RchiOBHm_Chr5g0079271

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000071
Physical Location & Seq
Forward (+)
84935869 .. 84939522
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35370

Sequence Viewer

Length: 3399 bp
ATGGTGGATTTGACTCTTACCGACGGTAATACTGGAGGACGTGGTGGTAGTCAAAGAGGCAAAGCGGCAGTCCAATTGGTGCCAGAACCTGACTTCTACGGCATTGCAGGGCAAGATCCCTCACAGGGTAATGTTTCCTTGACTAAGGGAACTCGAGGTAAAATTGGTGTTGCTTTACATGTTTCTGACTTTACTGGTAGTGATACATGGATAATTGATTCTGGTGCGTCTGACCATATGACCTATGATAAGTCCTTCTTTATGTCTATGTCATCCCCTTCTATATCCCATGTTTCTAATGCAAATGGTGCGTCTTTTCCAGTCTTAGGTATTGGGTCTGTTCAGGTTACACCATCCATTGTATTGCATGATGTGCTTTATGTACCTTCATTGTCTCATCATCTTTTGTCTGTATCCCAGTTGGGGTCACAAAATAAATGCTCTGTAACCTTTTATCCAATGTATGTTATTTTTCATAATCTGTGCACTAGGGTGATCATGGGCAAGGGAGACCTGAGGGGGAGACTGTTTCACTTGGATTGCATGTACGCAGAGCCAACACAAGCACCGGAACAGCCTTTAGCCCTGACATTGAATTCTGACAGATTAAGTGAGCTATGGTTATGGCATAGGCGTTTGGGTTATCCATCTTTTGGAGTTATGAAGAAGTCCATGCCTTCATTGTTTTTGGGAGTTAGTGAGTCTAGCCTGCATTGTGAAACTTGTGCTTTGGCTAAGAGTCATAGGTCTAGTTATCCTTCGAGTTTTCATTCTAGTACTATGCCTTTTGAGTTAATTCATTCAGATGTATGGGGTCCTTCTAAACATTCTACCCTTTCTGGAATGCGATATTTTGTGTTATTCATTGATGACTTTACTCGATTATCCTGGGTGGTTTTACTTAAGTCCAAAGACTCTGTTTTCTCTGCTTTTACAGCATTCCATAAGCTTGTTCGTACTCAATATGATGCTCATGTTAAGGTCTTTCGTTCCGATAATGGGGGTGAGTTTGTCAATCATTCTTTTCATGAATATTTCCAAGACCATGGCATCATACATCAAACTTCATGCTCACAGACACCTGAGCAAAATGGGGTGTCTGAACGGAAAAATCGTCATCTTCTGGACATGGCTCGGTCTCTTCTACTTAGTGCTAACATGCCTAAGTATCTTTGGGGAGAGGCCGTATTGTGTGCTTCTCATCTAATCAATCGTCTTCCGTCTGTCCCTCTTCAAGGTCGTGTCCCACTTGAGGTCCTGTCTAACTATGTTTCTATTCCATCTTCTAATACTCTTCCTGCTCGTGTCTTTGGTTGTATTGCTTATGTTCATCTATATAAGAATCAGAGGTCAAAGTTGGATGCTAGAGCCCTTAAGTGTGTGTTTGTGGAGTATAGTTCTCATCAGAAAGGTTACAAATGTTATCATCCTCAATCCCAGAAGTTTTATGTCACCATGGATGTTACGTTCAGTGAAGATGCATGTTATTTTTTACCTCCTGTGACTCCTTGTCAGGGGGAGAAGTCTTGTTATTATGAAGATTTGTTTAGTGGGCAAGATGGGAGACTATCATCCGAGTTCTCAAGGCTGGAGTGTTTTGGAACAGAACTTGCAAAAGAGGACAGCAGCCCACCGAATAGAGAAGAGAATTTGGGTGGAACGGAACTTGAAAAAGAGGACAGCAGCCCACCGAATGGAGAAGAGAATTTGGGTAGTCAGTTACTGACCAGTCTATCGGATCAGTCTGACCGGTTGGTTGAAGAGGAAGTTTCTGATTATGTTTCCGATGAAATTCTCGATGTCACCAATGCCGTTTTGAACAGTTCTTCTGTCTCTCCCTCTCCTTCAGTGGTCTCGCCTGCTCAATCATCACCCGAGGATGCAATGAGGGATGAGAAGTGGATGCAAGCAATGACCGTCGAAATGGATGCTCTTGAGAAGAATTGTACGTGGGAGCTAGTATCATTACCACCCGGGAAGAAGACAGTTGGTTGTCGGTGGGTGTATACTGTGAAACACAATTCAGATGGATCGGTGGACAAGTACAAGGCAAGACTAGTGGCTAAAGGCTATACTCAAAAGTATGGAGTGGATTATGATGAGACATTTGCACCAGTGGCCAAAATTAACACAATTCGGGTACTTCTTTCGTTAGCTGCTAATCTTGATTGGCCTTTACAACAGTTTGATGTTAAGAATGCATTCTTGCATGGTGATTTGCATGAAGAGGTTTATATGGATTTGCCTCCTGGATATGGTACTTCTACTGGAGAGCAGGTGGTGTGCAGATTGAAAAAATCTCTTTATGGTTTGAAGCAGTCTCCCAGAGCTTGGTTTGGCAGGTTCACCAAGTTCATGAAGAAAATTGGCTACCGGCAAAGCAATTCAGATCACACCTTGTTCCTTAAACATCGGTGTGGTAAAGTGACTGCCTTGATTATTTATGTTGATGACATGGTTGTTACAGGTGATGACTTTGAGGAGATTCAAAGGTTACAAGATCAGTTATCTTTAGAATTTGAGATGAAAGATTTGGGTAATTTGAAATATTTCTTGGGAATTGAAGTTGCTCGTGGGAAGGATTGTATTATGTTGAGTCAGAGAAAGTATGTTCTCGACTTGCTGGCAGAAACAGGTATGCTTGACAGTCAACCTGTTGATACTCCTATTGAGCAAAACCATAGGTTAGCAGAGTACTTGGATCAAGTACCTACGAATAAAGCAAGATACCAGAGGTTAGTTGGCCGGTTGATTTATTTATCCCACACTAGACCAGATTTAGCCTATGCTGTTAGTGTGGTGAGTCAGTTTATGCATAATCCCAGTAAGACTCATATGGATGCTGTATTTCGTATTTTGCGGTATTTAAAGTCTGCTCCAGGGAAGGGATTACTCTTTTCAAAACACAGTCACTTGGATGTTTCCGGGTACACAGACGCGGACTGGGCAGGTAATATTACAGATCGCAGGTCTACTTCGGGCTACTTCACATTTGTGGGTGGTAACTTGGTTACTTGGAAGAGCAAGAAACAAAAGGTGGTGGCTCGTTCTAGTGCAGAAGCAGAGTATAAAGGAATGGCCCGAGGACTTTGTGAGTTGTTGTGGTTGAGAAATTTGTTGAGGGATTTGGGGTTCAGGCAGGAAAAGGCTATGCAGCTTTATTGTGATAATAAGGCTACAATTGAAATTGCTCACAATCCTGTTCAGCATGATCGCACAAAGCATGTGGAGGTAGATCGACACTTCATTAAAGAGAAGCTGGATGGACAGATCATTATGTTTCCCTTCGTTCCTACTGAAGAACAGTTGGCAGATATTCTTACAAAGGCTCTCTCAAGTAAAGCCTTCTATGACTCACTTGACAAGTTGGGCATCTGTGATCTGTATGCACCAACTTGA

Protein Analysis

1132

Amino Acids

127.14

Weight (kDa)

6.54

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 70 - 143 2.3e-19 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 197 - 247 2.1e-12 GAG-pre-integrase domain
rve PF00665 260 - 361 1e-10 Integrase core domain
SH3_retrovirus PF25597 439 - 496 3.9e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 649 - 891 8.1e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2266
Acc36I ACCTGC 4 cut(s) 2266, 2331, 2939, 2958
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 3 cut(s) 653, 1694, 2331
AccI GTMKAC 2 cut(s) 2006, 2972
AccII CGCG 1 cut(s) 2939
AciI CCGC 3 cut(s) 65, 2860, 2939
AclWI GGATC 4 cut(s) 110, 1746, 2038, 2709
AcoI YGGCCR 2 cut(s) 2118, 2743
AcsI RAATTY 6 cut(s) 595, 1648, 1705, 1791, 2516, 3112
