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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 204 |
| AccI | GTMKAC | 1 cut(s) | 284 |
| AccII | CGCG | 1 cut(s) | 311 |
| AclWI | GGATC | 1 cut(s) | 137 |
| AcsI | RAATTY | 1 cut(s) | 305 |
| AcuI | CTGAAG | 1 cut(s) | 210 |
| AcyI | GRCGYC | 1 cut(s) | 201 |
| AflII | CTTAAG | 1 cut(s) | 278 |
| AjnI | CCWGG | 1 cut(s) | 91 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 290, 322 |
| AleI | CACNNNNGTG | 1 cut(s) | 20 |
| AluBI | AGCT | 5 cut(s) | 10, 123, 277, 301, 413 |
| AluI | AGCT | 5 cut(s) | 10, 123, 277, 301, 413 |
| Alw21I | GWGCWC | 1 cut(s) | 39 |
| Alw26I | GTCTC | 1 cut(s) | 208 |
| AlwI | GGATC | 1 cut(s) | 137 |
| AoxI | GGCC | 1 cut(s) | 389 |
| ApeKI | GCWGC | 1 cut(s) | 113 |
| ApoI | RAATTY | 1 cut(s) | 305 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 255, 287 |
| AspLEI | GCGC | 1 cut(s) | 340 |
| AspS9I | GGNCC | 1 cut(s) | 390 |
| AsuC2I | CCSGG | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 2 cut(s) | 209, 286 |
| AsuII | TTCGAA | 1 cut(s) | 213 |
| BauI | CACGAG | 1 cut(s) | 58 |
| Bbv12I | GWGCWC | 1 cut(s) | 39 |
| BbvI | GCAGC | 1 cut(s) | 100 |
| BccI | CCATC | 1 cut(s) | 125 |
| BceAI | ACGGC | 2 cut(s) | 303, 350 |
| BciT130I | CCWGG | 1 cut(s) | 93 |
| BclI | TGATCA | 1 cut(s) | 12 |
| BcnI | CCSGG | 1 cut(s) | 173 |
| BcoDI | GTCTC | 1 cut(s) | 208 |
| BfaI | CTAG | 1 cut(s) | 302 |
| BfrI | CTTAAG | 1 cut(s) | 278 |
| BglII | AGATCT | 1 cut(s) | 345 |
| BisI | GCNGC | 1 cut(s) | 114 |
| BlsI | GCNGC | 1 cut(s) | 115 |
| Bme1390I | CCNGG | 2 cut(s) | 93, 173 |
| BmgT120I | GGNCC | 1 cut(s) | 390 |
| BmiI | GGNNCC | 1 cut(s) | 453 |
| BmrFI | CCNGG | 2 cut(s) | 93, 173 |
| Bpu14I | TTCGAA | 1 cut(s) | 213 |
| BpuMI | CCSGG | 1 cut(s) | 173 |
| BsaHI | GRCGYC | 1 cut(s) | 201 |
| BsaJI | CCNNGG | 2 cut(s) | 91, 92 |
| BseBI | CCWGG | 1 cut(s) | 93 |
| BseDI | CCNNGG | 2 cut(s) | 91, 92 |
| BseMII | CTCAG | 2 cut(s) | 58, 271 |
| BseRI | GAGGAG | 1 cut(s) | 197 |
| BseXI | GCAGC | 1 cut(s) | 100 |
| Bsh1236I | CGCG | 1 cut(s) | 311 |
| BshFI | GGCC | 1 cut(s) | 391 |
| BsiHKAI | GWGCWC | 1 cut(s) | 39 |
| BsiSI | CCGG | 1 cut(s) | 173 |
| BslFI | GGGAC | 1 cut(s) | 74 |
| BsmAI | GTCTC | 1 cut(s) | 208 |
| BsmBI | CGTCTC | 1 cut(s) | 208 |
| BsmFI | GGGAC | 1 cut(s) | 74 |
| BsmI | GAATGC | 1 cut(s) | 290 |
| BsnI | GGCC | 1 cut(s) | 391 |
| Bsp119I | TTCGAA | 1 cut(s) | 213 |
| Bsp1286I | GDGCHC | 1 cut(s) | 39 |
| Bsp143I | GATC | 4 cut(s) | 12, 142, 345, 407 |
| Bsp68I | TCGCGA | 1 cut(s) | 311 |
| BspANI | GGCC | 1 cut(s) | 391 |
| BspCNI | CTCAG | 2 cut(s) | 57, 270 |
| BspFNI | CGCG | 1 cut(s) | 311 |
| BspLI | GGNNCC | 1 cut(s) | 453 |
| BspPI | GGATC | 1 cut(s) | 137 |
| BspQI | GCTCTTC | 1 cut(s) | 241 |
| BspT104I | TTCGAA | 1 cut(s) | 213 |
| BspTI | CTTAAG | 1 cut(s) | 278 |
