Rw2G031560

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000071
Physical Location & Seq
Reverse (-)
51699466 .. 51703112
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031560.1

Sequence Viewer

Length: 2796 bp
ATGTCAAAGGAAATTTCTGGCAGTGTCATCCATTGCAAGGATGCAAGAAGCATGTGGCTTGAATTGCAGGAAAGGTTCTCGCATACAAACACCGTGCAACTTTTCCATATAGAAAACGCCATTCATGATTGTGAACAAGGCGGTACCTCAGTGACATCTTTCTTCACCAAACTCAAGACTCTCTGGGATGAGAAAGATGCCTTATCCAAGGAAATCAAGGATTACTTGGAGACACAAAAGACAATGAAATTTTTGATGGGTCTCACTGACAATTATGCCACGATTCGAAGCAATATTGTTGGGATGGATCCTCTTCCGAATGTGAACAAGGCTTACTCCATGTCACTTCGACATGAAAAGCAAGCAGAGGTGTCAAGTGGCAAGATGTCTACTCAGCCGGAGGCGGCTGCCTTTGCTGTGAAGAGAACCAACCGTGAGTTTGATCTTGGAGAAAGTGAGACTAAATGTGAAAAATGCAACAAAAATAATCACAGCACAAAGAATTGCAAGGCACACCTCAAGTGTACATTCTGCAATTGGAAAGAGCACACTTACGAGTACTGCCGCAAGAGGAAGGCTGCCATGGAGGGAGGACAAGTGCGTGCGAAAGGAAATCATGTGCTCTCCATGCATGATAAACAAGAAGTTGGACCCAGTTTTCCATTTTCACAAGAAGAGTGTCAACAGCTTATGAACCTCTTAAACAAAAACAAACCTGCCACAGCAAATCAAGTTGGTAATGTTCCAAACTTTGATGAAATCTCAGGTAAAGCATTCACTCTCTCTCGAGATGGGAAAGAAACAATATGGATATTGGATAGTGGAGCTAGTGATCACATAACAAGTTTGTGTCATACAGGACCTACGATCGGGAAGATGATTGGGATGGGAACTGAGAGGGAGGGACTCTACTGCCTTGTTCCCACGAAGAAAAGTTCATGTAATTCCATTCAAAGCAAACCACACATCCTCTGGCATCAACGACTCGGTCACCCTTCCACCAAATCTTCGTTTGATTTGATACATGTTGACATTTGGGGTGGATATCATGTTCCTTCTATTTCTGGTGCCCAATATTTTCTCACCATAGTTGATGATCACACTAGGTGCACTTGGGTATATTTGATGAAAAATAAATCAAAAACACGAACTCTCCTAGTTAACTACATTCATATGGTTGAAAACCAGTTTGGTAAAAGGGTAAAAGTTGTCCTTGTTTCCAGAGACGTTTTATTCTTTGAGGATATCTTTCCATTTCAGTCCAACACCAAAATAAATTCTCCATCCACCATGCCTATTTTTCCGCAATCCTCAAATCTCGATTTTGATCTTCCAGATTTACCTCCATCTCATCAGGAAGATTCTATTCCTGAAAAGTCTCCTCCTGATCCTACTTCCGCTGCAAGTCAACCTCTTTCTCACACCACCAGTCCTTCTTCATCGGACTTAACCATTCCTTCTGCCACTGAACACTCTCCTCCTTCACAAACCGAGACAACCATTCTGCCTCAACCACGGTCCTCACCACCACCACGATCTTCCAGGCCAACCAAAGTTCCCTCATATTTGCAGGATTTCCATGTAGAGGCTGCTCTCCCCTCACGACTCACCCAATCACCTTCAACGAACGTGGTTCTTCACTTCAGTACCGCTTATCCTCTTTCTCATGTCTTATCTTATGATAAACTCTCTTCTCACCATGGAGTCTTCACCACTAAACTTACCCTTCTGCGAGAACCTACTTCTTTTACCCAAGCAGAGAGTCCACAATGGAGAGAGGCCATGCACAAGGAGATTCAAGCACTCCAAGCCAACAATACTTGGAGTTTGGTGCCCTTACCACCACATAAACGGCCCATTGGCTGCAAATGGGTTTACAAGATCAAACTCAATCCTGATGGTACCGTTGAGAGATACAAAGCACGCCTAGTTGCTAAGGGCTACAGTCAAATTGAAGGGCTTGATTATCGTGAGACTTTCGCCCCTGTTGCAAAATTGACAACCGTCAGAGTCCTCCTCAGTCTCGCCGCTCTACAAGGTTGGCATTTACACCAATTGGACGTGAACAATGCGTTTCTTCATGGTGATCTTTATGAAGACGTATACATGCATATACCTCCTGGGTTTGGCCGAAAGGGGGAGACTAGGGTTTGCAAGCTACACAAGTCGCTCAATGGCTTAAAACAAGCATCACGACAATGGTTCATTAAGCTCTCAACAGCTCTCAAGTCAGGAGGATTTCATCAATCTTGGTCTGATTATTCCCTATTCGTCCGAACACATCAAGGAAAATTCACGGCCTTATTGGTTTATGTTGATGATGTAATCCTGGCAGGAAATAGTTTGAAGGACATCATAGACACCAAACAATTCCTTACCAGTCATTTCAAGCTAAAGGACCAAGGGATTGCATTATGTCAGAGGAAGTATGCCCTAGAGATTCTTGAAGACACCGGTTTCCTTGGTGCCAAAGCATCACGTTTCCCTGTTGATCAAAGTCTATCACTCACACAAACAAATGGAGATTTACTAACTGATCCCTCACAATATCGCAGGTTAGTAGGACGGCTCATATATCTCACCATCACCAGACCTGATCTTGTCTATGCAGTCCACTTACTAAGTCAGTTTATGGATAAGCCCAGGCAACCACATCTTGATGCAGCACATAAGGTCTTAAGGTACCTTAAACAAGCACCAGGTCAAGGGATTCTTCTGCCTTCCACGGGGTCCTTAGAGTTAAAGGCATATTGCGACGCGCAATATGCTGACTGGGCTCGTTGCAAGGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

931

Amino Acids

104.89

Weight (kDa)

8.94

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 1 - 124 4.4e-06 gag-polypeptide of LTR copia-type
RVT_2 PF07727 605 - 819 1.2e-60 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 724, 2544
Acc65I GGTACC 3 cut(s) 143, 1901, 2682
AccB1I GGYRCC 6 cut(s) 143, 1067, 1831, 1901, 2465, 2682
AccBSI CCGCTC 1 cut(s) 2030
AccI GTMKAC 2 cut(s) 389, 2103
AccII CGCG 1 cut(s) 2759
AciI CCGC 7 cut(s) 141, 404, 565, 1304, 1398, 1650, 2028
AclWI GGATC 4 cut(s) 302, 315, 1382, 2531
AcoI YGGCCR 1 cut(s) 2128
AcsI RAATTY 4 cut(s) 12, 248, 1276, 2291
AcuI CTGAAG 1 cut(s) 1627
AdeI CACNNNGTG 1 cut(s) 1107
AfaI GTAC 6 cut(s) 145, 526, 560, 1648, 1903, 2684
AfiI CCNNNNNNNGG 7 cut(s) 37, 869, 1585, 2126, 2137, 2705, 2726
AflII CTTAAG 1 cut(s) 2677
AflIII ACRYGT 1 cut(s) 1024
AgeI ACCGGT 1 cut(s) 2453
AgsI TTSAA 9 cut(s) 62, 953, 1181, 1623, 1799, 1955, 2347, 2389, 2447
AjiI CACGTC 1 cut(s) 2062
AjnI CCWGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 1173, 1205
AloI GAACNNNNNNTCC 2 cut(s) 1035, 1067
AluBI AGCT 6 cut(s) 688, 827, 2158, 2212, 2222, 2392
AluI AGCT 6 cut(s) 688, 827, 2158, 2212, 2222, 2392
Alw21I GWGCWC 3 cut(s) 549, 624, 1112
Alw26I GTCTC 9 cut(s) 224, 266, 452, 1218, 1383, 1487, 1967, 2027, 2135
Alw44I GTGCAC 1 cut(s) 1108
AlwI GGATC 4 cut(s) 302, 315, 1382, 2531
Ama87I CYCGRG 1 cut(s) 786
AoxI GGCC 5 cut(s) 1544, 1779, 1853, 2128, 2298
ApaLI GTGCAC 1 cut(s) 1108
ApeKI GCWGC 6 cut(s) 407, 578, 1400, 1589, 1863, 2663
ApoI RAATTY 4 cut(s) 12, 248, 1276, 2291
AsiGI ACCGGT 1 cut(s) 2453
Asp718I GGTACC 3 cut(s) 143, 1901, 2682
AspLEI GCGC 1 cut(s) 2761
AspS9I GGNCC 6 cut(s) 650, 860, 1518, 1854, 2398, 2730
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 5 cut(s) 650, 860, 1518, 2398, 2730
BaeGI GKGCMC 3 cut(s) 1072, 1112, 1836
BaeI ACNNNNGTAYC 4 cut(s) 135, 168, 1630, 1663
BamHI GGATCC 1 cut(s) 307
BanI GGYRCC 6 cut(s) 143, 1067, 1831, 1901, 2465, 2682
BanII GRGCYC 1 cut(s) 2779
BbsI GAAGAC 3 cut(s) 1699, 2103, 2454
Bbv12I GWGCWC 3 cut(s) 549, 624, 1112
