Rw6G015970

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000071
Physical Location & Seq
Forward (+)
31025643 .. 31029399
3757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G015970.1

Sequence Viewer

Length: 3216 bp
ATGCCAGGGGACGAAGAAAGCAAGGTCGTTCAACACGACAACTCTTCGACTACGCTGAAGATGTCAGAACCTTGGGAAAATCCCAATCATCCTCTGTTCATCCATCATTCTGATCAGCCTGGAGCGGTCCTTGTTGCACAGCCTTTGGTGGAAGACAATTATACCACCTGGGCTCAGTCTATGAACATGGCCCTCACTATCAAAAACAAAAGGGGATTTATTGATGGCACAAACCAACGATCAACCCAGAGAGTTGAAGAACAGACGCAATGGGACCGGTGCAATACTCTGGTTAAAACCTGGTTGCTCGGGTCTATGTCCAAAGAGATATCTAGTAGTGTCATTCACTGCAAGACTGCAAAAGGCATGTGGGAGGAGCTGAAAGAAAGGTTTTCCCACACTAATACGGTACAATTATTCCATATTGAGAATGCTATACATGATTGTGAACAGGGTGCTGGCTCTGTGACTACCTTCTTTACCAAACTCAAAGGCTTATGGGATGAGAAAGATGCCCTTTGCGAGATTCCTCCGTGCACTTGTGAAGCATCCAAGGAAATCAAGGATTATATGGATACACAGAAGACAATGAAATTTTTAATGGGGCTTAATGACAATTTTGCAACAATTCGCAGCAGTGTGGTGAACATGGAGCCCTTCCCCACCGTCAACAAAGCATATTCAATGGCACTTCGCCATGAGAAACAAGCAGAGCTGTCTAGTGGGAAGGCTTTAGTGCAACCAGAAGCAGCAGCATTTGCGGTGAAACGGAGCAATCGTGATTTTGAAGCTGTCGAAGGAGGATATTGTCGAAAAAGAAAGGCTCTCATGGGGGAAGGTCAAGGGAGCTCCAAAGGAAATAATGTGGTCTCATTGCAGGACAACAAAAACAATGCCATCAACTTTCCTTTCTCTCAAGAAGACTGCCAGAAATTCTTAGGTCTCATCAAAGATAAGGCCACTACTACCACTGTCAATCAAGTTGGTAACATCCCCAACTATGAAGAACTCTCAGGTAAAACTTATCGCATGAGTCAAAATGGTAAAGAAATCATATGGATTCTAGATAGTGGAGCTAGTGATCACATATCCGCTGTGCATTCCAATAATTCAAACCATCCACATCAGGATCCTATCTTCATGCCACATAATTCCCACCATTCACATCATGACCCTATTTTCATGCCCCATAATTCAAACCATCTCCATCATGATCCTCTTTTTATGCCACAAAATAATCCACCGTACTTGGACCATAAAGAGCCACCAACCACACATGACATTACCCAAAATACCCCTCCTGAGAATCCTCCACTTGACCAACGCATTCCAGCTCCTTCTTCCTCTGATGTCATGCTCCCTTCATCACCAGAACAAAGTCATTCATCATCACCACTAAATCCGGTCCCCAATCAAAATTCCATTCCATCTCTTCCTCAACCACGGCGCTCCACCAGGGCCACCACACAACCATCCTTTCTGCAAGATTTCCACATCGAGGCAGCTCTTCCCTCTCGACCTGCACCATCGTCCTCACCGGCCGAGGTCCGTCACCAAGGTACTCCTCATTCTCTCTCCCATGTTTTATCTTATGATAGGCTTTCTCCCTCTCATCGTGCTTTTGTCACTCAGCTCACTATCGAAAAAGAACCCACTTCCTTTTCACAGGCAATGCAACACCCCAAATGGAGAGAGGCGATGCAAACAGAAATCCAAGCGTTAGAGGCTAATCGGACCTGGACCTTGGTCCCACTTCCTTCACACAAACGACCCATTGGATGCAAATGGGTTTACAAGATTAAGCTCAATCCTGATGGTTCAATTGAACGGTATAAAGCACGATTGGTCGCCAAAGGTTATAGTCAGGTTGAAGGAGTGGATTACCGAGAGACTTTTGCTCCCGTTGCCAAGTTAACCACCGTCCGAGTTCTTCTCAGTCTTGCGGCTATGAAAGGATGGCATCTACATCAATTGGATGTCAACAATGCATTTTTGAATGGTGACCTTTATGAAGAGGTGTATATGCATCTACCTCCTGGTTTCGGACGAAAGGGGGAGAAACGAGTTTGCAGGTTGCATAAGTCAATTTACGGCCTGAAACAAGCCTCACGGCAGTGGTTTATCAAGCTTTCAAGTGCTCTCAAGTCAGCTGGATTTCAACAGTCTTGGTCTGATTATTCGTTATTCGTCCGAAACAGTCAAGGAAAATTCACGGCACTGCTGGTATACGTGGATGATGTGATTCTAGCTGGGAATAGCTTGGAGGATCTTTTGCATACCAAACAATTCCTTTCTAATTGTTTCAAATTGAAAGATATGGGAAAGCTCAAGTATTTCTTGGGTATAGAAGTAGCAAGATCACATAAAGGAATTGTGTTGTGCCAGAGAAAATATGCTCTGGAGATTTTGGAAGATGCAGGCTTCTTAGGTGCAAAGTCTTCTCGGTTTCCTGTAGAACAAGGTTTATCACTCACCCAATCTGATGGCAGTTTATTAGATAATCCTTCTCAATATCGCAGGTTAGTGGGGAGGCTCATATATCTAACAATTACGAGACCGGATATAGTTTATGCTGTTCACATATTAAGCCAATTTATGGATAAGCCACGGCAACCACATCTCGAGGCAGCACATAAGGTCTTAAGATACATTAAACAAGCTCCAGGGCAAGGGCTTCTCTTGCCTTCAACAGGCTCACTGGAGTTAAGAGCATTCTGTGACGCAGACTGGGCACGGTGCAGAGACACAAGGAGATCAGTGTCAGGTTATTGTGTGCTTTTCGGTAATGCAGCTGTCTCATGGAAAACAAAAAAGCAGAGCACTGTAGCACGCTCAAGTGCTGAAGCCGAGTATCGATCCATGGCCACGGCTTGTTGTGAGGTAACTTGGTTACGCAATATATTGCGTGATTTGAATATACATCATAAAGAACCAGTTAAATTGTACTGTGATAATCTTGCTGCAATTCACATTGCTTCAAACCCTGTGTTTCATGAGCGCACAAAGCACATAGAAATTGACTGCCATGTAGTGCGCGAAAAGGTGCAACGAGGCGTTATTAAGACGGCTCACATTCGAACCAAAGATCAGCCGGCCGACTTCTTTACTAAACCAGTAAGTGCTGCACAGTTCAATATGTTGCTTGGCAAGTTGGGTGTAATCAATATACACTCCAACTTGAGGGGGAGTATTGAGGAACAAGATTCTGTGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

1071

Amino Acids

120.63

Weight (kDa)

8.8

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 35 - 77 1.9e-15 gag-polypeptide of LTR copia-type
RVT_2 PF07727 577 - 819 8.7e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1528, 2061, 2508
AccB7I CCANNNNNTGG 1 cut(s) 2596
AccBSI CCGCTC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 2226
AccII CGCG 1 cut(s) 3036
AciI CCGC 4 cut(s) 125, 761, 1092, 1943
AclWI GGATC 5 cut(s) 1124, 1137, 1208, 2274, 2850
AcoI YGGCCR 3 cut(s) 1539, 2862, 3093
AcsI RAATTY 4 cut(s) 593, 932, 1417, 2207
AcuI CTGAAG 2 cut(s) 77, 2862
AfaI GTAC 4 cut(s) 411, 1247, 1561, 2945
AfiI CCNNNNNNNGG 6 cut(s) 1498, 2042, 2596, 2669, 2690, 3180
AflII CTTAAG 1 cut(s) 2641
AgeI ACCGGT 1 cut(s) 276
AjnI CCWGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
AleI CACNNNNGTG 1 cut(s) 2113
Alw21I GWGCWC 4 cut(s) 539, 851, 2140, 2822
Alw26I GTCTC 6 cut(s) 874, 947, 1883, 2548, 2736, 2800
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 5 cut(s) 1124, 1137, 1208, 2274, 2850
