FvH4_2g18071

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000071
Physical Location & Seq
Reverse (-)
15476794 .. 15479610
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18071.t1

Sequence Viewer

Length: 2817 bp
ATGCCTAAGTACCTTTGGGGAGAAGCCGTGTTGTGTGCGTCTCACCTAATCAATCGTCTCCCGTCAGCTCCTCTTCAAGGTCGTGTTCCCTTTGATGTCTTGTCTCAATACGTTCCTATCCCATCATCAAACACTCTTCCTGCTCGTGTTTTTGGTTGTGTTGCTTATGTCCATTTGTATAATAATCAAAGGTCAAAATTGGATGCTAGAGCCCTTAAGTGTGTGTTTGTAGGGTATGGTTCCCATCAGAAAGGTTATAAGTGTTATCATCCTCCATCCCAAAGAATATATGTCACCATGGATGTTACATTTAGTGAAGATACATGTTATTTTTTGCCTCCTGTAACTCCTCTCCAGGGGGAGAAGTCATATTTTTATGAAGATTTGTTTAGCGAGTCAACCAGGAGTCAAGGTTTTGAGGAGTCACCAAAGGACAATAAAGGGAATGAGTCAGTTTTGCAGAAACATAAAAGAGGCAGTGAACATGAGCGACTGTCGTTATCTGTTGAGTTCAGACCCGATACCGGTGTTCCGCCAGAGCTCCCAGGCTCGCCGACGCTGTCTCCGTCCGATCGCCCAGGCTCGCCGGCCCGTTCTCCGTCCGAGAGCCCAGGCTCGCCGGCGCTGTCTCCGTCTGAGTGTCTAGCCTCACCGGCCCGTTCTCCGTCCGAGGGCCCAGCCAATAGTGGTTCTCCAACCGGATTTCAGCATATTTCCGATCAACTGACCGGAGCAAAAAGCAGAGAGTTAGGGTTCCCAGATCAGACTAGCTCCACCTGTCCATTAATCGGAGACAATCGATCTCATCTTCCTGTCTCGTCGGTTTCCGGGGCCCTGACTCCGCCGGTCGTAGGCGACCTTCTAGAAAGCAACGTGACCGGTTGTCATCCAGTTTTGGGCAGTGACCCTGTCAGTTCTGCATCAACAAATCAATCAGGGTCAGATTCTAGTCAAGGCCATGATTCGTCAGTGAGGAAGAAGATTTCTGATCAAGGCCATGCTTCGTCAGTCAGGGAGCAAAATTCGAATGTGAACAGTGCTCCTGCCCCTCCCTCACCTTTACAGGTCTCTCCGGCTCATGCATCACCCGAGGTACGTTCTACTCATTCTTCCGTGTCTGTTAAACAATTACCTAACCGTGTCACTCGTGGTCAACCTCCAAAACACTATGAACCTACCCTTAGTGCAAAAACTCGGTATCCTGTTGCTAATTATGTGTCAACCCATAGATTGTCTAAACCATATGCTGCGTTTGTGAATCAATTATCTACTGTATCTCTCCCTAGTAAAGTGCAGGATGCTATGCGTGATGTTAAGTGGATGCAAGCAATGAAAGTTGAGATGGAAGCTCTTGAGAAGAATTGTACGTGGGAGTTGGTACCATTACCACGGGGTAAGAAGACAGTTGGTTGTCGGTGGGTGTATAGTGTGAAACATAATCCTGATGGTTCAGTGGATAGGTACAAGGCAAGGTTAGTCGCTAAAGGCTACACTCAGAAGTATGGGGTGGATTATGACGAGACGTTTGCACCAGTGGCAAAAATTAATACAATACGAGTACTTCTTTCGTTAGCCGTTAATCTTGATTGGCCTTTGCAGCAATTTGATGTTAAGAATGCTTTCTTGCATGGTGATTTGCATGAAGAAGTCTATATGGATTTACCTCCTGGATATGGTACTTCTACTGGAGAGCAGGTAGTGTGCAAGTTGAAGAAGTCCCTTTACGGTCTAAAGCAATCACCCAGAGCATGGTTTGGACGTTTTTCTAAGTTCATGAAGAGGATTGGATACCGACAGAGTAACTCAGATCACACTTTGTTTCTTAAACACCGGTGTGGTAAGGTGACAGCTTTAATTATTTATGTAGATGATATGGTTGTGACAGGTGATGACAATGAGGAAATTCAGAGATTACAAGATTTGTTGTCTTCTGAATTTGAGATGAAAGATTTGGGCAGTTTGAAGTATTTCTTGGGAATTGAAGTTGCTCGTGGGAAGGATTGTATTATGCTAAGTCAGAGAAAATATGTCCTAGATTTGTTAGCAGAAACGGGCATGCTTGACTGTAAACCAGCTGATACCCCTATTGAACAAAATCATCAGTTAGCAGAGTATTTGGATCAAGTGCCTACAAATAAGGCACGATATCAGAGGTTAGTTGGCCGATTGATTTATTTGTCTCACACTAGACCAGATTTAGCCTATGCAGTTAGTGTTGTGAGTCAGTTTATGCATAATCCTAGTGAAGCTCATATGGAAGCTGTATTTCGTATCTTGAAGTATTTGAAGTCTGCTCCAGGGAAGGGGTTAATATTTTCAAAACACAAGCACTTGGATGTTTCTGGGTACACAGACTCAGACTGGGCAGGTAATATTATAGATCGCAGGTCCACTTCCGGCTACTTCACTTTCGTTGGTGGTAATTTGGTTACTTGGAAAAGTAAGAAACAAAAGGTGGTAGCTCGCTCCAGTGCTGAAGCAGAGTATCGTGGAATGGCTCTTGGAATATGTGAGATGTTGTGGTTGAGACATTTGTTGAGAGATTTGGGATTCAGACAAAAAAAGGCAATGCCTCTATATTGTGATAATAAAGCTGCGGTTGAAATTGCTCATAATCCAGTTCAGCATGACAGAACAAAACATGTGGAGGTCGATCAACATTTCATTAAGGAGAAGTTGGATCGACAGATTATTGTGTTCCCATTTGTACCTACCGAGGAGCAGCTGGCAGACATTCTTACGAAAGCAGTCTCTAGTAAGGCGTTTTATGACTCACTTGACAAGTTGGGCATCTGTGATCTGTATGCTCCAACTTGA

Protein Analysis

939

Amino Acids

104.26

Weight (kDa)

8.78

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 53 - 110 8.9e-23 Retroviral polymerase SH3-like domain
RVT_2 PF07727 455 - 697 1.4e-72 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
Acc36I ACCTGC 3 cut(s) 1684, 2357, 2376
Acc65I GGTACC 1 cut(s) 1378
AccB1I GGYRCC 1 cut(s) 1378
AccB7I CCANNNNNTGG 1 cut(s) 1749
AciI CCGC 3 cut(s) 533, 842, 2597
AclWI GGATC 2 cut(s) 2127, 2688
AcoI YGGCCR 1 cut(s) 2161
AcsI RAATTY 3 cut(s) 1021, 1902, 1934
AcuI CTGAAG 1 cut(s) 2496
AfaI GTAC 9 cut(s) 11, 1095, 1366, 1380, 1463, 1560, 1678, 2348, 2709
AflII CTTAAG 1 cut(s) 215
AflIII ACRYGT 2 cut(s) 323, 2641
AgeI ACCGGT 3 cut(s) 524, 878, 1830
AgsI TTSAA 9 cut(s) 77, 1711, 1963, 1982, 2090, 2278, 2287, 2319, 2603
AhdI GACNNNNNGTC 1 cut(s) 882
AjnI CCWGG 7 cut(s) 354, 401, 544, 577, 610, 1666, 2296
AjuI GAANNNNNNNTTGG 2 cut(s) 1955, 1987
AleI CACNNNNGTG 1 cut(s) 1833
Alw21I GWGCWC 2 cut(s) 543, 1042
AlwI GGATC 2 cut(s) 2127, 2688
Ama87I CYCGRG 1 cut(s) 1088
AoxI GGCC 8 cut(s) 588, 654, 673, 831, 955, 994, 1589, 2161
ApaI GGGCCC 2 cut(s) 677, 835
ApeKI GCWGC 4 cut(s) 1247, 1597, 2594, 2722
ApoI RAATTY 3 cut(s) 1021, 1902, 1934
ArsI GACNNNNNNTTYG 2 cut(s) 1508, 1540
AseI ATTAAT 2 cut(s) 785, 1545
AsiGI ACCGGT 3 cut(s) 524, 878, 1830
Asp700I GAANNNNTTC 2 cut(s) 1619, 1967
Asp718I GGTACC 1 cut(s) 1378
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 7 cut(s) 589, 655, 673, 674, 831, 832, 2388
AsuC2I CCSGG 1 cut(s) 829
AsuII TTCGAA 1 cut(s) 1025
AvaI CYCGRG 1 cut(s) 1088
AvaII GGWCC 1 cut(s) 2388
BaeGI GKGCMC 2 cut(s) 677, 835
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 1 cut(s) 1378
BanII GRGCYC 5 cut(s) 214, 543, 611, 677, 835
