RchiOBHm_Chr4g0404531

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000071
Physical Location & Seq
Reverse (-)
24266313 .. 24269802
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37607

Sequence Viewer

Length: 3450 bp
ATGAAGGGTAAGGCTACTTTAAATAATACATGGATAATTGATACAGGTGCATCTGATCATATGACCAATGACCCTAGTCTTGTGAAAAACCTTAGGCGTTCCTCTCAAAATATTGTCTCTACTGCTGATGGTACTCCAACTCCGGTCACCGGAGAAGGTTCTATTGTTGTATCTGATACCTTAACCCTTGAATCTGTCCTAGTTGTTCCCTCACTAGCTTATAATCTCCTATCTGTTGGTCAGATTATTTTAGCACTTGCATGTATTGTGACCTTCTATCCATCTTTCTGTGTGTTTCAAGACATTCTGACTCGGCGGATTCTTGGTTATGGTGTTAGAAGGGGAAAATTATACTACCTGGATCTGACAGAGACTGGAGAAAACCATAAGCATCTTTTGGGGCAAGCTAATCAAATTAATGGGGTAGAGAATGCAAAGGAAGCAGTATGGTTATGGCATCGCCGTTTAGGTCATCTATCTTTTAGTTATCTTAAGAAGCTGCAACCTCATTTGTTTTCGGTTGTCAGTGATTTGGATTTCCATTGTGACATTTGTGAACTGGCCAAGAGCCACCGTATTTCATATTCACCAAGTCTTCATAAAAGTCCTGTTCCTTTTATGAAAATTCACTCTGATGTCTGGGGTCCTGCAAAGATTCCTTCTCTTTCTGGAGCTCGGTATTTTGTAACGTTTATTGATGATTGCACTCGCATGACATGGGTGTCATTTCTGAAGAATAAGAGTGATGTATTTGGAATGTTTATCGAATTTCACAAAATGGTGGCAACTCAGTATTTACAATCCATCCGAGTGTTTCAGTCTGACAATGGTGGAGAGTTTGTGAATGGCCCTATGATTGAGTTTTGCCGGTCACATGGAATTCGTCATCAAACCTCCAATTCTTATACTCCTCAACAGAATGGGTTAGCAGAACGGAAGAACAGGCAGTTGATGGAAGTTGTTCGTGCTTCCTTATTTGGCATGAATGTACCTCGGTCCTATTGGGGAGAAGCAGTAAAATCTGCAGCATATCTTATCAACCGTACTCCTTCACGGGTGATTGAGTTTCAGAATCCTCATCAGAAGCTTCATACATTTTTGACCATCCCTTCTATGCCTAATTTGGAGCCCCGGGTGTTTGGGTGCACAGCCTATGTTCATATTCCCAAGCCTCAACGCAACAAGCTTGATCCCCGTGCCCGTAAGTGTATCTTTGTTGGTTATGCTGACTTCCAGAAGGGTTATCGATGTTATGATCCCCTTACTGGCACTTTACATGTCTCTCTTGATGTTGCTTTTCGTGAATCCGAGCCTTATTACTCAGGGGGAGCTTCTCAGTCTTCCCTTCAGGGGGAGAGAGGTTGTGAAGGGAATCCTCGTTCTATTATTGATTTTGATGTCTTTAAAGACTTGGAAAATTTGGAGGATACATTTGAGGGTAGAAAAAATTTGGAAACAGAAAATGCAGTTGCCGAACAGAGCATTGTGAATTCCGAAATAGACAATGCGACTGCCGAACGAAGTGTTGCGAATTCCGAAACAGAAAATGCGACTGCCGAACAGAGTGTTGTGAATTCCGAAACAGAAAATGTAACTGCCGAACAGAGTGTTGTGAATTCCGAGACAGAAGAGACGACTTTTCTGGATAGTTTGAATCAAAATCAAGATGTATCTGAAGCTCACACACAAGATATTCCCCCTTCTACATCACCAACTGAAGATCCCGGTCAGAATGATCCGCCTCAGGTACCCCTAAACTTTAATGAATCTTCTGGGTTAGAAAGTGTCGAACCTAGGAAATCACAAAGGGTTGCCAAGGGAATTCCTAAGAAACAATATGAACCAGATATCAAAGCCAAAGCTAAATACCCTATAGCTAATTTTATGTCTAACCATAGGATTTCTGGGTCACATGCACTTGTTATCGATCAATTATCTACTGTATCTATTCCTAGTAACGTGCAGGATGCATTAACGAATCCAAAATGGACAAAGGCGATGAATGAAGAATTGGAAGCTCTTCAAAAGAATGCAACATGGGAACTAGTACCTATGCCGGTTGGAAAGAAGACTGTAGGGTGTCGTTGGGTATTTACTGTGAAGCTTAATGCAGATGGAACTATTAATAGATACAAGGCGAGGTTGGTTGCCAAAGGATACACACAACGTTATGGGATTGATTATGAGGAGACTTTTGCACCTGTGGCAAAGATCAATACTGTTCGGATTCTAATCTCACTTGCAGCAAACAAAGATTGGCCCTTACACCAGTTTGATGTGAAGAATGCGTTTCTTAATGGGAATTTGGAAGAAGAAGTGTACATGGATGTGCCCCCAGGTGTTAAGAATTACCCAAGTGAAGTTGGCAAGGTGTGTAAATTGAAGAAGTCTTTGTATGGCCTGAAGCAATCTCCAAGAGCCTGGTTTGGGAGATTTTCAAAGTCCATGAGAGCCTTTGGGTACAGACAGAGCAATTCTGACCATACCTTGTTTATCAAGCGCAAGAATGGTAAGATTACAGCTCTTATTGTGTATGTTGATGACATGATTGTTACAGGGGATGATCCAAAAGAGATGAATGAGTTACAAAAGTATCTGTCAAAGGAGTTTGAAATGAAGGATCTGGGGCAACTGAAGTATTTTCTGGGTATTGAAGTTGCGAGGTCTAAGAAGGGAATTTCACTGTCACAGAGGAAGTATGTTCTTGACTTACTTACTGAAACGGGGATGCTGGACTGCAGTCCAATTGAGACACCCATTGAGATGAATCACAGACTTGCCATTTATCCTGATCAAATTCCAACTGATAAAGGAAGGTATCAACGTCTTGTAGGAAGGTTGATTTATCTTTCACATACTAGACCTGATATTGCTTATGCTAACATATGGATGCAGTTTTTTCGGATTTTGAGGTATTTGAAGATGGCGCCAGGTAAAGGATTACTGTTTGAGAAAAAGGATGAATTGGAAGTTGGGGGATACACATATGCAGATTGGGCTGGTGATAAAACTGACAGGCGTTCTACATCTGGGTACTTCACTTTTGTAGGAGGGAACCTTGTCACTTGGCGTAGCAAGAAACAGAAAGTTGTGGCCAGATCAAGTGCAGAAGCTGAATTTCGAGGTATGGCACATGGAGTCTGTGAAATGTTATGGATTCGTAATGTCCTGAAAGATCTAGGTTACAAGCTTAGACAACCTATGGATTTGCATTGTGATAATAAAGCTGCAATTGAGATTGCACATAATCCAGTTCAGCATGATAGGACAAAGCATGTGGAGGTTGACCGTCATTTTATTAAAGAAAATCTTGACAGAAAGGTTATTCGTTTTCCATTTGTAAACTCAAAAGAACAGTTAGCTGATGTTCTTACTAAAGGAGTGTGCAGGAAGATATTTGACAGCTCAGTTGACAAGTTGGGCATGATAGATATCTATGCACCAACTTGA

Protein Analysis

1149

Amino Acids

129.99

Weight (kDa)

8.65

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 11 - 82 2.8e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 117 - 190 2e-15 GAG-pre-integrase domain
rve PF00665 208 - 304 3.8e-11 Integrase core domain
SH3_retrovirus PF25597 382 - 439 2.7e-25 Retroviral polymerase SH3-like domain
RVT_2 PF07727 677 - 921 3.1e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AasI GACNNNNNNGTC 1 cut(s) 721
Acc65I GGTACC 1 cut(s) 1747
AccB1I GGYRCC 2 cut(s) 1747, 2926
AciI CCGC 2 cut(s) 316, 1739
AclI AACGTT 2 cut(s) 689, 2167
AclWI GGATC 7 cut(s) 369, 1184, 1250, 1715, 1730, 2558, 2628
AcoI YGGCCR 2 cut(s) 561, 3093
AcuI CTGAAG 6 cut(s) 752, 1331, 1695, 1737, 2423, 2654
AcyI GRCGYC 1 cut(s) 2927
