Translocon-associated protein subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 604 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 368 |
| AccII | CGCG | 3 cut(s) | 532, 713, 998 |
| AciI | CCGC | 4 cut(s) | 38, 663, 711, 839 |
| AclWI | GGATC | 3 cut(s) | 1011, 1024, 1091 |
| AcoI | YGGCCR | 2 cut(s) | 425, 1038 |
| AcsI | RAATTY | 1 cut(s) | 799 |
| AcuI | CTGAAG | 2 cut(s) | 663, 972 |
| AdeI | CACNNNGTG | 1 cut(s) | 368 |
| AfaI | GTAC | 1 cut(s) | 1148 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 223, 368, 423, 841, 1139 |
| AgsI | TTSAA | 6 cut(s) | 52, 101, 256, 331, 434, 794 |
| AjnI | CCWGG | 4 cut(s) | 422, 702, 1032, 1120 |
| AleI | CACNNNNGTG | 1 cut(s) | 531 |
| AluBI | AGCT | 6 cut(s) | 8, 143, 180, 781, 892, 961 |
| AluI | AGCT | 6 cut(s) | 8, 143, 180, 781, 892, 961 |
| Alw21I | GWGCWC | 1 cut(s) | 170 |
| Alw26I | GTCTC | 1 cut(s) | 1134 |
| AlwI | GGATC | 3 cut(s) | 1011, 1024, 1091 |
| AoxI | GGCC | 4 cut(s) | 108, 353, 425, 1038 |
| ApeKI | GCWGC | 2 cut(s) | 778, 806 |
| ApoI | RAATTY | 1 cut(s) | 799 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 898, 930 |
| AspLEI | GCGC | 3 cut(s) | 464, 719, 1106 |
| AspS9I | GGNCC | 1 cut(s) | 844 |
| AsuHPI | GGTGA | 2 cut(s) | 187, 691 |
| AvaII | GGWCC | 1 cut(s) | 844 |
| BaeGI | GKGCMC | 1 cut(s) | 901 |
| BalI | TGGCCA | 2 cut(s) | 427, 1040 |
| BamHI | GGATCC | 1 cut(s) | 1016 |
| BauI | CACGAG | 1 cut(s) | 199 |
| Bbv12I | GWGCWC | 1 cut(s) | 170 |
| BbvI | GCAGC | 2 cut(s) | 790, 793 |
| BccI | CCATC | 3 cut(s) | 601, 817, 917 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 212, 246, 964, 998 |
| BciT130I | CCWGG | 4 cut(s) | 424, 704, 1034, 1122 |
| BciVI | GTATCC | 1 cut(s) | 679 |
| BclI | TGATCA | 1 cut(s) | 1060 |
| BcoDI | GTCTC | 1 cut(s) | 1134 |
| BfaI | CTAG | 4 cut(s) | 693, 893, 1101, 1112 |
| BfoI | RGCGCY | 1 cut(s) | 1107 |
| BfuI | GTATCC | 1 cut(s) | 679 |
| BglII | AGATCT | 1 cut(s) | 18 |
| BisI | GCNGC | 5 cut(s) | 39, 663, 711, 779, 807 |
| BlpI | GCTNAGC | 1 cut(s) | 181 |
| BlsI | GCNGC | 5 cut(s) | 40, 664, 712, 780, 808 |
| Bme1390I | CCNGG | 4 cut(s) | 424, 704, 1034, 1122 |
| Bme18I | GGWCC | 1 cut(s) | 844 |
| BmgT120I | GGNCC | 1 cut(s) | 844 |
| BmiI | GGNNCC | 1 cut(s) | 1018 |
| BmrFI | CCNGG | 4 cut(s) | 424, 704, 1034, 1122 |
| BmrI | ACTGGG | 1 cut(s) | 1100 |
| BmsI | GCATC | 1 cut(s) | 705 |
| BmuI | ACTGGG | 1 cut(s) | 1100 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 29 |
