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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 86, 131, 332 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 686 |
| AccBSI | CCGCTC | 1 cut(s) | 487 |
| AccII | CGCG | 1 cut(s) | 115 |
| AcoI | YGGCCR | 1 cut(s) | 271 |
| AcyI | GRCGYC | 1 cut(s) | 87 |
| AdeI | CACNNNGTG | 1 cut(s) | 622 |
| AfaI | GTAC | 2 cut(s) | 296, 300 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 686 |
| AgsI | TTSAA | 1 cut(s) | 229 |
| AluBI | AGCT | 4 cut(s) | 236, 389, 475, 518 |
| AluI | AGCT | 4 cut(s) | 236, 389, 475, 518 |
| Alw26I | GTCTC | 1 cut(s) | 237 |
| AoxI | GGCC | 4 cut(s) | 163, 271, 458, 712 |
| ApeKI | GCWGC | 6 cut(s) | 4, 176, 359, 389, 475, 507 |
| AspLEI | GCGC | 1 cut(s) | 89 |
| AspS9I | GGNCC | 2 cut(s) | 164, 713 |
| AsuC2I | CCSGG | 3 cut(s) | 212, 512, 584 |
| AsuHPI | GGTGA | 2 cut(s) | 35, 601 |
| BalI | TGGCCA | 1 cut(s) | 273 |
| BanI | GGYRCC | 3 cut(s) | 86, 131, 332 |
| BanII | GRGCYC | 1 cut(s) | 54 |
| BbsI | GAAGAC | 1 cut(s) | 143 |
| BbvI | GCAGC | 6 cut(s) | 16, 163, 346, 376, 462, 494 |
| BccI | CCATC | 2 cut(s) | 118, 155 |
| BciVI | GTATCC | 1 cut(s) | 3 |
| BcnI | CCSGG | 3 cut(s) | 212, 512, 584 |
| BcoDI | GTCTC | 1 cut(s) | 237 |
| BfoI | RGCGCY | 1 cut(s) | 90 |
| BfuI | GTATCC | 1 cut(s) | 3 |
| BisI | GCNGC | 7 cut(s) | 5, 177, 336, 360, 390, 476, 508 |
| BlsI | GCNGC | 7 cut(s) | 6, 178, 337, 361, 391, 477, 509 |
| Bme1390I | CCNGG | 3 cut(s) | 212, 512, 584 |
| BmgT120I | GGNCC | 2 cut(s) | 164, 713 |
| BmiI | GGNNCC | 5 cut(s) | 88, 133, 265, 334, 715 |
| BmrFI | CCNGG | 3 cut(s) | 212, 512, 584 |
| BmsI | GCATC | 3 cut(s) | 96, 434, 461 |
| BpiI | GAAGAC | 1 cut(s) | 143 |
| BpuMI | CCSGG | 3 cut(s) | 212, 512, 584 |
| BsaAI | YACGTR | 1 cut(s) | 298 |
| BsaHI | GRCGYC | 1 cut(s) | 87 |
| BsaI | GGTCTC | 1 cut(s) | 237 |
| BsaJI | CCNNGG | 4 cut(s) | 46, 259, 268, 561 |
| BsaWI | WCCGGW | 2 cut(s) | 281, 500 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 686 |
| Bse118I | RCCGGY | 2 cut(s) | 604, 757 |
| Bse1I | ACTGG | 1 cut(s) | 534 |
| BseDI | CCNNGG | 4 cut(s) | 46, 259, 268, 561 |
| BseGI | GGATG | 2 cut(s) | 111, 166 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 686 |
| BseNI | ACTGG | 1 cut(s) | 534 |
| BseRI | GAGGAG | 3 cut(s) | 691, 694, 697 |
| BseXI | GCAGC | 6 cut(s) | 16, 163, 346, 376, 462, 494 |
| BseYI | CCCAGC | 1 cut(s) | 665 |
| Bsh1236I | CGCG | 1 cut(s) | 115 |
| BshFI | GGCC | 4 cut(s) | 165, 273, 460, 714 |
| BshNI | GGYRCC | 3 cut(s) | 86, 131, 332 |
| BsiSI | CCGG | 8 cut(s) | 211, 282, 501, 511, 584, 605, 711, 758 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 686 |
| BsmAI | GTCTC | 1 cut(s) | 237 |
| BsnI | GGCC | 4 cut(s) | 165, 273, 460, 714 |
