MD04G1051300.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000071
Physical Location & Seq
Forward (+)
6041116 .. 6045439
4324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1051300.v1.1.491

Sequence Viewer

Length: 3849 bp
ATGGCCACTGACGAAATTCCCAAAGGTTTTGCGGCTGACGTCAAAACTGATGTTTCAAATCCTTTGTTTATTCATCATTCAGATCACCCATCTATGATGCTGGTTTTGAAGCCCCTTAATGGGGACAACTACTCTACCTGGTCTCGTGCAATGAGAATCTCCCTTAGTGCCAAAAACAAGCTAGGTTTTGTTGATGGTACTGTTACACAGCCTTCCGAGAAAACCAAACATGCTGATCATGCTACATGGCAGAAATGCAATGACATGATCGTTGCATGGATTATCAACTCCATTGATTCAGAAATTTCAGACAGTATTCTTTACATGACTGATGCTCGTGCAATCTGGGAGGAGTTGCGTGAGCGTTATTCTCAAAGCAATGCTCTTCGGATTTTCCAATTACAACGGGACATTGCTTCTCTTACTCAAGACCAACTTTCAGTTGCTGCGTACTACACGAAGCTGAAGAAATTATGGGATGAACTGGCGTTATACAGTGACTCCACTTCCTGCACATGTGGAGCGCAGAATGAAAGAAATAAATTAATGCAGTTCCTTATGGGTCTCAACGAGTCCTATAGTGCCATTCGAGGGCAAGTCTTGTTGATGAACCCTTTACCAACTGTTCGTCAGGCCCATGCTTCAATTTCCCAAGAAGAGAAGCAAAGATCTCTTACTGTTTCACGTGGAGTCACCAAGACAGCAGCTATGGCTGTTCGACAAGGTGGTTCATGGTCATCTGGATCTTCAAGTCGTAAGCCCTTGCATTGTACTCATTGTGATCATGACTATCACACCATTGATACTTGTTACCAACTTCACGGGTATCCACCGGGGCATCGTCTTCACAAAGTCAAGCCTCAAAAAGGAAGCAAGTCTTCTAAAAAGGAAGGTAGTTCTTCTTCGTCTGCAAATCAAGTTTCAACAGGTGCAACTTTGGAAGAGATGAAGTCGGTGATGTCCGGGTTATCTGACACTCAATTCCAGCAAATCCTTGACATCATGAATAATAAAGGAGTTGATGTCTCCGTTCCTCCAAAAGCTAATGTCGCTGCCATGAAGACAGGTTTTTCAAAATCTTCTTTGCATTTACGTCGATGGATAATCGACAGTGGTGCAACAGATCATATAACCTCCTCACCTGATTTGCTTACTGATGGTGTCAAAAACACCAATTTGTCACCAGTTTTATTACCAAGTGGAGAGCAAGCTCGCATAGTCTCCACTGGGACAGTTCCTTTGAGTTCCACTTTTTTGTTACGAGATGTATTGTGTGTGCCCTCTTTTCAAGTAGATTTGATGTTTGACTTAACGACGAAGAAGGTGATTGGTTTGGGTAAGCAACGTGGAGGACTTTACTACTTGGTAACGTTGATTCCCACCGTCTCTGGTAGCTCTTACCAAGTCTGCAACCTTGTTCTCTCACCCACAGAGTTGTGGCATCAGCGTTTGGGTCATTTATCTCCTTCTAGGCTCGATTTCATGTCAAAACACTTGTTGCATTTTCCTTTTCAATCCAATAATGCTTGTAATGTTTGTCCTTTAGCCAAGCAAAGTCGTTTACCTTTTAATATCAGTTCCATTACAACCACAAAACCTTTTGATTTGATCCATTGTGATATTTGGGGTCCTTACGTAGTTGCATCAACTTCTGGAGTCAAGTATTTCTTAACTATTGTGGATGATTACTCTCGTTTCACATGGATATTTTTTATGCAACACAAATCAGAAACACAGCACATCCTCAAGGATTTTTTCTCTTTTGTTCAAACTCAATTTAATACATCTATTCGCCAAATACGCGTGGACAATGGTAGAGAATTTTTTTCTCTTCGTGGTTTCTTCCAGGATCATGGGGTCCTTTATCAACGCTCTTGTGTCTATACGCCCCAACAAAATGGGGTCGTCGAGTGCAAGCATCGTCACATACTTGAAATTGCTCGTGCTTTGCGTTTCCAAGCTAACCTTCCTATTACTTTTTGGGTGGAATGTGTCCTTACGGCTGTCCATATTATTAATCGTCTTCCTACCCTCATTCTTTCCAAAAATACTCCTTTTGAACGATTGTACTTAAAACCACCTTCATTTTCCCACCTTAAAGTCTTCGGTTGTCTTGGTTATGCCACCAACACCCATATCTCTCACAAATTTGCTCCCCGAGCCCACAAATGTGTCTTCCTTGGGTACCCCGTTGGCCAAAAAGCGTACAAACTTTACACCCTTACCACTCATACTTTTTTTACTAGCAGGGATGTTGTTTTTCATGAACATATTTTTCCTTACCATTCTTCTTCATCCTCTACTTCACCTAACCCTACGCCACTACCTATCGTCTCTCCTCACCCTACACCCTCTTTACCTGAGCCTCAAGCTACTACACATTCTACCTCACCCGTGTCTCCTGATACTCCTACCACTTTTTTTTCGCCCGAGATTTCCACCAATTATGACCTACACCCTGATGCATCTTCTCTCGAGCCTGCTGCCTCCCTGCCTCCTGCACCACCTGCACCGATGCTTCACCGCTCTAGCCGCCACTCCTCACCGCCTCCCAGGTTGCGAGATTATGACTACCCCACTCTTAAGCTTACCCAACCCGATCCTCTATCGTCTTCGTTGTCTGGTCCTGCTACTGGTACACGTTATCCACTTGCTAATTATCTCACTTATCATCGTTTCTCACCTGCACAACAATCTTTTCTTGCGACTATCACTCAACAGGTTGAGCCCCAAACTTCTGTCGAGGTTGCTGCTTCCCCTCATTGGCAGGCTGCCATGACTTCTGAACTACAGGCTCTCGAAGCTAATCACACGTGGACTCTCACCTCTCTTCCGGCTGGTAAATCTCCCATTGGTTGTCGATGGCAAGGCAAGTCTTTTACAGCTCTTTTAATTTATGTTGATGATATTCTGATCACTGGAAATGATCCTGATGCCATTAGGGATCTCAAAAATTTTCTCAATAGTCGTTTCAAAATCAAGGACCTTGGTGACTTGAAATACTTTCTAGGCATCGAAGTGTCACGATCAAAGAATGGGATTTTCATTTCTCAATGTAAGTATGCTTTAGAGATTCTGAAGGATGGTGGTCTCTTGGGAGCTCGTCCAATCGAGTTTCCTATGGAGCTGAATCTTAAACTTTCTGACAATGGTGAGTTACTTAAAGATCCAACACAGTATAGGAGGTTGGTGGGTCGATTGATCTATTTAACAATTACTAGGCCGGATATCACATATTCGGTGCATGTGCTAAGTAGGTTTATGCATGAACCTCGCCGTCCACACATGGAAGCAGCTCTCCGAGTTTTGAAATACATAAAGAATACTCCTGGACAAGGTTTATTTTTCTCTTCTAAAAATGACTTGACCTTGAATGCATACTGTGATTCAGATTGGGCAGGATGTTCTATGACACGAAGATCAACCACAGGGTATTGCGTGTTTTTGGGTTCTTCATTGGTTTCTTGGAGATCAAAGAGACAGAAAACCGTATCTTTATCATCAGCTGAGGCCGAGTATAGAGCCATGACTGGAACTTGTTGTGAACTATCATGGCTTCGCTATCTTCTGAAGGACTTGGGATTGTTGCATCCAAAACCGGCCTTATTACATTGTGACAACAAAGCAGCTCTTCATATTGCTGCCAATCCAGTTTTCCATGAGCGGACTAGACATATAGAGATAGATTGCCATTTTGTTCGAGATAAAATACAAGATGGTTCTATTGTTACGCGATTTGTTCCTTCTTCACAACAACTTGCAGATGTGTTCACGAAGGCATTGGGAAAGGAATCTTTCTTGTCCATTATACACAAGTTGGGAGTTCTTGACATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

1283

Amino Acids

143.54

Weight (kDa)

9.07

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 25 - 71 3.7e-20 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 51 - 224 1.4e-11 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 367 - 434 1.2e-07 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 452 - 518 5.1e-12 GAG-pre-integrase domain
rve PF00665 531 - 630 7.4e-13 Integrase core domain