| BpmI | CTGGAG | 2 cut(s) | 496, 1104 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 181 |
| BpuEI | CTTGAG | 1 cut(s) | 908 |
| BsaJI | CCNNGG | 2 cut(s) | 702, 1032 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 223, 368, 423, 841, 1139 |
| Bse1I | ACTGG | 2 cut(s) | 513, 1095 |
| BseBI | CCWGG | 4 cut(s) | 424, 704, 1034, 1122 |
| BseDI | CCNNGG | 2 cut(s) | 702, 1032 |
| BseGI | GGATG | 2 cut(s) | 631, 928 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 223, 368, 423, 841, 1139 |
| BseMII | CTCAG | 1 cut(s) | 195 |
| BseNI | ACTGG | 2 cut(s) | 513, 1095 |
| BseRI | GAGGAG | 1 cut(s) | 633 |
| BseSI | GKGCMC | 1 cut(s) | 901 |
| BseXI | GCAGC | 2 cut(s) | 790, 793 |
| BseYI | CCCAGC | 1 cut(s) | 680 |
| BsgI | GTGCAG | 3 cut(s) | 509, 555, 962 |
| Bsh1236I | CGCG | 3 cut(s) | 532, 713, 998 |
| BshFI | GGCC | 4 cut(s) | 110, 355, 427, 1040 |
| BsiHKAI | GWGCWC | 1 cut(s) | 170 |
| BslFI | GGGAC | 2 cut(s) | 287, 320 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 223, 368, 423, 841, 1139 |
| BsmAI | GTCTC | 1 cut(s) | 1134 |
| BsmFI | GGGAC | 2 cut(s) | 287, 320 |
| BsnI | GGCC | 4 cut(s) | 110, 355, 427, 1040 |
| Bsp1286I | GDGCHC | 2 cut(s) | 170, 901 |
| Bsp143I | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 181 |
| Bsp68I | TCGCGA | 1 cut(s) | 998 |
| BspACI | CCGC | 4 cut(s) | 38, 663, 711, 839 |
| BspANI | GGCC | 4 cut(s) | 110, 355, 427, 1040 |
| BspCNI | CTCAG | 1 cut(s) | 194 |
| BspFNI | CGCG | 3 cut(s) | 532, 713, 998 |
| BspLI | GGNNCC | 1 cut(s) | 1018 |
| BspPI | GGATC | 3 cut(s) | 1011, 1024, 1091 |
| BspQI | GCTCTTC | 1 cut(s) | 768 |
| BsrI | ACTGG | 2 cut(s) | 513, 1095 |
| BssECI | CCNNGG | 2 cut(s) | 702, 1032 |
| BssMI | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| BssSI | CACGAG | 1 cut(s) | 199 |
| Bst2BI | CACGAG | 1 cut(s) | 199 |
| Bst2UI | CCWGG | 4 cut(s) | 424, 704, 1034, 1122 |
| Bst4CI | ACNGT | 3 cut(s) | 126, 542, 848 |
| Bst6I | CTCTTC | 1 cut(s) | 768 |
| BstC8I | GCNNGC | 4 cut(s) | 345, 534, 715, 973 |
| BstDEI | CTNAG | 1 cut(s) | 181 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 839 |
| BstF5I | GGATG | 2 cut(s) | 631, 928 |
| BstFNI | CGCG | 3 cut(s) | 532, 713, 998 |
| BstH2I | RGCGCY | 1 cut(s) | 1107 |
| BstHHI | GCGC | 3 cut(s) | 464, 719, 1106 |
| BstKTI | GATC | 4 cut(s) | 21, 1019, 1063, 1099 |
| BstMAI | GTCTC | 1 cut(s) | 1134 |
| BstMBI | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 165, 716 |
| BstNI | CCWGG | 4 cut(s) | 424, 704, 1034, 1122 |