| Bso31I | GGTCTC | 1 cut(s) | 237 |
| Bsp1286I | GDGCHC | 1 cut(s) | 54 |
| Bsp143I | GATC | 2 cut(s) | 98, 750 |
| Bsp68I | TCGCGA | 1 cut(s) | 115 |
| BspANI | GGCC | 4 cut(s) | 165, 273, 460, 714 |
| BspFNI | CGCG | 1 cut(s) | 115 |
| BspLI | GGNNCC | 5 cut(s) | 88, 133, 265, 334, 715 |
| BspT107I | GGYRCC | 3 cut(s) | 86, 131, 332 |
| BspTNI | GGTCTC | 1 cut(s) | 237 |
| BsrBI | CCGCTC | 1 cut(s) | 487 |
| BsrFI | RCCGGY | 2 cut(s) | 604, 757 |
| BsrI | ACTGG | 1 cut(s) | 534 |
| BssAI | RCCGGY | 2 cut(s) | 604, 757 |
| BssECI | CCNNGG | 4 cut(s) | 46, 259, 268, 561 |
| BssMI | GATC | 2 cut(s) | 98, 750 |
| BssNI | GRCGYC | 1 cut(s) | 87 |
| BssT1I | CCWWGG | 3 cut(s) | 259, 268, 561 |
| Bst4CI | ACNGT | 3 cut(s) | 279, 332, 780 |
| Bst6I | CTCTTC | 2 cut(s) | 729, 738 |
| BstACI | GRCGYC | 1 cut(s) | 87 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 131 |
| BstBAI | YACGTR | 1 cut(s) | 298 |
| BstC8I | GCNNGC | 3 cut(s) | 127, 677, 759 |
| BstEII | GGTNACC | 1 cut(s) | 539 |
| BstF5I | GGATG | 2 cut(s) | 111, 166 |
| BstFNI | CGCG | 1 cut(s) | 115 |
| BstH2I | RGCGCY | 1 cut(s) | 90 |
| BstHHI | GCGC | 1 cut(s) | 89 |
| BstKTI | GATC | 2 cut(s) | 101, 753 |
| BstMAI | GTCTC | 1 cut(s) | 237 |
| BstMBI | GATC | 2 cut(s) | 98, 750 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 131, 162, 270, 356, 484, 493 |
| BstNSI | RCATGY | 1 cut(s) | 629 |
| BstPI | GGTNACC | 1 cut(s) | 539 |
| BstSCI | CCNGG | 3 cut(s) | 210, 510, 582 |
| BstSNI | TACGTA | 1 cut(s) | 298 |
| BstUI | CGCG | 1 cut(s) | 115 |
| BstV1I | GCAGC | 6 cut(s) | 16, 163, 346, 376, 462, 494 |
| BstV2I | GAAGAC | 1 cut(s) | 143 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 66 |
| BsuI | GTATCC | 1 cut(s) | 3 |
| BsuRI | GGCC | 4 cut(s) | 165, 273, 460, 714 |
| BtgZI | GCGATG | 1 cut(s) | 96 |
| BtsCI | GGATG | 2 cut(s) | 111, 166 |
| BtsIMutI | CAGTG | 1 cut(s) | 541 |
| BtuMI | TCGCGA | 1 cut(s) | 115 |
| Cac8I | GCNNGC | 3 cut(s) | 127, 677, 759 |
| CfoI | GCGC | 1 cut(s) | 89 |
| Cfr10I | RCCGGY | 2 cut(s) | 604, 757 |
| Cfr13I | GGNCC | 2 cut(s) | 164, 713 |
| Csp6I | GTAC | 2 cut(s) | 295, 299 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 450, 485 |
| CviAII | CATG | 5 cut(s) | 55, 66, 102, 626, 681 |
| CviQI | GTAC | 2 cut(s) | 295, 299 |
| DinI | GGCGCC | 1 cut(s) | 88 |
| DpnI | GATC | 2 cut(s) | 100, 752 |
| DpnII | GATC | 2 cut(s) | 98, 750 |
| DraIII | CACNNNGTG | 1 cut(s) | 622 |
| EaeI | YGGCCR | 1 cut(s) | 271 |
| Eam1104I | CTCTTC | 2 cut(s) | 729, 738 |
| EarI | CTCTTC | 2 cut(s) | 729, 738 |
| Eco105I | TACGTA | 1 cut(s) | 298 |
| Eco130I | CCWWGG | 3 cut(s) | 259, 268, 561 |
| Eco24I | GRGCYC | 1 cut(s) | 54 |
| Eco31I | GGTCTC | 1 cut(s) | 237 |
| Eco91I | GGTNACC | 1 cut(s) | 539 |