SH3_retrovirus PF25597 704 - 765 6.7e-20 Retroviral polymerase SH3-like domain
RVT_2 PF07727 964 - 1042 2.5e-17 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2515, 2692
AatII GACGTC 1 cut(s) 42
Acc36I ACCTGC 2 cut(s) 2515, 2692
Acc65I GGTACC 1 cut(s) 2184
AccB1I GGYRCC 1 cut(s) 2184
AccBSI CCGCTC 2 cut(s) 2526, 3664
AccII CGCG 2 cut(s) 1803, 3733
AciI CCGC 5 cut(s) 32, 2524, 2533, 2546, 3664
AclI AACGTT 1 cut(s) 1370
AclWI GGATC 7 cut(s) 751, 1603, 1857, 2594, 2921, 2952, 3161
AcoI YGGCCR 2 cut(s) 3, 2194
AcsI RAATTY 5 cut(s) 15, 303, 1820, 2147, 2953
AcuI CTGAAG 3 cut(s) 485, 3098, 3590
AcvI CACGTG 2 cut(s) 686, 2814
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 7 cut(s) 199, 452, 772, 2069, 2186, 2207, 2638
AfiI CCNNNNNNNGG 8 cut(s) 119, 120, 121, 591, 866, 2633, 2763, 3335
AflII CTTAAG 1 cut(s) 2582
AflIII ACRYGT 4 cut(s) 515, 1801, 2639, 2811
AjnI CCWGG 4 cut(s) 137, 1845, 2552, 3328
AjuI GAANNNNNNNTTGG 4 cut(s) 1950, 1982, 3638, 3670
AleI CACNNNNGTG 1 cut(s) 2169
Alw21I GWGCWC 1 cut(s) 3103
Alw26I GTCTC 9 cut(s) 147, 569, 1030, 1225, 1390, 2338, 2403, 3095, 3472
AlwI GGATC 7 cut(s) 751, 1603, 1857, 2594, 2921, 2952, 3161
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 3 cut(s) 2157, 2429, 2474
AoxI GGCC 6 cut(s) 3, 633, 2194, 3221, 3510, 3600
ApeKI GCWGC 9 cut(s) 446, 704, 1052, 2483, 2750, 2771, 3293, 3626, 3641
ApoI RAATTY 5 cut(s) 15, 303, 1820, 2147, 2953
ArsI GACNNNNNNTTYG 2 cut(s) 1222, 1254
AseI ATTAAT 2 cut(s) 545, 2016
Asp718I GGTACC 1 cut(s) 2184
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 5 cut(s) 634, 1628, 1858, 2624, 2983
AsuC2I CCSGG 2 cut(s) 834, 964
AvaI CYCGRG 3 cut(s) 2157, 2429, 2474
AvaII GGWCC 4 cut(s) 1628, 1858, 2624, 2983
BaeGI GKGCMC 1 cut(s) 1281
BalI TGGCCA 2 cut(s) 5, 2196
BanI GGYRCC 1 cut(s) 2184
BanII GRGCYC 3 cut(s) 2164, 2730, 3103
BarI GAAGNNNNNNTAC 2 cut(s) 886, 918
BauI CACGAG 3 cut(s) 144, 336, 1941
BbrPI CACGTG 2 cut(s) 686, 2814
BbsI GAAGAC 7 cut(s) 836, 870, 1067, 2015, 2095, 2167, 2604
Bbv12I GWGCWC 1 cut(s) 3103
BbvCI CCTCAGC 1 cut(s) 3507
BbvI GCAGC 9 cut(s) 433, 716, 1039, 2470, 2737, 2758, 3305, 3628, 3638
BccI CCATC 7 cut(s) 97, 188, 1092, 1151, 2856, 3077, 3710
BceAI ACGGC 2 cut(s) 2016, 3261
BciT130I CCWGG 4 cut(s) 139, 1847, 2554, 3330
BciVI GTATCC 1 cut(s) 837
BclI TGATCA 3 cut(s) 235, 781, 2913