| BstSCI | CCNGG | 4 cut(s) | 422, 702, 1032, 1120 |
| BstSLI | GKGCMC | 1 cut(s) | 901 |
| BstUI | CGCG | 3 cut(s) | 532, 713, 998 |
| BstV1I | GCAGC | 2 cut(s) | 790, 793 |
| BstX2I | RGATCY | 3 cut(s) | 18, 1016, 1096 |
| BstYI | RGATCY | 3 cut(s) | 18, 1016, 1096 |
| BsuI | GTATCC | 1 cut(s) | 679 |
| BsuRI | GGCC | 4 cut(s) | 110, 355, 427, 1040 |
| BtgZI | GCGATG | 1 cut(s) | 967 |
| BtsCI | GGATG | 2 cut(s) | 631, 928 |
| BtsIMutI | CAGTG | 1 cut(s) | 1088 |
| BtuMI | TCGCGA | 1 cut(s) | 998 |
| Cac8I | GCNNGC | 4 cut(s) | 345, 534, 715, 973 |
| CfoI | GCGC | 3 cut(s) | 464, 719, 1106 |
| Cfr13I | GGNCC | 1 cut(s) | 844 |
| Csp6I | GTAC | 1 cut(s) | 1147 |
| CviAII | CATG | 8 cut(s) | 57, 160, 218, 381, 815, 928, 1042, 1064 |
| CviQI | GTAC | 1 cut(s) | 1147 |
| DdeI | CTNAG | 1 cut(s) | 181 |
| DpnI | GATC | 4 cut(s) | 20, 1018, 1062, 1098 |
| DpnII | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| DraIII | CACNNNGTG | 1 cut(s) | 368 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 604 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 604 |
| EaeI | YGGCCR | 2 cut(s) | 425, 1038 |
| Eam1104I | CTCTTC | 1 cut(s) | 768 |
| EarI | CTCTTC | 1 cut(s) | 768 |
| Eco147I | AGGCCT | 1 cut(s) | 355 |
| Eco47I | GGWCC | 1 cut(s) | 844 |
| Eco57I | CTGAAG | 2 cut(s) | 663, 972 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 839 |
| EcoRI | GAATTC | 1 cut(s) | 799 |
| EcoRII | CCWGG | 4 cut(s) | 422, 702, 1032, 1120 |
| FaeI | CATG | 8 cut(s) | 60, 163, 221, 384, 818, 931, 1045, 1067 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 765, 797 |
| FaqI | GGGAC | 2 cut(s) | 287, 320 |
| FatI | CATG | 8 cut(s) | 56, 159, 217, 380, 814, 927, 1041, 1063 |
| FbaI | TGATCA | 1 cut(s) | 1060 |
| Fnu4HI | GCNGC | 5 cut(s) | 39, 663, 711, 779, 807 |
| FokI | GGATG | 2 cut(s) | 618, 935 |
| Fsp4HI | GCNGC | 5 cut(s) | 39, 663, 711, 779, 807 |
| FspBI | CTAG | 4 cut(s) | 693, 893, 1101, 1112 |
| GlaI | GCGC | 3 cut(s) | 463, 718, 1105 |
| GluI | GCNGC | 5 cut(s) | 39, 663, 711, 779, 807 |
| GsaI | CCCAGC | 1 cut(s) | 684 |
| GsuI | CTGGAG | 2 cut(s) | 496, 1104 |
| HaeII | RGCGCY | 1 cut(s) | 1107 |
| HaeIII | GGCC | 4 cut(s) | 110, 355, 427, 1040 |
| HhaI | GCGC | 3 cut(s) | 464, 719, 1106 |
| Hin1II | CATG | 8 cut(s) | 60, 163, 221, 384, 818, 931, 1045, 1067 |
| Hin6I | GCGC | 3 cut(s) | 462, 717, 1104 |
| HinP1I | GCGC | 3 cut(s) | 462, 717, 1104 |
| HincII | GTYRAC | 2 cut(s) | 490, 609 |