BbvI GCAGC 6 cut(s) 394, 565, 1387, 1576, 1850, 2675
BccI CCATC 8 cut(s) 250, 298, 785, 880, 1291, 1354, 1892, 2591
BceAI ACGGC 3 cut(s) 1868, 2313, 2582
BciT130I CCWGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
BciVI GTATCC 1 cut(s) 2782
BclI TGATCA 3 cut(s) 832, 1096, 2491
BcoDI GTCTC 9 cut(s) 224, 266, 452, 1218, 1383, 1487, 1967, 2027, 2135
BfaI CTAG 6 cut(s) 828, 1104, 1157, 1928, 2145, 2435
BfmI CTRYAG 1 cut(s) 1942
BfrI CTTAAG 1 cut(s) 2677
BfuAI ACCTGC 2 cut(s) 724, 2544
BfuI GTATCC 1 cut(s) 2782
BisI GCNGC 9 cut(s) 405, 408, 565, 579, 1401, 1590, 1864, 2028, 2664
BlsI GCNGC 9 cut(s) 406, 409, 566, 580, 1402, 1591, 1865, 2029, 2665
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
Bme18I GGWCC 5 cut(s) 650, 860, 1518, 2398, 2730
BmeT110I CYCGRG 1 cut(s) 786
BmgBI CACGTC 1 cut(s) 2062
BmgT120I GGNCC 6 cut(s) 650, 860, 1518, 1854, 2398, 2730
BmiI GGNNCC 9 cut(s) 145, 309, 652, 1069, 1833, 1903, 2467, 2684, 2731
BmrFI CCNGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
BmrI ACTGGG 2 cut(s) 648, 2782
BmsI GCATC 6 cut(s) 31, 187, 985, 2198, 2483, 2650
BmuI ACTGGG 2 cut(s) 648, 2782
BoxI GACNNNNGTC 1 cut(s) 2003
BpiI GAAGAC 3 cut(s) 1699, 2103, 2454
BplI GAGNNNNNCTC 2 cut(s) 2001, 2033
Bpu10I CCTNAGC 1 cut(s) 1935
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 3 cut(s) 158, 503, 2210
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 9 cut(s) 207, 582, 1514, 1699, 2120, 2401, 2461, 2642, 2724
BsaWI WCCGGW 1 cut(s) 2453
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 1137, 1167
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 869, 1585, 2126, 2137, 2705, 2726
Bse118I RCCGGY 1 cut(s) 2453
Bse1I ACTGG 5 cut(s) 654, 1186, 1428, 2379, 2777
Bse3DI GCAATG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 1326
BseBI CCWGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
BseDI CCNNGG 9 cut(s) 207, 582, 1514, 1699, 2120, 2401, 2461, 2642, 2724
BseGI GGATG 7 cut(s) 27, 46, 193, 309, 891, 966, 1283
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 7 cut(s) 37, 869, 1585, 2126, 2137, 2705, 2726
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 5 cut(s) 162, 407, 777, 885, 2032
BseNI ACTGG 5 cut(s) 654, 1186, 1428, 2379, 2777
BseRI GAGGAG 3 cut(s) 1371, 1467, 2006
BseSI GKGCMC 3 cut(s) 1072, 1112, 1836
BseXI GCAGC 6 cut(s) 394, 565, 1387, 1576, 1850, 2675
Bsh1236I CGCG 1 cut(s) 2759
Bsh1285I CGRYCG 1 cut(s) 870
BshFI GGCC 5 cut(s) 1546, 1781, 1855, 2130, 2300
BshNI GGYRCC 6 cut(s) 143, 1067, 1831, 1901, 2465, 2682
BshTI ACCGGT 1 cut(s) 2453
BsiEI CGRYCG 1 cut(s) 870
