Uncharacterized protein K02A2.6-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1127 |
| AccB1I | GGYRCC | 1 cut(s) | 670 |
| AccI | GTMKAC | 3 cut(s) | 993, 1121, 1151 |
| AccII | CGCG | 3 cut(s) | 506, 865, 1055 |
| AclWI | GGATC | 5 cut(s) | 16, 272, 633, 698, 937 |
| AcuI | CTGAAG | 2 cut(s) | 348, 641 |
| AcyI | GRCGYC | 1 cut(s) | 1124 |
| AfaI | GTAC | 2 cut(s) | 5, 307 |
| AfeI | AGCGCT | 1 cut(s) | 14 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 213, 244, 719, 901 |
| AgsI | TTSAA | 7 cut(s) | 40, 130, 375, 799, 1039, 1048, 1191 |
| AjiI | CACGTC | 2 cut(s) | 466, 739 |
| AjnI | CCWGG | 2 cut(s) | 226, 348 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 428, 460 |
| AluBI | AGCT | 6 cut(s) | 344, 415, 553, 605, 619, 1185 |
| AluI | AGCT | 6 cut(s) | 344, 415, 553, 605, 619, 1185 |
| Alw21I | GWGCWC | 2 cut(s) | 174, 1187 |
| AlwI | GGATC | 5 cut(s) | 16, 272, 633, 698, 937 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 317 |
| Aor51HI | AGCGCT | 1 cut(s) | 14 |
| AoxI | GGCC | 1 cut(s) | 529 |
| ApeKI | GCWGC | 6 cut(s) | 248, 344, 550, 683, 686, 981 |
| AspLEI | GCGC | 1 cut(s) | 15 |
| AspS9I | GGNCC | 2 cut(s) | 530, 697 |
| AsuHPI | GGTGA | 5 cut(s) | 79, 421, 424, 526, 761 |
| AvaII | GGWCC | 1 cut(s) | 697 |
| BanI | GGYRCC | 1 cut(s) | 670 |
| BanII | GRGCYC | 1 cut(s) | 1187 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 203, 235, 554, 586 |
| BbsI | GAAGAC | 1 cut(s) | 1055 |
| Bbv12I | GWGCWC | 2 cut(s) | 174, 1187 |
| BbvI | GCAGC | 6 cut(s) | 235, 331, 562, 695, 698, 993 |
| BccI | CCATC | 5 cut(s) | 181, 267, 857, 1139, 1140 |
| BciT130I | CCWGG | 2 cut(s) | 228, 350 |
| BfaI | CTAG | 5 cut(s) | 303, 545, 701, 896, 1247 |
| BfmI | CTRYAG | 1 cut(s) | 961 |
| BfoI | RGCGCY | 1 cut(s) | 16 |
| Bme1390I | CCNGG | 2 cut(s) | 228, 350 |
| Bme18I | GGWCC | 1 cut(s) | 697 |
| BmgBI | CACGTC | 2 cut(s) | 466, 739 |
| BmgT120I | GGNCC | 2 cut(s) | 530, 697 |
| BmiI | GGNNCC | 1 cut(s) | 672 |
| BmrFI | CCNGG | 2 cut(s) | 228, 350 |
| BmrI | ACTGGG | 1 cut(s) | 369 |
| BmsI | GCATC | 1 cut(s) | 732 |
| BmuI | ACTGGG | 1 cut(s) | 369 |
| BpiI | GAAGAC | 1 cut(s) | 1055 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1169, 1201 |
| BpmI | CTGGAG | 1 cut(s) | 1190 |
| Bpu10I | CCTNAGC | 3 cut(s) | 179, 554, 789 |
| BpuEI | CTTGAG | 2 cut(s) | 401, 873 |
| BsaHI | GRCGYC | 1 cut(s) | 1124 |
| BsaJI | CCNNGG | 6 cut(s) | 135, 226, 227, 348, 1019, 1236 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 801, 831, 1000, 1030 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 213, 244, 719, 901 |