BcnI CCSGG 2 cut(s) 834, 964
BcoDI GTCTC 9 cut(s) 147, 569, 1030, 1225, 1390, 2338, 2403, 3095, 3472
BfaI CTAG 7 cut(s) 182, 1470, 2244, 2529, 3008, 3219, 3669
BfmI CTRYAG 2 cut(s) 577, 2789
BfrI CTTAAG 1 cut(s) 2582
BfuAI ACCTGC 2 cut(s) 2515, 2692
BfuI GTATCC 1 cut(s) 837
BglII AGATCT 1 cut(s) 668
Bme1390I CCNGG 6 cut(s) 139, 834, 964, 1847, 2554, 3330
Bme18I GGWCC 4 cut(s) 1628, 1858, 2624, 2983
BmeT110I CYCGRG 3 cut(s) 2157, 2429, 2474
BmgT120I GGNCC 5 cut(s) 634, 1628, 1858, 2624, 2983
BmiI GGNNCC 3 cut(s) 1629, 1859, 2186
BmrFI CCNGG 6 cut(s) 139, 834, 964, 1847, 2554, 3330
BmrI ACTGGG 1 cut(s) 1236
BmuI ACTGGG 1 cut(s) 1236
BpiI GAAGAC 7 cut(s) 836, 870, 1067, 2015, 2095, 2167, 2604
BpmI CTGGAG 1 cut(s) 1674
Bpu10I CCTNAGC 2 cut(s) 2361, 3507
BpuEI CTTGAG 3 cut(s) 411, 1730, 2352
BpuMI CCSGG 2 cut(s) 834, 964
BsaAI YACGTR 3 cut(s) 686, 1636, 2814
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 3 cut(s) 147, 569, 3095
BsaJI CCNNGG 4 cut(s) 833, 2179, 2552, 2986
BsaXI ACNNNNNCTCC 4 cut(s) 485, 515, 1008, 1038
Bsc4I CCNNNNNNNGG 8 cut(s) 119, 120, 121, 591, 866, 2633, 2763, 3335
Bse118I RCCGGY 1 cut(s) 3598
Bse1I ACTGG 7 cut(s) 489, 1184, 1231, 2638, 2923, 3535, 3650
Bse3DI GCAATG 4 cut(s) 156, 265, 385, 411
BseBI CCWGG 4 cut(s) 139, 1847, 2554, 3330
BseDI CCNNGG 4 cut(s) 833, 2179, 2552, 2986
BseGI GGATG 9 cut(s) 484, 1687, 1740, 2257, 2294, 3088, 3407, 3589, 3831
BseLI CCNNNNNNNGG 8 cut(s) 119, 120, 121, 591, 866, 2633, 2763, 3335
BseMI GCAATG 4 cut(s) 156, 265, 385, 411
BseMII CTCAG 2 cut(s) 2352, 3498
BseNI ACTGG 7 cut(s) 489, 1184, 1231, 2638, 2923, 3535, 3650
BseRI GAGGAG 4 cut(s) 365, 1126, 2328, 2530
BseSI GKGCMC 1 cut(s) 1281
BseXI GCAGC 9 cut(s) 433, 716, 1039, 2470, 2737, 2758, 3305, 3628, 3638
BsgI GTGCAG 4 cut(s) 496, 2484, 2493, 2670
Bsh1236I CGCG 2 cut(s) 1803, 3733
BshFI GGCC 6 cut(s) 5, 635, 2196, 3223, 3512, 3602
BshNI GGYRCC 1 cut(s) 2184
BsiHKAI GWGCWC 1 cut(s) 3103
BsiHKCI CYCGRG 3 cut(s) 2157, 2429, 2474
BsiSI CCGG 5 cut(s) 833, 963, 2834, 3224, 3599
BslFI GGGAC 3 cut(s) 137, 422, 1243
BslI CCNNNNNNNGG 8 cut(s) 119, 120, 121, 591, 866, 2633, 2763, 3335
BsmAI GTCTC 9 cut(s) 147, 569, 1030, 1225, 1390, 2338, 2403, 3095, 3472
BsmBI CGTCTC 2 cut(s) 1390, 2338
BsmFI GGGAC 3 cut(s) 137, 422, 1243
BsmI GAATGC 1 cut(s) 3379