AcuI CTGAAG 2 cut(s) 1830, 3318
AflII CTTAAG 2 cut(s) 902, 1373
AflIII ACRYGT 1 cut(s) 178
AgeI ACCGGT 1 cut(s) 1749
AhdI GACNNNNNGTC 1 cut(s) 1509
AhlI ACTAGT 1 cut(s) 2056
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 3 cut(s) 887, 2248, 2878
AjuI GAANNNNNNNTTGG 2 cut(s) 2537, 2569
AleI CACNNNNGTG 2 cut(s) 491, 2993
AloI GAACNNNNNNTCC 4 cut(s) 1451, 1483, 3116, 3148
AluBI AGCT 7 cut(s) 616, 949, 1957, 2156, 2330, 3157, 3259
AluI AGCT 7 cut(s) 616, 949, 1957, 2156, 2330, 3157, 3259
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 9 cut(s) 399, 504, 517, 1143, 1558, 1837, 1858, 2096, 2325
Alw44I GTGCAC 1 cut(s) 484
AlwI GGATC 4 cut(s) 110, 1746, 2038, 2709
AlwNI CAGNNNCTG 2 cut(s) 89, 1723
Ama87I CYCGRG 4 cut(s) 153, 1874, 1973, 3081
AoxI GGCC 5 cut(s) 1182, 2118, 2171, 2743, 3078
ApaLI GTGCAC 1 cut(s) 484
ApeKI GCWGC 4 cut(s) 1626, 1683, 2156, 3154
ApoI RAATTY 6 cut(s) 595, 1648, 1705, 1791, 2516, 3112
ArsI GACNNNNNNTTYG 4 cut(s) 54, 86, 296, 328
AsiGI ACCGGT 1 cut(s) 1749
Asp700I GAANNNNTTC 2 cut(s) 2201, 2549
AspS9I GGNCC 3 cut(s) 815, 1255, 3079
AsuC2I CCSGG 3 cut(s) 1974, 1975, 2926
AsuHPI GGTGA 9 cut(s) 505, 1016, 1444, 1795, 1863, 2225, 2338, 2480, 2812
AvaI CYCGRG 4 cut(s) 153, 1874, 1973, 3081
AvaII GGWCC 2 cut(s) 815, 1255
AxyI CCTNAGG 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 488
BalI TGGCCA 1 cut(s) 2120
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 1372
BauI CACGAG 2 cut(s) 1302, 2571
BbsI GAAGAC 2 cut(s) 1208, 1988
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 4 cut(s) 1638, 1695, 2143, 3166
BccI CCATC 6 cut(s) 361, 655, 1288, 1553, 2021, 3257
BceAI ACGGC 3 cut(s) 115, 1169, 1796
BcgI CGANNNNNNTGC 2 cut(s) 1910, 1944
BciT130I CCWGG 3 cut(s) 889, 2250, 2880
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 1 cut(s) 495
BcnI CCSGG 3 cut(s) 1974, 1975, 2926
BcoDI GTCTC 9 cut(s) 399, 504, 517, 1143, 1558, 1837, 1858, 2096, 2325
BcuI ACTAGT 1 cut(s) 2056
BfaI CTAG 9 cut(s) 489, 705, 750, 774, 1365, 1958, 2057, 2769, 3051
BfrI CTTAAG 2 cut(s) 902, 1373
BfuAI ACCTGC 4 cut(s) 2266, 2331, 2939, 2958
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 5 cut(s) 66, 1627, 1684, 2157, 3155
BlsI GCNGC 5 cut(s) 67, 1628, 1685, 2158, 3156
