FvH4_3g24071

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000071
Physical Location & Seq
Forward (+)
17111303 .. 17114053
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24071.t1

Sequence Viewer

Length: 2751 bp
ATGCCTAAGTACCTTTGGGGAGAAGCCGTGTTGTGTGCGTCTCACCTAATCAATCGTCTCCCGTCAGCTCCTCTTCAAGGTCGTGTTCCCTTTGATGTCTTGTCTCAATACGTTCCTATCCCGTCATCAAACACTCTTCCTGCTCATGTTTTTGGTTGTGTTGCTTATGTCCATTTGTATAAGAATCAAAGGTCAAAATTGGATGCTAGAGCCCTTAAGTGTGTGTTTGTAGGGTATGGTTCCCATCAGAAAGGTTATAAGTGTTATCATCCTCCATCCCAAAGAATATATGTCACCATGGATGTTACATTTAGTGAAGATACATGTTATTTTTTGCCTCCTGTAACTCCTCTCCAGGGGGAGAAGTCATATTTTTATGAAGATTTGTTTAGCGAGTCAACCAGGAGTCAAGGTTTTGAGGAGTCACCAAAGGACAATAAAGGGAACGAGTCAGTTTTGCAGAAACATAAAAGAGGCAATGAACATGAGCGACTGTCGTCATCTGTTGAGTTCAGACCCGATACCGGTGTTCCGCCGGAGCTCCCAGGCTCGCCGGCGCTGTCTCCGTCCGAGTGTCTAGCCTCACCGGCCCGTTCTCCGTCCGAGGGCCCAGCCAATAGTGGTTCTCCAACCGGATTTCAACATATTTCCGATCAACTGACCGGAGCAGAAAGCAGAGAGTTAGGGTTCCCAGATCAGACTAGCTCCACCTGTCCATTAATCGGAGACAATCGATCTCATCTTCCTGTCTCGTCGGTTTCCGGCGCCCTGACTCCGCCGGCCGTAGGCGACCTTCTAGAGAGCAGCGTGACCGGTTGTCATCCAGTTTTGGGCAGTGACCCTGTCAGTTCTGCATCAACAAATCAATCAGGGTCAGATTCTAGTCAAGGCCATGATTCGTCAGTGAGGAAGCAGATTTCTGATCAAGGCCATGCTTCGTCAGTCAGGGAGCAAAATTCGAATGTGAACAGTGCTCCTGCCCCTCCCTTACCTTTACAGGTCTCTCCGGCTCATGCATCACCCGAGGTACGTTCTACTCATTCTTTCGTGTCTGTTAAACAATTACCTAACCGTGTCACTCGTGGTCAACCTCCAAAACACTATGAACCTACCCTTAGTGCAAAAGCTCGGTATCTTGTTGCTAATTATGTGTCAACCCATAGATTGTCTAAACCATATGCTGCGTTTGTGAATCAATTATCTACTGTATCTCTCCCTAGTAAAGTGCAGGATGCTATGCGTGATGTTAAGTGGATGCAAGCAATGAAAGTTGAGATGGAAGCTCTTGAGAAGAATTGTACGTGGGAGTTGGTACCATTACCACCGGGTAAGAAGACAGTTGGTTGTCGGTGGGTGTATAGTGTGAAACATAATCCTAATGGTTCAGTGGATAGGTACAAGGCAAGGTTAGTCGCTAAAGGCTACACTCAGAAGTATGGGGTGGATTATGACGAGACGTTTGCACCAGTGGCAAAAATTAATACAATACGAGTACTTCTTTCGTTAGCCGTTAATCTTGATTGGCCTTTGCAGCAATTTGATGTTAAGAATGCTTTCTTGCATAGTGATTTGCATGAAGAAGTCTATATGGATTTACCTCTTGGATATGGTACTTCTACTGGAGAGCAGGTAGTGTGCAAGTTGAAGAAGGCCCTTTACGGTCTAAAGCAATCACCCAGAGCATGGTTTGGACGTTTTTCTAAGTTCATGAAGAGGATTGGATACCGACAGAGTAACTCAGATCACACTTTGTTTCTTAAACACCGGTGTGGTAAGGTGACAGCTTTAATTATTTATGTAGATGATATGGTTGTGACAGGTGATGACAATGAGGAAATTCAGAGATTACAAGATTTGTTGTCCTCTGAATTTGAGATGAAAGATTTGGGCAGTTTGAAGTATTTCTTGGGAATTGAAGTTGCTCGTGGGAAGGATTGTATTATGCTAAGTCAGAGAAAATATGTCCTAGATTTGTTAGCAGAAACGGGCATGCTTGACTGTAAACCGGCTGATACCCCTATTGAACAAAATCATCAGTTAGCAGAGTATTTGGATCAAGTGCCTACAAATAAGGCACGATATCAGAGGTTAGTTGGCCGATTGATTTATTTGTCTCACACTAGACCAGATTTAGCCTATGCAGTTAGTGTTGTGAGTCAGTTTATGCATAATCCTAGTGAAGCTCATATGGAAGCTGTATTTCGTATCTTGAAGTATTTGAAGTCTGCTCCAGGGAAGGGGTTAATATTTTCAAAACACAAGCACTTGGATGTTTCTGGGTACACAGACTCAGACTGGGCAGGTAATATTACAGATCGCAGGTCCACTTCCGGCTACTTCACTTTCGTTGGTGGTAATTTGGTTACTTGGAAAAGTAAGAAACAAAAGGTGGTAGCTCGCTCCAGTGCTGAAGCAGAGTATCGTGGAATGGCTCTTGGAGTATGTGAGATGTTGTGGTTGAGACATTTGTTGAGAGATTTGGGATTCAGACAAAAGAAGGCGATGCCTCTATATTGTGATAATAAAGCTACTGTTGAAATTGCTCATAATCCAGTTCAGCATGACAAAACAAAACATGTGGAGGTCGATCGACATTTCATTAAGGAGAAGTTGGATCGCCAGATTATTGTGTTCCCATTTGTACCTACCGAGGAGCAGCTGGCAGACATTCTTACGAAAGCAGTCTTTAGTAAGATGTTTTATGACTCACTTGACAAGTTGGGCATCTGTGATCTGTATGCTCCAACTTGA

Protein Analysis

917

Amino Acids

102.22

Weight (kDa)

8.68

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 53 - 110 3.3e-23 Retroviral polymerase SH3-like domain
RVT_2 PF07727 433 - 675 9.7e-71 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
Acc36I ACCTGC 3 cut(s) 1618, 2291, 2310
Acc65I GGTACC 1 cut(s) 1312
AccB1I GGYRCC 2 cut(s) 764, 1312
AccB7I CCANNNNNTGG 1 cut(s) 1683
AciI CCGC 2 cut(s) 533, 776
AclWI GGATC 2 cut(s) 2061, 2622
AcoI YGGCCR 2 cut(s) 780, 2095
AcsI RAATTY 3 cut(s) 955, 1836, 1868
AcuI CTGAAG 1 cut(s) 2430
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 9 cut(s) 11, 1029, 1300, 1314, 1397, 1494, 1612, 2282, 2643
AfiI CCNNNNNNNGG 9 cut(s) 77, 356, 524, 605, 722, 785, 830, 1683, 2237
AflII CTTAAG 1 cut(s) 215
AflIII ACRYGT 2 cut(s) 323, 2575
AgeI ACCGGT 3 cut(s) 524, 812, 1764
AhdI GACNNNNNGTC 1 cut(s) 816
AjnI CCWGG 4 cut(s) 354, 401, 544, 2230
AjuI GAANNNNNNNTTGG 2 cut(s) 1889, 1921
AleI CACNNNNGTG 1 cut(s) 1767
Alw21I GWGCWC 2 cut(s) 543, 976
AlwI GGATC 2 cut(s) 2061, 2622
Ama87I CYCGRG 1 cut(s) 1022
AoxI GGCC 8 cut(s) 588, 607, 780, 889, 928, 1523, 1650, 2095
ApaI GGGCCC 1 cut(s) 611
ApeKI GCWGC 4 cut(s) 804, 1181, 1531, 2656
ApoI RAATTY 3 cut(s) 955, 1836, 1868
ArsI GACNNNNNNTTYG 2 cut(s) 1442, 1474
AseI ATTAAT 2 cut(s) 719, 1479
AsiGI ACCGGT 3 cut(s) 524, 812, 1764
Asp700I GAANNNNTTC 2 cut(s) 1553, 1901
Asp718I GGTACC 1 cut(s) 1312
AspLEI GCGC 2 cut(s) 559, 767
AspS9I GGNCC 5 cut(s) 589, 607, 608, 1651, 2322
AsuC2I CCSGG 1 cut(s) 1326
AsuHPI GGTGA 8 cut(s) 35, 286, 417, 576, 1011, 1665, 1789, 1832
AsuII TTCGAA 1 cut(s) 959
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 2322
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 2 cut(s) 764, 1312
BanII GRGCYC 3 cut(s) 214, 543, 611
BarI GAAGNNNNNNTAC 4 cut(s) 777, 809, 1640, 1672
BauI CACGAG 2 cut(s) 1080, 1923
BbsI GAAGAC 1 cut(s) 1340
Bbv12I GWGCWC 2 cut(s) 543, 976
BbvI GCAGC 4 cut(s) 816, 1168, 1543, 2668
BccI CCATC 3 cut(s) 252, 283, 1270
BceAI ACGGC 3 cut(s) 11, 767, 1493
BcgI CGANNNNNNTGC 2 cut(s) 1442, 1476
