RchiOBHm_Chr2g0113601
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Reverse (-)
25738976 .. 25740418
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48694

Sequence Viewer

Length: 960 bp
ATGGTGAAAGATCAAGACGTTTCTCCCAGGTTCTCCTCAAATCTGACTTGCAACTACTGCAAGAATCCAGGCCACATCAAAGCAAGTTGTTACAGGTTGGTTGGTTTTCCAGCAGGCTACTTTGATAGGCCCCGACGTCAGGACAACAAACCCAAGGGAAAGGCTGTTGTTCATCTTGTTCAAGACCAAGATTACTCTGCGGTGACAAAACCAGATCACACCAACTTTGCTGGTAAGGATACCGCATCCATAAGTCGTGGTGGTAATGGTAAGGTTGGAGCAGCTTTAAAATTTTCTAGCTTTACTGGTGGCAATACTTGGATAATTGATTCTGGAGCATCTGACCATATGACCTACGATCGTAGTTTCTTTCTTTTTCTCAATCCACCCTCTATTTCCTCTGTTGTCAATGCCAATGGTGATTCTTTTCCTGTTTTAGGAATAGGGTCTATTCGTCTCACCCCTTCTTTGACACTACATGATGTTCTTTATGTCCCTGATCTCTCTCATCATCTTATATCTGTGCCTCAACTTAATACTCAATCTCGGTGTTCTGTAACATTTTTTCCGTTGTATGTGATTTTTCAGGACCTCCTCACCAGGGAAATAATTGGCAGGGGTTATCTGAGGGGTCGACTATTTCATCTGGATTGCATGTTCGCCGGAGTGAAGCCGGAGAAGGCAGTTCAAGCAGCTTTGATTTCGACTGGGGTTACTAATCAAGTTGAAGAATTATGGCTATGGCATCGGAGATTGGGACATCCTTCCTTTAGTGTCATGAAGAAATCTATGCCATCCTTGTTTCTGGGTATTGATGAGTCTAAGTTGCACTTTCTTTTCCTTTTGAGCTTATTCATTCTGATATCTGGGGACCTTCCCGTGAGTCAACCTTGTCTGGAAAACATTGGTTTGTTTTGTTTATTGATGATTATACCCGTCTTACTTGGGTCGCTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

319

Amino Acids

35.34

Weight (kDa)