BmcAI AGTACT 2 cut(s) 778, 2696
Bme1390I CCNGG 6 cut(s) 889, 1974, 1975, 2250, 2880, 2926
Bme18I GGWCC 2 cut(s) 815, 1255
BmeRI GACNNNNNGTC 1 cut(s) 1509
BmeT110I CYCGRG 4 cut(s) 153, 1874, 1973, 3081
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 3 cut(s) 815, 1255, 3079
BmiI GGNNCC 2 cut(s) 81, 816
BmrFI CCNGG 6 cut(s) 889, 1974, 1975, 2250, 2880, 2926
BmrI ACTGGG 3 cut(s) 412, 2817, 2953
BmsI GCATC 9 cut(s) 958, 1059, 1351, 1468, 1870, 1893, 1918, 2830, 3381
BmuI ACTGGG 3 cut(s) 412, 2817, 2953
BpiI GAAGAC 2 cut(s) 1208, 1988
BpmI CTGGAG 4 cut(s) 54, 1610, 2289, 2862
Bpu10I CCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 4 cut(s) 1271, 1567, 1955, 3319
BpuMI CCSGG 3 cut(s) 1974, 1975, 2926
BsaAI YACGTR 1 cut(s) 1950
BsaI GGTCTC 3 cut(s) 504, 1143, 1858
BsaJI CCNNGG 7 cut(s) 888, 1045, 1455, 1875, 1973, 2879, 3082
BsaWI WCCGGW 2 cut(s) 568, 1749
BsaXI ACNNNNNCTCC 4 cut(s) 1170, 1200, 2262, 2292
Bse118I RCCGGY 3 cut(s) 1749, 2373, 2745
Bse1I ACTGG 9 cut(s) 37, 199, 320, 418, 1728, 2114, 2272, 2823, 2948
Bse21I CCTNAGG 1 cut(s) 515
Bse3DI GCAATG 3 cut(s) 102, 1890, 1917
BseBI CCWGG 3 cut(s) 889, 2250, 2880
BseDI CCNNGG 7 cut(s) 888, 1045, 1455, 1875, 1973, 2879, 3082
BseMI GCAATG 3 cut(s) 102, 1890, 1917
BseMII CTCAG 2 cut(s) 506, 1074
BseNI ACTGG 9 cut(s) 37, 199, 320, 418, 1728, 2114, 2272, 2823, 2948
BseRI GAGGAG 1 cut(s) 2495
BseSI GKGCMC 1 cut(s) 488
BseXI GCAGC 4 cut(s) 1638, 1695, 2143, 3166
BsgI GTGCAG 2 cut(s) 2305, 3075
Bsh1236I CGCG 1 cut(s) 2939
BshFI GGCC 5 cut(s) 1184, 2120, 2173, 2745, 3080
BshNI GGYRCC 1 cut(s) 79
BshTI ACCGGT 1 cut(s) 1749
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 4 cut(s) 153, 1874, 1973, 3081
BsiSI CCGG 6 cut(s) 569, 1750, 1974, 2374, 2746, 2925
BslFI GGGAC 2 cut(s) 1211, 1229
BsmAI GTCTC 9 cut(s) 399, 504, 517, 1143, 1558, 1837, 1858, 2096, 2325
BsmFI GGGAC 2 cut(s) 1211, 1229
BsmI GAATGC 4 cut(s) 849, 938, 2201, 2203
BsnI GGCC 5 cut(s) 1184, 2120, 2173, 2745, 3080
Bso31I GGTCTC 3 cut(s) 504, 1143, 1858
BsoBI CYCGRG 4 cut(s) 153, 1874, 1973, 3081
Bsp1286I GDGCHC 2 cut(s) 488, 1372
Bsp19I CCATGG 2 cut(s) 1045, 1455
BspACI CCGC 3 cut(s) 65, 2860, 2939
BspANI GGCC 5 cut(s) 1184, 2120, 2173, 2745, 3080