Ama87I CYCGRG 2 cut(s) 308, 2621
AoxI GGCC 7 cut(s) 189, 957, 1458, 1539, 2092, 2862, 3093
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 8 cut(s) 633, 749, 752, 1502, 2627, 2789, 2960, 3122
ApoI RAATTY 4 cut(s) 593, 932, 1417, 2207
AsiGI ACCGGT 1 cut(s) 276
AspLEI GCGC 3 cut(s) 1449, 3000, 3036
AsuHPI GGTGA 8 cut(s) 655, 775, 1359, 1383, 1527, 1544, 2012, 2464
AsuII TTCGAA 1 cut(s) 3076
AvaI CYCGRG 2 cut(s) 308, 2621
AvaII GGWCC 8 cut(s) 127, 274, 1252, 1405, 1546, 1734, 1740, 1747
BaeGI GKGCMC 2 cut(s) 539, 2734
BalI TGGCCA 1 cut(s) 2864
BamHI GGATCC 1 cut(s) 1129
BanII GRGCYC 3 cut(s) 175, 657, 851
BbsI GAAGAC 4 cut(s) 159, 590, 927, 2430
Bbv12I GWGCWC 4 cut(s) 539, 851, 2140, 2822
BbvI GCAGC 8 cut(s) 645, 761, 764, 1514, 2639, 2801, 2947, 3109
BceAI ACGGC 7 cut(s) 1460, 2107, 2126, 2229, 2624, 2883, 3081
BciT130I CCWGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
BciVI GTATCC 1 cut(s) 568
BclI TGATCA 2 cut(s) 112, 1081
BcoDI GTCTC 6 cut(s) 874, 947, 1883, 2548, 2736, 2800
BfaI CTAG 5 cut(s) 333, 720, 1064, 1077, 2246
BfmI CTRYAG 2 cut(s) 2451, 2823
BfoI RGCGCY 1 cut(s) 1450
BfrI CTTAAG 1 cut(s) 2641
BfuAI ACCTGC 3 cut(s) 1528, 2061, 2508
BfuI GTATCC 1 cut(s) 568
BisI GCNGC 9 cut(s) 634, 750, 753, 1503, 1944, 2628, 2790, 2961, 3123
BlsI GCNGC 9 cut(s) 635, 751, 754, 1504, 1945, 2629, 2791, 2962, 3124
Bme1390I CCNGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
Bme18I GGWCC 8 cut(s) 127, 274, 1252, 1405, 1546, 1734, 1740, 1747
BmeT110I CYCGRG 2 cut(s) 308, 2621
BmiI GGNNCC 5 cut(s) 275, 654, 1131, 1407, 1749
BmrFI CCNGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
BmrI ACTGGG 1 cut(s) 2737
BmsI GCATC 7 cut(s) 502, 557, 1689, 1769, 1969, 2035, 2404
BmuI ACTGGG 1 cut(s) 2737
BoxI GACNNNNGTC 1 cut(s) 1745
BpiI GAAGAC 4 cut(s) 159, 590, 927, 2430
BplI GAGNNNNNCTC 2 cut(s) 1917, 1949
BpmI CTGGAG 4 cut(s) 141, 2420, 2646, 2720
Bpu14I TTCGAA 1 cut(s) 3076
BpuEI CTTGAG 5 cut(s) 900, 2126, 2312, 2818, 3199
Bsa29I ATCGAT 1 cut(s) 2854
BsaAI YACGTR 1 cut(s) 2230
BsaI GGTCTC 3 cut(s) 874, 947, 2548
BsaWI WCCGGW 3 cut(s) 276, 1402, 2557
BsaXI ACNNNNNCTCC 4 cut(s) 763, 793, 1882, 1912
Bsc4I CCNNNNNNNGG 6 cut(s) 1498, 2042, 2596, 2669, 2690, 3180
Bse118I RCCGGY 3 cut(s) 276, 1537, 3091
Bse1I ACTGG 4 cut(s) 2703, 2732, 2933, 3113