8.92

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 107 - 180 1.6e-19 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 235 - 276 2.9e-10 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 634
AciI CCGC 2 cut(s) 200, 243
AcsI RAATTY 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 139, 437, 601
AgsI TTSAA 3 cut(s) 182, 689, 728
AjnI CCWGG 3 cut(s) 26, 67, 599
AloI GAACNNNNNNTCC 2 cut(s) 641, 673
AluBI AGCT 4 cut(s) 284, 300, 695, 849
AluI AGCT 4 cut(s) 284, 300, 695, 849
Alw26I GTCTC 1 cut(s) 461
AoxI GGCC 2 cut(s) 70, 128
ApeKI GCWGC 2 cut(s) 281, 692
ApoI RAATTY 1 cut(s) 290
AspS9I GGNCC 3 cut(s) 129, 589, 871
AsuHPI GGTGA 5 cut(s) 16, 214, 431, 451, 589
AvaII GGWCC 2 cut(s) 589, 871
BbvI GCAGC 2 cut(s) 293, 704
BccI CCATC 1 cut(s) 802
BciT130I CCWGG 3 cut(s) 28, 69, 601
BciVI GTATCC 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 2 cut(s) 282, 693
BlsI GCNGC 2 cut(s) 283, 694
Bme1390I CCNGG 3 cut(s) 28, 69, 601
Bme18I GGWCC 2 cut(s) 589, 871
BmgT120I GGNCC 3 cut(s) 129, 589, 871
BmiI GGNNCC 2 cut(s) 131, 872
BmrFI CCNGG 3 cut(s) 28, 69, 601
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 3 cut(s) 254, 347, 754
BmuI ACTGGG 1 cut(s) 717
BpmI CTGGAG 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 26, 153, 600
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 437, 601
Bse1I ACTGG 2 cut(s) 310, 712
BseBI CCWGG 3 cut(s) 28, 69, 601
BseDI CCNNGG 3 cut(s) 26, 153, 600
BseGI GGATG 3 cut(s) 245, 760, 794
BseLI CCNNNNNNNGG 3 cut(s) 139, 437, 601
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 2 cut(s) 310, 712
BseRI GAGGAG 2 cut(s) 25, 584
BseXI GCAGC 2 cut(s) 293, 704
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 2 cut(s) 72, 130
BsiEI CGRYCG 1 cut(s) 361
BsiSI CCGG 2 cut(s) 663, 674
BslFI GGGAC 3 cut(s) 479, 771, 884
BslI CCNNNNNNNGG 3 cut(s) 139, 437, 601
BsmAI GTCTC 1 cut(s) 461
BsmBI CGTCTC 1 cut(s) 461
BsmFI GGGAC 3 cut(s) 479, 771, 884
BsnI GGCC 2 cut(s) 72, 130
Bsp143I GATC 4 cut(s) 10, 214, 358, 499
BspACI CCGC 2 cut(s) 200, 243
BspANI GGCC 2 cut(s) 72, 130
BspCNI CTCAG 1 cut(s) 618
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 131, 872
BsrI ACTGG 2 cut(s) 310, 712
BssECI CCNNGG 3 cut(s) 26, 153, 600
BssMI GATC 4 cut(s) 10, 214, 358, 499
BssNI GRCGYC 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 3 cut(s) 28, 69, 601
BstACI GRCGYC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 626, 822
BstENI CCTNNNNNAGG 1 cut(s) 435
BstF5I GGATG 3 cut(s) 245, 760, 794
BstKTI GATC 4 cut(s) 13, 217, 361, 502
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 4 cut(s) 10, 214, 358, 499
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 2 cut(s) 57, 689
BstNI CCWGG 3 cut(s) 28, 69, 601
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 3 cut(s) 26, 67, 599
BstV1I GCAGC 2 cut(s) 293, 704
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 72, 130
BtsCI GGATG 3 cut(s) 245, 760, 794
Cac8I GCNNGC 1 cut(s) 115
CciI TCATGA 1 cut(s) 777
Cfr13I GGNCC 3 cut(s) 129, 589, 871
CviAII CATG 3 cut(s) 479, 655, 778
DdeI CTNAG 2 cut(s) 626, 822
DpnI GATC 4 cut(s) 12, 216, 360, 501
DpnII GATC 4 cut(s) 10, 214, 358, 499
DraI TTTAAA 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 153
Eco32I GATATC 1 cut(s) 864
Eco47I GGWCC 2 cut(s) 589, 871
EcoNI CCTNNNNNAGG 1 cut(s) 435
EcoO109I RGGNCCY 3 cut(s) 129, 589, 871
EcoRII CCWGG 3 cut(s) 26, 67, 599
EcoRV GATATC 1 cut(s) 864
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 3 cut(s) 482, 658, 781
