Rw5G015250
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000071
Physical Location & Seq
Forward (+)
18876800 .. 18881281
4482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015250.1

Sequence Viewer

Length: 3156 bp
ATGGCAGACGAACAAAAACTCTCTAACCCCTACTTCCTCCACCATTCAGATCATCCAGGTTTGGTTTTAATTTCCAAACCACTGAATGGAGATAATTATCCAACTTGGTTGCGAGCAATGACTATCGCTCTAAATGCCAAGAATAAGTTAGGCTTTGTTAATGGCACTATCAAGCCTCCTTCGGCAGAAACTCATCCAGATAATTATGATACATGGTCTCGATGCAATGACATGGTCCACTCATGGATTATTAATTCCATTGCTCCAGAGATCTCAGACAAACAACTTTCAGTTTCTGCTTACTACACCAAGTTGAAGACTTTATGGGATGAATTATCCTCCTACACAGATGCGTCTTCTTGTACCTGTGGAGCACAGCATGACCGACAAAAGCTTATGCAATTTCTAATGGGTTTGAATGAGTCCTACAGTGCTGCACGAGGACAGATCCTTCTCATGAATCCTCTTCCTTCAGTTAGACAAGCTTATGGATCTATTTGCCAAGTTGAAAAACAAAGGCATCTCAGTGCGACACATGCCACAGACTCAAGCACAAGTGCTGCCATGGCTGTCAGGAACAATCGATCAAATCAGTCTGCTCGACCAACACATCGCTCCTCTACCATTGATGGACGACATATTGATCCAGACCAGCGTCCAGACCAGCGTCGTTTGGGCTCTTCCAAAGGTCGACCTAAATGTTCTTACTGTGGGGACATGGGACACTTTGTTGAAACTTGCTACAAATTAATTGGATATCCACCTGGTCACCCAAAAGAGAGAACAAATCGTTCTATGACTGATGGGAACCGAGGAAAAAATTCAGCAGCCAATCATGTCTCTGAGGGATTAACTAAAGATGACATCAAGTCTATGATATCAGGTGTTTCAGAGGCGCAACTTCAACAACTCTTGTCCATCCTCACACATGCAGCAGTGGCTAAGCCAAAAACCCCACCAGGTTTGTCTAAAATTTCCTCTTGTCGCTGGATCATTGATAGCGGCGCGACGGACCATATGTCTTCATCCTCAAAATTATTTTTACATGGGGGGAAAAGTTGTTCATTGCCTCCAGTATTGTTACCTAGTGGGGAGAAAGCCCATATTGCTGCCAAAGGATCATTGCCTCTAAATTCTACATATTATTTGCATGATGTGCTATGTGTGCCTACATTCAAAGTCGATTTAATGTCCGATCTGGTTACGAAGAGGATGATTGGTTTGGGTAGGCAGCGTAATGGCCTATATTACTTGGTGGCACTAGCGACGAAGAAAACCTTGACCAACCCTCATTCTACCTCAAACAACCTGCCCGCTTGTAACCTCACCACCTCATCTACCGATCTCTGGCATAGTCGCTTAGGACATGCTTATAAATTGTTTGATCTATCTACCCGCAGAATTTTCACCAGTAGAGATGTCGTTTTTCATGAAACATCCTTTCCTTATGCTTCTATCACGTCCAATACCAATTCTTCTTCTTTGGGCCAAAATTCTAGCCCCATTCCTTTCCCCCTCACTGACCCATCTCCCTTAGCTGACCCTACCCATCAGTCTGACCCCATATCTCCCATTGACCCCAACATTGACTCTCTTCTTCCTCCTTCTTCGACCGACACAGTCCCCCCACAGTCACCAACTGCGATCCCACCTGCGCTCCCATCCTCTGACGCCACCGAAAGCCCACCCATATCTCCCGCATCGCCGGTGCCACCCCCCGCATCACCTGGCCCCTTCCCAGACCCGCCTCTGCCTGAGCCTGAACCACCCTTACCCGCGCCTCTTCGTCGCTCTAGTCGTGCCACGGCGCCGCTGGTGAAATTCCGAGATTATGTCTGCTCCCAGGTCTCGCTGCCTTCCTCCGACCCGTCATCTTCTTTGTTTCCCGGTCCAACTAAAGGAACTCGTTATCCCCTCTCCAACTATGTCTCCTACCACCGCTACAAGCTCGCCCACCGGTCTTTCATTGCCCAAATCAGTCAAGCAATCGAGCCCAGTTCCTATTCCGAAGCGGTTGTTCATCCTGAGTGGCAGGAGGCGATAAGAGCTGAATTGCAAGCTCTCCAAGCAAATGGTACATGGACCCTCACTTCTCTCCCGGCCGGTAAGAAGCCAATTGATTGTCGTTGGGTTTATAAAATCAAACACCGGTCTGATGGTACCATTGAGCGGTACAAAGCCCGATTAGAAACCTTCTCTCCCACTGCCAAAATTATTTCTGTACGATGTTTACTTGCCTTGGCAGCAGCCCGGGGATGGTCACTTCACCAACTGGATGTCAATAACGCTTTCTTACATGGCGACCTCCATGAGGAAATCTATATGAAAGATTGGTCTGCCGCCTTCACAAGTCATTATATGGCTATTCGATCTGCTGGTTACACACAATCCAGGGCTGACTACTCTTTATTCACCAAGAGACAAGGCAAGTCTTTTACTGCCCTCTTGATATATGTTGATGATATTTTGATTACGGGAAATGATTCTGCAAGTATTGATGCACTCAAGCAATTTCTTCATCGTAATTTCCGTATTAAAGACCTTGGAGACTTAAAATATTTCCTTGGCATTGAGGTTTCTGCTTCGAAAAATGGGATTTTTATTTCTCAACGAAAATATGCGTTAGAGATTATTAAGGATGCAGGGTTGATGGGCGCTGCTCCTATTGACACACCTATGGAACGAGGACTGAAGTTATCTGACATGAGGTGCCCCATTACTAGAAGATCAACCACAGGTTATTGTGTTTTTCTTGGTTCTTCGTTGGTTTCATGGAGATCAAAAAGACAGAAAACAGTCTCGCTTTCTTCAGCTGAAGCGGAGTATCGTGCAATGACAGGTGCCTGCTGTGAATTAACATGGCTTCGATATCTACTCAGAGACTTAGGACTATTACATCCAGAACCCGCCTTGCTATTTTGTGATAACAAGGCCGCGCTACACATTGCAGCGAATCCAGTTTTTCATGAGCGAACTCGTCATATTGAAATGGACTGCCACTACATCAGGGATAAAATCCAAGATGGGTCTGTTGTTACTAAGTTTGTCATTTCTGCTCATCAATTGGCGGATGTCCTCACTAAGGCCTTGGGAAAGGAAATATTCCTTCCTATGATTCGCAAGTTGGGAGTTCGAGATATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

1051

Amino Acids

116.0

Weight (kDa)

9.12

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 14 - 60 4.7e-21 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 330 - 398 1.7e-07 Pol polyprotein, beta-barrel domain
SH3_retrovirus PF25597 456 - 486 1.5e-07 Retroviral polymerase SH3-like domain
RVT_2 PF07727 691 - 777 1.9e-18 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 790 - 894 1.5e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1374, 2136
AarI CACCTGC 1 cut(s) 1660
Acc36I ACCTGC 2 cut(s) 1317, 1660
Acc65I GGTACC 1 cut(s) 2159
AccB1I GGYRCC 5 cut(s) 1708, 1807, 2159, 2709, 2840
AccB7I CCANNNNNTGG 1 cut(s) 86
AccBSI CCGCTC 1 cut(s) 2170
AccI GTMKAC 1 cut(s) 691
AccII CGCG 3 cut(s) 1007, 1778, 2936
AclWI GGATC 6 cut(s) 442, 499, 638, 998, 1126, 1639
AcoI YGGCCR 1 cut(s) 2100
AcsI RAATTY 6 cut(s) 820, 972, 1132, 1401, 1492, 1820
AcuI CTGAAG 4 cut(s) 456, 2711, 2793, 2835
AcyI GRCGYC 2 cut(s) 1671, 1808
AfaI GTAC 5 cut(s) 364, 2077, 2161, 2174, 2223
AfiI CCNNNNNNNGG 7 cut(s) 86, 1347, 1898, 2169, 2256, 2311, 3082
AgeI ACCGGT 2 cut(s) 1956, 2148
