RchiOBHm_Chr5g0066601

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000071
Physical Location & Seq
Forward (+)
72715920 .. 72718266
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34236

Sequence Viewer

Length: 2082 bp
ATGGGGAACGACGACTATGTCGGTGGTCTGGCGAAGGGAAACGACGGACGGCCGGGAATGGAGGAACGACCGCCATTGGACGACGCCGGCGATCTGGAGACGGGCGTGAACTGGACGACGCCGTTTCAAGACAGGCGCAAACTGGACGACGCCGGCGATCTGGAGACGGGGAACGGCGATGACGTCGTTTGCGGTGACCTGGAGAACGCCACCATTTTGGATTATGGACAGAACGATGTGGTTTCAGATCAAGGACAGAACGATGTCGTTTCAGACATCAGTGAACCCGACTTGGAAGTCAAAACTTCTCCCTCTCCTTCATCTCCCCCCTCGAGCCAAATTCTCCAAGGTCCTGAAGGTCAAGAGGTAATACCTGAACCCAATTTGTCTGTTTTACCTTCTCGTTCTACTCGTGGTAAACCACCAAAGAAATATGAACCTAGTCTTAAAGCTAAGTCTACGTACCCGGTTGCTAATTACGTATCTACACATAGGTTGTCCAAGTCATATGAGTCGTTTGTGCATCAGATTTCCTCTGTATCTATTCCTAACAAAGTGCAGGATGCCCTAAGAGATCCAAAGTGGTCAAAAGCGATGGAAGAAGAGATGGAAGCTCTACAGAAGAATAACACTTGGGAGTTGATAGGGTTACCTAAAGGAAAAAAAACAGTCGGATGTAAATGGGTCTACACAATTAAACATGATGCAGATGGCTCAGTTAGTAGGTACAAGGCGAGATTGGTAGCAAAAGGGTTTACACAGACGTATGGTGTAGACTATGATGAGACGTTTGCACCCGTAGCCAAGATCAATACTATCAGAGTGCTTCTCTCTTTGGCGGCTAATCTGGACTGGCCCCTCAAGCAACTTGATGTGAAAAATGCTTTTCTACATGGAGATTTAGCTGAAGAAGTGTATATGAGCCTACCTCCTGGATATGAGACCACTAATAAAACGGATGTTGTGTGTAGGTTGAAGAAGTCACTCTATGGTTTGAAACAATCCCCCCGTGCGTGGTTTGGGCGCTTTACTCAGGCAATGAAGCGATTTGGTTACAAACAAAGCAACTCGGATCATACTCTCTTTCTTAAACATCAAAAGGGTAAAGTAACGGTGTTAATCATCTATGTTGATGATATGGTAATTACAGGTGATGATCGAGAGGAAATTAGGAGGTTGCAGCAGCAGTTAGCTTCAGAGTTTGAGATGAAAGACCTTGGTGACTTGAGATACTTTCTTGGGATAGAGGTAGCCAGGGGGAGTAGCAGCATCTTCTTGTGCCAACGAAAATATGTTTTAGACTTGCTGTCAGAAACTGGCATGTTAGATTGCAGACCAGCTGATACACCAATTGAACAGAATCATCGGTTAGCGGAGTACCCTGATCAAGTCCCGACTGATAGGGCTCGGTATCAAAGGTTAGTTGGCAAATTAATCTATTTGGGTCACACTAGACCTGATTTGGCATATGCAGTAAGTGTGGTGAGCCAGTTCATGCATAACCCAAGTGAAGACCATATGGAAGCAGTCGTGCGAATCCTGCGGTATCTAAAGCGTGCTCCTGGGAAGGGCCTTATGTTCTCAAAACATCACTACCTAGAGGTGAGTGGTTATACGGATGCTGATTGGGCTGGTTGTATCACAGATAGGAGGTCAACCTCGGGTTACTTTACATTTGTTGGAGGGAACCTTGTCACTTGGAAGAGTAAAAAACAAAAGGTGGTGGCGAGGTCTAGTGCAGAAGCCGAGTATAGAGGTATGGCACATGGAGTATGTGAGTTGTTATGGTTACGTAATTTATTGCGTGATTTGGGGTTCAAGCCAAAAAGAGCTATGGAGTTGTTTTGTGATAACAAAGCAGCTATTGAAATTTCACATAACCCGGTGCAGCATGATCGTACAAAGCATGTGGAAGTTGACCGGCATTTTATCAAGGAGAAGTTGGACGCCAACCTAATTGCCTTCCCATTTGTACCAACTGAGGAGCAGTTAGCAGATGTTCTGACTAAGGGTGTGTCAGTCAAAGCATTTCATGACTCACTTGACAAGTTGGGCATTCGAGATGTGTATGCTCCAACTTGA

Protein Analysis

693

Amino Acids

77.93

Weight (kDa)

6.44

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 209 - 452 4e-79 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 17, 296
AatII GACGTC 1 cut(s) 186
AccI GTMKAC 3 cut(s) 458, 687, 774
