Rw4G000280
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000071
Physical Location & Seq
Reverse (-)
660622 .. 671192
10571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000280.1

Sequence Viewer

Length: 3063 bp
ATGGCAGACGAACAAAAACTCTCTAACCCCTACTTCCTCCACCATTCAGATCATCCAGGTTTGGTTTTAATTTCCAAACCACTGAATGGAGATAATTATCCAACTTGGTTGCGAGCAATGACTATCGCTCTAAATGCCAAGAATAAGTTAGGCTTTGTTAATGGCACTATCAAGCCTCCTTCGGCAGAAACTCATCCAGATAATTATGATACATGGTCTCGATGCAATGACATGGTCCACTCATGGATTATTAATTCCATTGCTCCAGAGATCTCAGATAAACAACTTTCAGTTTCTGCTTACTACACCAAGTTGAAGACTTTATGGGATGAATTATCCTCCTACACAGATGCGTCTTCTTGTACCTGTGGAGCACAGCATGACCGACAAAAGCTTATGCAATTTCTAATGGGTTTGAATGAGTCCTACAGTGCTGCACGAGGACAAATCCTTCTCATGAATCATCTTCCTTCAGTTAGACAAGCTTATGGATCTATTTGCCAAGTTGAAAAACAAAGGCATCTCAGTGCGACATATACCACAGACTCAAGCACAAGTGCTGCCATGGCTGTCAGGAACAATCGATCAAATCAGTCTGCTCGACCAACACATCGCTCCTCTACCAATGATGGACGACATCTTGATCCAAACCAGCGTCGTTTGGGCTATTCTAAAGGCCGACCTAAATGCTCTTACAGTGGGGACATGGGACACTTTGTTGAAACTTGCTACAAATTAATTGGATATCCACCTGGTTACCCAAAAGAGAGAACAAATCGTTCTATGACTGATGGGAACCGAGAAAAAAATTCAGCAGCCAATCATCAATCCCAAACTAAGCCCAAGTCTGTGATATCAGGTGTTTCAGAGGCGCAACTTCAACAACTCTTGTCCATCCTCAGTGTCAAAGATAGCGGTGAAAATTCCCAAGCACATGCAGCAGTGGCTAAGCCAAAAACCCCACCAGGTTTGTCTAAAATTTCCTCTTGTCGCTGGATCATTGATAGCGGCGCGACGGACCATATGTCTTCATCCTCAAAATTATTTTTACATGGGGGAAAAAGTTGTTCATTGCCTCCAGTATTGTTACCTAGTGGGGAGAAAGCCCATATTGCTGCCAAAGGATCATTGCCTCTAAATTCTACATATTATTTGCATGATGTGCTATGTGTGCCTACATTCAAAGTCGATTTAATGTCCGATCTGGTTACGAAGAGGATGATTGGTTTGGGTAGGCAGCGTAATGGCCTATATTACTTGGTGGCACTAGCGACGAAGAAAACCTTGACCAACCCTCATTCTACCTCAAACAACCAGCCCGCTTATGTCGTTTTTCATGAAACATCTTTTCCTTATGCTTCTATCACGTCCAATACCAATTCTTCTTCTTTGGGTCCAAATTCTAGCCCCATTCCTTTCCCCCTCACTGACCCATCTCCCTTAGCTGACCCTACCCATCAGTCTGACCACATATCTCCCATTGACCCCGAAATTGACTCTCTTCTTCCTCCTTCTTCGATCGACACAGTCCCTCCACCGTCACCAACTGCGATCCCACCTGCGCTCCCATCCTCTGACGCCACCGAAAGCCCACCCATATCTCCCGCATCGCCGGTGCCACCCCCCGCATCACCTGGCCCCTTCCCAGACCCGCCTCTGCCTGAGCCTGAACCACCCTCACCCGCGCCTCTTCGTCGCTCCAGTCGTGCCACGGCGCCGCCGGTGAAATTCCGAGATTATGTCTGCTCCCAGGTCACGCTGCCTTCCTCCGACCCGTCATCTTCTTTGTTTCCCGGTCCAACTAAAGGAACTCGTTATCCCCTCTCCAACTATGTCTCCTACCACCGCTACAAGCTCGCCCACCGGTCTTTCATTGCCCAAATCAGTCAAGCAATCGAGCCCAGTTCCTATTCCGAAGCGGTTGTTCATCCTGAGTGGCAGGAGGCGATGAGAGCTGAATTGCACGCTCTCCAAGCAAATGGTACATGGACCCTCACTTCTCTCCCGGCCGGTAAGAAGCCAATTGATTGTCGTTGGGTTTATAAAATCAAACACCGGTCTGATGGTACCATTGAGCGGTACAAAGCCCGATTAGAAACCTTCTCTCCCACTGCCAAAATTATTTCTGTACGATGTTTACTTGCCTTGGCAGCAGCCCGGGGATGGTCACTTCACCAACTGGATGTCAATAACGCTTTCTTACATGGCGACCTCCATGAGGAAATCTATATGAAAGATTGGTCTGCCGCCTTCACAAGTCATTATATGGCTATTCGATCTGCTGGTTACATACAATCCAGGGCTGACTACTCTTTATTCACCAAGAGACAAGGCAAGTCTTTTACTGCCCTCTTGATATATGTTGATGATATTTTGATTACGGGAAATGATTCTGCAAGTATTGATGCACTCAAGCAATTTCTTCATCGTAATTTCCGTATTAAAGACCTTGGAGACTTAAAATATTTCCTTGGCATTGAGGTTTCTGCTTCGAAAAATGGGATTTTTATTTCTCAACGAAAATATGCGTTAGAGATTATTAAGGATGCAGGGTTGATGGGCGCTGCTCCTGTTGACACACCTATGGAACGAGGACTGAAGTTATCTGACATGAGGTGCCCCATTACTAGAAGATCAACCACAGGTTATTGTGTTTTTCTTGGTTCTTCGTTGGTTTCATGGAGATCAAAAAGACAGAAAACAGTCTCGCTTTCTTCAGCTGAAGCGGAGTATCGTGCAATGACAGGTGCCTGCTGTGAATTAACATGGCTTCGATATCTACTCAGAGACTTAGGAATATTACATCCAGAACCCGCCTTGCTATTTTGTGATAACAAGGCCGCGCTACACATTGCAGCGAATCCAGTTTTTCATGAGCGAACTCGTCATATTGAAATGGACTGCCACTACATCAGGGATAAAATCCAAGATGGGTCTGTTGTTACCAAGTTTGTCATTTCTGCTCATCAATTGGCGGATGTCCTCACTAAGGCCTTGGGCAAGGAAATATTCCTTCCTATGATTCGCAAGTTGGGAGTTCGAGATATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

1020

Amino Acids

112.54

Weight (kDa)

9.11

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 14 - 60 4.6e-21 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 332 - 400 1.6e-07 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 660 - 746 1.8e-18 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 760 - 863 2e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2043
AarI CACCTGC 1 cut(s) 1567
Acc36I ACCTGC 1 cut(s) 1567
Acc65I GGTACC 1 cut(s) 2066
AccB1I GGYRCC 5 cut(s) 1615, 1714, 2066, 2616, 2747
AccB7I CCANNNNNTGG 1 cut(s) 86
AccBSI CCGCTC 1 cut(s) 2077
AccII CGCG 3 cut(s) 1013, 1685, 2843
AclWI GGATC 5 cut(s) 499, 638, 1004, 1132, 1546
AcoI YGGCCR 1 cut(s) 2007
AcsI RAATTY 6 cut(s) 808, 922, 978, 1138, 1399, 1727
AcuI CTGAAG 4 cut(s) 456, 2618, 2700, 2742
AcyI GRCGYC 2 cut(s) 1578, 1715
AfaI GTAC 5 cut(s) 364, 1984, 2068, 2081, 2130
AfiI CCNNNNNNNGG 6 cut(s) 86, 1805, 2076, 2163, 2218, 2989
AgeI ACCGGT 2 cut(s) 1863, 2055
AgsI TTSAA 7 cut(s) 316, 418, 509, 722, 881, 1183, 2894
AhdI GACNNNNNGTC 1 cut(s) 1024
AjiI CACGTC 1 cut(s) 1368
