Reverse transcriptase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 231 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 995 |
| AccBSI | CCGCTC | 1 cut(s) | 493 |
| AccI | GTMKAC | 3 cut(s) | 66, 565, 935 |
| AccII | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| AclWI | GGATC | 2 cut(s) | 83, 1037 |
| AcoI | YGGCCR | 3 cut(s) | 753, 1158, 1516 |
| AcsI | RAATTY | 2 cut(s) | 514, 1019 |
| AfaI | GTAC | 3 cut(s) | 12, 867, 1523 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 556, 995, 1279, 1307, 1375 |
| AgsI | TTSAA | 6 cut(s) | 272, 299, 509, 1018, 1193, 1504 |
| AjiI | CACGTC | 2 cut(s) | 933, 1322 |
| AjnI | CCWGG | 7 cut(s) | 89, 417, 825, 1102, 1177, 1300, 1306 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 788, 820 |
| AluBI | AGCT | 6 cut(s) | 99, 607, 676, 848, 1099, 1577 |
| AluI | AGCT | 6 cut(s) | 99, 607, 676, 848, 1099, 1577 |
| Alw21I | GWGCWC | 1 cut(s) | 1101 |
| Alw26I | GTCTC | 1 cut(s) | 876 |
| AlwI | GGATC | 2 cut(s) | 83, 1037 |
| Ama87I | CYCGRG | 2 cut(s) | 879, 1209 |
| ApaI | GGGCCC | 1 cut(s) | 1366 |
| ApeKI | GCWGC | 4 cut(s) | 521, 760, 1286, 1577 |
| ApoI | RAATTY | 2 cut(s) | 514, 1019 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 613, 645 |
| AspA2I | CCTAGG | 1 cut(s) | 1358 |
| AspLEI | GCGC | 1 cut(s) | 434 |
| AspS9I | GGNCC | 8 cut(s) | 21, 559, 732, 1270, 1304, 1362, 1363, 1388 |
| AsuC2I | CCSGG | 4 cut(s) | 73, 105, 1210, 1211 |
| AsuHPI | GGTGA | 3 cut(s) | 406, 973, 980 |
| AvaI | CYCGRG | 2 cut(s) | 879, 1209 |
| AvaII | GGWCC | 3 cut(s) | 21, 559, 732 |
| AvrII | CCTAGG | 1 cut(s) | 1358 |
| BaeGI | GKGCMC | 4 cut(s) | 236, 616, 1136, 1366 |
| BalI | TGGCCA | 2 cut(s) | 755, 1160 |
| BanI | GGYRCC | 1 cut(s) | 231 |
| BanII | GRGCYC | 2 cut(s) | 1101, 1366 |
| Bbv12I | GWGCWC | 1 cut(s) | 1101 |
| BbvI | GCAGC | 4 cut(s) | 508, 772, 1273, 1564 |
| BccI | CCATC | 7 cut(s) | 328, 532, 632, 722, 830, 1067, 1281 |
| BceAI | ACGGC | 2 cut(s) | 584, 1284 |
| BciT130I | CCWGG | 7 cut(s) | 91, 419, 827, 1104, 1179, 1302, 1308 |
| BclI | TGATCA | 2 cut(s) | 141, 1123 |
| BcnI | CCSGG | 4 cut(s) | 73, 105, 1210, 1211 |
| BcoDI | GTCTC | 1 cut(s) | 876 |
| BfaI | CTAG | 5 cut(s) | 96, 408, 608, 1359, 1497 |
| BfmI | CTRYAG | 1 cut(s) | 924 |
| BlnI | CCTAGG | 1 cut(s) | 1358 |
| Bme18I | GGWCC | 3 cut(s) | 21, 559, 732 |
| BmeT110I | CYCGRG | 2 cut(s) | 879, 1209 |
| BmgBI | CACGTC | 2 cut(s) | 933, 1322 |
| BmgT120I | GGNCC | 8 cut(s) | 21, 559, 732, 1270, 1304, 1362, 1363, 1388 |
| BmiI | GGNNCC | 5 cut(s) | 22, 233, 285, 733, 1364 |
