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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 150 |
| Acc36I | ACCTGC | 1 cut(s) | 577 |
| AccI | GTMKAC | 1 cut(s) | 653 |
| AccII | CGCG | 3 cut(s) | 82, 84, 329 |
| AciI | CCGC | 4 cut(s) | 308, 327, 623, 730 |
| AclI | AACGTT | 1 cut(s) | 333 |
| AclWI | GGATC | 1 cut(s) | 453 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcsI | RAATTY | 1 cut(s) | 128 |
| AcyI | GRCGYC | 1 cut(s) | 147 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 46 |
| AflIII | ACRYGT | 2 cut(s) | 75, 681 |
| AgsI | TTSAA | 2 cut(s) | 189, 305 |
| AjiI | CACGTC | 1 cut(s) | 682 |
| AjnI | CCWGG | 1 cut(s) | 384 |
| AluBI | AGCT | 5 cut(s) | 394, 427, 542, 709, 750 |
| AluI | AGCT | 5 cut(s) | 394, 427, 542, 709, 750 |
| Alw21I | GWGCWC | 3 cut(s) | 75, 316, 429 |
| Alw26I | GTCTC | 2 cut(s) | 138, 583 |
| AlwI | GGATC | 1 cut(s) | 453 |
| Ama87I | CYCGRG | 1 cut(s) | 278 |
| AoxI | GGCC | 2 cut(s) | 324, 672 |
| ApeKI | GCWGC | 2 cut(s) | 406, 542 |
| ApoI | RAATTY | 1 cut(s) | 128 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 101, 133 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 476 |
| AspLEI | GCGC | 2 cut(s) | 84, 406 |
| AspS9I | GGNCC | 2 cut(s) | 673, 727 |
| AsuHPI | GGTGA | 1 cut(s) | 494 |
| AvaI | CYCGRG | 1 cut(s) | 278 |
| AvaII | GGWCC | 1 cut(s) | 727 |
| BanII | GRGCYC | 1 cut(s) | 429 |
| BbsI | GAAGAC | 1 cut(s) | 384 |
| Bbv12I | GWGCWC | 3 cut(s) | 75, 316, 429 |
| BbvI | GCAGC | 2 cut(s) | 393, 529 |
| BccI | CCATC | 1 cut(s) | 257 |
| BciT130I | CCWGG | 1 cut(s) | 386 |
| BcoDI | GTCTC | 2 cut(s) | 138, 583 |
| BfaI | CTAG | 3 cut(s) | 56, 176, 573 |
| BfuAI | ACCTGC | 1 cut(s) | 577 |
| BglII | AGATCT | 1 cut(s) | 612 |
| BisI | GCNGC | 4 cut(s) | 327, 407, 543, 624 |
| BlpI | GCTNAGC | 1 cut(s) | 69 |
| BlsI | GCNGC | 4 cut(s) | 328, 408, 544, 625 |
| Bme1390I | CCNGG | 1 cut(s) | 386 |
| Bme18I | GGWCC | 1 cut(s) | 727 |
| BmeT110I | CYCGRG | 1 cut(s) | 278 |
| BmgBI | CACGTC | 1 cut(s) | 682 |
| BmgT120I | GGNCC | 2 cut(s) | 673, 727 |
| BmiI | GGNNCC | 1 cut(s) | 243 |
| BmrFI | CCNGG | 1 cut(s) | 386 |
| BmsI | GCATC | 1 cut(s) | 652 |
| BoxI | GACNNNNGTC | 1 cut(s) | 725 |
| BpiI | GAAGAC | 1 cut(s) | 384 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 69 |
| BsaAI | YACGTR | 1 cut(s) | 490 |
| BsaHI | GRCGYC | 1 cut(s) | 147 |
| BsaJI | CCNNGG | 1 cut(s) | 385 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 46 |
| Bse1I | ACTGG | 1 cut(s) | 296 |
| BseBI | CCWGG | 1 cut(s) | 386 |
| BseDI | CCNNGG | 1 cut(s) | 385 |
| BseGI | GGATG | 1 cut(s) | 381 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 46 |
| BseMII | CTCAG | 4 cut(s) | 60, 414, 426, 442 |
| BseNI | ACTGG | 1 cut(s) | 296 |
| BseRI | GAGGAG | 2 cut(s) | 545, 548 |
