RchiOBHm_Chr3g0463471

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000071
Physical Location & Seq
Forward (+)
10814312 .. 10817816
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42978

Sequence Viewer

Length: 3156 bp
ATGGCTACTCGATACAGACCGCCGCATGTTCGTAACTTCCAGCCTTCTTCATCTACATCACCCAGGAAAGCACCTTTTTCAGTTCCCATGAATCACTCTGGTAACTCCACTTTTACCTTCATGACCACTTCAGGTAAACCTTACTCTCTGCTATCGAGTAACTCTGACTTTAATTGGAAGGATATGTGGATAATAGATTCAGGTGCAACGGACCATATGACTAATAACTCTAGCAATATTCACCATTTAACTTCAGTTCACCGGTCTGGCGTAACTAATGCCAATGGCGATTCCTATCCAATTACAGGTGCTGGGTCTGTCACTCTTACAGACTCCATTACCCTTGATACAGTGTTACTTGTTCCTTCCCTATCTCATAATCTCTTGTCTGTCAAACAGATAACAAAACAATTTTATTGTTGTGTAATTCTTTACCCGTGGTGTTGTATATTTCAGGACATCCTAACAGGGGAAATCATTGGGCGGGGTATTGAGAAGGGGGGGTTATACTACATGGAGATGACCACTGCTGGCAATCATCGTGTAAGTGAAGTGCATCAGATTCAAAAAGGAACTTCCACAAATGAAGAACAGATATGGTTATGGCATAAGAGATTGGGACATCCATCATTCGGTTATCTTCAAACTCTCTTCCCTTTATTGTTTCAACATAATAAACCATCCGCCTTTAAATGCGACACTTGCATTTTAGGCAAGAGTCACCGTGTCAGCTATCCATTGAGCTCCACTAAAAGTATTGCCCCTTTTGATCTTGTTCACTCTGATGTCTGGGGACCTTGTCCACCAACCCCCTCTGGAATGAAATGGTTTGTTTTATTTGTCGATGATTGCACCCGGATGAGTTGGATCTACTTGCTGAAAAATAAAAGTGATGTTTTCCCAGTTTTTCAGACTTTTCATGCCATGATCCGAACTCAGTTCCAAACACCTATTAAAGTCCTTCGTTCAGATAATGGTGGAGAATATGTGAACCATCTCTTTCACCAGTATCTAGCTACCCATGGCATCATTCATCAGACCACATGTCCTCAAACTCCTCAACAAAATGGTGTGGCGGAAAGAAAGAACCGCCACATTCTTGAAGTTGCTCGCTCCATGTTGATCGGAGCTCATATGCCAGAATCTTTTTGGGGGGATGCCATCCTAACTACTGTTTACCTCATAAATAGACTTCCTACTCAAGCCCTTCAGTCTACATCTAAGGAAACCAGTCCACCACCACAAACACCAATTCAAGCTCTTGCCAGTCATTTACCTTTACCACCTACCCATGCCCTTGATCCAAAGATTTTTGGTTGTGTAGCTTATGTACATATCCATCGAAACCAGAGGACTAAGTTGGATGCTTGTGCTGAAAAGTGTATCTTTATTGGCTATGCTTCCACTCAAAAGGGATATCGGTGTTATAGTCCAACCACTAAACGTACCTATGTTTCCATGGATGTTACATTCCATGAACATGATCTACATTTTTCCCCGAAGGCTACTCTTCAGGGGGAGAATGATATTGAGGAAGAGCAGTCTTCGAGGGATTGCTTGTGTCCACCCTTGGAACCCATGGACATCCAAGAGCATGGAAGTACTGGAGAAACAAGAATTGAGCATGGAAATACTGAAAGGAGCAACCAGTTACCGACATCAGAAGCAAACTCTAGAATCGTTGAAAATGGAAATCAGCTGCCAACTCCAGAATCACCAGTAAGAGAGATTTCTTCCCCTTCTACTATCCCATCAACACCAATGCTCCCTGAGGATGATCCAGAGGTAATTAATAACCCTGTGTTTGAATTTCATGATAATATAAATTCTGAGCCTAACACTGAAACGGAACCAAGTCATAAATTGCCATTTCGAAAAAATAGAGGTCAACCAAAGGCTCAATATGAACCAGATCCTAAAGCCAAGGCCAAATATCCCATAAGCAATTACATGTCATCCCATAGGTTATCCAAGTCATATGCGTCCTTTGTATTTCATTTATCTGCTATTATCATTCCAAATAAAGTACAGGATGCTCTAGCAGATCGTAAGTGGACTAAAGCTATGGAAGAGGAAATGGAAGCCTTGCAAAAGAGTCAAACATGGGAACTAGTGCCAAAACCAGCAGGGAAAAGAATAGTAGGGTGCAGATGGATCTATACTTTGAAGTACGCAGCTGATGGTACATTGGATAGATATAAAGCCAGGTTGGTTGCTAAAGGGTATACTCAGACATATGGTATTGACTATCAAGAGACTTTTGCACCGGTGGCCAAGATGAATACTGTTCGAGTACTACTGTCATTATTAGCGGCTAACCTTGACTGGCCTCTGAAGCAATTTGATGTAAAAAATGCCTTCCTACATGGAGACTTGAAGGAAGAAGTCTACATGGATCTACCTCCTGGCTATTCTCTACCTTCACAAGCAGGAATGGTGTGCAAGTTGAAAAAATCCCTTTATGGACTAAAACAGTCCCCCAGGGCATGGTTTGGGAGATTCAGCCAATCTATGAAACAGTTTGGTTATAAACAAAGCAACTCTGACCATACACTGTTTCTGAAACACAGAAAAGATAAACTTACTGCACTTATCATTTATGTTGATGATATGATTGTGACAGGTAATGACCTAGAAGAAGTCAAGAAACTAGAAGCACATCTATCATCTGTTTTTGAGATGAAAGATCTTGGAGGATTGAAATACTTCTTGGGCATTGAGGTGGCTAGATCAAGAGAAGGCATTTCCCTATCTCAAAGGAAATATGTGGTTGACTTACTCATAGAGACTGGTTTACTAGCATGTCAACCAGCAGATACACCGATGGAGCAAAATCACAAATTAGCAGAATATCCAGACCAAGTGCCTACTAACAAAGAGAGATATCAAAGGTTAGTAGGCAAGCTCATTTATCTCTCACATACACGACCTGATATTGCATATGCTGTAAGCGTTGTAAGTCAATTCATGCATGCACCAAGTGAAGATCACATGCACGCGGTACATAGAATTCTGAGGTATTTGAAAACAAATCCCGGTAAAGGGATCATGTTTTCCAAGAATAACCACACAGACATAGCTGGATACACAGATGCAGATTGGGCTGGTTCTATCACTGATAGAAGGTCTACTTCTGAAGCAAAAAGTGGTTGCTAG

Protein Analysis

1051

Amino Acids

118.66

Weight (kDa)

8.46

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 63 - 137 9.4e-17 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 168 - 240 8.3e-16 GAG-pre-integrase domain
rve PF00665 255 - 353 5e-13 Integrase core domain
SH3_retrovirus PF25597 440 - 496 7.8e-25 Retroviral polymerase SH3-like domain
RVT_2 PF07727 700 - 943 1e-69 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2529
AccB7I CCANNNNNTGG 1 cut(s) 2487
AccI GTMKAC 4 cut(s) 1214, 2225, 2388, 3128
AccII CGCG 1 cut(s) 2999
AciI CCGC 8 cut(s) 20, 23, 484, 684, 1076, 1090, 2312, 2999
AclWI GGATC 8 cut(s) 875, 922, 1295, 1772, 1907, 2162, 2403, 3053
AcoI YGGCCR 1 cut(s) 2271
AcsI RAATTY 3 cut(s) 1808, 1825, 3009
AcuI CTGAAG 6 cut(s) 114, 237, 1193, 1496, 2354, 3156
AdeI CACNNNGTG 1 cut(s) 2981
AfaI GTAC 8 cut(s) 1332, 1447, 1603, 2028, 2171, 2185, 2295, 3003
AfiI CCNNNNNNNGG 6 cut(s) 305, 469, 632, 2487, 3041, 3042
AflIII ACRYGT 2 cut(s) 1043, 1950
AgeI ACCGGT 2 cut(s) 261, 2266
AhdI GACNNNNNGTC 1 cut(s) 1044
AhlI ACTAGT 1 cut(s) 2110
AjnI CCWGG 4 cut(s) 62, 2204, 2404, 2480
AjuI GAANNNNNNNTTGG 2 cut(s) 2693, 2725
Alw21I GWGCWC 2 cut(s) 746, 1132