AjnI CCWGG 6 cut(s) 55, 751, 964, 1633, 1749, 2297
AloI GAACNNNNNNTCC 2 cut(s) 1801, 1833
AluBI AGCT 6 cut(s) 394, 485, 1445, 1855, 1955, 2720
AluI AGCT 6 cut(s) 394, 485, 1445, 1855, 1955, 2720
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 6 cut(s) 222, 1840, 2320, 2448, 2710, 2781
AlwI GGATC 5 cut(s) 499, 638, 1004, 1132, 1546
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 2157
AoxI GGCC 6 cut(s) 676, 1246, 1636, 2007, 2838, 2991
ApoI RAATTY 6 cut(s) 808, 922, 978, 1138, 1399, 1727
AseI ATTAAT 2 cut(s) 252, 737
AsiGI ACCGGT 2 cut(s) 1863, 2055
Asp700I GAANNNNTTC 1 cut(s) 2389
Asp718I GGTACC 1 cut(s) 2066
AspLEI GCGC 7 cut(s) 874, 1013, 1564, 1687, 1717, 2564, 2845
AspS9I GGNCC 6 cut(s) 235, 1018, 1394, 1637, 1796, 1989
AsuC2I CCSGG 4 cut(s) 1794, 2006, 2158, 2159
AsuHPI GGTGA 7 cut(s) 929, 1533, 1623, 1671, 1735, 2165, 2311
AsuII TTCGAA 1 cut(s) 2492
AvaI CYCGRG 1 cut(s) 2157
AvaII GGWCC 5 cut(s) 235, 1018, 1394, 1796, 1989
BaeGI GKGCMC 1 cut(s) 2621
BanI GGYRCC 5 cut(s) 1615, 1714, 2066, 2616, 2747
BanII GRGCYC 1 cut(s) 1902
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 3 cut(s) 323, 348, 1020
Bbv12I GWGCWC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 1728
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 6 cut(s) 57, 753, 966, 1635, 1751, 2299
BcnI CCSGG 4 cut(s) 1794, 2006, 2158, 2159
BcoDI GTCTC 6 cut(s) 222, 1840, 2320, 2448, 2710, 2781
BfaI CTAG 4 cut(s) 1092, 1268, 1404, 2628
BfmI CTRYAG 1 cut(s) 427
BfoI RGCGCY 2 cut(s) 1718, 2565
BfuAI ACCTGC 1 cut(s) 1567
BglII AGATCT 1 cut(s) 270
BlpI GCTNAGC 1 cut(s) 948
Bme18I GGWCC 5 cut(s) 235, 1018, 1394, 1796, 1989
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmeT110I CYCGRG 1 cut(s) 2157
BmgBI CACGTC 1 cut(s) 1368
BmgT120I GGNCC 6 cut(s) 235, 1018, 1394, 1637, 1796, 1989
BmiI GGNNCC 9 cut(s) 797, 1395, 1617, 1639, 1716, 1991, 2068, 2618, 2749
BmrI ACTGGG 1 cut(s) 1896
BmsI GCATC 7 cut(s) 212, 340, 529, 1616, 1637, 2395, 2536
BmuI ACTGGG 1 cut(s) 1896
BpiI GAAGAC 3 cut(s) 323, 348, 1020
BpmI CTGGAG 3 cut(s) 249, 1062, 1684
Bpu10I CCTNAGC 2 cut(s) 1441, 1662
Bpu1102I GCTNAGC 1 cut(s) 948
Bpu14I TTCGAA 1 cut(s) 2492
BpuEI CTTGAG 2 cut(s) 532, 2396
BpuMI CCSGG 4 cut(s) 1794, 2006, 2158, 2159