BsiHKAI GWGCWC 3 cut(s) 549, 624, 1112
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 2 cut(s) 398, 2454
BslFI GGGAC 1 cut(s) 918
BslI CCNNNNNNNGG 7 cut(s) 37, 869, 1585, 2126, 2137, 2705, 2726
BsmAI GTCTC 9 cut(s) 224, 266, 452, 1218, 1383, 1487, 1967, 2027, 2135
BsmBI CGTCTC 1 cut(s) 1218
BsmFI GGGAC 1 cut(s) 918
BsmI GAATGC 1 cut(s) 773
BsnI GGCC 5 cut(s) 1546, 1781, 1855, 2130, 2300
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 786
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 6 cut(s) 549, 624, 1072, 1112, 1836, 2779
Bsp1407I TGTACA 1 cut(s) 524
Bsp19I CCATGG 2 cut(s) 582, 1699
BspACI CCGC 7 cut(s) 141, 404, 565, 1304, 1398, 1650, 2028
BspANI GGCC 5 cut(s) 1546, 1781, 1855, 2130, 2300
BspCNI CTCAG 5 cut(s) 161, 406, 776, 886, 2031
BspFNI CGCG 1 cut(s) 2759
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 9 cut(s) 145, 309, 652, 1069, 1833, 1903, 2467, 2684, 2731
BspMI ACCTGC 2 cut(s) 724, 2544
BspPI GGATC 4 cut(s) 302, 315, 1382, 2531
BspT104I TTCGAA 1 cut(s) 286
BspT107I GGYRCC 6 cut(s) 143, 1067, 1831, 1901, 2465, 2682
BspTI CTTAAG 1 cut(s) 2677
BspTNI GGTCTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 2030
BsrDI GCAATG 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 2453
BsrGI TGTACA 1 cut(s) 524
BsrI ACTGG 5 cut(s) 654, 1186, 1428, 2379, 2777
BssAI RCCGGY 1 cut(s) 2453
BssECI CCNNGG 9 cut(s) 207, 582, 1514, 1699, 2120, 2401, 2461, 2642, 2724
BssNAI GTATAC 1 cut(s) 2104
BssT1I CCWWGG 5 cut(s) 207, 582, 1699, 2401, 2461
Bst1107I GTATAC 1 cut(s) 2104
Bst2UI CCWGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
Bst4CI ACNGT 6 cut(s) 94, 434, 1518, 1906, 1946, 2005
Bst6I CTCTTC 4 cut(s) 318, 416, 669, 1696
BstAFI CTTAAG 1 cut(s) 2677
BstAUI TGTACA 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 4 cut(s) 363, 603, 1924, 2156
BstDEI CTNAG 8 cut(s) 148, 393, 763, 894, 1935, 2018, 2621, 2734
BstDSI CCRYGG 4 cut(s) 582, 1514, 1699, 2724
BstEII GGTNACC 1 cut(s) 989
BstF5I GGATG 7 cut(s) 27, 46, 193, 309, 891, 966, 1283
BstFNI CGCG 1 cut(s) 2759
BstHHI GCGC 1 cut(s) 2761
BstMAI GTCTC 9 cut(s) 224, 266, 452, 1218, 1383, 1487, 1967, 2027, 2135
BstMCI CGRYCG 1 cut(s) 870
BstMWI GCNNNNNNNGC 7 cut(s) 64, 413, 628, 1808, 1988, 2765, 2774
BstNI CCWGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
BstNSI RCATGY 3 cut(s) 55, 1028, 2110
BstPAI GACNNNNGTC 1 cut(s) 2003
BstPI GGTNACC 1 cut(s) 989
BstSCI CCNGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
BstSFI CTRYAG 1 cut(s) 1942
BstSLI GKGCMC 3 cut(s) 1072, 1112, 1836
BstUI CGCG 1 cut(s) 2759
BstV1I GCAGC 6 cut(s) 394, 565, 1387, 1576, 1850, 2675