| Bse118I | RCCGGY | 2 cut(s) | 406, 719 |
| Bse1I | ACTGG | 4 cut(s) | 69, 267, 364, 1115 |
| BseBI | CCWGG | 2 cut(s) | 228, 350 |
| BseDI | CCNNGG | 6 cut(s) | 135, 226, 227, 348, 1019, 1236 |
| BseGI | GGATG | 3 cut(s) | 222, 908, 956 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 213, 244, 719, 901 |
| BseMII | CTCAG | 1 cut(s) | 193 |
| BseNI | ACTGG | 4 cut(s) | 69, 267, 364, 1115 |
| BseXI | GCAGC | 6 cut(s) | 235, 331, 562, 695, 698, 993 |
| Bsh1236I | CGCG | 3 cut(s) | 506, 865, 1055 |
| BshFI | GGCC | 1 cut(s) | 531 |
| BshNI | GGYRCC | 1 cut(s) | 670 |
| BsiHKAI | GWGCWC | 2 cut(s) | 174, 1187 |
| BsiSI | CCGG | 3 cut(s) | 53, 407, 720 |
| BslFI | GGGAC | 2 cut(s) | 229, 1037 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 213, 244, 719, 901 |
| BsmFI | GGGAC | 2 cut(s) | 229, 1037 |
| BsnI | GGCC | 1 cut(s) | 531 |
| Bsp1286I | GDGCHC | 2 cut(s) | 174, 1187 |
| Bsp143I | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| BspANI | GGCC | 1 cut(s) | 531 |
| BspCNI | CTCAG | 1 cut(s) | 192 |
| BspFNI | CGCG | 3 cut(s) | 506, 865, 1055 |
| BspHI | TCATGA | 1 cut(s) | 819 |
| BspLI | GGNNCC | 1 cut(s) | 672 |
| BspPI | GGATC | 5 cut(s) | 16, 272, 633, 698, 937 |
| BspQI | GCTCTTC | 2 cut(s) | 1059, 1192 |
| BspT107I | GGYRCC | 1 cut(s) | 670 |
| BsrFI | RCCGGY | 2 cut(s) | 406, 719 |
| BsrI | ACTGG | 4 cut(s) | 69, 267, 364, 1115 |
| BssAI | RCCGGY | 2 cut(s) | 406, 719 |
| BssECI | CCNNGG | 6 cut(s) | 135, 226, 227, 348, 1019, 1236 |
| BssMI | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| BssNAI | GTATAC | 2 cut(s) | 994, 1152 |
| BssNI | GRCGYC | 1 cut(s) | 1124 |
| BssT1I | CCWWGG | 2 cut(s) | 1019, 1236 |
| Bst1107I | GTATAC | 2 cut(s) | 994, 1152 |
| Bst2UI | CCWGG | 2 cut(s) | 228, 350 |
| Bst4CI | ACNGT | 5 cut(s) | 396, 653, 919, 948, 998 |
| Bst6I | CTCTTC | 3 cut(s) | 1059, 1190, 1192 |
| BstACI | GRCGYC | 1 cut(s) | 1124 |
| BstC8I | GCNNGC | 1 cut(s) | 863 |
| BstDEI | CTNAG | 4 cut(s) | 88, 179, 554, 789 |
| BstDSI | CCRYGG | 1 cut(s) | 135 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 899 |
| BstF5I | GGATG | 3 cut(s) | 222, 908, 956 |
| BstFNI | CGCG | 3 cut(s) | 506, 865, 1055 |
| BstH2I | RGCGCY | 1 cut(s) | 16 |
| BstHHI | GCGC | 1 cut(s) | 15 |
| BstKTI | GATC | 7 cut(s) | 24, 280, 501, 628, 706, 760, 932 |
| BstMBI | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 412, 867 |
| BstNI | CCWGG | 2 cut(s) | 228, 350 |
| BstNSI | RCATGY | 1 cut(s) | 127 |
| BstSCI | CCNGG | 2 cut(s) | 226, 348 |
| BstSFI | CTRYAG | 1 cut(s) | 961 |
| BstUI | CGCG | 3 cut(s) | 506, 865, 1055 |
| BstV1I | GCAGC | 6 cut(s) | 235, 331, 562, 695, 698, 993 |
| BstV2I | GAAGAC | 1 cut(s) | 1055 |
| BstX2I | RGATCY | 3 cut(s) | 21, 703, 929 |
| BstYI | RGATCY | 3 cut(s) | 21, 703, 929 |
| BstZ17I | GTATAC | 2 cut(s) | 994, 1152 |
| BsuRI | GGCC | 1 cut(s) | 531 |
| BtgI | CCRYGG | 1 cut(s) | 135 |
| BtrI | CACGTC | 2 cut(s) | 466, 739 |
| BtsCI | GGATG | 3 cut(s) | 222, 908, 956 |
| BtsIMutI | CAGTG | 3 cut(s) | 357, 1003, 1122 |
| Cac8I | GCNNGC | 1 cut(s) | 863 |
| CaiI | CAGNNNCTG | 1 cut(s) | 317 |
| CciI | TCATGA | 1 cut(s) | 819 |
| CfoI | GCGC | 1 cut(s) | 15 |
| Cfr10I | RCCGGY | 2 cut(s) | 406, 719 |
| Cfr13I | GGNCC | 2 cut(s) | 530, 697 |
| CseI | GACGC | 1 cut(s) | 200 |
| Csp6I | GTAC | 2 cut(s) | 4, 306 |
| CviAII | CATG | 8 cut(s) | 116, 124, 479, 730, 744, 782, 820, 978 |
| CviQI | GTAC | 2 cut(s) | 4, 306 |
| DdeI | CTNAG | 4 cut(s) | 88, 179, 554, 789 |
| DpnI | GATC | 7 cut(s) | 23, 279, 500, 627, 705, 759, 931 |
| DpnII | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| Eam1104I | CTCTTC | 3 cut(s) | 1059, 1190, 1192 |
| EarI | CTCTTC | 3 cut(s) | 1059, 1190, 1192 |
| EciI | GGCGGA | 1 cut(s) | 1101 |
| Ecl136II | GAGCTC | 1 cut(s) | 1185 |
| Eco130I | CCWWGG | 2 cut(s) | 1019, 1236 |
| Eco24I | GRGCYC | 1 cut(s) | 1187 |
| Eco47I | GGWCC | 1 cut(s) | 697 |
| Eco47III | AGCGCT | 1 cut(s) | 14 |
| Eco53kI | GAGCTC | 1 cut(s) | 1185 |
| Eco57I | CTGAAG | 2 cut(s) | 348, 641 |
| EcoICRI | GAGCTC | 1 cut(s) | 1185 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 899 |
| EcoRII | CCWGG | 2 cut(s) | 226, 348 |
| EcoT14I | CCWWGG | 2 cut(s) | 1019, 1236 |
| EcoT38I | GRGCYC | 1 cut(s) | 1187 |
| ErhI | CCWWGG | 2 cut(s) | 1019, 1236 |
| FaeI | CATG | 8 cut(s) | 119, 127, 482, 733, 747, 785, 823, 981 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 442, 474, 1220, 1252 |
| FaqI | GGGAC | 2 cut(s) | 229, 1037 |
| FatI | CATG | 8 cut(s) | 115, 123, 478, 729, 743, 781, 819, 977 |
| FauI | CCCGC | 1 cut(s) | 819 |
| FblI | GTMKAC | 3 cut(s) | 993, 1121, 1151 |
| FokI | GGATG | 3 cut(s) | 209, 895, 943 |
| FriOI | GRGCYC | 1 cut(s) | 1187 |
| FspBI | CTAG | 5 cut(s) | 303, 545, 701, 896, 1247 |
| GlaI | GCGC | 1 cut(s) | 14 |
| GsuI | CTGGAG | 1 cut(s) | 1190 |
| HaeII | RGCGCY | 1 cut(s) | 16 |
| HaeIII | GGCC | 1 cut(s) | 531 |
| HapII | CCGG | 3 cut(s) | 53, 407, 720 |
| HgaI | GACGC | 1 cut(s) | 200 |
| HhaI | GCGC | 1 cut(s) | 15 |
| Hin1I | GRCGYC | 1 cut(s) | 1124 |