BspCNI CTCAG 2 cut(s) 507, 1075
BspFNI CGCG 1 cut(s) 2939
BspHI TCATGA 2 cut(s) 1027, 2355
BspLI GGNNCC 2 cut(s) 81, 816
BspMI ACCTGC 4 cut(s) 2266, 2331, 2939, 2958
BspPI GGATC 4 cut(s) 110, 1746, 2038, 2709
BspQI GCTCTTC 1 cut(s) 3014
BspT107I GGYRCC 1 cut(s) 79
BspTI CTTAAG 2 cut(s) 902, 1373
BspTNI GGTCTC 3 cut(s) 504, 1143, 1858
BsrDI GCAATG 3 cut(s) 102, 1890, 1917
BsrFI RCCGGY 3 cut(s) 1749, 2373, 2745
BsrI ACTGG 9 cut(s) 37, 199, 320, 418, 1728, 2114, 2272, 2823, 2948
BssAI RCCGGY 3 cut(s) 1749, 2373, 2745
BssECI CCNNGG 7 cut(s) 888, 1045, 1455, 1875, 1973, 2879, 3082
BssNAI GTATAC 1 cut(s) 2007
BssSI CACGAG 2 cut(s) 1302, 2571
BssT1I CCWWGG 2 cut(s) 1045, 1455
Bst1107I GTATAC 1 cut(s) 2007
Bst2BI CACGAG 2 cut(s) 1302, 2571
Bst2UI CCWGG 3 cut(s) 889, 2250, 2880
Bst6I CTCTTC 8 cut(s) 1146, 1236, 1299, 1638, 1695, 1755, 2220, 3014
BstAFI CTTAAG 2 cut(s) 902, 1373
BstAPI GCANNNNNTGC 2 cut(s) 308, 373
BstBAI YACGTR 1 cut(s) 1950
BstC8I GCNNGC 4 cut(s) 710, 1860, 1908, 2625
BstDEI CTNAG 7 cut(s) 144, 325, 515, 735, 1083, 1148, 1164
BstDSI CCRYGG 2 cut(s) 1045, 1455
BstENI CCTNNNNNAGG 1 cut(s) 1233
BstFNI CGCG 1 cut(s) 2939
BstMAI GTCTC 9 cut(s) 399, 504, 517, 1143, 1558, 1837, 1858, 2096, 2325
BstMWI GCNNNNNNNGC 5 cut(s) 308, 373, 935, 2117, 2945
BstNI CCWGG 3 cut(s) 889, 2250, 2880
BstNSI RCATGY 5 cut(s) 182, 547, 1162, 1485, 3227
BstSCI CCNGG 6 cut(s) 887, 1972, 1973, 2248, 2878, 2924
BstSLI GKGCMC 1 cut(s) 488
BstUI CGCG 1 cut(s) 2939
BstV1I GCAGC 4 cut(s) 1638, 1695, 2143, 3166
BstV2I GAAGAC 2 cut(s) 1208, 1988
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 1046
BstYI RGATCY 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 2007
Bsu36I CCTNAGG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 5 cut(s) 1184, 2120, 2173, 2745, 3080
BtgI CCRYGG 2 cut(s) 1045, 1455
BtrI CACGTC 1 cut(s) 41
BtsIMutI CAGTG 3 cut(s) 1477, 1854, 2121
BveI ACCTGC 4 cut(s) 2266, 2331, 2939, 2958
Cac8I GCNNGC 4 cut(s) 710, 1860, 1908, 2625
CaiI CAGNNNCTG 2 cut(s) 89, 1723
CciI TCATGA 2 cut(s) 1027, 2355
Cfr10I RCCGGY 3 cut(s) 1749, 2373, 2745
Cfr13I GGNCC 3 cut(s) 815, 1255, 3079
Cfr9I CCCGGG 1 cut(s) 1973
CseI GACGC 3 cut(s) 216, 300, 2945
CspAI ACCGGT 1 cut(s) 1749
