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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 443 |
| Acc65I | GGTACC | 1 cut(s) | 252 |
| AccB1I | GGYRCC | 1 cut(s) | 252 |
| AccBSI | CCGCTC | 2 cut(s) | 168, 556 |
| AccII | CGCG | 3 cut(s) | 109, 363, 533 |
| AclWI | GGATC | 1 cut(s) | 387 |
| AcoI | YGGCCR | 1 cut(s) | 169 |
| AcsI | RAATTY | 1 cut(s) | 79 |
| AfaI | GTAC | 2 cut(s) | 254, 282 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 327 |
| AflIII | ACRYGT | 1 cut(s) | 501 |
| AgsI | TTSAA | 3 cut(s) | 138, 155, 430 |
| AjiI | CACGTC | 1 cut(s) | 504 |
| AjnI | CCWGG | 1 cut(s) | 54 |
| AluBI | AGCT | 3 cut(s) | 122, 315, 460 |
| AluI | AGCT | 3 cut(s) | 122, 315, 460 |
| Alw21I | GWGCWC | 1 cut(s) | 528 |
| AlwI | GGATC | 1 cut(s) | 387 |
| Ama87I | CYCGRG | 1 cut(s) | 557 |
| AoxI | GGCC | 1 cut(s) | 169 |
| ApeKI | GCWGC | 2 cut(s) | 337, 374 |
| ApoI | RAATTY | 1 cut(s) | 79 |
| Asp718I | GGTACC | 1 cut(s) | 252 |
| AspLEI | GCGC | 2 cut(s) | 363, 544 |
| AspS9I | GGNCC | 2 cut(s) | 35, 52 |
| AsuC2I | CCSGG | 2 cut(s) | 287, 467 |
| AsuHPI | GGTGA | 1 cut(s) | 31 |
| AvaI | CYCGRG | 1 cut(s) | 557 |
| AvaII | GGWCC | 2 cut(s) | 35, 52 |
| BaeGI | GKGCMC | 1 cut(s) | 389 |
| BanI | GGYRCC | 1 cut(s) | 252 |
| BbsI | GAAGAC | 1 cut(s) | 29 |
| Bbv12I | GWGCWC | 1 cut(s) | 528 |
| BbvI | GCAGC | 2 cut(s) | 349, 361 |
| BccI | CCATC | 1 cut(s) | 10 |
| BciT130I | CCWGG | 1 cut(s) | 56 |
| BcnI | CCSGG | 2 cut(s) | 287, 467 |
| BfaI | CTAG | 1 cut(s) | 368 |
| BfmI | CTRYAG | 1 cut(s) | 335 |
| BisI | GCNGC | 6 cut(s) | 9, 169, 259, 338, 375, 554 |
| BlpI | GCTNAGC | 1 cut(s) | 420 |
| BlsI | GCNGC | 6 cut(s) | 10, 170, 260, 339, 376, 555 |
| Bme1390I | CCNGG | 3 cut(s) | 56, 287, 467 |
| Bme18I | GGWCC | 2 cut(s) | 35, 52 |
| BmeT110I | CYCGRG | 1 cut(s) | 557 |
| BmgBI | CACGTC | 1 cut(s) | 504 |
| BmgT120I | GGNCC | 2 cut(s) | 35, 52 |
| BmiI | GGNNCC | 2 cut(s) | 37, 254 |
| BmrFI | CCNGG | 3 cut(s) | 56, 287, 467 |
| BpiI | GAAGAC | 1 cut(s) | 29 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 420 |
| BpuMI | CCSGG | 2 cut(s) | 287, 467 |
| BsaAI | YACGTR | 1 cut(s) | 523 |
| BsaJI | CCNNGG | 3 cut(s) | 107, 172, 286 |
| BsaWI | WCCGGW | 2 cut(s) | 49, 249 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 327 |
| BseBI | CCWGG | 1 cut(s) | 56 |
| BseDI | CCNNGG | 3 cut(s) | 107, 172, 286 |
| BseGI | GGATG | 1 cut(s) | 117 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 327 |
| BseRI | GAGGAG | 2 cut(s) | 206, 497 |
| BseSI | GKGCMC | 1 cut(s) | 389 |
