FvH4_2g24231

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000071
Physical Location & Seq
Reverse (-)
19796091 .. 19798646
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24231.t1

Sequence Viewer

Length: 2556 bp
ATGGTGTCCACACAGTATCAACAGGATGTTCGAGTTTTCCAGTCTGATAATGGCGGAGAATATATTAATGGTCCTATGCAAGAGTTTATGAAGTTACATGGAATTCGACATCAGACTTCAAATACTTATACTCCTCAACAAAATGGATTGGCAGAGAAGAAAAATCGGCAGTTGCTTGAAGTTGTCCGTGCCTCTTTGTTTGGCATGAATGTACCTGTAGAATATTGGGGAGAAGCTGTGACATCTGCTGCCTACCTCATTAATCGTACCCCTTCTCGGGTTATTGAATGTGAAAACCCTCTACAACAGCTTCAGAATCTTCTCTCGTTTCCTTCGTTGCCTAACTTGGAGCCTCGTGTATTTGGATGTACAGTGTATGTTCATATTCTAAAACAACAACGGAGTAAGCTTGATCCCCGTGCAAGGAAGTGTATCTTTGTGGGTTATGCAGATTTTCAGAAAGGATATAGATGTTATGATCCTCTTACAGACACTATGCATGTATCCCTTGATGTTTCTTTTCGTGAATCAGAGCCTTACTACTCAGGGGGAGTATCAGAGTCTTCCCTTCAGGGGGAGAGAGCATATGAAGGGAATTCCCAAAATTTATTTGAATTTGAAGAATTTGAAGAATTAGAAGAATTGGAGTCGAGATTTGGAGCAGGAGGAAACAAAAATCATGAAAACGACGTTGTACAGCAGCATACCAACATTGACAAAAACAGTGGGCCTCGCGTCGAAGTGGCTGATGGCGTGCCACATGTCGAACAACAGCGGATCTCCTCTGATGCAATAGAATGTGAGCCGAGTGAGTCGAGCCATAAGCCAAACGGAGCGGAAGCTGAGCCACAATTTCAGCTTGGGTCACGTGTCTACGTTGATCCAGTTTTGGGGAGTGAAGCTGTGCCACATGTTGTGCACAGAGAGGTTGGCATGACTAACGAAGAAGAGATTGTCGGACTGGAAGAGGCAGGCACGCAGATAGGGTCACGTGTCGCGCATGGATCGGCTGATGCAAACCAACAAGAACCACAATTTTCAACTGATTCGACCACTTGCTCTTCGGATATTTTTCCCTTTTCTACACCATCAACCGAAGAATCCGCTGCAAATGTCCCTTCTCAGGTATCTTTGAATTCAAACCAATTATCTGGGATAGATAATATTGTAACTAGGAAGTCTCAGAGGGTTACCAAGGGTATTCCAAAGAAACAATATGAACCAGACATCAAAGCCAAAGCTAAATATCCTATAGCTAATTCTATGTCTAACCATAGGTTGGCTGGGTCACATGCTCTTATCATTAATCAATTATCCAGTGTTTCTATTCCTAGTACTGTACAGGATGCAATGGCGGATCCTAAGTGGACTCAAGCGATGAATGAAGAACTAGAAGCCTTGCAAAAGAACTCGACCTGGGATGTTATTCATAAACCTATTGGAAAGAAGACTGTTGGATGTCGTTGGGTATTTACGGTTAAACTTAAAGCCGATGGGAGCATTGACAGGTATAAAGCCAGATTAGTTGCCAAAGGGTACACCCAGCGTTATTGGATTGATTATGAAGAAACGTTTGCGCCTGTAGCAAAAATCAACACTGTTCGAATCTTGATTTCACTTGCGGCAACTAAAGATTGGCCTCTACGACAGTTTGATGTGAAGAATGCTTTCCTCAATAGAAACTTGGAAGAAGAGGTGTATATGGATATGCCCCCAGGAGTCAAATGTAGACCTTGCGATGTTGGAAAGGTTTGTAAGTTGAAGAAGTCTCTGTATGGTTTGAAGCAGTCTCCTAGAGCTTGGTTTGGAAGATTCTCCAAATCAATGAAGACATTTGGTTATCGACAAAGCAACTCAGATCACACTTTGTTCATCAAACGTAGTCAGGGGAAGATCACCGCTCTGATTGTGTATGTTGATGATATGATTGTTACAGGAGACGACCCAAAAGAGATGGAGGCTCTACAGAAGTACCTTTCAAAAGACTTTGAGATGAAGGACCTTCGACAGTTGAAGTATTTTCTCGGAATTGAAGTTGCAAGGTCAAAGACGGGGATTTCTTTATCCCAACGAAAGTATGTACTTGACTTGTTAGCTGAGACCGGAATGCTTGACTGCAAACCTGTTGAGACACCAATTGAGATGAATCACAACCTTGCCATCTATCCGAATCAAGTTCCAACTGATAAAAGAAGGTACCAACGTCTAGTAGGGAGACTTATTTATCTTTCTCATACTAGACCTGATATTGCTTATCCTGTGAGTGTGGTTAGTCAATTTATGTATTGTTCTAGCGAAGAGCATATGGATGCAGTGTATCGTATTCTAAAGTATCTTAAGACAGCACCTGGAAAAGGTTTGTTATTTGAAAAGAAGGGTGAATCAGAAGTTGTAGGGTACACAGATGCTGATTGGGCTGGTGATAAGACTGACAGACGATCTACATCTGGGTATTTTACATTTGTTGGAGGAAACCTTGTAACTTGGCGTAGTAAGAAACAAAAAGTCGTTGCAAGATCAAGTGCTGAAGCAGAGTTTCGAGGTATGGCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC RVT_2 TIR

Protein Analysis

852

Amino Acids

96.0

Weight (kDa)

7.03

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 123 - 180 3.4e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 470 - 714 5e-71 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2196
AccB1I GGYRCC 1 cut(s) 2196
AccB7I CCANNNNNTGG 1 cut(s) 1279
AccBSI CCGCTC 2 cut(s) 836, 1901
AccI GTMKAC 2 cut(s) 873, 1729
AccII CGCG 2 cut(s) 735, 998
AciI CCGC 7 cut(s) 54, 775, 836, 1104, 1355, 1622, 1899
AclI AACGTT 1 cut(s) 1571
AclWI GGATC 7 cut(s) 407, 473, 785, 875, 1012, 1352, 1365
AcsI RAATTY 6 cut(s) 102, 595, 604, 614, 623, 1135
AcuI CTGAAG 3 cut(s) 296, 554, 2547
AcvI CACGTG 2 cut(s) 869, 992
AfiI CCNNNNNNNGG 9 cut(s) 276, 277, 423, 573, 574, 890, 1123, 1279, 2354
AflII CTTAAG 1 cut(s) 2336
AflIII ACRYGT 4 cut(s) 760, 868, 910, 991
AjnI CCWGG 3 cut(s) 1415, 1714, 2347
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 7 cut(s) 1185, 1773, 1794, 1932, 2093, 2123, 2209
Alw44I GTGCAC 1 cut(s) 917
AlwI GGATC 7 cut(s) 407, 473, 785, 875, 1012, 1352, 1365
AlwNI CAGNNNCTG 2 cut(s) 2348, 2408
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 2 cut(s) 728, 1637
ApaLI GTGCAC 1 cut(s) 917
ApeKI GCWGC 3 cut(s) 248, 700, 1106
ApoI RAATTY 6 cut(s) 102, 595, 604, 614, 623, 1135
AseI ATTAAT 3 cut(s) 66, 261, 1305
Asp700I GAANNNNTTC 1 cut(s) 1667
Asp718I GGTACC 1 cut(s) 2196
AspLEI GCGC 2 cut(s) 1000, 1579
AspS9I GGNCC 3 cut(s) 71, 728, 1999
AsuHPI GGTGA 3 cut(s) 1888, 2390, 2432
AsuII TTCGAA 1 cut(s) 1603
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 2 cut(s) 71, 1999
BaeGI GKGCMC 1 cut(s) 921
BaeI ACNNNNGTAYC 4 cut(s) 1955, 1988, 2188, 2221
BamHI GGATCC 1 cut(s) 1357
BanI GGYRCC 1 cut(s) 2196
BauI CACGAG 1 cut(s) 354
BbrPI CACGTG 2 cut(s) 869, 992
