RchiOBHm_Chr2g0148361

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Forward (+)
66048413 .. 66050290
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51794

Sequence Viewer

Length: 1533 bp
ATGTTTCTATTCCATCTTCTAATACTCTTCCTGCTCTTGTCTTTGGTTGTATTGCTTATGTTCATCTATATAAGAATCAGAGGTCAAAGTTGGATGTTAGAGCCCTTAAGTATGTGTTTGTGGGGTATGGTTCTCATCAGAAAGGTTACAAATGTTATCATCCTCAATCCCAGAATTTTTATGTCACCATGGATGTTACGTTCAGTGAAGATGCATGTTATTTTTTTGCCTCCTGTGACTCCTCGTCAGGGGGAGAAGTCTTGTTATTATGAAGATTTATTTAGTGGGCAAGATGGGAGACTAGCATCCGAGTTCTTAAGGCTGGAGTGTTTTGGAACGGAACTTGAAAAAGAGGACAGCAGCCCACCGAATGGAGAAGAGAATTTGGGTAGTCAGCTACTGACCAGTCTACCGGATCAGTCTGACCGATCGGTTGAAGAGGAAGTTTCTGATTCTGTTTTGGATGAAATTCTTGATGTCACCAATGCCGTTTTGAACAGTTCTTCTGTCTCTCCCTCTCCTTTAGTGGTCTCTCCTGCTCAATCATCACCCGAGGATGCAATGAGGGATGAGAAGTGGATGCAAGCAATGACAGTCAAAATGGATGCTCTTGAGAAGAATTGTACGTGGGAGTTAGTATCATTACCACCCGGGAAGAAGACAGTTGGTTGTCGGTGGGTGTATATTGTGAAACACAATTCAGATGGATCGGTGGACAGGTACAAGGCAAGACTAGTGGCTAAAGGCTATACTCAGAAGTATGGAGTGGATTATGATGAGACATTTGCACCAGTGGCCAAAATTAACACAATTCGGGTTTATATGGATTTGCCTCCTGGATATGGTACTTCTACTGGAGAGCAGGTGGTGTGCAGATTGAAAAAATCTCTTTATGGTTTGAAGCAGTCTCCCAGAGCTTGGTTTGGCAGGTTCACCAAGTTCATGAAGAAAATTAGCTACCGGCAAAGCAATTCAGATCACACCTTGTTCCTTAAACATCGGTGTGGTAAAGTGACTGCCTTGATTATTTATGTTGATGACATGGTTGTGACAGGTGATGACTTTGAGGAGATTCAGAGGTTACAAGGTCAGTTATCTTTAGAATTTGAGATGAAAGATTTGGGTAATTTGAAATATTTCTTGGGAATTGAAGTTGCTCGTGGGAATGATTGTATTGTGTTGAGTCAGAGGAAGTATGTTCTCGACTTGCTGGCAGAAACATGTATGCTTGACTGTCAACCTGTTGATACTCCTATTGAGCAAAATCATCGGTTAGCAGAGTACTTGGATCAAGTACCTACAAATAAAGCAAGATACCAGAGGTTAGTTGGCCGGTTGATTTATTTGTCCCACACTAGACCAGATTTAGCCTATGCTGTTAGTGTGGTGAGTCAGTTTATGCATAATCCCAGTAAGACTCATATGGATGCTGTATTTCGTATTTTGCTGTATTTAAAGTTTGCTCCAGGGAAGGGATTGCTCTTTTCAAAACACATTCACTTGGATGTTTCCAGGTACACAAACGCGGACTAG

Protein Analysis

510

Amino Acids

58.28

Weight (kDa)

7.14

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 208 - 273 2.9e-18 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 272 - 420 9.2e-35 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 853
Acc36I ACCTGC 2 cut(s) 853, 918
AccB7I CCANNNNNTGG 2 cut(s) 371, 918
AccI GTMKAC 1 cut(s) 409
AccII CGCG 1 cut(s) 1526
AciI CCGC 1 cut(s) 1526
AclWI GGATC 3 cut(s) 423, 715, 1296
AcoI YGGCCR 2 cut(s) 795, 1330
AcsI RAATTY 4 cut(s) 174, 382, 468, 1103
AfaI GTAC 6 cut(s) 625, 722, 847, 1283, 1296, 1517
AfiI CCNNNNNNNGG 5 cut(s) 248, 371, 842, 918, 1472
AflII CTTAAG 2 cut(s) 106, 316
AflIII ACRYGT 1 cut(s) 1220
AgsI TTSAA 8 cut(s) 347, 437, 496, 880, 901, 1132, 1151, 1488
AhdI GACNNNNNGTC 1 cut(s) 243
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 3 cut(s) 835, 1465, 1511
AjuI GAANNNNNNNTTGG 2 cut(s) 1124, 1156
AloI GAACNNNNNNTCC 2 cut(s) 184, 216
AluBI AGCT 3 cut(s) 397, 917, 957
AluI AGCT 3 cut(s) 397, 917, 957
Alw26I GTCTC 5 cut(s) 292, 514, 535, 773, 912
AlwI GGATC 3 cut(s) 423, 715, 1296
AlwNI CAGNNNCTG 1 cut(s) 400
Ama87I CYCGRG 2 cut(s) 551, 650
AoxI GGCC 2 cut(s) 795, 1330
ApeKI GCWGC 1 cut(s) 360
ApoI RAATTY 4 cut(s) 174, 382, 468, 1103
Asp700I GAANNNNTTC 1 cut(s) 1136
AsuC2I CCSGG 2 cut(s) 651, 652
AsuHPI GGTGA 6 cut(s) 177, 472, 540, 925, 1067, 1399
AvaI CYCGRG 2 cut(s) 551, 650
BalI TGGCCA 1 cut(s) 797
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 1158
BbsI GAAGAC 1 cut(s) 665
BbvI GCAGC 1 cut(s) 372
BccI CCATC 3 cut(s) 21, 287, 698
BceAI ACGGC 1 cut(s) 473
BcgI CGANNNNNNTGC 2 cut(s) 1250, 1284
BciT130I CCWGG 3 cut(s) 837, 1467, 1513
BcnI CCSGG 2 cut(s) 651, 652
BcoDI GTCTC 5 cut(s) 292, 514, 535, 773, 912
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 4 cut(s) 302, 734, 1356, 1531
BfrI CTTAAG 2 cut(s) 106, 316
BfuAI ACCTGC 2 cut(s) 853, 918
BisI GCNGC 1 cut(s) 361
BlsI GCNGC 1 cut(s) 362
BmcAI AGTACT 1 cut(s) 1283
Bme1390I CCNGG 5 cut(s) 651, 652, 837, 1467, 1513
BmeRI GACNNNNNGTC 1 cut(s) 243
BmeT110I CYCGRG 2 cut(s) 551, 650
BmrFI CCNGG 5 cut(s) 651, 652, 837, 1467, 1513
BmrI ACTGGG 1 cut(s) 1404
BmsI GCATC 6 cut(s) 201, 314, 547, 570, 595, 1417
BmuI ACTGGG 1 cut(s) 1404
BpiI GAAGAC 1 cut(s) 665
BpmI CTGGAG 3 cut(s) 344, 876, 1449
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 2 cut(s) 651, 652
BsaAI YACGTR 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 4 cut(s) 188, 552, 650, 1466
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 371, 842, 918, 1472
Bse118I RCCGGY 2 cut(s) 960, 1332
Bse1I ACTGG 4 cut(s) 405, 791, 859, 1410
Bse3DI GCAATG 2 cut(s) 567, 594
BseBI CCWGG 3 cut(s) 837, 1467, 1513