BarI GAAGNNNNNNTAC 4 cut(s) 843, 875, 1706, 1738
BauI CACGAG 3 cut(s) 144, 1146, 1989
BbsI GAAGAC 2 cut(s) 1406, 1920
Bbv12I GWGCWC 2 cut(s) 543, 1042
BbvI GCAGC 4 cut(s) 1234, 1609, 2581, 2734
BccI CCATC 5 cut(s) 130, 252, 283, 1336, 1439
BceAI ACGGC 2 cut(s) 11, 1559
BcgI CGANNNNNNTGC 2 cut(s) 1508, 1542
BciT130I CCWGG 7 cut(s) 356, 403, 546, 579, 612, 1668, 2298
BciVI GTATCC 2 cut(s) 1209, 1781
BclI TGATCA 1 cut(s) 988
BcnI CCSGG 1 cut(s) 829
BfoI RGCGCY 1 cut(s) 626
BfrI CTTAAG 1 cut(s) 215
BfuAI ACCTGC 3 cut(s) 1684, 2357, 2376
BfuI GTATCC 2 cut(s) 1209, 1781
BglI GCCNNNNNGGC 1 cut(s) 653
BisI GCNGC 4 cut(s) 1248, 1598, 2595, 2723
BlsI GCNGC 4 cut(s) 1249, 1599, 2596, 2724
BmcAI AGTACT 1 cut(s) 1560
Bme1390I CCNGG 8 cut(s) 356, 403, 546, 579, 612, 829, 1668, 2298
Bme18I GGWCC 1 cut(s) 2388
BmeRI GACNNNNNGTC 1 cut(s) 882
BmeT110I CYCGRG 1 cut(s) 1088
BmgT120I GGNCC 7 cut(s) 589, 655, 673, 674, 831, 832, 2388
BmiI GGNNCC 6 cut(s) 241, 675, 755, 832, 833, 1380
BmrFI CCNGG 8 cut(s) 356, 403, 546, 579, 612, 829, 1668, 2298
BmrI ACTGGG 1 cut(s) 2371
BmsI GCATC 6 cut(s) 193, 929, 1091, 1288, 1311, 2799
BmuI ACTGGG 1 cut(s) 2371
BpiI GAAGAC 2 cut(s) 1406, 1920
BpmI CTGGAG 4 cut(s) 338, 1707, 2280, 2452
Bpu14I TTCGAA 1 cut(s) 1025
BpuEI CTTGAG 1 cut(s) 1373
BpuMI CCSGG 1 cut(s) 829
Bsa29I ATCGAT 1 cut(s) 799
BsaAI YACGTR 1 cut(s) 1368
BsaI GGTCTC 1 cut(s) 1072
BsaWI WCCGGW 5 cut(s) 524, 698, 728, 878, 1830
BsaXI ACNNNNNCTCC 8 cut(s) 12, 42, 397, 427, 547, 577, 1680, 1710
Bse118I RCCGGY 7 cut(s) 524, 586, 619, 652, 844, 878, 1830
Bse1I ACTGG 6 cut(s) 890, 1532, 1690, 2366, 2469, 2618
Bse3DI GCAATG 2 cut(s) 1335, 2574
BseBI CCWGG 7 cut(s) 356, 403, 546, 579, 612, 1668, 2298
BseCI ATCGAT 1 cut(s) 799
BseGI GGATG 8 cut(s) 208, 268, 275, 307, 886, 1303, 1326, 2341
BseMI GCAATG 2 cut(s) 1335, 2574
BseMII CTCAG 4 cut(s) 627, 1508, 1818, 2370
BseNI ACTGG 6 cut(s) 890, 1532, 1690, 2366, 2469, 2618
BseRI GAGGAG 4 cut(s) 60, 339, 434, 2732
BseSI GKGCMC 2 cut(s) 677, 835
BseXI GCAGC 4 cut(s) 1234, 1609, 2581, 2734
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 1 cut(s) 1313
Bsh1285I CGRYCG 2 cut(s) 574, 849
BshFI GGCC 8 cut(s) 590, 656, 675, 833, 957, 996, 1591, 2163
BshNI GGYRCC 1 cut(s) 1378
BshTI ACCGGT 3 cut(s) 524, 878, 1830
BshVI ATCGAT 1 cut(s) 799
BsiEI CGRYCG 2 cut(s) 574, 849
BsiHKAI GWGCWC 2 cut(s) 543, 1042
BsiHKCI CYCGRG 1 cut(s) 1088