AgsI TTSAA 7 cut(s) 316, 418, 509, 734, 905, 1177, 2987
AhdI GACNNNNNGTC 2 cut(s) 868, 1018
AjiI CACGTC 1 cut(s) 1461
AjnI CCWGG 6 cut(s) 55, 763, 958, 1726, 1842, 2390
AloI GAACNNNNNNTCC 4 cut(s) 1045, 1077, 1894, 1926
AluBI AGCT 7 cut(s) 394, 485, 1538, 1948, 2048, 2060, 2813
AluI AGCT 7 cut(s) 394, 485, 1538, 1948, 2048, 2060, 2813
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 8 cut(s) 222, 844, 1852, 1933, 2413, 2541, 2803, 2874
AlwI GGATC 6 cut(s) 442, 499, 638, 998, 1126, 1639
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 2250
AoxI GGCC 6 cut(s) 1240, 1486, 1729, 2100, 2931, 3084
ApoI RAATTY 6 cut(s) 820, 972, 1132, 1401, 1492, 1820
AseI ATTAAT 2 cut(s) 252, 749
AsiGI ACCGGT 2 cut(s) 1956, 2148
Asp700I GAANNNNTTC 2 cut(s) 820, 2482
Asp718I GGTACC 1 cut(s) 2159
AspLEI GCGC 7 cut(s) 898, 1007, 1657, 1780, 1810, 2657, 2938
AspS9I GGNCC 6 cut(s) 235, 1012, 1486, 1730, 1889, 2082
AsuC2I CCSGG 4 cut(s) 1887, 2099, 2251, 2252
AsuHPI GGTGA 8 cut(s) 761, 1318, 1399, 1626, 1716, 1828, 2258, 2404
AsuII TTCGAA 1 cut(s) 2585
AvaI CYCGRG 1 cut(s) 2250
AvaII GGWCC 4 cut(s) 235, 1012, 1889, 2082
BaeGI GKGCMC 1 cut(s) 2714
BanI GGYRCC 5 cut(s) 1708, 1807, 2159, 2709, 2840
BanII GRGCYC 2 cut(s) 680, 1995
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 3 cut(s) 323, 348, 1014
Bbv12I GWGCWC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 1821
BciT130I CCWGG 6 cut(s) 57, 765, 960, 1728, 1844, 2392
BcnI CCSGG 4 cut(s) 1887, 2099, 2251, 2252
BcoDI GTCTC 8 cut(s) 222, 844, 1852, 1933, 2413, 2541, 2803, 2874
BfaI CTAG 5 cut(s) 1086, 1262, 1497, 1794, 2721
BfmI CTRYAG 1 cut(s) 427
BfoI RGCGCY 2 cut(s) 1811, 2658
BfuAI ACCTGC 2 cut(s) 1317, 1660
BglII AGATCT 1 cut(s) 270
BlpI GCTNAGC 1 cut(s) 942
Bme18I GGWCC 4 cut(s) 235, 1012, 1889, 2082
BmeRI GACNNNNNGTC 2 cut(s) 868, 1018
BmeT110I CYCGRG 1 cut(s) 2250
BmgBI CACGTC 1 cut(s) 1461
BmgT120I GGNCC 6 cut(s) 235, 1012, 1486, 1730, 1889, 2082
BmiI GGNNCC 8 cut(s) 809, 1710, 1732, 1809, 2084, 2161, 2711, 2842
BmrI ACTGGG 1 cut(s) 1989
BmsI GCATC 7 cut(s) 212, 340, 529, 1709, 1730, 2488, 2629
BmuI ACTGGG 1 cut(s) 1989
BoxI GACNNNNGTC 2 cut(s) 654, 666
BpiI GAAGAC 3 cut(s) 323, 348, 1014
BpmI CTGGAG 2 cut(s) 249, 1056
Bpu10I CCTNAGC 3 cut(s) 1360, 1534, 1755