BbsI GAAGAC 3 cut(s) 555, 1454, 1835
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 3 cut(s) 235, 712, 1093
BccI CCATC 5 cut(s) 743, 1096, 1487, 1948, 2168
BciT130I CCWGG 3 cut(s) 1417, 1716, 2349
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 7 cut(s) 1185, 1773, 1794, 1932, 2093, 2123, 2209
BfaI CTAG 7 cut(s) 1173, 1332, 1391, 1794, 2207, 2238, 2292
BfmI CTRYAG 4 cut(s) 216, 1251, 1581, 1964
BfrI CTTAAG 1 cut(s) 2336
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 4 cut(s) 249, 701, 1107, 1623
BlpI GCTNAGC 1 cut(s) 843
BlsI GCNGC 4 cut(s) 250, 702, 1108, 1624
BmcAI AGTACT 1 cut(s) 1336
Bme1390I CCNGG 3 cut(s) 1417, 1716, 2349
Bme18I GGWCC 2 cut(s) 71, 1999
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 3 cut(s) 71, 728, 1999
BmiI GGNNCC 3 cut(s) 351, 1359, 2198
BmrFI CCNGG 3 cut(s) 1417, 1716, 2349
BmsI GCATC 5 cut(s) 778, 1003, 1336, 2299, 2395
BpiI GAAGAC 3 cut(s) 555, 1454, 1835
Bpu1102I GCTNAGC 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 1603
BpuEI CTTGAG 1 cut(s) 1356
BsaAI YACGTR 2 cut(s) 869, 992
BsaBI GATNNNNATC 1 cut(s) 2443
BsaI GGTCTC 1 cut(s) 2093
BsaJI CCNNGG 3 cut(s) 1194, 1416, 1714
BsaWI WCCGGW 1 cut(s) 2102
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 222, 252
Bsc4I CCNNNNNNNGG 9 cut(s) 276, 277, 423, 573, 574, 890, 1123, 1279, 2354
Bse1I ACTGG 4 cut(s) 40, 884, 966, 1317
Bse3DI GCAATG 1 cut(s) 1356
Bse8I GATNNNNATC 1 cut(s) 2443
BseBI CCWGG 3 cut(s) 1417, 1716, 2349
BseDI CCNNGG 3 cut(s) 1194, 1416, 1714
BseGI GGATG 6 cut(s) 31, 371, 1351, 1426, 1463, 2314
BseJI GATNNNNATC 1 cut(s) 2443
BseLI CCNNNNNNNGG 9 cut(s) 276, 277, 423, 573, 574, 890, 1123, 1279, 2354
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 6 cut(s) 558, 834, 1136, 1196, 1869, 2088
BseNI ACTGG 4 cut(s) 40, 884, 966, 1317
BseRI GAGGAG 2 cut(s) 123, 772
BseSI GKGCMC 1 cut(s) 921
BseXI GCAGC 3 cut(s) 235, 712, 1093
BseYI CCCAGC 2 cut(s) 1283, 1542
Bsh1236I CGCG 2 cut(s) 735, 998
BshFI GGCC 2 cut(s) 730, 1639
BshNI GGYRCC 1 cut(s) 2196
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 1 cut(s) 2103
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 9 cut(s) 276, 277, 423, 573, 574, 890, 1123, 1279, 2354
BsmAI GTCTC 7 cut(s) 1185, 1773, 1794, 1932, 2093, 2123, 2209
BsmBI CGTCTC 1 cut(s) 1932