BslFI GGGAC 1 cut(s) 1702
BsmBI CGTCTC 3 cut(s) 45, 62, 1514
BsmFI GGGAC 1 cut(s) 1702
BsmI GAATGC 1 cut(s) 1621
BsnI GGCC 8 cut(s) 590, 656, 675, 833, 957, 996, 1591, 2163
Bso31I GGTCTC 1 cut(s) 1072
BsoBI CYCGRG 1 cut(s) 1088
Bsp119I TTCGAA 1 cut(s) 1025
Bsp120I GGGCCC 2 cut(s) 673, 831
Bsp1286I GDGCHC 6 cut(s) 214, 543, 611, 677, 835, 1042
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 3 cut(s) 533, 842, 2597
BspANI GGCC 8 cut(s) 590, 656, 675, 833, 957, 996, 1591, 2163
BspCNI CTCAG 4 cut(s) 628, 1507, 1817, 2369
BspDI ATCGAT 1 cut(s) 799
BspHI TCATGA 1 cut(s) 1773
BspLI GGNNCC 6 cut(s) 241, 675, 755, 832, 833, 1380
BspMI ACCTGC 3 cut(s) 1684, 2357, 2376
BspPI GGATC 2 cut(s) 2127, 2688
BspT104I TTCGAA 1 cut(s) 1025
BspT107I GGYRCC 1 cut(s) 1378
BspTI CTTAAG 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 1072
BsrDI GCAATG 2 cut(s) 1335, 2574
BsrFI RCCGGY 7 cut(s) 524, 586, 619, 652, 844, 878, 1830
BsrI ACTGG 6 cut(s) 890, 1532, 1690, 2366, 2469, 2618
BssAI RCCGGY 7 cut(s) 524, 586, 619, 652, 844, 878, 1830
BssSI CACGAG 3 cut(s) 144, 1146, 1989
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 3 cut(s) 144, 1146, 1989
Bst2UI CCWGG 7 cut(s) 356, 403, 546, 579, 612, 1668, 2298
Bst4CI ACNGT 7 cut(s) 495, 1037, 1139, 1273, 1405, 1727, 2066
Bst6I CTCTTC 3 cut(s) 78, 141, 1772
BstAFI CTTAAG 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 1368
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 9 cut(s) 551, 584, 588, 617, 621, 1326, 2057, 2464, 2727
BstDEI CTNAG 8 cut(s) 6, 636, 1181, 1494, 1767, 1804, 2012, 2356
BstDSI CCRYGG 2 cut(s) 297, 1388
BstENI CCTNNNNNAGG 1 cut(s) 75
BstF5I GGATG 8 cut(s) 208, 268, 275, 307, 886, 1303, 1326, 2341
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 1 cut(s) 625
BstMCI CGRYCG 2 cut(s) 574, 849
BstMWI GCNNNNNNNGC 3 cut(s) 653, 1535, 1597
BstNI CCWGG 7 cut(s) 356, 403, 546, 579, 612, 1668, 2298
BstNSI RCATGY 3 cut(s) 327, 2059, 2645
BstSCI CCNGG 8 cut(s) 354, 401, 544, 577, 610, 827, 1666, 2296
BstSLI GKGCMC 2 cut(s) 677, 835
BstV1I GCAGC 4 cut(s) 1234, 1609, 2581, 2734
BstV2I GAAGAC 2 cut(s) 1406, 1920
Bsu15I ATCGAT 1 cut(s) 799
BsuI GTATCC 2 cut(s) 1209, 1781
BsuRI GGCC 8 cut(s) 590, 656, 675, 833, 957, 996, 1591, 2163
BsuTUI ATCGAT 1 cut(s) 799
BtgI CCRYGG 2 cut(s) 297, 1388
BtsCI GGATG 8 cut(s) 208, 268, 275, 307, 886, 1303, 1326, 2341
BtsI GCAGTG 2 cut(s) 484, 907
