beta-galactosidase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Pol_BBD | PF22936 | 120 - 193 | 2.5e-19 | Pol polyprotein, beta-barrel domain |
| gag_pre-integrs | PF13976 | 242 - 297 | 2.6e-13 | GAG-pre-integrase domain |
| rve | PF00665 | 313 - 412 | 7.8e-09 | Integrase core domain |
| SH3_retrovirus | PF25597 | 490 - 551 | 3.5e-24 | Retroviral polymerase SH3-like domain |
| RVT_2 | PF07727 | 741 - 984 | 4.2e-74 | Reverse transcriptase (RNA-dependent DNA polymerase) |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1361, 3530 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1868 |
| Acc36I | ACCTGC | 1 cut(s) | 2907 |
| AccB1I | GGYRCC | 1 cut(s) | 1828 |
| AccI | GTMKAC | 3 cut(s) | 2003, 2511, 2990 |
| AccII | CGCG | 1 cut(s) | 2607 |
| AciI | CCGC | 4 cut(s) | 158, 727, 732, 2638 |
| AclWI | GGATC | 5 cut(s) | 493, 636, 2165, 2178, 2317 |
| AcoI | YGGCCR | 2 cut(s) | 882, 2450 |
| AcsI | RAATTY | 8 cut(s) | 7, 745, 765, 1395, 1675, 3132, 3394, 3612 |
| AcuI | CTGAAG | 4 cut(s) | 1370, 1649, 1878, 3600 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 476, 2252, 2983, 3164 |
| AflIII | ACRYGT | 1 cut(s) | 872 |
| AjiI | CACGTC | 1 cut(s) | 1727 |
| AjnI | CCWGG | 8 cut(s) | 638, 712, 1824, 1909, 2527, 2548, 2849, 3157 |
| Alw21I | GWGCWC | 3 cut(s) | 721, 1309, 3004 |
| Alw26I | GTCTC | 7 cut(s) | 337, 994, 1429, 2083, 2375, 2594, 2903 |
| AlwI | GGATC | 5 cut(s) | 493, 636, 2165, 2178, 2317 |
| Ama87I | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| AoxI | GGCC | 3 cut(s) | 882, 1473, 2450 |
| ApeKI | GCWGC | 3 cut(s) | 2233, 2435, 3458 |
| ApoI | RAATTY | 8 cut(s) | 7, 745, 765, 1395, 1675, 3132, 3394, 3612 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1056, 1088 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 465, 1074, 3626 |
| AspLEI | GCGC | 2 cut(s) | 2609, 3673 |
| AspS9I | GGNCC | 2 cut(s) | 220, 968 |
| AsuC2I | CCSGG | 2 cut(s) | 3455, 3619 |
| AsuHPI | GGTGA | 9 cut(s) | 470, 589, 628, 1202, 1935, 2278, 2564, 2592, 2828 |
| AsuII | TTCGAA | 1 cut(s) | 743 |
| AsuNHI | GCTAGC | 1 cut(s) | 2785 |
| AvaI | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| AvaII | GGWCC | 2 cut(s) | 220, 968 |
| BalI | TGGCCA | 2 cut(s) | 884, 2452 |
| BamHI | GGATCC | 1 cut(s) | 2170 |
| BanI | GGYRCC | 1 cut(s) | 1828 |
| BanII | GRGCYC | 2 cut(s) | 984, 3004 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2633, 2665 |
| BauI | CACGAG | 2 cut(s) | 1287, 2237 |
| BbsI | GAAGAC | 3 cut(s) | 414, 1866, 2513 |
| Bbv12I | GWGCWC | 3 cut(s) | 721, 1309, 3004 |
| BbvCI | CCTCAGC | 2 cut(s) | 2502, 3339 |
| BbvI | GCAGC | 3 cut(s) | 2245, 2422, 3470 |
| BccI | CCATC | 7 cut(s) | 690, 805, 1381, 1792, 2197, 2300, 2758 |
| BceAI | ACGGC | 2 cut(s) | 1322, 1417 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 3650 |
| BciT130I | CCWGG | 8 cut(s) | 640, 714, 1826, 1911, 2529, 2550, 2851, 3159 |
| BciVI | GTATCC | 1 cut(s) | 445 |
| BclI | TGATCA | 1 cut(s) | 382 |
| BcnI | CCSGG | 2 cut(s) | 3455, 3619 |
| BcoDI | GTCTC | 7 cut(s) | 337, 994, 1429, 2083, 2375, 2594, 2903 |
| BfmI | CTRYAG | 2 cut(s) | 285, 2265 |
| BfuAI | ACCTGC | 1 cut(s) | 2907 |
| BfuI | GTATCC | 1 cut(s) | 445 |
| BglII | AGATCT | 1 cut(s) | 1690 |
| BisI | GCNGC | 3 cut(s) | 2234, 2436, 3459 |
| BlsI | GCNGC | 3 cut(s) | 2235, 2437, 3460 |
| BmcAI | AGTACT | 3 cut(s) | 1598, 2828, 3430 |
| Bme18I | GGWCC | 2 cut(s) | 220, 968 |
| BmeT110I | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| BmgBI | CACGTC | 1 cut(s) | 1727 |
| BmgT120I | GGNCC | 2 cut(s) | 220, 968 |
| BmiI | GGNNCC | 3 cut(s) | 969, 1830, 2172 |
| BmrI | ACTGGG | 1 cut(s) | 2057 |
| BmtI | GCTAGC | 1 cut(s) | 2789 |
| BmuI | ACTGGG | 1 cut(s) | 2057 |
| BpiI | GAAGAC | 3 cut(s) | 414, 1866, 2513 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2563, 2595 |
| BpmI | CTGGAG | 3 cut(s) | 723, 1218, 3141 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2502, 3339 |
| Bpu14I | TTCGAA | 1 cut(s) | 743 |
| BpuEI | CTTGAG | 2 cut(s) | 2669, 3218 |
| BpuMI | CCSGG | 2 cut(s) | 3455, 3619 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1695 |
| BsaI | GGTCTC | 2 cut(s) | 337, 2594 |
| BsaJI | CCNNGG | 5 cut(s) | 639, 1909, 2528, 2850, 3158 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 892, 922, 1149, 1179, 2374, 2404, 2530, 2560 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 476, 2252, 2983, 3164 |
| Bse1I | ACTGG | 7 cut(s) | 431, 487, 1832, 2063, 2453, 2749, 3482 |
| Bse3DI | GCAATG | 3 cut(s) | 107, 2196, 2755 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1695 |
| BseBI | CCWGG | 8 cut(s) | 640, 714, 1826, 1911, 2529, 2550, 2851, 3159 |
| BseDI | CCNNGG | 5 cut(s) | 639, 1909, 2528, 2850, 3158 |
| BseGI | GGATG | 7 cut(s) | 793, 1519, 1618, 1821, 2164, 2889, 3124 |
| BseJI | GATNNNNATC | 1 cut(s) | 1695 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 476, 2252, 2983, 3164 |
| BseMI | GCAATG | 3 cut(s) | 107, 2196, 2755 |
| BseNI | ACTGG | 7 cut(s) | 431, 487, 1832, 2063, 2453, 2749, 3482 |
| BseRI | GAGGAG | 4 cut(s) | 1639, 2235, 2519, 3180 |
| BseXI | GCAGC | 3 cut(s) | 2245, 2422, 3470 |
| Bsh1236I | CGCG | 1 cut(s) | 2607 |
| BshFI | GGCC | 3 cut(s) | 884, 1475, 2452 |
| BshNI | GGYRCC | 1 cut(s) | 1828 |
| BsiHKAI | GWGCWC | 3 cut(s) | 721, 1309, 3004 |
| BsiHKCI | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| BsiSI | CCGG | 2 cut(s) | 3454, 3619 |