AciI CCGC 5 cut(s) 71, 192, 839, 1373, 1543
AclWI GGATC 2 cut(s) 569, 1080
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 2 cut(s) 339, 1869
AcuI CTGAAG 3 cut(s) 375, 927, 1179
AcyI GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
AfaI GTAC 5 cut(s) 464, 728, 1379, 1900, 1974
AfiI CCNNNNNNNGG 2 cut(s) 1014, 1568
AgsI TTSAA 6 cut(s) 128, 976, 997, 1355, 1819, 1868
AhdI GACNNNNNGTC 1 cut(s) 1306
AjnI CCWGG 4 cut(s) 198, 931, 1253, 1561
AluBI AGCT 7 cut(s) 452, 614, 905, 1193, 1340, 1832, 1862
AluI AGCT 7 cut(s) 452, 614, 905, 1193, 1340, 1832, 1862
Alw21I GWGCWC 1 cut(s) 1561
Alw26I GTCTC 4 cut(s) 92, 158, 779, 935
AlwI GGATC 2 cut(s) 569, 1080
AlwNI CAGNNNCTG 1 cut(s) 1316
Ama87I CYCGRG 2 cut(s) 331, 1660
AoxI GGCC 3 cut(s) 50, 854, 1570
ApeKI GCWGC 5 cut(s) 1180, 1183, 1266, 1859, 1888
ApoI RAATTY 2 cut(s) 339, 1869
AseI ATTAAT 1 cut(s) 1433
AspLEI GCGC 2 cut(s) 138, 1026
AspS9I GGNCC 3 cut(s) 350, 855, 1570
AsuC2I CCSGG 3 cut(s) 54, 467, 1883
AsuHPI GGTGA 5 cut(s) 206, 1163, 1232, 1495, 1615
AvaI CYCGRG 2 cut(s) 331, 1660
AvaII GGWCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 1408
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 1518
Bbv12I GWGCWC 1 cut(s) 1561
BbvI GCAGC 5 cut(s) 1192, 1195, 1278, 1871, 1900
BccI CCATC 3 cut(s) 589, 601, 704
BceAI ACGGC 3 cut(s) 65, 106, 190
BcgI CGANNNNNNTGC 2 cut(s) 1877, 1911
BciT130I CCWGG 4 cut(s) 200, 933, 1255, 1563
BclI TGATCA 1 cut(s) 1384
BcnI CCSGG 3 cut(s) 54, 467, 1883
BcoDI GTCTC 4 cut(s) 92, 158, 779, 935
BfaI CTAG 4 cut(s) 441, 1452, 1598, 1734
BfmI CTRYAG 1 cut(s) 617
BfoI RGCGCY 1 cut(s) 1027
BisI GCNGC 6 cut(s) 840, 1181, 1184, 1267, 1860, 1889
BlsI GCNGC 6 cut(s) 841, 1182, 1185, 1268, 1861, 1890
Bme1390I CCNGG 7 cut(s) 54, 200, 467, 933, 1255, 1563, 1883
Bme18I GGWCC 1 cut(s) 350
BmeRI GACNNNNNGTC 1 cut(s) 1306
BmeT110I CYCGRG 2 cut(s) 331, 1660
BmgT120I GGNCC 3 cut(s) 350, 855, 1570
BmiI GGNNCC 2 cut(s) 857, 1688
BmrFI CCNGG 7 cut(s) 54, 200, 467, 933, 1255, 1563, 1883
BmsI GCATC 5 cut(s) 532, 553, 694, 1278, 1609
BpiI GAAGAC 1 cut(s) 1518
BplI GAGNNNNNCTC 4 cut(s) 813, 845, 913, 945
BpmI CTGGAG 3 cut(s) 116, 182, 221
BpuEI CTTGAG 2 cut(s) 845, 1246
BpuMI CCSGG 3 cut(s) 54, 467, 1883
BsaAI YACGTR 3 cut(s) 462, 481, 1793
BsaHI GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
BsaI GGTCTC 1 cut(s) 935
BsaJI CCNNGG 5 cut(s) 346, 1216, 1254, 1562, 1659
Bsc4I CCNNNNNNNGG 2 cut(s) 1014, 1568
Bse118I RCCGGY 3 cut(s) 86, 152, 1920
Bse1I ACTGG 5 cut(s) 116, 147, 857, 1321, 1489
Bse3DI GCAATG 1 cut(s) 1044
BseBI CCWGG 4 cut(s) 200, 933, 1255, 1563
BseDI CCNNGG 5 cut(s) 346, 1216, 1254, 1562, 1659
