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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 225 |
| AccI | GTMKAC | 3 cut(s) | 9, 364, 584 |
| AccII | CGCG | 1 cut(s) | 437 |
| AciI | CCGC | 2 cut(s) | 318, 507 |
| AclWI | GGATC | 3 cut(s) | 31, 276, 289 |
| AcuI | CTGAAG | 2 cut(s) | 315, 486 |
| AcyI | GRCGYC | 1 cut(s) | 222 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 579 |
| AjnI | CCWGG | 2 cut(s) | 71, 457 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 312, 344 |
| AluBI | AGCT | 2 cut(s) | 383, 592 |
| AluI | AGCT | 2 cut(s) | 383, 592 |
| Alw26I | GTCTC | 1 cut(s) | 140 |
| AlwI | GGATC | 3 cut(s) | 31, 276, 289 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 464 |
| ApeKI | GCWGC | 1 cut(s) | 473 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 207, 239 |
| AspS9I | GGNCC | 2 cut(s) | 509, 603 |
| AsuHPI | GGTGA | 3 cut(s) | 193, 217, 512 |
| AvaII | GGWCC | 2 cut(s) | 509, 603 |
| BamHI | GGATCC | 1 cut(s) | 281 |
| BbvI | GCAGC | 1 cut(s) | 485 |
| BccI | CCATC | 1 cut(s) | 158 |
| BciT130I | CCWGG | 2 cut(s) | 73, 459 |
| BclI | TGATCA | 2 cut(s) | 103, 376 |
| BcoDI | GTCTC | 1 cut(s) | 140 |
| BfaI | CTAG | 1 cut(s) | 63 |
| BfmI | CTRYAG | 1 cut(s) | 6 |
| BisI | GCNGC | 1 cut(s) | 474 |
| BlsI | GCNGC | 1 cut(s) | 475 |
| Bme1390I | CCNGG | 2 cut(s) | 73, 459 |
| Bme18I | GGWCC | 2 cut(s) | 509, 603 |
| BmgT120I | GGNCC | 2 cut(s) | 509, 603 |
| BmiI | GGNNCC | 3 cut(s) | 283, 494, 604 |
| BmrFI | CCNGG | 2 cut(s) | 73, 459 |
| BmsI | GCATC | 2 cut(s) | 246, 460 |
| BpuEI | CTTGAG | 1 cut(s) | 354 |
| BsaHI | GRCGYC | 1 cut(s) | 222 |
| BsaI | GGTCTC | 1 cut(s) | 140 |
| BsaJI | CCNNGG | 2 cut(s) | 458, 496 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 579 |
| Bse118I | RCCGGY | 1 cut(s) | 427 |
| Bse1I | ACTGG | 1 cut(s) | 404 |
| BseBI | CCWGG | 2 cut(s) | 73, 459 |
| BseDI | CCNNGG | 2 cut(s) | 458, 496 |
| BseGI | GGATG | 1 cut(s) | 554 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 579 |
| BseMII | CTCAG | 1 cut(s) | 135 |
| BseNI | ACTGG | 1 cut(s) | 404 |
| BseRI | GAGGAG | 1 cut(s) | 375 |
| BseXI | GCAGC | 1 cut(s) | 485 |
| Bsh1236I | CGCG | 1 cut(s) | 437 |
| BsiSI | CCGG | 1 cut(s) | 428 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 579 |
| BsmAI | GTCTC | 1 cut(s) | 140 |