Bse3DI GCAATG 4 cut(s) 275, 872, 1677, 2970
BseBI CCWGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
BseCI ATCGAT 1 cut(s) 2854
BseLI CCNNNNNNNGG 6 cut(s) 1498, 2042, 2596, 2669, 2690, 3180
BseMI GCAATG 4 cut(s) 275, 872, 1677, 2970
BseMII CTCAG 5 cut(s) 188, 1026, 1293, 1643, 1948
BseNI ACTGG 4 cut(s) 2703, 2732, 2933, 3113
BseRI GAGGAG 2 cut(s) 389, 1554
BseSI GKGCMC 2 cut(s) 539, 2734
BseX3I CGGCCG 2 cut(s) 1539, 3093
BseXI GCAGC 8 cut(s) 645, 761, 764, 1514, 2639, 2801, 2947, 3109
BseYI CCCAGC 1 cut(s) 2249
BsgI GTGCAG 3 cut(s) 1506, 2758, 3108
Bsh1236I CGCG 1 cut(s) 3036
Bsh1285I CGRYCG 2 cut(s) 1542, 3096
BshFI GGCC 7 cut(s) 191, 959, 1460, 1541, 2094, 2864, 3095
BshTI ACCGGT 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 2854
BsiEI CGRYCG 2 cut(s) 1542, 3096
BsiHKAI GWGCWC 4 cut(s) 539, 851, 2140, 2822
BsiHKCI CYCGRG 2 cut(s) 308, 2621
BsiSI CCGG 5 cut(s) 277, 1403, 1538, 2558, 3092
BslFI GGGAC 4 cut(s) 23, 287, 1391, 1733
BslI CCNNNNNNNGG 6 cut(s) 1498, 2042, 2596, 2669, 2690, 3180
BsmAI GTCTC 6 cut(s) 874, 947, 1883, 2548, 2736, 2800
BsmFI GGGAC 4 cut(s) 23, 287, 1391, 1733
BsmI GAATGC 4 cut(s) 436, 1099, 1326, 2711
BsnI GGCC 7 cut(s) 191, 959, 1460, 1541, 2094, 2864, 3095
Bso31I GGTCTC 3 cut(s) 874, 947, 2548
BsoBI CYCGRG 2 cut(s) 308, 2621
Bsp119I TTCGAA 1 cut(s) 3076
Bsp1286I GDGCHC 7 cut(s) 175, 539, 657, 851, 2140, 2734, 2822
Bsp19I CCATGG 1 cut(s) 2859
BspACI CCGC 4 cut(s) 125, 761, 1092, 1943
BspANI GGCC 7 cut(s) 191, 959, 1460, 1541, 2094, 2864, 3095
BspCNI CTCAG 5 cut(s) 187, 1025, 1294, 1642, 1947
BspDI ATCGAT 1 cut(s) 2854
BspFNI CGCG 1 cut(s) 3036
BspHI TCATGA 3 cut(s) 1168, 1210, 2992
BspLI GGNNCC 5 cut(s) 275, 654, 1131, 1407, 1749
BspMI ACCTGC 3 cut(s) 1528, 2061, 2508
BspPI GGATC 5 cut(s) 1124, 1137, 1208, 2274, 2850
BspQI GCTCTTC 1 cut(s) 1512
BspT104I TTCGAA 1 cut(s) 3076
BspTI CTTAAG 1 cut(s) 2641
BspTNI GGTCTC 3 cut(s) 874, 947, 2548
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 4 cut(s) 275, 872, 1677, 2970
BsrFI RCCGGY 3 cut(s) 276, 1537, 3091
BsrI ACTGG 4 cut(s) 2703, 2732, 2933, 3113
BssAI RCCGGY 3 cut(s) 276, 1537, 3091
BssNAI GTATAC 1 cut(s) 2227
BssT1I CCWWGG 5 cut(s) 71, 552, 1555, 1743, 2859
Bst1107I GTATAC 1 cut(s) 2227