BciT130I CCWGG 4 cut(s) 356, 403, 546, 2232
BciVI GTATCC 1 cut(s) 1715
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 1 cut(s) 1326
BfaI CTAG 9 cut(s) 207, 578, 702, 797, 882, 1218, 1967, 2121, 2175
BfoI RGCGCY 2 cut(s) 560, 768
BfrI CTTAAG 1 cut(s) 215
BfuAI ACCTGC 3 cut(s) 1618, 2291, 2310
BfuI GTATCC 1 cut(s) 1715
BglI GCCNNNNNGGC 1 cut(s) 587
BisI GCNGC 4 cut(s) 805, 1182, 1532, 2657
BlsI GCNGC 4 cut(s) 806, 1183, 1533, 2658
BmcAI AGTACT 1 cut(s) 1494
Bme1390I CCNGG 5 cut(s) 356, 403, 546, 1326, 2232
Bme18I GGWCC 1 cut(s) 2322
BmeRI GACNNNNNGTC 1 cut(s) 816
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 5 cut(s) 589, 607, 608, 1651, 2322
BmiI GGNNCC 5 cut(s) 241, 609, 689, 766, 1314
BmrFI CCNGG 5 cut(s) 356, 403, 546, 1326, 2232
BmrI ACTGGG 1 cut(s) 2305
BmsI GCATC 7 cut(s) 193, 863, 1025, 1222, 1245, 2493, 2733
BmuI ACTGGG 1 cut(s) 2305
BoxI GACNNNNGTC 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 1340
BpmI CTGGAG 4 cut(s) 338, 1641, 2214, 2386
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 1 cut(s) 1307
BpuMI CCSGG 1 cut(s) 1326
Bsa29I ATCGAT 1 cut(s) 733
BsaAI YACGTR 1 cut(s) 1302
BsaHI GRCGYC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 1006
BsaJI CCNNGG 7 cut(s) 297, 355, 544, 603, 1023, 2231, 2649
BsaWI WCCGGW 5 cut(s) 524, 632, 662, 812, 1764
BsaXI ACNNNNNCTCC 6 cut(s) 12, 42, 397, 427, 1614, 1644
Bsc4I CCNNNNNNNGG 9 cut(s) 77, 356, 524, 605, 722, 785, 830, 1683, 2237
Bse118I RCCGGY 7 cut(s) 524, 553, 586, 778, 812, 1764, 2005
Bse1I ACTGG 6 cut(s) 824, 1466, 1624, 2300, 2403, 2552
Bse3DI GCAATG 2 cut(s) 484, 1269
BseBI CCWGG 4 cut(s) 356, 403, 546, 2232
BseCI ATCGAT 1 cut(s) 733
BseDI CCNNGG 7 cut(s) 297, 355, 544, 603, 1023, 2231, 2649
BseGI GGATG 8 cut(s) 208, 268, 275, 307, 820, 1237, 1260, 2275
BseLI CCNNNNNNNGG 9 cut(s) 77, 356, 524, 605, 722, 785, 830, 1683, 2237
BseMI GCAATG 2 cut(s) 484, 1269
BseMII CTCAG 3 cut(s) 1442, 1752, 2304
BseNI ACTGG 6 cut(s) 824, 1466, 1624, 2300, 2403, 2552
BseRI GAGGAG 4 cut(s) 60, 339, 434, 2666
BseSI GKGCMC 1 cut(s) 611
BseX3I CGGCCG 1 cut(s) 780
BseXI GCAGC 4 cut(s) 816, 1168, 1543, 2668
BseYI CCCAGC 1 cut(s) 610
BsgI GTGCAG 1 cut(s) 1247
Bsh1285I CGRYCG 2 cut(s) 783, 2590
BshFI GGCC 8 cut(s) 590, 609, 782, 891, 930, 1525, 1652, 2097
BshNI GGYRCC 2 cut(s) 764, 1312
BshTI ACCGGT 3 cut(s) 524, 812, 1764
BshVI ATCGAT 1 cut(s) 733
BsiEI CGRYCG 2 cut(s) 783, 2590
BsiHKAI GWGCWC 2 cut(s) 543, 976
BsiHKCI CYCGRG 1 cut(s) 1022
BslI CCNNNNNNNGG 9 cut(s) 77, 356, 524, 605, 722, 785, 830, 1683, 2237