| BslFI | GGGAC | 6 cut(s) | 493, 518, 548, 709, 981, 1856 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 476, 2252, 2983, 3164 |
| BsmAI | GTCTC | 7 cut(s) | 337, 994, 1429, 2083, 2375, 2594, 2903 |
| BsmFI | GGGAC | 6 cut(s) | 493, 518, 548, 709, 981, 1856 |
| BsmI | GAATGC | 1 cut(s) | 2482 |
| BsnI | GGCC | 3 cut(s) | 884, 1475, 2452 |
| Bso31I | GGTCTC | 2 cut(s) | 337, 2594 |
| BsoBI | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| Bsp119I | TTCGAA | 1 cut(s) | 743 |
| Bsp1286I | GDGCHC | 4 cut(s) | 721, 984, 1309, 3004 |
| Bsp1407I | TGTACA | 1 cut(s) | 211 |
| BspACI | CCGC | 4 cut(s) | 158, 727, 732, 2638 |
| BspANI | GGCC | 3 cut(s) | 884, 1475, 2452 |
| BspFNI | CGCG | 1 cut(s) | 2607 |
| BspLI | GGNNCC | 3 cut(s) | 969, 1830, 2172 |
| BspMI | ACCTGC | 1 cut(s) | 2907 |
| BspOI | GCTAGC | 1 cut(s) | 2789 |
| BspPI | GGATC | 5 cut(s) | 493, 636, 2165, 2178, 2317 |
| BspQI | GCTCTTC | 2 cut(s) | 1056, 3576 |
| BspT104I | TTCGAA | 1 cut(s) | 743 |
| BspT107I | GGYRCC | 1 cut(s) | 1828 |
| BspTNI | GGTCTC | 2 cut(s) | 337, 2594 |
| BsrDI | GCAATG | 3 cut(s) | 107, 2196, 2755 |
| BsrGI | TGTACA | 1 cut(s) | 211 |
| BsrI | ACTGG | 7 cut(s) | 431, 487, 1832, 2063, 2453, 2749, 3482 |
| BssECI | CCNNGG | 5 cut(s) | 639, 1909, 2528, 2850, 3158 |
| BssNAI | GTATAC | 1 cut(s) | 2512 |
| BssSI | CACGAG | 2 cut(s) | 1287, 2237 |
| Bst1107I | GTATAC | 1 cut(s) | 2512 |
| Bst2BI | CACGAG | 2 cut(s) | 1287, 2237 |
| Bst2UI | CCWGG | 8 cut(s) | 640, 714, 1826, 1911, 2529, 2550, 2851, 3159 |
| Bst6I | CTCTTC | 4 cut(s) | 1056, 2193, 3296, 3576 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 296, 2239 |
| BstAUI | TGTACA | 1 cut(s) | 211 |
| BstBI | TTCGAA | 1 cut(s) | 743 |
| BstC8I | GCNNGC | 3 cut(s) | 2332, 2787, 3004 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 474, 2250 |
| BstF5I | GGATG | 7 cut(s) | 793, 1519, 1618, 1821, 2164, 2889, 3124 |
| BstFNI | CGCG | 1 cut(s) | 2607 |
| BstHHI | GCGC | 2 cut(s) | 2609, 3673 |
| BstMAI | GTCTC | 7 cut(s) | 337, 994, 1429, 2083, 2375, 2594, 2903 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 157, 203, 296, 1343, 2239, 2396, 3227, 3651 |
| BstNI | CCWGG | 8 cut(s) | 640, 714, 1826, 1911, 2529, 2550, 2851, 3159 |
| BstNSI | RCATGY | 4 cut(s) | 131, 876, 1224, 3509 |
| BstSFI | CTRYAG | 2 cut(s) | 285, 2265 |
| BstUI | CGCG | 1 cut(s) | 2607 |
| BstV1I | GCAGC | 3 cut(s) | 2245, 2422, 3470 |
| BstV2I | GAAGAC | 3 cut(s) | 414, 1866, 2513 |
| BstX2I | RGATCY | 3 cut(s) | 628, 1690, 2170 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1199 |
| BstYI | RGATCY | 3 cut(s) | 628, 1690, 2170 |
| BstZ17I | GTATAC | 1 cut(s) | 2512 |
| BsuI | GTATCC | 1 cut(s) | 445 |
| BsuRI | GGCC | 3 cut(s) | 884, 1475, 2452 |
| BtgZI | GCGATG | 2 cut(s) | 764, 1347 |