BstV2I GAAGAC 3 cut(s) 1699, 2103, 2454
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BstZ17I GTATAC 1 cut(s) 2104
BsuI GTATCC 1 cut(s) 2782
BsuRI GGCC 5 cut(s) 1546, 1781, 1855, 2130, 2300
BtgI CCRYGG 4 cut(s) 582, 1514, 1699, 2724
BtrI CACGTC 1 cut(s) 2062
BtsCI GGATG 7 cut(s) 27, 46, 193, 309, 891, 966, 1283
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 4 cut(s) 28, 156, 264, 1464
BveI ACCTGC 2 cut(s) 724, 2544
Cac8I GCNNGC 4 cut(s) 363, 603, 1924, 2156
CciI TCATGA 1 cut(s) 124
CfoI GCGC 1 cut(s) 2761
Cfr10I RCCGGY 1 cut(s) 2453
Cfr13I GGNCC 6 cut(s) 650, 860, 1518, 1854, 2398, 2730
CseI GACGC 1 cut(s) 2765
CsiI ACCWGGT 1 cut(s) 2698
Csp6I GTAC 6 cut(s) 144, 525, 559, 1647, 1902, 2683
CspAI ACCGGT 1 cut(s) 2453
CspCI CAANNNNNGTGG 2 cut(s) 1611, 1646
CviQI GTAC 6 cut(s) 144, 525, 559, 1647, 1902, 2683
DdeI CTNAG 8 cut(s) 148, 393, 763, 894, 1935, 2018, 2621, 2734
DraIII CACNNNGTG 1 cut(s) 1107
EaeI YGGCCR 1 cut(s) 2128
Eam1104I CTCTTC 4 cut(s) 318, 416, 669, 1696
EarI CTCTTC 4 cut(s) 318, 416, 669, 1696
Eco130I CCWWGG 5 cut(s) 207, 582, 1699, 2401, 2461
Eco24I GRGCYC 1 cut(s) 2779
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 2 cut(s) 1046, 1246
Eco47I GGWCC 5 cut(s) 650, 860, 1518, 2398, 2730
Eco57I CTGAAG 1 cut(s) 1627
Eco88I CYCGRG 1 cut(s) 786
Eco91I GGTNACC 1 cut(s) 989
EcoO109I RGGNCCY 2 cut(s) 860, 2730
EcoO65I GGTNACC 1 cut(s) 989
EcoRII CCWGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
EcoRV GATATC 2 cut(s) 1046, 1246
EcoT14I CCWWGG 5 cut(s) 207, 582, 1699, 2401, 2461
EcoT22I ATGCAT 2 cut(s) 633, 2112
EcoT38I GRGCYC 1 cut(s) 2779
ErhI CCWWGG 5 cut(s) 207, 582, 1699, 2401, 2461
Esp3I CGTCTC 1 cut(s) 1218
FalI AAGNNNNNCTT 6 cut(s) 209, 241, 1197, 1229, 2697, 2729
FaqI GGGAC 1 cut(s) 918
FauNDI CATATG 1 cut(s) 1173
FbaI TGATCA 3 cut(s) 832, 1096, 2491
FblI GTMKAC 2 cut(s) 389, 2103
Fnu4HI GCNGC 9 cut(s) 405, 408, 565, 579, 1401, 1590, 1864, 2028, 2664
FokI GGATG 7 cut(s) 14, 53, 200, 316, 898, 953, 1270
FriOI GRGCYC 1 cut(s) 2779
Fsp4HI GCNGC 9 cut(s) 405, 408, 565, 579, 1401, 1590, 1864, 2028, 2664
FspBI CTAG 6 cut(s) 828, 1104, 1157, 1928, 2145, 2435
GlaI GCGC 1 cut(s) 2760
GluI GCNGC 9 cut(s) 405, 408, 565, 579, 1401, 1590, 1864, 2028, 2664
HaeIII GGCC 5 cut(s) 1546, 1781, 1855, 2130, 2300
HapII CCGG 2 cut(s) 398, 2454
HgaI GACGC 1 cut(s) 2765
HhaI GCGC 1 cut(s) 2761
Hin6I GCGC 1 cut(s) 2759
HinP1I GCGC 1 cut(s) 2759
HincII GTYRAC 4 cut(s) 683, 1030, 1162, 1409
HindII GTYRAC 4 cut(s) 683, 1030, 1162, 1409
HpaI GTTAAC 1 cut(s) 1162
HpaII CCGG 2 cut(s) 398, 2454
