RchiOBHm_Chr3g0468181

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000071
Physical Location & Seq
Forward (+)
14249742 .. 14251205
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43410

Sequence Viewer

Length: 1464 bp
ATGATCAAACTCAGTCAGTCTTGCCAAATTCAACTGAAGACCCCCCTACTCCTAAGTCCTACCACATCGGTCCCTATTTCTCATGAGGATGCTCCTGAGGTAAGTCTCAATCCTAAACTTGAGTCAGATATTGTTATAAATGATGAACATGTCCTTGATCATGAGTCTAATGTTATCATAGATAATGAGGCTAACCCTGAAACAGACCCCAACACTGAAACAAGTGTGCCCCCCAAATATCAATTGCCACATCGTACAACGAGAGGGAAGCCTAAAGCCCAATATGAGCCAAATCTTAAGGTTAAAGCCAAATACCCCATAACTAATTATGTGTCAACTCATAGGTTGTCCGAGTCATATGCATCATTTGTGTTTCAATTATCTACTATTGTCATTGCAAATAAAGTGCAAGAAGCATTAGCAGATCCCAAATGGACTGAGGCAATGAAGGTTGAAATGGAAGCTTTGGAGAAGAATGATACATGGGAACAAGTACCAAAACCTCAAGGAAAGAAAACAGTGCAGTGCAGGTGGATATATACCTTGAAATATGCTGCTGATGGGACACTGAACAGGTACAAAGCAAGGTTAGCTGCTAAAGGCTACACACAAACATATGTTGTTGATTATCAGGAAACATTTGCACCGGTAGCCAAGATTACTACTATCCGTGTACTACTATCTCTAACTGCGAACTTGGATTGGCCCCTGAAGCAGTTTGATGTGAAGAATGCATTCTTGCATGGAGATCTGAAAGAGGAAGTATACATGTATCTACCTCCAGGGTTCATTGCACATTCGCAGGAAGGAATGGTATGCAGATTGAAGAAGTCTCTATATAGGCTCAAGCAATCTCCGAGAGCATGGTTTGGAAGGTTCAGACAGTCTCTGAAGGCATTTGGCTACAAGCAGAGTAACTCATATCACACTCTCATCTTGAAGCGTAGAAGGAATAAGATAACTGCCTTGATTATCTATGTGGATGATATGATAGTGACAGGAGATGATCAGGAAGAAGTAAAGAAGTTGGAAGCTTATCTATCATATGAATTTGAGATGAAAGACCTTGGTGGATTAAAGTACTTCCTAGGCATTGAGGTGGCCAGATCAAAGGAAGGCATTTTCCTATCTCAAAGGAAATATGTCTTAGACCTACTCACTGAAACAGGCATGTTGGCTTGCAAACCTGCAGATACACCCATGGAGCAAAATCACAAGTTAGCAGAATATCCTGATCAAGTGCCTACTAACCGAGAGAGATATCAAAGACTAGTTGGCAGACTTATCTATCTCCCTCACACTCGTCCTGACATTGCATATGCTGTGAGTGCGGTGAGCCAATTCATGCATGCTCCAAGTGAAGACCATATGAATGCAGTATATCGTATTCTTAGTTATTTGAAGTCTGCTCCAGGCAAAGGGATTATGTTTGCTAAAAATAATCATTTGGAGGTGTTATACTAA

Protein Analysis

487

Amino Acids

55.88

Weight (kDa)