| BtrI | CACGTC | 1 cut(s) | 1727 |
| BtsCI | GGATG | 7 cut(s) | 793, 1519, 1618, 1821, 2164, 2889, 3124 |
| BtsI | GCAGTG | 1 cut(s) | 3075 |
| BtsIMutI | CAGTG | 6 cut(s) | 576, 984, 1842, 2037, 2742, 3075 |
| BveI | ACCTGC | 1 cut(s) | 2907 |
| Cac8I | GCNNGC | 3 cut(s) | 2332, 2787, 3004 |
| CfoI | GCGC | 2 cut(s) | 2609, 3673 |
| Cfr13I | GGNCC | 2 cut(s) | 220, 968 |
| CseI | GACGC | 1 cut(s) | 3227 |
| DraI | TTTAAA | 2 cut(s) | 327, 2445 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1868 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1868 |
| EaeI | YGGCCR | 2 cut(s) | 882, 2450 |
| Eam1104I | CTCTTC | 4 cut(s) | 1056, 2193, 3296, 3576 |
| EarI | CTCTTC | 4 cut(s) | 1056, 2193, 3296, 3576 |
| Ecl136II | GAGCTC | 1 cut(s) | 3002 |
| Eco24I | GRGCYC | 2 cut(s) | 984, 3004 |
| Eco31I | GGTCTC | 2 cut(s) | 337, 2594 |
| Eco32I | GATATC | 2 cut(s) | 558, 1556 |
| Eco47I | GGWCC | 2 cut(s) | 220, 968 |
| Eco53kI | GAGCTC | 1 cut(s) | 3002 |
| Eco57I | CTGAAG | 4 cut(s) | 1370, 1649, 1878, 3600 |
| Eco88I | CYCGRG | 3 cut(s) | 991, 1518, 2007 |
| EcoICRI | GAGCTC | 1 cut(s) | 3002 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 474, 2250 |
| EcoO109I | RGGNCCY | 1 cut(s) | 968 |
| EcoRI | GAATTC | 2 cut(s) | 745, 1675 |
| EcoRII | CCWGG | 8 cut(s) | 638, 712, 1824, 1909, 2527, 2548, 2849, 3157 |
| EcoRV | GATATC | 2 cut(s) | 558, 1556 |
| EcoT22I | ATGCAT | 4 cut(s) | 2110, 2164, 2482, 3096 |
| EcoT38I | GRGCYC | 2 cut(s) | 984, 3004 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 2319, 2351, 2816, 2848, 3525, 3557 |
| FaqI | GGGAC | 6 cut(s) | 493, 518, 548, 709, 981, 1856 |
| FauI | CCCGC | 2 cut(s) | 165, 734 |
| FauNDI | CATATG | 3 cut(s) | 387, 1311, 2104 |
| FbaI | TGATCA | 1 cut(s) | 382 |
| FblI | GTMKAC | 3 cut(s) | 2003, 2511, 2990 |
| Fnu4HI | GCNGC | 3 cut(s) | 2234, 2436, 3459 |
| FokI | GGATG | 7 cut(s) | 780, 1526, 1625, 1808, 2171, 2876, 3131 |
| FriOI | GRGCYC | 2 cut(s) | 984, 3004 |
| Fsp4HI | GCNGC | 3 cut(s) | 2234, 2436, 3459 |
| GlaI | GCGC | 2 cut(s) | 2608, 3672 |
| GluI | GCNGC | 3 cut(s) | 2234, 2436, 3459 |
| GsuI | CTGGAG | 3 cut(s) | 723, 1218, 3141 |
| HaeIII | GGCC | 3 cut(s) | 884, 1475, 2452 |
| HapII | CCGG | 2 cut(s) | 3454, 3619 |
| HgaI | GACGC | 1 cut(s) | 3227 |
| HhaI | GCGC | 2 cut(s) | 2609, 3673 |
| Hin6I | GCGC | 2 cut(s) | 2607, 3671 |
| HinP1I | GCGC | 2 cut(s) | 2607, 3671 |
| HincII | GTYRAC | 3 cut(s) | 1771, 2015, 3255 |
| HindII | GTYRAC | 3 cut(s) | 1771, 2015, 3255 |
| HindIII | AAGCTT | 3 cut(s) | 462, 1344, 3539 |
| HpaII | CCGG | 2 cut(s) | 3454, 3619 |
| HphI | GGTGA | 9 cut(s) | 470, 589, 628, 1202, 1935, 2278, 2564, 2592, 2828 |
| Hpy188III | TCNNGA | 7 cut(s) | 626, 686, 975, 1235, 1793, 2473, 3197 |