| BmrI | ACTGGG | 2 cut(s) | 23, 782 |
| BmsI | GCATC | 2 cut(s) | 420, 1242 |
| BmuI | ACTGGG | 2 cut(s) | 23, 782 |
| BpuEI | CTTGAG | 2 cut(s) | 623, 1526 |
| BpuMI | CCSGG | 4 cut(s) | 73, 105, 1210, 1211 |
| Bsa29I | ATCGAT | 2 cut(s) | 158, 323 |
| BsaWI | WCCGGW | 1 cut(s) | 206 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 99, 129, 1120, 1150 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 556, 995, 1279, 1307, 1375 |
| Bse118I | RCCGGY | 1 cut(s) | 991 |
| Bse1I | ACTGG | 3 cut(s) | 18, 777, 1519 |
| Bse3DI | GCAATG | 2 cut(s) | 696, 1517 |
| BseBI | CCWGG | 7 cut(s) | 91, 419, 827, 1104, 1179, 1302, 1308 |
| BseCI | ATCGAT | 2 cut(s) | 158, 323 |
| BseGI | GGATG | 4 cut(s) | 177, 643, 822, 1171 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 556, 995, 1279, 1307, 1375 |
| BseMI | GCAATG | 2 cut(s) | 696, 1517 |
| BseNI | ACTGG | 3 cut(s) | 18, 777, 1519 |
| BseRI | GAGGAG | 1 cut(s) | 1340 |
| BseSI | GKGCMC | 4 cut(s) | 236, 616, 1136, 1366 |
| BseXI | GCAGC | 4 cut(s) | 508, 772, 1273, 1564 |
| BseYI | CCCAGC | 1 cut(s) | 381 |
| BsgI | GTGCAG | 1 cut(s) | 1272 |
| Bsh1236I | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| Bsh1285I | CGRYCG | 1 cut(s) | 207 |
| BshNI | GGYRCC | 1 cut(s) | 231 |
| BshVI | ATCGAT | 2 cut(s) | 158, 323 |
| BsiEI | CGRYCG | 1 cut(s) | 207 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1101 |
| BsiHKCI | CYCGRG | 2 cut(s) | 879, 1209 |
| BsiSI | CCGG | 5 cut(s) | 72, 104, 207, 992, 1210 |
| BslFI | GGGAC | 2 cut(s) | 34, 789 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 556, 995, 1279, 1307, 1375 |
| BsmAI | GTCTC | 1 cut(s) | 876 |
| BsmFI | GGGAC | 2 cut(s) | 34, 789 |
| BsmI | GAATGC | 1 cut(s) | 880 |
| BsoBI | CYCGRG | 2 cut(s) | 879, 1209 |
| Bsp120I | GGGCCC | 1 cut(s) | 1362 |
| Bsp1286I | GDGCHC | 5 cut(s) | 236, 616, 1101, 1136, 1366 |
| Bsp143I | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| Bsp68I | TCGCGA | 1 cut(s) | 743 |
| BspDI | ATCGAT | 2 cut(s) | 158, 323 |
| BspFNI | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| BspLI | GGNNCC | 5 cut(s) | 22, 233, 285, 733, 1364 |
| BspPI | GGATC | 2 cut(s) | 83, 1037 |
| BspQI | GCTCTTC | 2 cut(s) | 483, 666 |
| BspT107I | GGYRCC | 1 cut(s) | 231 |
| BsrBI | CCGCTC | 1 cut(s) | 493 |
| BsrDI | GCAATG | 2 cut(s) | 696, 1517 |
| BsrFI | RCCGGY | 1 cut(s) | 991 |
| BsrI | ACTGG | 3 cut(s) | 18, 777, 1519 |
| BssAI | RCCGGY | 1 cut(s) | 991 |
| BssMI | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| BssT1I | CCWWGG | 3 cut(s) | 783, 1161, 1358 |