Bst2UI CCWGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
Bst6I CTCTTC 4 cut(s) 49, 1437, 1512, 2007
BstAFI CTTAAG 1 cut(s) 2641
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 2230
BstBI TTCGAA 1 cut(s) 3076
BstC8I GCNNGC 4 cut(s) 460, 2419, 2830, 3093
BstDEI CTNAG 7 cut(s) 174, 937, 1012, 1302, 1629, 1934, 2425
BstDSI CCRYGG 4 cut(s) 1442, 2606, 2859, 2865
BstEII GGTNACC 1 cut(s) 2000
BstENI CCTNNNNNAGG 1 cut(s) 2688
BstFNI CGCG 1 cut(s) 3036
BstH2I RGCGCY 1 cut(s) 1450
BstHHI GCGC 3 cut(s) 1449, 3000, 3036
BstMAI GTCTC 6 cut(s) 874, 947, 1883, 2548, 2736, 2800
BstMCI CGRYCG 2 cut(s) 1542, 3096
BstMWI GCNNNNNNNGC 6 cut(s) 758, 1724, 1904, 2680, 2729, 3004
BstNI CCWGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
BstNSI RCATGY 1 cut(s) 370
BstPAI GACNNNNGTC 1 cut(s) 1745
BstPI GGTNACC 1 cut(s) 2000
BstSCI CCNGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
BstSFI CTRYAG 2 cut(s) 2451, 2823
BstSLI GKGCMC 2 cut(s) 539, 2734
BstUI CGCG 1 cut(s) 3036
BstV1I GCAGC 8 cut(s) 645, 761, 764, 1514, 2639, 2801, 2947, 3109
BstV2I GAAGAC 4 cut(s) 159, 590, 927, 2430
BstX2I RGATCY 2 cut(s) 1129, 2266
BstXI CCANNNNNNTGG 1 cut(s) 2483
BstYI RGATCY 2 cut(s) 1129, 2266
BstZ17I GTATAC 1 cut(s) 2227
BstZI CGGCCG 2 cut(s) 1539, 3093
Bsu15I ATCGAT 1 cut(s) 2854
BsuI GTATCC 1 cut(s) 568
BsuRI GGCC 7 cut(s) 191, 959, 1460, 1541, 2094, 2864, 3095
BsuTUI ATCGAT 1 cut(s) 2854
BtgI CCRYGG 4 cut(s) 1442, 2606, 2859, 2865
BtgZI GCGATG 1 cut(s) 1712
BtsI GCAGTG 4 cut(s) 346, 643, 2120, 2216
BtsIMutI CAGTG 8 cut(s) 346, 643, 969, 2120, 2216, 2696, 2763, 2820
BveI ACCTGC 3 cut(s) 1528, 2061, 2508
Cac8I GCNNGC 4 cut(s) 460, 2419, 2830, 3093
CciI TCATGA 3 cut(s) 1168, 1210, 2992
CfoI GCGC 3 cut(s) 1449, 3000, 3036
Cfr10I RCCGGY 3 cut(s) 276, 1537, 3091
ClaI ATCGAT 1 cut(s) 2854
CseI GACGC 2 cut(s) 274, 2729
CsiI ACCWGGT 1 cut(s) 299
Csp6I GTAC 4 cut(s) 410, 1246, 1560, 2944
CspAI ACCGGT 1 cut(s) 276
CspCI CAANNNNNGTGG 2 cut(s) 1230, 1265
CviQI GTAC 4 cut(s) 410, 1246, 1560, 2944
DdeI CTNAG 7 cut(s) 174, 937, 1012, 1302, 1629, 1934, 2425
EaeI YGGCCR 3 cut(s) 1539, 2862, 3093
EagI CGGCCG 2 cut(s) 1539, 3093
Eam1104I CTCTTC 4 cut(s) 49, 1437, 1512, 2007
EarI CTCTTC 4 cut(s) 49, 1437, 1512, 2007
Ecl136II GAGCTC 1 cut(s) 849
EclXI CGGCCG 2 cut(s) 1539, 3093