| BseXI | GCAGC | 2 cut(s) | 393, 529 |
| Bsh1236I | CGCG | 3 cut(s) | 82, 84, 329 |
| Bsh1285I | CGRYCG | 1 cut(s) | 400 |
| BshFI | GGCC | 2 cut(s) | 326, 674 |
| BsiEI | CGRYCG | 1 cut(s) | 400 |
| BsiHKAI | GWGCWC | 3 cut(s) | 75, 316, 429 |
| BsiHKCI | CYCGRG | 1 cut(s) | 278 |
| BslFI | GGGAC | 1 cut(s) | 62 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 46 |
| BsmAI | GTCTC | 2 cut(s) | 138, 583 |
| BsmBI | CGTCTC | 1 cut(s) | 138 |
| BsmFI | GGGAC | 1 cut(s) | 62 |
| BsnI | GGCC | 2 cut(s) | 326, 674 |
| BsoBI | CYCGRG | 1 cut(s) | 278 |
| Bsp1286I | GDGCHC | 3 cut(s) | 75, 316, 429 |
| Bsp143I | GATC | 2 cut(s) | 445, 612 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 69 |
| BspACI | CCGC | 4 cut(s) | 308, 327, 623, 730 |
| BspANI | GGCC | 2 cut(s) | 326, 674 |
| BspCNI | CTCAG | 4 cut(s) | 61, 415, 427, 441 |
| BspFNI | CGCG | 3 cut(s) | 82, 84, 329 |
| BspLI | GGNNCC | 1 cut(s) | 243 |
| BspMI | ACCTGC | 1 cut(s) | 577 |
| BspPI | GGATC | 1 cut(s) | 453 |
| BsrI | ACTGG | 1 cut(s) | 296 |
| BssECI | CCNNGG | 1 cut(s) | 385 |
| BssMI | GATC | 2 cut(s) | 445, 612 |
| BssNAI | GTATAC | 1 cut(s) | 654 |
| BssNI | GRCGYC | 1 cut(s) | 147 |
| Bst1107I | GTATAC | 1 cut(s) | 654 |
| Bst2UI | CCWGG | 1 cut(s) | 386 |
| Bst4CI | ACNGT | 3 cut(s) | 401, 512, 642 |
| BstACI | GRCGYC | 1 cut(s) | 147 |
| BstBAI | YACGTR | 1 cut(s) | 490 |
| BstC8I | GCNNGC | 2 cut(s) | 392, 563 |
| BstDEI | CTNAG | 6 cut(s) | 69, 416, 423, 428, 435, 633 |
| BstF5I | GGATG | 1 cut(s) | 381 |
| BstFNI | CGCG | 3 cut(s) | 82, 84, 329 |
| BstHHI | GCGC | 2 cut(s) | 84, 406 |
| BstKTI | GATC | 2 cut(s) | 448, 615 |
| BstMAI | GTCTC | 2 cut(s) | 138, 583 |
| BstMBI | GATC | 2 cut(s) | 445, 612 |
| BstMCI | CGRYCG | 1 cut(s) | 400 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 14, 79, 671 |
| BstNI | CCWGG | 1 cut(s) | 386 |
| BstNSI | RCATGY | 1 cut(s) | 79 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 725 |
| BstSCI | CCNGG | 1 cut(s) | 384 |
| BstUI | CGCG | 3 cut(s) | 82, 84, 329 |
| BstV1I | GCAGC | 2 cut(s) | 393, 529 |
| BstV2I | GAAGAC | 1 cut(s) | 384 |
| BstX2I | RGATCY | 2 cut(s) | 445, 612 |
| BstYI | RGATCY | 2 cut(s) | 445, 612 |
| BstZ17I | GTATAC | 1 cut(s) | 654 |
| BsuRI | GGCC | 2 cut(s) | 326, 674 |
| BtrI | CACGTC | 1 cut(s) | 682 |
| BtsCI | GGATG | 1 cut(s) | 381 |
| BveI | ACCTGC | 1 cut(s) | 577 |
| Cac8I | GCNNGC | 2 cut(s) | 392, 563 |
| CfoI | GCGC | 2 cut(s) | 84, 406 |
| Cfr13I | GGNCC | 2 cut(s) | 673, 727 |
| CseI | GACGC | 1 cut(s) | 73 |
| CviAII | CATG | 3 cut(s) | 76, 677, 718 |
| DdeI | CTNAG | 6 cut(s) | 69, 416, 423, 428, 435, 633 |
| DpnI | GATC | 2 cut(s) | 447, 614 |
| DpnII | GATC | 2 cut(s) | 445, 612 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Ecl136II | GAGCTC | 1 cut(s) | 427 |
