FvH4_6g36661

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000071
Physical Location & Seq
Reverse (-)
28860712 .. 28863625
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36661.t1

Sequence Viewer

Length: 2097 bp
ATGAGAACGGTGTATCTCCCTCTCCTTGATCTGATCGGCTTGCTTAAGAGCGGCCGTTTCGATCTCGTCGGCTATCTTGGAGGGCTCGGCGGCGAACTCGAGGGATCGGTTGAGATTTTGGCGGAGGTGGTGAACTGGGGATGGCCTGATTTCTTGGCTGCTTCTTCGGCGAAGCTATTGGGTCGTTTCCACAGATCTTCCATTGTTTGTCTCTTCAGTTGCAGGACTGAGGGAGAAAAAGATAATGTGTTGGTGGACACAACACCAGCCGTGGTCCCAGGTGCTGACGCAAGCCAGTTAGCTACTACAGATGAGCCGAGCCGAGCTTCAGATTGTGACACAGGGACCACAGACGAGCCAAGCCAACTGAGCCGAGCCGACCAAGGCGCTGGCCCGTTGCTGACAAATGTCGACAATGACACTGGTCCCGCAGATCAAAACAAATCAGTCTCCTTCTCTGGTCCCACAAACTTGTCAGATAATTGTCCCATAATTGAAGGTTCTGCCTCATCCGATAATTTTCCCCTCTCTACACCGTTAACCGAAGAATCCACCACAAATGTCTCTCCTCAGGATGCAATGGCGGATCCGAAGTGGACTCAAGCGATGAATGAAGAACTAGAAGCCTTGCAAAAGAACTCGACCTGGGATGTTGTTCATAAAACTACTGGAAAAAAGACCGTTGGATGCCGTTGGGTATTTACGGTTAAACTCAAAGCCGATGGGAGCATTGATAGATATAAAGCCAGATTAGTTTCCAAAGGATACACCCAACGCTATGGGATTGATTATGAAGAAACATTTGTGCCTGTAGCAAAGATCAACACTGTTCGAATCTTGATTTCACTTGCAACAACTAAAGATTGGCCTCTACGACAGTTTGATGTGAAGAATGCTTTCCTCAATGGAAACTTGGAAGAAGAGGTGTATATGGACATGCCCCCAGGAGTCAAATGTAGACCTTGCGATGTCGGAAAGGTTTGTAAGTTGAAGAAATCTCTGTATGGTTTGAAGCAGTCTCCTAGAGCTTGGTTTGGAAGATTCTCTAAATCAATGAAGACATTCGGTTATAGACAAAGCAACTCGGATCACACTTTGTTCATCAAACGTAATCAGGGTAAGATCATAGCTCTGATTGTGTATGTTGATGATATGATTGTTACAGGAGATGACCCAAAGGAGATGGAGGCTCTACAGAAGTACCTTTCAAAGGAGTTTGAGATGAAGGACCTTGGACAATTGAAGTATTTTCTCGGAATTGAAGTTGCAAGGTCAAAGGAGGGGATTTCTTTATCCCAGCGAAAGTATGTACTTGACTTGTTAGCTGAGACTGGAATGCTTGACTACAAGCCTGTTGAGACACCAATTGAGATGAATCACAACCTTGCCATCTATCCAGATCAAGTTCCAACTGATAAAAGAAGGTACCAACGTCTAGTAGGGAGACTTATTTATCTTTCTCATACTAGACCTGATATTGCTTATGCTGTGAGTGTGGTTAGTCAATTTATGCATTGTCCCAGCGAAGAGCATATGGATGCAATGTATCGTATTCTGAAGTATCTTAAGATGACACCTGGAAAAGGCTTGTTATTTGAAAAGAGGGGTGAATTGGAAGTTGTAGGGTACACAGATGCTGATTGGGCTGGTGATAAGACTGACAGACGGTCTACATCTGGGTATTTTACATTTGTTGGAGGAAACCTTGTGACTTGGCGTAGTAAGAAACAAAAAGTCGTTGCAAGATCAAGTGCTGAAGCAGAGTTTCGAGGTATGGCACATGGAGTTTGTGAGATGCTATGGATTCGTAATGTATTGAAAGAATTGGGTTTCAAACTCAAGAAACCCATGGACTTGCATTGTGATAGCACGTCTGCTATTGAGATTGCTCATAATCCTGTTCAGCATGACCGGACAAAACATGTGGAGGTAGATCGGCATTTCATTAAAGAAAACTTGGACAGGAAGATCATTCGTTTCCCTTTCGTGTATACGGAAGATCAATTAGCAGATGTTCTCACAAAAGGAGTGTCAAGAAAAGTGTTTGACAACTCAGTTGGCAAGTTGGGCATGATCGATATCTATGCCCCAACTTGA

Protein Analysis

699

Amino Acids

78.26

Weight (kDa)

6.91

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 213 - 457 5.9e-72 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1425
AccB1I GGYRCC 1 cut(s) 1425