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 2 cut(s) 1669, 2112
BsnI GGCC 2 cut(s) 730, 1639
Bso31I GGTCTC 1 cut(s) 2093
BsoBI CYCGRG 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 1603
Bsp1286I GDGCHC 1 cut(s) 921
Bsp1407I TGTACA 3 cut(s) 368, 694, 1339
Bsp1720I GCTNAGC 1 cut(s) 843
BspACI CCGC 7 cut(s) 54, 775, 836, 1104, 1355, 1622, 1899
BspANI GGCC 2 cut(s) 730, 1639
BspCNI CTCAG 6 cut(s) 557, 835, 1135, 1195, 1868, 2089
BspFNI CGCG 2 cut(s) 735, 998
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 3 cut(s) 351, 1359, 2198
BspPI GGATC 7 cut(s) 407, 473, 785, 875, 1012, 1352, 1365
BspQI GCTCTTC 2 cut(s) 1066, 2292
BspT104I TTCGAA 1 cut(s) 1603
BspT107I GGYRCC 1 cut(s) 2196
BspTI CTTAAG 1 cut(s) 2336
BspTNI GGTCTC 1 cut(s) 2093
BsrBI CCGCTC 2 cut(s) 836, 1901
BsrDI GCAATG 1 cut(s) 1356
BsrGI TGTACA 3 cut(s) 368, 694, 1339
BsrI ACTGG 4 cut(s) 40, 884, 966, 1317
BssECI CCNNGG 3 cut(s) 1194, 1416, 1714
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 1194
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 1417, 1716, 2349
Bst4CI ACNGT 9 cut(s) 15, 373, 725, 1339, 1453, 1477, 1600, 1650, 2010
Bst6I CTCTTC 5 cut(s) 942, 960, 1066, 1686, 2292
BstAFI CTTAAG 1 cut(s) 2336
BstAUI TGTACA 3 cut(s) 368, 694, 1339
BstBAI YACGTR 2 cut(s) 869, 992
BstBI TTCGAA 1 cut(s) 1603
BstC8I GCNNGC 3 cut(s) 755, 973, 977
BstDEI CTNAG 7 cut(s) 544, 843, 1122, 1182, 1362, 1855, 2097
BstEII GGTNACC 1 cut(s) 1189
BstENI CCTNNNNNAGG 1 cut(s) 2352
BstF5I GGATG 6 cut(s) 31, 371, 1351, 1426, 1463, 2314
BstFNI CGCG 2 cut(s) 735, 998
BstHHI GCGC 2 cut(s) 1000, 1579
BstMAI GTCTC 7 cut(s) 1185, 1773, 1794, 1932, 2093, 2123, 2209
BstMWI GCNNNNNNNGC 2 cut(s) 1583, 2414
BstNI CCWGG 3 cut(s) 1417, 1716, 2349
BstNSI RCATGY 4 cut(s) 503, 764, 914, 1295
BstPI GGTNACC 1 cut(s) 1189
BstSCI CCNGG 3 cut(s) 1415, 1714, 2347
BstSFI CTRYAG 4 cut(s) 216, 1251, 1581, 1964
BstSLI GKGCMC 1 cut(s) 921
BstUI CGCG 2 cut(s) 735, 998
BstV1I GCAGC 3 cut(s) 235, 712, 1093
BstV2I GAAGAC 3 cut(s) 555, 1454, 1835
BstX2I RGATCY 2 cut(s) 777, 1357
BstXI CCANNNNNNTGG 1 cut(s) 1151
BstYI RGATCY 2 cut(s) 777, 1357
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 730, 1639
BtgZI GCGATG 2 cut(s) 1391, 1752
BtsCI GGATG 6 cut(s) 31, 371, 1351, 1426, 1463, 2314
BtsI GCAGTG 1 cut(s) 2319