| BssECI | CCNNGG | 2 cut(s) | 91, 92 |
| BssMI | GATC | 4 cut(s) | 12, 142, 345, 407 |
| BssNI | GRCGYC | 1 cut(s) | 201 |
| BssSI | CACGAG | 1 cut(s) | 58 |
| Bst2BI | CACGAG | 1 cut(s) | 58 |
| Bst2UI | CCWGG | 1 cut(s) | 93 |
| Bst4CI | ACNGT | 1 cut(s) | 249 |
| Bst6I | CTCTTC | 2 cut(s) | 156, 241 |
| BstACI | GRCGYC | 1 cut(s) | 201 |
| BstAFI | CTTAAG | 1 cut(s) | 278 |
| BstBI | TTCGAA | 1 cut(s) | 213 |
| BstC8I | GCNNGC | 1 cut(s) | 132 |
| BstDEI | CTNAG | 3 cut(s) | 44, 257, 349 |
| BstFNI | CGCG | 1 cut(s) | 311 |
| BstHHI | GCGC | 1 cut(s) | 340 |
| BstKTI | GATC | 4 cut(s) | 15, 145, 348, 410 |
| BstMAI | GTCTC | 1 cut(s) | 208 |
| BstMBI | GATC | 4 cut(s) | 12, 142, 345, 407 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 274 |
| BstNI | CCWGG | 1 cut(s) | 93 |
| BstSCI | CCNGG | 2 cut(s) | 91, 171 |
| BstUI | CGCG | 1 cut(s) | 311 |
| BstV1I | GCAGC | 1 cut(s) | 100 |
| BstX2I | RGATCY | 1 cut(s) | 345 |
| BstYI | RGATCY | 1 cut(s) | 345 |
| BsuRI | GGCC | 1 cut(s) | 391 |
| BtuMI | TCGCGA | 1 cut(s) | 311 |
| Cac8I | GCNNGC | 1 cut(s) | 132 |
| CfoI | GCGC | 1 cut(s) | 340 |
| Cfr13I | GGNCC | 1 cut(s) | 390 |
| CviAII | CATG | 1 cut(s) | 341 |
| DdeI | CTNAG | 3 cut(s) | 44, 257, 349 |
| DpnI | GATC | 4 cut(s) | 14, 144, 347, 409 |
| DpnII | GATC | 4 cut(s) | 12, 142, 345, 407 |
| Eam1104I | CTCTTC | 2 cut(s) | 156, 241 |
| EarI | CTCTTC | 2 cut(s) | 156, 241 |
| Eco57I | CTGAAG | 1 cut(s) | 210 |
| EcoRI | GAATTC | 1 cut(s) | 305 |
| EcoRII | CCWGG | 1 cut(s) | 91 |
| Esp3I | CGTCTC | 1 cut(s) | 208 |
| FaeI | CATG | 1 cut(s) | 344 |
| FaiI | YATR | 9 cut(s) | 85, 87, 141, 316, 318, 322, 324, 342, 457 |
| FaqI | GGGAC | 1 cut(s) | 74 |
| FatI | CATG | 1 cut(s) | 340 |
| FauNDI | CATATG | 2 cut(s) | 85, 322 |
| FbaI | TGATCA | 1 cut(s) | 12 |
| FblI | GTMKAC | 1 cut(s) | 284 |
| Fnu4HI | GCNGC | 1 cut(s) | 114 |
| Fsp4HI | GCNGC | 1 cut(s) | 114 |
| FspBI | CTAG | 1 cut(s) | 302 |
| GlaI | GCGC | 1 cut(s) | 339 |
| GluI | GCNGC | 1 cut(s) | 114 |
| HaeIII | GGCC | 1 cut(s) | 391 |
| HapII | CCGG | 1 cut(s) | 173 |
| HhaI | GCGC | 1 cut(s) | 340 |
| Hin1I | GRCGYC | 1 cut(s) | 201 |
| Hin1II | CATG | 1 cut(s) | 344 |
| Hin6I | GCGC | 1 cut(s) | 338 |
| HinP1I | GCGC | 1 cut(s) | 338 |
| HindIII | AAGCTT | 1 cut(s) | 275 |
| HpaII | CCGG | 1 cut(s) | 173 |
| HphI | GGTGA | 2 cut(s) | 209, 286 |
| Hpy166II | GTNNAC | 1 cut(s) | 285 |
| Hpy188I | TCNGA | 1 cut(s) | 47 |
| Hpy188III | TCNNGA | 1 cut(s) | 310 |
| Hpy8I | GTNNAC | 1 cut(s) | 285 |
| HpyAV | CCTTC | 1 cut(s) | 50 |
| HpyCH4III | ACNGT | 1 cut(s) | 249 |
| HpyCH4IV | ACGT | 1 cut(s) | 201 |
| HpyCH4V | TGCA | 1 cut(s) | 433 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 274 |
| HpyF3I | CTNAG | 3 cut(s) | 44, 257, 349 |
| HpySE526I | ACGT | 1 cut(s) | 201 |
| Hsp92I | GRCGYC | 1 cut(s) | 201 |
| Hsp92II | CATG | 1 cut(s) | 344 |