CspCI CAANNNNNGTGG 4 cut(s) 2040, 2075, 3207, 3242
DdeI CTNAG 7 cut(s) 144, 325, 515, 735, 1083, 1148, 1164
DraI TTTAAA 1 cut(s) 2868
DriI GACNNNNNGTC 1 cut(s) 1509
EaeI YGGCCR 2 cut(s) 2118, 2743
Eam1104I CTCTTC 8 cut(s) 1146, 1236, 1299, 1638, 1695, 1755, 2220, 3014
Eam1105I GACNNNNNGTC 1 cut(s) 1509
EarI CTCTTC 8 cut(s) 1146, 1236, 1299, 1638, 1695, 1755, 2220, 3014
Eco130I CCWWGG 2 cut(s) 1045, 1455
Eco24I GRGCYC 1 cut(s) 1372
Eco31I GGTCTC 3 cut(s) 504, 1143, 1858
Eco47I GGWCC 2 cut(s) 815, 1255
Eco57I CTGAAG 2 cut(s) 1830, 3318
Eco81I CCTNAGG 1 cut(s) 515
Eco88I CYCGRG 4 cut(s) 153, 1874, 1973, 3081
EcoNI CCTNNNNNAGG 1 cut(s) 1233
EcoO109I RGGNCCY 2 cut(s) 815, 1255
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 3 cut(s) 887, 2248, 2878
EcoT14I CCWWGG 2 cut(s) 1045, 1455
EcoT22I ATGCAT 3 cut(s) 1483, 2203, 2817
EcoT38I GRGCYC 1 cut(s) 1372
ErhI CCWWGG 2 cut(s) 1045, 1455
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 2 cut(s) 1211, 1229
FauNDI CATATG 2 cut(s) 237, 2835
FbaI TGATCA 1 cut(s) 495
FblI GTMKAC 2 cut(s) 2006, 2972
Fnu4HI GCNGC 5 cut(s) 66, 1627, 1684, 2157, 3155
FriOI GRGCYC 1 cut(s) 1372
Fsp4HI GCNGC 5 cut(s) 66, 1627, 1684, 2157, 3155
FspBI CTAG 9 cut(s) 489, 705, 750, 774, 1365, 1958, 2057, 2769, 3051
GluI GCNGC 5 cut(s) 66, 1627, 1684, 2157, 3155
GsuI CTGGAG 4 cut(s) 54, 1610, 2289, 2862
HaeIII GGCC 5 cut(s) 1184, 2120, 2173, 2745, 3080
HapII CCGG 6 cut(s) 569, 1750, 1974, 2374, 2746, 2925
HgaI GACGC 3 cut(s) 216, 300, 2945
HincII GTYRAC 1 cut(s) 2651
HindII GTYRAC 1 cut(s) 2651
HindIII AAGCTT 1 cut(s) 947
HpaII CCGG 6 cut(s) 569, 1750, 1974, 2374, 2746, 2925
HphI GGTGA 9 cut(s) 505, 1016, 1444, 1795, 1863, 2225, 2338, 2480, 2812
Hpy166II GTNNAC 7 cut(s) 486, 2007, 2038, 2346, 2651, 2931, 2973
Hpy188III TCNNGA 8 cut(s) 840, 1028, 1124, 1796, 1934, 2165, 2356, 2615
Hpy8I GTNNAC 7 cut(s) 486, 2007, 2038, 2346, 2651, 2931, 2973
Hpy99I CGWCG 2 cut(s) 26, 1922
HpyCH4IV ACGT 3 cut(s) 40, 1466, 1949
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 373, 935, 2117, 2945
HpyF3I CTNAG 7 cut(s) 144, 325, 515, 735, 1083, 1148, 1164
HpySE526I ACGT 3 cut(s) 40, 1466, 1949
Ksp22I TGATCA 1 cut(s) 495
LguI GCTCTTC 1 cut(s) 3014
LmnI GCTCC 2 cut(s) 1954, 2881