Alw26I GTCTC 3 cut(s) 2249, 2364, 2780
AlwI GGATC 8 cut(s) 875, 922, 1295, 1772, 1907, 2162, 2403, 3053
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 3 cut(s) 1926, 2271, 2327
ApeKI GCWGC 2 cut(s) 1699, 2174
ApoI RAATTY 3 cut(s) 1808, 1825, 3009
AseI ATTAAT 1 cut(s) 1791
AsiGI ACCGGT 2 cut(s) 261, 2266
Asp700I GAANNNNTTC 1 cut(s) 2705
AspS9I GGNCC 2 cut(s) 211, 794
AsuC2I CCSGG 2 cut(s) 856, 3036
AsuHPI GGTGA 6 cut(s) 51, 233, 251, 713, 995, 1707
AsuII TTCGAA 1 cut(s) 1873
AvaII GGWCC 2 cut(s) 211, 794
AxyI CCTNAGG 1 cut(s) 1770
BalI TGGCCA 1 cut(s) 2273
BanII GRGCYC 2 cut(s) 746, 1132
BbsI GAAGAC 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 746, 1132
BbvI GCAGC 2 cut(s) 1686, 2186
BccI CCATC 9 cut(s) 634, 688, 1002, 1169, 1347, 1759, 2145, 2174, 2818
BciT130I CCWGG 4 cut(s) 64, 2206, 2406, 2482
BciVI GTATCC 1 cut(s) 3077
BcnI CCSGG 2 cut(s) 856, 3036
BcoDI GTCTC 3 cut(s) 2249, 2364, 2780
BcuI ACTAGT 1 cut(s) 2110
BfuI GTATCC 1 cut(s) 3077
BglII AGATCT 1 cut(s) 2686
BisI GCNGC 4 cut(s) 23, 1700, 2175, 2313
BlsI GCNGC 4 cut(s) 24, 1701, 2176, 2314
BmcAI AGTACT 2 cut(s) 1603, 2295
Bme1390I CCNGG 6 cut(s) 64, 856, 2206, 2406, 2482, 3036
Bme18I GGWCC 2 cut(s) 211, 794
BmeRI GACNNNNNGTC 1 cut(s) 1044
BmgT120I GGNCC 2 cut(s) 211, 794
BmiI GGNNCC 3 cut(s) 795, 1575, 1851
BmrFI CCNGG 6 cut(s) 64, 856, 2206, 2406, 2482, 3036
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 6 cut(s) 565, 1035, 1147, 1354, 2023, 3082
BmuI ACTGGG 1 cut(s) 896
BpiI GAAGAC 1 cut(s) 1536
BpmI CTGGAG 2 cut(s) 1626, 1692
Bpu14I TTCGAA 1 cut(s) 1873
BpuEI CTTGAG 1 cut(s) 1185
BpuMI CCSGG 2 cut(s) 856, 3036
BsaBI GATNNNNATC 1 cut(s) 294
BsaJI CCNNGG 9 cut(s) 62, 437, 1021, 1458, 1569, 1578, 1923, 2480, 2481
BsaWI WCCGGW 2 cut(s) 261, 2266
BsaXI ACNNNNNCTCC 4 cut(s) 972, 1002, 1749, 1779
Bsc4I CCNNNNNNNGG 6 cut(s) 305, 469, 632, 2487, 3041, 3042
Bse118I RCCGGY 2 cut(s) 261, 2266
Bse1I ACTGG 9 cut(s) 902, 1006, 1230, 1266, 1609, 1648, 1718, 2330, 2794
Bse21I CCTNAGG 1 cut(s) 1770
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 4 cut(s) 64, 2206, 2406, 2482
BseDI CCNNGG 9 cut(s) 62, 437, 1021, 1458, 1569, 1578, 1923, 2480, 2481
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 6 cut(s) 305, 469, 632, 2487, 3041, 3042
BseMII CTCAG 5 cut(s) 950, 1761, 1821, 2243, 3005