| HindII | GTYRAC | 2 cut(s) | 490, 609 |
| HinfI | GANTC | 4 cut(s) | 212, 331, 393, 1003 |
| HpaI | GTTAAC | 1 cut(s) | 490 |
| HphI | GGTGA | 2 cut(s) | 187, 691 |
| Hpy166II | GTNNAC | 6 cut(s) | 244, 484, 490, 568, 609, 867 |
| Hpy188I | TCNGA | 1 cut(s) | 991 |
| Hpy188III | TCNNGA | 6 cut(s) | 16, 335, 440, 880, 997, 1027 |
| Hpy8I | GTNNAC | 6 cut(s) | 244, 484, 490, 568, 609, 867 |
| Hpy99I | CGWCG | 4 cut(s) | 287, 852, 1003, 1028 |
| HpyAV | CCTTC | 4 cut(s) | 638, 800, 846, 1042 |
| HpyCH4III | ACNGT | 3 cut(s) | 126, 542, 848 |
| HpyCH4IV | ACGT | 3 cut(s) | 486, 689, 1023 |
| HpyCH4V | TGCA | 7 cut(s) | 137, 347, 526, 536, 809, 943, 1045 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 165, 716 |
| HpyF3I | CTNAG | 1 cut(s) | 181 |
| HpySE526I | ACGT | 3 cut(s) | 486, 689, 1023 |
| Hsp92II | CATG | 8 cut(s) | 60, 163, 221, 384, 818, 931, 1045, 1067 |
| HspAI | GCGC | 3 cut(s) | 462, 717, 1104 |
| Ksp22I | TGATCA | 1 cut(s) | 1060 |
| KspAI | GTTAAC | 1 cut(s) | 490 |
| Kzo9I | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| LguI | GCTCTTC | 1 cut(s) | 768 |
| LmnI | GCTCC | 1 cut(s) | 165 |
| Lsp1109I | GCAGC | 2 cut(s) | 790, 793 |
| LweI | GCATC | 1 cut(s) | 705 |
| MaeI | CTAG | 4 cut(s) | 693, 893, 1101, 1112 |
| MaeII | ACGT | 3 cut(s) | 486, 689, 1023 |
| MaeIII | GTNAC | 3 cut(s) | 515, 721, 1027 |
| MalI | GATC | 4 cut(s) | 20, 1018, 1062, 1098 |
| MboI | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| MboII | GAAGA | 3 cut(s) | 785, 832, 958 |
| MfeI | CAATTG | 1 cut(s) | 917 |
| MflI | RGATCY | 3 cut(s) | 18, 1016, 1096 |
| MhlI | GDGCHC | 2 cut(s) | 170, 901 |
| MlsI | TGGCCA | 2 cut(s) | 427, 1040 |
| MluCI | AATT | 3 cut(s) | 101, 799, 917 |
| MluNI | TGGCCA | 2 cut(s) | 427, 1040 |
| MmeI | TCCRAC | 1 cut(s) | 1044 |
| Mox20I | TGGCCA | 2 cut(s) | 427, 1040 |
| MscI | TGGCCA | 2 cut(s) | 427, 1040 |
| MseI | TTAA | 2 cut(s) | 489, 783 |
| MslI | CAYNNNNRTG | 1 cut(s) | 531 |
| Msp20I | TGGCCA | 2 cut(s) | 427, 1040 |
| MspA1I | CMGCKG | 2 cut(s) | 38, 143 |
| MspR9I | CCNGG | 4 cut(s) | 424, 704, 1034, 1122 |
| MunI | CAATTG | 1 cut(s) | 917 |
| MvaI | CCWGG | 4 cut(s) | 424, 704, 1034, 1122 |
| MvnI | CGCG | 3 cut(s) | 532, 713, 998 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 165, 716 |
| NdeII | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| NlaIII | CATG | 8 cut(s) | 60, 163, 221, 384, 818, 931, 1045, 1067 |
| NlaIV | GGNNCC | 1 cut(s) | 1018 |
| NmuCI | GTSAC | 1 cut(s) | 1027 |