| HpyAV | CCTTC | 9 cut(s) | 981, 1379, 1394, 2179, 2254, 2695, 2800, 2855, 3616 |
| HpyCH4IV | ACGT | 2 cut(s) | 1726, 2360 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 157, 203, 296, 1343, 2239, 2396, 3227, 3651 |
| HpySE526I | ACGT | 2 cut(s) | 1726, 2360 |
| HspAI | GCGC | 2 cut(s) | 2607, 3671 |
| Ksp22I | TGATCA | 1 cut(s) | 382 |
| LguI | GCTCTTC | 2 cut(s) | 1056, 3576 |
| LmnI | GCTCC | 7 cut(s) | 643, 716, 1819, 1891, 1911, 2395, 3160 |
| Lsp1109I | GCAGC | 3 cut(s) | 2245, 2422, 3470 |
| MaeII | ACGT | 2 cut(s) | 1726, 2360 |
| MfeI | CAATTG | 2 cut(s) | 215, 569 |
| MflI | RGATCY | 3 cut(s) | 628, 1690, 2170 |
| MhlI | GDGCHC | 4 cut(s) | 721, 984, 1309, 3004 |
| MlsI | TGGCCA | 2 cut(s) | 884, 2452 |
| MluNI | TGGCCA | 2 cut(s) | 884, 2452 |
| MlyI | GAGTC | 7 cut(s) | 860, 1143, 1771, 1976, 2527, 2668, 3629 |
| MmeI | TCCRAC | 5 cut(s) | 55, 125, 1491, 1957, 2901 |
| Mox20I | TGGCCA | 2 cut(s) | 884, 2452 |
| Mph1103I | ATGCAT | 4 cut(s) | 2110, 2164, 2482, 3096 |
| MroXI | GAANNNNTTC | 3 cut(s) | 465, 1074, 3626 |
| MscI | TGGCCA | 2 cut(s) | 884, 2452 |
| MseI | TTAA | 9 cut(s) | 17, 165, 326, 737, 947, 1167, 1527, 2444, 3137 |
| MslI | CAYNNNNRTG | 3 cut(s) | 614, 957, 3117 |
| Msp20I | TGGCCA | 2 cut(s) | 884, 2452 |
| MspI | CCGG | 2 cut(s) | 3454, 3619 |
| MunI | CAATTG | 2 cut(s) | 215, 569 |
| Mva1269I | GAATGC | 1 cut(s) | 2482 |
| MvaI | CCWGG | 8 cut(s) | 640, 714, 1826, 1911, 2529, 2550, 2851, 3159 |
| MvnI | CGCG | 1 cut(s) | 2607 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 157, 203, 296, 1343, 2239, 2396, 3227, 3651 |
| NciI | CCSGG | 2 cut(s) | 3455, 3619 |
| NdeI | CATATG | 3 cut(s) | 387, 1311, 2104 |
| NheI | GCTAGC | 1 cut(s) | 2785 |
| NlaIV | GGNNCC | 3 cut(s) | 969, 1830, 2172 |
| NmuCI | GTSAC | 7 cut(s) | 1603, 1735, 2598, 2660, 3110, 3446, 3521 |
| NsiI | ATGCAT | 4 cut(s) | 2110, 2164, 2482, 3096 |
| NspI | RCATGY | 4 cut(s) | 131, 876, 1224, 3509 |
| NspV | TTCGAA | 1 cut(s) | 743 |
| PaeR7I | CTCGAG | 1 cut(s) | 2007 |
| PciI | ACATGT | 1 cut(s) | 872 |
| PciSI | GCTCTTC | 2 cut(s) | 1056, 3576 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1264 |
| PctI | GAATGC | 1 cut(s) | 2482 |
| PdmI | GAANNNNTTC | 3 cut(s) | 465, 1074, 3626 |
| PfeI | GAWTC | 7 cut(s) | 368, 581, 1789, 1853, 2119, 2865, 3478 |
| PflFI | GACNNNGTC | 1 cut(s) | 2658 |
| PfoI | TCCNGGA | 1 cut(s) | 3617 |
| PkrI | GCNGC | 3 cut(s) | 2235, 2437, 3460 |
| PleI | GAGTC | 7 cut(s) | 859, 1142, 1770, 1975, 2526, 2667, 3629 |
| PpsI | GAGTC | 7 cut(s) | 859, 1142, 1770, 1975, 2526, 2667, 3629 |
| PpuMI | RGGWCCY | 1 cut(s) | 968 |
| PscI | ACATGT | 1 cut(s) | 872 |
| PsiI | TTATAA | 2 cut(s) | 1361, 3530 |