| EcoO65I | GGTNACC | 1 cut(s) | 539 |
| EcoT14I | CCWWGG | 3 cut(s) | 259, 268, 561 |
| EcoT38I | GRGCYC | 1 cut(s) | 54 |
| EgeI | GGCGCC | 1 cut(s) | 88 |
| EheI | GGCGCC | 1 cut(s) | 88 |
| ErhI | CCWWGG | 3 cut(s) | 259, 268, 561 |
| FaeI | CATG | 5 cut(s) | 58, 69, 105, 629, 684 |
| FatI | CATG | 5 cut(s) | 54, 65, 101, 625, 680 |
| FauI | CCCGC | 1 cut(s) | 139 |
| Fnu4HI | GCNGC | 7 cut(s) | 5, 177, 336, 360, 390, 476, 508 |
| FokI | GGATG | 2 cut(s) | 118, 173 |
| FriOI | GRGCYC | 1 cut(s) | 54 |
| Fsp4HI | GCNGC | 7 cut(s) | 5, 177, 336, 360, 390, 476, 508 |
| GlaI | GCGC | 1 cut(s) | 88 |
| GluI | GCNGC | 7 cut(s) | 5, 177, 336, 360, 390, 476, 508 |
| GsaI | CCCAGC | 1 cut(s) | 669 |
| HaeII | RGCGCY | 1 cut(s) | 90 |
| HaeIII | GGCC | 4 cut(s) | 165, 273, 460, 714 |
| HapII | CCGG | 8 cut(s) | 211, 282, 501, 511, 584, 605, 711, 758 |
| HhaI | GCGC | 1 cut(s) | 89 |
| Hin1I | GRCGYC | 1 cut(s) | 87 |
| Hin1II | CATG | 5 cut(s) | 58, 69, 105, 629, 684 |
| Hin6I | GCGC | 1 cut(s) | 87 |
| HinP1I | GCGC | 1 cut(s) | 87 |
| HindIII | AAGCTT | 1 cut(s) | 234 |
| HinfI | GANTC | 4 cut(s) | 81, 117, 307, 592 |
| HpaII | CCGG | 8 cut(s) | 211, 282, 501, 511, 584, 605, 711, 758 |
| HphI | GGTGA | 2 cut(s) | 35, 601 |
| Hpy166II | GTNNAC | 1 cut(s) | 652 |
| Hpy188I | TCNGA | 2 cut(s) | 36, 434 |
| Hpy188III | TCNNGA | 1 cut(s) | 114 |
| Hpy8I | GTNNAC | 1 cut(s) | 652 |
| Hpy99I | CGWCG | 3 cut(s) | 140, 484, 600 |
| HpyAV | CCTTC | 2 cut(s) | 313, 354 |
| HpyCH4III | ACNGT | 3 cut(s) | 279, 332, 780 |
| HpyCH4IV | ACGT | 2 cut(s) | 297, 618 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 625 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 131, 162, 270, 356, 484, 493 |
| HpySE526I | ACGT | 2 cut(s) | 297, 618 |
| Hsp92I | GRCGYC | 1 cut(s) | 87 |
| Hsp92II | CATG | 5 cut(s) | 58, 69, 105, 629, 684 |
| HspAI | GCGC | 1 cut(s) | 87 |
| KasI | GGCGCC | 1 cut(s) | 86 |
| KroI | GCCGGC | 1 cut(s) | 757 |
| KroNI | GCCGGC | 1 cut(s) | 759 |
| Kzo9I | GATC | 2 cut(s) | 98, 750 |
| LmnI | GCTCC | 3 cut(s) | 269, 472, 484 |
| Lsp1109I | GCAGC | 6 cut(s) | 16, 163, 346, 376, 462, 494 |
| LweI | GCATC | 3 cut(s) | 96, 434, 461 |
| MaeII | ACGT | 2 cut(s) | 297, 618 |
| MaeIII | GTNAC | 2 cut(s) | 539, 589 |
| MalI | GATC | 2 cut(s) | 100, 752 |
| MbiI | CCGCTC | 1 cut(s) | 487 |
| MboI | GATC | 2 cut(s) | 98, 750 |
| MboII | GAAGA | 3 cut(s) | 143, 746, 755 |
| MhlI | GDGCHC | 1 cut(s) | 54 |
| MlsI | TGGCCA | 1 cut(s) | 273 |
| MluCI | AATT | 1 cut(s) | 546 |
| MluNI | TGGCCA | 1 cut(s) | 273 |
| Mly113I | GGCGCC | 1 cut(s) | 87 |
| MlyI | GAGTC | 2 cut(s) | 90, 586 |
| MnlI | CCTC | 8 cut(s) | 41, 450, 497, 709, 712, 715, 718, 730 |
| Mox20I | TGGCCA | 1 cut(s) | 273 |
