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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 256, 419 |
| AccII | CGCG | 1 cut(s) | 449 |
| AciI | CCGC | 2 cut(s) | 256, 417 |
| AfaI | GTAC | 1 cut(s) | 114 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 149 |
| AjnI | CCWGG | 1 cut(s) | 372 |
| AleI | CACNNNNGTG | 1 cut(s) | 161 |
| AluBI | AGCT | 1 cut(s) | 391 |
| AluI | AGCT | 1 cut(s) | 391 |
| Alw21I | GWGCWC | 1 cut(s) | 57 |
| AoxI | GGCC | 2 cut(s) | 309, 414 |
| ApeKI | GCWGC | 2 cut(s) | 122, 284 |
| AspLEI | GCGC | 1 cut(s) | 432 |
| AspS9I | GGNCC | 1 cut(s) | 310 |
| BaeGI | GKGCMC | 1 cut(s) | 222 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 96, 129 |
| BauI | CACGAG | 1 cut(s) | 161 |
| Bbv12I | GWGCWC | 1 cut(s) | 57 |
| BbvI | GCAGC | 2 cut(s) | 109, 271 |
| BccI | CCATC | 2 cut(s) | 272, 295 |
| BceAI | ACGGC | 1 cut(s) | 84 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 34, 68 |
| BciT130I | CCWGG | 1 cut(s) | 374 |
| BclI | TGATCA | 1 cut(s) | 76 |
| BfaI | CTAG | 1 cut(s) | 38 |
| BisI | GCNGC | 4 cut(s) | 123, 257, 285, 417 |
| BlsI | GCNGC | 4 cut(s) | 124, 258, 286, 418 |
| Bme1390I | CCNGG | 1 cut(s) | 374 |
| BmgT120I | GGNCC | 1 cut(s) | 310 |
| BmrFI | CCNGG | 1 cut(s) | 374 |
| BmsI | GCATC | 2 cut(s) | 441, 479 |
| BpmI | CTGGAG | 1 cut(s) | 126 |
| BsaJI | CCNNGG | 1 cut(s) | 66 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 124, 154 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 149 |
| Bse1I | ACTGG | 1 cut(s) | 143 |
| BseBI | CCWGG | 1 cut(s) | 374 |
| BseDI | CCNNGG | 1 cut(s) | 66 |
| BseGI | GGATG | 2 cut(s) | 62, 306 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 149 |
| BseMII | CTCAG | 1 cut(s) | 434 |
| BseNI | ACTGG | 1 cut(s) | 143 |
| BseRI | GAGGAG | 2 cut(s) | 49, 266 |
| BseSI | GKGCMC | 1 cut(s) | 222 |
| BseXI | GCAGC | 2 cut(s) | 109, 271 |
| Bsh1236I | CGCG | 1 cut(s) | 449 |
| Bsh1285I | CGRYCG | 1 cut(s) | 111 |
| BshFI | GGCC | 2 cut(s) | 311, 416 |
| BsiEI | CGRYCG | 1 cut(s) | 111 |
| BsiHKAI | GWGCWC | 1 cut(s) | 57 |
| BslFI | GGGAC | 2 cut(s) | 214, 357 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 149 |
| BsmFI | GGGAC | 2 cut(s) | 214, 357 |
| BsmI | GAATGC | 1 cut(s) | 244 |
| BsnI | GGCC | 2 cut(s) | 311, 416 |
| Bsp1286I | GDGCHC | 2 cut(s) | 57, 222 |
| Bsp143I | GATC | 2 cut(s) | 76, 409 |
| Bsp68I | TCGCGA | 1 cut(s) | 449 |
| BspACI | CCGC | 2 cut(s) | 256, 417 |
| BspANI | GGCC | 2 cut(s) | 311, 416 |
| BspCNI | CTCAG | 1 cut(s) | 433 |
| BspFNI | CGCG | 1 cut(s) | 449 |
| BsrBI | CCGCTC | 2 cut(s) | 256, 419 |
