MD05G1047900.v1.1

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000071
Physical Location & Seq
Forward (+)
8152500 .. 8157677
5178 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1047900.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGAGAAAATAGACGCCCTCAGTGGCAGCGAGGGTTCTGGCGGTGACGGTTCTGTTTGTGGATATGACTCATTTCACCGCCTCTTGAACGCCAATCTCAAACCCCATCTCTTCCAGATCATTGGGCGTGGTACTAAGAGAGATGGGCTATATTATGTGGATGACGTGGTTCCTAGAAGAGTTAATTTGGTTCATGCATCACGTAATAGTAATCTACAGAAGGTATTGTTATTGCATTGTCGTTTGAGGCATGCATCGTTTGGTTATTTGAAACATATATTACCTACTTTGTTCAGTGGTATTGAAGACTCAGAATTGAAATGTGAAGTATGTATTTTAGCCAAAAGTCATCGTGCTTCATTCCCTCCAAGTATGAATAGAACATCTTCTCCATTTGCGCTAGTACACTCTGATGTGTGGAGGCCTTCCCTTATTAGTACTACTTCGGGAATTAAATGGTTTATTACGTGTGTTTTTGTGGGTTTTGCATCCCACAAGAAAGGTTACAAGTGTTACCACCCGGCAACTCGTTACATATATATAACCATGGATGTGACTTTCTCTGAGTTGGAATATTTTTATGCTCTCGTATCACCACATTTCGATCATAATGGGGAGAGTTCTAGTTGTGATTTTGAATGGTTGGATGTACAAGAGGTGACGGTTTTAGGGGGAATGTACGGTGCTGTGTCGAGTGATGCAGTTGATGCTCGGTTTCCTCTTGCTGAGCCTGTTCTTCTCACCGAAGAAGAGGCAGCCAGTCCGTGTGTTGGTGCTATCGAAGATGAGTGCATTGATGGTGATGTGACTGATAGGTGCTCTACTTCTGGATACTTTACGCTTGTTGGCGGAAATCTGGTTACTTGGATAAGCAAGAAGCAAAATGTGGTGTCAAGGTCTTCAGCTGAGGCCGACTTCAGAGGTATGGCACATGGAGTATGTGAAGTTCTTTGGTTACGTAAGTTACTTTGGGGTCTAGGTTTCAAAGCAAAAGTGGCCATAAGCTTGTATTGTGATAATAAATCCGCATGGGAGATTGCAGAGAACCCGGTACAACATGATAGAACAAAGCATGTGGAGGTTGATCGTCATTTTATTAAGAAAAAGCTTGAGAAGAAGATTGTGTCAATACCGTTTGTGAACTCGGAAGAACAGATTGTGTCAATACCGTGTGGTTTGAATCGCGACAAGGCACTTGAAACAATTTCTCTCCCAGAATCAGCTAAAGCTTTCTCTTCTGGACATGATTTTGACCTCCAGGTAGGAAGTTATACTGTCATTTTTTTTGGCTATGCCATGCTGCTACCATTCACATACGAGCAATTCACAATCAACCGAATACAAATTCAGCCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0001889 GO:0003674 GO:0003824 GO:0003837 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006206 GO:0006207 GO:0006208 GO:0006210 GO:0006212 GO:0006213 GO:0006520 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0008270 GO:0008652 GO:0009056 GO:0009058 GO:0009112 GO:0009116 GO:0009164 GO:0009605 GO:0009987 GO:0009991 GO:0016053 GO:0016787 GO:0016810 GO:0016811 GO:0017144 GO:0018130 GO:0019439 GO:0019482 GO:0019483 GO:0019752 GO:0019856 GO:0019859 GO:0019860 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033396 GO:0034641 GO:0034655 GO:0034656 GO:0042133 GO:0042136 GO:0042737 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043292 GO:0043436 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044444 GO:0044449 GO:0044464 GO:0046112 GO:0046113 GO:0046135 GO:0046394 GO:0046483 GO:0046700 GO:0046872 GO:0046914 GO:0048513 GO:0048731 GO:0048732 GO:0048856 GO:0050896 GO:0051716 GO:0055086 GO:0055120 GO:0061008 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0072527 GO:0072528 GO:0072529 GO:0099080 GO:0099081 GO:0099512 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

453

Amino Acids

50.52

Weight (kDa)