8.91

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 159 - 402 1.2e-66 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
AarI CACCTGC 1 cut(s) 519
Acc36I ACCTGC 2 cut(s) 519, 1195
AccI GTMKAC 1 cut(s) 765
AciI CCGC 1 cut(s) 1331
AclWI GGATC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 1101
AcsI RAATTY 2 cut(s) 27, 1049
AcuI CTGAAG 3 cut(s) 56, 731, 911
AfaI GTAC 5 cut(s) 256, 495, 578, 675, 1082
AfiI CCNNNNNNNGG 1 cut(s) 1418
AflII CTTAAG 1 cut(s) 296
AflIII ACRYGT 2 cut(s) 148, 768
AgeI ACCGGT 1 cut(s) 646
AgsI TTSAA 7 cut(s) 32, 377, 455, 547, 826, 940, 1402
AhlI ACTAGT 1 cut(s) 1270
AjnI CCWGG 2 cut(s) 781, 1411
AluBI AGCT 3 cut(s) 464, 593, 1034
AluI AGCT 3 cut(s) 464, 593, 1034
Alw26I GTCTC 3 cut(s) 110, 837, 891
AlwI GGATC 1 cut(s) 419
AlwNI CAGNNNCTG 1 cut(s) 889
AoxI GGCC 2 cut(s) 704, 1101
ApeKI GCWGC 2 cut(s) 554, 593
ApoI RAATTY 2 cut(s) 27, 1049
AsiGI ACCGGT 1 cut(s) 646
Asp700I GAANNNNTTC 1 cut(s) 734
AspA2I CCTAGG 1 cut(s) 1087
AspS9I GGNCC 2 cut(s) 70, 705
AsuHPI GGTGA 1 cut(s) 1345
AvaII GGWCC 1 cut(s) 70
AvrII CCTAGG 1 cut(s) 1087
AxyI CCTNAGG 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 1103
BbsI GAAGAC 2 cut(s) 44, 1368
BbvI GCAGC 2 cut(s) 541, 580
BccI CCATC 1 cut(s) 554
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 2 cut(s) 783, 1413
BclI TGATCA 4 cut(s) 3, 157, 1006, 1234
BcoDI GTCTC 3 cut(s) 110, 837, 891
BcuI ACTAGT 1 cut(s) 1270
BfaI CTAG 2 cut(s) 1088, 1271
BfmI CTRYAG 1 cut(s) 1188
BfrI CTTAAG 1 cut(s) 296
BfuAI ACCTGC 2 cut(s) 519, 1195
BglII AGATCT 1 cut(s) 748
BisI GCNGC 2 cut(s) 555, 594
BlnI CCTAGG 1 cut(s) 1087
BlsI GCNGC 2 cut(s) 556, 595
BmcAI AGTACT 1 cut(s) 1082
Bme1390I CCNGG 2 cut(s) 783, 1413
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 705
BmiI GGNNCC 2 cut(s) 72, 707
BmrFI CCNGG 2 cut(s) 783, 1413
BmsI GCATC 2 cut(s) 79, 371
BpiI GAAGAC 2 cut(s) 44, 1368
BpmI CTGGAG 2 cut(s) 765, 1395
BpuEI CTTGAG 3 cut(s) 140, 489, 830
BsaJI CCNNGG 4 cut(s) 782, 1066, 1087, 1200
BsaWI WCCGGW 1 cut(s) 646
Bsc4I CCNNNNNNNGG 1 cut(s) 1418
Bse118I RCCGGY 1 cut(s) 646
Bse21I CCTNAGG 1 cut(s) 96
Bse3DI GCAATG 4 cut(s) 393, 450, 789, 1311
BseBI CCWGG 2 cut(s) 783, 1413
BseDI CCNNGG 4 cut(s) 782, 1066, 1087, 1200
BseGI GGATG 2 cut(s) 94, 988
BseLI CCNNNNNNNGG 1 cut(s) 1418
BseMI GCAATG 4 cut(s) 393, 450, 789, 1311
BseMII CTCAG 3 cut(s) 25, 87, 429
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 2 cut(s) 541, 580
BsgI GTGCAG 2 cut(s) 542, 547
BshFI GGCC 2 cut(s) 706, 1103
BshTI ACCGGT 1 cut(s) 646
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 2 cut(s) 56, 577
BslI CCNNNNNNNGG 1 cut(s) 1418
BsmAI GTCTC 3 cut(s) 110, 837, 891
BsmFI GGGAC 2 cut(s) 56, 577
BsmI GAATGC 3 cut(s) 734, 736, 1378
BsnI GGCC 2 cut(s) 706, 1103
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 1 cut(s) 1331
BspANI GGCC 2 cut(s) 706, 1103
BspCNI CTCAG 3 cut(s) 24, 88, 430
BspHI TCATGA 2 cut(s) 82, 160
BspLI GGNNCC 2 cut(s) 72, 707
BspMAI CTGCAG 1 cut(s) 1192
BspMI ACCTGC 2 cut(s) 519, 1195
BspPI GGATC 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 296
BsrDI GCAATG 4 cut(s) 393, 450, 789, 1311
BsrFI RCCGGY 1 cut(s) 646
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 4 cut(s) 782, 1066, 1087, 1200
BssMI GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