BsmBI CGTCTC 3 cut(s) 45, 62, 1448
BsmI GAATGC 1 cut(s) 1555
BsnI GGCC 8 cut(s) 590, 609, 782, 891, 930, 1525, 1652, 2097
Bso31I GGTCTC 1 cut(s) 1006
BsoBI CYCGRG 1 cut(s) 1022
Bsp119I TTCGAA 1 cut(s) 959
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 4 cut(s) 214, 543, 611, 976
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 2 cut(s) 533, 776
BspANI GGCC 8 cut(s) 590, 609, 782, 891, 930, 1525, 1652, 2097
BspCNI CTCAG 3 cut(s) 1441, 1751, 2303
BspDI ATCGAT 1 cut(s) 733
BspHI TCATGA 1 cut(s) 1707
BspLI GGNNCC 5 cut(s) 241, 609, 689, 766, 1314
BspMI ACCTGC 3 cut(s) 1618, 2291, 2310
BspPI GGATC 2 cut(s) 2061, 2622
BspT104I TTCGAA 1 cut(s) 959
BspT107I GGYRCC 2 cut(s) 764, 1312
BspTI CTTAAG 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 1006
BsrDI GCAATG 2 cut(s) 484, 1269
BsrFI RCCGGY 7 cut(s) 524, 553, 586, 778, 812, 1764, 2005
BsrI ACTGG 6 cut(s) 824, 1466, 1624, 2300, 2403, 2552
BssAI RCCGGY 7 cut(s) 524, 553, 586, 778, 812, 1764, 2005
BssECI CCNNGG 7 cut(s) 297, 355, 544, 603, 1023, 2231, 2649
BssNI GRCGYC 1 cut(s) 765
BssSI CACGAG 2 cut(s) 1080, 1923
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 2 cut(s) 1080, 1923
Bst2UI CCWGG 4 cut(s) 356, 403, 546, 2232
Bst4CI ACNGT 8 cut(s) 495, 971, 1073, 1207, 1339, 1661, 2000, 2533
Bst6I CTCTTC 3 cut(s) 78, 141, 1706
BstACI GRCGYC 1 cut(s) 765
BstAFI CTTAAG 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 1302
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 7 cut(s) 551, 555, 780, 1260, 1991, 2398, 2661
BstDEI CTNAG 7 cut(s) 6, 1115, 1428, 1701, 1738, 1946, 2290
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 1 cut(s) 75
BstF5I GGATG 8 cut(s) 208, 268, 275, 307, 820, 1237, 1260, 2275
BstH2I RGCGCY 2 cut(s) 560, 768
BstHHI GCGC 2 cut(s) 559, 767
BstMCI CGRYCG 2 cut(s) 783, 2590
BstMWI GCNNNNNNNGC 3 cut(s) 587, 1469, 1531
BstNI CCWGG 4 cut(s) 356, 403, 546, 2232
BstNSI RCATGY 3 cut(s) 327, 1993, 2579
BstPAI GACNNNNGTC 1 cut(s) 496
BstSCI CCNGG 5 cut(s) 354, 401, 544, 1324, 2230
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 4 cut(s) 816, 1168, 1543, 2668
BstV2I GAAGAC 1 cut(s) 1340
BstZI CGGCCG 1 cut(s) 780
Bsu15I ATCGAT 1 cut(s) 733
BsuI GTATCC 1 cut(s) 1715
BsuRI GGCC 8 cut(s) 590, 609, 782, 891, 930, 1525, 1652, 2097
BsuTUI ATCGAT 1 cut(s) 733
BtgI CCRYGG 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 2516
BtsCI GGATG 8 cut(s) 208, 268, 275, 307, 820, 1237, 1260, 2275
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 6 cut(s) 841, 909, 976, 1392, 1473, 2410