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 4 cut(s) 411, 958, 1670, 1991
AciI CCGC 5 cut(s) 51, 90, 122, 429, 584
AclWI GGATC 4 cut(s) 112, 581, 594, 1095
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 4 cut(s) 199, 312, 1577, 1776
AfaI GTAC 4 cut(s) 1202, 1311, 1427, 1628
AfiI CCNNNNNNNGG 2 cut(s) 1210, 1583
AflII CTTAAG 2 cut(s) 44, 1565
AflIII ACRYGT 1 cut(s) 1921
AgsI TTSAA 9 cut(s) 497, 991, 1012, 1209, 1243, 1262, 1598, 1819, 1834
AhdI GACNNNNNGTC 1 cut(s) 1666
AjiI CACGTC 1 cut(s) 1872
AjnI CCWGG 4 cut(s) 277, 644, 943, 1576
AluBI AGCT 6 cut(s) 175, 302, 326, 1028, 1130, 1325
AluI AGCT 6 cut(s) 175, 302, 326, 1028, 1130, 1325
Alw26I GTCTC 7 cut(s) 215, 454, 568, 1023, 1322, 1352, 1438
AlwI GGATC 4 cut(s) 112, 581, 594, 1095
AlwNI CAGNNNCTG 2 cut(s) 284, 1637
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 4 cut(s) 52, 143, 391, 866
ApeKI GCWGC 1 cut(s) 158
ArsI GACNNNNNNTTYG 2 cut(s) 2015, 2047
Asp700I GAANNNNTTC 2 cut(s) 896, 1061
Asp718I GGTACC 1 cut(s) 1425
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 6 cut(s) 274, 345, 392, 425, 461, 1228
AsuHPI GGTGA 3 cut(s) 142, 1619, 1661
AsuII TTCGAA 1 cut(s) 832
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 5 cut(s) 274, 345, 425, 461, 1228
AxyI CCTNAGG 1 cut(s) 570
BaeI ACNNNNGTAYC 4 cut(s) 1184, 1217, 1417, 1450
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 1425
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 1064
BbvI GCAGC 1 cut(s) 145
BccI CCATC 4 cut(s) 135, 716, 1177, 1397
BceAI ACGGC 3 cut(s) 39, 254, 675
BcgI CGANNNNNNTGC 1 cut(s) 2066
BciT130I CCWGG 4 cut(s) 279, 646, 945, 1578
BciVI GTATCC 1 cut(s) 758
BcoDI GTCTC 7 cut(s) 215, 454, 568, 1023, 1322, 1352, 1438
BfaI CTAG 4 cut(s) 620, 1023, 1436, 1467
BfmI CTRYAG 3 cut(s) 306, 810, 1193
BfoI RGCGCY 1 cut(s) 390
BfrI CTTAAG 2 cut(s) 44, 1565
BfuI GTATCC 1 cut(s) 758
BglII AGATCT 1 cut(s) 194
BisI GCNGC 3 cut(s) 52, 91, 159
BlsI GCNGC 3 cut(s) 53, 92, 160
Bme1390I CCNGG 4 cut(s) 279, 646, 945, 1578
Bme18I GGWCC 5 cut(s) 274, 345, 425, 461, 1228
BmeRI GACNNNNNGTC 1 cut(s) 1666
BmeT110I CYCGRG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 1872
BmgT120I GGNCC 6 cut(s) 274, 345, 392, 425, 461, 1228
BmiI GGNNCC 6 cut(s) 276, 346, 427, 463, 588, 1427
BmrFI CCNGG 4 cut(s) 279, 646, 945, 1578
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 5 cut(s) 565, 677, 1528, 1624, 1785
BmuI ACTGGG 1 cut(s) 145
BoxI GACNNNNGTC 2 cut(s) 407, 423
BpiI GAAGAC 1 cut(s) 1064
Bpu14I TTCGAA 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 585, 1823