BseNI ACTGG 9 cut(s) 902, 1006, 1230, 1266, 1609, 1648, 1718, 2330, 2794
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 2 cut(s) 1686, 2186
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 2 cut(s) 2167, 2571
Bsh1236I CGCG 1 cut(s) 2999
BshFI GGCC 3 cut(s) 1928, 2273, 2329
BshTI ACCGGT 2 cut(s) 261, 2266
BsiHKAI GWGCWC 2 cut(s) 746, 1132
BsiSI CCGG 4 cut(s) 262, 856, 2267, 3036
BslFI GGGAC 3 cut(s) 633, 807, 2461
BslI CCNNNNNNNGG 6 cut(s) 305, 469, 632, 2487, 3041, 3042
BsmAI GTCTC 3 cut(s) 2249, 2364, 2780
BsmFI GGGAC 3 cut(s) 633, 807, 2461
BsnI GGCC 3 cut(s) 1928, 2273, 2329
Bsp119I TTCGAA 1 cut(s) 1873
Bsp1286I GDGCHC 2 cut(s) 746, 1132
Bsp1407I TGTACA 1 cut(s) 1330
Bsp19I CCATGG 3 cut(s) 1021, 1458, 1578
BspACI CCGC 8 cut(s) 20, 23, 484, 684, 1076, 1090, 2312, 2999
BspANI GGCC 3 cut(s) 1928, 2273, 2329
BspCNI CTCAG 5 cut(s) 949, 1762, 1822, 2242, 3006
BspFNI CGCG 1 cut(s) 2999
BspHI TCATGA 2 cut(s) 120, 1813
BspLI GGNNCC 3 cut(s) 795, 1575, 1851
BspPI GGATC 8 cut(s) 875, 922, 1295, 1772, 1907, 2162, 2403, 3053
BspQI GCTCTTC 1 cut(s) 1530
BspT104I TTCGAA 1 cut(s) 1873
BsrFI RCCGGY 2 cut(s) 261, 2266
BsrGI TGTACA 1 cut(s) 1330
BsrI ACTGG 9 cut(s) 902, 1006, 1230, 1266, 1609, 1648, 1718, 2330, 2794
BssAI RCCGGY 2 cut(s) 261, 2266
BssECI CCNNGG 9 cut(s) 62, 437, 1021, 1458, 1569, 1578, 1923, 2480, 2481
BssNAI GTATAC 1 cut(s) 2226
BssT1I CCWWGG 5 cut(s) 1021, 1458, 1569, 1578, 1923
Bst1107I GTATAC 1 cut(s) 2226
Bst2UI CCWGG 4 cut(s) 64, 2206, 2406, 2482
Bst4CI ACNGT 8 cut(s) 352, 725, 1174, 2287, 2301, 2475, 2520, 2556
Bst6I CTCTTC 4 cut(s) 656, 1515, 1530, 2064
BstAUI TGTACA 1 cut(s) 1330
BstBI TTCGAA 1 cut(s) 1873
BstC8I GCNNGC 5 cut(s) 532, 1111, 2903, 2973, 2997
BstDEI CTNAG 7 cut(s) 936, 1221, 1356, 1770, 1830, 2229, 3014
BstDSI CCRYGG 4 cut(s) 437, 1021, 1458, 1578
BstFNI CGCG 1 cut(s) 2999
BstMAI GTCTC 3 cut(s) 2249, 2364, 2780
BstMWI GCNNNNNNNGC 5 cut(s) 702, 711, 2270, 2335, 3101
BstNI CCWGG 4 cut(s) 64, 2206, 2406, 2482
BstNSI RCATGY 6 cut(s) 29, 1047, 1954, 2805, 2975, 2995
BstSCI CCNGG 6 cut(s) 62, 854, 2204, 2404, 2480, 3034
BstUI CGCG 1 cut(s) 2999
BstV1I GCAGC 2 cut(s) 1686, 2186
BstV2I GAAGAC 1 cut(s) 1536
BstX2I RGATCY 5 cut(s) 867, 1912, 2154, 2395, 2686
BstXI CCANNNNNNTGG 1 cut(s) 1595
BstYI RGATCY 5 cut(s) 867, 1912, 2154, 2395, 2686