BveI ACCTGC 3 cut(s) 1618, 2291, 2310
Cac8I GCNNGC 7 cut(s) 551, 555, 780, 1260, 1991, 2398, 2661
CciI TCATGA 1 cut(s) 1707
CfoI GCGC 2 cut(s) 559, 767
Cfr10I RCCGGY 7 cut(s) 524, 553, 586, 778, 812, 1764, 2005
Cfr13I GGNCC 5 cut(s) 589, 607, 608, 1651, 2322
ClaI ATCGAT 1 cut(s) 733
CseI GACGC 1 cut(s) 27
Csp6I GTAC 9 cut(s) 10, 1028, 1299, 1313, 1396, 1493, 1611, 2281, 2642
CspAI ACCGGT 3 cut(s) 524, 812, 1764
CspCI CAANNNNNGTGG 2 cut(s) 2559, 2594
CviQI GTAC 9 cut(s) 10, 1028, 1299, 1313, 1396, 1493, 1611, 2281, 2642
DdeI CTNAG 7 cut(s) 6, 1115, 1428, 1701, 1738, 1946, 2290
DinI GGCGCC 1 cut(s) 766
DriI GACNNNNNGTC 1 cut(s) 816
EaeI YGGCCR 2 cut(s) 780, 2095
EagI CGGCCG 1 cut(s) 780
Eam1104I CTCTTC 3 cut(s) 78, 141, 1706
Eam1105I GACNNNNNGTC 1 cut(s) 816
EarI CTCTTC 3 cut(s) 78, 141, 1706
EciI GGCGGA 2 cut(s) 522, 765
Ecl136II GAGCTC 1 cut(s) 541
EclXI CGGCCG 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 3 cut(s) 214, 543, 611
Eco31I GGTCTC 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 2081
Eco47I GGWCC 1 cut(s) 2322
Eco52I CGGCCG 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 2430
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 75
EcoO109I RGGNCCY 2 cut(s) 607, 1651
EcoRII CCWGG 4 cut(s) 354, 401, 544, 2230
EcoRV GATATC 1 cut(s) 2081
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 2 cut(s) 1018, 2169
EcoT38I GRGCYC 3 cut(s) 214, 543, 611
EgeI GGCGCC 1 cut(s) 766
EheI GGCGCC 1 cut(s) 766
ErhI CCWWGG 1 cut(s) 297
Esp3I CGTCTC 3 cut(s) 45, 62, 1448
FalI AAGNNNNNCTT 2 cut(s) 1889, 1921
FauNDI CATATG 2 cut(s) 1177, 2187
FbaI TGATCA 1 cut(s) 922
Fnu4HI GCNGC 4 cut(s) 805, 1182, 1532, 2657
FokI GGATG 8 cut(s) 215, 255, 262, 314, 807, 1244, 1267, 2282
FriOI GRGCYC 3 cut(s) 214, 543, 611
Fsp4HI GCNGC 4 cut(s) 805, 1182, 1532, 2657
FspBI CTAG 9 cut(s) 207, 578, 702, 797, 882, 1218, 1967, 2121, 2175
GlaI GCGC 2 cut(s) 558, 766
GluI GCNGC 4 cut(s) 805, 1182, 1532, 2657
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 4 cut(s) 338, 1641, 2214, 2386
HaeII RGCGCY 2 cut(s) 560, 768
HaeIII GGCC 8 cut(s) 590, 609, 782, 891, 930, 1525, 1652, 2097
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 559, 767
Hin1I GRCGYC 1 cut(s) 765
Hin6I GCGC 2 cut(s) 557, 765
HinP1I GCGC 2 cut(s) 557, 765
HincII GTYRAC 3 cut(s) 399, 1088, 1155
HindII GTYRAC 3 cut(s) 399, 1088, 1155
HphI GGTGA 8 cut(s) 35, 286, 417, 576, 1011, 1665, 1789, 1832
Hpy166II GTNNAC 7 cut(s) 399, 967, 1088, 1155, 2003, 2283, 2325
Hpy188III TCNNGA 5 cut(s) 797, 1286, 1517, 1708, 2209