6.28

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 49 - 116 1.5e-09 GAG-pre-integrase domain
SH3_retrovirus PF25597 156 - 193 3.8e-11 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1185
AciI CCGC 4 cut(s) 42, 79, 849, 1026
AcoI YGGCCR 1 cut(s) 996
AcsI RAATTY 1 cut(s) 1344
AcuI CTGAAG 2 cut(s) 885, 901
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 6 cut(s) 133, 405, 439, 651, 680, 1053
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 8 cut(s) 88, 271, 305, 319, 638, 985, 1180, 1199
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 1257
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 5 cut(s) 905, 1005, 1108, 1223, 1229
AluI AGCT 5 cut(s) 905, 1005, 1108, 1223, 1229
Alw21I GWGCWC 1 cut(s) 821
AoxI GGCC 3 cut(s) 422, 909, 996
ApeKI GCWGC 3 cut(s) 27, 755, 1300
ApoI RAATTY 1 cut(s) 1344
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 400
AsuC2I CCSGG 2 cut(s) 521, 1049
AsuHPI GGTGA 6 cut(s) 56, 68, 585, 670, 733, 812
BalI TGGCCA 1 cut(s) 998
BbsI GAAGAC 2 cut(s) 312, 891
Bbv12I GWGCWC 1 cut(s) 821
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 3 cut(s) 39, 767, 1287
BccI CCATC 3 cut(s) 114, 137, 791
BciT130I CCWGG 1 cut(s) 1259
BciVI GTATCC 1 cut(s) 824
BcnI CCSGG 2 cut(s) 521, 1049
BfaI CTAG 4 cut(s) 174, 401, 624, 977
BfmI CTRYAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 824
BisI GCNGC 3 cut(s) 28, 756, 1301
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 3 cut(s) 29, 757, 1302
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 3 cut(s) 521, 1049, 1259
BmgBI CACGTC 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 3 cut(s) 521, 1049, 1259
BmsI GCATC 5 cut(s) 206, 263, 497, 688, 697
BpiI GAAGAC 2 cut(s) 312, 891
BpmI CTGGAG 1 cut(s) 1241
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 2 cut(s) 521, 1049
BsaAI YACGTR 3 cut(s) 203, 468, 959
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 1 cut(s) 759
BseBI CCWGG 1 cut(s) 1259
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 4 cut(s) 166, 488, 556, 652
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 5 cut(s) 34, 324, 555, 717, 897
BseNI ACTGG 1 cut(s) 759
BseXI GCAGC 3 cut(s) 39, 767, 1287
Bsh1236I CGCG 1 cut(s) 1185
BshFI GGCC 3 cut(s) 424, 911, 998
BsiHKAI GWGCWC 1 cut(s) 821
BsiSI CCGG 2 cut(s) 521, 1049
BslI CCNNNNNNNGG 1 cut(s) 770
BsnI GGCC 3 cut(s) 424, 911, 998
Bsp1286I GDGCHC 1 cut(s) 821
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 3 cut(s) 117, 604, 1084
Bsp1720I GCTNAGC 1 cut(s) 726
Bsp19I CCATGG 1 cut(s) 546
Bsp68I TCGCGA 1 cut(s) 1185
BspACI CCGC 4 cut(s) 42, 79, 849, 1026
BspANI GGCC 3 cut(s) 424, 911, 998
BspCNI CTCAG 5 cut(s) 33, 323, 556, 718, 898
BspFNI CGCG 1 cut(s) 1185
BspLI GGNNCC 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 1 cut(s) 759
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 3 cut(s) 117, 604, 1084
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 1259
Bst4CI ACNGT 6 cut(s) 50, 664, 683, 1134, 1170, 1276
Bst6I CTCTTC 4 cut(s) 116, 172, 744, 1240
BstACI GRCGYC 1 cut(s) 15
BstAPI GCANNNNNTGC 1 cut(s) 707
BstAUI TGTACA 1 cut(s) 649
BstBAI YACGTR 3 cut(s) 203, 468, 959
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 6 cut(s) 20, 135, 310, 564, 726, 906
