MD01G1096500.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000071
Physical Location & Seq
Reverse (-)
21111794 .. 21116039
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1096500.v1.1.491

Sequence Viewer

Length: 3180 bp
ATGACTGAAGAAAGTTCTGTGAATCATGATGAGGAAACCCGGCACAATCTCTCTCTAAACTTCTCAGACGTTGAGGTTAACATCAATCAACGTTTGTGTTCAGTTCTCTTGAACGAGTTCAATTATCTTCCTTGGTCCCGAGCTGTTTCACTAGCTCTCGGAGGCAAAGGGAAGTTAGGGTTTGTAAATGGAAGCGTGGAAGCTCCAGACAGTTCTTCCTCTACATACAATGCATGGCTCTGCAAAGATCAACTTGTCATGTCCTTGCTGCTCAACACCATGGAGAAACATGTTGCTGAAATTTTTAGCTACTCCAATTCCGCACATGATCTTTGGAAAGCTTTGCAGGATATGTATGGAAATCAAAACAACTATGCACGGGTCTTCCAATTAAAGAAGGACATTGCCAGTGCTCAACAAGAAGGGAAAGCATTTGTTCAACACCTTGGGAGCCTCAAAGCCATGTGGAATGAGTTGGATGTATATCTCCCTCATACCACAGATCCTACCATTCTCCTAAAACGAACTGAGGAAGACAAAATTTATCAGCTGTTAGGGAGTTTGAGCTCTGAGTATGAGGACCTAAGGAGTCACATCTTGATGAGTCAAGACCTGCCTACCTTCAACAATGTTTGTGCAACTATCCAACGAGAAGAAGCAAGGAAGAAGGTTATGAATGTTGATCACAATCCTAGATTAACAGAGACTCGGGCATATGCATCAAAGTACAAGAACTCAGAAGCCAAGGTATACAAGGGAAGAAACACACATCTAAAATGCAAATATTGCAATGGTGTTGGTCATGAGGAAGACAAGTGTTGGGAACTCCATCCTGAACTCAAGCCTAAGTTCAGCAAAGATGGAAGGATGATACCAAGGGGCACGGAGGAGGCTCACATGGTCAAGGCAATGAAGAGGGTAGTCACACAGCTATGTTGGGACAATTTTGCCGGATTTCTTGCTGGAAATGAGAAAGTTCCAAAGGGAGAAGCATCAGGTATACTCAAAGCCTTTACTACTGCCCTATTAACTAGTACTGAAATTGATTGTTGGATAATTGATTCAGGCGCCACAGATCATATGACAAACAAAGTTACAAATTTACATCATTTTAAAAAAATTTCAAGCCCCTCACATGTGTCTGTTGCAAATGGAAAAAATGTCTTAGTTTTGGGAAAAGGAGAAATAAACTTACTCTCTCAAAAAACACCTTCCACTGCCCTCTATGTACCAACCTTTCCCTTCCAACTTTTGTCAATTGGAAAGATCACCAACACCTTAAACTGCCGTGCTATTTTCTCACCAAATAAAGTAATGTTTCAGGATGTTCTCACCATGAAGATGATTGGTGAAGGGGTCTTCATAAATGGACTTTACTACCTGTGCAAGAATGCATGTTTCACTCAGGCTCTTCAAGCTCAATCGAAGTCCATAGATGAAAACCAACTTTGGCATAGGAGATTAGCTCACCCTTCTGAACTTGTCTTGTCAAAACTGTTTCCAAATTTTTGTAACCCCTATCCTACTTGTGATACTTGTCAATTTTCTAAGTTTACTAGGCTACCTTTGGGTAATTCAATGTCTAGGACAAGTAAACCTTTTGAAATGATACACACTGATGTATGGGGACCTGCAACTGAATCAATGGAAGGTTTTAAATATTTTGTTCTATTTGTTGATGATTTCTCTCGAACAAGTTTTCTATATCTTATGAAATCAAAAAGTGAAGTCCCTACCATTTTCAAAGACTTTCACATGCATGTTAAAACGCAATTTCAATCAAACATTAAGGTTTTAAGGTCAGATAATGGCACTGAATTTCTATCCAACAACATGCTTCAATACATAAGTTCTCAGGGTATCATACATCAAACCAGCTGTGTGGGGACTCCCCAACAAAATGGAGTAGCCGAGAGGAAAAATAGAGATCTCTTGGAAAAAAACTCGTGCTCTCATGATTCACATGCACGTTCCAAAGAAATTTTGGTCGTTTGCCATCCTTACTGCCACTTATCTCATCAATCGACTTCCAAGTCGTGTGCTAGGAATGCTTTCCTTCACGGCGAGCTGCAGGAAGAAGTGTATATGCAACCTCCTCCAGGCTATGATGGTATTAAAGGCAACATGGTTTGTAAATTGCACAAGGCCATATACGGATTGAAACAATCACCAAGAGCTTGGTATGCCAAGCTCAGTTCTGTTCTGGAAAAGGCTGGATTCATGCGAAGTAATGCTGATTCTTCGCTATTTGTAAGAACTGGCACCAGGGGGAAGTTGGTGGTCCTCGTCTATGTTGATGATCTTATCATCACTGGAGATAATACCGTGGAGATTGAGGCACTAAAACTCTCACTACATCAAGCTTTCGCTATCAAAGATTTGGGGAGGTTAAAGTATTTTTTGGGGATCGAAATGGCAACATCTTCAAAAGGACTGTTTCTACATCAACGGAAATATGTATTGGACCTCCTTCAAGAAGCAAAAATGCTTGACTGCAAGCCTGCTATCACTCCCGTTGATTGTAAACTGAAGCTCAGCATAGATGGAGAAGCCATGCATGATGTAAGCTACTATCAACGATTAGTAGGCAAGCTTATATACCTCACTATCACTCGTCCAGATATCACATATGCAGTGAGCTTGGCTAGTCAGTTCATGCACTCTCCCACTGTTGATCACCTTAACCTTGTTAAGAGAATCCTTCGTTATCTTAAGGGTTCAATTGGGCAAGGAATTACAATGCATAACAATCAGTCCACTGTCATTAGTGGCTACACAGATGCAGACTGGGCAGGTAATGCAATTGATAGGAAATCAATCACAGGCTATTGTACATTTGTTGGTGGAAACCTTGTCACATGGAAGAGTAAGAAGCAACAAGTAATTGCTCGTTCCAGCGCAGAGGCTGAGTATCGAGCCATGGCAGCCACTGCGTGTGAACTCATTTGGCTTAAAGGGCTTCTTTCAGACTTAGGGTTTCCTAGTTCTACTCCTATGACGCTTATGTGTGATAATCAGGCAGCCATGCACATTGCTGCCAATCCTGTGTTCCATGAAAGAACCAAACATATTGAAGTGGATTGTCATTTCATCAGAGCTCAAGTTCAAACGCAAATCATCCGGACCATGTTCACACGAAGCCATGATCAACTAGCAGATCTTTTTAAAATGCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1060

Amino Acids

119.44

Weight (kDa)

8.74

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 32 - 70 7.3e-13 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 53 - 224 3.9e-16 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 351 - 425 3.6e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 457 - 517 2.7e-09 GAG-pre-integrase domain
rve PF00665 531 - 631 3.4e-11 Integrase core domain
RVT_2 PF07727 683 - 839 2.3e-44 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2032
Acc36I ACCTGC 3 cut(s) 621, 1639, 2783
AccB1I GGYRCC 2 cut(s) 1067, 2261
AccI GTMKAC 2 cut(s) 750, 1000
AccIII TCCGGA 1 cut(s) 3120
AciI CCGC 1 cut(s) 321
AclI AACGTT 1 cut(s) 91
AclWI GGATC 2 cut(s) 497, 2414
AcsI RAATTY 7 cut(s) 300, 540, 1099, 1119, 1504, 1817, 1980
AcuI CTGAAG 2 cut(s) 27, 2549
AcyI GRCGYC 1 cut(s) 1068
AdeI CACNNNGTG 1 cut(s) 2934
AfaI GTAC 4 cut(s) 728, 1036, 1230, 2833
AfiI CCNNNNNNNGG 1 cut(s) 2099
AflII CTTAAG 1 cut(s) 2711
AflIII ACRYGT 2 cut(s) 289, 1135