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 3 cut(s) 479, 771, 884
FatI CATG 3 cut(s) 478, 654, 777
FauNDI CATATG 1 cut(s) 348
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 282, 693
FokI GGATG 3 cut(s) 232, 747, 781
Fsp4HI GCNGC 2 cut(s) 282, 693
FspBI CTAG 1 cut(s) 297
GluI GCNGC 2 cut(s) 282, 693
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 2 cut(s) 72, 130
HapII CCGG 2 cut(s) 663, 674
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 3 cut(s) 482, 658, 781
HincII GTYRAC 2 cut(s) 635, 887
HindII GTYRAC 2 cut(s) 635, 887
HinfI GANTC 5 cut(s) 64, 329, 422, 818, 883
HpaII CCGG 2 cut(s) 663, 674
HphI GGTGA 5 cut(s) 16, 214, 431, 451, 589
Hpy166II GTNNAC 2 cut(s) 635, 887
Hpy188I TCNGA 5 cut(s) 45, 343, 627, 750, 861
Hpy188III TCNNGA 8 cut(s) 14, 140, 182, 333, 587, 647, 778, 896
Hpy8I GTNNAC 2 cut(s) 635, 887
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 4 cut(s) 474, 673, 774, 884
HpyCH4IV ACGT 2 cut(s) 18, 136
HpyCH4V TGCA 4 cut(s) 51, 60, 654, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 689
HpyF3I CTNAG 2 cut(s) 626, 822
HpySE526I ACGT 2 cut(s) 18, 136
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 3 cut(s) 482, 658, 781
Kzo9I GATC 4 cut(s) 10, 214, 358, 499
LmnI GCTCC 2 cut(s) 278, 335
Lsp1109I GCAGC 2 cut(s) 293, 704
LweI GCATC 3 cut(s) 254, 347, 754
MaeI CTAG 1 cut(s) 297
MaeII ACGT 2 cut(s) 18, 136
MaeIII GTNAC 4 cut(s) 89, 202, 556, 712
MalI GATC 4 cut(s) 12, 216, 360, 501
MboI GATC 4 cut(s) 10, 214, 358, 499
MboII GAAGA 2 cut(s) 740, 793
MluCI AATT 4 cut(s) 290, 324, 609, 731
MlyI GAGTC 2 cut(s) 827, 892
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 7 cut(s) 46, 400, 409, 537, 602, 605, 621
MseI TTAA 2 cut(s) 287, 534
MspI CCGG 2 cut(s) 663, 674
MspR9I CCNGG 3 cut(s) 28, 69, 601
MvaI CCWGG 3 cut(s) 28, 69, 601
MwoI GCNNNNNNNGC 2 cut(s) 57, 689
NdeI CATATG 1 cut(s) 348
NdeII GATC 4 cut(s) 10, 214, 358, 499
NlaIII CATG 3 cut(s) 482, 658, 781
NlaIV GGNNCC 2 cut(s) 131, 872
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 658
PagI TCATGA 1 cut(s) 777
PfeI GAWTC 3 cut(s) 64, 329, 422
PkrI GCNGC 2 cut(s) 283, 694
Ple19I CGATCG 1 cut(s) 361
PleI GAGTC 2 cut(s) 826, 891
PpsI GAGTC 2 cut(s) 826, 891
PpuMI RGGWCCY 2 cut(s) 589, 871
Psp5II RGGWCCY 2 cut(s) 589, 871
Psp6I CCWGG 3 cut(s) 26, 67, 599
PspGI CCWGG 3 cut(s) 26, 67, 599
PspN4I GGNNCC 2 cut(s) 131, 872
PspPI GGNCC 3 cut(s) 129, 589, 871
PspPPI RGGWCCY 2 cut(s) 589, 871
PvuI CGATCG 1 cut(s) 361
SalI GTCGAC 1 cut(s) 633
SaqAI TTAA 2 cut(s) 287, 534
SatI GCNGC 2 cut(s) 282, 693
Sau3AI GATC 4 cut(s) 10, 214, 358, 499
Sau96I GGNCC 3 cut(s) 129, 589, 871
SchI GAGTC 2 cut(s) 827, 892
ScrFI CCNGG 3 cut(s) 28, 69, 601
SfaNI GCATC 3 cut(s) 254, 347, 754
SinI GGWCC 2 cut(s) 589, 871
Sse9I AATT 4 cut(s) 290, 324, 609, 731
SsiI CCGC 2 cut(s) 200, 243
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 26, 67, 599
StyI CCWWGG 1 cut(s) 153
TaiI ACGT 2 cut(s) 21, 139
TaqI TCGA 2 cut(s) 634, 704
TasI AATT 4 cut(s) 290, 324, 609, 731
TfiI GAWTC 3 cut(s) 64, 329, 422
Tru1I TTAA 2 cut(s) 287, 534
Tru9I TTAA 2 cut(s) 287, 534
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 281, 692
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 4 cut(s) 161, 632, 794, 844
TspGWI ACGGA 1 cut(s) 558
VpaK11BI GGWCC 2 cut(s) 589, 871
XagI CCTNNNNNAGG 1 cut(s) 435
XapI RAATTY 1 cut(s) 290
XceI RCATGY 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 634
XspI CTAG 1 cut(s) 297
ZraI GACGTC 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.