Hpy188I TCNGA 6 cut(s) 318, 1444, 2009, 2257, 2276, 2421
Hpy99I CGWCG 1 cut(s) 2759
HpyCH4III ACNGT 6 cut(s) 94, 434, 1518, 1906, 1946, 2005
HpyCH4IV ACGT 5 cut(s) 1227, 1629, 2061, 2100, 2479
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 413, 628, 1808, 1988, 2765, 2774
HpyF3I CTNAG 8 cut(s) 148, 393, 763, 894, 1935, 2018, 2621, 2734
HpySE526I ACGT 5 cut(s) 1227, 1629, 2061, 2100, 2479
HspAI GCGC 1 cut(s) 2759
KpnI GGTACC 3 cut(s) 147, 1905, 2686
Ksp22I TGATCA 3 cut(s) 832, 1096, 2491
KspAI GTTAAC 1 cut(s) 1162
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 6 cut(s) 394, 565, 1387, 1576, 1850, 2675
LweI GCATC 6 cut(s) 31, 187, 985, 2198, 2483, 2650
MabI ACCWGGT 1 cut(s) 2698
MaeI CTAG 6 cut(s) 828, 1104, 1157, 1928, 2145, 2435
MaeII ACGT 5 cut(s) 1227, 1629, 2061, 2100, 2479
MaeIII GTNAC 3 cut(s) 151, 342, 989
MbiI CCGCTC 1 cut(s) 2030
MfeI CAATTG 2 cut(s) 535, 2054
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 6 cut(s) 549, 624, 1072, 1112, 1836, 2779
MlyI GAGTC 7 cut(s) 172, 900, 978, 1599, 1713, 1771, 2019
MmeI TCCRAC 2 cut(s) 628, 1287
Mph1103I ATGCAT 2 cut(s) 633, 2112
MseI TTAA 8 cut(s) 701, 1161, 1448, 2180, 2208, 2678, 2688, 2741
MslI CAYNNNNRTG 4 cut(s) 129, 1172, 2197, 2658
MspA1I CMGCKG 1 cut(s) 1400
MspCI CTTAAG 1 cut(s) 2677
MspI CCGG 2 cut(s) 398, 2454
MspR9I CCNGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
MunI CAATTG 2 cut(s) 535, 2054
Mva1269I GAATGC 1 cut(s) 773
MvaI CCWGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
MvnI CGCG 1 cut(s) 2759
MwoI GCNNNNNNNGC 7 cut(s) 64, 413, 628, 1808, 1988, 2765, 2774
NcoI CCATGG 2 cut(s) 582, 1699
NdeI CATATG 1 cut(s) 1173
NlaIV GGNNCC 9 cut(s) 145, 309, 652, 1069, 1833, 1903, 2467, 2684, 2731
NmuCI GTSAC 3 cut(s) 151, 342, 989
NsiI ATGCAT 2 cut(s) 633, 2112
NspI RCATGY 3 cut(s) 55, 1028, 2110
NspV TTCGAA 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 786
PagI TCATGA 1 cut(s) 124
PciI ACATGT 1 cut(s) 1024
PctI GAATGC 1 cut(s) 773
PfeI GAWTC 5 cut(s) 283, 1361, 1795, 2440, 2710
PflFI GACNNNGTC 1 cut(s) 987
PinAI ACCGGT 1 cut(s) 2453
PkrI GCNGC 9 cut(s) 406, 409, 566, 580, 1402, 1591, 1865, 2029, 2665
Ple19I CGATCG 1 cut(s) 870
PleI GAGTC 7 cut(s) 172, 900, 978, 1599, 1712, 1770, 2018
PpsI GAGTC 7 cut(s) 172, 900, 978, 1599, 1712, 1770, 2018
PpuMI RGGWCCY 2 cut(s) 860, 2730
PscI ACATGT 1 cut(s) 1024
PshAI GACNNNNGTC 1 cut(s) 2003
Psp5II RGGWCCY 2 cut(s) 860, 2730
Psp6I CCWGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
PspEI GGTNACC 1 cut(s) 989
PspGI CCWGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