| Hin1II | CATG | 8 cut(s) | 119, 127, 482, 733, 747, 785, 823, 981 |
| Hin6I | GCGC | 1 cut(s) | 13 |
| HinP1I | GCGC | 1 cut(s) | 13 |
| HincII | GTYRAC | 1 cut(s) | 1122 |
| HindII | GTYRAC | 1 cut(s) | 1122 |
| HinfI | GANTC | 8 cut(s) | 46, 299, 323, 592, 766, 830, 838, 1025 |
| HpaII | CCGG | 3 cut(s) | 53, 407, 720 |
| HphI | GGTGA | 5 cut(s) | 79, 421, 424, 526, 761 |
| Hpy166II | GTNNAC | 8 cut(s) | 447, 469, 697, 736, 845, 994, 1122, 1152 |
| Hpy188I | TCNGA | 3 cut(s) | 21, 328, 1006 |
| Hpy188III | TCNNGA | 3 cut(s) | 25, 776, 820 |
| Hpy8I | GTNNAC | 8 cut(s) | 447, 469, 697, 736, 845, 994, 1122, 1152 |
| Hpy99I | CGWCG | 2 cut(s) | 467, 1126 |
| HpyAV | CCTTC | 6 cut(s) | 17, 205, 616, 718, 1133, 1156 |
| HpyCH4III | ACNGT | 5 cut(s) | 396, 653, 919, 948, 998 |
| HpyCH4IV | ACGT | 4 cut(s) | 42, 465, 738, 1124 |
| HpyCH4V | TGCA | 6 cut(s) | 251, 399, 478, 661, 870, 981 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 412, 867 |
| HpyF3I | CTNAG | 4 cut(s) | 88, 179, 554, 789 |
| HpySE526I | ACGT | 4 cut(s) | 42, 465, 738, 1124 |
| Hsp92I | GRCGYC | 1 cut(s) | 1124 |
| Hsp92II | CATG | 8 cut(s) | 119, 127, 482, 733, 747, 785, 823, 981 |
| HspAI | GCGC | 1 cut(s) | 13 |
| Kzo9I | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| LguI | GCTCTTC | 2 cut(s) | 1059, 1192 |
| Lsp1109I | GCAGC | 6 cut(s) | 235, 331, 562, 695, 698, 993 |
| LweI | GCATC | 1 cut(s) | 732 |
| MaeI | CTAG | 5 cut(s) | 303, 545, 701, 896, 1247 |
| MaeII | ACGT | 4 cut(s) | 42, 465, 738, 1124 |
| MaeIII | GTNAC | 1 cut(s) | 881 |
| MalI | GATC | 7 cut(s) | 23, 279, 500, 627, 705, 759, 931 |
| MboI | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| MflI | RGATCY | 3 cut(s) | 21, 703, 929 |
| MhlI | GDGCHC | 2 cut(s) | 174, 1187 |
| MluCI | AATT | 2 cut(s) | 71, 130 |
| MlyI | GAGTC | 3 cut(s) | 332, 832, 1019 |
| MmeI | TCCRAC | 2 cut(s) | 787, 997 |
| MnlI | CCTC | 8 cut(s) | 217, 289, 307, 661, 895, 1050, 1138, 1206 |
| MseI | TTAA | 4 cut(s) | 615, 644, 1094, 1252 |
| MslI | CAYNNNNRTG | 1 cut(s) | 728 |
| MspA1I | CMGCKG | 1 cut(s) | 605 |
| MspI | CCGG | 3 cut(s) | 53, 407, 720 |
| MspR9I | CCNGG | 2 cut(s) | 228, 350 |
| MvaI | CCWGG | 2 cut(s) | 228, 350 |
| MvnI | CGCG | 3 cut(s) | 506, 865, 1055 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 412, 867 |
| NdeII | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| NlaIII | CATG | 8 cut(s) | 119, 127, 482, 733, 747, 785, 823, 981 |
| NlaIV | GGNNCC | 1 cut(s) | 672 |
| NmuCI | GTSAC | 1 cut(s) | 881 |
| NspI | RCATGY | 1 cut(s) | 127 |
| PagI | TCATGA | 1 cut(s) | 819 |