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 4 cut(s) 166, 488, 556, 652
BstFNI CGCG 1 cut(s) 1185
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 3 cut(s) 120, 607, 1087
BstMBI GATC 3 cut(s) 117, 604, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 707, 995
BstNI CCWGG 1 cut(s) 1259
BstNSI RCATGY 2 cut(s) 254, 1076
BstSCI CCNGG 3 cut(s) 519, 1047, 1257
BstSFI CTRYAG 1 cut(s) 215
BstSNI TACGTA 1 cut(s) 959
BstUI CGCG 1 cut(s) 1185
BstV1I GCAGC 3 cut(s) 39, 767, 1287
BstV2I GAAGAC 2 cut(s) 312, 891
BstXI CCANNNNNNTGG 1 cut(s) 122
BsuI GTATCC 1 cut(s) 824
BsuRI GGCC 3 cut(s) 424, 911, 998
BtgI CCRYGG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 4 cut(s) 166, 488, 556, 652
BtsIMutI CAGTG 2 cut(s) 28, 301
BtuMI TCGCGA 1 cut(s) 1185
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 1 cut(s) 400
CseI GACGC 1 cut(s) 23
Csp6I GTAC 6 cut(s) 132, 404, 438, 650, 679, 1052
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 9 cut(s) 194, 251, 547, 932, 1029, 1058, 1073, 1244, 1297
CviQI GTAC 6 cut(s) 132, 404, 438, 650, 679, 1052
DdeI CTNAG 6 cut(s) 20, 135, 310, 564, 726, 906
DpnI GATC 3 cut(s) 119, 606, 1086
DpnII GATC 3 cut(s) 117, 604, 1084
EaeI YGGCCR 1 cut(s) 996
Eam1104I CTCTTC 4 cut(s) 116, 172, 744, 1240
EarI CTCTTC 4 cut(s) 116, 172, 744, 1240
EciI GGCGGA 1 cut(s) 864
Eco105I TACGTA 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 546
Eco147I AGGCCT 1 cut(s) 424
Eco57I CTGAAG 2 cut(s) 885, 901
EcoRII CCWGG 1 cut(s) 1257
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 2 cut(s) 199, 256
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 9 cut(s) 197, 254, 550, 935, 1032, 1061, 1076, 1247, 1300
FatI CATG 9 cut(s) 193, 250, 546, 931, 1028, 1057, 1072, 1243, 1296
Fnu4HI GCNGC 3 cut(s) 28, 756, 1301
FokI GGATG 4 cut(s) 173, 475, 563, 659
Fsp4HI GCNGC 3 cut(s) 28, 756, 1301
FspBI CTAG 4 cut(s) 174, 401, 624, 977
GlaI GCGC 1 cut(s) 399
GluI GCNGC 3 cut(s) 28, 756, 1301
GsuI CTGGAG 1 cut(s) 1241
HaeIII GGCC 3 cut(s) 424, 911, 998
HapII CCGG 2 cut(s) 521, 1049
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 9 cut(s) 197, 254, 550, 935, 1032, 1061, 1076, 1247, 1300
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HindIII AAGCTT 3 cut(s) 1003, 1106, 1227
HinfI GANTC 4 cut(s) 68, 308, 1180, 1217
HpaII CCGG 2 cut(s) 521, 1049
HphI GGTGA 6 cut(s) 56, 68, 585, 670, 733, 812
Hpy166II GTNNAC 2 cut(s) 406, 1141
Hpy188I TCNGA 5 cut(s) 313, 412, 565, 920, 1147
Hpy188III TCNNGA 6 cut(s) 85, 115, 447, 828, 1184, 1239
Hpy8I GTNNAC 2 cut(s) 406, 1141
HpyAV CCTTC 2 cut(s) 214, 435
HpyCH4III ACNGT 6 cut(s) 50, 664, 683, 1134, 1170, 1276
HpyCH4IV ACGT 4 cut(s) 165, 202, 467, 958
HpyCH4V TGCA 7 cut(s) 197, 235, 254, 488, 701, 792, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 707, 995
HpyF3I CTNAG 6 cut(s) 20, 135, 310, 564, 726, 906
HpySE526I ACGT 4 cut(s) 165, 202, 467, 958
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 9 cut(s) 197, 254, 550, 935, 1032, 1061, 1076, 1247, 1300
HspAI GCGC 1 cut(s) 398
Kzo9I GATC 3 cut(s) 117, 604, 1084
Lsp1109I GCAGC 3 cut(s) 39, 767, 1287
LweI GCATC 5 cut(s) 206, 263, 497, 688, 697
MaeI CTAG 4 cut(s) 174, 401, 624, 977