PspN4I GGNNCC 9 cut(s) 145, 309, 652, 1069, 1833, 1903, 2467, 2684, 2731
PspPI GGNCC 6 cut(s) 650, 860, 1518, 1854, 2398, 2730
PspPPI RGGWCCY 2 cut(s) 860, 2730
PsrI GAACNNNNNNTAC 2 cut(s) 317, 349
PsuI RGATCY 1 cut(s) 307
PsyI GACNNNGTC 1 cut(s) 987
PvuI CGATCG 1 cut(s) 870
RsaI GTAC 6 cut(s) 145, 526, 560, 1648, 1903, 2684
RsaNI GTAC 6 cut(s) 144, 525, 559, 1647, 1902, 2683
RseI CAYNNNNRTG 4 cut(s) 129, 1172, 2197, 2658
SaqAI TTAA 8 cut(s) 701, 1161, 1448, 2180, 2208, 2678, 2688, 2741
SatI GCNGC 9 cut(s) 405, 408, 565, 579, 1401, 1590, 1864, 2028, 2664
Sau96I GGNCC 6 cut(s) 650, 860, 1518, 1854, 2398, 2730
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 7 cut(s) 172, 900, 978, 1599, 1713, 1771, 2019
ScrFI CCNGG 5 cut(s) 1543, 2121, 2330, 2644, 2700
SduI GDGCHC 6 cut(s) 549, 624, 1072, 1112, 1836, 2779
SexAI ACCWGGT 1 cut(s) 2698
SfaNI GCATC 6 cut(s) 31, 187, 985, 2198, 2483, 2650
SfcI CTRYAG 1 cut(s) 1942
Sfr274I CTCGAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 286
SinI GGWCC 5 cut(s) 650, 860, 1518, 2398, 2730
SlaI CTCGAG 1 cut(s) 786
SmiMI CAYNNNNRTG 4 cut(s) 129, 1172, 2197, 2658
SmlI CTYRAG 5 cut(s) 173, 518, 786, 2225, 2677
SmoI CTYRAG 5 cut(s) 173, 518, 786, 2225, 2677
SsiI CCGC 7 cut(s) 141, 404, 565, 1304, 1398, 1650, 2028
SspI AATATT 2 cut(s) 295, 1076
SspMI CTAG 6 cut(s) 828, 1104, 1157, 1928, 2145, 2435
StyD4I CCNGG 5 cut(s) 1541, 2119, 2328, 2642, 2698
StyI CCWWGG 5 cut(s) 207, 582, 1699, 2401, 2461
TaaI ACNGT 6 cut(s) 94, 434, 1518, 1906, 1946, 2005
TaiI ACGT 5 cut(s) 1230, 1632, 2064, 2103, 2482
TaqI TCGA 4 cut(s) 286, 349, 787, 1320
TaqII GACCGA 1 cut(s) 977
TatI WGTACW 2 cut(s) 524, 558
TauI GCSGC 3 cut(s) 407, 567, 2030
TfiI GAWTC 5 cut(s) 283, 1361, 1795, 2440, 2710
Tru1I TTAA 8 cut(s) 701, 1161, 1448, 2180, 2208, 2678, 2688, 2741
Tru9I TTAA 8 cut(s) 701, 1161, 1448, 2180, 2208, 2678, 2688, 2741
TscAI CASTG 4 cut(s) 28, 156, 271, 1471
TseFI GTSAC 3 cut(s) 151, 342, 989
TseI GCWGC 6 cut(s) 407, 578, 1400, 1589, 1863, 2663
Tsp45I GTSAC 3 cut(s) 151, 342, 989
TspRI CASTG 4 cut(s) 28, 156, 271, 1471
Tth111I GACNNNGTC 1 cut(s) 987
Vha464I CTTAAG 1 cut(s) 2677
VneI GTGCAC 1 cut(s) 1108
VpaK11BI GGWCC 5 cut(s) 650, 860, 1518, 2398, 2730
XapI RAATTY 4 cut(s) 12, 248, 1276, 2291
XceI RCATGY 3 cut(s) 55, 1028, 2110
XcmI CCANNNNNNNNNTGG 1 cut(s) 969
XhoI CTCGAG 1 cut(s) 786
XmiI GTMKAC 2 cut(s) 389, 2103
XspI CTAG 6 cut(s) 828, 1104, 1157, 1928, 2145, 2435
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 2 cut(s) 633, 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.