RchiOBHm_Chr5g0078371

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000071
Physical Location & Seq
Reverse (-)
84265892 .. 84269735
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35290

Sequence Viewer

Length: 3183 bp
ATGAATTCCTCAATGATGCAATCAGAGAAACAGACTATTGGGCATTCGGTGACTACCGAATTTTCTGTTATAGCTCAACGCAAACAGGGTCATCCTCCAGGCTTCTCAGGACCTTCTCCACGCCGCCTTCTGGGTAAACCAAATCCATATGCAAACAAAAGGTGCATTATCTGTGGGGAATTAGGTCATAGCAAAGAGCGGTGCTATGAAGTAATTGGTTACCCTGATTGGTGGGACTTCACCAAGAAACCGCGAAAGAATCTGGGCAAGGCCGCTGTTGCTACTATAGAGGAAGTTTACCTAGACAATGCCTCTGCTAATGTAGCGCAGTCAGGTTTGAAGGGTAAGGTTACTTTTAATAGTATATGGATAATTGATACAGGTGCATCTGACCATATGACCAATGACCCAAGTCTTGTGAAAAACCTTAGACGTTCCCCTCAAGACGTTGTCTCTACTGCTGATGGTACTCCAACTCCGGTCACCGGAGAAGGTTCTATTGCTTTATCTGATACCTTAACCCTTGAATCTGTCCTAGTTGTTCCATCACTAGCCTATAATCTCCTGTCTGTTGGTCAAATTATTTTAGCTCTTGCATGTATTGTGACCTTCTATCCGTCTTTCTGTGTGTTTCAGAACATTCTGACTCGACGGATTCTTGGTTATGGTGTTAGAAGGGGGAAATTATACTATCTGGATCTGACAGAGACTGGAAAGAAACAGAAGCATCTTTTGGGACAAGCTAATCAGATCAACGGGGTAGAGAATGCGAAGGAAGCTGTATGGTTATGGCATCGCCGTTTAGGTCATCTATCCTTTCGTTATCTTAAGAAGCTGCAACCTCATTTGTTTTCAGTTGTTAGTGATTTGGATTTCCGCTGTGACATTTGTGAACTGGCCAAGAGTCACCGTATTTCATATTCACCAAGTCTTAATAAAAGTCCTGTTCCTTTTATGAAGATTCACTCTGATGTCTGGGGTCCTGCGAAAATTCCTTCTCTTTCTGGAGCTCGGTATTTTGTAACGTTTATTGATGATTGCACTCGCATGACATGGGTGGGCTGGGGAGCTTCTCAGTCTTCCCTTCAGAGGGAGAGAGGTTGTGAAGGGAATCCTCGTTCTATTATTGATTTTGATGTCTTTGAAGACTTGGAAAATTTGGAGGAACGATTTGAAGGTAGAAATTCGGAAACAGAAAATGCAGTTTCCGAAGAGAGCATTGTGAATTCCGAAACAGAAAATGCAACTGCTGAACAGAGTGCAGTTGTCGAACAAAGCATTATGAATTCCGAAACAGAAAATGCGACTGCTGAACAAAGTGTGGTGAATTCTGAAACAAAAAATGCAACTGCTGAACAGAGCGTTGTGAATTCCGAGACAGAAGAGACGACTTTTCTGGATAGTTTGAATCAAAATCAAGACGTATCTGAAGCTTACACACAAGATATTCCCCCTTCTGCATCACCAACTGAAGATCCATGTCAGAATTGTCCACCTCAGGTACCCCTAAACTTTAATGAGTCTTCTGGGTTAGAAAGTGTCGAACCTAGGAAATCACAAAGGGTTACCAAGGGAATTCCTAAGAAACAATATGAACCAGATATCAAAGCCAAAGCTAAATACCCTATAGCTAATTTTATGTCTAACCATAGGATTTCTGGGTCACATGCACTTGTTGTTGATCAATTATCTATTGTATCTATTCCTAGTAACGTGCAGGATGCATTGACGGATCCAAAATGGACAAAGGCGATGAATGAAGAAATGGAAGCTCTTCAAAAGAATGCAACATGGGAGCTAGTACCTATGCCGGTTGGAAAGAAGACTGTAGGATGTCGTTGGGTATTTACTGTGAAGCTTAATGCAGATGGAACTATTAATAGATACAAGGCGAGGTTGGTTGCCAAAGGATATACACAACGCTATGGGATTGATTATGAGGAGACTTTTGCACCTGTGGCAAAGATTAATACTGTTCGGATTCTAATCTCACTTGCAGCAAACAAAGATTGGCCCTTGCATCAGTTTGATGTGAAGAATGCGTTCCTTAATGGGAATTTGGAGGAAGAAGTGTACATGGATGTGCCCCCAGGTGTTAAGAATTACCCAAGTGACGTTGGCAAGGTGTGTAAATTGAAGAAGTCTTTGTATGGCCTGAAGCAGTCGCCAAGAGCTTGGTTTGGAAGATTTTCAAAGTCCATGAAAGCCTTTGGATACAGACAGAGCAATTCTGACCATACCTTGTTTATCAAACGCAAGAATGGTAAGATTACAGCTCTTATTGTGTATGTTGATGACATGATTGTTACAGGGGATGATCCGAAAGAGATGAATGAATTGCAAAAGTATATGTCAAAGGAGTTTGAAATGAAGGATCTAGGACAACTGAAGTATTTTCTGGGTATTGAAGTTGCAAGGTCTAAGAAGGGGATTTTGCTTTCACAAAGGAAGTATGTACTTGATTTACTTGCTGAAAAGGGGATGCTGGACTGCAGACCAATGGAGACACCCATTGAGATGAGTCACAGACTTGCTATTTATCCTGATCAAGTTCCAACTGATAAAGGGAGGTATCAACGTCTTGTAGGAAGGTTGATTTATCTTTCACATACTAGACCTGATATTGCTTATGCTGTGAGTGTTGTTAGTCAATTTATGCATTGTCCTAGTGAAGAGCATATGGATGTAGTCTTTCGTATTTTGAGGTACTTGAAGATGGCGCCAGATTGGGCTGGTGATAAAACTGACAGACGTTCTACATCTGGGTACTTCACTTTTGTTGGAGGGAACCTTGTCACTTGGCGTAGCAAAAAGCAGAAAGTTGTTGCCAGATCAAGTGCAGAAGCTGAGTTCCGAGGTATGGCACACGGAGTCTGTGAAATGTTGTGGATTCGTAATGTCTTGAAAGACCTGGGTTACAAGCTTAAAAAGCCTATGGATTTGCATTGTGATAATACAGCTGCCATTGAGATTGCACATAATCCAGTTCAGCATGATAGAACAAAGCATGTGGAGGTTGACCGTCATTTTATTAAAGAAAATCTTGACAGAAAGGTTATTCGCTTTCCATTTGTAAACTCAGAGGAGCAATTAGCTGATGTTCTTACTAAAGGAGTGTCCAGGAAGGTATTTGACAGCTCAGTTGACAAGTTGGGCATGATAGACATCTATGCACCAACTTGA

Protein Analysis

1060

Amino Acids

119.4

Weight (kDa)

8.47

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 123 - 194 2.7e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 229 - 302 4.9e-15 GAG-pre-integrase domain
RVT_2 PF07727 595 - 839 5.9e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1501
AccB1I GGYRCC 2 cut(s) 1501, 2719
AccBSI CCGCTC 1 cut(s) 199
AccII CGCG 1 cut(s) 253
AciI CCGC 5 cut(s) 124, 199, 251, 273, 877
AclI AACGTT 1 cut(s) 1025
AclWI GGATC 6 cut(s) 705, 1469, 1727, 1740, 2312, 2382
AcoI YGGCCR 1 cut(s) 897
AcuI CTGAAG 5 cut(s) 1070, 1449, 1491, 2177, 2408
AcyI GRCGYC 1 cut(s) 2720
AfaI GTAC 7 cut(s) 469, 1503, 1803, 2075, 2457, 2708, 2768
AfiI CCNNNNNNNGG 5 cut(s) 130, 485, 1089, 1090, 2860
AflII CTTAAG 1 cut(s) 827
AjnI CCWGG 4 cut(s) 97, 2089, 2910, 3119
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AloI GAACNNNNNNTCC 2 cut(s) 1102, 1134