AfaI GTAC 8 cut(s) 133, 990, 1045, 1749, 2049, 2321, 2462, 3035
AfiI CCNNNNNNNGG 6 cut(s) 149, 1265, 1350, 1351, 2427, 2936
AflII CTTAAG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 1276
AgsI TTSAA 9 cut(s) 191, 299, 1654, 2024, 2383, 2439, 2612, 2654, 2920
AhlI ACTAGT 1 cut(s) 2044
AjnI CCWGG 4 cut(s) 357, 2335, 2420, 2929
AjuI GAANNNNNNNTTGG 4 cut(s) 1994, 2026, 2955, 2987
AloI GAACNNNNNNTCC 2 cut(s) 1363, 1395
Alw21I GWGCWC 2 cut(s) 676, 1148
Alw26I GTCTC 7 cut(s) 121, 365, 1285, 1616, 1625, 2183, 2744
Alw44I GTGCAC 1 cut(s) 1144
AlwI GGATC 7 cut(s) 369, 1184, 1250, 1715, 1730, 2558, 2628
AlwNI CAGNNNCTG 2 cut(s) 374, 3113
Ama87I CYCGRG 1 cut(s) 1131
AoxI GGCC 5 cut(s) 561, 847, 2258, 2398, 3093
ApaLI GTGCAC 1 cut(s) 1144
ApeKI GCWGC 4 cut(s) 499, 1025, 2243, 3227
AseI ATTAAT 2 cut(s) 417, 2124
Asp700I GAANNNNTTC 2 cut(s) 960, 2019
Asp718I GGTACC 1 cut(s) 1747
AspA2I CCTAGG 1 cut(s) 1793
AspLEI GCGC 2 cut(s) 2502, 2929
AspS9I GGNCC 4 cut(s) 644, 848, 996, 2259
AsuC2I CCSGG 3 cut(s) 1132, 1133, 1725
AsuHPI GGTGA 5 cut(s) 139, 579, 1069, 1701, 3014
AvaI CYCGRG 1 cut(s) 1131
AvaII GGWCC 2 cut(s) 644, 996
AvrII CCTAGG 1 cut(s) 1793
AxyI CCTNAGG 2 cut(s) 92, 1743
BaeGI GKGCMC 3 cut(s) 1148, 1201, 2334
BaeI ACNNNNGTAYC 2 cut(s) 2576, 2609
BalI TGGCCA 2 cut(s) 563, 3095
BanI GGYRCC 2 cut(s) 1747, 2926
BanII GRGCYC 2 cut(s) 676, 1131
BbsI GAAGAC 3 cut(s) 587, 1332, 2075
Bbv12I GWGCWC 2 cut(s) 676, 1148
BbvI GCAGC 4 cut(s) 486, 1037, 2255, 3214
BccI CCATC 7 cut(s) 122, 289, 812, 946, 1112, 2108, 2917
BceAI ACGGC 1 cut(s) 447
BcgI CGANNNNNNTGC 2 cut(s) 2882, 2916
BciT130I CCWGG 4 cut(s) 359, 2337, 2422, 2931
BciVI GTATCC 3 cut(s) 1420, 2150, 2972
BclI TGATCA 2 cut(s) 55, 2791
BcnI CCSGG 3 cut(s) 1132, 1133, 1725
BcoDI GTCTC 7 cut(s) 121, 365, 1285, 1616, 1625, 2183, 2744
BcuI ACTAGT 1 cut(s) 2044
BfaI CTAG 8 cut(s) 75, 200, 215, 1794, 1953, 2045, 2859, 3179
BfmI CTRYAG 4 cut(s) 1023, 1872, 2073, 2737
BfoI RGCGCY 1 cut(s) 2930
BfrI CTTAAG 1 cut(s) 491
BfuI GTATCC 3 cut(s) 1420, 2150, 2972
BglII AGATCT 1 cut(s) 3175
BisI GCNGC 4 cut(s) 500, 1026, 2244, 3228
BlnI CCTAGG 1 cut(s) 1793
BlsI GCNGC 4 cut(s) 501, 1027, 2245, 3229
Bme1390I CCNGG 7 cut(s) 359, 1132, 1133, 1725, 2337, 2422, 2931