Eco130I CCWWGG 5 cut(s) 71, 552, 1555, 1743, 2859
Eco24I GRGCYC 3 cut(s) 175, 657, 851
Eco31I GGTCTC 3 cut(s) 874, 947, 2548
Eco32I GATATC 1 cut(s) 330
Eco47I GGWCC 8 cut(s) 127, 274, 1252, 1405, 1546, 1734, 1740, 1747
Eco52I CGGCCG 2 cut(s) 1539, 3093
Eco53kI GAGCTC 1 cut(s) 849
Eco57I CTGAAG 2 cut(s) 77, 2862
Eco88I CYCGRG 2 cut(s) 308, 2621
Eco91I GGTNACC 1 cut(s) 2000
EcoICRI GAGCTC 1 cut(s) 849
EcoNI CCTNNNNNAGG 1 cut(s) 2688
EcoO65I GGTNACC 1 cut(s) 2000
EcoRII CCWGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 5 cut(s) 71, 552, 1555, 1743, 2859
EcoT22I ATGCAT 2 cut(s) 1990, 2028
EcoT38I GRGCYC 3 cut(s) 175, 657, 851
ErhI CCWWGG 5 cut(s) 71, 552, 1555, 1743, 2859
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 2321, 2353
FaqI GGGAC 4 cut(s) 23, 287, 1391, 1733
FauNDI CATATG 1 cut(s) 1055
FbaI TGATCA 2 cut(s) 112, 1081
FblI GTMKAC 1 cut(s) 2226
Fnu4HI GCNGC 9 cut(s) 634, 750, 753, 1503, 1944, 2628, 2790, 2961, 3123
FriOI GRGCYC 3 cut(s) 175, 657, 851
Fsp4HI GCNGC 9 cut(s) 634, 750, 753, 1503, 1944, 2628, 2790, 2961, 3123
FspBI CTAG 5 cut(s) 333, 720, 1064, 1077, 2246
GlaI GCGC 3 cut(s) 1448, 2999, 3035
GluI GCNGC 9 cut(s) 634, 750, 753, 1503, 1944, 2628, 2790, 2961, 3123
GsaI CCCAGC 1 cut(s) 2253
GsuI CTGGAG 4 cut(s) 141, 2420, 2646, 2720
HaeII RGCGCY 1 cut(s) 1450
HaeIII GGCC 7 cut(s) 191, 959, 1460, 1541, 2094, 2864, 3095
HapII CCGG 5 cut(s) 277, 1403, 1538, 2558, 3092
HgaI GACGC 2 cut(s) 274, 2729
HhaI GCGC 3 cut(s) 1449, 3000, 3036
Hin6I GCGC 3 cut(s) 1447, 2998, 3034
HinP1I GCGC 3 cut(s) 1447, 2998, 3034
HincII GTYRAC 3 cut(s) 670, 1914, 1981
HindII GTYRAC 3 cut(s) 670, 1914, 1981
HindIII AAGCTT 1 cut(s) 2126
HinfI GANTC 6 cut(s) 526, 1033, 1060, 1306, 2242, 3203
HpaI GTTAAC 1 cut(s) 1914
HpaII CCGG 5 cut(s) 277, 1403, 1538, 2558, 3092
HphI GGTGA 8 cut(s) 655, 775, 1359, 1383, 1527, 1544, 2012, 2464
Hpy166II GTNNAC 9 cut(s) 449, 537, 646, 670, 1792, 1914, 1981, 2227, 2578
Hpy188I TCNGA 9 cut(s) 67, 112, 1348, 1734, 1925, 2045, 2173, 2192, 2482
Hpy8I GTNNAC 9 cut(s) 449, 537, 646, 670, 1792, 1914, 1981, 2227, 2578
HpyCH4IV ACGT 1 cut(s) 2229
HpyF10VI GCNNNNNNNGC 6 cut(s) 758, 1724, 1904, 2680, 2729, 3004
HpyF3I CTNAG 7 cut(s) 174, 937, 1012, 1302, 1629, 1934, 2425
HpySE526I ACGT 1 cut(s) 2229