| BsmI | GAATGC | 2 cut(s) | 64, 509 |
| Bso31I | GGTCTC | 1 cut(s) | 140 |
| Bsp143I | GATC | 4 cut(s) | 36, 103, 281, 376 |
| Bsp68I | TCGCGA | 1 cut(s) | 437 |
| BspACI | CCGC | 2 cut(s) | 318, 507 |
| BspCNI | CTCAG | 1 cut(s) | 136 |
| BspFNI | CGCG | 1 cut(s) | 437 |
| BspLI | GGNNCC | 3 cut(s) | 283, 494, 604 |
| BspPI | GGATC | 3 cut(s) | 31, 276, 289 |
| BspTNI | GGTCTC | 1 cut(s) | 140 |
| BsrFI | RCCGGY | 1 cut(s) | 427 |
| BsrI | ACTGG | 1 cut(s) | 404 |
| BssAI | RCCGGY | 1 cut(s) | 427 |
| BssECI | CCNNGG | 2 cut(s) | 458, 496 |
| BssMI | GATC | 4 cut(s) | 36, 103, 281, 376 |
| BssNAI | GTATAC | 1 cut(s) | 585 |
| BssNI | GRCGYC | 1 cut(s) | 222 |
| BssT1I | CCWWGG | 1 cut(s) | 496 |
| Bst1107I | GTATAC | 1 cut(s) | 585 |
| Bst2UI | CCWGG | 2 cut(s) | 73, 459 |
| Bst4CI | ACNGT | 1 cut(s) | 175 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 45 |
| BstACI | GRCGYC | 1 cut(s) | 222 |
| BstC8I | GCNNGC | 1 cut(s) | 429 |
| BstDEI | CTNAG | 3 cut(s) | 144, 389, 539 |
| BstF5I | GGATG | 1 cut(s) | 554 |
| BstFNI | CGCG | 1 cut(s) | 437 |
| BstKTI | GATC | 4 cut(s) | 39, 106, 284, 379 |
| BstMAI | GTCTC | 1 cut(s) | 140 |
| BstMBI | GATC | 4 cut(s) | 36, 103, 281, 376 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 68, 256, 428 |
| BstNI | CCWGG | 2 cut(s) | 73, 459 |
| BstNSI | RCATGY | 1 cut(s) | 16 |
| BstSCI | CCNGG | 2 cut(s) | 71, 457 |
| BstSFI | CTRYAG | 1 cut(s) | 6 |
| BstUI | CGCG | 1 cut(s) | 437 |
| BstV1I | GCAGC | 1 cut(s) | 485 |
| BstX2I | RGATCY | 1 cut(s) | 281 |
| BstYI | RGATCY | 1 cut(s) | 281 |
| BstZ17I | GTATAC | 1 cut(s) | 585 |
| BtsCI | GGATG | 1 cut(s) | 554 |
| BtuMI | TCGCGA | 1 cut(s) | 437 |
| Cac8I | GCNNGC | 1 cut(s) | 429 |
| CaiI | CAGNNNCTG | 1 cut(s) | 464 |
| Cfr10I | RCCGGY | 1 cut(s) | 427 |
| Cfr13I | GGNCC | 2 cut(s) | 509, 603 |
| CviAII | CATG | 3 cut(s) | 13, 47, 579 |
| CviJI | RGCY | 9 cut(s) | 76, 89, 118, 250, 383, 403, 427, 572, 592 |
| CviKI_1 | RGCY | 9 cut(s) | 76, 89, 118, 250, 383, 403, 427, 572, 592 |
| DdeI | CTNAG | 3 cut(s) | 144, 389, 539 |
| DpnI | GATC | 4 cut(s) | 38, 105, 283, 378 |
| DpnII | GATC | 4 cut(s) | 36, 103, 281, 376 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 45 |
| EarI | CTCTTC | 2 cut(s) | 24, 45 |
| EciI | GGCGGA | 1 cut(s) | 333 |