Bme18I GGWCC 2 cut(s) 644, 996
BmeT110I CYCGRG 1 cut(s) 1131
BmgT120I GGNCC 4 cut(s) 644, 848, 996, 2259
BmiI GGNNCC 5 cut(s) 645, 1128, 1749, 2928, 3056
BmrFI CCNGG 7 cut(s) 359, 1132, 1133, 1725, 2337, 2422, 2931
BmsI GCATC 6 cut(s) 59, 400, 466, 1957, 2718, 2880
BoxI GACNNNNGTC 2 cut(s) 75, 2739
BpiI GAAGAC 3 cut(s) 587, 1332, 2075
BpmI CTGGAG 2 cut(s) 396, 690
BpuMI CCSGG 3 cut(s) 1132, 1133, 1725
Bsa29I ATCGAT 2 cut(s) 1246, 1926
BsaBI GATNNNNATC 2 cut(s) 2231, 3431
BsaHI GRCGYC 1 cut(s) 2927
BsaJI CCNNGG 6 cut(s) 992, 1130, 1131, 1793, 1815, 2335
BsaWI WCCGGW 2 cut(s) 142, 149
BsaXI ACNNNNNCTCC 6 cut(s) 124, 154, 825, 855, 999, 1029
Bsc4I CCNNNNNNNGG 6 cut(s) 149, 1265, 1350, 1351, 2427, 2936
Bse118I RCCGGY 2 cut(s) 867, 2056
Bse1I ACTGG 5 cut(s) 379, 564, 1270, 2269, 3251
Bse21I CCTNAGG 2 cut(s) 92, 1743
Bse8I GATNNNNATC 2 cut(s) 2231, 3431
BseBI CCWGG 4 cut(s) 359, 2337, 2422, 2931
BseCI ATCGAT 2 cut(s) 1246, 1926
BseDI CCNNGG 6 cut(s) 992, 1130, 1131, 1793, 1815, 2335
BseGI GGATG 8 cut(s) 804, 1104, 1972, 2332, 2566, 2733, 2895, 2965
BseJI GATNNNNATC 2 cut(s) 2231, 3431
BseLI CCNNNNNNNGG 6 cut(s) 149, 1265, 1350, 1351, 2427, 2936
BseMII CTCAG 5 cut(s) 803, 1335, 1349, 1757, 3420
BseNI ACTGG 5 cut(s) 379, 564, 1270, 2269, 3251
BseRI GAGGAG 2 cut(s) 900, 2201
BseSI GKGCMC 3 cut(s) 1148, 1201, 2334
BseXI GCAGC 4 cut(s) 486, 1037, 2255, 3214
BsgI GTGCAG 3 cut(s) 1982, 3126, 3406
BshFI GGCC 5 cut(s) 563, 849, 2260, 2400, 3095
BshNI GGYRCC 2 cut(s) 1747, 2926
BshVI ATCGAT 2 cut(s) 1246, 1926
BsiHKAI GWGCWC 2 cut(s) 676, 1148
BsiHKCI CYCGRG 1 cut(s) 1131
BsiSI CCGG 6 cut(s) 143, 150, 868, 1132, 1725, 2057
BslI CCNNNNNNNGG 6 cut(s) 149, 1265, 1350, 1351, 2427, 2936
BsmAI GTCTC 7 cut(s) 121, 365, 1285, 1616, 1625, 2183, 2744
BsmBI CGTCTC 1 cut(s) 1625
BsmI GAATGC 3 cut(s) 436, 2035, 2290
BsnI GGCC 5 cut(s) 563, 849, 2260, 2400, 3095
BsoBI CYCGRG 1 cut(s) 1131
Bsp1286I GDGCHC 5 cut(s) 676, 1131, 1148, 1201, 2334
Bsp1407I TGTACA 1 cut(s) 2319
BspACI CCGC 2 cut(s) 316, 1739
BspANI GGCC 5 cut(s) 563, 849, 2260, 2400, 3095
BspCNI CTCAG 5 cut(s) 802, 1334, 1348, 1756, 3419
BspDI ATCGAT 2 cut(s) 1246, 1926
BspLI GGNNCC 5 cut(s) 645, 1128, 1749, 2928, 3056
BspMAI CTGCAG 2 cut(s) 1027, 2741