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 6 cut(s) 457, 701, 1370, 1379, 1937, 2498
AlwI GGATC 6 cut(s) 705, 1469, 1727, 1740, 2312, 2382
AlwNI CAGNNNCTG 2 cut(s) 710, 2846
AoxI GGCC 4 cut(s) 270, 897, 2012, 2152
ApeKI GCWGC 3 cut(s) 835, 1997, 2960
AseI ATTAAT 2 cut(s) 1878, 1968
Asp700I GAANNNNTTC 3 cut(s) 1773, 2042, 2188
Asp718I GGTACC 1 cut(s) 1501
AspA2I CCTAGG 1 cut(s) 1547
AspLEI GCGC 2 cut(s) 328, 2722
AspS9I GGNCC 3 cut(s) 110, 980, 2013
AsuHPI GGTGA 8 cut(s) 61, 232, 475, 899, 915, 1336, 1455, 2747
AvaII GGWCC 2 cut(s) 110, 980
AvrII CCTAGG 1 cut(s) 1547
AxyI CCTNAGG 1 cut(s) 1497
BaeGI GKGCMC 1 cut(s) 2088
BalI TGGCCA 1 cut(s) 899
BamHI GGATCC 1 cut(s) 1732
BanI GGYRCC 2 cut(s) 1501, 2719
BanII GRGCYC 1 cut(s) 1012
BbsI GAAGAC 4 cut(s) 1071, 1152, 1515, 1829
Bbv12I GWGCWC 1 cut(s) 1012
BbvI GCAGC 3 cut(s) 822, 2009, 2947
BccI CCATC 4 cut(s) 458, 553, 1862, 2710
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 4 cut(s) 99, 2091, 2912, 3121
BciVI GTATCC 1 cut(s) 2207
BclI TGATCA 2 cut(s) 1681, 2545
BcoDI GTCTC 6 cut(s) 457, 701, 1370, 1379, 1937, 2498
BfaI CTAG 9 cut(s) 302, 536, 551, 1548, 1707, 1799, 2378, 2613, 2667
BfmI CTRYAG 4 cut(s) 285, 1626, 1827, 2491
BfoI RGCGCY 1 cut(s) 2723
BfrI CTTAAG 1 cut(s) 827
BfuI GTATCC 1 cut(s) 2207
BisI GCNGC 5 cut(s) 124, 273, 836, 1998, 2961
BlnI CCTAGG 1 cut(s) 1547
BlsI GCNGC 5 cut(s) 125, 274, 837, 1999, 2962
Bme1390I CCNGG 4 cut(s) 99, 2091, 2912, 3121
Bme18I GGWCC 2 cut(s) 110, 980
BmgT120I GGNCC 3 cut(s) 110, 980, 2013
BmiI GGNNCC 5 cut(s) 981, 1503, 1734, 2721, 2789
BmrFI CCNGG 4 cut(s) 99, 2091, 2912, 3121
BmsI GCATC 8 cut(s) 6, 395, 736, 802, 1469, 1711, 2029, 2472
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 4 cut(s) 1071, 1152, 1515, 1829
BpmI CTGGAG 2 cut(s) 81, 1026
BpuEI CTTGAG 1 cut(s) 426
BsaBI GATNNNNATC 2 cut(s) 1985, 3164
BsaHI GRCGYC 1 cut(s) 2720
BsaJI CCNNGG 5 cut(s) 1547, 1569, 2089, 2854, 2911
BsaWI WCCGGW 2 cut(s) 478, 485
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 5 cut(s) 130, 485, 1089, 1090, 2860
Bse118I RCCGGY 1 cut(s) 1810
Bse1I ACTGG 3 cut(s) 715, 900, 2984
Bse21I CCTNAGG 1 cut(s) 1497
Bse8I GATNNNNATC 2 cut(s) 1985, 3164