| PasI | CCCWGGG | 1 cut(s) | 227 |
| PciSI | GCTCTTC | 2 cut(s) | 1059, 1192 |
| PfeI | GAWTC | 5 cut(s) | 46, 299, 592, 766, 830 |
| PleI | GAGTC | 3 cut(s) | 331, 832, 1019 |
| PpsI | GAGTC | 3 cut(s) | 331, 832, 1019 |
| Psp124BI | GAGCTC | 1 cut(s) | 1187 |
| Psp6I | CCWGG | 2 cut(s) | 226, 348 |
| PspGI | CCWGG | 2 cut(s) | 226, 348 |
| PspN4I | GGNNCC | 1 cut(s) | 672 |
| PspPI | GGNCC | 2 cut(s) | 530, 697 |
| PstNI | CAGNNNCTG | 1 cut(s) | 317 |
| PsuI | RGATCY | 3 cut(s) | 21, 703, 929 |
| PvuII | CAGCTG | 1 cut(s) | 605 |
| RsaI | GTAC | 2 cut(s) | 5, 307 |
| RsaNI | GTAC | 2 cut(s) | 4, 306 |
| RseI | CAYNNNNRTG | 1 cut(s) | 728 |
| SacI | GAGCTC | 1 cut(s) | 1187 |
| SalI | GTCGAC | 1 cut(s) | 1120 |
| SapI | GCTCTTC | 2 cut(s) | 1059, 1192 |
| SaqAI | TTAA | 4 cut(s) | 615, 644, 1094, 1252 |
| Sau3AI | GATC | 7 cut(s) | 21, 277, 498, 625, 703, 757, 929 |
| Sau96I | GGNCC | 2 cut(s) | 530, 697 |
| SchI | GAGTC | 3 cut(s) | 332, 832, 1019 |
| ScrFI | CCNGG | 2 cut(s) | 228, 350 |
| SduI | GDGCHC | 2 cut(s) | 174, 1187 |
| SfaNI | GCATC | 1 cut(s) | 732 |
| SfcI | CTRYAG | 1 cut(s) | 961 |
| SinI | GGWCC | 1 cut(s) | 697 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 728 |
| SmlI | CTYRAG | 2 cut(s) | 416, 888 |
| SmoI | CTYRAG | 2 cut(s) | 416, 888 |
| Sse9I | AATT | 2 cut(s) | 71, 130 |
| SspMI | CTAG | 5 cut(s) | 303, 545, 701, 896, 1247 |
| SstI | GAGCTC | 1 cut(s) | 1187 |
| StyD4I | CCNGG | 2 cut(s) | 226, 348 |
| StyI | CCWWGG | 2 cut(s) | 1019, 1236 |
| TaaI | ACNGT | 5 cut(s) | 396, 653, 919, 948, 998 |
| TaiI | ACGT | 4 cut(s) | 45, 468, 741, 1127 |
| TaqI | TCGA | 4 cut(s) | 168, 209, 489, 1121 |
| TasI | AATT | 2 cut(s) | 71, 130 |
| TatI | WGTACW | 1 cut(s) | 305 |
| TauI | GCSGC | 4 cut(s) | 506, 788, 940, 1084 |
| TfiI | GAWTC | 5 cut(s) | 46, 299, 592, 766, 830 |
| Tru1I | TTAA | 4 cut(s) | 615, 644, 1094, 1252 |
| Tru9I | TTAA | 4 cut(s) | 615, 644, 1094, 1252 |
| TscAI | CASTG | 3 cut(s) | 364, 1003, 1122 |
| TseFI | GTSAC | 1 cut(s) | 881 |
| TseI | GCWGC | 6 cut(s) | 248, 344, 550, 683, 686, 981 |
| Tsp45I | GTSAC | 1 cut(s) | 881 |
| TspDTI | ATGAA | 4 cut(s) | 212, 495, 507, 836 |
| TspRI | CASTG | 3 cut(s) | 364, 1003, 1122 |
| VpaK11BI | GGWCC | 1 cut(s) | 697 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 899 |
| XceI | RCATGY | 1 cut(s) | 127 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1214 |
| XmiI | GTMKAC | 3 cut(s) | 993, 1121, 1151 |
| XspI | CTAG | 5 cut(s) | 303, 545, 701, 896, 1247 |
| ZraI | GACGTC | 1 cut(s) | 1125 |