No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 169 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 670 |
| AccBSI | CCGCTC | 1 cut(s) | 284 |
| AccI | GTMKAC | 1 cut(s) | 500 |
| AccII | CGCG | 1 cut(s) | 35 |
| AciI | CCGC | 8 cut(s) | 12, 125, 284, 437, 566, 630, 642, 683 |
| AclWI | GGATC | 2 cut(s) | 323, 554 |
| AcoI | YGGCCR | 2 cut(s) | 355, 438 |
| AcsI | RAATTY | 1 cut(s) | 528 |
| AcuI | CTGAAG | 1 cut(s) | 632 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 316, 670, 689 |
| AgsI | TTSAA | 2 cut(s) | 535, 581 |
| AjnI | CCWGG | 2 cut(s) | 636, 688 |
| AleI | CACNNNNGTG | 1 cut(s) | 520 |
| AluBI | AGCT | 2 cut(s) | 82, 473 |
| AluI | AGCT | 2 cut(s) | 82, 473 |
| Alw26I | GTCTC | 1 cut(s) | 653 |
| AlwI | GGATC | 2 cut(s) | 323, 554 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 371 |
| Ama87I | CYCGRG | 1 cut(s) | 196 |
| AoxI | GGCC | 5 cut(s) | 189, 355, 438, 639, 691 |
| ApeKI | GCWGC | 3 cut(s) | 411, 434, 512 |
| ApoI | RAATTY | 1 cut(s) | 528 |
| AspLEI | GCGC | 1 cut(s) | 72 |
| AspS9I | GGNCC | 2 cut(s) | 307, 692 |
| AsuHPI | GGTGA | 1 cut(s) | 554 |
| AsuNHI | GCTAGC | 2 cut(s) | 407, 632 |
| AvaI | CYCGRG | 1 cut(s) | 196 |
| AvaII | GGWCC | 1 cut(s) | 307 |
| AxyI | CCTNAGG | 1 cut(s) | 364 |
| BalI | TGGCCA | 1 cut(s) | 357 |
| BbvCI | CCTCAGC | 1 cut(s) | 87 |
| BbvI | GCAGC | 3 cut(s) | 423, 446, 524 |
| BccI | CCATC | 2 cut(s) | 200, 347 |
| BceAI | ACGGC | 2 cut(s) | 412, 425 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 56, 90 |
| BciT130I | CCWGG | 2 cut(s) | 638, 690 |
| BciVI | GTATCC | 1 cut(s) | 85 |
| BcoDI | GTCTC | 1 cut(s) | 653 |
| BfaI | CTAG | 6 cut(s) | 62, 323, 396, 408, 476, 633 |
| BfmI | CTRYAG | 2 cut(s) | 432, 555 |
| BfuAI | ACCTGC | 1 cut(s) | 169 |
| BfuI | GTATCC | 1 cut(s) | 85 |
| BisI | GCNGC | 7 cut(s) | 12, 412, 435, 438, 513, 567, 642 |
| BlsI | GCNGC | 7 cut(s) | 13, 413, 436, 439, 514, 568, 643 |
| Bme1390I | CCNGG | 2 cut(s) | 638, 690 |
| Bme18I | GGWCC | 1 cut(s) | 307 |
| BmeT110I | CYCGRG | 1 cut(s) | 196 |
| BmgT120I | GGNCC | 2 cut(s) | 307, 692 |
| BmrFI | CCNGG | 2 cut(s) | 638, 690 |
| BmtI | GCTAGC | 2 cut(s) | 411, 636 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 179, 211 |
| BpmI | CTGGAG | 1 cut(s) | 635 |
| Bpu10I | CCTNAGC | 1 cut(s) | 87 |
| BsaAI | YACGTR | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 1 cut(s) | 343 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 316, 670, 689 |
| Bse21I | CCTNAGG | 1 cut(s) | 364 |
| Bse3DI | GCAATG | 1 cut(s) | 72 |
| BseBI | CCWGG | 2 cut(s) | 638, 690 |
| BseDI | CCNNGG | 1 cut(s) | 343 |
| BseGI | GGATG | 1 cut(s) | 339 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 316, 670, 689 |
| BseMI | GCAATG | 1 cut(s) | 72 |
| BseMII | CTCAG | 3 cut(s) | 78, 108, 378 |