BstZ17I GTATAC 1 cut(s) 2226
Bsu36I CCTNAGG 1 cut(s) 1770
BsuI GTATCC 1 cut(s) 3077
BsuRI GGCC 3 cut(s) 1928, 2273, 2329
BtgI CCRYGG 4 cut(s) 437, 1021, 1458, 1578
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 5 cut(s) 357, 525, 1839, 2552, 3114
Cac8I GCNNGC 5 cut(s) 532, 1111, 2903, 2973, 2997
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 2 cut(s) 120, 1813
Cfr10I RCCGGY 2 cut(s) 261, 2266
Cfr13I GGNCC 2 cut(s) 211, 794
CseI GACGC 1 cut(s) 1971
Csp6I GTAC 8 cut(s) 1331, 1446, 1602, 2027, 2170, 2184, 2294, 3002
CspAI ACCGGT 2 cut(s) 261, 2266
CviQI GTAC 8 cut(s) 1331, 1446, 1602, 2027, 2170, 2184, 2294, 3002
DdeI CTNAG 7 cut(s) 936, 1221, 1356, 1770, 1830, 2229, 3014
DraI TTTAAA 1 cut(s) 691
DraIII CACNNNGTG 1 cut(s) 2981
DriI GACNNNNNGTC 1 cut(s) 1044
EaeI YGGCCR 1 cut(s) 2271
Eam1104I CTCTTC 4 cut(s) 656, 1515, 1530, 2064
Eam1105I GACNNNNNGTC 1 cut(s) 1044
EarI CTCTTC 4 cut(s) 656, 1515, 1530, 2064
EciI GGCGGA 2 cut(s) 673, 1091
Ecl136II GAGCTC 2 cut(s) 744, 1130
Eco130I CCWWGG 5 cut(s) 1021, 1458, 1569, 1578, 1923
Eco24I GRGCYC 2 cut(s) 746, 1132
Eco32I GATATC 2 cut(s) 1418, 2885
Eco47I GGWCC 2 cut(s) 211, 794
Eco53kI GAGCTC 2 cut(s) 744, 1130
Eco57I CTGAAG 6 cut(s) 114, 237, 1193, 1496, 2354, 3156
Eco81I CCTNAGG 1 cut(s) 1770
EcoICRI GAGCTC 2 cut(s) 744, 1130
EcoO109I RGGNCCY 1 cut(s) 794
EcoRI GAATTC 1 cut(s) 3009
EcoRII CCWGG 4 cut(s) 62, 2204, 2404, 2480
EcoRV GATATC 2 cut(s) 1418, 2885
EcoT14I CCWWGG 5 cut(s) 1021, 1458, 1569, 1578, 1923
EcoT22I ATGCAT 1 cut(s) 2973
EcoT38I GRGCYC 2 cut(s) 746, 1132
ErhI CCWWGG 5 cut(s) 1021, 1458, 1569, 1578, 1923
FalI AAGNNNNNCTT 6 cut(s) 1370, 1402, 2565, 2597, 3115, 3147
FaqI GGGAC 3 cut(s) 633, 807, 2461
FauI CCCGC 1 cut(s) 477
FauNDI CATATG 5 cut(s) 216, 1134, 1978, 2236, 2941
FblI GTMKAC 4 cut(s) 1214, 2225, 2388, 3128
Fnu4HI GCNGC 4 cut(s) 23, 1700, 2175, 2313
FriOI GRGCYC 2 cut(s) 746, 1132
Fsp4HI GCNGC 4 cut(s) 23, 1700, 2175, 2313
GluI GCNGC 4 cut(s) 23, 1700, 2175, 2313
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 2 cut(s) 1626, 1692
HaeIII GGCC 3 cut(s) 1928, 2273, 2329
HapII CCGG 4 cut(s) 262, 856, 2267, 3036
HgaI GACGC 1 cut(s) 1971
HincII GTYRAC 3 cut(s) 1889, 2773, 2807
HindII GTYRAC 3 cut(s) 1889, 2773, 2807
HpaII CCGG 4 cut(s) 262, 856, 2267, 3036