| Bst2UI | CCWGG | 7 cut(s) | 91, 419, 827, 1104, 1179, 1302, 1308 |
| Bst4CI | ACNGT | 4 cut(s) | 38, 563, 1034, 1282 |
| Bst6I | CTCTTC | 3 cut(s) | 483, 666, 1302 |
| BstC8I | GCNNGC | 6 cut(s) | 101, 397, 430, 688, 765, 1413 |
| BstDSI | CCRYGG | 1 cut(s) | 24 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 554, 1305 |
| BstF5I | GGATG | 4 cut(s) | 177, 643, 822, 1171 |
| BstFNI | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| BstHHI | GCGC | 1 cut(s) | 434 |
| BstKTI | GATC | 7 cut(s) | 78, 144, 151, 158, 1045, 1126, 1149 |
| BstMAI | GTCTC | 1 cut(s) | 876 |
| BstMBI | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| BstMCI | CGRYCG | 1 cut(s) | 207 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 431, 1261, 1545 |
| BstNI | CCWGG | 7 cut(s) | 91, 419, 827, 1104, 1179, 1302, 1308 |
| BstSFI | CTRYAG | 1 cut(s) | 924 |
| BstSLI | GKGCMC | 4 cut(s) | 236, 616, 1136, 1366 |
| BstUI | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| BstV1I | GCAGC | 4 cut(s) | 508, 772, 1273, 1564 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1373 |
| Bsu15I | ATCGAT | 2 cut(s) | 158, 323 |
| BsuTUI | ATCGAT | 2 cut(s) | 158, 323 |
| BtgI | CCRYGG | 1 cut(s) | 24 |
| BtgZI | GCGATG | 2 cut(s) | 694, 1265 |
| BtrI | CACGTC | 2 cut(s) | 933, 1322 |
| BtsCI | GGATG | 4 cut(s) | 177, 643, 822, 1171 |
| BtsI | GCAGTG | 1 cut(s) | 1149 |
| BtsIMutI | CAGTG | 2 cut(s) | 151, 1149 |
| BtuMI | TCGCGA | 1 cut(s) | 743 |
| Cac8I | GCNNGC | 6 cut(s) | 101, 397, 430, 688, 765, 1413 |
| CfoI | GCGC | 1 cut(s) | 434 |
| Cfr10I | RCCGGY | 1 cut(s) | 991 |
| Cfr13I | GGNCC | 8 cut(s) | 21, 559, 732, 1270, 1304, 1362, 1363, 1388 |
| Cfr42I | CCGCGG | 1 cut(s) | 27 |
| Cfr9I | CCCGGG | 1 cut(s) | 1209 |
| ClaI | ATCGAT | 2 cut(s) | 158, 323 |
| Csp6I | GTAC | 3 cut(s) | 11, 866, 1522 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 208, 243 |
| CviAII | CATG | 6 cut(s) | 643, 668, 757, 1222, 1247, 1408 |
| CviQI | GTAC | 3 cut(s) | 11, 866, 1522 |
| DpnI | GATC | 7 cut(s) | 77, 143, 150, 157, 1044, 1125, 1148 |
| DpnII | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| EaeI | YGGCCR | 3 cut(s) | 753, 1158, 1516 |
| Eam1104I | CTCTTC | 3 cut(s) | 483, 666, 1302 |
| EarI | CTCTTC | 3 cut(s) | 483, 666, 1302 |
| Ecl136II | GAGCTC | 1 cut(s) | 1099 |
| Eco130I | CCWWGG | 3 cut(s) | 783, 1161, 1358 |
| Eco24I | GRGCYC | 2 cut(s) | 1101, 1366 |
| Eco47I | GGWCC | 3 cut(s) | 21, 559, 732 |
| Eco53kI | GAGCTC | 1 cut(s) | 1099 |
| Eco88I | CYCGRG | 2 cut(s) | 879, 1209 |