| BseXI | GCAGC | 2 cut(s) | 349, 361 |
| Bsh1236I | CGCG | 3 cut(s) | 109, 363, 533 |
| Bsh1285I | CGRYCG | 2 cut(s) | 50, 551 |
| BshFI | GGCC | 1 cut(s) | 171 |
| BshNI | GGYRCC | 1 cut(s) | 252 |
| BsiEI | CGRYCG | 2 cut(s) | 50, 551 |
| BsiHKAI | GWGCWC | 1 cut(s) | 528 |
| BsiHKCI | CYCGRG | 1 cut(s) | 557 |
| BsiSI | CCGG | 4 cut(s) | 50, 250, 286, 467 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 327 |
| BsmI | GAATGC | 1 cut(s) | 133 |
| BsnI | GGCC | 1 cut(s) | 171 |
| BsoBI | CYCGRG | 1 cut(s) | 557 |
| Bsp1286I | GDGCHC | 2 cut(s) | 389, 528 |
| Bsp143I | GATC | 4 cut(s) | 3, 379, 390, 495 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 420 |
| BspANI | GGCC | 1 cut(s) | 171 |
| BspFNI | CGCG | 3 cut(s) | 109, 363, 533 |
| BspLI | GGNNCC | 2 cut(s) | 37, 254 |
| BspMAI | CTGCAG | 1 cut(s) | 339 |
| BspPI | GGATC | 1 cut(s) | 387 |
| BspQI | GCTCTTC | 1 cut(s) | 322 |
| BspT107I | GGYRCC | 1 cut(s) | 252 |
| BsrBI | CCGCTC | 2 cut(s) | 168, 556 |
| BssECI | CCNNGG | 3 cut(s) | 107, 172, 286 |
| BssMI | GATC | 4 cut(s) | 3, 379, 390, 495 |
| Bst2UI | CCWGG | 1 cut(s) | 56 |
| Bst4CI | ACNGT | 2 cut(s) | 176, 549 |
| Bst6I | CTCTTC | 1 cut(s) | 322 |
| BstBAI | YACGTR | 1 cut(s) | 523 |
| BstC8I | GCNNGC | 2 cut(s) | 245, 365 |
| BstDEI | CTNAG | 1 cut(s) | 420 |
| BstDSI | CCRYGG | 2 cut(s) | 107, 172 |
| BstF5I | GGATG | 1 cut(s) | 117 |
| BstFNI | CGCG | 3 cut(s) | 109, 363, 533 |
| BstHHI | GCGC | 2 cut(s) | 363, 544 |
| BstKTI | GATC | 4 cut(s) | 6, 382, 393, 498 |
| BstMBI | GATC | 4 cut(s) | 3, 379, 390, 495 |
| BstMCI | CGRYCG | 2 cut(s) | 50, 551 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 168, 177, 539, 550 |
| BstNI | CCWGG | 1 cut(s) | 56 |
| BstSCI | CCNGG | 3 cut(s) | 54, 285, 465 |
| BstSFI | CTRYAG | 1 cut(s) | 335 |
| BstSLI | GKGCMC | 1 cut(s) | 389 |
| BstUI | CGCG | 3 cut(s) | 109, 363, 533 |
| BstV1I | GCAGC | 2 cut(s) | 349, 361 |
| BstV2I | GAAGAC | 1 cut(s) | 29 |
| BsuRI | GGCC | 1 cut(s) | 171 |
| BtgI | CCRYGG | 2 cut(s) | 107, 172 |
| BtrI | CACGTC | 1 cut(s) | 504 |
| BtsCI | GGATG | 1 cut(s) | 117 |
| Cac8I | GCNNGC | 2 cut(s) | 245, 365 |
| CfoI | GCGC | 2 cut(s) | 363, 544 |
| Cfr13I | GGNCC | 2 cut(s) | 35, 52 |
| Cfr42I | CCGCGG | 1 cut(s) | 110 |
| CseI | GACGC | 1 cut(s) | 555 |
| Csp6I | GTAC | 2 cut(s) | 253, 281 |
| CviAII | CATG | 1 cut(s) | 514 |
| CviQI | GTAC | 2 cut(s) | 253, 281 |
| DdeI | CTNAG | 1 cut(s) | 420 |
| DpnI | GATC | 4 cut(s) | 5, 381, 392, 497 |