BtsIMutI CAGTG 7 cut(s) 484, 907, 975, 1042, 1458, 1539, 2476
BveI ACCTGC 3 cut(s) 1684, 2357, 2376
Cac8I GCNNGC 9 cut(s) 551, 584, 588, 617, 621, 1326, 2057, 2464, 2727
CciI TCATGA 1 cut(s) 1773
CfoI GCGC 1 cut(s) 625
Cfr10I RCCGGY 7 cut(s) 524, 586, 619, 652, 844, 878, 1830
Cfr13I GGNCC 7 cut(s) 589, 655, 673, 674, 831, 832, 2388
ClaI ATCGAT 1 cut(s) 799
CseI GACGC 2 cut(s) 27, 565
Csp6I GTAC 9 cut(s) 10, 1094, 1365, 1379, 1462, 1559, 1677, 2347, 2708
CspAI ACCGGT 3 cut(s) 524, 878, 1830
CspCI CAANNNNNGTGG 2 cut(s) 2625, 2660
CviQI GTAC 9 cut(s) 10, 1094, 1365, 1379, 1462, 1559, 1677, 2347, 2708
DdeI CTNAG 8 cut(s) 6, 636, 1181, 1494, 1767, 1804, 2012, 2356
DriI GACNNNNNGTC 1 cut(s) 882
EaeI YGGCCR 1 cut(s) 2161
Eam1104I CTCTTC 3 cut(s) 78, 141, 1772
Eam1105I GACNNNNNGTC 1 cut(s) 882
EarI CTCTTC 3 cut(s) 78, 141, 1772
EciI GGCGGA 2 cut(s) 522, 831
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 5 cut(s) 214, 543, 611, 677, 835
Eco31I GGTCTC 1 cut(s) 1072
Eco32I GATATC 1 cut(s) 2147
Eco47I GGWCC 1 cut(s) 2388
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 2496
Eco88I CYCGRG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 75
EcoO109I RGGNCCY 3 cut(s) 673, 831, 832
EcoRII CCWGG 7 cut(s) 354, 401, 544, 577, 610, 1666, 2296
EcoRV GATATC 1 cut(s) 2147
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 2 cut(s) 1084, 2235
EcoT38I GRGCYC 5 cut(s) 214, 543, 611, 677, 835
ErhI CCWWGG 1 cut(s) 297
Esp3I CGTCTC 3 cut(s) 45, 62, 1514
FalI AAGNNNNNCTT 2 cut(s) 1955, 1987
FaqI GGGAC 1 cut(s) 1702
FauNDI CATATG 2 cut(s) 1243, 2253
FbaI TGATCA 1 cut(s) 988
Fnu4HI GCNGC 4 cut(s) 1248, 1598, 2595, 2723
FokI GGATG 8 cut(s) 215, 255, 262, 314, 873, 1310, 1333, 2348
FriOI GRGCYC 5 cut(s) 214, 543, 611, 677, 835
Fsp4HI GCNGC 4 cut(s) 1248, 1598, 2595, 2723
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 1248, 1598, 2595, 2723
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 4 cut(s) 338, 1707, 2280, 2452
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 8 cut(s) 590, 656, 675, 833, 957, 996, 1591, 2163
HgaI GACGC 2 cut(s) 27, 565
HhaI GCGC 1 cut(s) 625
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 3 cut(s) 399, 1154, 1221
HindII GTYRAC 3 cut(s) 399, 1154, 1221
Hpy166II GTNNAC 8 cut(s) 399, 482, 1033, 1154, 1221, 2069, 2349, 2391
Hpy188III TCNNGA 6 cut(s) 863, 1352, 1442, 1583, 1774, 2275
Hpy8I GTNNAC 8 cut(s) 399, 482, 1033, 1154, 1221, 2069, 2349, 2391