BtsIMutI CAGTG 5 cut(s) 378, 730, 1324, 1596, 2319
Cac8I GCNNGC 3 cut(s) 755, 973, 977
CaiI CAGNNNCTG 2 cut(s) 2348, 2408
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 1000, 1579
Cfr13I GGNCC 3 cut(s) 71, 728, 1999
CseI GACGC 1 cut(s) 724
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviAII CATG 9 cut(s) 98, 205, 500, 680, 761, 911, 934, 1001, 1292
DdeI CTNAG 7 cut(s) 544, 843, 1122, 1182, 1362, 1855, 2097
Eam1104I CTCTTC 5 cut(s) 942, 960, 1066, 1686, 2292
EarI CTCTTC 5 cut(s) 942, 960, 1066, 1686, 2292
EciI GGCGGA 2 cut(s) 69, 1370
Eco130I CCWWGG 1 cut(s) 1194
Eco31I GGTCTC 1 cut(s) 2093
Eco47I GGWCC 2 cut(s) 71, 1999
Eco57I CTGAAG 3 cut(s) 296, 554, 2547
Eco72I CACGTG 2 cut(s) 869, 992
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 1189
EcoNI CCTNNNNNAGG 1 cut(s) 2352
EcoO109I RGGNCCY 1 cut(s) 1999
EcoO65I GGTNACC 1 cut(s) 1189
EcoRI GAATTC 3 cut(s) 102, 595, 1135
EcoRII CCWGG 3 cut(s) 1415, 1714, 2347
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 1194
Esp3I CGTCTC 1 cut(s) 1932
FaeI CATG 9 cut(s) 101, 208, 503, 683, 764, 914, 937, 1004, 1295
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 1100
FatI CATG 9 cut(s) 97, 204, 499, 679, 760, 910, 933, 1000, 1291
FauNDI CATATG 2 cut(s) 586, 2304
FblI GTMKAC 2 cut(s) 873, 1729
Fnu4HI GCNGC 4 cut(s) 249, 701, 1107, 1623
FokI GGATG 6 cut(s) 38, 378, 1358, 1433, 1470, 2321
Fsp4HI GCNGC 4 cut(s) 249, 701, 1107, 1623
FspBI CTAG 7 cut(s) 1173, 1332, 1391, 1794, 2207, 2238, 2292
GlaI GCGC 2 cut(s) 999, 1578
GluI GCNGC 4 cut(s) 249, 701, 1107, 1623
GsaI CCCAGC 2 cut(s) 1287, 1546
HaeIII GGCC 2 cut(s) 730, 1639
HapII CCGG 1 cut(s) 2103
HgaI GACGC 1 cut(s) 724
HhaI GCGC 2 cut(s) 1000, 1579
Hin1II CATG 9 cut(s) 101, 208, 503, 683, 764, 914, 937, 1004, 1295
Hin6I GCGC 2 cut(s) 998, 1577
HinP1I GCGC 2 cut(s) 998, 1577
HindIII AAGCTT 1 cut(s) 407
HpaII CCGG 1 cut(s) 2103
HphI GGTGA 3 cut(s) 1888, 2390, 2432
Hpy166II GTNNAC 7 cut(s) 9, 874, 919, 1368, 1539, 1730, 2400
Hpy188III TCNNGA 4 cut(s) 524, 651, 680, 1609
Hpy8I GTNNAC 7 cut(s) 9, 874, 919, 1368, 1539, 1730, 2400
Hpy99I CGWCG 2 cut(s) 692, 740
HpyAV CCTTC 9 cut(s) 282, 342, 578, 584, 1128, 1990, 2012, 2187, 2368
HpyCH4III ACNGT 9 cut(s) 15, 373, 725, 1339, 1453, 1477, 1600, 1650, 2010
HpyCH4IV ACGT 7 cut(s) 690, 868, 876, 991, 1571, 1879, 2203