Bsa29I ATCGAT 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 1578, 1715
BsaI GGTCTC 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 1863, 2055
BsaXI ACNNNNNCTCC 8 cut(s) 1516, 1546, 1548, 1578, 1820, 1850, 2089, 2119
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 1805, 2076, 2163, 2218, 2989
Bse118I RCCGGY 5 cut(s) 1612, 1720, 1863, 2009, 2055
Bse1I ACTGG 5 cut(s) 1079, 1701, 1902, 2184, 2864
Bse3DI GCAATG 8 cut(s) 123, 232, 258, 1070, 1127, 1872, 2745, 2850
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 6 cut(s) 57, 753, 966, 1635, 1751, 2299
BseCI ATCGAT 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 6 cut(s) 86, 1805, 2076, 2163, 2218, 2989
BseMI GCAATG 8 cut(s) 123, 232, 258, 1070, 1127, 1872, 2745, 2850
BseMII CTCAG 6 cut(s) 288, 538, 913, 1653, 1923, 2797
BseNI ACTGG 5 cut(s) 1079, 1701, 1902, 2184, 2864
BseRI GAGGAG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 2621
BseX3I CGGCCG 1 cut(s) 2007
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 3 cut(s) 1013, 1685, 2843
Bsh1285I CGRYCG 2 cut(s) 1521, 2010
BshFI GGCC 6 cut(s) 678, 1248, 1638, 2009, 2840, 2993
BshNI GGYRCC 5 cut(s) 1615, 1714, 2066, 2616, 2747
BshTI ACCGGT 2 cut(s) 1863, 2055
BshVI ATCGAT 1 cut(s) 583
BsiEI CGRYCG 2 cut(s) 1521, 2010
BsiHKAI GWGCWC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 2157
BsiSI CCGG 8 cut(s) 1613, 1721, 1794, 1864, 2006, 2010, 2056, 2158
BslFI GGGAC 3 cut(s) 716, 723, 1514
BslI CCNNNNNNNGG 6 cut(s) 86, 1805, 2076, 2163, 2218, 2989
BsmAI GTCTC 6 cut(s) 222, 1840, 2320, 2448, 2710, 2781
BsmFI GGGAC 3 cut(s) 716, 723, 1514
BsnI GGCC 6 cut(s) 678, 1248, 1638, 2009, 2840, 2993
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 2157
Bsp119I TTCGAA 1 cut(s) 2492
Bsp1286I GDGCHC 3 cut(s) 376, 1902, 2621
Bsp1720I GCTNAGC 1 cut(s) 948
Bsp19I CCATGG 1 cut(s) 564
BspANI GGCC 6 cut(s) 678, 1248, 1638, 2009, 2840, 2993
BspCNI CTCAG 6 cut(s) 287, 537, 912, 1654, 1924, 2796
BspDI ATCGAT 1 cut(s) 583
BspFNI CGCG 3 cut(s) 1013, 1685, 2843
BspHI TCATGA 3 cut(s) 456, 1336, 2872
BspLI GGNNCC 9 cut(s) 797, 1395, 1617, 1639, 1716, 1991, 2068, 2618, 2749
BspMI ACCTGC 1 cut(s) 1567
BspPI GGATC 5 cut(s) 499, 638, 1004, 1132, 1546
BspT104I TTCGAA 1 cut(s) 2492