| Eco24I | GRGCYC | 1 cut(s) | 429 |
| Eco47I | GGWCC | 1 cut(s) | 727 |
| Eco53kI | GAGCTC | 1 cut(s) | 427 |
| Eco88I | CYCGRG | 1 cut(s) | 278 |
| EcoICRI | GAGCTC | 1 cut(s) | 427 |
| EcoRII | CCWGG | 1 cut(s) | 384 |
| EcoT38I | GRGCYC | 1 cut(s) | 429 |
| Esp3I | CGTCTC | 1 cut(s) | 138 |
| FaeI | CATG | 3 cut(s) | 79, 680, 721 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 706, 738 |
| FaqI | GGGAC | 1 cut(s) | 62 |
| FatI | CATG | 3 cut(s) | 75, 676, 717 |
| FauI | CCCGC | 1 cut(s) | 301 |
| FblI | GTMKAC | 1 cut(s) | 653 |
| Fnu4HI | GCNGC | 4 cut(s) | 327, 407, 543, 624 |
| FokI | GGATG | 1 cut(s) | 368 |
| FriOI | GRGCYC | 1 cut(s) | 429 |
| Fsp4HI | GCNGC | 4 cut(s) | 327, 407, 543, 624 |
| FspBI | CTAG | 3 cut(s) | 56, 176, 573 |
| GlaI | GCGC | 2 cut(s) | 83, 405 |
| GluI | GCNGC | 4 cut(s) | 327, 407, 543, 624 |
| HaeIII | GGCC | 2 cut(s) | 326, 674 |
| HgaI | GACGC | 1 cut(s) | 73 |
| HhaI | GCGC | 2 cut(s) | 84, 406 |
| Hin1I | GRCGYC | 1 cut(s) | 147 |
| Hin1II | CATG | 3 cut(s) | 79, 680, 721 |
| Hin6I | GCGC | 2 cut(s) | 82, 404 |
| HinP1I | GCGC | 2 cut(s) | 82, 404 |
| HincII | GTYRAC | 1 cut(s) | 289 |
| HindII | GTYRAC | 1 cut(s) | 289 |
| HinfI | GANTC | 3 cut(s) | 472, 591, 601 |
| HpaI | GTTAAC | 1 cut(s) | 289 |
| HphI | GGTGA | 1 cut(s) | 494 |
| Hpy166II | GTNNAC | 5 cut(s) | 124, 289, 457, 630, 654 |
| Hpy188I | TCNGA | 2 cut(s) | 109, 431 |
| Hpy188III | TCNNGA | 2 cut(s) | 216, 616 |
| Hpy8I | GTNNAC | 5 cut(s) | 124, 289, 457, 630, 654 |
| Hpy99I | CGWCG | 1 cut(s) | 152 |
| HpyAV | CCTTC | 1 cut(s) | 251 |
| HpyCH4III | ACNGT | 3 cut(s) | 401, 512, 642 |
| HpyCH4IV | ACGT | 5 cut(s) | 147, 159, 333, 489, 681 |
| HpyCH4V | TGCA | 3 cut(s) | 138, 341, 409 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 14, 79, 671 |
| HpyF3I | CTNAG | 6 cut(s) | 69, 416, 423, 428, 435, 633 |
| HpySE526I | ACGT | 5 cut(s) | 147, 159, 333, 489, 681 |
| Hsp92I | GRCGYC | 1 cut(s) | 147 |
| Hsp92II | CATG | 3 cut(s) | 79, 680, 721 |
| HspAI | GCGC | 2 cut(s) | 82, 404 |
| KspAI | GTTAAC | 1 cut(s) | 289 |
| Kzo9I | GATC | 2 cut(s) | 445, 612 |
| LmnI | GCTCC | 1 cut(s) | 311 |
| Lsp1109I | GCAGC | 2 cut(s) | 393, 529 |
| LweI | GCATC | 1 cut(s) | 652 |
| MaeI | CTAG | 3 cut(s) | 56, 176, 573 |
| MaeII | ACGT | 5 cut(s) | 147, 159, 333, 489, 681 |
| MaeIII | GTNAC | 2 cut(s) | 85, 576 |
| MalI | GATC | 2 cut(s) | 447, 614 |
| MboI | GATC | 2 cut(s) | 445, 612 |
| MboII | GAAGA | 2 cut(s) | 389, 622 |
| MflI | RGATCY | 2 cut(s) | 445, 612 |
| MhlI | GDGCHC | 3 cut(s) | 75, 316, 429 |
| MluCI | AATT | 3 cut(s) | 33, 128, 551 |
| MlyI | GAGTC | 2 cut(s) | 585, 610 |
| MmeI | TCCRAC | 2 cut(s) | 87, 666 |
| MnlI | CCTC | 6 cut(s) | 172, 212, 523, 526, 529, 682 |
| MroXI | GAANNNNTTC | 1 cut(s) | 476 |