HspAI GCGC 3 cut(s) 1447, 2998, 3034
KroI GCCGGC 1 cut(s) 3091
KroNI GCCGGC 1 cut(s) 3093
Ksp22I TGATCA 2 cut(s) 112, 1081
KspAI GTTAAC 1 cut(s) 1914
LguI GCTCTTC 1 cut(s) 1512
Lsp1109I GCAGC 8 cut(s) 645, 761, 764, 1514, 2639, 2801, 2947, 3109
LweI GCATC 7 cut(s) 502, 557, 1689, 1769, 1969, 2035, 2404
MabI ACCWGGT 1 cut(s) 299
MaeI CTAG 5 cut(s) 333, 720, 1064, 1077, 2246
MaeII ACGT 1 cut(s) 2229
MaeIII GTNAC 8 cut(s) 466, 986, 1550, 1624, 2000, 2717, 2881, 2889
MbiI CCGCTC 1 cut(s) 125
MfeI CAATTG 2 cut(s) 1821, 1970
MflI RGATCY 2 cut(s) 1129, 2266
MhlI GDGCHC 7 cut(s) 175, 539, 657, 851, 2140, 2734, 2822
MlsI TGGCCA 1 cut(s) 2864
MluNI TGGCCA 1 cut(s) 2864
MlyI GAGTC 1 cut(s) 1042
MmeI TCCRAC 1 cut(s) 3198
Mox20I TGGCCA 1 cut(s) 2864
Mph1103I ATGCAT 2 cut(s) 1990, 2028
MroNI GCCGGC 1 cut(s) 3091
MscI TGGCCA 1 cut(s) 2864
MslI CAYNNNNRTG 2 cut(s) 444, 2113
Msp20I TGGCCA 1 cut(s) 2864
MspA1I CMGCKG 3 cut(s) 1094, 2150, 2792
MspCI CTTAAG 1 cut(s) 2641
MspI CCGG 5 cut(s) 277, 1403, 1538, 2558, 3092
MspR9I CCNGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
MunI CAATTG 2 cut(s) 1821, 1970
Mva1269I GAATGC 4 cut(s) 436, 1099, 1326, 2711
MvaI CCWGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
MvnI CGCG 1 cut(s) 3036
MwoI GCNNNNNNNGC 6 cut(s) 758, 1724, 1904, 2680, 2729, 3004
NaeI GCCGGC 1 cut(s) 3093
NcoI CCATGG 1 cut(s) 2859
NdeI CATATG 1 cut(s) 1055
NgoMIV GCCGGC 1 cut(s) 3091
NlaIV GGNNCC 5 cut(s) 275, 654, 1131, 1407, 1749
NmeAIII GCCGAG 2 cut(s) 1567, 2872
NmuCI GTSAC 5 cut(s) 466, 1550, 1624, 2000, 2717
NsiI ATGCAT 2 cut(s) 1990, 2028
NspI RCATGY 1 cut(s) 370
NspV TTCGAA 1 cut(s) 3076
OliI CACNNNNGTG 1 cut(s) 2113
PaeR7I CTCGAG 1 cut(s) 2621
PagI TCATGA 3 cut(s) 1168, 1210, 2992
PciSI GCTCTTC 1 cut(s) 1512
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 775
PctI GAATGC 4 cut(s) 436, 1099, 1326, 2711
PdiI GCCGGC 1 cut(s) 3093
PfeI GAWTC 5 cut(s) 526, 1060, 1306, 2242, 3203
PflMI CCANNNNNTGG 1 cut(s) 2596
PinAI ACCGGT 1 cut(s) 276
PkrI GCNGC 9 cut(s) 635, 751, 754, 1504, 1945, 2629, 2791, 2962, 3124
PleI GAGTC 1 cut(s) 1041
PpsI GAGTC 1 cut(s) 1041
Ppu21I YACGTR 1 cut(s) 2230
PshAI GACNNNNGTC 1 cut(s) 1745
Psp124BI GAGCTC 1 cut(s) 851
Psp6I CCWGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