BseBI CCWGG 4 cut(s) 99, 2091, 2912, 3121
BseDI CCNNGG 5 cut(s) 1547, 1569, 2089, 2854, 2911
BseGI GGATG 7 cut(s) 91, 1726, 1838, 2086, 2320, 2487, 2689
BseJI GATNNNNATC 2 cut(s) 1985, 3164
BseLI CCNNNNNNNGG 5 cut(s) 130, 485, 1089, 1090, 2860
BseMII CTCAG 6 cut(s) 120, 1088, 1511, 2838, 3093, 3153
BseNI ACTGG 3 cut(s) 715, 900, 2984
BseRI GAGGAG 2 cut(s) 1955, 3098
BseSI GKGCMC 1 cut(s) 2088
BseXI GCAGC 3 cut(s) 822, 2009, 2947
BseYI CCCAGC 1 cut(s) 1062
BsgI GTGCAG 3 cut(s) 1281, 1736, 2859
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 4 cut(s) 272, 899, 2014, 2154
BshNI GGYRCC 2 cut(s) 1501, 2719
BsiHKAI GWGCWC 1 cut(s) 1012
BsiSI CCGG 3 cut(s) 479, 486, 1811
BslFI GGGAC 2 cut(s) 248, 750
BslI CCNNNNNNNGG 5 cut(s) 130, 485, 1089, 1090, 2860
BsmAI GTCTC 6 cut(s) 457, 701, 1370, 1379, 1937, 2498
BsmBI CGTCTC 1 cut(s) 1379
BsmFI GGGAC 2 cut(s) 248, 750
BsmI GAATGC 4 cut(s) 43, 772, 1789, 2044
BsnI GGCC 4 cut(s) 272, 899, 2014, 2154
Bsp1286I GDGCHC 2 cut(s) 1012, 2088
Bsp1407I TGTACA 1 cut(s) 2073
Bsp143I GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
BspACI CCGC 5 cut(s) 124, 199, 251, 273, 877
BspANI GGCC 4 cut(s) 272, 899, 2014, 2154
BspCNI CTCAG 6 cut(s) 119, 1087, 1510, 2839, 3092, 3152
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 5 cut(s) 981, 1503, 1734, 2721, 2789
BspMAI CTGCAG 1 cut(s) 2495
BspPI GGATC 6 cut(s) 705, 1469, 1727, 1740, 2312, 2382
BspQI GCTCTTC 2 cut(s) 1779, 2667
BspT107I GGYRCC 2 cut(s) 1501, 2719
BspTI CTTAAG 1 cut(s) 827
BsrBI CCGCTC 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 1810
BsrGI TGTACA 1 cut(s) 2073
BsrI ACTGG 3 cut(s) 715, 900, 2984
BssAI RCCGGY 1 cut(s) 1810
BssECI CCNNGG 5 cut(s) 1547, 1569, 2089, 2854, 2911
BssMI GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
BssNI GRCGYC 1 cut(s) 2720
BssT1I CCWWGG 2 cut(s) 1547, 1569
Bst2UI CCWGG 4 cut(s) 99, 2091, 2912, 3121
Bst4CI ACNGT 5 cut(s) 911, 1828, 1852, 1975, 3023
Bst6I CTCTTC 4 cut(s) 1206, 1377, 1779, 2667
BstACI GRCGYC 1 cut(s) 2720
BstAFI CTTAAG 1 cut(s) 827
BstAUI TGTACA 1 cut(s) 2073
BstDEI CTNAG 9 cut(s) 106, 428, 1074, 1497, 1581, 2421, 2847, 3079, 3139
BstEII GGTNACC 3 cut(s) 218, 481, 1564