Hpy8I GTNNAC 7 cut(s) 399, 967, 1088, 1155, 2003, 2283, 2325
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 5 cut(s) 803, 1642, 1924, 2230, 2491
HpyCH4III ACNGT 8 cut(s) 495, 971, 1073, 1207, 1339, 1661, 2000, 2533
HpyCH4IV ACGT 5 cut(s) 111, 1030, 1301, 1457, 1693
HpyF10VI GCNNNNNNNGC 3 cut(s) 587, 1469, 1531
HpyF3I CTNAG 7 cut(s) 6, 1115, 1428, 1701, 1738, 1946, 2290
HpySE526I ACGT 5 cut(s) 111, 1030, 1301, 1457, 1693
Hsp92I GRCGYC 1 cut(s) 765
HspAI GCGC 2 cut(s) 557, 765
KasI GGCGCC 1 cut(s) 764
KpnI GGTACC 1 cut(s) 1316
KroI GCCGGC 2 cut(s) 553, 778
KroNI GCCGGC 2 cut(s) 555, 780
Ksp22I TGATCA 1 cut(s) 922
Lsp1109I GCAGC 4 cut(s) 816, 1168, 1543, 2668
LweI GCATC 7 cut(s) 193, 863, 1025, 1222, 1245, 2493, 2733
MaeI CTAG 9 cut(s) 207, 578, 702, 797, 882, 1218, 1967, 2121, 2175
MaeII ACGT 5 cut(s) 111, 1030, 1301, 1457, 1693
MhlI GDGCHC 4 cut(s) 214, 543, 611, 976
Mly113I GGCGCC 1 cut(s) 765
MlyI GAGTC 8 cut(s) 404, 415, 431, 458, 766, 2164, 2282, 2699
MmeI TCCRAC 2 cut(s) 653, 2592
Mph1103I ATGCAT 2 cut(s) 1018, 2169
MreI CGCCGGCG 1 cut(s) 553
MroNI GCCGGC 2 cut(s) 553, 778
MroXI GAANNNNTTC 2 cut(s) 1553, 1901
MslI CAYNNNNRTG 2 cut(s) 1767, 2268
MspA1I CMGCKG 1 cut(s) 2659
MspCI CTTAAG 1 cut(s) 215
MspR9I CCNGG 5 cut(s) 356, 403, 546, 1326, 2232
Mva1269I GAATGC 1 cut(s) 1555
MvaI CCWGG 4 cut(s) 356, 403, 546, 2232
MwoI GCNNNNNNNGC 3 cut(s) 587, 1469, 1531
NaeI GCCGGC 2 cut(s) 555, 780
NarI GGCGCC 1 cut(s) 765
NciI CCSGG 1 cut(s) 1326
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 2 cut(s) 1177, 2187
NgoMIV GCCGGC 2 cut(s) 553, 778
NlaIV GGNNCC 5 cut(s) 241, 609, 689, 766, 1314
NmuCI GTSAC 7 cut(s) 292, 423, 808, 836, 1075, 1777, 1813
NsiI ATGCAT 2 cut(s) 1018, 2169
NspI RCATGY 3 cut(s) 327, 1993, 2579
NspV TTCGAA 1 cut(s) 959
OliI CACNNNNGTG 1 cut(s) 1767
PaeI GCATGC 1 cut(s) 1993
PagI TCATGA 1 cut(s) 1707
PciI ACATGT 2 cut(s) 323, 2575
PctI GAATGC 1 cut(s) 1555
PdiI GCCGGC 2 cut(s) 555, 780
PdmI GAANNNNTTC 2 cut(s) 1553, 1901
PfeI GAWTC 5 cut(s) 184, 878, 896, 1192, 2484
PflFI GACNNNGTC 1 cut(s) 842
PflMI CCANNNNNTGG 1 cut(s) 1683
PinAI ACCGGT 3 cut(s) 524, 812, 1764
PkrI GCNGC 4 cut(s) 806, 1183, 1533, 2658
Ple19I CGATCG 1 cut(s) 2590
PleI GAGTC 8 cut(s) 403, 414, 430, 457, 766, 2163, 2282, 2699
PluTI GGCGCC 1 cut(s) 768
PpsI GAGTC 8 cut(s) 403, 414, 430, 457, 766, 2163, 2282, 2699
Ppu21I YACGTR 1 cut(s) 1302
PscI ACATGT 2 cut(s) 323, 2575
PshAI GACNNNNGTC 1 cut(s) 496
PshBI ATTAAT 2 cut(s) 719, 1479
PsiI TTATAA 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 4 cut(s) 354, 401, 544, 2230
