Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 364 |
| AccB1I | GGYRCC | 1 cut(s) | 364 |
| AciI | CCGC | 1 cut(s) | 122 |
| AclWI | GGATC | 1 cut(s) | 251 |
| AcoI | YGGCCR | 1 cut(s) | 47 |
| AcsI | RAATTY | 2 cut(s) | 105, 510 |
| AcuI | CTGAAG | 1 cut(s) | 545 |
| AdeI | CACNNNGTG | 1 cut(s) | 748 |
| AfaI | GTAC | 5 cut(s) | 206, 266, 366, 521, 808 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 542 |
| AflII | CTTAAG | 1 cut(s) | 200 |
| AflIII | ACRYGT | 1 cut(s) | 249 |
| AgsI | TTSAA | 1 cut(s) | 796 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 628 |
| AjnI | CCWGG | 1 cut(s) | 535 |
| AluBI | AGCT | 5 cut(s) | 148, 227, 347, 380, 533 |
| AluI | AGCT | 5 cut(s) | 148, 227, 347, 380, 533 |
| Alw21I | GWGCWC | 1 cut(s) | 382 |
| Alw26I | GTCTC | 1 cut(s) | 281 |
| AlwI | GGATC | 1 cut(s) | 251 |
| Ama87I | CYCGRG | 1 cut(s) | 478 |
| AoxI | GGCC | 1 cut(s) | 47 |
| ApeKI | GCWGC | 4 cut(s) | 32, 145, 446, 836 |
| ApoI | RAATTY | 2 cut(s) | 105, 510 |
| Asp718I | GGTACC | 1 cut(s) | 364 |
| AspLEI | GCGC | 1 cut(s) | 405 |
| AsuHPI | GGTGA | 1 cut(s) | 206 |
| AvaI | CYCGRG | 1 cut(s) | 478 |
| BalI | TGGCCA | 1 cut(s) | 49 |
| BanI | GGYRCC | 1 cut(s) | 364 |
| BanII | GRGCYC | 1 cut(s) | 382 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 117, 149 |
| Bbv12I | GWGCWC | 1 cut(s) | 382 |
| BbvCI | CCTCAGC | 1 cut(s) | 717 |
| BbvI | GCAGC | 4 cut(s) | 19, 157, 458, 848 |
| BciT130I | CCWGG | 1 cut(s) | 537 |
| BciVI | GTATCC | 1 cut(s) | 363 |
| BcoDI | GTCTC | 1 cut(s) | 281 |
| BfaI | CTAG | 5 cut(s) | 36, 164, 228, 426, 711 |
| BfoI | RGCGCY | 1 cut(s) | 406 |
| BfrI | CTTAAG | 1 cut(s) | 200 |
| BfuI | GTATCC | 1 cut(s) | 363 |
| BisI | GCNGC | 4 cut(s) | 33, 146, 447, 837 |
| BlsI | GCNGC | 4 cut(s) | 34, 147, 448, 838 |
| BmcAI | AGTACT | 2 cut(s) | 206, 808 |
| Bme1390I | CCNGG | 1 cut(s) | 537 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 628 |
| BmeT110I | CYCGRG | 1 cut(s) | 478 |
| BmiI | GGNNCC | 1 cut(s) | 366 |
| BmrFI | CCNGG | 1 cut(s) | 537 |
| BmsI | GCATC | 2 cut(s) | 487, 586 |
| BpmI | CTGGAG | 1 cut(s) | 519 |
| Bpu10I | CCTNAGC | 1 cut(s) | 717 |
| BpuEI | CTTGAG | 1 cut(s) | 596 |
| BsaAI | YACGTR | 1 cut(s) | 782 |
| BsaJI | CCNNGG | 1 cut(s) | 99 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 542 |
| Bse1I | ACTGG | 2 cut(s) | 50, 860 |
| BseBI | CCWGG | 1 cut(s) | 537 |
| BseDI | CCNNGG | 1 cut(s) | 99 |
| BseGI | GGATG | 1 cut(s) | 502 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BseMII | CTCAG | 4 cut(s) | 162, 339, 708, 714 |
| BseNI | ACTGG | 2 cut(s) | 50, 860 |
| BseXI | GCAGC | 4 cut(s) | 19, 157, 458, 848 |
| BshFI | GGCC | 1 cut(s) | 49 |