BstF5I GGATG 7 cut(s) 91, 1726, 1838, 2086, 2320, 2487, 2689
BstFNI CGCG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 2723
BstHHI GCGC 2 cut(s) 328, 2722
BstKTI GATC 9 cut(s) 700, 753, 1477, 1684, 1735, 2320, 2377, 2548, 2834
BstMAI GTCTC 6 cut(s) 457, 701, 1370, 1379, 1937, 2498
BstMBI GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
BstMWI GCNNNNNNNGC 5 cut(s) 278, 323, 776, 1958, 2929
BstNI CCWGG 4 cut(s) 99, 2091, 2912, 3121
BstNSI RCATGY 3 cut(s) 600, 1670, 3011
BstPAI GACNNNNGTC 1 cut(s) 411
BstPI GGTNACC 3 cut(s) 218, 481, 1564
BstSCI CCNGG 4 cut(s) 97, 2089, 2910, 3119
BstSFI CTRYAG 4 cut(s) 285, 1626, 1827, 2491
BstSLI GKGCMC 1 cut(s) 2088
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 3 cut(s) 822, 2009, 2947
BstV2I GAAGAC 4 cut(s) 1071, 1152, 1515, 1829
BstX2I RGATCY 4 cut(s) 697, 1474, 1732, 2374
BstXI CCANNNNNNTGG 1 cut(s) 2175
BstYI RGATCY 4 cut(s) 697, 1474, 1732, 2374
Bsu36I CCTNAGG 1 cut(s) 1497
BsuI GTATCC 1 cut(s) 2207
BsuRI GGCC 4 cut(s) 272, 899, 2014, 2154
BtgZI GCGATG 2 cut(s) 779, 1766
BtsCI GGATG 7 cut(s) 91, 1726, 1838, 2086, 2320, 2487, 2689
CaiI CAGNNNCTG 2 cut(s) 710, 2846
CfoI GCGC 2 cut(s) 328, 2722
Cfr10I RCCGGY 1 cut(s) 1810
Cfr13I GGNCC 3 cut(s) 110, 980, 2013
Csp6I GTAC 7 cut(s) 468, 1502, 1802, 2074, 2456, 2707, 2767
CspCI CAANNNNNGTGG 2 cut(s) 2991, 3026
CviQI GTAC 7 cut(s) 468, 1502, 1802, 2074, 2456, 2707, 2767
DdeI CTNAG 9 cut(s) 106, 428, 1074, 1497, 1581, 2421, 2847, 3079, 3139
DinI GGCGCC 1 cut(s) 2721
DpnI GATC 9 cut(s) 699, 752, 1476, 1683, 1734, 2319, 2376, 2547, 2833
DpnII GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 4 cut(s) 1206, 1377, 1779, 2667
EarI CTCTTC 4 cut(s) 1206, 1377, 1779, 2667
Ecl136II GAGCTC 1 cut(s) 1010
Eco130I CCWWGG 2 cut(s) 1547, 1569
Eco24I GRGCYC 1 cut(s) 1012
Eco32I GATATC 1 cut(s) 1603
Eco47I GGWCC 2 cut(s) 110, 980
Eco53kI GAGCTC 1 cut(s) 1010
Eco57I CTGAAG 5 cut(s) 1070, 1449, 1491, 2177, 2408
Eco81I CCTNAGG 1 cut(s) 1497
Eco91I GGTNACC 3 cut(s) 218, 481, 1564
EcoICRI GAGCTC 1 cut(s) 1010
EcoO109I RGGNCCY 2 cut(s) 110, 980
EcoO65I GGTNACC 3 cut(s) 218, 481, 1564
EcoRI GAATTC 6 cut(s) 4, 1225, 1285, 1327, 1369, 1575
EcoRII CCWGG 4 cut(s) 97, 2089, 2910, 3119