BsnI GGCC 6 cut(s) 5, 635, 2196, 3223, 3512, 3602
Bso31I GGTCTC 3 cut(s) 147, 569, 3095
BsoBI CYCGRG 3 cut(s) 2157, 2429, 2474
Bsp1286I GDGCHC 4 cut(s) 1281, 2164, 2730, 3103
BspACI CCGC 5 cut(s) 32, 2524, 2533, 2546, 3664
BspANI GGCC 6 cut(s) 5, 635, 2196, 3223, 3512, 3602
BspCNI CTCAG 2 cut(s) 2353, 3499
BspFNI CGCG 2 cut(s) 1803, 3733
BspHI TCATGA 3 cut(s) 784, 1002, 2263
BspLI GGNNCC 3 cut(s) 1629, 1859, 2186
BspMI ACCTGC 2 cut(s) 2515, 2692
BspPI GGATC 7 cut(s) 751, 1603, 1857, 2594, 2921, 2952, 3161
BspQI GCTCTTC 2 cut(s) 390, 3636
BspT107I GGYRCC 1 cut(s) 2184
BspTI CTTAAG 1 cut(s) 2582
BspTNI GGTCTC 3 cut(s) 147, 569, 3095
BsrBI CCGCTC 2 cut(s) 2526, 3664
BsrDI GCAATG 4 cut(s) 156, 265, 385, 411
BsrFI RCCGGY 1 cut(s) 3598
BsrI ACTGG 7 cut(s) 489, 1184, 1231, 2638, 2923, 3535, 3650
BssAI RCCGGY 1 cut(s) 3598
BssECI CCNNGG 4 cut(s) 833, 2179, 2552, 2986
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 3 cut(s) 144, 336, 1941
BssT1I CCWWGG 2 cut(s) 2179, 2986
Bst2BI CACGAG 3 cut(s) 144, 336, 1941
Bst2UI CCWGG 4 cut(s) 139, 1847, 2554, 3330
Bst6I CTCTTC 7 cut(s) 390, 651, 936, 1836, 2835, 3355, 3636
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 2582
BstAPI GCANNNNNTGC 1 cut(s) 2507
BstBAI YACGTR 3 cut(s) 686, 1636, 2814
BstC8I GCNNGC 5 cut(s) 1209, 1213, 1916, 2481, 2769
BstDEI CTNAG 4 cut(s) 164, 2361, 3251, 3507
BstENI CCTNNNNNAGG 2 cut(s) 864, 3333
BstF5I GGATG 9 cut(s) 484, 1687, 1740, 2257, 2294, 3088, 3407, 3589, 3831
BstFNI CGCG 2 cut(s) 1803, 3733
BstHHI GCGC 1 cut(s) 526
BstMAI GTCTC 9 cut(s) 147, 569, 1030, 1225, 1390, 2338, 2403, 3095, 3472
BstMWI GCNNNNNNNGC 8 cut(s) 239, 710, 1049, 1800, 2159, 2507, 2532, 2801
BstNI CCWGG 4 cut(s) 139, 1847, 2554, 3330
BstNSI RCATGY 3 cut(s) 233, 519, 3248
BstSCI CCNGG 6 cut(s) 137, 832, 962, 1845, 2552, 3328
BstSFI CTRYAG 2 cut(s) 577, 2789
BstSLI GKGCMC 1 cut(s) 1281
BstSNI TACGTA 1 cut(s) 1636
BstUI CGCG 2 cut(s) 1803, 3733
BstV1I GCAGC 9 cut(s) 433, 716, 1039, 2470, 2737, 2758, 3305, 3628, 3638
BstV2I GAAGAC 7 cut(s) 836, 870, 1067, 2015, 2095, 2167, 2604
BstX2I RGATCY 4 cut(s) 668, 743, 2944, 3166
BstXI CCANNNNNNTGG 2 cut(s) 1853, 1898
BstYI RGATCY 4 cut(s) 668, 743, 2944, 3166
BsuI GTATCC 1 cut(s) 837
BsuRI GGCC 6 cut(s) 5, 635, 2196, 3223, 3512, 3602