| BseX3I | CGGCCG | 1 cut(s) | 438 |
| BseXI | GCAGC | 3 cut(s) | 423, 446, 524 |
| BseYI | CCCAGC | 1 cut(s) | 78 |
| Bsh1236I | CGCG | 1 cut(s) | 35 |
| Bsh1285I | CGRYCG | 2 cut(s) | 441, 630 |
| BshFI | GGCC | 5 cut(s) | 191, 357, 440, 641, 693 |
| BsiEI | CGRYCG | 2 cut(s) | 441, 630 |
| BsiHKCI | CYCGRG | 1 cut(s) | 196 |
| BslFI | GGGAC | 1 cut(s) | 479 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 316, 670, 689 |
| BsmAI | GTCTC | 1 cut(s) | 653 |
| BsmFI | GGGAC | 1 cut(s) | 479 |
| BsmI | GAATGC | 2 cut(s) | 127, 397 |
| BsnI | GGCC | 5 cut(s) | 191, 357, 440, 641, 693 |
| BsoBI | CYCGRG | 1 cut(s) | 196 |
| Bsp143I | GATC | 3 cut(s) | 43, 328, 559 |
| Bsp68I | TCGCGA | 1 cut(s) | 35 |
| BspACI | CCGC | 8 cut(s) | 12, 125, 284, 437, 566, 630, 642, 683 |
| BspANI | GGCC | 5 cut(s) | 191, 357, 440, 641, 693 |
| BspCNI | CTCAG | 3 cut(s) | 79, 109, 377 |
| BspFNI | CGCG | 1 cut(s) | 35 |
| BspMAI | CTGCAG | 1 cut(s) | 436 |
| BspMI | ACCTGC | 1 cut(s) | 169 |
| BspOI | GCTAGC | 2 cut(s) | 411, 636 |
| BspPI | GGATC | 2 cut(s) | 323, 554 |
| BsrBI | CCGCTC | 1 cut(s) | 284 |
| BsrDI | GCAATG | 1 cut(s) | 72 |
| BssECI | CCNNGG | 1 cut(s) | 343 |
| BssMI | GATC | 3 cut(s) | 43, 328, 559 |
| Bst2UI | CCWGG | 2 cut(s) | 638, 690 |
| Bst4CI | ACNGT | 1 cut(s) | 114 |
| Bst6I | CTCTTC | 1 cut(s) | 582 |
| BstBAI | YACGTR | 1 cut(s) | 56 |
| BstC8I | GCNNGC | 4 cut(s) | 84, 167, 409, 634 |
| BstDEI | CTNAG | 4 cut(s) | 87, 96, 117, 364 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 314 |
| BstF5I | GGATG | 1 cut(s) | 339 |
| BstFNI | CGCG | 1 cut(s) | 35 |
| BstHHI | GCGC | 1 cut(s) | 72 |
| BstKTI | GATC | 3 cut(s) | 46, 331, 562 |
| BstMAI | GTCTC | 1 cut(s) | 653 |
| BstMBI | GATC | 3 cut(s) | 43, 328, 559 |
| BstMCI | CGRYCG | 2 cut(s) | 441, 630 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 83, 166, 175, 443, 638 |
| BstNI | CCWGG | 2 cut(s) | 638, 690 |
| BstNSI | RCATGY | 1 cut(s) | 648 |
| BstSCI | CCNGG | 2 cut(s) | 636, 688 |
| BstSFI | CTRYAG | 2 cut(s) | 432, 555 |
| BstUI | CGCG | 1 cut(s) | 35 |
| BstV1I | GCAGC | 3 cut(s) | 423, 446, 524 |
| BstX2I | RGATCY | 2 cut(s) | 328, 559 |
| BstYI | RGATCY | 2 cut(s) | 328, 559 |
| BstZI | CGGCCG | 1 cut(s) | 438 |
| Bsu36I | CCTNAGG | 1 cut(s) | 364 |
| BsuI | GTATCC | 1 cut(s) | 85 |
| BsuRI | GGCC | 5 cut(s) | 191, 357, 440, 641, 693 |
| BtgZI | GCGATG | 1 cut(s) | 584 |
| BtsCI | GGATG | 1 cut(s) | 339 |
| BtsI | GCAGTG | 1 cut(s) | 417 |
| BtsIMutI | CAGTG | 1 cut(s) | 417 |
| BtuMI | TCGCGA | 1 cut(s) | 35 |
| BveI | ACCTGC | 1 cut(s) | 169 |
| Cac8I | GCNNGC | 4 cut(s) | 84, 167, 409, 634 |
| CaiI | CAGNNNCTG | 1 cut(s) | 371 |
| CfoI | GCGC | 1 cut(s) | 72 |
| Cfr13I | GGNCC | 2 cut(s) | 307, 692 |
| CseI | GACGC | 1 cut(s) | 207 |
| CviAII | CATG | 4 cut(s) | 137, 184, 274, 645 |