Bsa29I ATCGAT 1 cut(s) 2076
BsaBI GATNNNNATC 1 cut(s) 2078
BsaJI CCNNGG 7 cut(s) 270, 277, 382, 645, 943, 1231, 1848
BsaWI WCCGGW 1 cut(s) 1911
Bsc4I CCNNNNNNNGG 2 cut(s) 1210, 1583
Bse1I ACTGG 5 cut(s) 140, 295, 427, 673, 1336
Bse21I CCTNAGG 1 cut(s) 570
Bse3DI GCAATG 2 cut(s) 585, 1548
Bse8I GATNNNNATC 1 cut(s) 2078
BseBI CCWGG 4 cut(s) 279, 646, 945, 1578
BseCI ATCGAT 1 cut(s) 2076
BseDI CCNNGG 7 cut(s) 270, 277, 382, 645, 943, 1231, 1848
BseGI GGATG 6 cut(s) 146, 509, 580, 655, 692, 1543
BseJI GATNNNNATC 1 cut(s) 2078
BseLI CCNNNNNNNGG 2 cut(s) 1210, 1583
BseMI GCAATG 2 cut(s) 585, 1548
BseMII CTCAG 5 cut(s) 219, 359, 584, 1317, 2067
BseNI ACTGG 5 cut(s) 140, 295, 427, 673, 1336
BseRI GAGGAG 1 cut(s) 558
BseX3I CGGCCG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 2 cut(s) 1296, 1520
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 4 cut(s) 54, 145, 393, 868
BshNI GGYRCC 1 cut(s) 1425
BshVI ATCGAT 1 cut(s) 2076
BsiEI CGRYCG 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 1912
BslFI GGGAC 6 cut(s) 260, 358, 411, 447, 471, 1503
BslI CCNNNNNNNGG 2 cut(s) 1210, 1583
BsmAI GTCTC 7 cut(s) 215, 454, 568, 1023, 1322, 1352, 1438
BsmFI GGGAC 6 cut(s) 260, 358, 411, 447, 471, 1503
BsmI GAATGC 2 cut(s) 898, 1341
BsnI GGCC 4 cut(s) 54, 145, 393, 868
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 87
Bsp19I CCATGG 1 cut(s) 1848
BspACI CCGC 5 cut(s) 51, 90, 122, 429, 584
BspANI GGCC 4 cut(s) 54, 145, 393, 868
BspCNI CTCAG 5 cut(s) 220, 360, 583, 1318, 2066
BspDI ATCGAT 1 cut(s) 2076
BspLI GGNNCC 6 cut(s) 276, 346, 427, 463, 588, 1427
BspPI GGATC 4 cut(s) 112, 581, 594, 1095
BspQI GCTCTTC 1 cut(s) 1521
BspT104I TTCGAA 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 1425
BspTI CTTAAG 2 cut(s) 44, 1565
BsrBI CCGCTC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 585, 1548
BsrI ACTGG 5 cut(s) 140, 295, 427, 673, 1336
BssECI CCNNGG 7 cut(s) 270, 277, 382, 645, 943, 1231, 1848
BssNAI GTATAC 1 cut(s) 1992
BssT1I CCWWGG 3 cut(s) 382, 1231, 1848
Bst1107I GTATAC 1 cut(s) 1992
Bst2UI CCWGG 4 cut(s) 279, 646, 945, 1578
Bst4CI ACNGT 7 cut(s) 10, 537, 682, 706, 829, 879, 1668
Bst6I CTCTTC 3 cut(s) 218, 915, 1521
BstAFI CTTAAG 2 cut(s) 44, 1565
BstBI TTCGAA 1 cut(s) 832
BstC8I GCNNGC 3 cut(s) 41, 292, 391
BstDEI CTNAG 5 cut(s) 228, 368, 570, 1326, 2053
BstDSI CCRYGG 2 cut(s) 270, 1848
BstENI CCTNNNNNAGG 2 cut(s) 1208, 1581
BstF5I GGATG 6 cut(s) 146, 509, 580, 655, 692, 1543
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstMAI GTCTC 7 cut(s) 215, 454, 568, 1023, 1322, 1352, 1438