Bpu1102I GCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 2585
BpuEI CTTGAG 2 cut(s) 532, 2489
BpuMI CCSGG 4 cut(s) 1887, 2099, 2251, 2252
Bsa29I ATCGAT 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 1671, 1808
BsaI GGTCTC 2 cut(s) 222, 1852
BsaWI WCCGGW 2 cut(s) 1956, 2148
BsaXI ACNNNNNCTCC 6 cut(s) 1641, 1671, 1913, 1943, 2182, 2212
Bsc4I CCNNNNNNNGG 7 cut(s) 86, 1347, 1898, 2169, 2256, 2311, 3082
Bse118I RCCGGY 4 cut(s) 1705, 1956, 2102, 2148
Bse1I ACTGG 5 cut(s) 1073, 1410, 1995, 2277, 2957
Bse3DI GCAATG 8 cut(s) 123, 232, 258, 1064, 1121, 1965, 2838, 2943
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 6 cut(s) 57, 765, 960, 1728, 1844, 2392
BseCI ATCGAT 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 7 cut(s) 86, 1347, 1898, 2169, 2256, 2311, 3082
BseMI GCAATG 8 cut(s) 123, 232, 258, 1064, 1121, 1965, 2838, 2943
BseMII CTCAG 6 cut(s) 288, 538, 834, 1746, 2016, 2890
BseNI ACTGG 5 cut(s) 1073, 1410, 1995, 2277, 2957
BseRI GAGGAG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 2714
BseX3I CGGCCG 1 cut(s) 2100
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 3 cut(s) 1007, 1778, 2936
Bsh1285I CGRYCG 2 cut(s) 1614, 2103
BshFI GGCC 6 cut(s) 1242, 1488, 1731, 2102, 2933, 3086
BshNI GGYRCC 5 cut(s) 1708, 1807, 2159, 2709, 2840
BshTI ACCGGT 2 cut(s) 1956, 2148
BshVI ATCGAT 1 cut(s) 583
BsiEI CGRYCG 2 cut(s) 1614, 2103
BsiHKAI GWGCWC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 2250
BsiSI CCGG 7 cut(s) 1706, 1887, 1957, 2099, 2103, 2149, 2251
BslFI GGGAC 3 cut(s) 728, 735, 1607
BslI CCNNNNNNNGG 7 cut(s) 86, 1347, 1898, 2169, 2256, 2311, 3082
BsmAI GTCTC 8 cut(s) 222, 844, 1852, 1933, 2413, 2541, 2803, 2874
BsmFI GGGAC 3 cut(s) 728, 735, 1607
BsnI GGCC 6 cut(s) 1242, 1488, 1731, 2102, 2933, 3086
Bso31I GGTCTC 2 cut(s) 222, 1852
BsoBI CYCGRG 1 cut(s) 2250
Bsp119I TTCGAA 1 cut(s) 2585
Bsp1286I GDGCHC 4 cut(s) 376, 680, 1995, 2714
Bsp1720I GCTNAGC 1 cut(s) 942
Bsp19I CCATGG 1 cut(s) 564
BspANI GGCC 6 cut(s) 1242, 1488, 1731, 2102, 2933, 3086
BspCNI CTCAG 6 cut(s) 287, 537, 835, 1747, 2017, 2889
BspDI ATCGAT 1 cut(s) 583
BspFNI CGCG 3 cut(s) 1007, 1778, 2936
BspHI TCATGA 3 cut(s) 456, 1429, 2965
BspLI GGNNCC 8 cut(s) 809, 1710, 1732, 1809, 2084, 2161, 2711, 2842
BspMI ACCTGC 2 cut(s) 1317, 1660