HphI GGTGA 6 cut(s) 51, 233, 251, 713, 995, 1707
HpyCH4III ACNGT 8 cut(s) 352, 725, 1174, 2287, 2301, 2475, 2520, 2556
HpyCH4IV ACGT 1 cut(s) 1444
HpyF10VI GCNNNNNNNGC 5 cut(s) 702, 711, 2270, 2335, 3101
HpyF3I CTNAG 7 cut(s) 936, 1221, 1356, 1770, 1830, 2229, 3014
HpySE526I ACGT 1 cut(s) 1444
LguI GCTCTTC 1 cut(s) 1530
LmnI GCTCC 6 cut(s) 749, 1118, 1127, 1641, 1770, 2827
Lsp1109I GCAGC 2 cut(s) 1686, 2186
LweI GCATC 6 cut(s) 565, 1035, 1147, 1354, 2023, 3082
MaeII ACGT 1 cut(s) 1444
MflI RGATCY 5 cut(s) 867, 1912, 2154, 2395, 2686
MhlI GDGCHC 2 cut(s) 746, 1132
MlsI TGGCCA 1 cut(s) 2273
MluNI TGGCCA 1 cut(s) 2273
MlyI GAGTC 3 cut(s) 326, 727, 2104
MmeI TCCRAC 3 cut(s) 845, 1341, 1457
Mox20I TGGCCA 1 cut(s) 2273
Mph1103I ATGCAT 1 cut(s) 2973
MroXI GAANNNNTTC 1 cut(s) 2705
MscI TGGCCA 1 cut(s) 2273
MseI TTAA 5 cut(s) 171, 248, 690, 954, 1791
MslI CAYNNNNRTG 5 cut(s) 518, 783, 857, 1479, 2720
Msp20I TGGCCA 1 cut(s) 2273
MspA1I CMGCKG 2 cut(s) 1699, 2177
MspI CCGG 4 cut(s) 262, 856, 2267, 3036
MspR9I CCNGG 6 cut(s) 64, 856, 2206, 2406, 2482, 3036
MvaI CCWGG 4 cut(s) 64, 2206, 2406, 2482
MvnI CGCG 1 cut(s) 2999
MwoI GCNNNNNNNGC 5 cut(s) 702, 711, 2270, 2335, 3101
NciI CCSGG 2 cut(s) 856, 3036
NcoI CCATGG 3 cut(s) 1021, 1458, 1578
NdeI CATATG 5 cut(s) 216, 1134, 1978, 2236, 2941
NlaIV GGNNCC 3 cut(s) 795, 1575, 1851
NmuCI GTSAC 3 cut(s) 319, 719, 2617
NsiI ATGCAT 1 cut(s) 2973
NspI RCATGY 6 cut(s) 29, 1047, 1954, 2805, 2975, 2995
NspV TTCGAA 1 cut(s) 1873
PaeI GCATGC 1 cut(s) 2975
PagI TCATGA 2 cut(s) 120, 1813
PasI CCCWGGG 1 cut(s) 2481
PciI ACATGT 2 cut(s) 1043, 1950
PciSI GCTCTTC 1 cut(s) 1530
PdmI GAANNNNTTC 1 cut(s) 2705
PfeI GAWTC 8 cut(s) 91, 197, 290, 562, 1142, 1677, 1712, 2499
PflFI GACNNNGTC 1 cut(s) 798
PflMI CCANNNNNTGG 1 cut(s) 2487
PinAI ACCGGT 2 cut(s) 261, 2266
PkrI GCNGC 4 cut(s) 24, 1701, 2176, 2314
PleI GAGTC 3 cut(s) 326, 726, 2103
PpsI GAGTC 3 cut(s) 326, 726, 2103
PpuMI RGGWCCY 1 cut(s) 794
PscI ACATGT 2 cut(s) 1043, 1950
PshBI ATTAAT 1 cut(s) 1791
PsiI TTATAA 1 cut(s) 2529
Psp124BI GAGCTC 2 cut(s) 746, 1132
Psp5II RGGWCCY 1 cut(s) 794
Psp6I CCWGG 4 cut(s) 62, 2204, 2404, 2480
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 4 cut(s) 62, 2204, 2404, 2480
PspN4I GGNNCC 3 cut(s) 795, 1575, 1851
PspPI GGNCC 2 cut(s) 211, 794
PspPPI RGGWCCY 1 cut(s) 794