| Eco130I | CCWWGG | 1 cut(s) | 496 |
| Eco31I | GGTCTC | 1 cut(s) | 140 |
| Eco47I | GGWCC | 2 cut(s) | 509, 603 |
| Eco57I | CTGAAG | 2 cut(s) | 315, 486 |
| EcoRII | CCWGG | 2 cut(s) | 71, 457 |
| EcoT14I | CCWWGG | 1 cut(s) | 496 |
| ErhI | CCWWGG | 1 cut(s) | 496 |
| FaeI | CATG | 3 cut(s) | 16, 50, 582 |
| FatI | CATG | 3 cut(s) | 12, 46, 578 |
| FbaI | TGATCA | 2 cut(s) | 103, 376 |
| FblI | GTMKAC | 3 cut(s) | 9, 364, 584 |
| Fnu4HI | GCNGC | 1 cut(s) | 474 |
| FokI | GGATG | 1 cut(s) | 541 |
| Fsp4HI | GCNGC | 1 cut(s) | 474 |
| FspBI | CTAG | 1 cut(s) | 63 |
| GluI | GCNGC | 1 cut(s) | 474 |
| HapII | CCGG | 1 cut(s) | 428 |
| Hin1I | GRCGYC | 1 cut(s) | 222 |
| Hin1II | CATG | 3 cut(s) | 16, 50, 582 |
| HincII | GTYRAC | 1 cut(s) | 451 |
| HindII | GTYRAC | 1 cut(s) | 451 |
| HinfI | GANTC | 2 cut(s) | 289, 551 |
| HpaI | GTTAAC | 1 cut(s) | 451 |
| HpaII | CCGG | 1 cut(s) | 428 |
| HphI | GGTGA | 3 cut(s) | 193, 217, 512 |
| Hpy166II | GTNNAC | 6 cut(s) | 10, 199, 245, 365, 451, 585 |
| Hpy188I | TCNGA | 3 cut(s) | 41, 82, 466 |
| Hpy188III | TCNNGA | 3 cut(s) | 293, 436, 611 |
| Hpy8I | GTNNAC | 6 cut(s) | 10, 199, 245, 365, 451, 585 |
| Hpy99I | CGWCG | 1 cut(s) | 256 |
| HpyAV | CCTTC | 2 cut(s) | 36, 172 |
| HpyCH4III | ACNGT | 1 cut(s) | 175 |
| HpyCH4IV | ACGT | 1 cut(s) | 222 |
| HpyCH4V | TGCA | 3 cut(s) | 16, 213, 473 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 68, 256, 428 |
| HpyF3I | CTNAG | 3 cut(s) | 144, 389, 539 |
| HpySE526I | ACGT | 1 cut(s) | 222 |
| Hsp92I | GRCGYC | 1 cut(s) | 222 |
| Hsp92II | CATG | 3 cut(s) | 16, 50, 582 |
| KroI | GCCGGC | 1 cut(s) | 427 |
| KroNI | GCCGGC | 1 cut(s) | 429 |
| Ksp22I | TGATCA | 2 cut(s) | 103, 376 |
| KspAI | GTTAAC | 1 cut(s) | 451 |
| Kzo9I | GATC | 4 cut(s) | 36, 103, 281, 376 |
| LmnI | GCTCC | 1 cut(s) | 388 |
| LpnPI | CCDG | 9 cut(s) | 58, 85, 100, 279, 306, 417, 441, 444, 471 |
| Lsp1109I | GCAGC | 1 cut(s) | 485 |
| LweI | GCATC | 2 cut(s) | 246, 460 |
| MaeI | CTAG | 1 cut(s) | 63 |
| MaeII | ACGT | 1 cut(s) | 222 |
| MaeIII | GTNAC | 2 cut(s) | 223, 337 |
| MalI | GATC | 4 cut(s) | 38, 105, 283, 378 |
| MboI | GATC | 4 cut(s) | 36, 103, 281, 376 |
| MboII | GAAGA | 4 cut(s) | 41, 62, 245, 479 |
| MflI | RGATCY | 1 cut(s) | 281 |
| MnlI | CCTC | 8 cut(s) | 46, 127, 160, 349, 378, 396, 432, 483 |