BspPI GGATC 7 cut(s) 369, 1184, 1250, 1715, 1730, 2558, 2628
BspQI GCTCTTC 1 cut(s) 2025
BspT107I GGYRCC 2 cut(s) 1747, 2926
BspTI CTTAAG 1 cut(s) 491
BsrFI RCCGGY 2 cut(s) 867, 2056
BsrGI TGTACA 1 cut(s) 2319
BsrI ACTGG 5 cut(s) 379, 564, 1270, 2269, 3251
BssAI RCCGGY 2 cut(s) 867, 2056
BssECI CCNNGG 6 cut(s) 992, 1130, 1131, 1793, 1815, 2335
BssNI GRCGYC 1 cut(s) 2927
BssT1I CCWWGG 2 cut(s) 1793, 1815
Bst2UI CCWGG 4 cut(s) 359, 2337, 2422, 2931
Bst6I CTCTTC 2 cut(s) 1623, 2025
BstACI GRCGYC 1 cut(s) 2927
BstAFI CTTAAG 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 2319
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 9 cut(s) 92, 789, 1321, 1335, 1743, 1827, 2667, 3191, 3406
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 8 cut(s) 804, 1104, 1972, 2332, 2566, 2733, 2895, 2965
BstH2I RGCGCY 1 cut(s) 2930
BstHHI GCGC 2 cut(s) 2502, 2929
BstMAI GTCTC 7 cut(s) 121, 365, 1285, 1616, 1625, 2183, 2744
BstMWI GCNNNNNNNGC 3 cut(s) 440, 2204, 2996
BstNI CCWGG 4 cut(s) 359, 2337, 2422, 2931
BstNSI RCATGY 4 cut(s) 264, 1280, 1916, 3278
BstPAI GACNNNNGTC 2 cut(s) 75, 2739
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 7 cut(s) 357, 1130, 1131, 1723, 2335, 2420, 2929
BstSFI CTRYAG 4 cut(s) 1023, 1872, 2073, 2737
BstSLI GKGCMC 3 cut(s) 1148, 1201, 2334
BstV1I GCAGC 4 cut(s) 486, 1037, 2255, 3214
BstV2I GAAGAC 3 cut(s) 587, 1332, 2075
BstX2I RGATCY 4 cut(s) 361, 1720, 2620, 3175
BstXI CCANNNNNNTGG 1 cut(s) 2421
BstYI RGATCY 4 cut(s) 361, 1720, 2620, 3175
Bsu15I ATCGAT 2 cut(s) 1246, 1926
Bsu36I CCTNAGG 2 cut(s) 92, 1743
BsuI GTATCC 3 cut(s) 1420, 2150, 2972
BsuRI GGCC 5 cut(s) 563, 849, 2260, 2400, 3095
BsuTUI ATCGAT 2 cut(s) 1246, 1926
BtgZI GCGATG 2 cut(s) 443, 2012
BtsCI GGATG 8 cut(s) 804, 1104, 1972, 2332, 2566, 2733, 2895, 2965
BtsIMutI CAGTG 2 cut(s) 532, 2681
Cac8I GCNNGC 1 cut(s) 405
CaiI CAGNNNCTG 2 cut(s) 374, 3113
CfoI GCGC 2 cut(s) 2502, 2929
Cfr10I RCCGGY 2 cut(s) 867, 2056
Cfr13I GGNCC 4 cut(s) 644, 848, 996, 2259
Cfr9I CCCGGG 1 cut(s) 1131
ClaI ATCGAT 2 cut(s) 1246, 1926
Csp6I GTAC 8 cut(s) 132, 989, 1044, 1748, 2048, 2320, 2461, 3034
CspCI CAANNNNNGTGG 2 cut(s) 3258, 3293
CviQI GTAC 8 cut(s) 132, 989, 1044, 1748, 2048, 2320, 2461, 3034
DdeI CTNAG 9 cut(s) 92, 789, 1321, 1335, 1743, 1827, 2667, 3191, 3406