PspEI GGTNACC 1 cut(s) 2000
PspFI CCCAGC 1 cut(s) 2249
PspGI CCWGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
PspN4I GGNNCC 5 cut(s) 275, 654, 1131, 1407, 1749
PsuI RGATCY 2 cut(s) 1129, 2266
PvuII CAGCTG 2 cut(s) 2150, 2792
RsaI GTAC 4 cut(s) 411, 1247, 1561, 2945
RsaNI GTAC 4 cut(s) 410, 1246, 1560, 2944
RseI CAYNNNNRTG 2 cut(s) 444, 2113
SacI GAGCTC 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 1512
SatI GCNGC 9 cut(s) 634, 750, 753, 1503, 1944, 2628, 2790, 2961, 3123
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 8 cut(s) 6, 120, 169, 301, 1456, 1738, 2037, 2664
SduI GDGCHC 7 cut(s) 175, 539, 657, 851, 2140, 2734, 2822
SexAI ACCWGGT 1 cut(s) 299
SfaNI GCATC 7 cut(s) 502, 557, 1689, 1769, 1969, 2035, 2404
SfcI CTRYAG 2 cut(s) 2451, 2823
Sfr274I CTCGAG 1 cut(s) 2621
SfuI TTCGAA 1 cut(s) 3076
SinI GGWCC 8 cut(s) 127, 274, 1252, 1405, 1546, 1734, 1740, 1747
SlaI CTCGAG 1 cut(s) 2621
SmiMI CAYNNNNRTG 2 cut(s) 444, 2113
SmlI CTYRAG 7 cut(s) 915, 2141, 2327, 2621, 2641, 2833, 3178
SmoI CTYRAG 7 cut(s) 915, 2141, 2327, 2621, 2641, 2833, 3178
SsiI CCGC 4 cut(s) 125, 761, 1092, 1943
SspMI CTAG 5 cut(s) 333, 720, 1064, 1077, 2246
SstI GAGCTC 1 cut(s) 851
StyD4I CCNGG 8 cut(s) 4, 118, 167, 299, 1454, 1736, 2035, 2662
StyI CCWWGG 5 cut(s) 71, 552, 1555, 1743, 2859
TaiI ACGT 1 cut(s) 2232
TaqI TCGA 9 cut(s) 47, 795, 811, 1497, 1516, 1641, 2622, 2854, 3076
TatI WGTACW 1 cut(s) 2943
TauI GCSGC 1 cut(s) 1946
TfiI GAWTC 5 cut(s) 526, 1060, 1306, 2242, 3203
TscAI CASTG 8 cut(s) 353, 643, 976, 2120, 2223, 2703, 2763, 2827
TseFI GTSAC 5 cut(s) 466, 1550, 1624, 2000, 2717
TseI GCWGC 8 cut(s) 633, 749, 752, 1502, 2627, 2789, 2960, 3122
Tsp45I GTSAC 5 cut(s) 466, 1550, 1624, 2000, 2717
TspGWI ACGGA 3 cut(s) 522, 784, 1538
TspRI CASTG 8 cut(s) 353, 643, 976, 2120, 2223, 2703, 2763, 2827
Van91I CCANNNNNTGG 1 cut(s) 2596
Vha464I CTTAAG 1 cut(s) 2641
VneI GTGCAC 1 cut(s) 535
VpaK11BI GGWCC 8 cut(s) 127, 274, 1252, 1405, 1546, 1734, 1740, 1747
XagI CCTNNNNNAGG 1 cut(s) 2688
XapI RAATTY 4 cut(s) 593, 932, 1417, 2207
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 2621
XmiI GTMKAC 1 cut(s) 2226
XspI CTAG 5 cut(s) 333, 720, 1064, 1077, 2246
Zsp2I ATGCAT 2 cut(s) 1990, 2028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.