| DpnII | GATC | 4 cut(s) | 3, 379, 390, 495 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 443 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 443 |
| EaeI | YGGCCR | 1 cut(s) | 169 |
| Eam1104I | CTCTTC | 1 cut(s) | 322 |
| EarI | CTCTTC | 1 cut(s) | 322 |
| EciI | GGCGGA | 1 cut(s) | 230 |
| Eco47I | GGWCC | 2 cut(s) | 35, 52 |
| Eco88I | CYCGRG | 1 cut(s) | 557 |
| EcoRII | CCWGG | 1 cut(s) | 54 |
| FaeI | CATG | 1 cut(s) | 517 |
| FaiI | YATR | 5 cut(s) | 236, 479, 494, 515, 540 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 14, 46 |
| FatI | CATG | 1 cut(s) | 513 |
| FauI | CCCGC | 1 cut(s) | 538 |
| Fnu4HI | GCNGC | 6 cut(s) | 9, 169, 259, 338, 375, 554 |
| FokI | GGATG | 1 cut(s) | 124 |
| Fsp4HI | GCNGC | 6 cut(s) | 9, 169, 259, 338, 375, 554 |
| FspBI | CTAG | 1 cut(s) | 368 |
| GlaI | GCGC | 2 cut(s) | 362, 543 |
| GluI | GCNGC | 6 cut(s) | 9, 169, 259, 338, 375, 554 |
| HaeIII | GGCC | 1 cut(s) | 171 |
| HapII | CCGG | 4 cut(s) | 50, 250, 286, 467 |
| HgaI | GACGC | 1 cut(s) | 555 |
| HhaI | GCGC | 2 cut(s) | 363, 544 |
| Hin1II | CATG | 1 cut(s) | 517 |
| Hin6I | GCGC | 2 cut(s) | 361, 542 |
| HinP1I | GCGC | 2 cut(s) | 361, 542 |
| HincII | GTYRAC | 1 cut(s) | 448 |
| HindII | GTYRAC | 1 cut(s) | 448 |
| HindIII | AAGCTT | 1 cut(s) | 458 |
| HinfI | GANTC | 2 cut(s) | 96, 141 |
| HpaII | CCGG | 4 cut(s) | 50, 250, 286, 467 |
| HphI | GGTGA | 1 cut(s) | 31 |
| Hpy166II | GTNNAC | 3 cut(s) | 283, 448, 520 |
| Hpy188III | TCNNGA | 3 cut(s) | 138, 155, 559 |
| Hpy8I | GTNNAC | 3 cut(s) | 283, 448, 520 |
| Hpy99I | CGWCG | 1 cut(s) | 571 |
| HpyAV | CCTTC | 5 cut(s) | 152, 190, 264, 292, 408 |
| HpyCH4III | ACNGT | 2 cut(s) | 176, 549 |
| HpyCH4IV | ACGT | 2 cut(s) | 503, 522 |
| HpyCH4V | TGCA | 1 cut(s) | 337 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 168, 177, 539, 550 |
| HpyF3I | CTNAG | 1 cut(s) | 420 |
| HpySE526I | ACGT | 2 cut(s) | 503, 522 |
| Hsp92II | CATG | 1 cut(s) | 517 |
| HspAI | GCGC | 2 cut(s) | 361, 542 |
| KpnI | GGTACC | 1 cut(s) | 256 |
| KspI | CCGCGG | 1 cut(s) | 110 |
| Kzo9I | GATC | 4 cut(s) | 3, 379, 390, 495 |
| LguI | GCTCTTC | 1 cut(s) | 322 |
| LmnI | GCTCC | 2 cut(s) | 252, 531 |
| LpnPI | CCDG | 7 cut(s) | 41, 63, 68, 257, 263, 299, 480 |
| Lsp1109I | GCAGC | 2 cut(s) | 349, 361 |
| MaeI | CTAG | 1 cut(s) | 368 |
| MaeII | ACGT | 2 cut(s) | 503, 522 |
| MaeIII | GTNAC | 1 cut(s) | 509 |
| MalI | GATC | 4 cut(s) | 5, 381, 392, 497 |
| MbiI | CCGCTC | 2 cut(s) | 168, 556 |
| MboI | GATC | 4 cut(s) | 3, 379, 390, 495 |
| MboII | GAAGA | 4 cut(s) | 34, 79, 309, 482 |