| NruI | TCGCGA | 1 cut(s) | 998 |
| OliI | CACNNNNGTG | 1 cut(s) | 531 |
| PceI | AGGCCT | 1 cut(s) | 355 |
| PciSI | GCTCTTC | 1 cut(s) | 768 |
| PfeI | GAWTC | 4 cut(s) | 212, 331, 393, 1003 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 368 |
| PkrI | GCNGC | 5 cut(s) | 40, 664, 712, 780, 808 |
| Psp6I | CCWGG | 4 cut(s) | 422, 702, 1032, 1120 |
| PspFI | CCCAGC | 1 cut(s) | 680 |
| PspGI | CCWGG | 4 cut(s) | 422, 702, 1032, 1120 |
| PspN4I | GGNNCC | 1 cut(s) | 1018 |
| PspPI | GGNCC | 1 cut(s) | 844 |
| PsuI | RGATCY | 3 cut(s) | 18, 1016, 1096 |
| PvuII | CAGCTG | 1 cut(s) | 143 |
| RruI | TCGCGA | 1 cut(s) | 998 |
| RsaI | GTAC | 1 cut(s) | 1148 |
| RsaNI | GTAC | 1 cut(s) | 1147 |
| RseI | CAYNNNNRTG | 1 cut(s) | 531 |
| SapI | GCTCTTC | 1 cut(s) | 768 |
| SaqAI | TTAA | 2 cut(s) | 489, 783 |
| SatI | GCNGC | 5 cut(s) | 39, 663, 711, 779, 807 |
| Sau3AI | GATC | 4 cut(s) | 18, 1016, 1060, 1096 |
| Sau96I | GGNCC | 1 cut(s) | 844 |
| ScrFI | CCNGG | 4 cut(s) | 424, 704, 1034, 1122 |
| SduI | GDGCHC | 2 cut(s) | 170, 901 |
| SfaNI | GCATC | 1 cut(s) | 705 |
| SinI | GGWCC | 1 cut(s) | 844 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 531 |
| SmlI | CTYRAG | 2 cut(s) | 14, 887 |
| SmoI | CTYRAG | 2 cut(s) | 14, 887 |
| Sse9I | AATT | 3 cut(s) | 101, 799, 917 |
| SseBI | AGGCCT | 1 cut(s) | 355 |
| SsiI | CCGC | 4 cut(s) | 38, 663, 711, 839 |
| SspMI | CTAG | 4 cut(s) | 693, 893, 1101, 1112 |
| StuI | AGGCCT | 1 cut(s) | 355 |
| StyD4I | CCNGG | 4 cut(s) | 422, 702, 1032, 1120 |
| TaaI | ACNGT | 3 cut(s) | 126, 542, 848 |
| TaiI | ACGT | 3 cut(s) | 489, 692, 1026 |
| TaqI | TCGA | 2 cut(s) | 596, 850 |
| TaqII | GACCGA | 1 cut(s) | 865 |
| TasI | AATT | 3 cut(s) | 101, 799, 917 |
| TatI | WGTACW | 1 cut(s) | 1146 |
| TauI | GCSGC | 3 cut(s) | 41, 665, 713 |
| TfiI | GAWTC | 4 cut(s) | 212, 331, 393, 1003 |
| Tru1I | TTAA | 2 cut(s) | 489, 783 |
| Tru9I | TTAA | 2 cut(s) | 489, 783 |
| TscAI | CASTG | 1 cut(s) | 1095 |
| TseFI | GTSAC | 1 cut(s) | 1027 |
| TseI | GCWGC | 2 cut(s) | 778, 806 |
| Tsp45I | GTSAC | 1 cut(s) | 1027 |
| TspDTI | ATGAA | 3 cut(s) | 305, 369, 944 |
| TspRI | CASTG | 1 cut(s) | 1095 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 368 |
| VpaK11BI | GGWCC | 1 cut(s) | 844 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 839 |
| XapI | RAATTY | 1 cut(s) | 799 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 224 |
| XspI | CTAG | 4 cut(s) | 693, 893, 1101, 1112 |