EcoRV GATATC 1 cut(s) 1603
EcoT14I CCWWGG 2 cut(s) 1547, 1569
EcoT22I ATGCAT 2 cut(s) 1726, 2661
EcoT38I GRGCYC 1 cut(s) 1012
EgeI GGCGCC 1 cut(s) 2721
EheI GGCGCC 1 cut(s) 2721
ErhI CCWWGG 2 cut(s) 1547, 1569
Esp3I CGTCTC 1 cut(s) 1379
FalI AAGNNNNNCTT 2 cut(s) 3027, 3059
FaqI GGGAC 2 cut(s) 248, 750
FauNDI CATATG 3 cut(s) 148, 396, 2679
FbaI TGATCA 2 cut(s) 1681, 2545
Fnu4HI GCNGC 5 cut(s) 124, 273, 836, 1998, 2961
FokI GGATG 7 cut(s) 78, 1733, 1845, 2093, 2327, 2494, 2696
FriOI GRGCYC 1 cut(s) 1012
Fsp4HI GCNGC 5 cut(s) 124, 273, 836, 1998, 2961
FspBI CTAG 9 cut(s) 302, 536, 551, 1548, 1707, 1799, 2378, 2613, 2667
GlaI GCGC 2 cut(s) 327, 2721
GluI GCNGC 5 cut(s) 124, 273, 836, 1998, 2961
GsaI CCCAGC 1 cut(s) 1066
GsuI CTGGAG 2 cut(s) 81, 1026
HaeII RGCGCY 1 cut(s) 2723
HaeIII GGCC 4 cut(s) 272, 899, 2014, 2154
HapII CCGG 3 cut(s) 479, 486, 1811
HhaI GCGC 2 cut(s) 328, 2722
Hin1I GRCGYC 1 cut(s) 2720
Hin6I GCGC 2 cut(s) 326, 2720
HinP1I GCGC 2 cut(s) 326, 2720
HincII GTYRAC 2 cut(s) 3019, 3145
HindII GTYRAC 2 cut(s) 3019, 3145
HindIII AAGCTT 3 cut(s) 1431, 1856, 2921
HpaII CCGG 3 cut(s) 479, 486, 1811
HphI GGTGA 8 cut(s) 61, 232, 475, 899, 915, 1336, 1455, 2747
Hpy166II GTNNAC 8 cut(s) 137, 298, 893, 1493, 2074, 3019, 3076, 3145
Hpy188III TCNNGA 9 cut(s) 108, 443, 695, 1005, 1397, 1418, 2543, 2902, 3044
Hpy8I GTNNAC 8 cut(s) 137, 298, 893, 1493, 2074, 3019, 3076, 3145
Hpy99I CGWCG 1 cut(s) 654
HpyCH4III ACNGT 5 cut(s) 911, 1828, 1852, 1975, 3023
HpyCH4IV ACGT 8 cut(s) 433, 447, 1025, 1422, 1713, 2115, 2578, 2752
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 323, 776, 1958, 2929
HpyF3I CTNAG 9 cut(s) 106, 428, 1074, 1497, 1581, 2421, 2847, 3079, 3139
HpySE526I ACGT 8 cut(s) 433, 447, 1025, 1422, 1713, 2115, 2578, 2752
Hsp92I GRCGYC 1 cut(s) 2720
HspAI GCGC 2 cut(s) 326, 2720
KasI GGCGCC 1 cut(s) 2719
KpnI GGTACC 1 cut(s) 1505
Ksp22I TGATCA 2 cut(s) 1681, 2545
Kzo9I GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
LguI GCTCTTC 2 cut(s) 1779, 2667
LmnI GCTCC 4 cut(s) 1007, 1067, 1795, 3085
Lsp1109I GCAGC 3 cut(s) 822, 2009, 2947
LweI GCATC 8 cut(s) 6, 395, 736, 802, 1469, 1711, 2029, 2472