| EcoICRI | GAGCTC | 1 cut(s) | 1099 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 554, 1305 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1362, 1388 |
| EcoRI | GAATTC | 1 cut(s) | 514 |
| EcoRII | CCWGG | 7 cut(s) | 89, 417, 825, 1102, 1177, 1300, 1306 |
| EcoT14I | CCWWGG | 3 cut(s) | 783, 1161, 1358 |
| EcoT38I | GRGCYC | 2 cut(s) | 1101, 1366 |
| ErhI | CCWWGG | 3 cut(s) | 783, 1161, 1358 |
| FaeI | CATG | 6 cut(s) | 646, 671, 760, 1225, 1250, 1411 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 172, 204, 480, 512 |
| FaqI | GGGAC | 2 cut(s) | 34, 789 |
| FatI | CATG | 6 cut(s) | 642, 667, 756, 1221, 1246, 1407 |
| FauNDI | CATATG | 1 cut(s) | 913 |
| FbaI | TGATCA | 2 cut(s) | 141, 1123 |
| FblI | GTMKAC | 3 cut(s) | 66, 565, 935 |
| FokI | GGATG | 4 cut(s) | 184, 650, 809, 1178 |
| FriOI | GRGCYC | 2 cut(s) | 1101, 1366 |
| FspBI | CTAG | 5 cut(s) | 96, 408, 608, 1359, 1497 |
| GlaI | GCGC | 1 cut(s) | 433 |
| GsaI | CCCAGC | 1 cut(s) | 385 |
| HapII | CCGG | 5 cut(s) | 72, 104, 207, 992, 1210 |
| HhaI | GCGC | 1 cut(s) | 434 |
| Hin1II | CATG | 6 cut(s) | 646, 671, 760, 1225, 1250, 1411 |
| Hin6I | GCGC | 1 cut(s) | 432 |
| HinP1I | GCGC | 1 cut(s) | 432 |
| HincII | GTYRAC | 3 cut(s) | 67, 336, 566 |
| HindII | GTYRAC | 3 cut(s) | 67, 336, 566 |
| HinfI | GANTC | 4 cut(s) | 68, 160, 295, 812 |
| HpaII | CCGG | 5 cut(s) | 72, 104, 207, 992, 1210 |
| HphI | GGTGA | 3 cut(s) | 406, 973, 980 |
| Hpy166II | GTNNAC | 6 cut(s) | 67, 336, 566, 582, 936, 1090 |
| Hpy188I | TCNGA | 3 cut(s) | 795, 1128, 1278 |
| Hpy188III | TCNNGA | 8 cut(s) | 118, 470, 595, 742, 881, 1144, 1461, 1572 |
| Hpy8I | GTNNAC | 6 cut(s) | 67, 336, 566, 582, 936, 1090 |
| Hpy99I | CGWCG | 3 cut(s) | 567, 570, 1323 |
| HpyAV | CCTTC | 4 cut(s) | 266, 515, 1302, 1379 |
| HpyCH4III | ACNGT | 4 cut(s) | 38, 563, 1034, 1282 |
| HpyCH4IV | ACGT | 3 cut(s) | 404, 932, 1321 |
| HpyCH4V | TGCA | 9 cut(s) | 524, 554, 658, 701, 760, 1264, 1289, 1510, 1548 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 431, 1261, 1545 |
| HpySE526I | ACGT | 3 cut(s) | 404, 932, 1321 |
| Hsp92II | CATG | 6 cut(s) | 646, 671, 760, 1225, 1250, 1411 |
| HspAI | GCGC | 1 cut(s) | 432 |
| Ksp22I | TGATCA | 2 cut(s) | 141, 1123 |
| KspI | CCGCGG | 1 cut(s) | 27 |
| Kzo9I | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| LguI | GCTCTTC | 2 cut(s) | 483, 666 |
| LmnI | GCTCC | 3 cut(s) | 283, 1104, 1574 |
| Lsp1109I | GCAGC | 4 cut(s) | 508, 772, 1273, 1564 |
| LweI | GCATC | 2 cut(s) | 420, 1242 |