DinI GGCGCC 1 cut(s) 2928
DraI TTTAAA 2 cut(s) 21, 1405
DrdI GACNNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 1 cut(s) 721
EaeI YGGCCR 2 cut(s) 561, 3093
Eam1104I CTCTTC 2 cut(s) 1623, 2025
EarI CTCTTC 2 cut(s) 1623, 2025
EciI GGCGGA 2 cut(s) 331, 1728
Ecl136II GAGCTC 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 1793, 1815
Eco24I GRGCYC 2 cut(s) 676, 1131
Eco32I GATATC 2 cut(s) 1849, 3433
Eco47I GGWCC 2 cut(s) 644, 996
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 6 cut(s) 752, 1331, 1695, 1737, 2423, 2654
Eco81I CCTNAGG 2 cut(s) 92, 1743
Eco88I CYCGRG 1 cut(s) 1131
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 674
EcoO109I RGGNCCY 1 cut(s) 644
EcoO65I GGTNACC 1 cut(s) 145
EcoRI GAATTC 6 cut(s) 879, 1489, 1531, 1573, 1615, 1821
EcoRII CCWGG 4 cut(s) 357, 2335, 2420, 2929
EcoRV GATATC 2 cut(s) 1849, 3433
EcoT14I CCWWGG 2 cut(s) 1793, 1815
EcoT22I ATGCAT 1 cut(s) 1972
EcoT38I GRGCYC 2 cut(s) 676, 1131
EgeI GGCGCC 1 cut(s) 2928
EheI GGCGCC 1 cut(s) 2928
ErhI CCWWGG 2 cut(s) 1793, 1815
Esp3I CGTCTC 1 cut(s) 1625
FalI AAGNNNNNCTT 4 cut(s) 1196, 1228, 3294, 3326
FauNDI CATATG 3 cut(s) 60, 2885, 2986
FbaI TGATCA 2 cut(s) 55, 2791
Fnu4HI GCNGC 4 cut(s) 500, 1026, 2244, 3228
FokI GGATG 8 cut(s) 791, 1091, 1979, 2339, 2573, 2740, 2902, 2972
FriOI GRGCYC 2 cut(s) 676, 1131
Fsp4HI GCNGC 4 cut(s) 500, 1026, 2244, 3228
FspBI CTAG 8 cut(s) 75, 200, 215, 1794, 1953, 2045, 2859, 3179
GlaI GCGC 2 cut(s) 2501, 2928
GluI GCNGC 4 cut(s) 500, 1026, 2244, 3228
GsuI CTGGAG 2 cut(s) 396, 690
HaeII RGCGCY 1 cut(s) 2930
HaeIII GGCC 5 cut(s) 563, 849, 2260, 2400, 3095
HapII CCGG 6 cut(s) 143, 150, 868, 1132, 1725, 2057
HhaI GCGC 2 cut(s) 2502, 2929
Hin1I GRCGYC 1 cut(s) 2927
Hin6I GCGC 2 cut(s) 2500, 2927
HinP1I GCGC 2 cut(s) 2500, 2927
HincII GTYRAC 2 cut(s) 3286, 3412
HindII GTYRAC 2 cut(s) 3286, 3412
HindIII AAGCTT 4 cut(s) 1085, 1184, 2102, 3188
HpaII CCGG 6 cut(s) 143, 150, 868, 1132, 1725, 2057
HphI GGTGA 5 cut(s) 139, 579, 1069, 1701, 3014
Hpy166II GTNNAC 6 cut(s) 557, 1146, 2320, 3286, 3343, 3412
Hpy8I GTNNAC 6 cut(s) 557, 1146, 2320, 3286, 3343, 3412
HpyCH4IV ACGT 4 cut(s) 689, 1959, 2167, 2824
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 2204, 2996