MaeI CTAG 9 cut(s) 302, 536, 551, 1548, 1707, 1799, 2378, 2613, 2667
MaeII ACGT 8 cut(s) 433, 447, 1025, 1422, 1713, 2115, 2578, 2752
MalI GATC 9 cut(s) 699, 752, 1476, 1683, 1734, 2319, 2376, 2547, 2833
MbiI CCGCTC 1 cut(s) 199
MboI GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
MflI RGATCY 4 cut(s) 697, 1474, 1732, 2374
MhlI GDGCHC 2 cut(s) 1012, 2088
MlsI TGGCCA 1 cut(s) 899
MluNI TGGCCA 1 cut(s) 899
Mly113I GGCGCC 1 cut(s) 2720
MlyI GAGTC 5 cut(s) 640, 913, 1529, 2530, 2880
MmeI TCCRAC 4 cut(s) 497, 1795, 2579, 2761
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 2 cut(s) 1726, 2661
MroXI GAANNNNTTC 3 cut(s) 1773, 2042, 2188
MscI TGGCCA 1 cut(s) 899
MslI CAYNNNNRTG 5 cut(s) 969, 1046, 2081, 2516, 2682
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 3 cut(s) 275, 879, 2960
MspCI CTTAAG 1 cut(s) 827
MspI CCGG 3 cut(s) 479, 486, 1811
MspR9I CCNGG 4 cut(s) 99, 2091, 2912, 3121
Mva1269I GAATGC 4 cut(s) 43, 772, 1789, 2044
MvaI CCWGG 4 cut(s) 99, 2091, 2912, 3121
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 5 cut(s) 278, 323, 776, 1958, 2929
NarI GGCGCC 1 cut(s) 2720
NdeI CATATG 3 cut(s) 148, 396, 2679
NdeII GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
NlaIV GGNNCC 5 cut(s) 981, 1503, 1734, 2721, 2789
NmuCI GTSAC 9 cut(s) 49, 481, 604, 881, 905, 1662, 2111, 2522, 2794
NsiI ATGCAT 2 cut(s) 1726, 2661
NspI RCATGY 3 cut(s) 600, 1670, 3011
PciSI GCTCTTC 2 cut(s) 1779, 2667
PctI GAATGC 4 cut(s) 43, 772, 1789, 2044
PdmI GAANNNNTTC 3 cut(s) 1773, 2042, 2188
PfeI GAWTC 8 cut(s) 259, 527, 655, 961, 1111, 1408, 1981, 2890
PflFI GACNNNGTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 3119
PkrI GCNGC 5 cut(s) 125, 274, 837, 1999, 2962
PleI GAGTC 5 cut(s) 640, 912, 1528, 2529, 2879
PluTI GGCGCC 1 cut(s) 2723
PpsI GAGTC 5 cut(s) 640, 912, 1528, 2529, 2879
PpuMI RGGWCCY 2 cut(s) 110, 980
PshAI GACNNNNGTC 1 cut(s) 411
PshBI ATTAAT 2 cut(s) 1878, 1968
Psp124BI GAGCTC 1 cut(s) 1012
Psp1406I AACGTT 1 cut(s) 1025
Psp5II RGGWCCY 2 cut(s) 110, 980
Psp6I CCWGG 4 cut(s) 97, 2089, 2910, 3119
PspEI GGTNACC 3 cut(s) 218, 481, 1564
PspFI CCCAGC 1 cut(s) 1062
PspGI CCWGG 4 cut(s) 97, 2089, 2910, 3119
PspN4I GGNNCC 5 cut(s) 981, 1503, 1734, 2721, 2789