BseGI GGATG 4 cut(s) 568, 680, 964, 1624
BseLI CCNNNNNNNGG 2 cut(s) 1014, 1568
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 3 cut(s) 729, 1046, 1971
BseNI ACTGG 5 cut(s) 116, 147, 857, 1321, 1489
BseRI GAGGAG 1 cut(s) 1997
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 5 cut(s) 1192, 1195, 1278, 1871, 1900
BsgI GTGCAG 3 cut(s) 578, 1758, 1907
Bsh1285I CGRYCG 2 cut(s) 53, 71
BshFI GGCC 3 cut(s) 52, 856, 1572
BsiEI CGRYCG 2 cut(s) 53, 71
BsiHKAI GWGCWC 1 cut(s) 1561
BsiHKCI CYCGRG 2 cut(s) 331, 1660
BsiSI CCGG 6 cut(s) 53, 87, 153, 467, 1883, 1921
BslFI GGGAC 1 cut(s) 1376
BslI CCNNNNNNNGG 2 cut(s) 1014, 1568
BsmAI GTCTC 4 cut(s) 92, 158, 779, 935
BsmBI CGTCTC 3 cut(s) 92, 158, 779
BsmFI GGGAC 1 cut(s) 1376
BsmI GAATGC 1 cut(s) 2055
BsnI GGCC 3 cut(s) 52, 856, 1572
Bso31I GGTCTC 1 cut(s) 935
BsoBI CYCGRG 2 cut(s) 331, 1660
Bsp1286I GDGCHC 2 cut(s) 1408, 1561
Bsp143I GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
BspACI CCGC 5 cut(s) 71, 192, 839, 1373, 1543
BspANI GGCC 3 cut(s) 52, 856, 1572
BspCNI CTCAG 3 cut(s) 728, 1045, 1972
BspHI TCATGA 1 cut(s) 2032
BspLI GGNNCC 2 cut(s) 857, 1688
BspPI GGATC 2 cut(s) 569, 1080
BspTNI GGTCTC 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 3 cut(s) 86, 152, 1920
BsrI ACTGG 5 cut(s) 116, 147, 857, 1321, 1489
BssAI RCCGGY 3 cut(s) 86, 152, 1920
BssECI CCNNGG 5 cut(s) 346, 1216, 1254, 1562, 1659
BssMI GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
BssNI GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 2 cut(s) 346, 1216
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 4 cut(s) 200, 933, 1255, 1563
Bst4CI ACNGT 2 cut(s) 670, 1114
Bst6I CTCTTC 2 cut(s) 597, 1697
BstACI GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
BstBAI YACGTR 3 cut(s) 462, 481, 1793
BstC8I GCNNGC 3 cut(s) 88, 154, 1557
BstDEI CTNAG 6 cut(s) 453, 569, 715, 1032, 1980, 2007
BstEII GGTNACC 2 cut(s) 194, 648
BstF5I GGATG 4 cut(s) 568, 680, 964, 1624
BstH2I RGCGCY 1 cut(s) 1027
BstHHI GCGC 2 cut(s) 138, 1026
BstKTI GATC 9 cut(s) 94, 160, 250, 577, 810, 1075, 1159, 1387, 1897
BstMAI GTCTC 4 cut(s) 92, 158, 779, 935
BstMBI GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
BstMCI CGRYCG 2 cut(s) 53, 71
BstMWI GCNNNNNNNGC 4 cut(s) 800, 862, 1540, 1628
BstNI CCWGG 4 cut(s) 200, 933, 1255, 1563
BstNSI RCATGY 2 cut(s) 1324, 1910
BstPI GGTNACC 2 cut(s) 194, 648
BstSCI CCNGG 7 cut(s) 52, 198, 465, 931, 1253, 1561, 1881
BstSFI CTRYAG 1 cut(s) 617
BstSNI TACGTA 3 cut(s) 462, 481, 1793
BstV1I GCAGC 5 cut(s) 1192, 1195, 1278, 1871, 1900
BstV2I GAAGAC 1 cut(s) 1518
BstX2I RGATCY 1 cut(s) 574
