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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 329 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 607 |
| AccII | CGCG | 2 cut(s) | 143, 305 |
| AciI | CCGC | 2 cut(s) | 7, 404 |
| AclWI | GGATC | 1 cut(s) | 362 |
| AcoI | YGGCCR | 1 cut(s) | 598 |
| AfaI | GTAC | 3 cut(s) | 358, 389, 495 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 607, 626 |
| AgsI | TTSAA | 2 cut(s) | 296, 518 |
| AjnI | CCWGG | 2 cut(s) | 573, 625 |
| AluBI | AGCT | 7 cut(s) | 73, 122, 157, 299, 396, 479, 673 |
| AluI | AGCT | 7 cut(s) | 73, 122, 157, 299, 396, 479, 673 |
| Alw26I | GTCTC | 1 cut(s) | 92 |
| AlwI | GGATC | 1 cut(s) | 362 |
| AoxI | GGCC | 4 cut(s) | 240, 598, 629, 687 |
| ApaI | GGGCCC | 1 cut(s) | 244 |
| ApeKI | GCWGC | 2 cut(s) | 4, 119 |
| AspA2I | CCTAGG | 1 cut(s) | 683 |
| AspLEI | GCGC | 2 cut(s) | 180, 279 |
| AspS9I | GGNCC | 5 cut(s) | 240, 241, 537, 629, 687 |
| AsuC2I | CCSGG | 1 cut(s) | 464 |
| AsuHPI | GGTGA | 1 cut(s) | 250 |
| AvaII | GGWCC | 1 cut(s) | 537 |
| AvrII | CCTAGG | 1 cut(s) | 683 |
| BaeGI | GKGCMC | 1 cut(s) | 244 |
| BanII | GRGCYC | 1 cut(s) | 244 |
| BbsI | GAAGAC | 1 cut(s) | 335 |
| BbvCI | CCTCAGC | 1 cut(s) | 195 |
| BbvI | GCAGC | 1 cut(s) | 131 |
| BccI | CCATC | 1 cut(s) | 347 |
| BciT130I | CCWGG | 2 cut(s) | 575, 627 |
| BciVI | GTATCC | 1 cut(s) | 193 |
| BclI | TGATCA | 1 cut(s) | 448 |
| BcnI | CCSGG | 1 cut(s) | 464 |
| BcoDI | GTCTC | 1 cut(s) | 92 |
| BfaI | CTAG | 4 cut(s) | 170, 249, 570, 684 |
| BfoI | RGCGCY | 1 cut(s) | 280 |
| BfuAI | ACCTGC | 1 cut(s) | 329 |
| BfuI | GTATCC | 1 cut(s) | 193 |
| BisI | GCNGC | 2 cut(s) | 5, 120 |
| BlnI | CCTAGG | 1 cut(s) | 683 |
| BlsI | GCNGC | 2 cut(s) | 6, 121 |
| Bme1390I | CCNGG | 3 cut(s) | 464, 575, 627 |
| Bme18I | GGWCC | 1 cut(s) | 537 |
| BmgT120I | GGNCC | 5 cut(s) | 240, 241, 537, 629, 687 |
| BmiI | GGNNCC | 1 cut(s) | 242 |
| BmrFI | CCNGG | 3 cut(s) | 464, 575, 627 |
| BmsI | GCATC | 2 cut(s) | 603, 635 |
| BpiI | GAAGAC | 1 cut(s) | 335 |
| Bpu10I | CCTNAGC | 1 cut(s) | 195 |
| BpuMI | CCSGG | 1 cut(s) | 464 |
| BsaAI | YACGTR | 1 cut(s) | 164 |
| BsaJI | CCNNGG | 4 cut(s) | 533, 547, 626, 683 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 607, 626 |
| Bse3DI | GCAATG | 2 cut(s) | 180, 584 |
| BseBI | CCWGG | 2 cut(s) | 575, 627 |
| BseDI | CCNNGG | 4 cut(s) | 533, 547, 626, 683 |
| BseGI | GGATG | 2 cut(s) | 358, 496 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 607, 626 |