AhlI ACTAGT 1 cut(s) 1031
AjnI CCWGG 2 cut(s) 2098, 2264
AjuI GAANNNNNNNTTGG 4 cut(s) 2444, 2476, 3032, 3064
Alw21I GWGCWC 4 cut(s) 415, 569, 1952, 3100
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 2 cut(s) 497, 2414
AlwNI CAGNNNCTG 2 cut(s) 2906, 2930
Ama87I CYCGRG 2 cut(s) 138, 708
Aor13HI TCCGGA 1 cut(s) 3120
AoxI GGCC 1 cut(s) 2145
ApeKI GCWGC 5 cut(s) 268, 2068, 2924, 3020, 3035
ApoI RAATTY 7 cut(s) 300, 540, 1099, 1119, 1504, 1817, 1980
Asp700I GAANNNNTTC 2 cut(s) 116, 2051
AspLEI GCGC 2 cut(s) 1070, 2900
AspS9I GGNCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 7 cut(s) 1261, 1293, 1324, 1361, 1460, 2160, 2669
AvaI CYCGRG 2 cut(s) 138, 708
AvaII GGWCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
AxyI CCTNAGG 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 884
BanI GGYRCC 2 cut(s) 1067, 2261
BanII GRGCYC 2 cut(s) 569, 3100
BauI CACGAG 1 cut(s) 1945
BbsI GAAGAC 4 cut(s) 376, 540, 816, 1351
Bbv12I GWGCWC 4 cut(s) 415, 569, 1952, 3100
BbvI GCAGC 5 cut(s) 255, 2055, 2936, 3022, 3032
BccI CCATC 5 cut(s) 837, 854, 2004, 2102, 2537
BceAI ACGGC 2 cut(s) 1272, 2077
BciT130I CCWGG 2 cut(s) 2100, 2266
BclI TGATCA 3 cut(s) 682, 2674, 3145
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 698
BcuI ACTAGT 1 cut(s) 1031
BfaI CTAG 9 cut(s) 152, 693, 1032, 1557, 1584, 2043, 2646, 2982, 3152
BfmI CTRYAG 1 cut(s) 2069
BfoI RGCGCY 1 cut(s) 1071
BfrI CTTAAG 1 cut(s) 2711
BfuAI ACCTGC 3 cut(s) 621, 1639, 2783
BglII AGATCT 2 cut(s) 1927, 3157
BisI GCNGC 5 cut(s) 269, 2069, 2925, 3021, 3036
BlpI GCTNAGC 1 cut(s) 2534
BlsI GCNGC 5 cut(s) 270, 2070, 2926, 3022, 3037
BmcAI AGTACT 1 cut(s) 1036
Bme1390I CCNGG 3 cut(s) 40, 2100, 2266
Bme18I GGWCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
BmeT110I CYCGRG 2 cut(s) 138, 708
BmgT120I GGNCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
BmiI GGNNCC 5 cut(s) 137, 452, 1069, 1629, 2263
BmrFI CCNGG 3 cut(s) 40, 2100, 2266
BmrI ACTGGG 1 cut(s) 2797
BmsI GCATC 3 cut(s) 728, 1001, 2770
BmuI ACTGGG 1 cut(s) 2797
BpiI GAAGAC 4 cut(s) 376, 540, 816, 1351
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
BpmI CTGGAG 3 cut(s) 189, 2082, 2334
Bpu1102I GCTNAGC 1 cut(s) 2534
BpuEI CTTGAG 2 cut(s) 824, 3084
