GAATGC | 2 cut(s) | 157, 481 |
| MvaI | CCWGG | 2 cut(s) | 299, 650 |
| MvnI | CGCG | 3 cut(s) | 167, 411, 551 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 485, 611, 708, 748 |
| NdeII | GATC | 1 cut(s) | 682 |
| NlaIII | CATG | 5 cut(s) | 238, 440, 494, 541, 870 |
| NlaIV | GGNNCC | 1 cut(s) | 703 |
| NmuCI | GTSAC | 1 cut(s) | 196 |
| NruI | TCGCGA | 1 cut(s) | 551 |
| NspI | RCATGY | 2 cut(s) | 238, 440 |
| PceI | AGGCCT | 1 cut(s) | 899 |
| PciI | ACATGT | 1 cut(s) | 234 |
| PctI | GAATGC | 2 cut(s) | 157, 481 |
| PfeI | GAWTC | 3 cut(s) | 256, 517, 745 |
| PflFI | GACNNNGTC | 1 cut(s) | 571 |
| PkrI | GCNGC | 4 cut(s) | 456, 767, 842, 845 |
| PleI | GAGTC | 2 cut(s) | 53, 193 |
| PpsI | GAGTC | 2 cut(s) | 53, 193 |
| Ppu21I | YACGTR | 1 cut(s) | 736 |
| PscI | ACATGT | 1 cut(s) | 234 |
| Psp124BI | GAGCTC | 1 cut(s) | 405 |
| Psp6I | CCWGG | 2 cut(s) | 297, 648 |
| PspGI | CCWGG | 2 cut(s) | 297, 648 |
| PspN4I | GGNNCC | 1 cut(s) | 703 |
| PstI | CTGCAG | 1 cut(s) | 845 |
| PsuI | RGATCY | 1 cut(s) | 682 |
| PsyI | GACNNNGTC | 1 cut(s) | 571 |
| RruI | TCGCGA | 1 cut(s) | 551 |
| RsaI | GTAC | 3 cut(s) | 465, 734, 772 |
| RsaNI | GTAC | 3 cut(s) | 464, 733, 771 |
| SacI | GAGCTC | 1 cut(s) | 405 |
| SaqAI | TTAA | 3 cut(s) | 644, 677, 687 |
| SatI | GCNGC | 4 cut(s) | 455, 766, 841, 844 |
| Sau3AI | GATC | 1 cut(s) | 682 |
| SchI | GAGTC | 2 cut(s) | 53, 193 |
| ScrFI | CCNGG | 2 cut(s) | 299, 650 |
| SduI | GDGCHC | 3 cut(s) | 18, 405, 490 |
| SfaNI | GCATC | 1 cut(s) | 404 |
| SfcI | CTRYAG | 1 cut(s) | 841 |
| Sse9I | AATT | 2 cut(s) | 224, 641 |
| SseBI | AGGCCT | 1 cut(s) | 899 |
| SsiI | CCGC | 5 cut(s) | 167, 267, 324, 774, 870 |
| SspMI | CTAG | 3 cut(s) | 416, 507, 901 |
| SstI | GAGCTC | 1 cut(s) | 405 |
| StuI | AGGCCT | 1 cut(s) | 899 |
| StyD4I | CCNGG | 2 cut(s) | 297, 648 |
| StyI | CCWWGG | 1 cut(s) | 697 |
| TaaI | ACNGT | 3 cut(s) | 468, 717, 793 |
| TaiI | ACGT | 2 cut(s) | 575, 738 |
| TaqII | GACCGA | 1 cut(s) | 245 |
| TasI | AATT | 2 cut(s) | 224, 641 |
| TfiI | GAWTC | 3 cut(s) | 256, 517, 745 |
| Tru1I | TTAA | 3 cut(s) | 644, 677, 687 |
| Tru9I | TTAA | 3 cut(s) | 644, 677, 687 |
| TscAI | CASTG | 3 cut(s) | 310, 527, 813 |
| TseFI | GTSAC | 1 cut(s) | 196 |
| TseI | GCWGC | 4 cut(s) | 454, 765, 840, 843 |
| Tsp45I | GTSAC | 1 cut(s) | 196 |
| TspGWI | ACGGA | 1 cut(s) | 97 |
| TspRI | CASTG | 3 cut(s) | 310, 527, 813 |
| Tth111I | GACNNNGTC | 1 cut(s) | 571 |
| XceI | RCATGY | 2 cut(s) | 238, 440 |
| XspI | CTAG | 3 cut(s) | 416, 507, 901 |