HpyF10VI GCNNNNNNNGC 2 cut(s) 1583, 2414
HpyF3I CTNAG 7 cut(s) 544, 843, 1122, 1182, 1362, 1855, 2097
HpySE526I ACGT 7 cut(s) 690, 868, 876, 991, 1571, 1879, 2203
Hsp92II CATG 9 cut(s) 101, 208, 503, 683, 764, 914, 937, 1004, 1295
HspAI GCGC 2 cut(s) 998, 1577
KpnI GGTACC 1 cut(s) 2200
LguI GCTCTTC 2 cut(s) 1066, 2292
LmnI GCTCC 4 cut(s) 349, 659, 833, 1497
Lsp1109I GCAGC 3 cut(s) 235, 712, 1093
LweI GCATC 5 cut(s) 778, 1003, 1336, 2299, 2395
MaeI CTAG 7 cut(s) 1173, 1332, 1391, 1794, 2207, 2238, 2292
MaeII ACGT 7 cut(s) 690, 868, 876, 991, 1571, 1879, 2203
MaeIII GTNAC 9 cut(s) 93, 238, 864, 987, 1168, 1189, 1287, 1930, 2479
MbiI CCGCTC 2 cut(s) 836, 1901
MfeI CAATTG 1 cut(s) 2136
MflI RGATCY 2 cut(s) 777, 1357
MhlI GDGCHC 1 cut(s) 921
MlyI GAGTC 5 cut(s) 569, 656, 821, 1363, 1728
MmeI TCCRAC 5 cut(s) 937, 1435, 1723, 2204, 2446
Mph1103I ATGCAT 1 cut(s) 501
MroXI GAANNNNTTC 1 cut(s) 1667
MseI TTAA 6 cut(s) 66, 261, 1305, 1479, 1485, 2337
MslI CAYNNNNRTG 1 cut(s) 2307
MspA1I CMGCKG 2 cut(s) 775, 1106
MspCI CTTAAG 1 cut(s) 2336
MspI CCGG 1 cut(s) 2103
MspR9I CCNGG 3 cut(s) 1417, 1716, 2349
MunI CAATTG 1 cut(s) 2136
Mva1269I GAATGC 2 cut(s) 1669, 2112
MvaI CCWGG 3 cut(s) 1417, 1716, 2349
MvnI CGCG 2 cut(s) 735, 998
MwoI GCNNNNNNNGC 2 cut(s) 1583, 2414
NdeI CATATG 2 cut(s) 586, 2304
NlaIII CATG 9 cut(s) 101, 208, 503, 683, 764, 914, 937, 1004, 1295
NlaIV GGNNCC 3 cut(s) 351, 1359, 2198
NmeAIII GCCGAG 1 cut(s) 831
NmuCI GTSAC 4 cut(s) 238, 864, 987, 1287
NsiI ATGCAT 1 cut(s) 501
NspI RCATGY 4 cut(s) 503, 764, 914, 1295
NspV TTCGAA 1 cut(s) 1603
PagI TCATGA 1 cut(s) 679
PciI ACATGT 2 cut(s) 760, 910
PciSI GCTCTTC 2 cut(s) 1066, 2292
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PctI GAATGC 2 cut(s) 1669, 2112
PdmI GAANNNNTTC 1 cut(s) 1667
PfeI GAWTC 9 cut(s) 316, 527, 1046, 1100, 1605, 1812, 2146, 2170, 2381
PflMI CCANNNNNTGG 1 cut(s) 1279
PkrI GCNGC 4 cut(s) 250, 702, 1108, 1624
PleI GAGTC 5 cut(s) 568, 655, 820, 1363, 1727
PmaCI CACGTG 2 cut(s) 869, 992
PmlI CACGTG 2 cut(s) 869, 992
PpsI GAGTC 5 cut(s) 568, 655, 820, 1363, 1727
Ppu21I YACGTR 2 cut(s) 869, 992
PpuMI RGGWCCY 1 cut(s) 1999
PscI ACATGT 2 cut(s) 760, 910
PshBI ATTAAT 3 cut(s) 66, 261, 1305
Psp1406I AACGTT 1 cut(s) 1571
Psp5II RGGWCCY 1 cut(s) 1999