BspPI GGATC 6 cut(s) 442, 499, 638, 998, 1126, 1639
BspQI GCTCTTC 1 cut(s) 685
BspT104I TTCGAA 1 cut(s) 2585
BspT107I GGYRCC 5 cut(s) 1708, 1807, 2159, 2709, 2840
BspTNI GGTCTC 2 cut(s) 222, 1852
BsrBI CCGCTC 1 cut(s) 2170
BsrDI GCAATG 8 cut(s) 123, 232, 258, 1064, 1121, 1965, 2838, 2943
BsrFI RCCGGY 4 cut(s) 1705, 1956, 2102, 2148
BsrI ACTGG 5 cut(s) 1073, 1410, 1995, 2277, 2957
BssAI RCCGGY 4 cut(s) 1705, 1956, 2102, 2148
BssNI GRCGYC 2 cut(s) 1671, 1808
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 5 cut(s) 564, 2238, 2542, 2563, 3087
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 6 cut(s) 57, 765, 960, 1728, 1844, 2392
Bst4CI ACNGT 5 cut(s) 431, 710, 1621, 1632, 2797
Bst6I CTCTTC 5 cut(s) 471, 685, 1202, 1599, 1788
BstACI GRCGYC 2 cut(s) 1671, 1808
BstAPI GCANNNNNTGC 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 2585
BstC8I GCNNGC 5 cut(s) 114, 1314, 1950, 2058, 2845
BstDSI CCRYGG 2 cut(s) 564, 1803
BstEII GGTNACC 1 cut(s) 767
BstENI CCTNNNNNAGG 2 cut(s) 2309, 3080
BstFNI CGCG 3 cut(s) 1007, 1778, 2936
BstH2I RGCGCY 2 cut(s) 1811, 2658
BstHHI GCGC 7 cut(s) 898, 1007, 1657, 1780, 1810, 2657, 2938
BstMAI GTCTC 8 cut(s) 222, 844, 1852, 1933, 2413, 2541, 2803, 2874
BstMCI CGRYCG 2 cut(s) 1614, 2103
BstNI CCWGG 6 cut(s) 57, 765, 960, 1728, 1844, 2392
BstNSI RCATGY 3 cut(s) 539, 932, 1370
BstPAI GACNNNNGTC 2 cut(s) 654, 666
BstPI GGTNACC 1 cut(s) 767
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 2714
BstUI CGCG 3 cut(s) 1007, 1778, 2936
BstV2I GAAGAC 3 cut(s) 323, 348, 1014
BstX2I RGATCY 3 cut(s) 270, 447, 491
BstXI CCANNNNNNTGG 1 cut(s) 2072
BstYI RGATCY 3 cut(s) 270, 447, 491
BstZI CGGCCG 1 cut(s) 2100
Bsu15I ATCGAT 1 cut(s) 583
BsuRI GGCC 6 cut(s) 1242, 1488, 1731, 2102, 2933, 3086
BsuTUI ATCGAT 1 cut(s) 583
BtgI CCRYGG 2 cut(s) 564, 1803
BtgZI GCGATG 2 cut(s) 596, 1686
BtrI CACGTC 1 cut(s) 1461
BtsI GCAGTG 2 cut(s) 942, 2202
BtsIMutI CAGTG 6 cut(s) 80, 436, 532, 942, 1518, 2202
BveI ACCTGC 2 cut(s) 1317, 1660
Cac8I GCNNGC 5 cut(s) 114, 1314, 1950, 2058, 2845
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 3 cut(s) 456, 1429, 2965
CfoI GCGC 7 cut(s) 898, 1007, 1657, 1780, 1810, 2657, 2938
Cfr10I RCCGGY 4 cut(s) 1705, 1956, 2102, 2148
Cfr13I GGNCC 6 cut(s) 235, 1012, 1486, 1730, 1889, 2082