| BsrI | ACTGG | 1 cut(s) | 143 |
| BssECI | CCNNGG | 1 cut(s) | 66 |
| BssMI | GATC | 2 cut(s) | 76, 409 |
| BssSI | CACGAG | 1 cut(s) | 161 |
| Bst2BI | CACGAG | 1 cut(s) | 161 |
| Bst2UI | CCWGG | 1 cut(s) | 374 |
| Bst4CI | ACNGT | 4 cut(s) | 112, 172, 194, 348 |
| Bst6I | CTCTTC | 1 cut(s) | 315 |
| BstC8I | GCNNGC | 3 cut(s) | 244, 261, 428 |
| BstDEI | CTNAG | 1 cut(s) | 420 |
| BstDSI | CCRYGG | 1 cut(s) | 66 |
| BstF5I | GGATG | 2 cut(s) | 62, 306 |
| BstFNI | CGCG | 1 cut(s) | 449 |
| BstHHI | GCGC | 1 cut(s) | 432 |
| BstKTI | GATC | 2 cut(s) | 79, 412 |
| BstMBI | GATC | 2 cut(s) | 76, 409 |
| BstMCI | CGRYCG | 1 cut(s) | 111 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 446, 455 |
| BstNI | CCWGG | 1 cut(s) | 374 |
| BstSCI | CCNGG | 1 cut(s) | 372 |
| BstSLI | GKGCMC | 1 cut(s) | 222 |
| BstUI | CGCG | 1 cut(s) | 449 |
| BstV1I | GCAGC | 2 cut(s) | 109, 271 |
| BsuRI | GGCC | 2 cut(s) | 311, 416 |
| BtgI | CCRYGG | 1 cut(s) | 66 |
| BtgZI | GCGATG | 1 cut(s) | 430 |
| BtsCI | GGATG | 2 cut(s) | 62, 306 |
| BtsI | GCAGTG | 1 cut(s) | 489 |
| BtsIMutI | CAGTG | 3 cut(s) | 199, 353, 489 |
| BtuMI | TCGCGA | 1 cut(s) | 449 |
| Cac8I | GCNNGC | 3 cut(s) | 244, 261, 428 |
| CfoI | GCGC | 1 cut(s) | 432 |
| Cfr13I | GGNCC | 1 cut(s) | 310 |
| Csp6I | GTAC | 1 cut(s) | 113 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 55, 90 |
| CviJI | RGCY | 8 cut(s) | 153, 259, 311, 334, 372, 391, 416, 458 |
| CviKI_1 | RGCY | 8 cut(s) | 153, 259, 311, 334, 372, 391, 416, 458 |
| CviQI | GTAC | 1 cut(s) | 113 |
| DdeI | CTNAG | 1 cut(s) | 420 |
| DpnI | GATC | 2 cut(s) | 78, 411 |
| DpnII | GATC | 2 cut(s) | 76, 409 |
| Eam1104I | CTCTTC | 1 cut(s) | 315 |
| EarI | CTCTTC | 1 cut(s) | 315 |
| EcoRII | CCWGG | 1 cut(s) | 372 |
| FaiI | YATR | 6 cut(s) | 26, 289, 291, 361, 401, 465 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 27, 85, 117 |
| FaqI | GGGAC | 2 cut(s) | 214, 357 |
| FbaI | TGATCA | 1 cut(s) | 76 |
| Fnu4HI | GCNGC | 4 cut(s) | 123, 257, 285, 417 |
| FokI | GGATG | 2 cut(s) | 49, 313 |
| Fsp4HI | GCNGC | 4 cut(s) | 123, 257, 285, 417 |
| FspBI | CTAG | 1 cut(s) | 38 |
| GlaI | GCGC | 1 cut(s) | 431 |
| GluI | GCNGC | 4 cut(s) | 123, 257, 285, 417 |
| GsuI | CTGGAG | 1 cut(s) | 126 |
| HaeIII | GGCC | 2 cut(s) | 311, 416 |
| HhaI | GCGC | 1 cut(s) | 432 |
| Hin6I | GCGC | 1 cut(s) | 430 |
| HinP1I | GCGC | 1 cut(s) | 430 |
| HinfI | GANTC | 2 cut(s) | 98, 209 |
| Hpy188I | TCNGA | 3 cut(s) | 18, 423, 475 |
| Hpy188III | TCNNGA | 2 cut(s) | 80, 448 |
| HpyAV | CCTTC | 2 cut(s) | 191, 324 |
| HpyCH4III | ACNGT | 4 cut(s) | 112, 172, 194, 348 |