MaeII ACGT 4 cut(s) 165, 202, 467, 958
MalI GATC 3 cut(s) 119, 606, 1086
MboI GATC 3 cut(s) 117, 604, 1084
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 998
MluCI AATT 6 cut(s) 184, 314, 450, 1203, 1322, 1344
MluNI TGGCCA 1 cut(s) 998
MlyI GAGTC 2 cut(s) 62, 302
MmeI TCCRAC 2 cut(s) 549, 624
Mox20I TGGCCA 1 cut(s) 998
Mph1103I ATGCAT 2 cut(s) 199, 256
MroXI GAANNNNTTC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 998
MseI TTAA 3 cut(s) 183, 453, 1098
MslI CAYNNNNRTG 2 cut(s) 411, 551
Msp20I TGGCCA 1 cut(s) 998
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 2 cut(s) 521, 1049
MspR9I CCNGG 3 cut(s) 521, 1049, 1259
MvaI CCWGG 1 cut(s) 1259
MvnI CGCG 1 cut(s) 1185
MwoI GCNNNNNNNGC 2 cut(s) 707, 995
NciI CCSGG 2 cut(s) 521, 1049
NcoI CCATGG 1 cut(s) 546
NdeII GATC 3 cut(s) 117, 604, 1084
NlaIII CATG 9 cut(s) 197, 254, 550, 935, 1032, 1061, 1076, 1247, 1300
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 4 cut(s) 44, 553, 658, 805
NruI TCGCGA 1 cut(s) 1185
NsiI ATGCAT 2 cut(s) 199, 256
NspI RCATGY 2 cut(s) 254, 1076
PaeI GCATGC 1 cut(s) 254
PceI AGGCCT 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 2 cut(s) 1180, 1217
PkrI GCNGC 3 cut(s) 29, 757, 1302
PleI GAGTC 2 cut(s) 62, 302
PpsI GAGTC 2 cut(s) 62, 302
Ppu21I YACGTR 3 cut(s) 203, 468, 959
Psp6I CCWGG 1 cut(s) 1257
PspGI CCWGG 1 cut(s) 1257
PspN4I GGNNCC 1 cut(s) 171
PsrI GAACNNNNNNTAC 2 cut(s) 930, 962
PvuII CAGCTG 1 cut(s) 905
RruI TCGCGA 1 cut(s) 1185
RsaI GTAC 6 cut(s) 133, 405, 439, 651, 680, 1053
RsaNI GTAC 6 cut(s) 132, 404, 438, 650, 679, 1052
RseI CAYNNNNRTG 2 cut(s) 411, 551
SaqAI TTAA 3 cut(s) 183, 453, 1098
SatI GCNGC 3 cut(s) 28, 756, 1301
Sau3AI GATC 3 cut(s) 117, 604, 1084
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 2 cut(s) 62, 302
ScrFI CCNGG 3 cut(s) 521, 1049, 1259
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 5 cut(s) 206, 263, 497, 688, 697
SfcI CTRYAG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 411, 551
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
SnaBI TACGTA 1 cut(s) 959
SphI GCATGC 1 cut(s) 254
Sse9I AATT 6 cut(s) 184, 314, 450, 1203, 1322, 1344
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 4 cut(s) 42, 79, 849, 1026
SspI AATATT 1 cut(s) 575
SspMI CTAG 4 cut(s) 174, 401, 624, 977
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 3 cut(s) 519, 1047, 1257
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 6 cut(s) 50, 664, 683, 1134, 1170, 1276
TaiI ACGT 4 cut(s) 168, 205, 470, 961
TaqI TCGA 3 cut(s) 603, 692, 780
TasI AATT 6 cut(s) 184, 314, 450, 1203, 1322, 1344
TatI WGTACW 3 cut(s) 403, 437, 649
TfiI GAWTC 2 cut(s) 1180, 1217
Tru1I TTAA 3 cut(s) 183, 453, 1098
Tru9I TTAA 3 cut(s) 183, 453, 1098
TscAI CASTG 2 cut(s) 28, 301
TseFI GTSAC 4 cut(s) 44, 553, 658, 805
TseI GCWGC 3 cut(s) 27, 755, 1300
Tsp45I GTSAC 4 cut(s) 44, 553, 658, 805
TspDTI ATGAA 3 cut(s) 182, 348, 389
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 2 cut(s) 28, 301
XapI RAATTY 1 cut(s) 1344
XceI RCATGY 2 cut(s) 254, 1076
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 4 cut(s) 174, 401, 624, 977
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 2 cut(s) 199, 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.