BseDI CCNNGG 4 cut(s) 188, 552, 650, 1466
BseLI CCNNNNNNNGG 5 cut(s) 248, 371, 842, 918, 1472
BseMI GCAATG 2 cut(s) 567, 594
BseMII CTCAG 1 cut(s) 767
BseNI ACTGG 4 cut(s) 405, 791, 859, 1410
BseRI GAGGAG 2 cut(s) 231, 1082
BseXI GCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 892
Bsh1236I CGCG 1 cut(s) 1526
Bsh1285I CGRYCG 1 cut(s) 431
BshFI GGCC 2 cut(s) 797, 1332
BsiEI CGRYCG 1 cut(s) 431
BsiHKCI CYCGRG 2 cut(s) 551, 650
BsiSI CCGG 4 cut(s) 413, 651, 961, 1333
BslFI GGGAC 1 cut(s) 1333
BslI CCNNNNNNNGG 5 cut(s) 248, 371, 842, 918, 1472
BsmAI GTCTC 5 cut(s) 292, 514, 535, 773, 912
BsmFI GGGAC 1 cut(s) 1333
BsnI GGCC 2 cut(s) 797, 1332
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 2 cut(s) 551, 650
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 5 cut(s) 415, 428, 707, 976, 1288
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 1 cut(s) 1526
BspANI GGCC 2 cut(s) 797, 1332
BspCNI CTCAG 1 cut(s) 766
BspFNI CGCG 1 cut(s) 1526
BspHI TCATGA 1 cut(s) 942
BspMI ACCTGC 2 cut(s) 853, 918
BspPI GGATC 3 cut(s) 423, 715, 1296
BspTI CTTAAG 2 cut(s) 106, 316
BspTNI GGTCTC 1 cut(s) 535
BsrDI GCAATG 2 cut(s) 567, 594
BsrFI RCCGGY 2 cut(s) 960, 1332
BsrI ACTGG 4 cut(s) 405, 791, 859, 1410
BssAI RCCGGY 2 cut(s) 960, 1332
BssECI CCNNGG 4 cut(s) 188, 552, 650, 1466
BssMI GATC 5 cut(s) 415, 428, 707, 976, 1288
BssSI CACGAG 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 1158
Bst2UI CCWGG 3 cut(s) 837, 1467, 1513
Bst4CI ACNGT 4 cut(s) 500, 595, 664, 1235
Bst6I CTCTTC 3 cut(s) 32, 372, 432
BstAFI CTTAAG 2 cut(s) 106, 316
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 585, 1212
BstDEI CTNAG 1 cut(s) 753
BstDSI CCRYGG 1 cut(s) 188
BstFNI CGCG 1 cut(s) 1526
BstKTI GATC 5 cut(s) 418, 431, 710, 979, 1291
BstMAI GTCTC 5 cut(s) 292, 514, 535, 773, 912
BstMBI GATC 5 cut(s) 415, 428, 707, 976, 1288
BstMCI CGRYCG 1 cut(s) 431
BstMWI GCNNNNNNNGC 1 cut(s) 794
BstNI CCWGG 3 cut(s) 837, 1467, 1513
BstNSI RCATGY 2 cut(s) 218, 1224
BstSCI CCNGG 5 cut(s) 649, 650, 835, 1465, 1511
BstUI CGCG 1 cut(s) 1526
BstV1I GCAGC 1 cut(s) 372
BstV2I GAAGAC 1 cut(s) 665
BsuRI GGCC 2 cut(s) 797, 1332
BtgI CCRYGG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 210, 798
BveI ACCTGC 2 cut(s) 853, 918
Cac8I GCNNGC 2 cut(s) 585, 1212
CaiI CAGNNNCTG 1 cut(s) 400
CciI TCATGA 1 cut(s) 942
Cfr10I RCCGGY 2 cut(s) 960, 1332
Cfr9I CCCGGG 1 cut(s) 650
Csp6I GTAC 6 cut(s) 624, 721, 846, 1282, 1295, 1516
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 5 cut(s) 189, 215, 943, 1042, 1221
CviQI GTAC 6 cut(s) 624, 721, 846, 1282, 1295, 1516
DdeI CTNAG 1 cut(s) 753