BpuMI CCSGG 1 cut(s) 40
BsaBI GATNNNNATC 2 cut(s) 483, 687
BsaHI GRCGYC 1 cut(s) 1068
BsaJI CCNNGG 8 cut(s) 131, 279, 445, 744, 875, 2265, 2325, 2919
BsaWI WCCGGW 1 cut(s) 3120
Bsc4I CCNNNNNNNGG 1 cut(s) 2099
Bse1I ACTGG 4 cut(s) 408, 2263, 2317, 2792
Bse21I CCTNAGG 1 cut(s) 584
Bse3DI GCAATG 4 cut(s) 402, 796, 915, 3030
Bse8I GATNNNNATC 2 cut(s) 483, 687
BseAI TCCGGA 1 cut(s) 3120
BseBI CCWGG 2 cut(s) 2100, 2266
BseDI CCNNGG 8 cut(s) 131, 279, 445, 744, 875, 2265, 2325, 2919
BseGI GGATG 6 cut(s) 484, 829, 873, 1330, 1996, 3117
BseJI GATNNNNATC 2 cut(s) 483, 687
BseLI CCNNNNNNNGG 1 cut(s) 2099
BseMI GCAATG 4 cut(s) 402, 796, 915, 3030
BseMII CTCAG 9 cut(s) 78, 519, 561, 750, 1418, 1868, 2206, 2548, 2898
BseNI ACTGG 4 cut(s) 408, 2263, 2317, 2792
BseRI GAGGAG 2 cut(s) 902, 2085
BseSI GKGCMC 1 cut(s) 884
BseXI GCAGC 5 cut(s) 255, 2055, 2936, 3022, 3032
BshFI GGCC 1 cut(s) 2147
BshNI GGYRCC 2 cut(s) 1067, 2261
BsiHKAI GWGCWC 4 cut(s) 415, 569, 1952, 3100
BsiHKCI CYCGRG 2 cut(s) 138, 708
BsiSI CCGG 3 cut(s) 40, 951, 3121
BslFI GGGAC 5 cut(s) 121, 953, 1641, 1715, 1900
BslI CCNNNNNNNGG 1 cut(s) 2099
BsmAI GTCTC 1 cut(s) 698
BsmFI GGGAC 5 cut(s) 121, 953, 1641, 1715, 1900
BsmI GAATGC 2 cut(s) 1396, 2053
BsnI GGCC 1 cut(s) 2147
BsoBI CYCGRG 2 cut(s) 138, 708
Bsp1286I GDGCHC 5 cut(s) 415, 569, 884, 1952, 3100
Bsp13I TCCGGA 1 cut(s) 3120
Bsp1407I TGTACA 1 cut(s) 2831
Bsp1720I GCTNAGC 1 cut(s) 2534
Bsp19I CCATGG 2 cut(s) 279, 2919
BspACI CCGC 1 cut(s) 321
BspANI GGCC 1 cut(s) 2147
BspCNI CTCAG 9 cut(s) 77, 520, 562, 749, 1417, 1867, 2205, 2547, 2899
BspEI TCCGGA 1 cut(s) 3120
BspHI TCATGA 3 cut(s) 25, 802, 1954
BspLI GGNNCC 5 cut(s) 137, 452, 1069, 1629, 2263
BspMAI CTGCAG 1 cut(s) 2073
BspMI ACCTGC 3 cut(s) 621, 1639, 2783
BspPI GGATC 2 cut(s) 497, 2414
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 2 cut(s) 1067, 2261
BspTI CTTAAG 1 cut(s) 2711
BsrDI GCAATG 4 cut(s) 402, 796, 915, 3030
BsrGI TGTACA 1 cut(s) 2831
BsrI ACTGG 4 cut(s) 408, 2263, 2317, 2792
BssECI CCNNGG 8 cut(s) 131, 279, 445, 744, 875, 2265, 2325, 2919
BssNAI GTATAC 2 cut(s) 751, 1001
BssNI GRCGYC 1 cut(s) 1068
BssSI CACGAG 1 cut(s) 1945
BssT1I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 2919