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 4 cut(s) 354, 401, 544, 2230
PspN4I GGNNCC 5 cut(s) 241, 609, 689, 766, 1314
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 5 cut(s) 589, 607, 608, 1651, 2322
PsyI GACNNNGTC 1 cut(s) 842
PvuI CGATCG 1 cut(s) 2590
PvuII CAGCTG 1 cut(s) 2659
RsaI GTAC 9 cut(s) 11, 1029, 1300, 1314, 1397, 1494, 1612, 2282, 2643
RsaNI GTAC 9 cut(s) 10, 1028, 1299, 1313, 1396, 1493, 1611, 2281, 2642
RseI CAYNNNNRTG 2 cut(s) 1767, 2268
SacI GAGCTC 1 cut(s) 543
SatI GCNGC 4 cut(s) 805, 1182, 1532, 2657
Sau96I GGNCC 5 cut(s) 589, 607, 608, 1651, 2322
ScaI AGTACT 1 cut(s) 1494
SchI GAGTC 8 cut(s) 404, 415, 431, 458, 766, 2164, 2282, 2699
ScrFI CCNGG 5 cut(s) 356, 403, 546, 1326, 2232
SduI GDGCHC 4 cut(s) 214, 543, 611, 976
SfaNI GCATC 7 cut(s) 193, 863, 1025, 1222, 1245, 2493, 2733
SfoI GGCGCC 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 959
SgrAI CRCCGGYG 2 cut(s) 553, 1764
SinI GGWCC 1 cut(s) 2322
SmiMI CAYNNNNRTG 2 cut(s) 1767, 2268
SmlI CTYRAG 2 cut(s) 215, 1286
SmoI CTYRAG 2 cut(s) 215, 1286
SphI GCATGC 1 cut(s) 1993
SsiI CCGC 2 cut(s) 533, 776
SspDI GGCGCC 1 cut(s) 764
SspI AATATT 2 cut(s) 2247, 2308
SspMI CTAG 9 cut(s) 207, 578, 702, 797, 882, 1218, 1967, 2121, 2175
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 5 cut(s) 354, 401, 544, 1324, 2230
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 8 cut(s) 495, 971, 1073, 1207, 1339, 1661, 2000, 2533
TaiI ACGT 5 cut(s) 114, 1033, 1304, 1460, 1696
TaqI TCGA 4 cut(s) 733, 959, 2586, 2590
TatI WGTACW 1 cut(s) 1492
TfiI GAWTC 5 cut(s) 184, 878, 896, 1192, 2484
TscAI CASTG 6 cut(s) 841, 909, 976, 1392, 1473, 2410
TseFI GTSAC 7 cut(s) 292, 423, 808, 836, 1075, 1777, 1813
TseI GCWGC 4 cut(s) 804, 1181, 1531, 2656
Tsp45I GTSAC 7 cut(s) 292, 423, 808, 836, 1075, 1777, 1813
TspDTI ATGAA 9 cut(s) 393, 495, 1119, 1280, 1590, 1696, 1724, 1892, 2587
TspGWI ACGGA 2 cut(s) 555, 588
TspRI CASTG 6 cut(s) 841, 909, 976, 1392, 1473, 2410
Tth111I GACNNNGTC 1 cut(s) 842
Van91I CCANNNNNTGG 1 cut(s) 1683
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 2322
VspI ATTAAT 2 cut(s) 719, 1479
XagI CCTNNNNNAGG 1 cut(s) 75
XapI RAATTY 3 cut(s) 955, 1836, 1868
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 3 cut(s) 327, 1993, 2579
XmnI GAANNNNTTC 2 cut(s) 1553, 1901
XspI CTAG 9 cut(s) 207, 578, 702, 797, 882, 1218, 1967, 2121, 2175
ZrmI AGTACT 1 cut(s) 1494
Zsp2I ATGCAT 2 cut(s) 1018, 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.