Lsp1109I GCAGC 4 cut(s) 1638, 1695, 2143, 3166
LweI GCATC 9 cut(s) 958, 1059, 1351, 1468, 1870, 1893, 1918, 2830, 3381
MaeI CTAG 9 cut(s) 489, 705, 750, 774, 1365, 1958, 2057, 2769, 3051
MaeII ACGT 3 cut(s) 40, 1466, 1949
MfeI CAATTG 2 cut(s) 74, 3180
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 488, 1372
MlsI TGGCCA 1 cut(s) 2120
MluNI TGGCCA 1 cut(s) 2120
MlyI GAGTC 9 cut(s) 7, 710, 748, 908, 1498, 2605, 2812, 2824, 3347
MmeI TCCRAC 1 cut(s) 1338
Mox20I TGGCCA 1 cut(s) 2120
Mph1103I ATGCAT 3 cut(s) 1483, 2203, 2817
MroXI GAANNNNTTC 2 cut(s) 2201, 2549
MscI TGGCCA 1 cut(s) 2120
MslI CAYNNNNRTG 6 cut(s) 491, 804, 2415, 2838, 2916, 2993
Msp20I TGGCCA 1 cut(s) 2120
MspCI CTTAAG 2 cut(s) 902, 1373
MspI CCGG 6 cut(s) 569, 1750, 1974, 2374, 2746, 2925
MspR9I CCNGG 6 cut(s) 889, 1974, 1975, 2250, 2880, 2926
MunI CAATTG 2 cut(s) 74, 3180
Mva1269I GAATGC 4 cut(s) 849, 938, 2201, 2203
MvaI CCWGG 3 cut(s) 889, 2250, 2880
MvnI CGCG 1 cut(s) 2939
MwoI GCNNNNNNNGC 5 cut(s) 308, 373, 935, 2117, 2945
NciI CCSGG 3 cut(s) 1974, 1975, 2926
NcoI CCATGG 2 cut(s) 1045, 1455
NdeI CATATG 2 cut(s) 237, 2835
NlaIV GGNNCC 2 cut(s) 81, 816
NmuCI GTSAC 6 cut(s) 426, 1450, 1501, 1801, 2425, 2909
NsiI ATGCAT 3 cut(s) 1483, 2203, 2817
NspI RCATGY 5 cut(s) 182, 547, 1162, 1485, 3227
OliI CACNNNNGTG 2 cut(s) 491, 2993
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 2 cut(s) 1027, 2355
PaqCI CACCTGC 1 cut(s) 2266
PciI ACATGT 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 3014
PcsI WCGNNNNNNNCGW 1 cut(s) 1111
PctI GAATGC 4 cut(s) 849, 938, 2201, 2203
PdmI GAANNNNTTC 2 cut(s) 2201, 2549
PfeI GAWTC 3 cut(s) 218, 1342, 2485
PflMI CCANNNNNTGG 3 cut(s) 653, 1694, 2331
PfoI TCCNGGA 1 cut(s) 2248
PinAI ACCGGT 1 cut(s) 1749
PkrI GCNGC 5 cut(s) 67, 1628, 1685, 2158, 3156
PleI GAGTC 9 cut(s) 7, 709, 747, 908, 1498, 2604, 2811, 2824, 3347
PpsI GAGTC 9 cut(s) 7, 709, 747, 908, 1498, 2604, 2811, 2824, 3347
Ppu21I YACGTR 1 cut(s) 1950
PpuMI RGGWCCY 2 cut(s) 815, 1255
PscI ACATGT 1 cut(s) 178
Psp5II RGGWCCY 2 cut(s) 815, 1255
Psp6I CCWGG 3 cut(s) 887, 2248, 2878
PspGI CCWGG 3 cut(s) 887, 2248, 2878
PspN4I GGNNCC 2 cut(s) 81, 816
PspPI GGNCC 3 cut(s) 815, 1255, 3079
PspPPI RGGWCCY 2 cut(s) 815, 1255