BspT107I GGYRCC 5 cut(s) 1615, 1714, 2066, 2616, 2747
BspTNI GGTCTC 1 cut(s) 222
BsrBI CCGCTC 1 cut(s) 2077
BsrDI GCAATG 8 cut(s) 123, 232, 258, 1070, 1127, 1872, 2745, 2850
BsrFI RCCGGY 5 cut(s) 1612, 1720, 1863, 2009, 2055
BsrI ACTGG 5 cut(s) 1079, 1701, 1902, 2184, 2864
BssAI RCCGGY 5 cut(s) 1612, 1720, 1863, 2009, 2055
BssNI GRCGYC 2 cut(s) 1578, 1715
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 5 cut(s) 564, 2145, 2449, 2470, 2994
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 6 cut(s) 57, 753, 966, 1635, 1751, 2299
Bst4CI ACNGT 5 cut(s) 431, 698, 1528, 1539, 2704
Bst6I CTCTTC 3 cut(s) 1208, 1506, 1695
BstACI GRCGYC 2 cut(s) 1578, 1715
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstBI TTCGAA 1 cut(s) 2492
BstC8I GCNNGC 5 cut(s) 114, 1320, 1857, 1965, 2752
BstDSI CCRYGG 2 cut(s) 564, 1710
BstEII GGTNACC 1 cut(s) 755
BstENI CCTNNNNNAGG 2 cut(s) 2216, 2987
BstFNI CGCG 3 cut(s) 1013, 1685, 2843
BstH2I RGCGCY 2 cut(s) 1718, 2565
BstHHI GCGC 7 cut(s) 874, 1013, 1564, 1687, 1717, 2564, 2845
BstMAI GTCTC 6 cut(s) 222, 1840, 2320, 2448, 2710, 2781
BstMCI CGRYCG 2 cut(s) 1521, 2010
BstNI CCWGG 6 cut(s) 57, 753, 966, 1635, 1751, 2299
BstNSI RCATGY 1 cut(s) 938
BstPI GGTNACC 1 cut(s) 755
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 2621
BstUI CGCG 3 cut(s) 1013, 1685, 2843
BstV2I GAAGAC 3 cut(s) 323, 348, 1020
BstX2I RGATCY 2 cut(s) 270, 491
BstXI CCANNNNNNTGG 1 cut(s) 1979
BstYI RGATCY 2 cut(s) 270, 491
BstZI CGGCCG 1 cut(s) 2007
Bsu15I ATCGAT 1 cut(s) 583
BsuRI GGCC 6 cut(s) 678, 1248, 1638, 2009, 2840, 2993
BsuTUI ATCGAT 1 cut(s) 583
BtgI CCRYGG 2 cut(s) 564, 1710
BtgZI GCGATG 3 cut(s) 596, 1593, 1961
BtrI CACGTC 1 cut(s) 1368
BtsI GCAGTG 2 cut(s) 948, 2109
BtsIMutI CAGTG 8 cut(s) 80, 436, 532, 703, 907, 948, 1425, 2109
BveI ACCTGC 1 cut(s) 1567
Cac8I GCNNGC 5 cut(s) 114, 1320, 1857, 1965, 2752
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 3 cut(s) 456, 1336, 2872
CfoI GCGC 7 cut(s) 874, 1013, 1564, 1687, 1717, 2564, 2845
Cfr10I RCCGGY 5 cut(s) 1612, 1720, 1863, 2009, 2055
Cfr13I GGNCC 6 cut(s) 235, 1018, 1394, 1637, 1796, 1989
Cfr9I CCCGGG 1 cut(s) 2157
ClaI ATCGAT 1 cut(s) 583
CseI GACGC 3 cut(s) 342, 644, 1586
CsiI ACCWGGT 2 cut(s) 751, 964