| BseMI | GCAATG | 2 cut(s) | 180, 584 |
| BseMII | CTCAG | 2 cut(s) | 186, 398 |
| BseRI | GAGGAG | 1 cut(s) | 609 |
| BseSI | GKGCMC | 1 cut(s) | 244 |
| BseXI | GCAGC | 1 cut(s) | 131 |
| BseYI | CCCAGC | 1 cut(s) | 186 |
| Bsh1236I | CGCG | 2 cut(s) | 143, 305 |
| BshFI | GGCC | 4 cut(s) | 242, 600, 631, 689 |
| BsiSI | CCGG | 1 cut(s) | 464 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 607, 626 |
| BsmAI | GTCTC | 1 cut(s) | 92 |
| BsmI | GAATGC | 1 cut(s) | 235 |
| BsnI | GGCC | 4 cut(s) | 242, 600, 631, 689 |
| Bsp120I | GGGCCC | 1 cut(s) | 240 |
| Bsp1286I | GDGCHC | 1 cut(s) | 244 |
| Bsp143I | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| Bsp68I | TCGCGA | 2 cut(s) | 143, 305 |
| BspACI | CCGC | 2 cut(s) | 7, 404 |
| BspANI | GGCC | 4 cut(s) | 242, 600, 631, 689 |
| BspCNI | CTCAG | 2 cut(s) | 187, 397 |
| BspFNI | CGCG | 2 cut(s) | 143, 305 |
| BspHI | TCATGA | 1 cut(s) | 418 |
| BspLI | GGNNCC | 1 cut(s) | 242 |
| BspMI | ACCTGC | 1 cut(s) | 329 |
| BspPI | GGATC | 1 cut(s) | 362 |
| BsrDI | GCAATG | 2 cut(s) | 180, 584 |
| BssECI | CCNNGG | 4 cut(s) | 533, 547, 626, 683 |
| BssMI | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| BssT1I | CCWWGG | 1 cut(s) | 683 |
| Bst2UI | CCWGG | 2 cut(s) | 575, 627 |
| Bst4CI | ACNGT | 3 cut(s) | 325, 508, 595 |
| Bst6I | CTCTTC | 2 cut(s) | 519, 645 |
| BstBAI | YACGTR | 1 cut(s) | 164 |
| BstC8I | GCNNGC | 3 cut(s) | 192, 307, 675 |
| BstDEI | CTNAG | 3 cut(s) | 195, 204, 384 |
| BstF5I | GGATG | 2 cut(s) | 358, 496 |
| BstFNI | CGCG | 2 cut(s) | 143, 305 |
| BstH2I | RGCGCY | 1 cut(s) | 280 |
| BstHHI | GCGC | 2 cut(s) | 180, 279 |
| BstKTI | GATC | 5 cut(s) | 81, 175, 224, 370, 451 |
| BstMAI | GTCTC | 1 cut(s) | 92 |
| BstMBI | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 191, 239, 274, 311 |
| BstNI | CCWGG | 2 cut(s) | 575, 627 |
| BstSCI | CCNGG | 3 cut(s) | 462, 573, 625 |
| BstSLI | GKGCMC | 1 cut(s) | 244 |
| BstUI | CGCG | 2 cut(s) | 143, 305 |
| BstV1I | GCAGC | 1 cut(s) | 131 |
| BstV2I | GAAGAC | 1 cut(s) | 335 |
| BsuI | GTATCC | 1 cut(s) | 193 |
| BsuRI | GGCC | 4 cut(s) | 242, 600, 631, 689 |
| BtgZI | GCGATG | 1 cut(s) | 626 |
| BtsCI | GGATG | 2 cut(s) | 358, 496 |
| BtuMI | TCGCGA | 2 cut(s) | 143, 305 |
| BveI | ACCTGC | 1 cut(s) | 329 |
| Cac8I | GCNNGC | 3 cut(s) | 192, 307, 675 |
| CciI | TCATGA | 1 cut(s) | 418 |
| CfoI | GCGC | 2 cut(s) | 180, 279 |
| Cfr13I | GGNCC | 5 cut(s) | 240, 241, 537, 629, 687 |