Psp6I CCWGG 3 cut(s) 1415, 1714, 2347
PspCI CACGTG 2 cut(s) 869, 992
PspEI GGTNACC 1 cut(s) 1189
PspFI CCCAGC 2 cut(s) 1283, 1542
PspGI CCWGG 3 cut(s) 1415, 1714, 2347
PspN4I GGNNCC 3 cut(s) 351, 1359, 2198
PspPI GGNCC 3 cut(s) 71, 728, 1999
PspPPI RGGWCCY 1 cut(s) 1999
PstNI CAGNNNCTG 2 cut(s) 2348, 2408
PsuI RGATCY 2 cut(s) 777, 1357
RseI CAYNNNNRTG 1 cut(s) 2307
SapI GCTCTTC 2 cut(s) 1066, 2292
SaqAI TTAA 6 cut(s) 66, 261, 1305, 1479, 1485, 2337
SatI GCNGC 4 cut(s) 249, 701, 1107, 1623
Sau96I GGNCC 3 cut(s) 71, 728, 1999
ScaI AGTACT 1 cut(s) 1336
SchI GAGTC 5 cut(s) 569, 656, 821, 1363, 1728
ScrFI CCNGG 3 cut(s) 1417, 1716, 2349
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 5 cut(s) 778, 1003, 1336, 2299, 2395
SfcI CTRYAG 4 cut(s) 216, 1251, 1581, 1964
SfuI TTCGAA 1 cut(s) 1603
SinI GGWCC 2 cut(s) 71, 1999
SmiMI CAYNNNNRTG 1 cut(s) 2307
SmlI CTYRAG 2 cut(s) 1371, 2336
SmoI CTYRAG 2 cut(s) 1371, 2336
SsiI CCGC 7 cut(s) 54, 775, 836, 1104, 1355, 1622, 1899
SspI AATATT 2 cut(s) 224, 1165
SspMI CTAG 7 cut(s) 1173, 1332, 1391, 1794, 2207, 2238, 2292
StyD4I CCNGG 3 cut(s) 1415, 1714, 2347
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 9 cut(s) 15, 373, 725, 1339, 1453, 1477, 1600, 1650, 2010
TaiI ACGT 7 cut(s) 693, 871, 879, 994, 1574, 1882, 2206
TatI WGTACW 5 cut(s) 368, 694, 1334, 1339, 2080
TauI GCSGC 1 cut(s) 1625
TfiI GAWTC 9 cut(s) 316, 527, 1046, 1100, 1605, 1812, 2146, 2170, 2381
Tru1I TTAA 6 cut(s) 66, 261, 1305, 1479, 1485, 2337
Tru9I TTAA 6 cut(s) 66, 261, 1305, 1479, 1485, 2337
TscAI CASTG 5 cut(s) 378, 730, 1324, 1603, 2319
TseFI GTSAC 4 cut(s) 238, 864, 987, 1287
TseI GCWGC 3 cut(s) 248, 700, 1106
Tsp45I GTSAC 4 cut(s) 238, 864, 987, 1287
TspGWI ACGGA 3 cut(s) 176, 415, 846
TspRI CASTG 5 cut(s) 378, 730, 1324, 1603, 2319
Van91I CCANNNNNTGG 1 cut(s) 1279
Vha464I CTTAAG 1 cut(s) 2336
VneI GTGCAC 1 cut(s) 917
VpaK11BI GGWCC 2 cut(s) 71, 1999
VspI ATTAAT 3 cut(s) 66, 261, 1305
XagI CCTNNNNNAGG 1 cut(s) 2352
XapI RAATTY 6 cut(s) 102, 595, 604, 614, 623, 1135
XceI RCATGY 4 cut(s) 503, 764, 914, 1295
XcmI CCANNNNNNNNNTGG 2 cut(s) 47, 1280
XmiI GTMKAC 2 cut(s) 873, 1729
XmnI GAANNNNTTC 1 cut(s) 1667
XspI CTAG 7 cut(s) 1173, 1332, 1391, 1794, 2207, 2238, 2292
ZrmI AGTACT 1 cut(s) 1336
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.