Csp6I GTAC 5 cut(s) 363, 1983, 2067, 2080, 2129
CspAI ACCGGT 2 cut(s) 1863, 2055
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 5 cut(s) 363, 1983, 2067, 2080, 2129
DinI GGCGCC 1 cut(s) 1716
DriI GACNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 2007
EagI CGGCCG 1 cut(s) 2007
Eam1104I CTCTTC 3 cut(s) 1208, 1506, 1695
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 3 cut(s) 1208, 1506, 1695
EciI GGCGGA 1 cut(s) 2990
EclXI CGGCCG 1 cut(s) 2007
Eco130I CCWWGG 5 cut(s) 564, 2145, 2449, 2470, 2994
Eco147I AGGCCT 1 cut(s) 2993
Eco24I GRGCYC 1 cut(s) 1902
Eco31I GGTCTC 1 cut(s) 222
Eco32I GATATC 4 cut(s) 746, 855, 2777, 3046
Eco47I GGWCC 5 cut(s) 235, 1018, 1394, 1796, 1989
Eco52I CGGCCG 1 cut(s) 2007
Eco57I CTGAAG 4 cut(s) 456, 2618, 2700, 2742
Eco88I CYCGRG 1 cut(s) 2157
Eco91I GGTNACC 1 cut(s) 755
EcoNI CCTNNNNNAGG 2 cut(s) 2216, 2987
EcoO65I GGTNACC 1 cut(s) 755
EcoRII CCWGG 6 cut(s) 55, 751, 964, 1633, 1749, 2297
EcoRV GATATC 4 cut(s) 746, 855, 2777, 3046
EcoT14I CCWWGG 5 cut(s) 564, 2145, 2449, 2470, 2994
EcoT38I GRGCYC 1 cut(s) 1902
EgeI GGCGCC 1 cut(s) 1716
EheI GGCGCC 1 cut(s) 1716
ErhI CCWWGG 5 cut(s) 564, 2145, 2449, 2470, 2994
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 1268, 1300
FaqI GGGAC 3 cut(s) 716, 723, 1514
FauI CCCGC 6 cut(s) 1327, 1612, 1633, 1659, 1690, 2821
FauNDI CATATG 1 cut(s) 1023
FriOI GRGCYC 1 cut(s) 1902
FspBI CTAG 4 cut(s) 1092, 1268, 1404, 2628
GlaI GCGC 7 cut(s) 873, 1012, 1563, 1686, 1716, 2563, 2844
GsuI CTGGAG 3 cut(s) 249, 1062, 1684
HaeII RGCGCY 2 cut(s) 1718, 2565
HaeIII GGCC 6 cut(s) 678, 1248, 1638, 2009, 2840, 2993
HapII CCGG 8 cut(s) 1613, 1721, 1794, 1864, 2006, 2010, 2056, 2158
HgaI GACGC 3 cut(s) 342, 644, 1586
HhaI GCGC 7 cut(s) 874, 1013, 1564, 1687, 1717, 2564, 2845
Hin1I GRCGYC 2 cut(s) 1578, 1715
Hin6I GCGC 7 cut(s) 872, 1011, 1562, 1685, 1715, 2562, 2843
HinP1I GCGC 7 cut(s) 872, 1011, 1562, 1685, 1715, 2562, 2843
HincII GTYRAC 1 cut(s) 2575
HindII GTYRAC 1 cut(s) 2575
HindIII AAGCTT 2 cut(s) 392, 483
HinfI GANTC 7 cut(s) 422, 460, 545, 1496, 2390, 2860, 3022
HpaII CCGG 8 cut(s) 1613, 1721, 1794, 1864, 2006, 2010, 2056, 2158
HphI GGTGA 7 cut(s) 929, 1533, 1623, 1671, 1735, 2165, 2311