Hpy99I CGWCG 2 cut(s) 559, 823
HpyAV CCTTC 3 cut(s) 869, 1990, 2296
HpyCH4III ACNGT 7 cut(s) 495, 1037, 1139, 1273, 1405, 1727, 2066
HpyCH4IV ACGT 6 cut(s) 111, 873, 1096, 1367, 1523, 1759
HpyF10VI GCNNNNNNNGC 3 cut(s) 653, 1535, 1597
HpyF3I CTNAG 8 cut(s) 6, 636, 1181, 1494, 1767, 1804, 2012, 2356
HpySE526I ACGT 6 cut(s) 111, 873, 1096, 1367, 1523, 1759
HspAI GCGC 1 cut(s) 623
KpnI GGTACC 1 cut(s) 1382
KroI GCCGGC 2 cut(s) 586, 619
KroNI GCCGGC 2 cut(s) 588, 621
Ksp22I TGATCA 1 cut(s) 988
Lsp1109I GCAGC 4 cut(s) 1234, 1609, 2581, 2734
LweI GCATC 6 cut(s) 193, 929, 1091, 1288, 1311, 2799
MaeII ACGT 6 cut(s) 111, 873, 1096, 1367, 1523, 1759
MhlI GDGCHC 6 cut(s) 214, 543, 611, 677, 835, 1042
MlyI GAGTC 8 cut(s) 404, 415, 431, 458, 832, 2230, 2348, 2765
MmeI TCCRAC 2 cut(s) 719, 2658
Mph1103I ATGCAT 2 cut(s) 1084, 2235
MreI CGCCGGCG 1 cut(s) 619
MroNI GCCGGC 2 cut(s) 586, 619
MroXI GAANNNNTTC 2 cut(s) 1619, 1967
MslI CAYNNNNRTG 2 cut(s) 1833, 2334
MspA1I CMGCKG 2 cut(s) 2075, 2725
MspCI CTTAAG 1 cut(s) 215
MspR9I CCNGG 8 cut(s) 356, 403, 546, 579, 612, 829, 1668, 2298
Mva1269I GAATGC 1 cut(s) 1621
MvaI CCWGG 7 cut(s) 356, 403, 546, 579, 612, 1668, 2298
MwoI GCNNNNNNNGC 3 cut(s) 653, 1535, 1597
NaeI GCCGGC 2 cut(s) 588, 621
NciI CCSGG 1 cut(s) 829
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 2 cut(s) 1243, 2253
NgoMIV GCCGGC 2 cut(s) 586, 619
NlaIV GGNNCC 6 cut(s) 241, 675, 755, 832, 833, 1380
NmuCI GTSAC 7 cut(s) 292, 423, 874, 902, 1141, 1843, 1879
NsiI ATGCAT 2 cut(s) 1084, 2235
NspI RCATGY 3 cut(s) 327, 2059, 2645
NspV TTCGAA 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 1833
PaeI GCATGC 1 cut(s) 2059
PagI TCATGA 1 cut(s) 1773
PciI ACATGT 2 cut(s) 323, 2641
PcsI WCGNNNNNNNCGW 1 cut(s) 563
PctI GAATGC 1 cut(s) 1621
PdiI GCCGGC 2 cut(s) 588, 621
PdmI GAANNNNTTC 2 cut(s) 1619, 1967
PfeI GAWTC 4 cut(s) 944, 962, 1258, 2550
PflFI GACNNNGTC 2 cut(s) 559, 908
PflMI CCANNNNNTGG 1 cut(s) 1749
PfoI TCCNGGA 1 cut(s) 1666
PinAI ACCGGT 3 cut(s) 524, 878, 1830
PkrI GCNGC 4 cut(s) 1249, 1599, 2596, 2724
Ple19I CGATCG 1 cut(s) 574
PleI GAGTC 8 cut(s) 403, 414, 430, 457, 832, 2229, 2348, 2765
PpsI GAGTC 8 cut(s) 403, 414, 430, 457, 832, 2229, 2348, 2765
Ppu21I YACGTR 1 cut(s) 1368
PscI ACATGT 2 cut(s) 323, 2641
PshBI ATTAAT 2 cut(s) 785, 1545
PsiI TTATAA 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 7 cut(s) 354, 401, 544, 577, 610, 1666, 2296