BtsCI GGATG 9 cut(s) 484, 1687, 1740, 2257, 2294, 3088, 3407, 3589, 3831
BtsIMutI CAGTG 5 cut(s) 6, 502, 1117, 1224, 2916
BveI ACCTGC 2 cut(s) 2515, 2692
Cac8I GCNNGC 5 cut(s) 1209, 1213, 1916, 2481, 2769
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 3 cut(s) 784, 1002, 2263
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 1 cut(s) 3598
Cfr13I GGNCC 5 cut(s) 634, 1628, 1858, 2624, 2983
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 7 cut(s) 198, 451, 771, 2068, 2185, 2206, 2637
CviQI GTAC 7 cut(s) 198, 451, 771, 2068, 2185, 2206, 2637
DdeI CTNAG 4 cut(s) 164, 2361, 3251, 3507
EaeI YGGCCR 2 cut(s) 3, 2194
Eam1104I CTCTTC 7 cut(s) 390, 651, 936, 1836, 2835, 3355, 3636
EarI CTCTTC 7 cut(s) 390, 651, 936, 1836, 2835, 3355, 3636
Ecl136II GAGCTC 1 cut(s) 3101
Eco105I TACGTA 1 cut(s) 1636
Eco130I CCWWGG 2 cut(s) 2179, 2986
Eco24I GRGCYC 3 cut(s) 2164, 2730, 3103
Eco31I GGTCTC 3 cut(s) 147, 569, 3095
Eco32I GATATC 1 cut(s) 3229
Eco47I GGWCC 4 cut(s) 1628, 1858, 2624, 2983
Eco53kI GAGCTC 1 cut(s) 3101
Eco57I CTGAAG 3 cut(s) 485, 3098, 3590
Eco72I CACGTG 2 cut(s) 686, 2814
Eco88I CYCGRG 3 cut(s) 2157, 2429, 2474
EcoICRI GAGCTC 1 cut(s) 3101
EcoNI CCTNNNNNAGG 2 cut(s) 864, 3333
EcoO109I RGGNCCY 3 cut(s) 1628, 1858, 2983
EcoRII CCWGG 4 cut(s) 137, 1845, 2552, 3328
EcoRV GATATC 1 cut(s) 3229
EcoT14I CCWWGG 2 cut(s) 2179, 2986
EcoT22I ATGCAT 3 cut(s) 2467, 3267, 3379
EcoT38I GRGCYC 3 cut(s) 2164, 2730, 3103
ErhI CCWWGG 2 cut(s) 2179, 2986
Esp3I CGTCTC 2 cut(s) 1390, 2338
FaqI GGGAC 3 cut(s) 137, 422, 1243
FbaI TGATCA 3 cut(s) 235, 781, 2913
FokI GGATG 9 cut(s) 491, 1694, 1727, 2264, 2281, 3095, 3414, 3576, 3818
FriOI GRGCYC 3 cut(s) 2164, 2730, 3103
FspBI CTAG 7 cut(s) 182, 1470, 2244, 2529, 3008, 3219, 3669
GlaI GCGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 1674
HaeIII GGCC 6 cut(s) 5, 635, 2196, 3223, 3512, 3602
HapII CCGG 5 cut(s) 833, 963, 2834, 3224, 3599
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HindIII AAGCTT 1 cut(s) 2585
HpaII CCGG 5 cut(s) 833, 963, 2834, 3224, 3599
Hpy166II GTNNAC 7 cut(s) 1562, 1807, 2639, 2817, 3281, 3545, 3771
Hpy8I GTNNAC 7 cut(s) 1562, 1807, 2639, 2817, 3281, 3545, 3771
Hpy99I CGWCG 3 cut(s) 1098, 1318, 1910
HpyCH4IV ACGT 8 cut(s) 39, 685, 1093, 1345, 1370, 1635, 2641, 2813