| HpyCH4V | TGCA | 2 cut(s) | 287, 470 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 446, 455 |
| HpyF3I | CTNAG | 1 cut(s) | 420 |
| HspAI | GCGC | 1 cut(s) | 430 |
| Ksp22I | TGATCA | 1 cut(s) | 76 |
| Kzo9I | GATC | 2 cut(s) | 76, 409 |
| LmnI | GCTCC | 1 cut(s) | 253 |
| LpnPI | CCDG | 5 cut(s) | 135, 156, 359, 386, 398 |
| Lsp1109I | GCAGC | 2 cut(s) | 109, 271 |
| LweI | GCATC | 2 cut(s) | 441, 479 |
| MaeI | CTAG | 1 cut(s) | 38 |
| MaeIII | GTNAC | 2 cut(s) | 40, 145 |
| MalI | GATC | 2 cut(s) | 78, 411 |
| MbiI | CCGCTC | 2 cut(s) | 256, 419 |
| MboI | GATC | 2 cut(s) | 76, 409 |
| MboII | GAAGA | 2 cut(s) | 43, 332 |
| MfeI | CAATTG | 1 cut(s) | 204 |
| MhlI | GDGCHC | 2 cut(s) | 57, 222 |
| MluCI | AATT | 2 cut(s) | 204, 476 |
| MnlI | CCTC | 7 cut(s) | 39, 70, 150, 244, 291, 323, 334 |
| MslI | CAYNNNNRTG | 1 cut(s) | 161 |
| MspR9I | CCNGG | 1 cut(s) | 374 |
| MunI | CAATTG | 1 cut(s) | 204 |
| Mva1269I | GAATGC | 1 cut(s) | 244 |
| MvaI | CCWGG | 1 cut(s) | 374 |
| MvnI | CGCG | 1 cut(s) | 449 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 446, 455 |
| NdeII | GATC | 2 cut(s) | 76, 409 |
| NmuCI | GTSAC | 1 cut(s) | 40 |
| NruI | TCGCGA | 1 cut(s) | 449 |
| OliI | CACNNNNGTG | 1 cut(s) | 161 |
| PctI | GAATGC | 1 cut(s) | 244 |
| PfeI | GAWTC | 2 cut(s) | 98, 209 |
| PkrI | GCNGC | 4 cut(s) | 124, 258, 286, 418 |
| Psp6I | CCWGG | 1 cut(s) | 372 |
| PspGI | CCWGG | 1 cut(s) | 372 |
| PspPI | GGNCC | 1 cut(s) | 310 |
| RruI | TCGCGA | 1 cut(s) | 449 |
| RsaI | GTAC | 1 cut(s) | 114 |
| RsaNI | GTAC | 1 cut(s) | 113 |
| RseI | CAYNNNNRTG | 1 cut(s) | 161 |
| SatI | GCNGC | 4 cut(s) | 123, 257, 285, 417 |
| Sau3AI | GATC | 2 cut(s) | 76, 409 |
| Sau96I | GGNCC | 1 cut(s) | 310 |
| ScrFI | CCNGG | 1 cut(s) | 374 |
| SduI | GDGCHC | 2 cut(s) | 57, 222 |
| SetI | ASST | 4 cut(s) | 39, 50, 142, 393 |
| SfaNI | GCATC | 2 cut(s) | 441, 479 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 161 |
| Sse9I | AATT | 2 cut(s) | 204, 476 |
| SsiI | CCGC | 2 cut(s) | 256, 417 |
| SspMI | CTAG | 1 cut(s) | 38 |
| StyD4I | CCNGG | 1 cut(s) | 372 |
| TaaI | ACNGT | 4 cut(s) | 112, 172, 194, 348 |
| TaqII | GACCGA | 1 cut(s) | 422 |
| TasI | AATT | 2 cut(s) | 204, 476 |
| TauI | GCSGC | 2 cut(s) | 259, 419 |
| TfiI | GAWTC | 2 cut(s) | 98, 209 |
| TscAI | CASTG | 3 cut(s) | 199, 353, 489 |
| TseFI | GTSAC | 1 cut(s) | 40 |
| TseI | GCWGC | 2 cut(s) | 122, 284 |
| Tsp45I | GTSAC | 1 cut(s) | 40 |
| TspDTI | ATGAA | 1 cut(s) | 203 |
| TspGWI | ACGGA | 1 cut(s) | 332 |
| TspRI | CASTG | 3 cut(s) | 199, 353, 489 |
| XspI | CTAG | 1 cut(s) | 38 |