| BshNI | GGYRCC | 1 cut(s) | 364 |
| BsiHKAI | GWGCWC | 1 cut(s) | 382 |
| BsiHKCI | CYCGRG | 1 cut(s) | 478 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BsmAI | GTCTC | 1 cut(s) | 281 |
| BsmI | GAATGC | 1 cut(s) | 79 |
| BsnI | GGCC | 1 cut(s) | 49 |
| BsoBI | CYCGRG | 1 cut(s) | 478 |
| Bsp1286I | GDGCHC | 1 cut(s) | 382 |
| Bsp143I | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| BspACI | CCGC | 1 cut(s) | 122 |
| BspANI | GGCC | 1 cut(s) | 49 |
| BspCNI | CTCAG | 4 cut(s) | 163, 340, 709, 715 |
| BspLI | GGNNCC | 1 cut(s) | 366 |
| BspPI | GGATC | 1 cut(s) | 251 |
| BspT107I | GGYRCC | 1 cut(s) | 364 |
| BspTI | CTTAAG | 1 cut(s) | 200 |
| BsrI | ACTGG | 2 cut(s) | 50, 860 |
| BssECI | CCNNGG | 1 cut(s) | 99 |
| BssMI | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| Bst2UI | CCWGG | 1 cut(s) | 537 |
| Bst4CI | ACNGT | 2 cut(s) | 60, 115 |
| Bst6I | CTCTTC | 1 cut(s) | 674 |
| BstAFI | CTTAAG | 1 cut(s) | 200 |
| BstBAI | YACGTR | 1 cut(s) | 782 |
| BstC8I | GCNNGC | 1 cut(s) | 382 |
| BstDEI | CTNAG | 6 cut(s) | 149, 171, 348, 717, 723, 767 |
| BstF5I | GGATG | 1 cut(s) | 502 |
| BstH2I | RGCGCY | 1 cut(s) | 406 |
| BstHHI | GCGC | 1 cut(s) | 405 |
| BstKTI | GATC | 5 cut(s) | 155, 246, 361, 710, 787 |
| BstMAI | GTCTC | 1 cut(s) | 281 |
| BstMBI | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 446, 605 |
| BstNI | CCWGG | 1 cut(s) | 537 |
| BstNSI | RCATGY | 1 cut(s) | 253 |
| BstSCI | CCNGG | 1 cut(s) | 535 |
| BstV1I | GCAGC | 4 cut(s) | 19, 157, 458, 848 |
| BstX2I | RGATCY | 1 cut(s) | 243 |
| BstYI | RGATCY | 1 cut(s) | 243 |
| BsuI | GTATCC | 1 cut(s) | 363 |
| BsuRI | GGCC | 1 cut(s) | 49 |
| BtsCI | GGATG | 1 cut(s) | 502 |
| Cac8I | GCNNGC | 1 cut(s) | 382 |
| CfoI | GCGC | 1 cut(s) | 405 |
| Csp6I | GTAC | 5 cut(s) | 205, 265, 365, 520, 807 |
| CviAII | CATG | 5 cut(s) | 86, 119, 250, 469, 675 |
| CviQI | GTAC | 5 cut(s) | 205, 265, 365, 520, 807 |
| DdeI | CTNAG | 6 cut(s) | 149, 171, 348, 717, 723, 767 |
| DpnI | GATC | 5 cut(s) | 154, 245, 360, 709, 786 |
| DpnII | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| DraI | TTTAAA | 2 cut(s) | 42, 516 |
| DraIII | CACNNNGTG | 1 cut(s) | 748 |
| DriI | GACNNNNNGTC | 1 cut(s) | 628 |
| EaeI | YGGCCR | 1 cut(s) | 47 |
| Eam1104I | CTCTTC | 1 cut(s) | 674 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 628 |
| EarI | CTCTTC | 1 cut(s) | 674 |
| Ecl136II | GAGCTC | 1 cut(s) | 380 |
| Eco24I | GRGCYC | 1 cut(s) | 382 |
| Eco53kI | GAGCTC | 1 cut(s) | 380 |
| Eco57I | CTGAAG | 1 cut(s) | 545 |
| Eco88I | CYCGRG | 1 cut(s) | 478 |
| EcoICRI | GAGCTC | 1 cut(s) | 380 |
| EcoRII | CCWGG | 1 cut(s) | 535 |