Bst1107I GTATAC 2 cut(s) 751, 1001
Bst2BI CACGAG 1 cut(s) 1945
Bst2UI CCWGG 2 cut(s) 2100, 2266
Bst4CI ACNGT 6 cut(s) 212, 1497, 2326, 2436, 2671, 2761
Bst6I CTCTTC 3 cut(s) 908, 1416, 2858
BstACI GRCGYC 1 cut(s) 1068
BstAFI CTTAAG 1 cut(s) 2711
BstAPI GCANNNNNTGC 3 cut(s) 786, 2798, 2930
BstAUI TGTACA 1 cut(s) 2831
BstC8I GCNNGC 5 cut(s) 2066, 2498, 2502, 2591, 3174
BstDSI CCRYGG 3 cut(s) 279, 2325, 2919
BstENI CCTNNNNNAGG 1 cut(s) 2097
BstF5I GGATG 6 cut(s) 484, 829, 873, 1330, 1996, 3117
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 2 cut(s) 1070, 2900
BstMAI GTCTC 1 cut(s) 698
BstMWI GCNNNNNNNGC 9 cut(s) 786, 1415, 2048, 2183, 2789, 2798, 2924, 2930, 2956
BstNI CCWGG 2 cut(s) 2100, 2266
BstNSI RCATGY 8 cut(s) 293, 1139, 1398, 1759, 1763, 1837, 1967, 3176
BstSCI CCNGG 3 cut(s) 38, 2098, 2264
BstSFI CTRYAG 1 cut(s) 2069
BstSLI GKGCMC 1 cut(s) 884
BstV1I GCAGC 5 cut(s) 255, 2055, 2936, 3022, 3032
BstV2I GAAGAC 4 cut(s) 376, 540, 816, 1351
BstX2I RGATCY 3 cut(s) 502, 1927, 3157
BstXI CCANNNNNNTGG 3 cut(s) 1882, 1901, 2178
BstYI RGATCY 3 cut(s) 502, 1927, 3157
BstZ17I GTATAC 2 cut(s) 751, 1001
Bsu36I CCTNAGG 1 cut(s) 584
BsuRI GGCC 1 cut(s) 2147
BtgI CCRYGG 3 cut(s) 279, 2325, 2919
BtsCI GGATG 6 cut(s) 484, 829, 873, 1330, 1996, 3117
BtsI GCAGTG 3 cut(s) 1215, 2640, 2928
BtsIMutI CAGTG 9 cut(s) 415, 1215, 1614, 1812, 2310, 2640, 2667, 2757, 2928
BveI ACCTGC 3 cut(s) 621, 1639, 2783
Cac8I GCNNGC 5 cut(s) 2066, 2498, 2502, 2591, 3174
CaiI CAGNNNCTG 2 cut(s) 2906, 2930
CciI TCATGA 3 cut(s) 25, 802, 1954
CfoI GCGC 2 cut(s) 1070, 2900
Cfr13I GGNCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
CseI GACGC 1 cut(s) 3007
Csp6I GTAC 4 cut(s) 727, 1035, 1229, 2832
CviQI GTAC 4 cut(s) 727, 1035, 1229, 2832
DinI GGCGCC 1 cut(s) 1069
DraI TTTAAA 3 cut(s) 1114, 1657, 3166
DraIII CACNNNGTG 1 cut(s) 2934
DrdI GACNNNNNNGTC 1 cut(s) 2032
DseDI GACNNNNNNGTC 1 cut(s) 2032
Eam1104I CTCTTC 3 cut(s) 908, 1416, 2858
EarI CTCTTC 3 cut(s) 908, 1416, 2858
Ecl136II GAGCTC 2 cut(s) 567, 3098
Eco130I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 2919
Eco24I GRGCYC 2 cut(s) 569, 3100
Eco32I GATATC 1 cut(s) 2623
Eco47I GGWCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
Eco53kI GAGCTC 2 cut(s) 567, 3098