| Csp6I | GTAC | 3 cut(s) | 357, 388, 494 |
| CviAII | CATG | 3 cut(s) | 85, 245, 419 |
| CviQI | GTAC | 3 cut(s) | 357, 388, 494 |
| DdeI | CTNAG | 3 cut(s) | 195, 204, 384 |
| DpnI | GATC | 5 cut(s) | 80, 174, 223, 369, 450 |
| DpnII | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| EaeI | YGGCCR | 1 cut(s) | 598 |
| Eam1104I | CTCTTC | 2 cut(s) | 519, 645 |
| EarI | CTCTTC | 2 cut(s) | 519, 645 |
| EciI | GGCGGA | 1 cut(s) | 419 |
| Eco130I | CCWWGG | 1 cut(s) | 683 |
| Eco24I | GRGCYC | 1 cut(s) | 244 |
| Eco47I | GGWCC | 1 cut(s) | 537 |
| EcoO109I | RGGNCCY | 2 cut(s) | 240, 687 |
| EcoRII | CCWGG | 2 cut(s) | 573, 625 |
| EcoT14I | CCWWGG | 1 cut(s) | 683 |
| EcoT38I | GRGCYC | 1 cut(s) | 244 |
| ErhI | CCWWGG | 1 cut(s) | 683 |
| FaeI | CATG | 3 cut(s) | 88, 248, 422 |
| FaiI | YATR | 8 cut(s) | 86, 161, 246, 270, 399, 420, 441, 639 |
| FatI | CATG | 3 cut(s) | 84, 244, 418 |
| FbaI | TGATCA | 1 cut(s) | 448 |
| Fnu4HI | GCNGC | 2 cut(s) | 5, 120 |
| FokI | GGATG | 2 cut(s) | 365, 503 |
| FriOI | GRGCYC | 1 cut(s) | 244 |
| Fsp4HI | GCNGC | 2 cut(s) | 5, 120 |
| FspBI | CTAG | 4 cut(s) | 170, 249, 570, 684 |
| GlaI | GCGC | 2 cut(s) | 179, 278 |
| GluI | GCNGC | 2 cut(s) | 5, 120 |
| GsaI | CCCAGC | 1 cut(s) | 190 |
| HaeII | RGCGCY | 1 cut(s) | 280 |
| HaeIII | GGCC | 4 cut(s) | 242, 600, 631, 689 |
| HapII | CCGG | 1 cut(s) | 464 |
| HhaI | GCGC | 2 cut(s) | 180, 279 |
| Hin1II | CATG | 3 cut(s) | 88, 248, 422 |
| Hin6I | GCGC | 2 cut(s) | 178, 277 |
| HinP1I | GCGC | 2 cut(s) | 178, 277 |
| HpaII | CCGG | 1 cut(s) | 464 |
| HphI | GGTGA | 1 cut(s) | 250 |
| Hpy166II | GTNNAC | 2 cut(s) | 167, 381 |
| Hpy188I | TCNGA | 5 cut(s) | 226, 331, 453, 537, 551 |
| Hpy188III | TCNNGA | 6 cut(s) | 47, 76, 142, 304, 419, 469 |
| Hpy8I | GTNNAC | 2 cut(s) | 167, 381 |
| HpyAV | CCTTC | 1 cut(s) | 548 |
| HpyCH4III | ACNGT | 3 cut(s) | 325, 508, 595 |
| HpyCH4IV | ACGT | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 3 cut(s) | 218, 233, 589 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 191, 239, 274, 311 |
| HpyF3I | CTNAG | 3 cut(s) | 195, 204, 384 |
| HpySE526I | ACGT | 1 cut(s) | 163 |
| Hsp92II | CATG | 3 cut(s) | 88, 248, 422 |
| HspAI | GCGC | 2 cut(s) | 178, 277 |
| Ksp22I | TGATCA | 1 cut(s) | 448 |
| Kzo9I | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| LmnI | GCTCC | 1 cut(s) | 53 |
| Lsp1109I | GCAGC | 1 cut(s) | 131 |
| LweI | GCATC | 2 cut(s) | 603, 635 |