| MaeI | CTAG | 5 cut(s) | 96, 408, 608, 1359, 1497 |
| MaeII | ACGT | 3 cut(s) | 404, 932, 1321 |
| MaeIII | GTNAC | 3 cut(s) | 199, 436, 968 |
| MalI | GATC | 7 cut(s) | 77, 143, 150, 157, 1044, 1125, 1148 |
| MbiI | CCGCTC | 1 cut(s) | 493 |
| MboI | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| MboII | GAAGA | 8 cut(s) | 224, 253, 500, 547, 673, 683, 1289, 1478 |
| MfeI | CAATTG | 1 cut(s) | 632 |
| MhlI | GDGCHC | 5 cut(s) | 236, 616, 1101, 1136, 1366 |
| MlsI | TGGCCA | 2 cut(s) | 755, 1160 |
| MluCI | AATT | 6 cut(s) | 267, 514, 632, 1019, 1108, 1182 |
| MluNI | TGGCCA | 2 cut(s) | 755, 1160 |
| MlyI | GAGTC | 1 cut(s) | 62 |
| MmeI | TCCRAC | 1 cut(s) | 20 |
| Mox20I | TGGCCA | 2 cut(s) | 755, 1160 |
| MscI | TGGCCA | 2 cut(s) | 755, 1160 |
| MseI | TTAA | 1 cut(s) | 498 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1220, 1371 |
| Msp20I | TGGCCA | 2 cut(s) | 755, 1160 |
| MspA1I | CMGCKG | 3 cut(s) | 26, 848, 871 |
| MspI | CCGG | 5 cut(s) | 72, 104, 207, 992, 1210 |
| MunI | CAATTG | 1 cut(s) | 632 |
| Mva1269I | GAATGC | 1 cut(s) | 880 |
| MvaI | CCWGG | 7 cut(s) | 91, 419, 827, 1104, 1179, 1302, 1308 |
| MvnI | CGCG | 5 cut(s) | 26, 428, 713, 743, 1481 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 431, 1261, 1545 |
| NciI | CCSGG | 4 cut(s) | 73, 105, 1210, 1211 |
| NdeI | CATATG | 1 cut(s) | 913 |
| NdeII | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| NlaIII | CATG | 6 cut(s) | 646, 671, 760, 1225, 1250, 1411 |
| NlaIV | GGNNCC | 5 cut(s) | 22, 233, 285, 733, 1364 |
| NmuCI | GTSAC | 2 cut(s) | 199, 968 |
| NruI | TCGCGA | 1 cut(s) | 743 |
| PaeR7I | CTCGAG | 1 cut(s) | 879 |
| PasI | CCCWGGG | 1 cut(s) | 1103 |
| PciSI | GCTCTTC | 2 cut(s) | 483, 666 |
| PctI | GAATGC | 1 cut(s) | 880 |
| PfeI | GAWTC | 3 cut(s) | 160, 295, 812 |
| PflFI | GACNNNGTC | 1 cut(s) | 887 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 995 |
| PfoI | TCCNGGA | 2 cut(s) | 71, 89 |
| PleI | GAGTC | 1 cut(s) | 62 |
| PpsI | GAGTC | 1 cut(s) | 62 |
| Psp124BI | GAGCTC | 1 cut(s) | 1101 |
| Psp6I | CCWGG | 7 cut(s) | 89, 417, 825, 1102, 1177, 1300, 1306 |
| PspFI | CCCAGC | 1 cut(s) | 381 |
| PspGI | CCWGG | 7 cut(s) | 89, 417, 825, 1102, 1177, 1300, 1306 |
| PspN4I | GGNNCC | 5 cut(s) | 22, 233, 285, 733, 1364 |
| PspOMI | GGGCCC | 1 cut(s) | 1362 |
| PspPI | GGNCC | 8 cut(s) | 21, 559, 732, 1270, 1304, 1362, 1363, 1388 |
| PsyI | GACNNNGTC | 1 cut(s) | 887 |
| PvuII | CAGCTG | 1 cut(s) | 848 |
| RruI | TCGCGA | 1 cut(s) | 743 |
| RsaI | GTAC | 3 cut(s) | 12, 867, 1523 |