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 4 cut(s) 167, 369, 1643, 2067
BstNI CCWGG 4 cut(s) 279, 646, 945, 1578
BstNSI RCATGY 2 cut(s) 940, 1925
BstPAI GACNNNNGTC 2 cut(s) 407, 423
BstSCI CCNGG 4 cut(s) 277, 644, 943, 1576
BstSFI CTRYAG 3 cut(s) 306, 810, 1193
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 1064
BstX2I RGATCY 2 cut(s) 194, 586
BstXI CCANNNNNNTGG 2 cut(s) 389, 779
BstYI RGATCY 2 cut(s) 194, 586
BstZ17I GTATAC 1 cut(s) 1992
BstZI CGGCCG 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 2076
Bsu36I CCTNAGG 1 cut(s) 570
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 4 cut(s) 54, 145, 393, 868
BsuTUI ATCGAT 1 cut(s) 2076
BtgI CCRYGG 2 cut(s) 270, 1848
BtgZI GCGATG 2 cut(s) 620, 981
BtrI CACGTC 1 cut(s) 1872
BtsCI GGATG 6 cut(s) 146, 509, 580, 655, 692, 1543
BtsIMutI CAGTG 2 cut(s) 420, 825
Cac8I GCNNGC 3 cut(s) 41, 292, 391
CaiI CAGNNNCTG 2 cut(s) 284, 1637
CfoI GCGC 1 cut(s) 389
Cfr13I GGNCC 6 cut(s) 274, 345, 392, 425, 461, 1228
ClaI ATCGAT 1 cut(s) 2076
CseI GACGC 1 cut(s) 296
Csp6I GTAC 4 cut(s) 1201, 1310, 1426, 1627
CspCI CAANNNNNGTGG 2 cut(s) 1905, 1940
CviAII CATG 6 cut(s) 937, 1781, 1849, 1907, 1922, 2071
CviQI GTAC 4 cut(s) 1201, 1310, 1426, 1627
DdeI CTNAG 5 cut(s) 228, 368, 570, 1326, 2053
DriI GACNNNNNGTC 1 cut(s) 1666
EaeI YGGCCR 1 cut(s) 52
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 3 cut(s) 218, 915, 1521
Eam1105I GACNNNNNGTC 1 cut(s) 1666
EarI CTCTTC 3 cut(s) 218, 915, 1521
EciI GGCGGA 2 cut(s) 137, 599
EclXI CGGCCG 1 cut(s) 52
Eco130I CCWWGG 3 cut(s) 382, 1231, 1848
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 2080
Eco47I GGWCC 5 cut(s) 274, 345, 425, 461, 1228
Eco52I CGGCCG 1 cut(s) 52
Eco57I CTGAAG 4 cut(s) 199, 312, 1577, 1776
Eco81I CCTNAGG 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 98
EcoNI CCTNNNNNAGG 2 cut(s) 1208, 1581
EcoO109I RGGNCCY 1 cut(s) 1228
EcoRII CCWGG 4 cut(s) 277, 644, 943, 1576
EcoRV GATATC 1 cut(s) 2080
EcoT14I CCWWGG 3 cut(s) 382, 1231, 1848
EcoT22I ATGCAT 1 cut(s) 1515
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 382, 1231, 1848
FaeI CATG 6 cut(s) 940, 1784, 1852, 1910, 1925, 2074
FaqI GGGAC 6 cut(s) 260, 358, 411, 447, 471, 1503
FatI CATG 6 cut(s) 936, 1780, 1848, 1906, 1921, 2070
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 1533
FblI GTMKAC 4 cut(s) 411, 958, 1670, 1991
Fnu4HI GCNGC 3 cut(s) 52, 91, 159
FokI GGATG 6 cut(s) 153, 496, 587, 662, 699, 1550
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 3 cut(s) 52, 91, 159
FspBI CTAG 4 cut(s) 620, 1023, 1436, 1467