HpyF3I CTNAG 9 cut(s) 92, 789, 1321, 1335, 1743, 1827, 2667, 3191, 3406
HpySE526I ACGT 4 cut(s) 689, 1959, 2167, 2824
Hsp92I GRCGYC 1 cut(s) 2927
HspAI GCGC 2 cut(s) 2500, 2927
KasI GGCGCC 1 cut(s) 2926
KpnI GGTACC 1 cut(s) 1751
Ksp22I TGATCA 2 cut(s) 55, 2791
LguI GCTCTTC 1 cut(s) 2025
LmnI GCTCC 3 cut(s) 671, 1126, 1328
Lsp1109I GCAGC 4 cut(s) 486, 1037, 2255, 3214
LweI GCATC 6 cut(s) 59, 400, 466, 1957, 2718, 2880
MaeI CTAG 8 cut(s) 75, 200, 215, 1794, 1953, 2045, 2859, 3179
MaeII ACGT 4 cut(s) 689, 1959, 2167, 2824
MfeI CAATTG 2 cut(s) 2745, 3231
MflI RGATCY 4 cut(s) 361, 1720, 2620, 3175
MhlI GDGCHC 5 cut(s) 676, 1131, 1148, 1201, 2334
MlsI TGGCCA 2 cut(s) 563, 3095
MluNI TGGCCA 2 cut(s) 563, 3095
Mly113I GGCGCC 1 cut(s) 2927
MlyI GAGTC 2 cut(s) 304, 3147
MmeI TCCRAC 3 cut(s) 161, 2041, 2825
Mox20I TGGCCA 2 cut(s) 563, 3095
Mph1103I ATGCAT 1 cut(s) 1972
MroXI GAANNNNTTC 2 cut(s) 960, 2019
MscI TGGCCA 2 cut(s) 563, 3095
MslI CAYNNNNRTG 7 cut(s) 259, 633, 710, 809, 2327, 2762, 2888
Msp20I TGGCCA 2 cut(s) 563, 3095
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 6 cut(s) 143, 150, 868, 1132, 1725, 2057
MspR9I CCNGG 7 cut(s) 359, 1132, 1133, 1725, 2337, 2422, 2931
MunI CAATTG 2 cut(s) 2745, 3231
Mva1269I GAATGC 3 cut(s) 436, 2035, 2290
MvaI CCWGG 4 cut(s) 359, 2337, 2422, 2931
MwoI GCNNNNNNNGC 3 cut(s) 440, 2204, 2996
NarI GGCGCC 1 cut(s) 2927
NciI CCSGG 3 cut(s) 1132, 1133, 1725
NdeI CATATG 3 cut(s) 60, 2885, 2986
NlaIV GGNNCC 5 cut(s) 645, 1128, 1749, 2928, 3056
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 7 cut(s) 145, 268, 545, 870, 1908, 2685, 3061
NsiI ATGCAT 1 cut(s) 1972
NspI RCATGY 4 cut(s) 264, 1280, 1916, 3278
PciI ACATGT 1 cut(s) 1276
PciSI GCTCTTC 1 cut(s) 2025
PctI GAATGC 3 cut(s) 436, 2035, 2290
PdmI GAANNNNTTC 2 cut(s) 960, 2019
PkrI GCNGC 4 cut(s) 501, 1027, 2245, 3229
PleI GAGTC 2 cut(s) 304, 3146
PluTI GGCGCC 1 cut(s) 2930
PpsI GAGTC 2 cut(s) 304, 3146
PpuMI RGGWCCY 1 cut(s) 644
PscI ACATGT 1 cut(s) 1276
PshAI GACNNNNGTC 2 cut(s) 75, 2739
PshBI ATTAAT 2 cut(s) 417, 2124
PsiI TTATAA 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 676
Psp1406I AACGTT 2 cut(s) 689, 2167
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 4 cut(s) 357, 2335, 2420, 2929
