Rroxscaffold_3G00240550

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000071
Physical Location & Seq
Forward (+)
32340155 .. 32342356
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240550.1

Sequence Viewer

Length: 351 bp
ATGATTGTCACAGGGGATGATCTTAAAGAGATGGAGGCTCTACAAAAATATCTTTCTAAGGAATTCGAGATGAAGGACCTTGGGCAATTGAAGTATTTTCTTGGAATTGAAGTTGCAAGGTCTAAGAAGGGGATTTCTTTGTCCCAACGAAAGTATGTACTTGACTTGTTAGGCGAGACCGAATGCTTGACTCGCAACCCTATTGAGACACGGATCGAGATGAACCACAACCTCGCCATCTATCCGGATCGTGTTCCAACTAATAAAGGAAGGTACCAACATCTAGTAGGAAGGCTTATTTATCTTTCTCATACTAGGCCCGATATTGCTTATGCTTGTGAGTGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

116

Amino Acids

13.39

Weight (kDa)

7.71

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 71 2.9e-12 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 273
AccIII TCCGGA 1 cut(s) 244
AclWI GGATC 2 cut(s) 221, 255
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 2 cut(s) 159, 275
AgsI TTSAA 2 cut(s) 91, 110
Alw26I GTCTC 2 cut(s) 170, 200
AlwI GGATC 2 cut(s) 221, 255
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 62
Asp718I GGTACC 1 cut(s) 273
AspS9I GGNCC 2 cut(s) 76, 318
AvaII GGWCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 273
BccI CCATC 2 cut(s) 25, 245
BcoDI GTCTC 2 cut(s) 170, 200
BfaI CTAG 2 cut(s) 284, 315
Bme18I GGWCC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 76, 318
BmiI GGNNCC 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 79
BsaWI WCCGGW 1 cut(s) 244
BseAI TCCGGA 1 cut(s) 244
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 1 cut(s) 22
BshFI GGCC 1 cut(s) 319
BshNI GGYRCC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 127
BsmAI GTCTC 2 cut(s) 170, 200
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 1 cut(s) 319
Bso31I GGTCTC 1 cut(s) 170
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 3 cut(s) 19, 213, 247
BspANI GGCC 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 275
BspPI GGATC 2 cut(s) 221, 255
BspT107I GGYRCC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 3 cut(s) 19, 213, 247
BssT1I CCWWGG 1 cut(s) 79
BstDEI CTNAG 2 cut(s) 57, 123
BstF5I GGATG 1 cut(s) 22
BstKTI GATC 3 cut(s) 22, 216, 250
BstMAI GTCTC 2 cut(s) 170, 200
BstMBI GATC 3 cut(s) 19, 213, 247
BstMWI GCNNNNNNNGC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 76, 318
Csp6I GTAC 2 cut(s) 158, 274
CviJI RGCY 3 cut(s) 38, 295, 319
CviKI_1 RGCY 3 cut(s) 38, 295, 319
CviQI GTAC 2 cut(s) 158, 274
DdeI CTNAG 2 cut(s) 57, 123
DpnI GATC 3 cut(s) 21, 215, 249
DpnII GATC 3 cut(s) 19, 213, 247
Eco130I CCWWGG 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 79
FaiI YATR 3 cut(s) 156, 312, 333
FaqI GGGAC 1 cut(s) 127
FokI GGATG 1 cut(s) 29
FspBI CTAG 2 cut(s) 284, 315
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 245
HinfI GANTC 1 cut(s) 190
HpaII CCGG 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 67, 217, 245
HpyAV CCTTC 4 cut(s) 67, 121, 264, 285
HpyCH4V TGCA 1 cut(s) 116
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 2 cut(s) 57, 123
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 19, 213, 247
LpnPI CCDG 1 cut(s) 258
MaeI CTAG 2 cut(s) 284, 315
MaeIII GTNAC 1 cut(s) 7
MalI GATC 3 cut(s) 21, 215, 249
MboI GATC 3 cut(s) 19, 213, 247
MfeI CAATTG 1 cut(s) 86
MluCI AATT 3 cut(s) 62, 86, 105
MlyI GAGTC 1 cut(s) 184
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 2 cut(s) 28, 242
MroI TCCGGA 1 cut(s) 244
MseI TTAA 1 cut(s) 24
MspI CCGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 192
NdeII GATC 3 cut(s) 19, 213, 247
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 7
PctI GAATGC 1 cut(s) 188
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 2 cut(s) 76, 318
PspPPI RGGWCCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 159, 275
RsaNI GTAC 2 cut(s) 158, 274
SaqAI TTAA 1 cut(s) 24
Sau3AI GATC 3 cut(s) 19, 213, 247
Sau96I GGNCC 2 cut(s) 76, 318
SchI GAGTC 1 cut(s) 184
SetI ASST 4 cut(s) 81, 122, 234, 275
SinI GGWCC 1 cut(s) 76
Sse9I AATT 3 cut(s) 62, 86, 105
SspMI CTAG 2 cut(s) 284, 315
StyI CCWWGG 1 cut(s) 79
TaqI TCGA 2 cut(s) 66, 216
TaqII GACCGA 1 cut(s) 194
TasI AATT 3 cut(s) 62, 86, 105
TatI WGTACW 1 cut(s) 157
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseFI GTSAC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 86, 236
TspGWI ACGGA 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 62
XspI CTAG 2 cut(s) 284, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.