| DdeI | CTNAG | 4 cut(s) | 87, 96, 117, 364 |
| DpnI | GATC | 3 cut(s) | 45, 330, 561 |
| DpnII | GATC | 3 cut(s) | 43, 328, 559 |
| EaeI | YGGCCR | 2 cut(s) | 355, 438 |
| EagI | CGGCCG | 1 cut(s) | 438 |
| Eam1104I | CTCTTC | 1 cut(s) | 582 |
| EarI | CTCTTC | 1 cut(s) | 582 |
| EciI | GGCGGA | 1 cut(s) | 672 |
| EclXI | CGGCCG | 1 cut(s) | 438 |
| Eco47I | GGWCC | 1 cut(s) | 307 |
| Eco52I | CGGCCG | 1 cut(s) | 438 |
| Eco57I | CTGAAG | 1 cut(s) | 632 |
| Eco81I | CCTNAGG | 1 cut(s) | 364 |
| Eco88I | CYCGRG | 1 cut(s) | 196 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 314 |
| EcoRII | CCWGG | 2 cut(s) | 636, 688 |
| FaeI | CATG | 4 cut(s) | 140, 187, 277, 648 |
| FaiI | YATR | 9 cut(s) | 53, 138, 185, 213, 275, 383, 557, 646, 702 |
| FaqI | GGGAC | 1 cut(s) | 479 |
| FatI | CATG | 4 cut(s) | 136, 183, 273, 644 |
| FblI | GTMKAC | 1 cut(s) | 500 |
| Fnu4HI | GCNGC | 7 cut(s) | 12, 412, 435, 438, 513, 567, 642 |
| FokI | GGATG | 1 cut(s) | 326 |
| Fsp4HI | GCNGC | 7 cut(s) | 12, 412, 435, 438, 513, 567, 642 |
| FspBI | CTAG | 6 cut(s) | 62, 323, 396, 408, 476, 633 |
| GlaI | GCGC | 1 cut(s) | 71 |
| GluI | GCNGC | 7 cut(s) | 12, 412, 435, 438, 513, 567, 642 |
| GsaI | CCCAGC | 1 cut(s) | 82 |
| GsuI | CTGGAG | 1 cut(s) | 635 |
| HaeIII | GGCC | 5 cut(s) | 191, 357, 440, 641, 693 |
| HgaI | GACGC | 1 cut(s) | 207 |
| HhaI | GCGC | 1 cut(s) | 72 |
| Hin1II | CATG | 4 cut(s) | 140, 187, 277, 648 |
| Hin6I | GCGC | 1 cut(s) | 70 |
| HinP1I | GCGC | 1 cut(s) | 70 |
| HincII | GTYRAC | 1 cut(s) | 453 |
| HindII | GTYRAC | 1 cut(s) | 453 |
| HinfI | GANTC | 1 cut(s) | 574 |
| HphI | GGTGA | 1 cut(s) | 554 |
| Hpy166II | GTNNAC | 3 cut(s) | 59, 453, 501 |
| Hpy188I | TCNGA | 4 cut(s) | 118, 223, 390, 612 |
| Hpy188III | TCNNGA | 4 cut(s) | 34, 196, 310, 652 |
| Hpy8I | GTNNAC | 3 cut(s) | 59, 453, 501 |
| Hpy99I | CGWCG | 1 cut(s) | 542 |
| HpyAV | CCTTC | 4 cut(s) | 239, 539, 607, 611 |
| HpyCH4III | ACNGT | 1 cut(s) | 114 |
| HpyCH4IV | ACGT | 3 cut(s) | 55, 537, 669 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 277, 434 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 83, 166, 175, 443, 638 |
| HpyF3I | CTNAG | 4 cut(s) | 87, 96, 117, 364 |
| HpySE526I | ACGT | 3 cut(s) | 55, 537, 669 |
| Hsp92II | CATG | 4 cut(s) | 140, 187, 277, 648 |
| HspAI | GCGC | 1 cut(s) | 70 |
| Kzo9I | GATC | 3 cut(s) | 43, 328, 559 |
| Lsp1109I | GCAGC | 3 cut(s) | 423, 446, 524 |
| MaeI | CTAG | 6 cut(s) | 62, 323, 396, 408, 476, 633 |
| MaeII | ACGT | 3 cut(s) | 55, 537, 669 |
| MalI | GATC | 3 cut(s) | 45, 330, 561 |
| MbiI | CCGCTC | 1 cut(s) | 284 |
| MboI | GATC | 3 cut(s) | 43, 328, 559 |
| MboII | GAAGA | 1 cut(s) | 569 |
| MflI | RGATCY | 2 cut(s) | 328, 559 |
| MlsI | TGGCCA | 1 cut(s) | 357 |
| MluCI | AATT | 3 cut(s) | 105, 287, 528 |