HpyF10VI GCNNNNNNNGC 8 cut(s) 239, 710, 1049, 1800, 2159, 2507, 2532, 2801
HpyF3I CTNAG 4 cut(s) 164, 2361, 3251, 3507
HpySE526I ACGT 8 cut(s) 39, 685, 1093, 1345, 1370, 1635, 2641, 2813
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 1 cut(s) 524
KpnI GGTACC 1 cut(s) 2188
Ksp22I TGATCA 3 cut(s) 235, 781, 2913
LguI GCTCTTC 2 cut(s) 390, 3636
LmnI GCTCC 4 cut(s) 521, 2158, 3098, 3124
Lsp1109I GCAGC 9 cut(s) 433, 716, 1039, 2470, 2737, 2758, 3305, 3628, 3638
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 7 cut(s) 182, 1470, 2244, 2529, 3008, 3219, 3669
MaeII ACGT 8 cut(s) 39, 685, 1093, 1345, 1370, 1635, 2641, 2813
MbiI CCGCTC 2 cut(s) 2526, 3664
MflI RGATCY 4 cut(s) 668, 743, 2944, 3166
MhlI GDGCHC 4 cut(s) 1281, 2164, 2730, 3103
MlsI TGGCCA 2 cut(s) 5, 2196
MluI ACGCGT 1 cut(s) 1801
MluNI TGGCCA 2 cut(s) 5, 2196
MlyI GAGTC 5 cut(s) 494, 581, 699, 1665, 2812
MmeI TCCRAC 1 cut(s) 3194
Mox20I TGGCCA 2 cut(s) 5, 2196
Mph1103I ATGCAT 3 cut(s) 2467, 3267, 3379
MscI TGGCCA 2 cut(s) 5, 2196
MslI CAYNNNNRTG 3 cut(s) 2169, 2460, 3017
Msp20I TGGCCA 2 cut(s) 5, 2196
MspA1I CMGCKG 1 cut(s) 3506
MspCI CTTAAG 1 cut(s) 2582
MspI CCGG 5 cut(s) 833, 963, 2834, 3224, 3599
MspR9I CCNGG 6 cut(s) 139, 834, 964, 1847, 2554, 3330
Mva1269I GAATGC 1 cut(s) 3379
MvaI CCWGG 4 cut(s) 139, 1847, 2554, 3330
MvnI CGCG 2 cut(s) 1803, 3733
MwoI GCNNNNNNNGC 8 cut(s) 239, 710, 1049, 1800, 2159, 2507, 2532, 2801
NciI CCSGG 2 cut(s) 834, 964
NlaIV GGNNCC 3 cut(s) 1629, 1859, 2186
NmeAIII GCCGAG 1 cut(s) 3538
NmuCI GTSAC 7 cut(s) 497, 691, 1179, 1922, 2990, 3021, 3614
NsiI ATGCAT 3 cut(s) 2467, 3267, 3379
NspI RCATGY 3 cut(s) 233, 519, 3248
OliI CACNNNNGTG 1 cut(s) 2169
PaeR7I CTCGAG 1 cut(s) 2474
PagI TCATGA 3 cut(s) 784, 1002, 2263
PaqCI CACCTGC 2 cut(s) 2515, 2692
PciI ACATGT 1 cut(s) 515
PciSI GCTCTTC 2 cut(s) 390, 3636
PcsI WCGNNNNNNNCGW 1 cut(s) 1948
PctI GAATGC 1 cut(s) 3379
PfeI GAWTC 7 cut(s) 156, 296, 1375, 3073, 3130, 3386, 3791
PfoI TCCNGGA 2 cut(s) 1845, 3328
PleI GAGTC 5 cut(s) 494, 580, 698, 1664, 2812
PmaCI CACGTG 2 cut(s) 686, 2814
PmlI CACGTG 2 cut(s) 686, 2814
PpsI GAGTC 5 cut(s) 494, 580, 698, 1664, 2812
Ppu21I YACGTR 3 cut(s) 686, 1636, 2814
PpuMI RGGWCCY 3 cut(s) 1628, 1858, 2983
PscI ACATGT 1 cut(s) 515
PshBI ATTAAT 2 cut(s) 545, 2016
Psp124BI GAGCTC 1 cut(s) 3103
Psp1406I AACGTT 1 cut(s) 1370