| MaeI | CTAG | 4 cut(s) | 170, 249, 570, 684 |
| MaeII | ACGT | 1 cut(s) | 163 |
| MaeIII | GTNAC | 1 cut(s) | 508 |
| MalI | GATC | 5 cut(s) | 80, 174, 223, 369, 450 |
| MboI | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| MboII | GAAGA | 4 cut(s) | 28, 335, 506, 662 |
| MhlI | GDGCHC | 1 cut(s) | 244 |
| MluCI | AATT | 2 cut(s) | 213, 433 |
| MseI | TTAA | 1 cut(s) | 657 |
| MspA1I | CMGCKG | 1 cut(s) | 122 |
| MspI | CCGG | 1 cut(s) | 464 |
| MspR9I | CCNGG | 3 cut(s) | 464, 575, 627 |
| Mva1269I | GAATGC | 1 cut(s) | 235 |
| MvaI | CCWGG | 2 cut(s) | 575, 627 |
| MvnI | CGCG | 2 cut(s) | 143, 305 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 191, 239, 274, 311 |
| NciI | CCSGG | 1 cut(s) | 464 |
| NdeII | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| NlaIII | CATG | 3 cut(s) | 88, 248, 422 |
| NlaIV | GGNNCC | 1 cut(s) | 242 |
| NruI | TCGCGA | 2 cut(s) | 143, 305 |
| PagI | TCATGA | 1 cut(s) | 418 |
| PctI | GAATGC | 1 cut(s) | 235 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 607 |
| PkrI | GCNGC | 2 cut(s) | 6, 121 |
| Ppu21I | YACGTR | 1 cut(s) | 164 |
| Psp6I | CCWGG | 2 cut(s) | 573, 625 |
| PspFI | CCCAGC | 1 cut(s) | 186 |
| PspGI | CCWGG | 2 cut(s) | 573, 625 |
| PspN4I | GGNNCC | 1 cut(s) | 242 |
| PspOMI | GGGCCC | 1 cut(s) | 240 |
| PspPI | GGNCC | 5 cut(s) | 240, 241, 537, 629, 687 |
| PvuII | CAGCTG | 1 cut(s) | 122 |
| RruI | TCGCGA | 2 cut(s) | 143, 305 |
| RsaI | GTAC | 3 cut(s) | 358, 389, 495 |
| RsaNI | GTAC | 3 cut(s) | 357, 388, 494 |
| SaqAI | TTAA | 1 cut(s) | 657 |
| SatI | GCNGC | 2 cut(s) | 5, 120 |
| Sau3AI | GATC | 5 cut(s) | 78, 172, 221, 367, 448 |
| Sau96I | GGNCC | 5 cut(s) | 240, 241, 537, 629, 687 |
| ScrFI | CCNGG | 3 cut(s) | 464, 575, 627 |
| SduI | GDGCHC | 1 cut(s) | 244 |
| SfaNI | GCATC | 2 cut(s) | 603, 635 |
| SinI | GGWCC | 1 cut(s) | 537 |
| Sse9I | AATT | 2 cut(s) | 213, 433 |
| SsiI | CCGC | 2 cut(s) | 7, 404 |
| SspMI | CTAG | 4 cut(s) | 170, 249, 570, 684 |
| StyD4I | CCNGG | 3 cut(s) | 462, 573, 625 |
| StyI | CCWWGG | 1 cut(s) | 683 |
| TaaI | ACNGT | 3 cut(s) | 325, 508, 595 |
| TaiI | ACGT | 1 cut(s) | 166 |
| TasI | AATT | 2 cut(s) | 213, 433 |
| Tru1I | TTAA | 1 cut(s) | 657 |
| Tru9I | TTAA | 1 cut(s) | 657 |
| TseI | GCWGC | 2 cut(s) | 4, 119 |
| TspDTI | ATGAA | 1 cut(s) | 435 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 607 |
| VpaK11BI | GGWCC | 1 cut(s) | 537 |
| XmaJI | CCTAGG | 1 cut(s) | 683 |
| XspI | CTAG | 4 cut(s) | 170, 249, 570, 684 |