DpnI GATC 5 cut(s) 417, 430, 709, 978, 1290
DpnII GATC 5 cut(s) 415, 428, 707, 976, 1288
DraI TTTAAA 1 cut(s) 1455
DriI GACNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 795, 1330
Eam1104I CTCTTC 3 cut(s) 32, 372, 432
Eam1105I GACNNNNNGTC 1 cut(s) 243
EarI CTCTTC 3 cut(s) 32, 372, 432
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 535
Eco88I CYCGRG 2 cut(s) 551, 650
EcoRII CCWGG 3 cut(s) 835, 1465, 1511
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 2 cut(s) 216, 1404
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 5 cut(s) 192, 218, 946, 1045, 1224
FaqI GGGAC 1 cut(s) 1333
FatI CATG 5 cut(s) 188, 214, 942, 1041, 1220
FauNDI CATATG 1 cut(s) 1422
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 361
FspBI CTAG 4 cut(s) 302, 734, 1356, 1531
GluI GCNGC 1 cut(s) 361
GsuI CTGGAG 3 cut(s) 344, 876, 1449
HaeIII GGCC 2 cut(s) 797, 1332
HapII CCGG 4 cut(s) 413, 651, 961, 1333
Hin1II CATG 5 cut(s) 192, 218, 946, 1045, 1224
HincII GTYRAC 1 cut(s) 1238
HindII GTYRAC 1 cut(s) 1238
HinfI GANTC 7 cut(s) 75, 238, 452, 1072, 1183, 1390, 1417
HpaII CCGG 4 cut(s) 413, 651, 961, 1333
HphI GGTGA 6 cut(s) 177, 472, 540, 925, 1067, 1399
Hpy166II GTNNAC 5 cut(s) 410, 715, 933, 1238, 1518
Hpy188III TCNNGA 4 cut(s) 473, 611, 943, 1202
Hpy8I GTNNAC 5 cut(s) 410, 715, 933, 1238, 1518
HpyAV CCTTC 1 cut(s) 1465
HpyCH4III ACNGT 4 cut(s) 500, 595, 664, 1235
HpyCH4IV ACGT 2 cut(s) 199, 626
HpyCH4V TGCA 6 cut(s) 214, 560, 583, 788, 873, 1402
HpyF10VI GCNNNNNNNGC 1 cut(s) 794
HpyF3I CTNAG 1 cut(s) 753
HpySE526I ACGT 2 cut(s) 199, 626
Hsp92II CATG 5 cut(s) 192, 218, 946, 1045, 1224
Kzo9I GATC 5 cut(s) 415, 428, 707, 976, 1288
LmnI GCTCC 1 cut(s) 1468
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 6 cut(s) 201, 314, 547, 570, 595, 1417
MaeI CTAG 4 cut(s) 302, 734, 1356, 1531
MaeII ACGT 2 cut(s) 199, 626
MaeIII GTNAC 8 cut(s) 145, 183, 195, 235, 478, 1012, 1048, 1080
MalI GATC 5 cut(s) 417, 430, 709, 978, 1290
MboI GATC 5 cut(s) 415, 428, 707, 976, 1288
MhlI GDGCHC 1 cut(s) 105
MlsI TGGCCA 1 cut(s) 797
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 4 cut(s) 232, 1192, 1399, 1411
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 797
Mph1103I ATGCAT 2 cut(s) 216, 1404
MroXI GAANNNNTTC 1 cut(s) 1136
MscI TGGCCA 1 cut(s) 797
MseI TTAA 5 cut(s) 107, 317, 804, 993, 1454
MslI CAYNNNNRTG 4 cut(s) 1002, 1046, 1425, 1503
Msp20I TGGCCA 1 cut(s) 797
MspCI CTTAAG 2 cut(s) 106, 316
MspI CCGG 4 cut(s) 413, 651, 961, 1333
MspR9I CCNGG 5 cut(s) 651, 652, 837, 1467, 1513
MvaI CCWGG 3 cut(s) 837, 1467, 1513
MvnI CGCG 1 cut(s) 1526
MwoI GCNNNNNNNGC 1 cut(s) 794