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 1 cut(s) 574
BstZI CGGCCG 1 cut(s) 50
BsuRI GGCC 3 cut(s) 52, 856, 1572
BtgZI GCGATG 2 cut(s) 192, 608
BtsCI GGATG 4 cut(s) 568, 680, 964, 1624
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 88, 154, 1557
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 1 cut(s) 2032
CfoI GCGC 2 cut(s) 138, 1026
Cfr10I RCCGGY 3 cut(s) 86, 152, 1920
Cfr13I GGNCC 3 cut(s) 350, 855, 1570
CseI GACGC 4 cut(s) 92, 127, 158, 1955
Csp6I GTAC 5 cut(s) 463, 727, 1378, 1899, 1973
CspCI CAANNNNNGTGG 2 cut(s) 1890, 1925
CviAII CATG 8 cut(s) 701, 893, 1321, 1495, 1766, 1892, 1907, 2033
CviQI GTAC 5 cut(s) 463, 727, 1378, 1899, 1973
DdeI CTNAG 6 cut(s) 453, 569, 715, 1032, 1980, 2007
DpnI GATC 9 cut(s) 93, 159, 249, 576, 809, 1074, 1158, 1386, 1896
DpnII GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
DrdI GACNNNNNNGTC 2 cut(s) 17, 296
DriI GACNNNNNGTC 1 cut(s) 1306
DseDI GACNNNNNNGTC 2 cut(s) 17, 296
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 2 cut(s) 597, 1697
Eam1105I GACNNNNNGTC 1 cut(s) 1306
EarI CTCTTC 2 cut(s) 597, 1697
EclXI CGGCCG 1 cut(s) 50
Eco105I TACGTA 3 cut(s) 462, 481, 1793
Eco130I CCWWGG 2 cut(s) 346, 1216
Eco24I GRGCYC 1 cut(s) 1408
Eco31I GGTCTC 1 cut(s) 935
Eco47I GGWCC 1 cut(s) 350
Eco52I CGGCCG 1 cut(s) 50
Eco57I CTGAAG 3 cut(s) 375, 927, 1179
Eco88I CYCGRG 2 cut(s) 331, 1660
Eco91I GGTNACC 2 cut(s) 194, 648
EcoO109I RGGNCCY 2 cut(s) 350, 1570
EcoO65I GGTNACC 2 cut(s) 194, 648
EcoRII CCWGG 4 cut(s) 198, 931, 1253, 1561
EcoT14I CCWWGG 2 cut(s) 346, 1216
EcoT22I ATGCAT 1 cut(s) 1500
EcoT38I GRGCYC 1 cut(s) 1408
ErhI CCWWGG 2 cut(s) 346, 1216
Esp3I CGTCTC 3 cut(s) 92, 158, 779
FaeI CATG 8 cut(s) 704, 896, 1324, 1498, 1769, 1895, 1910, 2036
FaqI GGGAC 1 cut(s) 1376
FatI CATG 8 cut(s) 700, 892, 1320, 1494, 1765, 1891, 1906, 2032
FauNDI CATATG 3 cut(s) 508, 1468, 1518
FbaI TGATCA 1 cut(s) 1384
FblI GTMKAC 3 cut(s) 458, 687, 774
Fnu4HI GCNGC 6 cut(s) 840, 1181, 1184, 1267, 1860, 1889
FokI GGATG 4 cut(s) 575, 687, 971, 1631
FriOI GRGCYC 1 cut(s) 1408
Fsp4HI GCNGC 6 cut(s) 840, 1181, 1184, 1267, 1860, 1889
FspBI CTAG 4 cut(s) 441, 1452, 1598, 1734
GlaI GCGC 2 cut(s) 137, 1025
GluI GCNGC 6 cut(s) 840, 1181, 1184, 1267, 1860, 1889
GsuI CTGGAG 3 cut(s) 116, 182, 221
HaeII RGCGCY 1 cut(s) 1027
HaeIII GGCC 3 cut(s) 52, 856, 1572
HapII CCGG 6 cut(s) 53, 87, 153, 467, 1883, 1921
HgaI GACGC 4 cut(s) 92, 127, 158, 1955
HhaI GCGC 2 cut(s) 138, 1026
Hin1I GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
Hin1II CATG 8 cut(s) 704, 896, 1324, 1498, 1769, 1895, 1910, 2036