| Psp124BI | GAGCTC | 1 cut(s) | 3004 |
| Psp5II | RGGWCCY | 1 cut(s) | 968 |
| Psp6I | CCWGG | 8 cut(s) | 638, 712, 1824, 1909, 2527, 2548, 2849, 3157 |
| PspGI | CCWGG | 8 cut(s) | 638, 712, 1824, 1909, 2527, 2548, 2849, 3157 |
| PspN4I | GGNNCC | 3 cut(s) | 969, 1830, 2172 |
| PspPI | GGNCC | 2 cut(s) | 220, 968 |
| PspPPI | RGGWCCY | 1 cut(s) | 968 |
| PspXI | VCTCGAGB | 1 cut(s) | 2007 |
| PsuI | RGATCY | 3 cut(s) | 628, 1690, 2170 |
| PsyI | GACNNNGTC | 1 cut(s) | 2658 |
| RseI | CAYNNNNRTG | 3 cut(s) | 614, 957, 3117 |
| SacI | GAGCTC | 1 cut(s) | 3004 |
| SapI | GCTCTTC | 2 cut(s) | 1056, 3576 |
| SaqAI | TTAA | 9 cut(s) | 17, 165, 326, 737, 947, 1167, 1527, 2444, 3137 |
| SatI | GCNGC | 3 cut(s) | 2234, 2436, 3459 |
| Sau96I | GGNCC | 2 cut(s) | 220, 968 |
| ScaI | AGTACT | 3 cut(s) | 1598, 2828, 3430 |
| SchI | GAGTC | 7 cut(s) | 860, 1143, 1771, 1976, 2527, 2668, 3629 |
| SduI | GDGCHC | 4 cut(s) | 721, 984, 1309, 3004 |
| SfcI | CTRYAG | 2 cut(s) | 285, 2265 |
| Sfr274I | CTCGAG | 1 cut(s) | 2007 |
| SfuI | TTCGAA | 1 cut(s) | 743 |
| SinI | GGWCC | 2 cut(s) | 220, 968 |
| SlaI | CTCGAG | 1 cut(s) | 2007 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 614, 957, 3117 |
| SmlI | CTYRAG | 3 cut(s) | 2007, 2684, 3197 |
| SmoI | CTYRAG | 3 cut(s) | 2007, 2684, 3197 |
| SsiI | CCGC | 4 cut(s) | 158, 727, 732, 2638 |
| SstI | GAGCTC | 1 cut(s) | 3004 |
| TaiI | ACGT | 2 cut(s) | 1729, 2363 |
| TaqI | TCGA | 7 cut(s) | 133, 743, 1787, 2008, 2458, 2998, 3660 |
| TatI | WGTACW | 8 cut(s) | 211, 1028, 1596, 2152, 2826, 2886, 3428, 3484 |
| TfiI | GAWTC | 7 cut(s) | 368, 581, 1789, 1853, 2119, 2865, 3478 |
| Tru1I | TTAA | 9 cut(s) | 17, 165, 326, 737, 947, 1167, 1527, 2444, 3137 |
| Tru9I | TTAA | 9 cut(s) | 17, 165, 326, 737, 947, 1167, 1527, 2444, 3137 |
| TscAI | CASTG | 6 cut(s) | 583, 991, 1849, 2044, 2749, 3075 |
| TseFI | GTSAC | 7 cut(s) | 1603, 1735, 2598, 2660, 3110, 3446, 3521 |
| TseI | GCWGC | 3 cut(s) | 2233, 2435, 3458 |
| Tsp45I | GTSAC | 7 cut(s) | 1603, 1735, 2598, 2660, 3110, 3446, 3521 |
| TspGWI | ACGGA | 2 cut(s) | 482, 1273 |
| TspRI | CASTG | 6 cut(s) | 583, 991, 1849, 2044, 2749, 3075 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2658 |
| VpaK11BI | GGWCC | 2 cut(s) | 220, 968 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 474, 2250 |
| XapI | RAATTY | 8 cut(s) | 7, 745, 765, 1395, 1675, 3132, 3394, 3612 |
| XbaI | TCTAGA | 2 cut(s) | 685, 974 |
| XceI | RCATGY | 4 cut(s) | 131, 876, 1224, 3509 |
| XhoI | CTCGAG | 1 cut(s) | 2007 |
| XmiI | GTMKAC | 3 cut(s) | 2003, 2511, 2990 |
| XmnI | GAANNNNTTC | 3 cut(s) | 465, 1074, 3626 |
| ZrmI | AGTACT | 3 cut(s) | 1598, 2828, 3430 |
| Zsp2I | ATGCAT | 4 cut(s) | 2110, 2164, 2482, 3096 |