PsrI GAACNNNNNNTAC 2 cut(s) 1470, 1502
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 5 cut(s) 867, 1912, 2154, 2395, 2686
PsyI GACNNNGTC 1 cut(s) 798
PvuII CAGCTG 2 cut(s) 1699, 2177
RsaI GTAC 8 cut(s) 1332, 1447, 1603, 2028, 2171, 2185, 2295, 3003
RsaNI GTAC 8 cut(s) 1331, 1446, 1602, 2027, 2170, 2184, 2294, 3002
RseI CAYNNNNRTG 5 cut(s) 518, 783, 857, 1479, 2720
SacI GAGCTC 2 cut(s) 746, 1132
SapI GCTCTTC 1 cut(s) 1530
SaqAI TTAA 5 cut(s) 171, 248, 690, 954, 1791
SatI GCNGC 4 cut(s) 23, 1700, 2175, 2313
Sau96I GGNCC 2 cut(s) 211, 794
ScaI AGTACT 2 cut(s) 1603, 2295
SchI GAGTC 3 cut(s) 326, 727, 2104
ScrFI CCNGG 6 cut(s) 64, 856, 2206, 2406, 2482, 3036
SduI GDGCHC 2 cut(s) 746, 1132
SfaNI GCATC 6 cut(s) 565, 1035, 1147, 1354, 2023, 3082
SfuI TTCGAA 1 cut(s) 1873
SgrAI CRCCGGYG 1 cut(s) 2266
SinI GGWCC 2 cut(s) 211, 794
SmiMI CAYNNNNRTG 5 cut(s) 518, 783, 857, 1479, 2720
SmlI CTYRAG 1 cut(s) 1200
SmoI CTYRAG 1 cut(s) 1200
SpeI ACTAGT 1 cut(s) 2110
SphI GCATGC 1 cut(s) 2975
SsiI CCGC 8 cut(s) 20, 23, 484, 684, 1076, 1090, 2312, 2999
SspI AATATT 1 cut(s) 238
SstI GAGCTC 2 cut(s) 746, 1132
StyD4I CCNGG 6 cut(s) 62, 854, 2204, 2404, 2480, 3034
StyI CCWWGG 5 cut(s) 1021, 1458, 1569, 1578, 1923
TaaI ACNGT 8 cut(s) 352, 725, 1174, 2287, 2301, 2475, 2520, 2556
TaiI ACGT 1 cut(s) 1447
TaqI TCGA 7 cut(s) 10, 155, 843, 1342, 1547, 1873, 2290
TatI WGTACW 4 cut(s) 1330, 1601, 2026, 2293
TauI GCSGC 2 cut(s) 25, 2315
TfiI GAWTC 8 cut(s) 91, 197, 290, 562, 1142, 1677, 1712, 2499
Tru1I TTAA 5 cut(s) 171, 248, 690, 954, 1791
Tru9I TTAA 5 cut(s) 171, 248, 690, 954, 1791
TscAI CASTG 5 cut(s) 357, 532, 1846, 2559, 3121
TseFI GTSAC 3 cut(s) 319, 719, 2617
TseI GCWGC 2 cut(s) 1699, 2174
Tsp45I GTSAC 3 cut(s) 319, 719, 2617
TspGWI ACGGA 2 cut(s) 224, 1862
TspRI CASTG 5 cut(s) 357, 532, 1846, 2559, 3121
Tth111I GACNNNGTC 1 cut(s) 798
Van91I CCANNNNNTGG 1 cut(s) 2487
VpaK11BI GGWCC 2 cut(s) 211, 794
VspI ATTAAT 1 cut(s) 1791
XapI RAATTY 3 cut(s) 1808, 1825, 3009
XbaI TCTAGA 1 cut(s) 1673
XceI RCATGY 6 cut(s) 29, 1047, 1954, 2805, 2975, 2995
XcmI CCANNNNNNNNNTGG 1 cut(s) 1146
XmiI GTMKAC 4 cut(s) 1214, 2225, 2388, 3128
XmnI GAANNNNTTC 1 cut(s) 2705
ZrmI AGTACT 2 cut(s) 1603, 2295
Zsp2I ATGCAT 1 cut(s) 2973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.