| EcoT22I | ATGCAT | 2 cut(s) | 79, 474 |
| EcoT38I | GRGCYC | 1 cut(s) | 382 |
| FaeI | CATG | 5 cut(s) | 89, 122, 253, 472, 678 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 194, 226 |
| FatI | CATG | 5 cut(s) | 85, 118, 249, 468, 674 |
| FauNDI | CATATG | 2 cut(s) | 442, 492 |
| Fnu4HI | GCNGC | 4 cut(s) | 33, 146, 447, 837 |
| FokI | GGATG | 1 cut(s) | 509 |
| FriOI | GRGCYC | 1 cut(s) | 382 |
| Fsp4HI | GCNGC | 4 cut(s) | 33, 146, 447, 837 |
| FspBI | CTAG | 5 cut(s) | 36, 164, 228, 426, 711 |
| GlaI | GCGC | 1 cut(s) | 404 |
| GluI | GCNGC | 4 cut(s) | 33, 146, 447, 837 |
| GsuI | CTGGAG | 1 cut(s) | 519 |
| HaeII | RGCGCY | 1 cut(s) | 406 |
| HaeIII | GGCC | 1 cut(s) | 49 |
| HhaI | GCGC | 1 cut(s) | 405 |
| Hin1II | CATG | 5 cut(s) | 89, 122, 253, 472, 678 |
| Hin6I | GCGC | 1 cut(s) | 403 |
| HinP1I | GCGC | 1 cut(s) | 403 |
| HincII | GTYRAC | 1 cut(s) | 633 |
| HindII | GTYRAC | 1 cut(s) | 633 |
| HinfI | GANTC | 2 cut(s) | 334, 856 |
| HphI | GGTGA | 1 cut(s) | 206 |
| Hpy166II | GTNNAC | 2 cut(s) | 197, 633 |
| Hpy188I | TCNGA | 3 cut(s) | 56, 172, 390 |
| Hpy188III | TCNNGA | 3 cut(s) | 70, 272, 356 |
| Hpy8I | GTNNAC | 2 cut(s) | 197, 633 |
| HpyAV | CCTTC | 4 cut(s) | 119, 170, 233, 534 |
| HpyCH4III | ACNGT | 2 cut(s) | 60, 115 |
| HpyCH4IV | ACGT | 2 cut(s) | 267, 781 |
| HpyCH4V | TGCA | 7 cut(s) | 77, 306, 440, 446, 472, 599, 814 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 446, 605 |
| HpyF3I | CTNAG | 6 cut(s) | 149, 171, 348, 717, 723, 767 |
| HpySE526I | ACGT | 2 cut(s) | 267, 781 |
| Hsp92II | CATG | 5 cut(s) | 89, 122, 253, 472, 678 |
| HspAI | GCGC | 1 cut(s) | 403 |
| KpnI | GGTACC | 1 cut(s) | 368 |
| Kzo9I | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| LmnI | GCTCC | 1 cut(s) | 538 |
| Lsp1109I | GCAGC | 4 cut(s) | 19, 157, 458, 848 |
| LweI | GCATC | 2 cut(s) | 487, 586 |
| MaeI | CTAG | 5 cut(s) | 36, 164, 228, 426, 711 |
| MaeII | ACGT | 2 cut(s) | 267, 781 |
| MaeIII | GTNAC | 5 cut(s) | 132, 641, 670, 777, 824 |
| MalI | GATC | 5 cut(s) | 154, 245, 360, 709, 786 |
| MboI | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| MboII | GAAGA | 2 cut(s) | 74, 691 |
| MflI | RGATCY | 1 cut(s) | 243 |
| MhlI | GDGCHC | 1 cut(s) | 382 |
| MlsI | TGGCCA | 1 cut(s) | 49 |
| MluCI | AATT | 5 cut(s) | 105, 216, 510, 664, 755 |
| MluNI | TGGCCA | 1 cut(s) | 49 |
| MmeI | TCCRAC | 2 cut(s) | 79, 279 |
| MnlI | CCTC | 9 cut(s) | 94, 178, 214, 252, 384, 571, 621, 712, 725 |
| Mox20I | TGGCCA | 1 cut(s) | 49 |
| Mph1103I | ATGCAT | 2 cut(s) | 79, 474 |
| MscI | TGGCCA | 1 cut(s) | 49 |
| MseI | TTAA | 3 cut(s) | 41, 201, 515 |
| MslI | CAYNNNNRTG | 1 cut(s) | 495 |
| Msp20I | TGGCCA | 1 cut(s) | 49 |