Cfr9I CCCGGG 1 cut(s) 2250
ClaI ATCGAT 1 cut(s) 583
CseI GACGC 4 cut(s) 342, 644, 656, 1679
CsiI ACCWGGT 2 cut(s) 763, 958
Csp6I GTAC 5 cut(s) 363, 2076, 2160, 2173, 2222
CspAI ACCGGT 2 cut(s) 1956, 2148
CspCI CAANNNNNGTGG 2 cut(s) 945, 980
CviQI GTAC 5 cut(s) 363, 2076, 2160, 2173, 2222
DinI GGCGCC 1 cut(s) 1809
DriI GACNNNNNGTC 2 cut(s) 868, 1018
EaeI YGGCCR 1 cut(s) 2100
EagI CGGCCG 1 cut(s) 2100
Eam1104I CTCTTC 5 cut(s) 471, 685, 1202, 1599, 1788
Eam1105I GACNNNNNGTC 2 cut(s) 868, 1018
EarI CTCTTC 5 cut(s) 471, 685, 1202, 1599, 1788
EciI GGCGGA 1 cut(s) 3083
EclXI CGGCCG 1 cut(s) 2100
Eco130I CCWWGG 5 cut(s) 564, 2238, 2542, 2563, 3087
Eco147I AGGCCT 1 cut(s) 3086
Eco24I GRGCYC 2 cut(s) 680, 1995
Eco31I GGTCTC 2 cut(s) 222, 1852
Eco32I GATATC 4 cut(s) 758, 879, 2870, 3139
Eco47I GGWCC 4 cut(s) 235, 1012, 1889, 2082
Eco52I CGGCCG 1 cut(s) 2100
Eco57I CTGAAG 4 cut(s) 456, 2711, 2793, 2835
Eco88I CYCGRG 1 cut(s) 2250
Eco91I GGTNACC 1 cut(s) 767
EcoNI CCTNNNNNAGG 2 cut(s) 2309, 3080
EcoO65I GGTNACC 1 cut(s) 767
EcoRII CCWGG 6 cut(s) 55, 763, 958, 1726, 1842, 2390
EcoRV GATATC 4 cut(s) 758, 879, 2870, 3139
EcoT14I CCWWGG 5 cut(s) 564, 2238, 2542, 2563, 3087
EcoT38I GRGCYC 2 cut(s) 680, 1995
EgeI GGCGCC 1 cut(s) 1809
EheI GGCGCC 1 cut(s) 1809
ErhI CCWWGG 5 cut(s) 564, 2238, 2542, 2563, 3087
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 1262, 1294
FaqI GGGAC 3 cut(s) 728, 735, 1607
FauI CCCGC 7 cut(s) 1321, 1403, 1705, 1726, 1752, 1783, 2914
FauNDI CATATG 1 cut(s) 1017
FblI GTMKAC 1 cut(s) 691
FriOI GRGCYC 2 cut(s) 680, 1995
FspBI CTAG 5 cut(s) 1086, 1262, 1497, 1794, 2721
GlaI GCGC 7 cut(s) 897, 1006, 1656, 1779, 1809, 2656, 2937
GsuI CTGGAG 2 cut(s) 249, 1056
HaeII RGCGCY 2 cut(s) 1811, 2658
HaeIII GGCC 6 cut(s) 1242, 1488, 1731, 2102, 2933, 3086
HapII CCGG 7 cut(s) 1706, 1887, 1957, 2099, 2103, 2149, 2251
HgaI GACGC 4 cut(s) 342, 644, 656, 1679
HhaI GCGC 7 cut(s) 898, 1007, 1657, 1780, 1810, 2657, 2938
Hin1I GRCGYC 2 cut(s) 1671, 1808
Hin6I GCGC 7 cut(s) 896, 1005, 1655, 1778, 1808, 2655, 2936
HinP1I GCGC 7 cut(s) 896, 1005, 1655, 1778, 1808, 2655, 2936
HincII GTYRAC 1 cut(s) 692
HindII GTYRAC 1 cut(s) 692
HindIII AAGCTT 2 cut(s) 392, 483
HinfI GANTC 7 cut(s) 422, 460, 545, 1589, 2483, 2953, 3115