BssNAI GTATAC 1 cut(s) 766
BssT1I CCWWGG 3 cut(s) 1066, 1087, 1200
Bst1107I GTATAC 1 cut(s) 766
Bst2UI CCWGG 2 cut(s) 783, 1413
Bst4CI ACNGT 2 cut(s) 520, 885
BstAFI CTTAAG 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 1180, 1350
BstDEI CTNAG 6 cut(s) 11, 53, 96, 438, 1147, 1391
BstDSI CCRYGG 1 cut(s) 1200
BstF5I GGATG 2 cut(s) 94, 988
BstKTI GATC 7 cut(s) 6, 160, 427, 751, 1009, 1109, 1237
BstMAI GTCTC 3 cut(s) 110, 837, 891
BstMBI GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
BstMWI GCNNNNNNNGC 4 cut(s) 590, 650, 712, 1328
BstNI CCWGG 2 cut(s) 783, 1413
BstNSI RCATGY 4 cut(s) 152, 772, 1174, 1352
BstSCI CCNGG 2 cut(s) 781, 1411
BstSFI CTRYAG 1 cut(s) 1188
BstSLI GKGCMC 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 541, 580
BstV2I GAAGAC 2 cut(s) 44, 1368
BstX2I RGATCY 2 cut(s) 424, 748
BstYI RGATCY 2 cut(s) 424, 748
BstZ17I GTATAC 1 cut(s) 766
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 706, 1103
BtgI CCRYGG 1 cut(s) 1200
BtsCI GGATG 2 cut(s) 94, 988
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 5 cut(s) 213, 525, 530, 566, 1158
BveI ACCTGC 2 cut(s) 519, 1195
Cac8I GCNNGC 2 cut(s) 1180, 1350
CaiI CAGNNNCTG 1 cut(s) 889
CciI TCATGA 2 cut(s) 82, 160
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 2 cut(s) 70, 705
Csp6I GTAC 5 cut(s) 255, 494, 577, 674, 1081
CspAI ACCGGT 1 cut(s) 646
CviQI GTAC 5 cut(s) 255, 494, 577, 674, 1081
DdeI CTNAG 6 cut(s) 11, 53, 96, 438, 1147, 1391
DpnI GATC 7 cut(s) 5, 159, 426, 750, 1008, 1108, 1236
DpnII GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
EaeI YGGCCR 1 cut(s) 1101
Eco130I CCWWGG 3 cut(s) 1066, 1087, 1200
Eco32I GATATC 1 cut(s) 1262
Eco47I GGWCC 1 cut(s) 70
Eco57I CTGAAG 3 cut(s) 56, 731, 911
Eco81I CCTNAGG 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 781, 1411
EcoRV GATATC 1 cut(s) 1262
EcoT14I CCWWGG 3 cut(s) 1066, 1087, 1200
EcoT22I ATGCAT 3 cut(s) 364, 736, 1350
ErhI CCWWGG 3 cut(s) 1066, 1087, 1200
FaqI GGGAC 2 cut(s) 56, 577
FauNDI CATATG 5 cut(s) 358, 616, 1045, 1318, 1368
FbaI TGATCA 4 cut(s) 3, 157, 1006, 1234
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 2 cut(s) 555, 594
FokI GGATG 2 cut(s) 101, 995
Fsp4HI GCNGC 2 cut(s) 555, 594
FspBI CTAG 2 cut(s) 1088, 1271
GluI GCNGC 2 cut(s) 555, 594
GsuI CTGGAG 2 cut(s) 765, 1395
HaeIII GGCC 2 cut(s) 706, 1103
HapII CCGG 1 cut(s) 647
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 462, 1032
HinfI GANTC 3 cut(s) 122, 164, 353
HpaII CCGG 1 cut(s) 647
HphI GGTGA 1 cut(s) 1345
Hpy166II GTNNAC 3 cut(s) 336, 674, 766
Hpy188I TCNGA 6 cut(s) 127, 352, 753, 858, 881, 891
Hpy188III TCNNGA 8 cut(s) 83, 95, 161, 632, 937, 1010, 1232, 1307
Hpy8I GTNNAC 3 cut(s) 336, 674, 766
HpyAV CCTTC 6 cut(s) 442, 800, 867, 886, 942, 1109
HpyCH4III ACNGT 2 cut(s) 520, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 590, 650, 712, 1328
HpyF3I CTNAG 6 cut(s) 11, 53, 96, 438, 1147, 1391
Ksp22I TGATCA 4 cut(s) 3, 157, 1006, 1234
Kzo9I GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
LmnI GCTCC 4 cut(s) 97, 1204, 1357, 1414
Lsp1109I GCAGC 2 cut(s) 541, 580
LweI GCATC 2 cut(s) 79, 371
MaeI CTAG 2 cut(s) 1088, 1271
MaeIII GTNAC 2 cut(s) 914, 994
MalI GATC 7 cut(s) 5, 159, 426, 750, 1008, 1108, 1236
MboI GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