NciI CCSGG 2 cut(s) 651, 652
NcoI CCATGG 1 cut(s) 188
NdeI CATATG 1 cut(s) 1422
NdeII GATC 5 cut(s) 415, 428, 707, 976, 1288
NlaIII CATG 5 cut(s) 192, 218, 946, 1045, 1224
NmuCI GTSAC 5 cut(s) 183, 235, 478, 1012, 1048
NsiI ATGCAT 2 cut(s) 216, 1404
NspI RCATGY 2 cut(s) 218, 1224
PagI TCATGA 1 cut(s) 942
PaqCI CACCTGC 1 cut(s) 853
PciI ACATGT 1 cut(s) 1220
PdmI GAANNNNTTC 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 75, 452, 1072
PflMI CCANNNNNTGG 2 cut(s) 371, 918
PfoI TCCNGGA 1 cut(s) 835
PkrI GCNGC 1 cut(s) 362
Ple19I CGATCG 1 cut(s) 431
PleI GAGTC 4 cut(s) 232, 1191, 1398, 1411
PpsI GAGTC 4 cut(s) 232, 1191, 1398, 1411
Ppu21I YACGTR 1 cut(s) 627
PscI ACATGT 1 cut(s) 1220
Psp6I CCWGG 3 cut(s) 835, 1465, 1511
PspGI CCWGG 3 cut(s) 835, 1465, 1511
PstNI CAGNNNCTG 1 cut(s) 400
PvuI CGATCG 1 cut(s) 431
RsaI GTAC 6 cut(s) 625, 722, 847, 1283, 1296, 1517
RsaNI GTAC 6 cut(s) 624, 721, 846, 1282, 1295, 1516
RseI CAYNNNNRTG 4 cut(s) 1002, 1046, 1425, 1503
SaqAI TTAA 5 cut(s) 107, 317, 804, 993, 1454
SatI GCNGC 1 cut(s) 361
Sau3AI GATC 5 cut(s) 415, 428, 707, 976, 1288
ScaI AGTACT 1 cut(s) 1283
SchI GAGTC 4 cut(s) 232, 1192, 1399, 1411
ScrFI CCNGG 5 cut(s) 651, 652, 837, 1467, 1513
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 6 cut(s) 201, 314, 547, 570, 595, 1417
SmaI CCCGGG 1 cut(s) 652
SmiMI CAYNNNNRTG 4 cut(s) 1002, 1046, 1425, 1503
SmlI CTYRAG 3 cut(s) 106, 316, 611
SmoI CTYRAG 3 cut(s) 106, 316, 611
SpeI ACTAGT 1 cut(s) 733
SsiI CCGC 1 cut(s) 1526
SspI AATATT 1 cut(s) 1136
SspMI CTAG 4 cut(s) 302, 734, 1356, 1531
StyD4I CCNGG 5 cut(s) 649, 650, 835, 1465, 1511
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 4 cut(s) 500, 595, 664, 1235
TaiI ACGT 2 cut(s) 202, 629
TaqI TCGA 1 cut(s) 1203
TaqII GACCGA 1 cut(s) 441
TatI WGTACW 1 cut(s) 1281
TfiI GAWTC 3 cut(s) 75, 452, 1072
Tru1I TTAA 5 cut(s) 107, 317, 804, 993, 1454
Tru9I TTAA 5 cut(s) 107, 317, 804, 993, 1454
TscAI CASTG 2 cut(s) 210, 798
TseFI GTSAC 5 cut(s) 183, 235, 478, 1012, 1048
TseI GCWGC 1 cut(s) 360
Tsp45I GTSAC 5 cut(s) 183, 235, 478, 1012, 1048
TspDTI ATGAA 6 cut(s) 52, 285, 480, 931, 959, 1127
TspGWI ACGGA 1 cut(s) 353
TspMI CCCGGG 1 cut(s) 650
TspRI CASTG 2 cut(s) 210, 798
Van91I CCANNNNNTGG 2 cut(s) 371, 918
Vha464I CTTAAG 2 cut(s) 106, 316
XapI RAATTY 4 cut(s) 174, 382, 468, 1103
XceI RCATGY 2 cut(s) 218, 1224
XcmI CCANNNNNNNNNTGG 1 cut(s) 1325
XmaI CCCGGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 1136
XspI CTAG 4 cut(s) 302, 734, 1356, 1531
ZrmI AGTACT 1 cut(s) 1283
Zsp2I ATGCAT 2 cut(s) 216, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.