PspPI GGNCC 3 cut(s) 110, 980, 2013
PspPPI RGGWCCY 2 cut(s) 110, 980
PstI CTGCAG 1 cut(s) 2495
PstNI CAGNNNCTG 2 cut(s) 710, 2846
PsuI RGATCY 4 cut(s) 697, 1474, 1732, 2374
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 2960
RsaI GTAC 7 cut(s) 469, 1503, 1803, 2075, 2457, 2708, 2768
RsaNI GTAC 7 cut(s) 468, 1502, 1802, 2074, 2456, 2707, 2767
RseI CAYNNNNRTG 5 cut(s) 969, 1046, 2081, 2516, 2682
SacI GAGCTC 1 cut(s) 1012
SapI GCTCTTC 2 cut(s) 1779, 2667
SatI GCNGC 5 cut(s) 124, 273, 836, 1998, 2961
Sau3AI GATC 9 cut(s) 697, 750, 1474, 1681, 1732, 2317, 2374, 2545, 2831
Sau96I GGNCC 3 cut(s) 110, 980, 2013
SchI GAGTC 5 cut(s) 640, 913, 1529, 2530, 2880
ScrFI CCNGG 4 cut(s) 99, 2091, 2912, 3121
SduI GDGCHC 2 cut(s) 1012, 2088
SfaNI GCATC 8 cut(s) 6, 395, 736, 802, 1469, 1711, 2029, 2472
SfcI CTRYAG 4 cut(s) 285, 1626, 1827, 2491
SfoI GGCGCC 1 cut(s) 2721
SinI GGWCC 2 cut(s) 110, 980
SmiMI CAYNNNNRTG 5 cut(s) 969, 1046, 2081, 2516, 2682
SmlI CTYRAG 2 cut(s) 441, 827
SmoI CTYRAG 2 cut(s) 441, 827
SsiI CCGC 5 cut(s) 124, 199, 251, 273, 877
SspDI GGCGCC 1 cut(s) 2719
SspMI CTAG 9 cut(s) 302, 536, 551, 1548, 1707, 1799, 2378, 2613, 2667
SstI GAGCTC 1 cut(s) 1012
StyD4I CCNGG 4 cut(s) 97, 2089, 2910, 3119
StyI CCWWGG 2 cut(s) 1547, 1569
TaaI ACNGT 5 cut(s) 911, 1828, 1852, 1975, 3023
TaiI ACGT 8 cut(s) 436, 450, 1028, 1425, 1716, 2118, 2581, 2755
TaqI TCGA 3 cut(s) 649, 1269, 1542
TatI WGTACW 2 cut(s) 2073, 2455
TauI GCSGC 2 cut(s) 126, 275
TfiI GAWTC 8 cut(s) 259, 527, 655, 961, 1111, 1408, 1981, 2890
TseFI GTSAC 9 cut(s) 49, 481, 604, 881, 905, 1662, 2111, 2522, 2794
TseI GCWGC 3 cut(s) 835, 1997, 2960
Tsp45I GTSAC 9 cut(s) 49, 481, 604, 881, 905, 1662, 2111, 2522, 2794
TspGWI ACGGA 4 cut(s) 606, 667, 1745, 2883
Tth111I GACNNNGTC 1 cut(s) 449
Vha464I CTTAAG 1 cut(s) 827
VpaK11BI GGWCC 2 cut(s) 110, 980
VspI ATTAAT 2 cut(s) 1878, 1968
XceI RCATGY 3 cut(s) 600, 1670, 3011
XcmI CCANNNNNNNNNTGG 1 cut(s) 1655
XmaJI CCTAGG 1 cut(s) 1547
XmnI GAANNNNTTC 3 cut(s) 1773, 2042, 2188
XspI CTAG 9 cut(s) 302, 536, 551, 1548, 1707, 1799, 2378, 2613, 2667
Zsp2I ATGCAT 2 cut(s) 1726, 2661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.