HpaII CCGG 7 cut(s) 1706, 1887, 1957, 2099, 2103, 2149, 2251
HphI GGTGA 8 cut(s) 761, 1318, 1399, 1626, 1716, 1828, 2258, 2404
Hpy166II GTNNAC 3 cut(s) 238, 692, 2231
Hpy8I GTNNAC 3 cut(s) 238, 692, 2231
Hpy99I CGWCG 4 cut(s) 672, 1012, 1270, 1791
HpyAV CCTTC 9 cut(s) 189, 461, 480, 1614, 1744, 1866, 2203, 2353, 3116
HpyCH4III ACNGT 5 cut(s) 431, 710, 1621, 1632, 2797
HpyCH4IV ACGT 1 cut(s) 1460
HpySE526I ACGT 1 cut(s) 1460
Hsp92I GRCGYC 2 cut(s) 1671, 1808
HspAI GCGC 7 cut(s) 896, 1005, 1655, 1778, 1808, 2655, 2936
KasI GGCGCC 1 cut(s) 1807
KpnI GGTACC 1 cut(s) 2163
LguI GCTCTTC 1 cut(s) 685
LmnI GCTCC 6 cut(s) 268, 371, 620, 1662, 1844, 2665
LweI GCATC 7 cut(s) 212, 340, 529, 1709, 1730, 2488, 2629
MabI ACCWGGT 2 cut(s) 763, 958
MaeI CTAG 5 cut(s) 1086, 1262, 1497, 1794, 2721
MaeII ACGT 1 cut(s) 1460
MaeIII GTNAC 8 cut(s) 767, 1080, 1201, 1319, 1632, 2259, 2378, 3034
MbiI CCGCTC 1 cut(s) 2170
MfeI CAATTG 2 cut(s) 2115, 3062
MflI RGATCY 3 cut(s) 270, 447, 491
MhlI GDGCHC 4 cut(s) 376, 680, 1995, 2714
Mly113I GGCGCC 1 cut(s) 1808
MlyI GAGTC 3 cut(s) 431, 539, 1583
MmeI TCCRAC 4 cut(s) 125, 1887, 1916, 1944
MroXI GAANNNNTTC 2 cut(s) 820, 2482
MslI CAYNNNNRTG 2 cut(s) 525, 2675
MspA1I CMGCKG 2 cut(s) 1813, 2813
MspI CCGG 7 cut(s) 1706, 1887, 1957, 2099, 2103, 2149, 2251
MunI CAATTG 2 cut(s) 2115, 3062
MvaI CCWGG 6 cut(s) 57, 765, 960, 1728, 1844, 2392
MvnI CGCG 3 cut(s) 1007, 1778, 2936
NarI GGCGCC 1 cut(s) 1808
NciI CCSGG 4 cut(s) 1887, 2099, 2251, 2252
NcoI CCATGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 1017
NlaIV GGNNCC 8 cut(s) 809, 1710, 1732, 1809, 2084, 2161, 2711, 2842
NmuCI GTSAC 3 cut(s) 767, 1632, 2259
NspI RCATGY 3 cut(s) 539, 932, 1370
NspV TTCGAA 1 cut(s) 2585
PagI TCATGA 3 cut(s) 456, 1429, 2965
PaqCI CACCTGC 1 cut(s) 1660
PceI AGGCCT 1 cut(s) 3086
PciSI GCTCTTC 1 cut(s) 685
PcsI WCGNNNNNNNCGW 2 cut(s) 1795, 2527
PdmI GAANNNNTTC 2 cut(s) 820, 2482
PfeI GAWTC 4 cut(s) 460, 2483, 2953, 3115
PflFI GACNNNGTC 2 cut(s) 233, 1619
PflMI CCANNNNNTGG 1 cut(s) 86
PinAI ACCGGT 2 cut(s) 1956, 2148
PleI GAGTC 3 cut(s) 430, 539, 1583
PluTI GGCGCC 1 cut(s) 1811
PpsI GAGTC 3 cut(s) 430, 539, 1583
PshAI GACNNNNGTC 2 cut(s) 654, 666
PshBI ATTAAT 2 cut(s) 252, 749
PsiI TTATAA 2 cut(s) 1374, 2136