Hin6I GCGC 2 cut(s) 136, 1024
HinP1I GCGC 2 cut(s) 136, 1024
HincII GTYRAC 2 cut(s) 1656, 1918
HindII GTYRAC 2 cut(s) 1656, 1918
HinfI GANTC 4 cut(s) 512, 1360, 1536, 2036
HpaII CCGG 6 cut(s) 53, 87, 153, 467, 1883, 1921
HphI GGTGA 5 cut(s) 206, 1163, 1232, 1495, 1615
Hpy166II GTNNAC 9 cut(s) 109, 284, 419, 459, 688, 756, 775, 1656, 1918
Hpy188I TCNGA 9 cut(s) 247, 274, 528, 674, 821, 1072, 1198, 1312, 2004
Hpy8I GTNNAC 9 cut(s) 109, 284, 419, 459, 688, 756, 775, 1656, 1918
Hpy99I CGWCG 6 cut(s) 14, 47, 86, 121, 152, 188
HpyAV CCTTC 6 cut(s) 28, 327, 350, 408, 1561, 1972
HpyCH4III ACNGT 2 cut(s) 670, 1114
HpyCH4IV ACGT 6 cut(s) 183, 461, 480, 764, 788, 1792
HpyF10VI GCNNNNNNNGC 4 cut(s) 800, 862, 1540, 1628
HpyF3I CTNAG 6 cut(s) 453, 569, 715, 1032, 1980, 2007
HpySE526I ACGT 6 cut(s) 183, 461, 480, 764, 788, 1792
Hsp92I GRCGYC 5 cut(s) 84, 119, 150, 183, 1947
Hsp92II CATG 8 cut(s) 704, 896, 1324, 1498, 1769, 1895, 1910, 2036
HspAI GCGC 2 cut(s) 136, 1024
KroI GCCGGC 2 cut(s) 86, 152
KroNI GCCGGC 2 cut(s) 88, 154
Ksp22I TGATCA 1 cut(s) 1384
Kzo9I GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
LmnI GCTCC 3 cut(s) 1564, 1984, 2077
Lsp1109I GCAGC 5 cut(s) 1192, 1195, 1278, 1871, 1900
LweI GCATC 5 cut(s) 532, 553, 694, 1278, 1609
MaeI CTAG 4 cut(s) 441, 1452, 1598, 1734
MaeII ACGT 6 cut(s) 183, 461, 480, 764, 788, 1792
MalI GATC 9 cut(s) 93, 159, 249, 576, 809, 1074, 1158, 1386, 1896
MboI GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
MboII GAAGA 8 cut(s) 611, 614, 634, 920, 988, 1264, 1523, 1714
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 2 cut(s) 1408, 1561
MlyI GAGTC 2 cut(s) 521, 2030
MmeI TCCRAC 3 cut(s) 652, 1660, 1923
Mph1103I ATGCAT 1 cut(s) 1500
MreI CGCCGGCG 2 cut(s) 86, 152
MroNI GCCGGC 2 cut(s) 86, 152
MseI TTAA 5 cut(s) 447, 696, 1089, 1118, 1433
MspA1I CMGCKG 1 cut(s) 1340
MspI CCGG 6 cut(s) 53, 87, 153, 467, 1883, 1921
MspR9I CCNGG 7 cut(s) 54, 200, 467, 933, 1255, 1563, 1883
MunI CAATTG 1 cut(s) 1350
Mva1269I GAATGC 1 cut(s) 2055
MvaI CCWGG 4 cut(s) 200, 933, 1255, 1563
MwoI GCNNNNNNNGC 4 cut(s) 800, 862, 1540, 1628
NaeI GCCGGC 2 cut(s) 88, 154
NciI CCSGG 3 cut(s) 54, 467, 1883
NdeI CATATG 3 cut(s) 508, 1468, 1518
NdeII GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
NgoMIV GCCGGC 2 cut(s) 86, 152
NlaIII CATG 8 cut(s) 704, 896, 1324, 1498, 1769, 1895, 1910, 2036
NlaIV GGNNCC 2 cut(s) 857, 1688
NmeAIII GCCGAG 1 cut(s) 1771
NmuCI GTSAC 5 cut(s) 194, 981, 1220, 1445, 1693
NsiI ATGCAT 1 cut(s) 1500
NspI RCATGY 2 cut(s) 1324, 1910
PaeR7I CTCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 2032