Eco57I CTGAAG 2 cut(s) 27, 2549
Eco81I CCTNAGG 1 cut(s) 584
Eco88I CYCGRG 2 cut(s) 138, 708
EcoICRI GAGCTC 2 cut(s) 567, 3098
EcoNI CCTNNNNNAGG 1 cut(s) 2097
EcoO109I RGGNCCY 2 cut(s) 580, 1628
EcoRII CCWGG 2 cut(s) 2098, 2264
EcoRV GATATC 1 cut(s) 2623
EcoT14I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 2919
EcoT22I ATGCAT 8 cut(s) 235, 721, 1396, 1761, 2559, 2745, 3174, 3178
EcoT38I GRGCYC 2 cut(s) 569, 3100
EgeI GGCGCC 1 cut(s) 1069
EheI GGCGCC 1 cut(s) 1069
ErhI CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 2919
FalI AAGNNNNNCTT 6 cut(s) 237, 269, 1582, 1614, 2946, 2978
FaqI GGGAC 5 cut(s) 121, 953, 1641, 1715, 1900
FauNDI CATATG 3 cut(s) 715, 1080, 2629
FbaI TGATCA 3 cut(s) 682, 2674, 3145
FblI GTMKAC 2 cut(s) 750, 1000
Fnu4HI GCNGC 5 cut(s) 269, 2069, 2925, 3021, 3036
FokI GGATG 6 cut(s) 491, 816, 880, 1337, 1983, 3104
FriOI GRGCYC 2 cut(s) 569, 3100
Fsp4HI GCNGC 5 cut(s) 269, 2069, 2925, 3021, 3036
FspBI CTAG 9 cut(s) 152, 693, 1032, 1557, 1584, 2043, 2646, 2982, 3152
GlaI GCGC 2 cut(s) 1069, 2899
GluI GCNGC 5 cut(s) 269, 2069, 2925, 3021, 3036
GsuI CTGGAG 3 cut(s) 189, 2082, 2334
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 1 cut(s) 2147
HapII CCGG 3 cut(s) 40, 951, 3121
HgaI GACGC 1 cut(s) 3007
HhaI GCGC 2 cut(s) 1070, 2900
Hin1I GRCGYC 1 cut(s) 1068
Hin6I GCGC 2 cut(s) 1068, 2898
HinP1I GCGC 2 cut(s) 1068, 2898
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 3 cut(s) 339, 2361, 2591
HpaI GTTAAC 1 cut(s) 79
HpaII CCGG 3 cut(s) 40, 951, 3121
HphI GGTGA 7 cut(s) 1261, 1293, 1324, 1361, 1460, 2160, 2669
Hpy166II GTNNAC 9 cut(s) 79, 751, 1001, 1554, 1595, 2525, 2757, 2939, 3132
Hpy188I TCNGA 8 cut(s) 67, 161, 571, 739, 1477, 1804, 2968, 3095
Hpy8I GTNNAC 9 cut(s) 79, 751, 1001, 1554, 1595, 2525, 2757, 2939, 3132
HpyCH4III ACNGT 6 cut(s) 212, 1497, 2326, 2436, 2671, 2761
HpyCH4IV ACGT 3 cut(s) 69, 91, 1969
HpyF10VI GCNNNNNNNGC 9 cut(s) 786, 1415, 2048, 2183, 2789, 2798, 2924, 2930, 2956
HpySE526I ACGT 3 cut(s) 69, 91, 1969
Hsp92I GRCGYC 1 cut(s) 1068
HspAI GCGC 2 cut(s) 1068, 2898
KasI GGCGCC 1 cut(s) 1067
Kpn2I TCCGGA 1 cut(s) 3120
Ksp22I TGATCA 3 cut(s) 682, 2674, 3145
KspAI GTTAAC 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 2 cut(s) 208, 450