| MseI | TTAA | 3 cut(s) | 36, 288, 440 |
| MspA1I | CMGCKG | 2 cut(s) | 623, 750 |
| MspR9I | CCNGG | 1 cut(s) | 386 |
| MvaI | CCWGG | 1 cut(s) | 386 |
| MvnI | CGCG | 3 cut(s) | 82, 84, 329 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 14, 79, 671 |
| NdeII | GATC | 2 cut(s) | 445, 612 |
| NlaIII | CATG | 3 cut(s) | 79, 680, 721 |
| NlaIV | GGNNCC | 1 cut(s) | 243 |
| NmuCI | GTSAC | 2 cut(s) | 85, 576 |
| NspI | RCATGY | 1 cut(s) | 79 |
| PaeR7I | CTCGAG | 1 cut(s) | 278 |
| PciI | ACATGT | 1 cut(s) | 75 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 156, 297, 623 |
| PdmI | GAANNNNTTC | 1 cut(s) | 476 |
| PfeI | GAWTC | 1 cut(s) | 472 |
| PkrI | GCNGC | 4 cut(s) | 328, 408, 544, 625 |
| PleI | GAGTC | 2 cut(s) | 585, 609 |
| PpsI | GAGTC | 2 cut(s) | 585, 609 |
| Ppu21I | YACGTR | 1 cut(s) | 490 |
| PscI | ACATGT | 1 cut(s) | 75 |
| PshAI | GACNNNNGTC | 1 cut(s) | 725 |
| Psp124BI | GAGCTC | 1 cut(s) | 429 |
| Psp1406I | AACGTT | 1 cut(s) | 333 |
| Psp6I | CCWGG | 1 cut(s) | 384 |
| PspGI | CCWGG | 1 cut(s) | 384 |
| PspN4I | GGNNCC | 1 cut(s) | 243 |
| PspPI | GGNCC | 2 cut(s) | 673, 727 |
| PspXI | VCTCGAGB | 1 cut(s) | 278 |
| PsuI | RGATCY | 2 cut(s) | 445, 612 |
| PvuII | CAGCTG | 1 cut(s) | 750 |
| SacI | GAGCTC | 1 cut(s) | 429 |
| SaqAI | TTAA | 3 cut(s) | 36, 288, 440 |
| SatI | GCNGC | 4 cut(s) | 327, 407, 543, 624 |
| Sau3AI | GATC | 2 cut(s) | 445, 612 |
| Sau96I | GGNCC | 2 cut(s) | 673, 727 |
| SchI | GAGTC | 2 cut(s) | 585, 610 |
| ScrFI | CCNGG | 1 cut(s) | 386 |
| SduI | GDGCHC | 3 cut(s) | 75, 316, 429 |
| SfaNI | GCATC | 1 cut(s) | 652 |
| Sfr274I | CTCGAG | 1 cut(s) | 278 |
| SinI | GGWCC | 1 cut(s) | 727 |
| SlaI | CTCGAG | 1 cut(s) | 278 |
| SmlI | CTYRAG | 1 cut(s) | 278 |
| SmoI | CTYRAG | 1 cut(s) | 278 |
| Sse9I | AATT | 3 cut(s) | 33, 128, 551 |
| SsiI | CCGC | 4 cut(s) | 308, 327, 623, 730 |
| SspMI | CTAG | 3 cut(s) | 56, 176, 573 |
| SstI | GAGCTC | 1 cut(s) | 429 |
| StyD4I | CCNGG | 1 cut(s) | 384 |
| TaaI | ACNGT | 3 cut(s) | 401, 512, 642 |
| TaiI | ACGT | 5 cut(s) | 150, 162, 336, 492, 684 |
| TaqI | TCGA | 4 cut(s) | 150, 279, 396, 617 |
| TasI | AATT | 3 cut(s) | 33, 128, 551 |
| TauI | GCSGC | 2 cut(s) | 329, 626 |
| TfiI | GAWTC | 1 cut(s) | 472 |
| Tru1I | TTAA | 3 cut(s) | 36, 288, 440 |
| Tru9I | TTAA | 3 cut(s) | 36, 288, 440 |
| TseFI | GTSAC | 2 cut(s) | 85, 576 |
| TseI | GCWGC | 2 cut(s) | 406, 542 |
| Tsp45I | GTSAC | 2 cut(s) | 85, 576 |
| TspDTI | ATGAA | 3 cut(s) | 224, 530, 623 |
| VpaK11BI | GGWCC | 1 cut(s) | 727 |
| XapI | RAATTY | 1 cut(s) | 128 |
| XceI | RCATGY | 1 cut(s) | 79 |
| XhoI | CTCGAG | 1 cut(s) | 278 |
| XmiI | GTMKAC | 1 cut(s) | 653 |
| XmnI | GAANNNNTTC | 1 cut(s) | 476 |
| XspI | CTAG | 3 cut(s) | 56, 176, 573 |
| ZraI | GACGTC | 1 cut(s) | 148 |