| MfeI | CAATTG | 1 cut(s) | 404 |
| MhlI | GDGCHC | 2 cut(s) | 389, 528 |
| MluCI | AATT | 4 cut(s) | 79, 146, 404, 425 |
| MlyI | GAGTC | 1 cut(s) | 135 |
| MnlI | CCTC | 6 cut(s) | 54, 184, 321, 364, 475, 528 |
| MspA1I | CMGCKG | 1 cut(s) | 109 |
| MspI | CCGG | 4 cut(s) | 50, 250, 286, 467 |
| MspR9I | CCNGG | 3 cut(s) | 56, 287, 467 |
| MunI | CAATTG | 1 cut(s) | 404 |
| Mva1269I | GAATGC | 1 cut(s) | 133 |
| MvaI | CCWGG | 1 cut(s) | 56 |
| MvnI | CGCG | 3 cut(s) | 109, 363, 533 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 168, 177, 539, 550 |
| NciI | CCSGG | 2 cut(s) | 287, 467 |
| NdeII | GATC | 4 cut(s) | 3, 379, 390, 495 |
| NlaIII | CATG | 1 cut(s) | 517 |
| NlaIV | GGNNCC | 2 cut(s) | 37, 254 |
| PciSI | GCTCTTC | 1 cut(s) | 322 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 407 |
| PctI | GAATGC | 1 cut(s) | 133 |
| PfeI | GAWTC | 1 cut(s) | 96 |
| PflFI | GACNNNGTC | 1 cut(s) | 565 |
| PkrI | GCNGC | 6 cut(s) | 10, 170, 260, 339, 376, 555 |
| PleI | GAGTC | 1 cut(s) | 135 |
| PpsI | GAGTC | 1 cut(s) | 135 |
| Ppu21I | YACGTR | 1 cut(s) | 523 |
| Psp6I | CCWGG | 1 cut(s) | 54 |
| PspGI | CCWGG | 1 cut(s) | 54 |
| PspN4I | GGNNCC | 2 cut(s) | 37, 254 |
| PspPI | GGNCC | 2 cut(s) | 35, 52 |
| PstI | CTGCAG | 1 cut(s) | 339 |
| PsyI | GACNNNGTC | 1 cut(s) | 565 |
| RsaI | GTAC | 2 cut(s) | 254, 282 |
| RsaNI | GTAC | 2 cut(s) | 253, 281 |
| SacII | CCGCGG | 1 cut(s) | 110 |
| SapI | GCTCTTC | 1 cut(s) | 322 |
| SatI | GCNGC | 6 cut(s) | 9, 169, 259, 338, 375, 554 |
| Sau3AI | GATC | 4 cut(s) | 3, 379, 390, 495 |
| Sau96I | GGNCC | 2 cut(s) | 35, 52 |
| SchI | GAGTC | 1 cut(s) | 135 |
| ScrFI | CCNGG | 3 cut(s) | 56, 287, 467 |
| SduI | GDGCHC | 2 cut(s) | 389, 528 |
| SetI | ASST | 7 cut(s) | 124, 317, 347, 462, 506, 525, 539 |
| SfcI | CTRYAG | 1 cut(s) | 335 |
| Sfr303I | CCGCGG | 1 cut(s) | 110 |
| SgrBI | CCGCGG | 1 cut(s) | 110 |
| SinI | GGWCC | 2 cut(s) | 35, 52 |
| Sse9I | AATT | 4 cut(s) | 79, 146, 404, 425 |
| SspMI | CTAG | 1 cut(s) | 368 |
| StyD4I | CCNGG | 3 cut(s) | 54, 285, 465 |
| TaaI | ACNGT | 2 cut(s) | 176, 549 |
| TaiI | ACGT | 2 cut(s) | 506, 525 |
| TaqI | TCGA | 4 cut(s) | 46, 182, 223, 498 |
| TaqII | GACCGA | 1 cut(s) | 41 |
| TasI | AATT | 4 cut(s) | 79, 146, 404, 425 |
| TauI | GCSGC | 4 cut(s) | 11, 171, 261, 556 |
| TfiI | GAWTC | 1 cut(s) | 96 |
| TseI | GCWGC | 2 cut(s) | 337, 374 |
| Tth111I | GACNNNGTC | 1 cut(s) | 565 |
| VpaK11BI | GGWCC | 2 cut(s) | 35, 52 |
| XapI | RAATTY | 1 cut(s) | 79 |
| XspI | CTAG | 1 cut(s) | 368 |