Lsp1109I GCAGC 5 cut(s) 255, 2055, 2936, 3022, 3032
LweI GCATC 3 cut(s) 728, 1001, 2770
MaeI CTAG 9 cut(s) 152, 693, 1032, 1557, 1584, 2043, 2646, 2982, 3152
MaeII ACGT 3 cut(s) 69, 91, 1969
MaeIII GTNAC 5 cut(s) 590, 922, 1093, 1511, 2854
MfeI CAATTG 3 cut(s) 1257, 2721, 2802
MflI RGATCY 3 cut(s) 502, 1927, 3157
MhlI GDGCHC 5 cut(s) 415, 569, 884, 1952, 3100
Mly113I GGCGCC 1 cut(s) 1068
MlyI GAGTC 4 cut(s) 598, 613, 700, 1882
MmeI TCCRAC 5 cut(s) 456, 670, 1031, 1270, 1851
Mph1103I ATGCAT 8 cut(s) 235, 721, 1396, 1761, 2559, 2745, 3174, 3178
MroI TCCGGA 1 cut(s) 3120
MroXI GAANNNNTTC 2 cut(s) 116, 2051
MslI CAYNNNNRTG 5 cut(s) 599, 931, 1340, 1617, 1758
MspA1I CMGCKG 2 cut(s) 550, 1878
MspCI CTTAAG 1 cut(s) 2711
MspI CCGG 3 cut(s) 40, 951, 3121
MspR9I CCNGG 3 cut(s) 40, 2100, 2266
MunI CAATTG 3 cut(s) 1257, 2721, 2802
Mva1269I GAATGC 2 cut(s) 1396, 2053
MvaI CCWGG 2 cut(s) 2100, 2266
MwoI GCNNNNNNNGC 9 cut(s) 786, 1415, 2048, 2183, 2789, 2798, 2924, 2930, 2956
NarI GGCGCC 1 cut(s) 1068
NciI CCSGG 1 cut(s) 40
NcoI CCATGG 2 cut(s) 279, 2919
NdeI CATATG 3 cut(s) 715, 1080, 2629
NlaIV GGNNCC 5 cut(s) 137, 452, 1069, 1629, 2263
NmeAIII GCCGAG 1 cut(s) 1936
NmuCI GTSAC 3 cut(s) 590, 922, 2854
NsiI ATGCAT 8 cut(s) 235, 721, 1396, 1761, 2559, 2745, 3174, 3178
NspI RCATGY 8 cut(s) 293, 1139, 1398, 1759, 1763, 1837, 1967, 3176
PaeI GCATGC 1 cut(s) 3176
PagI TCATGA 3 cut(s) 25, 802, 1954
PciI ACATGT 2 cut(s) 289, 1135
PciSI GCTCTTC 1 cut(s) 1416
PctI GAATGC 2 cut(s) 1396, 2053
PdmI GAANNNNTTC 2 cut(s) 116, 2051
PfeI GAWTC 7 cut(s) 22, 1061, 1640, 1958, 2217, 2237, 2697
PkrI GCNGC 5 cut(s) 270, 2070, 2926, 3022, 3037
PleI GAGTC 4 cut(s) 597, 612, 700, 1882
PluTI GGCGCC 1 cut(s) 1071
PpsI GAGTC 4 cut(s) 597, 612, 700, 1882
PpuMI RGGWCCY 2 cut(s) 580, 1628
PscI ACATGT 2 cut(s) 289, 1135
Psp124BI GAGCTC 2 cut(s) 569, 3100
Psp1406I AACGTT 1 cut(s) 91
Psp5II RGGWCCY 2 cut(s) 580, 1628
Psp6I CCWGG 2 cut(s) 2098, 2264
PspGI CCWGG 2 cut(s) 2098, 2264
PspN4I GGNNCC 5 cut(s) 137, 452, 1069, 1629, 2263
PspPI GGNCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
PspPPI RGGWCCY 2 cut(s) 580, 1628
PstI CTGCAG 1 cut(s) 2073
PstNI CAGNNNCTG 2 cut(s) 2906, 2930
PsuI RGATCY 3 cut(s) 502, 1927, 3157