| MroNI | GCCGGC | 1 cut(s) | 757 |
| MscI | TGGCCA | 1 cut(s) | 273 |
| MseI | TTAA | 1 cut(s) | 549 |
| MslI | CAYNNNNRTG | 1 cut(s) | 64 |
| Msp20I | TGGCCA | 1 cut(s) | 273 |
| MspA1I | CMGCKG | 2 cut(s) | 7, 359 |
| MspI | CCGG | 8 cut(s) | 211, 282, 501, 511, 584, 605, 711, 758 |
| MspR9I | CCNGG | 3 cut(s) | 212, 512, 584 |
| MvnI | CGCG | 1 cut(s) | 115 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 131, 162, 270, 356, 484, 493 |
| NaeI | GCCGGC | 1 cut(s) | 759 |
| NarI | GGCGCC | 1 cut(s) | 87 |
| NciI | CCSGG | 3 cut(s) | 212, 512, 584 |
| NdeII | GATC | 2 cut(s) | 98, 750 |
| NgoMIV | GCCGGC | 1 cut(s) | 757 |
| NlaIII | CATG | 5 cut(s) | 58, 69, 105, 629, 684 |
| NlaIV | GGNNCC | 5 cut(s) | 88, 133, 265, 334, 715 |
| NmuCI | GTSAC | 1 cut(s) | 589 |
| NruI | TCGCGA | 1 cut(s) | 115 |
| NspI | RCATGY | 1 cut(s) | 629 |
| PdiI | GCCGGC | 1 cut(s) | 759 |
| PfeI | GAWTC | 2 cut(s) | 117, 307 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 686 |
| PkrI | GCNGC | 7 cut(s) | 6, 178, 337, 361, 391, 477, 509 |
| PleI | GAGTC | 2 cut(s) | 89, 586 |
| PluTI | GGCGCC | 1 cut(s) | 90 |
| PpsI | GAGTC | 2 cut(s) | 89, 586 |
| Ppu21I | YACGTR | 1 cut(s) | 298 |
| PspEI | GGTNACC | 1 cut(s) | 539 |
| PspFI | CCCAGC | 1 cut(s) | 665 |
| PspN4I | GGNNCC | 5 cut(s) | 88, 133, 265, 334, 715 |
| PspPI | GGNCC | 2 cut(s) | 164, 713 |
| RruI | TCGCGA | 1 cut(s) | 115 |
| RsaI | GTAC | 2 cut(s) | 296, 300 |
| RsaNI | GTAC | 2 cut(s) | 295, 299 |
| RseI | CAYNNNNRTG | 1 cut(s) | 64 |
| SaqAI | TTAA | 1 cut(s) | 549 |
| SatI | GCNGC | 7 cut(s) | 5, 177, 336, 360, 390, 476, 508 |
| Sau3AI | GATC | 2 cut(s) | 98, 750 |
| Sau96I | GGNCC | 2 cut(s) | 164, 713 |
| SchI | GAGTC | 2 cut(s) | 90, 586 |
| ScrFI | CCNGG | 3 cut(s) | 212, 512, 584 |
| SduI | GDGCHC | 1 cut(s) | 54 |
| SfaNI | GCATC | 3 cut(s) | 96, 434, 461 |
| SfoI | GGCGCC | 1 cut(s) | 88 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 64 |
| SnaBI | TACGTA | 1 cut(s) | 298 |
| Sse9I | AATT | 1 cut(s) | 546 |
| SspDI | GGCGCC | 1 cut(s) | 86 |
| StyD4I | CCNGG | 3 cut(s) | 210, 510, 582 |
| StyI | CCWWGG | 3 cut(s) | 259, 268, 561 |
| TaaI | ACNGT | 3 cut(s) | 279, 332, 780 |
| TaiI | ACGT | 2 cut(s) | 300, 621 |
| TaqI | TCGA | 2 cut(s) | 455, 753 |
| TasI | AATT | 1 cut(s) | 546 |
| TauI | GCSGC | 1 cut(s) | 338 |
| TfiI | GAWTC | 2 cut(s) | 117, 307 |
| Tru1I | TTAA | 1 cut(s) | 549 |
| Tru9I | TTAA | 1 cut(s) | 549 |
| TscAI | CASTG | 1 cut(s) | 541 |
| TseFI | GTSAC | 1 cut(s) | 589 |
| TseI | GCWGC | 6 cut(s) | 4, 176, 359, 389, 475, 507 |
| Tsp45I | GTSAC | 1 cut(s) | 589 |
| TspGWI | ACGGA | 2 cut(s) | 458, 497 |
| TspRI | CASTG | 1 cut(s) | 541 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 686 |
| XceI | RCATGY | 1 cut(s) | 629 |