| MroNI | GCCGGC | 1 cut(s) | 427 |
| MseI | TTAA | 2 cut(s) | 162, 450 |
| MspI | CCGG | 1 cut(s) | 428 |
| MspR9I | CCNGG | 2 cut(s) | 73, 459 |
| Mva1269I | GAATGC | 2 cut(s) | 64, 509 |
| MvaI | CCWGG | 2 cut(s) | 73, 459 |
| MvnI | CGCG | 1 cut(s) | 437 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 68, 256, 428 |
| NaeI | GCCGGC | 1 cut(s) | 429 |
| NdeII | GATC | 4 cut(s) | 36, 103, 281, 376 |
| NgoMIV | GCCGGC | 1 cut(s) | 427 |
| NlaIII | CATG | 3 cut(s) | 16, 50, 582 |
| NlaIV | GGNNCC | 3 cut(s) | 283, 494, 604 |
| NmuCI | GTSAC | 2 cut(s) | 223, 337 |
| NruI | TCGCGA | 1 cut(s) | 437 |
| NspI | RCATGY | 1 cut(s) | 16 |
| PctI | GAATGC | 2 cut(s) | 64, 509 |
| PdiI | GCCGGC | 1 cut(s) | 429 |
| PfeI | GAWTC | 2 cut(s) | 289, 551 |
| PkrI | GCNGC | 1 cut(s) | 475 |
| Psp6I | CCWGG | 2 cut(s) | 71, 457 |
| PspGI | CCWGG | 2 cut(s) | 71, 457 |
| PspN4I | GGNNCC | 3 cut(s) | 283, 494, 604 |
| PspPI | GGNCC | 2 cut(s) | 509, 603 |
| PstNI | CAGNNNCTG | 1 cut(s) | 464 |
| PsuI | RGATCY | 1 cut(s) | 281 |
| RruI | TCGCGA | 1 cut(s) | 437 |
| SaqAI | TTAA | 2 cut(s) | 162, 450 |
| SatI | GCNGC | 1 cut(s) | 474 |
| Sau3AI | GATC | 4 cut(s) | 36, 103, 281, 376 |
| Sau96I | GGNCC | 2 cut(s) | 509, 603 |
| ScrFI | CCNGG | 2 cut(s) | 73, 459 |
| SetI | ASST | 8 cut(s) | 183, 225, 230, 370, 385, 494, 502, 594 |
| SfaNI | GCATC | 2 cut(s) | 246, 460 |
| SfcI | CTRYAG | 1 cut(s) | 6 |
| SinI | GGWCC | 2 cut(s) | 509, 603 |
| SmlI | CTYRAG | 1 cut(s) | 369 |
| SmoI | CTYRAG | 1 cut(s) | 369 |
| SsiI | CCGC | 2 cut(s) | 318, 507 |
| SspMI | CTAG | 1 cut(s) | 63 |
| StyD4I | CCNGG | 2 cut(s) | 71, 457 |
| StyI | CCWWGG | 1 cut(s) | 496 |
| TaaI | ACNGT | 1 cut(s) | 175 |
| TaiI | ACGT | 1 cut(s) | 225 |
| TaqI | TCGA | 2 cut(s) | 488, 549 |
| TfiI | GAWTC | 2 cut(s) | 289, 551 |
| Tru1I | TTAA | 2 cut(s) | 162, 450 |
| Tru9I | TTAA | 2 cut(s) | 162, 450 |
| TseFI | GTSAC | 2 cut(s) | 223, 337 |
| TseI | GCWGC | 1 cut(s) | 473 |
| Tsp45I | GTSAC | 2 cut(s) | 223, 337 |
| TspDTI | ATGAA | 4 cut(s) | 38, 63, 246, 543 |
| TspGWI | ACGGA | 1 cut(s) | 294 |
| VpaK11BI | GGWCC | 2 cut(s) | 509, 603 |
| XceI | RCATGY | 1 cut(s) | 16 |
| XmiI | GTMKAC | 3 cut(s) | 9, 364, 584 |
| XspI | CTAG | 1 cut(s) | 63 |
| ZraI | GACGTC | 1 cut(s) | 223 |