GlaI GCGC 1 cut(s) 388
GluI GCNGC 3 cut(s) 52, 91, 159
GsaI CCCAGC 2 cut(s) 1300, 1524
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 4 cut(s) 54, 145, 393, 868
HapII CCGG 1 cut(s) 1912
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 389
Hin1II CATG 6 cut(s) 940, 1784, 1852, 1910, 1925, 2074
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HincII GTYRAC 2 cut(s) 412, 540
HindII GTYRAC 2 cut(s) 412, 540
HinfI GANTC 7 cut(s) 548, 598, 834, 948, 1041, 1375, 1804
HpaI GTTAAC 1 cut(s) 540
HpaII CCGG 1 cut(s) 1912
HphI GGTGA 3 cut(s) 142, 1619, 1661
Hpy166II GTNNAC 9 cut(s) 133, 256, 412, 540, 597, 959, 1629, 1671, 1992
Hpy188III TCNNGA 5 cut(s) 572, 838, 1397, 1840, 2034
Hpy8I GTNNAC 9 cut(s) 133, 256, 412, 540, 597, 959, 1629, 1671, 1992
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 463, 491, 1219, 1416
HpyCH4III ACNGT 7 cut(s) 10, 537, 682, 706, 829, 879, 1668
HpyCH4IV ACGT 3 cut(s) 1108, 1432, 1871
HpyCH4V TGCA 9 cut(s) 222, 578, 631, 851, 1268, 1513, 1541, 1742, 1858
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 369, 1643, 2067
HpyF3I CTNAG 5 cut(s) 228, 368, 570, 1326, 2053
HpySE526I ACGT 3 cut(s) 1108, 1432, 1871
Hsp92II CATG 6 cut(s) 940, 1784, 1852, 1910, 1925, 2074
HspAI GCGC 1 cut(s) 387
KpnI GGTACC 1 cut(s) 1429
KspAI GTTAAC 1 cut(s) 540
LguI GCTCTTC 1 cut(s) 1521
LmnI GCTCC 1 cut(s) 726
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 5 cut(s) 565, 677, 1528, 1624, 1785
MaeI CTAG 4 cut(s) 620, 1023, 1436, 1467
MaeII ACGT 3 cut(s) 1108, 1432, 1871
MaeIII GTNAC 3 cut(s) 335, 1159, 1708
MbiI CCGCTC 1 cut(s) 51
MfeI CAATTG 2 cut(s) 1238, 1365
MflI RGATCY 2 cut(s) 194, 586
MhlI GDGCHC 1 cut(s) 87
MlyI GAGTC 2 cut(s) 592, 957
MmeI TCCRAC 4 cut(s) 664, 952, 1433, 1675
Mph1103I ATGCAT 1 cut(s) 1515
MroXI GAANNNNTTC 2 cut(s) 896, 1061
MseI TTAA 5 cut(s) 45, 539, 708, 1566, 1947
MslI CAYNNNNRTG 1 cut(s) 1536
MspCI CTTAAG 2 cut(s) 44, 1565
MspI CCGG 1 cut(s) 1912
MspR9I CCNGG 4 cut(s) 279, 646, 945, 1578
MunI CAATTG 2 cut(s) 1238, 1365
Mva1269I GAATGC 2 cut(s) 898, 1341
MvaI CCWGG 4 cut(s) 279, 646, 945, 1578
MwoI GCNNNNNNNGC 4 cut(s) 167, 369, 1643, 2067
NcoI CCATGG 1 cut(s) 1848
NdeI CATATG 1 cut(s) 1533
NlaIII CATG 6 cut(s) 940, 1784, 1852, 1910, 1925, 2074
NlaIV GGNNCC 6 cut(s) 276, 346, 427, 463, 588, 1427
NmeAIII GCCGAG 4 cut(s) 66, 342, 347, 398
NmuCI GTSAC 2 cut(s) 335, 1708
NsiI ATGCAT 1 cut(s) 1515
NspI RCATGY 2 cut(s) 940, 1925
NspV TTCGAA 1 cut(s) 832
PaeR7I CTCGAG 1 cut(s) 98
PciI ACATGT 1 cut(s) 1921
PciSI GCTCTTC 1 cut(s) 1521
PctI GAATGC 2 cut(s) 898, 1341