| MspCI | CTTAAG | 1 cut(s) | 200 |
| MspR9I | CCNGG | 1 cut(s) | 537 |
| MssI | GTTTAAAC | 1 cut(s) | 42 |
| Mva1269I | GAATGC | 1 cut(s) | 79 |
| MvaI | CCWGG | 1 cut(s) | 537 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 446, 605 |
| NdeI | CATATG | 2 cut(s) | 442, 492 |
| NdeII | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| NlaIII | CATG | 5 cut(s) | 89, 122, 253, 472, 678 |
| NlaIV | GGNNCC | 1 cut(s) | 366 |
| NmeAIII | GCCGAG | 1 cut(s) | 124 |
| NmuCI | GTSAC | 1 cut(s) | 824 |
| NsiI | ATGCAT | 2 cut(s) | 79, 474 |
| NspI | RCATGY | 1 cut(s) | 253 |
| PciI | ACATGT | 1 cut(s) | 249 |
| PctI | GAATGC | 1 cut(s) | 79 |
| PfeI | GAWTC | 2 cut(s) | 334, 856 |
| PfoI | TCCNGGA | 1 cut(s) | 535 |
| PkrI | GCNGC | 4 cut(s) | 34, 147, 448, 838 |
| PmeI | GTTTAAAC | 1 cut(s) | 42 |
| Ppu21I | YACGTR | 1 cut(s) | 782 |
| PscI | ACATGT | 1 cut(s) | 249 |
| Psp124BI | GAGCTC | 1 cut(s) | 382 |
| Psp6I | CCWGG | 1 cut(s) | 535 |
| PspGI | CCWGG | 1 cut(s) | 535 |
| PspN4I | GGNNCC | 1 cut(s) | 366 |
| PsuI | RGATCY | 1 cut(s) | 243 |
| RsaI | GTAC | 5 cut(s) | 206, 266, 366, 521, 808 |
| RsaNI | GTAC | 5 cut(s) | 205, 265, 365, 520, 807 |
| RseI | CAYNNNNRTG | 1 cut(s) | 495 |
| SacI | GAGCTC | 1 cut(s) | 382 |
| SaqAI | TTAA | 3 cut(s) | 41, 201, 515 |
| SatI | GCNGC | 4 cut(s) | 33, 146, 447, 837 |
| Sau3AI | GATC | 5 cut(s) | 152, 243, 358, 707, 784 |
| ScaI | AGTACT | 2 cut(s) | 206, 808 |
| ScrFI | CCNGG | 1 cut(s) | 537 |
| SduI | GDGCHC | 1 cut(s) | 382 |
| SfaNI | GCATC | 2 cut(s) | 487, 586 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 495 |
| SmlI | CTYRAG | 2 cut(s) | 200, 575 |
| SmoI | CTYRAG | 2 cut(s) | 200, 575 |
| Sse9I | AATT | 5 cut(s) | 105, 216, 510, 664, 755 |
| SsiI | CCGC | 1 cut(s) | 122 |
| SspI | AATATT | 1 cut(s) | 616 |
| SspMI | CTAG | 5 cut(s) | 36, 164, 228, 426, 711 |
| SstI | GAGCTC | 1 cut(s) | 382 |
| StyD4I | CCNGG | 1 cut(s) | 535 |
| TaaI | ACNGT | 2 cut(s) | 60, 115 |
| TaiI | ACGT | 2 cut(s) | 270, 784 |
| TasI | AATT | 5 cut(s) | 105, 216, 510, 664, 755 |
| TatI | WGTACW | 2 cut(s) | 204, 806 |
| TfiI | GAWTC | 2 cut(s) | 334, 856 |
| Tru1I | TTAA | 3 cut(s) | 41, 201, 515 |
| Tru9I | TTAA | 3 cut(s) | 41, 201, 515 |
| TseFI | GTSAC | 1 cut(s) | 824 |
| TseI | GCWGC | 4 cut(s) | 32, 145, 446, 836 |
| Tsp45I | GTSAC | 1 cut(s) | 824 |
| TspDTI | ATGAA | 5 cut(s) | 17, 74, 107, 197, 457 |
| TspGWI | ACGGA | 1 cut(s) | 759 |
| Vha464I | CTTAAG | 1 cut(s) | 200 |
| XapI | RAATTY | 2 cut(s) | 105, 510 |
| XceI | RCATGY | 1 cut(s) | 253 |
| XspI | CTAG | 5 cut(s) | 36, 164, 228, 426, 711 |
| ZrmI | AGTACT | 2 cut(s) | 206, 808 |
| Zsp2I | ATGCAT | 2 cut(s) | 79, 474 |