PspXI VCTCGAGB 1 cut(s) 153
PstNI CAGNNNCTG 2 cut(s) 89, 1723
PsuI RGATCY 1 cut(s) 115
RseI CAYNNNNRTG 6 cut(s) 491, 804, 2415, 2838, 2916, 2993
SapI GCTCTTC 1 cut(s) 3014
SatI GCNGC 5 cut(s) 66, 1627, 1684, 2157, 3155
Sau96I GGNCC 3 cut(s) 815, 1255, 3079
ScaI AGTACT 2 cut(s) 778, 2696
SchI GAGTC 9 cut(s) 7, 710, 748, 908, 1498, 2605, 2812, 2824, 3347
ScrFI CCNGG 6 cut(s) 889, 1974, 1975, 2250, 2880, 2926
SduI GDGCHC 2 cut(s) 488, 1372
SfaNI GCATC 9 cut(s) 958, 1059, 1351, 1468, 1870, 1893, 1918, 2830, 3381
Sfr274I CTCGAG 1 cut(s) 153
SinI GGWCC 2 cut(s) 815, 1255
SlaI CTCGAG 1 cut(s) 153
SmaI CCCGGG 1 cut(s) 1975
SmiMI CAYNNNNRTG 6 cut(s) 491, 804, 2415, 2838, 2916, 2993
SmlI CTYRAG 7 cut(s) 153, 902, 1250, 1373, 1582, 1934, 3334
SmoI CTYRAG 7 cut(s) 153, 902, 1250, 1373, 1582, 1934, 3334
SpeI ACTAGT 1 cut(s) 2056
SsiI CCGC 3 cut(s) 65, 2860, 2939
SspI AATATT 3 cut(s) 1034, 2549, 2956
SspMI CTAG 9 cut(s) 489, 705, 750, 774, 1365, 1958, 2057, 2769, 3051
StyD4I CCNGG 6 cut(s) 887, 1972, 1973, 2248, 2878, 2924
StyI CCWWGG 2 cut(s) 1045, 1455
TaiI ACGT 3 cut(s) 43, 1469, 1952
TaqI TCGA 7 cut(s) 154, 761, 880, 1797, 1920, 2616, 3238
TaqII GACCGA 1 cut(s) 1125
TatI WGTACW 3 cut(s) 776, 2043, 2694
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 218, 1342, 2485
TscAI CASTG 3 cut(s) 1477, 1854, 2121
TseFI GTSAC 6 cut(s) 426, 1450, 1501, 1801, 2425, 2909
TseI GCWGC 4 cut(s) 1626, 1683, 2156, 3154
Tsp45I GTSAC 6 cut(s) 426, 1450, 1501, 1801, 2425, 2909
TspGWI ACGGA 3 cut(s) 1120, 1209, 1676
TspMI CCCGGG 1 cut(s) 1973
TspRI CASTG 3 cut(s) 1477, 1854, 2121
Van91I CCANNNNNTGG 3 cut(s) 653, 1694, 2331
Vha464I CTTAAG 2 cut(s) 902, 1373
VneI GTGCAC 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 815, 1255
XagI CCTNNNNNAGG 1 cut(s) 1233
XapI RAATTY 6 cut(s) 595, 1648, 1705, 1791, 2516, 3112
XceI RCATGY 5 cut(s) 182, 547, 1162, 1485, 3227
XcmI CCANNNNNNNNNTGG 1 cut(s) 2738
XhoI CTCGAG 1 cut(s) 153
XmaI CCCGGG 1 cut(s) 1973
XmiI GTMKAC 2 cut(s) 2006, 2972
XmnI GAANNNNTTC 2 cut(s) 2201, 2549
XspI CTAG 9 cut(s) 489, 705, 750, 774, 1365, 1958, 2057, 2769, 3051
ZrmI AGTACT 2 cut(s) 778, 2696
Zsp2I ATGCAT 3 cut(s) 1483, 2203, 2817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.