MboII GAAGA 6 cut(s) 49, 484, 739, 838, 1025, 1373
MfeI CAATTG 1 cut(s) 242
MflI RGATCY 2 cut(s) 424, 748
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 1103
MluCI AATT 6 cut(s) 27, 242, 325, 377, 1049, 1340
MluNI TGGCCA 1 cut(s) 1103
MlyI GAGTC 3 cut(s) 131, 173, 362
MmeI TCCRAC 1 cut(s) 1008
Mox20I TGGCCA 1 cut(s) 1103
Mph1103I ATGCAT 3 cut(s) 364, 736, 1350
MroXI GAANNNNTTC 1 cut(s) 734
MscI TGGCCA 1 cut(s) 1103
MseI TTAA 3 cut(s) 297, 303, 1076
MslI CAYNNNNRTG 3 cut(s) 87, 1097, 1371
Msp20I TGGCCA 1 cut(s) 1103
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 2 cut(s) 783, 1413
MunI CAATTG 1 cut(s) 242
Mva1269I GAATGC 3 cut(s) 734, 736, 1378
MvaI CCWGG 2 cut(s) 783, 1413
MwoI GCNNNNNNNGC 4 cut(s) 590, 650, 712, 1328
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 5 cut(s) 358, 616, 1045, 1318, 1368
NdeII GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
NlaIV GGNNCC 2 cut(s) 72, 707
NmuCI GTSAC 1 cut(s) 994
NsiI ATGCAT 3 cut(s) 364, 736, 1350
NspI RCATGY 4 cut(s) 152, 772, 1174, 1352
PaeI GCATGC 1 cut(s) 1352
PagI TCATGA 2 cut(s) 82, 160
PaqCI CACCTGC 1 cut(s) 519
PciI ACATGT 2 cut(s) 148, 768
PctI GAATGC 3 cut(s) 734, 736, 1378
PdmI GAANNNNTTC 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 646
PkrI GCNGC 2 cut(s) 556, 595
PleI GAGTC 3 cut(s) 130, 172, 361
PpsI GAGTC 3 cut(s) 130, 172, 361
PscI ACATGT 2 cut(s) 148, 768
PsiI TTATAA 1 cut(s) 137
Psp6I CCWGG 2 cut(s) 781, 1411
PspGI CCWGG 2 cut(s) 781, 1411
PspN4I GGNNCC 2 cut(s) 72, 707
PspPI GGNCC 2 cut(s) 70, 705
PstI CTGCAG 1 cut(s) 1192
PstNI CAGNNNCTG 1 cut(s) 889
PsuI RGATCY 2 cut(s) 424, 748
RsaI GTAC 5 cut(s) 256, 495, 578, 675, 1082
RsaNI GTAC 5 cut(s) 255, 494, 577, 674, 1081
RseI CAYNNNNRTG 3 cut(s) 87, 1097, 1371
SaqAI TTAA 3 cut(s) 297, 303, 1076
SatI GCNGC 2 cut(s) 555, 594
Sau3AI GATC 7 cut(s) 3, 157, 424, 748, 1006, 1106, 1234
Sau96I GGNCC 2 cut(s) 70, 705
ScaI AGTACT 1 cut(s) 1082
SchI GAGTC 3 cut(s) 131, 173, 362
ScrFI CCNGG 2 cut(s) 783, 1413
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 2 cut(s) 79, 371
SfcI CTRYAG 1 cut(s) 1188
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 3 cut(s) 87, 1097, 1371
SmlI CTYRAG 4 cut(s) 119, 296, 504, 845
SmoI CTYRAG 4 cut(s) 119, 296, 504, 845
SpeI ACTAGT 1 cut(s) 1270
SphI GCATGC 1 cut(s) 1352
Sse9I AATT 6 cut(s) 27, 242, 325, 377, 1049, 1340
SsiI CCGC 1 cut(s) 1331
SspMI CTAG 2 cut(s) 1088, 1271
StyD4I CCNGG 2 cut(s) 781, 1411
StyI CCWWGG 3 cut(s) 1066, 1087, 1200
TaaI ACNGT 2 cut(s) 520, 885
TaqII GACCGA 1 cut(s) 58
TasI AATT 6 cut(s) 27, 242, 325, 377, 1049, 1340
TatI WGTACW 2 cut(s) 673, 1080
Tru1I TTAA 3 cut(s) 297, 303, 1076
Tru9I TTAA 3 cut(s) 297, 303, 1076
TscAI CASTG 5 cut(s) 220, 525, 530, 573, 1165
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 2 cut(s) 554, 593
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 7 cut(s) 159, 461, 778, 1062, 1073, 1333, 1385
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 5 cut(s) 220, 525, 530, 573, 1165
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 2 cut(s) 27, 1049
XceI RCATGY 4 cut(s) 152, 772, 1174, 1352
XmaJI CCTAGG 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 734
XspI CTAG 2 cut(s) 1088, 1271
ZrmI AGTACT 1 cut(s) 1082
Zsp2I ATGCAT 3 cut(s) 364, 736, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.