| HspAI | GCGC | 1 cut(s) | 338 |
| Ksp22I | TGATCA | 1 cut(s) | 12 |
| Kzo9I | GATC | 4 cut(s) | 12, 142, 345, 407 |
| LguI | GCTCTTC | 1 cut(s) | 241 |
| Lsp1109I | GCAGC | 1 cut(s) | 100 |
| MaeI | CTAG | 1 cut(s) | 302 |
| MaeII | ACGT | 1 cut(s) | 201 |
| MaeIII | GTNAC | 1 cut(s) | 197 |
| MalI | GATC | 4 cut(s) | 14, 144, 347, 409 |
| MboI | GATC | 4 cut(s) | 12, 142, 345, 407 |
| MboII | GAAGA | 5 cut(s) | 76, 173, 188, 228, 434 |
| MflI | RGATCY | 1 cut(s) | 345 |
| MhlI | GDGCHC | 1 cut(s) | 39 |
| MluCI | AATT | 3 cut(s) | 215, 305, 443 |
| MnlI | CCTC | 5 cut(s) | 13, 54, 157, 175, 178 |
| MseI | TTAA | 3 cut(s) | 243, 251, 279 |
| MslI | CAYNNNNRTG | 1 cut(s) | 20 |
| MspA1I | CMGCKG | 1 cut(s) | 413 |
| MspCI | CTTAAG | 1 cut(s) | 278 |
| MspI | CCGG | 1 cut(s) | 173 |
| MspR9I | CCNGG | 2 cut(s) | 93, 173 |
| Mva1269I | GAATGC | 1 cut(s) | 290 |
| MvaI | CCWGG | 1 cut(s) | 93 |
| MvnI | CGCG | 1 cut(s) | 311 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 274 |
| NciI | CCSGG | 1 cut(s) | 173 |
| NdeI | CATATG | 2 cut(s) | 85, 322 |
| NdeII | GATC | 4 cut(s) | 12, 142, 345, 407 |
| NlaIII | CATG | 1 cut(s) | 344 |
| NlaIV | GGNNCC | 1 cut(s) | 453 |
| NmuCI | GTSAC | 1 cut(s) | 197 |
| NruI | TCGCGA | 1 cut(s) | 311 |
| NspV | TTCGAA | 1 cut(s) | 213 |
| OliI | CACNNNNGTG | 1 cut(s) | 20 |
| PasI | CCCWGGG | 1 cut(s) | 92 |
| PciSI | GCTCTTC | 1 cut(s) | 241 |
| PctI | GAATGC | 1 cut(s) | 290 |
| PfoI | TCCNGGA | 1 cut(s) | 171 |
| PkrI | GCNGC | 1 cut(s) | 115 |
| Psp6I | CCWGG | 1 cut(s) | 91 |
| PspGI | CCWGG | 1 cut(s) | 91 |
| PspN4I | GGNNCC | 1 cut(s) | 453 |
| PspPI | GGNCC | 1 cut(s) | 390 |
| PsuI | RGATCY | 1 cut(s) | 345 |
| PvuII | CAGCTG | 1 cut(s) | 413 |
| RruI | TCGCGA | 1 cut(s) | 311 |
| RseI | CAYNNNNRTG | 1 cut(s) | 20 |
| SapI | GCTCTTC | 1 cut(s) | 241 |
| SaqAI | TTAA | 3 cut(s) | 243, 251, 279 |
| SatI | GCNGC | 1 cut(s) | 114 |
| Sau3AI | GATC | 4 cut(s) | 12, 142, 345, 407 |
| Sau96I | GGNCC | 1 cut(s) | 390 |
| ScrFI | CCNGG | 2 cut(s) | 93, 173 |
| SduI | GDGCHC | 1 cut(s) | 39 |
| SfuI | TTCGAA | 1 cut(s) | 213 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 20 |
| SmlI | CTYRAG | 1 cut(s) | 278 |
| SmoI | CTYRAG | 1 cut(s) | 278 |
| Sse9I | AATT | 3 cut(s) | 215, 305, 443 |
| SspMI | CTAG | 1 cut(s) | 302 |
| StyD4I | CCNGG | 2 cut(s) | 91, 171 |
| TaaI | ACNGT | 1 cut(s) | 249 |
| TaiI | ACGT | 1 cut(s) | 204 |
| TaqI | TCGA | 1 cut(s) | 213 |
| TasI | AATT | 3 cut(s) | 215, 305, 443 |
| Tru1I | TTAA | 3 cut(s) | 243, 251, 279 |
| Tru9I | TTAA | 3 cut(s) | 243, 251, 279 |
| TseFI | GTSAC | 1 cut(s) | 197 |
| TseI | GCWGC | 1 cut(s) | 113 |
| Tsp45I | GTSAC | 1 cut(s) | 197 |
| Vha464I | CTTAAG | 1 cut(s) | 278 |
| XapI | RAATTY | 1 cut(s) | 305 |
| XmiI | GTMKAC | 1 cut(s) | 284 |
| XspI | CTAG | 1 cut(s) | 302 |
| ZraI | GACGTC | 1 cut(s) | 202 |