PvuII CAGCTG 2 cut(s) 550, 1878
RsaI GTAC 4 cut(s) 728, 1036, 1230, 2833
RsaNI GTAC 4 cut(s) 727, 1035, 1229, 2832
RseI CAYNNNNRTG 5 cut(s) 599, 931, 1340, 1617, 1758
SacI GAGCTC 2 cut(s) 569, 3100
SapI GCTCTTC 1 cut(s) 1416
SatI GCNGC 5 cut(s) 269, 2069, 2925, 3021, 3036
Sau96I GGNCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
ScaI AGTACT 1 cut(s) 1036
SchI GAGTC 4 cut(s) 598, 613, 700, 1882
ScrFI CCNGG 3 cut(s) 40, 2100, 2266
SduI GDGCHC 5 cut(s) 415, 569, 884, 1952, 3100
SfaNI GCATC 3 cut(s) 728, 1001, 2770
SfcI CTRYAG 1 cut(s) 2069
SfoI GGCGCC 1 cut(s) 1069
SinI GGWCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
SmiMI CAYNNNNRTG 5 cut(s) 599, 931, 1340, 1617, 1758
SmlI CTYRAG 3 cut(s) 839, 2711, 3099
SmoI CTYRAG 3 cut(s) 839, 2711, 3099
SpeI ACTAGT 1 cut(s) 1031
SphI GCATGC 1 cut(s) 3176
SsiI CCGC 1 cut(s) 321
SspDI GGCGCC 1 cut(s) 1067
SspI AATATT 2 cut(s) 785, 1661
SspMI CTAG 9 cut(s) 152, 693, 1032, 1557, 1584, 2043, 2646, 2982, 3152
SstI GAGCTC 2 cut(s) 569, 3100
StyD4I CCNGG 3 cut(s) 38, 2098, 2264
StyI CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 2919
TaaI ACNGT 6 cut(s) 212, 1497, 2326, 2436, 2671, 2761
TaiI ACGT 3 cut(s) 72, 94, 1972
TaqI TCGA 5 cut(s) 1424, 1690, 2024, 2409, 2914
TatI WGTACW 3 cut(s) 726, 1034, 2831
TfiI GAWTC 7 cut(s) 22, 1061, 1640, 1958, 2217, 2237, 2697
TscAI CASTG 9 cut(s) 415, 1222, 1621, 1819, 2317, 2640, 2674, 2764, 2935
TseFI GTSAC 3 cut(s) 590, 922, 2854
TseI GCWGC 5 cut(s) 268, 2068, 2924, 3020, 3035
Tsp45I GTSAC 3 cut(s) 590, 922, 2854
TspGWI ACGGA 3 cut(s) 899, 2169, 2464
TspRI CASTG 9 cut(s) 415, 1222, 1621, 1819, 2317, 2640, 2674, 2764, 2935
Vha464I CTTAAG 1 cut(s) 2711
VpaK11BI GGWCC 6 cut(s) 135, 580, 1628, 2281, 2464, 3123
XagI CCTNNNNNAGG 1 cut(s) 2097
XapI RAATTY 7 cut(s) 300, 540, 1099, 1119, 1504, 1817, 1980
XceI RCATGY 8 cut(s) 293, 1139, 1398, 1759, 1763, 1837, 1967, 3176
XcmI CCANNNNNNNNNTGG 2 cut(s) 1981, 2272
XmiI GTMKAC 2 cut(s) 750, 1000
XmnI GAANNNNTTC 2 cut(s) 116, 2051
XspI CTAG 9 cut(s) 152, 693, 1032, 1557, 1584, 2043, 2646, 2982, 3152
ZrmI AGTACT 1 cut(s) 1036
Zsp2I ATGCAT 8 cut(s) 235, 721, 1396, 1761, 2559, 2745, 3174, 3178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.