Hpy166II GTNNAC 3 cut(s) 238, 2138, 2575
Hpy8I GTNNAC 3 cut(s) 238, 2138, 2575
Hpy99I CGWCG 4 cut(s) 660, 1018, 1276, 1698
HpyAV CCTTC 9 cut(s) 189, 461, 480, 1521, 1651, 1773, 2110, 2260, 3023
HpyCH4III ACNGT 5 cut(s) 431, 698, 1528, 1539, 2704
HpyCH4IV ACGT 1 cut(s) 1367
HpySE526I ACGT 1 cut(s) 1367
Hsp92I GRCGYC 2 cut(s) 1578, 1715
HspAI GCGC 7 cut(s) 872, 1011, 1562, 1685, 1715, 2562, 2843
KasI GGCGCC 1 cut(s) 1714
KpnI GGTACC 1 cut(s) 2070
LmnI GCTCC 7 cut(s) 268, 371, 620, 1569, 1703, 1751, 2572
LweI GCATC 7 cut(s) 212, 340, 529, 1616, 1637, 2395, 2536
MabI ACCWGGT 2 cut(s) 751, 964
MaeI CTAG 4 cut(s) 1092, 1268, 1404, 2628
MaeII ACGT 1 cut(s) 1367
MaeIII GTNAC 8 cut(s) 755, 1086, 1207, 1539, 1753, 2166, 2285, 2941
MbiI CCGCTC 1 cut(s) 2077
MfeI CAATTG 2 cut(s) 2022, 2969
MflI RGATCY 2 cut(s) 270, 491
MhlI GDGCHC 3 cut(s) 376, 1902, 2621
Mly113I GGCGCC 1 cut(s) 1715
MlyI GAGTC 3 cut(s) 431, 539, 1490
MmeI TCCRAC 4 cut(s) 125, 1794, 1823, 1851
MroXI GAANNNNTTC 1 cut(s) 2389
MseI TTAA 9 cut(s) 68, 159, 252, 737, 1193, 2442, 2459, 2541, 2762
MslI CAYNNNNRTG 2 cut(s) 525, 2582
MspA1I CMGCKG 1 cut(s) 2720
MspI CCGG 8 cut(s) 1613, 1721, 1794, 1864, 2006, 2010, 2056, 2158
MunI CAATTG 2 cut(s) 2022, 2969
MvaI CCWGG 6 cut(s) 57, 753, 966, 1635, 1751, 2299
MvnI CGCG 3 cut(s) 1013, 1685, 2843
NarI GGCGCC 1 cut(s) 1715
NciI CCSGG 4 cut(s) 1794, 2006, 2158, 2159
NcoI CCATGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 1023
NlaIV GGNNCC 9 cut(s) 797, 1395, 1617, 1639, 1716, 1991, 2068, 2618, 2749
NmuCI GTSAC 3 cut(s) 1539, 1753, 2166
NspI RCATGY 1 cut(s) 938
NspV TTCGAA 1 cut(s) 2492
PagI TCATGA 3 cut(s) 456, 1336, 2872
PaqCI CACCTGC 1 cut(s) 1567
PceI AGGCCT 1 cut(s) 2993
PcsI WCGNNNNNNNCGW 2 cut(s) 1702, 2434
PdmI GAANNNNTTC 1 cut(s) 2389
PfeI GAWTC 4 cut(s) 460, 2390, 2860, 3022
PflFI GACNNNGTC 2 cut(s) 233, 1526
PflMI CCANNNNNTGG 1 cut(s) 86
PinAI ACCGGT 2 cut(s) 1863, 2055
Ple19I CGATCG 1 cut(s) 1521
PleI GAGTC 3 cut(s) 430, 539, 1490
PluTI GGCGCC 1 cut(s) 1718
PpsI GAGTC 3 cut(s) 430, 539, 1490
PshBI ATTAAT 2 cut(s) 252, 737
PsiI TTATAA 1 cut(s) 2043
Psp6I CCWGG 6 cut(s) 55, 751, 964, 1633, 1749, 2297
PspEI GGTNACC 1 cut(s) 755
PspGI CCWGG 6 cut(s) 55, 751, 964, 1633, 1749, 2297