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 7 cut(s) 354, 401, 544, 577, 610, 1666, 2296
PspN4I GGNNCC 6 cut(s) 241, 675, 755, 832, 833, 1380
PspOMI GGGCCC 2 cut(s) 673, 831
PspPI GGNCC 7 cut(s) 589, 655, 673, 674, 831, 832, 2388
PsyI GACNNNGTC 2 cut(s) 559, 908
PvuI CGATCG 1 cut(s) 574
PvuII CAGCTG 2 cut(s) 2075, 2725
RsaI GTAC 9 cut(s) 11, 1095, 1366, 1380, 1463, 1560, 1678, 2348, 2709
RsaNI GTAC 9 cut(s) 10, 1094, 1365, 1379, 1462, 1559, 1677, 2347, 2708
RseI CAYNNNNRTG 2 cut(s) 1833, 2334
SacI GAGCTC 1 cut(s) 543
SatI GCNGC 4 cut(s) 1248, 1598, 2595, 2723
Sau96I GGNCC 7 cut(s) 589, 655, 673, 674, 831, 832, 2388
ScaI AGTACT 1 cut(s) 1560
SchI GAGTC 8 cut(s) 404, 415, 431, 458, 832, 2230, 2348, 2765
ScrFI CCNGG 8 cut(s) 356, 403, 546, 579, 612, 829, 1668, 2298
SduI GDGCHC 6 cut(s) 214, 543, 611, 677, 835, 1042
SfaNI GCATC 6 cut(s) 193, 929, 1091, 1288, 1311, 2799
SfuI TTCGAA 1 cut(s) 1025
SgrAI CRCCGGYG 2 cut(s) 619, 1830
SinI GGWCC 1 cut(s) 2388
SmiMI CAYNNNNRTG 2 cut(s) 1833, 2334
SmlI CTYRAG 2 cut(s) 215, 1352
SmoI CTYRAG 2 cut(s) 215, 1352
SphI GCATGC 1 cut(s) 2059
SsiI CCGC 3 cut(s) 533, 842, 2597
SspI AATATT 2 cut(s) 2313, 2374
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 8 cut(s) 354, 401, 544, 577, 610, 827, 1666, 2296
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 7 cut(s) 495, 1037, 1139, 1273, 1405, 1727, 2066
TaiI ACGT 6 cut(s) 114, 876, 1099, 1370, 1526, 1762
TaqI TCGA 4 cut(s) 799, 1025, 2652, 2683
TatI WGTACW 1 cut(s) 1558
TfiI GAWTC 4 cut(s) 944, 962, 1258, 2550
TscAI CASTG 7 cut(s) 484, 907, 975, 1042, 1458, 1539, 2476
TseFI GTSAC 7 cut(s) 292, 423, 874, 902, 1141, 1843, 1879
TseI GCWGC 4 cut(s) 1247, 1597, 2594, 2722
Tsp45I GTSAC 7 cut(s) 292, 423, 874, 902, 1141, 1843, 1879
TspDTI ATGAA 8 cut(s) 393, 1185, 1346, 1656, 1762, 1790, 1958, 2653
TspGWI ACGGA 5 cut(s) 555, 588, 621, 654, 1102
TspRI CASTG 7 cut(s) 484, 907, 975, 1042, 1458, 1539, 2476
Tth111I GACNNNGTC 2 cut(s) 559, 908
Van91I CCANNNNNTGG 1 cut(s) 1749
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 2388
VspI ATTAAT 2 cut(s) 785, 1545
XagI CCTNNNNNAGG 1 cut(s) 75
XapI RAATTY 3 cut(s) 1021, 1902, 1934
XbaI TCTAGA 1 cut(s) 862
XceI RCATGY 3 cut(s) 327, 2059, 2645
XmnI GAANNNNTTC 2 cut(s) 1619, 1967
ZrmI AGTACT 1 cut(s) 1560
Zsp2I ATGCAT 2 cut(s) 1084, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.