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 4 cut(s) 357, 2335, 2420, 2929
PspN4I GGNNCC 5 cut(s) 645, 1128, 1749, 2928, 3056
PspPI GGNCC 4 cut(s) 644, 848, 996, 2259
PspPPI RGGWCCY 1 cut(s) 644
PstI CTGCAG 2 cut(s) 1027, 2741
PstNI CAGNNNCTG 2 cut(s) 374, 3113
PsuI RGATCY 4 cut(s) 361, 1720, 2620, 3175
RsaI GTAC 8 cut(s) 133, 990, 1045, 1749, 2049, 2321, 2462, 3035
RsaNI GTAC 8 cut(s) 132, 989, 1044, 1748, 2048, 2320, 2461, 3034
RseI CAYNNNNRTG 7 cut(s) 259, 633, 710, 809, 2327, 2762, 2888
SacI GAGCTC 1 cut(s) 676
SapI GCTCTTC 1 cut(s) 2025
SatI GCNGC 4 cut(s) 500, 1026, 2244, 3228
Sau96I GGNCC 4 cut(s) 644, 848, 996, 2259
SchI GAGTC 2 cut(s) 304, 3147
ScrFI CCNGG 7 cut(s) 359, 1132, 1133, 1725, 2337, 2422, 2931
SduI GDGCHC 5 cut(s) 676, 1131, 1148, 1201, 2334
SfaNI GCATC 6 cut(s) 59, 400, 466, 1957, 2718, 2880
SfcI CTRYAG 4 cut(s) 1023, 1872, 2073, 2737
SfoI GGCGCC 1 cut(s) 2928
SinI GGWCC 2 cut(s) 644, 996
SmaI CCCGGG 1 cut(s) 1133
SmiMI CAYNNNNRTG 7 cut(s) 259, 633, 710, 809, 2327, 2762, 2888
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
SpeI ACTAGT 1 cut(s) 2044
SsiI CCGC 2 cut(s) 316, 1739
SspDI GGCGCC 1 cut(s) 2926
SspI AATATT 1 cut(s) 112
SspMI CTAG 8 cut(s) 75, 200, 215, 1794, 1953, 2045, 2859, 3179
SstI GAGCTC 1 cut(s) 676
StyD4I CCNGG 7 cut(s) 357, 1130, 1131, 1723, 2335, 2420, 2929
StyI CCWWGG 2 cut(s) 1793, 1815
TaiI ACGT 4 cut(s) 692, 1962, 2170, 2827
TaqI TCGA 5 cut(s) 765, 1246, 1788, 1926, 3121
TaqII GACCGA 1 cut(s) 984
TatI WGTACW 1 cut(s) 2319
TscAI CASTG 2 cut(s) 532, 2688
TseFI GTSAC 7 cut(s) 145, 268, 545, 870, 1908, 2685, 3061
TseI GCWGC 4 cut(s) 499, 1025, 2243, 3227
Tsp45I GTSAC 7 cut(s) 145, 268, 545, 870, 1908, 2685, 3061
TspGWI ACGGA 1 cut(s) 949
TspMI CCCGGG 1 cut(s) 1131
TspRI CASTG 2 cut(s) 532, 2688
Vha464I CTTAAG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 1144
VpaK11BI GGWCC 2 cut(s) 644, 996
VspI ATTAAT 2 cut(s) 417, 2124
XceI RCATGY 4 cut(s) 264, 1280, 1916, 3278
XcmI CCANNNNNNNNNTGG 1 cut(s) 1901
XmaI CCCGGG 1 cut(s) 1131
XmaJI CCTAGG 1 cut(s) 1793
XmnI GAANNNNTTC 2 cut(s) 960, 2019
XspI CTAG 8 cut(s) 75, 200, 215, 1794, 1953, 2045, 2859, 3179
Zsp2I ATGCAT 1 cut(s) 1972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.