Psp6I CCWGG 6 cut(s) 55, 763, 958, 1726, 1842, 2390
PspEI GGTNACC 1 cut(s) 767
PspGI CCWGG 6 cut(s) 55, 763, 958, 1726, 1842, 2390
PspN4I GGNNCC 8 cut(s) 809, 1710, 1732, 1809, 2084, 2161, 2711, 2842
PspPI GGNCC 6 cut(s) 235, 1012, 1486, 1730, 1889, 2082
PsrI GAACNNNNNNTAC 2 cut(s) 1755, 1787
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 3 cut(s) 270, 447, 491
PsyI GACNNNGTC 2 cut(s) 233, 1619
PvuII CAGCTG 1 cut(s) 2813
RsaI GTAC 5 cut(s) 364, 2077, 2161, 2174, 2223
RsaNI GTAC 5 cut(s) 363, 2076, 2160, 2173, 2222
RseI CAYNNNNRTG 2 cut(s) 525, 2675
SalI GTCGAC 1 cut(s) 690
SapI GCTCTTC 1 cut(s) 685
Sau96I GGNCC 6 cut(s) 235, 1012, 1486, 1730, 1889, 2082
SchI GAGTC 3 cut(s) 431, 539, 1583
SduI GDGCHC 4 cut(s) 376, 680, 1995, 2714
SexAI ACCWGGT 2 cut(s) 763, 958
SfaNI GCATC 7 cut(s) 212, 340, 529, 1709, 1730, 2488, 2629
SfcI CTRYAG 1 cut(s) 427
SfoI GGCGCC 1 cut(s) 1809
SfuI TTCGAA 1 cut(s) 2585
SgrAI CRCCGGYG 1 cut(s) 1705
SinI GGWCC 4 cut(s) 235, 1012, 1889, 2082
SmaI CCCGGG 1 cut(s) 2252
SmiMI CAYNNNNRTG 2 cut(s) 525, 2675
SmlI CTYRAG 2 cut(s) 547, 2504
SmoI CTYRAG 2 cut(s) 547, 2504
SseBI AGGCCT 1 cut(s) 3086
SspDI GGCGCC 1 cut(s) 1807
SspI AATATT 2 cut(s) 2558, 3102
SspMI CTAG 5 cut(s) 1086, 1262, 1497, 1794, 2721
StuI AGGCCT 1 cut(s) 3086
StyI CCWWGG 5 cut(s) 564, 2238, 2542, 2563, 3087
TaaI ACNGT 5 cut(s) 431, 710, 1621, 1632, 2797
TaiI ACGT 1 cut(s) 1463
TaqII GACCGA 2 cut(s) 399, 1628
TauI GCSGC 4 cut(s) 1005, 1813, 2342, 2936
TfiI GAWTC 4 cut(s) 460, 2483, 2953, 3115
TscAI CASTG 6 cut(s) 87, 436, 532, 942, 1525, 2209
TseFI GTSAC 3 cut(s) 767, 1632, 2259
Tsp45I GTSAC 3 cut(s) 767, 1632, 2259
TspGWI ACGGA 2 cut(s) 1025, 2519
TspMI CCCGGG 1 cut(s) 2250
TspRI CASTG 6 cut(s) 87, 436, 532, 942, 1525, 2209
Tth111I GACNNNGTC 2 cut(s) 233, 1619
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 4 cut(s) 235, 1012, 1889, 2082
VspI ATTAAT 2 cut(s) 252, 749
XagI CCTNNNNNAGG 2 cut(s) 2309, 3080
XapI RAATTY 6 cut(s) 820, 972, 1132, 1401, 1492, 1820
XceI RCATGY 3 cut(s) 539, 932, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 1810
XmaI CCCGGG 1 cut(s) 2250
XmiI GTMKAC 1 cut(s) 691
XmnI GAANNNNTTC 2 cut(s) 820, 2482
XspI CTAG 5 cut(s) 1086, 1262, 1497, 1794, 2721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.