Psp5II RGGWCCY 3 cut(s) 1628, 1858, 2983
Psp6I CCWGG 4 cut(s) 137, 1845, 2552, 3328
PspCI CACGTG 2 cut(s) 686, 2814
PspGI CCWGG 4 cut(s) 137, 1845, 2552, 3328
PspN4I GGNNCC 3 cut(s) 1629, 1859, 2186
PspPI GGNCC 5 cut(s) 634, 1628, 1858, 2624, 2983
PspPPI RGGWCCY 3 cut(s) 1628, 1858, 2983
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 4 cut(s) 668, 743, 2944, 3166
PvuII CAGCTG 1 cut(s) 3506
RsaI GTAC 7 cut(s) 199, 452, 772, 2069, 2186, 2207, 2638
RsaNI GTAC 7 cut(s) 198, 451, 771, 2068, 2185, 2206, 2637
RseI CAYNNNNRTG 3 cut(s) 2169, 2460, 3017
SacI GAGCTC 1 cut(s) 3103
SapI GCTCTTC 2 cut(s) 390, 3636
Sau96I GGNCC 5 cut(s) 634, 1628, 1858, 2624, 2983
SchI GAGTC 5 cut(s) 494, 581, 699, 1665, 2812
ScrFI CCNGG 6 cut(s) 139, 834, 964, 1847, 2554, 3330
SduI GDGCHC 4 cut(s) 1281, 2164, 2730, 3103
SexAI ACCWGGT 1 cut(s) 137
SfcI CTRYAG 2 cut(s) 577, 2789
Sfr274I CTCGAG 1 cut(s) 2474
SinI GGWCC 4 cut(s) 1628, 1858, 2624, 2983
SlaI CTCGAG 1 cut(s) 2474
SmiMI CAYNNNNRTG 3 cut(s) 2169, 2460, 3017
SmlI CTYRAG 5 cut(s) 426, 1745, 2367, 2474, 2582
SmoI CTYRAG 5 cut(s) 426, 1745, 2367, 2474, 2582
SnaBI TACGTA 1 cut(s) 1636
SsiI CCGC 5 cut(s) 32, 2524, 2533, 2546, 3664
SspMI CTAG 7 cut(s) 182, 1470, 2244, 2529, 3008, 3219, 3669
SstI GAGCTC 1 cut(s) 3103
StyD4I CCNGG 6 cut(s) 137, 832, 962, 1845, 2552, 3328
StyI CCWWGG 2 cut(s) 2179, 2986
TaiI ACGT 8 cut(s) 42, 688, 1096, 1348, 1373, 1638, 2644, 2816
TatI WGTACW 2 cut(s) 770, 2067
TauI GCSGC 2 cut(s) 35, 2535
TfiI GAWTC 7 cut(s) 156, 296, 1375, 3073, 3130, 3386, 3791
TscAI CASTG 5 cut(s) 13, 502, 1117, 1231, 2923
TseFI GTSAC 7 cut(s) 497, 691, 1179, 1922, 2990, 3021, 3614
TseI GCWGC 9 cut(s) 446, 704, 1052, 2483, 2750, 2771, 3293, 3626, 3641
Tsp45I GTSAC 7 cut(s) 497, 691, 1179, 1922, 2990, 3021, 3614
TspGWI ACGGA 1 cut(s) 1018
TspRI CASTG 5 cut(s) 13, 502, 1117, 1231, 2923
Vha464I CTTAAG 1 cut(s) 2582
VpaK11BI GGWCC 4 cut(s) 1628, 1858, 2624, 2983
VspI ATTAAT 2 cut(s) 545, 2016
XagI CCTNNNNNAGG 2 cut(s) 864, 3333
XapI RAATTY 5 cut(s) 15, 303, 1820, 2147, 2953
XceI RCATGY 3 cut(s) 233, 519, 3248
XhoI CTCGAG 1 cut(s) 2474
XspI CTAG 7 cut(s) 182, 1470, 2244, 2529, 3008, 3219, 3669
ZraI GACGTC 1 cut(s) 40
Zsp2I ATGCAT 3 cut(s) 2467, 3267, 3379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.