PspN4I GGNNCC 9 cut(s) 797, 1395, 1617, 1639, 1716, 1991, 2068, 2618, 2749
PspPI GGNCC 6 cut(s) 235, 1018, 1394, 1637, 1796, 1989
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 270, 491
PsyI GACNNNGTC 2 cut(s) 233, 1526
PvuI CGATCG 1 cut(s) 1521
PvuII CAGCTG 1 cut(s) 2720
RsaI GTAC 5 cut(s) 364, 1984, 2068, 2081, 2130
RsaNI GTAC 5 cut(s) 363, 1983, 2067, 2080, 2129
RseI CAYNNNNRTG 2 cut(s) 525, 2582
SaqAI TTAA 9 cut(s) 68, 159, 252, 737, 1193, 2442, 2459, 2541, 2762
Sau96I GGNCC 6 cut(s) 235, 1018, 1394, 1637, 1796, 1989
SchI GAGTC 3 cut(s) 431, 539, 1490
SduI GDGCHC 3 cut(s) 376, 1902, 2621
SexAI ACCWGGT 2 cut(s) 751, 964
SfaNI GCATC 7 cut(s) 212, 340, 529, 1616, 1637, 2395, 2536
SfcI CTRYAG 1 cut(s) 427
SfoI GGCGCC 1 cut(s) 1716
SfuI TTCGAA 1 cut(s) 2492
SgrAI CRCCGGYG 2 cut(s) 1612, 1720
SinI GGWCC 5 cut(s) 235, 1018, 1394, 1796, 1989
SmaI CCCGGG 1 cut(s) 2159
SmiMI CAYNNNNRTG 2 cut(s) 525, 2582
SmlI CTYRAG 2 cut(s) 547, 2411
SmoI CTYRAG 2 cut(s) 547, 2411
SseBI AGGCCT 1 cut(s) 2993
SspDI GGCGCC 1 cut(s) 1714
SspI AATATT 3 cut(s) 2465, 2799, 3009
SspMI CTAG 4 cut(s) 1092, 1268, 1404, 2628
StuI AGGCCT 1 cut(s) 2993
StyI CCWWGG 5 cut(s) 564, 2145, 2449, 2470, 2994
TaaI ACNGT 5 cut(s) 431, 698, 1528, 1539, 2704
TaiI ACGT 1 cut(s) 1370
TaqII GACCGA 1 cut(s) 399
TauI GCSGC 4 cut(s) 1011, 1720, 2249, 2843
TfiI GAWTC 4 cut(s) 460, 2390, 2860, 3022
Tru1I TTAA 9 cut(s) 68, 159, 252, 737, 1193, 2442, 2459, 2541, 2762
Tru9I TTAA 9 cut(s) 68, 159, 252, 737, 1193, 2442, 2459, 2541, 2762
TscAI CASTG 8 cut(s) 87, 436, 532, 703, 907, 948, 1432, 2116
TseFI GTSAC 3 cut(s) 1539, 1753, 2166
Tsp45I GTSAC 3 cut(s) 1539, 1753, 2166
TspGWI ACGGA 2 cut(s) 1031, 2426
TspMI CCCGGG 1 cut(s) 2157
TspRI CASTG 8 cut(s) 87, 436, 532, 703, 907, 948, 1432, 2116
Tth111I GACNNNGTC 2 cut(s) 233, 1526
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 5 cut(s) 235, 1018, 1394, 1796, 1989
VspI ATTAAT 2 cut(s) 252, 737
XagI CCTNNNNNAGG 2 cut(s) 2216, 2987
XapI RAATTY 6 cut(s) 808, 922, 978, 1138, 1399, 1727
XceI RCATGY 1 cut(s) 938
XmaI CCCGGG 1 cut(s) 2157
XmnI GAANNNNTTC 1 cut(s) 2389
XspI CTAG 4 cut(s) 1092, 1268, 1404, 2628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.