| RsaNI | GTAC | 3 cut(s) | 11, 866, 1522 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1220, 1371 |
| SacI | GAGCTC | 1 cut(s) | 1101 |
| SacII | CCGCGG | 1 cut(s) | 27 |
| SalI | GTCGAC | 2 cut(s) | 65, 564 |
| SapI | GCTCTTC | 2 cut(s) | 483, 666 |
| SaqAI | TTAA | 1 cut(s) | 498 |
| Sau3AI | GATC | 7 cut(s) | 75, 141, 148, 155, 1042, 1123, 1146 |
| Sau96I | GGNCC | 8 cut(s) | 21, 559, 732, 1270, 1304, 1362, 1363, 1388 |
| SchI | GAGTC | 1 cut(s) | 62 |
| SduI | GDGCHC | 5 cut(s) | 236, 616, 1101, 1136, 1366 |
| SfaNI | GCATC | 2 cut(s) | 420, 1242 |
| SfcI | CTRYAG | 1 cut(s) | 924 |
| Sfr274I | CTCGAG | 1 cut(s) | 879 |
| Sfr303I | CCGCGG | 1 cut(s) | 27 |
| SgrBI | CCGCGG | 1 cut(s) | 27 |
| SgrDI | CGTCGACG | 1 cut(s) | 564 |
| SinI | GGWCC | 3 cut(s) | 21, 559, 732 |
| SlaI | CTCGAG | 1 cut(s) | 879 |
| SmaI | CCCGGG | 1 cut(s) | 1211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1220, 1371 |
| SmlI | CTYRAG | 3 cut(s) | 602, 879, 1541 |
| SmoI | CTYRAG | 3 cut(s) | 602, 879, 1541 |
| Sse9I | AATT | 6 cut(s) | 267, 514, 632, 1019, 1108, 1182 |
| SspI | AATATT | 1 cut(s) | 1204 |
| SspMI | CTAG | 5 cut(s) | 96, 408, 608, 1359, 1497 |
| SstI | GAGCTC | 1 cut(s) | 1101 |
| StyI | CCWWGG | 3 cut(s) | 783, 1161, 1358 |
| TaaI | ACNGT | 4 cut(s) | 38, 563, 1034, 1282 |
| TaiI | ACGT | 3 cut(s) | 407, 935, 1324 |
| TaqI | TCGA | 5 cut(s) | 66, 158, 323, 565, 880 |
| TaqII | GACCGA | 1 cut(s) | 580 |
| TasI | AATT | 6 cut(s) | 267, 514, 632, 1019, 1108, 1182 |
| TatI | WGTACW | 1 cut(s) | 10 |
| TauI | GCSGC | 6 cut(s) | 380, 428, 496, 716, 1484, 1518 |
| TfiI | GAWTC | 3 cut(s) | 160, 295, 812 |
| Tru1I | TTAA | 1 cut(s) | 498 |
| Tru9I | TTAA | 1 cut(s) | 498 |
| TscAI | CASTG | 2 cut(s) | 151, 1156 |
| TseFI | GTSAC | 2 cut(s) | 199, 968 |
| TseI | GCWGC | 4 cut(s) | 521, 760, 1286, 1577 |
| Tsp45I | GTSAC | 2 cut(s) | 199, 968 |
| TspDTI | ATGAA | 4 cut(s) | 47, 659, 684, 1398 |
| TspMI | CCCGGG | 1 cut(s) | 1209 |
| TspRI | CASTG | 2 cut(s) | 151, 1156 |
| Tth111I | GACNNNGTC | 1 cut(s) | 887 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 995 |
| VpaK11BI | GGWCC | 3 cut(s) | 21, 559, 732 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 554, 1305 |
| XapI | RAATTY | 2 cut(s) | 514, 1019 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 823 |
| XhoI | CTCGAG | 1 cut(s) | 879 |
| XmaI | CCCGGG | 1 cut(s) | 1209 |
| XmaJI | CCTAGG | 1 cut(s) | 1358 |
| XmiI | GTMKAC | 3 cut(s) | 66, 565, 935 |
| XspI | CTAG | 5 cut(s) | 96, 408, 608, 1359, 1497 |