| MluNI | TGGCCA | 1 cut(s) | 357 |
| MlyI | GAGTC | 1 cut(s) | 568 |
| Mox20I | TGGCCA | 1 cut(s) | 357 |
| MscI | TGGCCA | 1 cut(s) | 357 |
| MslI | CAYNNNNRTG | 1 cut(s) | 520 |
| Msp20I | TGGCCA | 1 cut(s) | 357 |
| MspA1I | CMGCKG | 2 cut(s) | 14, 437 |
| MspR9I | CCNGG | 2 cut(s) | 638, 690 |
| Mva1269I | GAATGC | 2 cut(s) | 127, 397 |
| MvaI | CCWGG | 2 cut(s) | 638, 690 |
| MvnI | CGCG | 1 cut(s) | 35 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 83, 166, 175, 443, 638 |
| NdeII | GATC | 3 cut(s) | 43, 328, 559 |
| NheI | GCTAGC | 2 cut(s) | 407, 632 |
| NlaIII | CATG | 4 cut(s) | 140, 187, 277, 648 |
| NruI | TCGCGA | 1 cut(s) | 35 |
| NspI | RCATGY | 1 cut(s) | 648 |
| OliI | CACNNNNGTG | 1 cut(s) | 520 |
| PaeR7I | CTCGAG | 1 cut(s) | 196 |
| PctI | GAATGC | 2 cut(s) | 127, 397 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 670 |
| PkrI | GCNGC | 7 cut(s) | 13, 413, 436, 439, 514, 568, 643 |
| PleI | GAGTC | 1 cut(s) | 568 |
| PpsI | GAGTC | 1 cut(s) | 568 |
| Ppu21I | YACGTR | 1 cut(s) | 56 |
| Psp6I | CCWGG | 2 cut(s) | 636, 688 |
| PspFI | CCCAGC | 1 cut(s) | 78 |
| PspGI | CCWGG | 2 cut(s) | 636, 688 |
| PspPI | GGNCC | 2 cut(s) | 307, 692 |
| PstI | CTGCAG | 1 cut(s) | 436 |
| PstNI | CAGNNNCTG | 1 cut(s) | 371 |
| PsuI | RGATCY | 2 cut(s) | 328, 559 |
| RruI | TCGCGA | 1 cut(s) | 35 |
| RseI | CAYNNNNRTG | 1 cut(s) | 520 |
| SatI | GCNGC | 7 cut(s) | 12, 412, 435, 438, 513, 567, 642 |
| Sau3AI | GATC | 3 cut(s) | 43, 328, 559 |
| Sau96I | GGNCC | 2 cut(s) | 307, 692 |
| SchI | GAGTC | 1 cut(s) | 568 |
| ScrFI | CCNGG | 2 cut(s) | 638, 690 |
| SfcI | CTRYAG | 2 cut(s) | 432, 555 |
| Sfr274I | CTCGAG | 1 cut(s) | 196 |
| SinI | GGWCC | 1 cut(s) | 307 |
| SlaI | CTCGAG | 1 cut(s) | 196 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 520 |
| SmlI | CTYRAG | 1 cut(s) | 196 |
| SmoI | CTYRAG | 1 cut(s) | 196 |
| Sse9I | AATT | 3 cut(s) | 105, 287, 528 |
| SsiI | CCGC | 8 cut(s) | 12, 125, 284, 437, 566, 630, 642, 683 |
| SspMI | CTAG | 6 cut(s) | 62, 323, 396, 408, 476, 633 |
| StyD4I | CCNGG | 2 cut(s) | 636, 688 |
| TaaI | ACNGT | 1 cut(s) | 114 |
| TaiI | ACGT | 3 cut(s) | 58, 540, 672 |
| TaqI | TCGA | 1 cut(s) | 197 |
| TasI | AATT | 3 cut(s) | 105, 287, 528 |
| TauI | GCSGC | 4 cut(s) | 14, 440, 569, 644 |
| TscAI | CASTG | 1 cut(s) | 424 |
| TseI | GCWGC | 3 cut(s) | 411, 434, 512 |
| TspDTI | ATGAA | 1 cut(s) | 125 |
| TspRI | CASTG | 1 cut(s) | 424 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 670 |
| VpaK11BI | GGWCC | 1 cut(s) | 307 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 314 |
| XapI | RAATTY | 1 cut(s) | 528 |
| XceI | RCATGY | 1 cut(s) | 648 |
| XhoI | CTCGAG | 1 cut(s) | 196 |
| XmiI | GTMKAC | 1 cut(s) | 500 |
| XspI | CTAG | 6 cut(s) | 62, 323, 396, 408, 476, 633 |