PdmI GAANNNNTTC 2 cut(s) 896, 1061
PfeI GAWTC 5 cut(s) 548, 834, 1041, 1375, 1804
PkrI GCNGC 3 cut(s) 53, 92, 160
PleI GAGTC 2 cut(s) 592, 956
PpsI GAGTC 2 cut(s) 592, 956
PpuMI RGGWCCY 1 cut(s) 1228
PscI ACATGT 1 cut(s) 1921
PshAI GACNNNNGTC 2 cut(s) 407, 423
Psp5II RGGWCCY 1 cut(s) 1228
Psp6I CCWGG 4 cut(s) 277, 644, 943, 1576
PspFI CCCAGC 2 cut(s) 1296, 1520
PspGI CCWGG 4 cut(s) 277, 644, 943, 1576
PspN4I GGNNCC 6 cut(s) 276, 346, 427, 463, 588, 1427
PspPI GGNCC 6 cut(s) 274, 345, 392, 425, 461, 1228
PspPPI RGGWCCY 1 cut(s) 1228
PspXI VCTCGAGB 1 cut(s) 98
PstNI CAGNNNCTG 2 cut(s) 284, 1637
PsuI RGATCY 2 cut(s) 194, 586
RsaI GTAC 4 cut(s) 1202, 1311, 1427, 1628
RsaNI GTAC 4 cut(s) 1201, 1310, 1426, 1627
RseI CAYNNNNRTG 1 cut(s) 1536
SalI GTCGAC 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 1521
SaqAI TTAA 5 cut(s) 45, 539, 708, 1566, 1947
SatI GCNGC 3 cut(s) 52, 91, 159
Sau96I GGNCC 6 cut(s) 274, 345, 392, 425, 461, 1228
SchI GAGTC 2 cut(s) 592, 957
ScrFI CCNGG 4 cut(s) 279, 646, 945, 1578
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 5 cut(s) 565, 677, 1528, 1624, 1785
SfcI CTRYAG 3 cut(s) 306, 810, 1193
Sfr274I CTCGAG 1 cut(s) 98
SfuI TTCGAA 1 cut(s) 832
SinI GGWCC 5 cut(s) 274, 345, 425, 461, 1228
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 1536
SmlI CTYRAG 5 cut(s) 44, 98, 600, 1565, 1838
SmoI CTYRAG 5 cut(s) 44, 98, 600, 1565, 1838
SsiI CCGC 5 cut(s) 51, 90, 122, 429, 584
SspMI CTAG 4 cut(s) 620, 1023, 1436, 1467
StyD4I CCNGG 4 cut(s) 277, 644, 943, 1576
StyI CCWWGG 3 cut(s) 382, 1231, 1848
TaaI ACNGT 7 cut(s) 10, 537, 682, 706, 829, 879, 1668
TaiI ACGT 3 cut(s) 1111, 1435, 1874
TaqI TCGA 7 cut(s) 60, 99, 411, 641, 832, 1768, 2076
TatI WGTACW 1 cut(s) 1309
TauI GCSGC 2 cut(s) 54, 93
TfiI GAWTC 5 cut(s) 548, 834, 1041, 1375, 1804
Tru1I TTAA 5 cut(s) 45, 539, 708, 1566, 1947
Tru9I TTAA 5 cut(s) 45, 539, 708, 1566, 1947
TscAI CASTG 2 cut(s) 427, 832
TseFI GTSAC 2 cut(s) 335, 1708
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 2 cut(s) 335, 1708
TspDTI ATGAA 9 cut(s) 623, 627, 647, 807, 1070, 1090, 1238, 1388, 1933
TspGWI ACGGA 1 cut(s) 2009
TspRI CASTG 2 cut(s) 427, 832
Vha464I CTTAAG 2 cut(s) 44, 1565
VpaK11BI GGWCC 5 cut(s) 274, 345, 425, 461, 1228
XagI CCTNNNNNAGG 2 cut(s) 1208, 1581
XceI RCATGY 2 cut(s) 940, 1925
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 4 cut(s) 411, 958, 1670, 1991
XmnI GAANNNNTTC 2 cut(s) 896, 1061
XspI CTAG 4 cut(s) 620, 1023, 1436, 1467
Zsp2I ATGCAT 1 cut(s) 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.