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 153, 180, 409
PctI GAATGC 1 cut(s) 2055
PdiI GCCGGC 2 cut(s) 88, 154
PfeI GAWTC 2 cut(s) 1360, 1536
PflFI GACNNNGTC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 931
PkrI GCNGC 6 cut(s) 841, 1182, 1185, 1268, 1861, 1890
PleI GAGTC 2 cut(s) 520, 2030
PpsI GAGTC 2 cut(s) 520, 2030
Ppu21I YACGTR 3 cut(s) 462, 481, 1793
PpuMI RGGWCCY 1 cut(s) 350
PshBI ATTAAT 1 cut(s) 1433
Psp5II RGGWCCY 1 cut(s) 350
Psp6I CCWGG 4 cut(s) 198, 931, 1253, 1561
PspEI GGTNACC 2 cut(s) 194, 648
PspGI CCWGG 4 cut(s) 198, 931, 1253, 1561
PspN4I GGNNCC 2 cut(s) 857, 1688
PspPI GGNCC 3 cut(s) 350, 855, 1570
PspPPI RGGWCCY 1 cut(s) 350
PspXI VCTCGAGB 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 1 cut(s) 574
PsyI GACNNNGTC 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 1340
RsaI GTAC 5 cut(s) 464, 728, 1379, 1900, 1974
RsaNI GTAC 5 cut(s) 463, 727, 1378, 1899, 1973
SaqAI TTAA 5 cut(s) 447, 696, 1089, 1118, 1433
SatI GCNGC 6 cut(s) 840, 1181, 1184, 1267, 1860, 1889
Sau3AI GATC 9 cut(s) 91, 157, 247, 574, 807, 1072, 1156, 1384, 1894
Sau96I GGNCC 3 cut(s) 350, 855, 1570
SchI GAGTC 2 cut(s) 521, 2030
ScrFI CCNGG 7 cut(s) 54, 200, 467, 933, 1255, 1563, 1883
SduI GDGCHC 2 cut(s) 1408, 1561
SfaNI GCATC 5 cut(s) 532, 553, 694, 1278, 1609
SfcI CTRYAG 1 cut(s) 617
Sfr274I CTCGAG 1 cut(s) 331
SgrAI CRCCGGYG 2 cut(s) 86, 152
SinI GGWCC 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 331
SmlI CTYRAG 3 cut(s) 331, 860, 1225
SmoI CTYRAG 3 cut(s) 331, 860, 1225
SnaBI TACGTA 3 cut(s) 462, 481, 1793
SsiI CCGC 5 cut(s) 71, 192, 839, 1373, 1543
SspMI CTAG 4 cut(s) 441, 1452, 1598, 1734
StyD4I CCNGG 7 cut(s) 52, 198, 465, 931, 1253, 1561, 1881
StyI CCWWGG 2 cut(s) 346, 1216
TaaI ACNGT 2 cut(s) 670, 1114
TaiI ACGT 6 cut(s) 186, 464, 483, 767, 791, 1795
TaqI TCGA 3 cut(s) 332, 1159, 2059
TauI GCSGC 1 cut(s) 842
TfiI GAWTC 2 cut(s) 1360, 1536
Tru1I TTAA 5 cut(s) 447, 696, 1089, 1118, 1433
Tru9I TTAA 5 cut(s) 447, 696, 1089, 1118, 1433
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 5 cut(s) 194, 981, 1220, 1445, 1693
TseI GCWGC 5 cut(s) 1180, 1183, 1266, 1859, 1888
Tsp45I GTSAC 5 cut(s) 194, 981, 1220, 1445, 1693
TspDTI ATGAA 6 cut(s) 309, 450, 1055, 1223, 1483, 2021
TspGWI ACGGA 3 cut(s) 60, 971, 1631
TspRI CASTG 1 cut(s) 286
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 350
VspI ATTAAT 1 cut(s) 1433
XapI RAATTY 2 cut(s) 339, 1869
XceI RCATGY 2 cut(s) 1324, 1910
XhoI CTCGAG 1 cut(s) 331
XmiI GTMKAC 3 cut(s) 458, 687, 774
XspI CTAG 4 cut(s) 441, 1452, 1598, 1734
ZraI GACGTC 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.