MD06G1120600.v1.1

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000071
Physical Location & Seq
Forward (+)
26107334 .. 26113910
6577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1120600.v1.1.491

Sequence Viewer

Length: 4371 bp
ATGGTGGTTCTAAGCCTTCAACCTGTCCAATTTACAACGCCTAAACACTTATCATCATGTAAACCGAGTGTTTGTGGTTGTTCCGTCCGATATCGGATTTCAGAGCCATTAGGAGAGCCAGCTCCAATGGGGAAAAAAACAATGTACAATGATGGGTTTTTCGAGAGGGGATTCATGACTCTGTTTGCTCGAAAAATGATGAAGTCTGCAGCTCCGGTGAATGCGAAAACTGAGACAAAGGAGAAGCCGTGGTCTCGGAGTCAGCAACAGAAAGTGGTTCGAGAAGTGCTCTTGTCTATGCTGCCTCCAGGTGCGCCAGAGCAGAAACTATTTCAACCAACAAAGTGGGCAGCTGAGTTCAATGCTGCATTGACAGTTCCTTTCTTCCACTGGTTGGTTGGGCCTTCTGAGGTTGAAGAAGTTGAGATAAATGGGGTGAAGCAAAGCAGTGGAGTTCATATAAAGAAATGCAGACCTCATACCACGGATACAACCATTCTTTTAAAGCGTGCTGAAGAAGACAAGGTATTCCAGTTGCTTGCCAGCATTGGACCAGAGTATGAAGACCTTAAAAGCCATCTCTTGATGACACCTGAACTTCCCACCTTCCAGATGGTCTGCAATGTTATTCAACGCGAAGAAATAAGAAAGAATGTCATGAATGCAGACGTGGTCATTGGAGAATCAGATCTTAGGCCATCAGAAGCGAGAGCCTTCACCTTTTCCAGACCATACAAGGGAAAACGACCAGACTTAAAGTGCTCTCACTGTGTGAAGATAGGTCGTCCAGGCGTTGGACACATAAAAGAAAAATGCTGGATTCTTCATCCAGAACTTAAACCCAAGTTCAACGATGATGGAAGAGCACCAAGAAACCAAATGAAGCCTGCCTACACTCCTAGAGGAAACCTTGTTTGCGACAATGCCTCCAATAATGTGATGAACTCTTCTTCTAGTCCCATTACTCTCATAAATGAGTTTCCTAACTTCTTGCAACAGAAACAAGGAACAACAAATCATGAAAGTCCCTCAGCCATGCTCGGCAAGTTTGCTGGCTTCCTCGAACAATCAAGCTCAGTGTCACAAAAAGACGTGCCAGGTATTGTTGCAACTATTTCCACTGCATTGGATCTTAGTAGTAGTCGTGATTTTTGGATAGTCGACTCAGGAGCTTCTGATCACATGACTAACGATGCATCTTTACTAAATGATTTTCAAAACTTTTCCACTCCTTCTTTTGTCTCCGTAGCTAATGGCACCAAAGTTCGTATAGTCGGCATAGGGAAACTCAAAATCTTCTCAAGTCAAAAAGAATCCATTGTCATGCATGTCCCTTCTTTTCCTTTCAAACTGCTATCAGTAGGAAGAATAACACAATTGCTCAATTGTCTTGCAATTTTTTCCACTCACTGTGTGATTTTCCAGGATCGAGTTTCGAAGGAAACGATTGGTAAAGGTTTCTTCCTCAATGGCCTGTACTATGTCAACTTCTCTTCAAGTTTTCCAAAGAGTTTCTACGTAAACTCAAACACAGTAAACAAGGAGCAGCTGTGGCACATGAGATTAGCTCACCCTTCTTCACTTGTTATGTCTTTTATTTTTCCTAGTTTCTGTAAAGAGATCCCAGATTGTGAAGTTTGTCACAAATCTAAGTTTACCAGGTTACCCTTTCCCCACTCTCTCTTTAGAGCTACCAAAGCTTTTGAAATTGTCCATTCCGACATTTGGGGACCTGCAACCATAGAGTCTTTTGATGGTTACAGATTCTATATATCTTTCATTGATGATTTCTCTAGAACTACCTTTGTGTATTTACTTAAATTCAAGCACGAAGCTTTCCAGTGTTTCAAAAATTTTCATAACTTAGTTCAAAATCATTTTAACTCTAATATTGGCATCTTAAGGTCTGATAATGGCTCTGAGTACACTTCCAAAATCATGACAACTTACTTAAGTGAGCATGGAATTTTGCAACAAACAAGTTGTGTAGGAACGCCACAACAAAATGGCATTTCAGAAAGGAAGAACCGAGATTTGCTTGAGAAAACACAATCACTAATGTTACAAGCAAATCTTCCAAAGAAGTTCTGGTCTCAAGCTATACAAACCGCTGCATACATCATCAATCGCCTTCCTAGCAGAATCTTGAAGTTTAAATCTCCTTACCAAATTCTCAAGGGGAAAGATATTGACATAAGTCATCTCCGTGTGTTTGGATGTGTATGCTATGTGCACATTCAAACTATTCACAGAGATAAACTTGATCCAAGGGCTATCAAGTGTGCATTCGTAGGCTACTCAACTTCCCAAAAGGGATACAAATGCTATGATTCTAAGACCAGAAAGGTGTTTGTATCCAGGGATGTACGGTTTGATGAGACACACTCCTTCTTTCAGAAGCATGATGATGAACCTCAGGGGGAGCTCTCATATGAAGTGTTTCCCACTCCTCTCTTCCTTGATGATCCAAGGCTGTCAACACCGCACAATGAAGCTGTTGAGATAGAGCCAGCTCCTGAAAATATGGAAGCAAATGATGAAGAAGTCCTCCACGAAAGGAGAGAGCCCCAACTTGAACAACCTCCTCCTCCACGAAGGAATCCTTCTCGAAACCGACAACCTCCAGCCAGGCTTGATGATTTTGAAATCTACACGCCTAGATATCCTATTGGACAAATGGTTTACCACAGCAAGATCTCCCCATCTCATGCAGCCTTTGTTACTAAGTTATCTCAAAACTCTGAGCCTCGATCCTTCGAAAAGGCAAACCAATCTCCTGTATGGAGGCGTGCCATGCTTGAAGAACTGAAGGCACTCGATGAAAATAAAACTTGGAGCATTGTTTCTCTGCCAAAAGATCAACGAGTGGTTGGGGCAAGATGGATTTACAAGACAAAATTTCACTCAGATGGAACCATACAGCGGCACAAGGCAAGGTTAGTTGCAAGGGGCTTCACGCAAACCTTCGGGGTAGATTACAAAGAAACCTTTGCCCCGGTGGCAAAGATGAATACAGTTAGAGTGCTTCTTTCGGTGGCTATCAATTCACACTGGTCTCTACATCAAATGGATGTAAAAAACGCTTTCCTACATGGGGAGTTAGAAGAAGAGGTGTACATGCACCTACCACCTAGTCATGAACGAGAGAAGGAACAAGGAATAGTCTGCAAGCTTCACAAGGCCATTTACGGTCTCAAGCAATCTCCAAGGGCTTGGTACTCAAAACTGAACTCAGTCCTTATGTCATCCGGTTTTAAACGAAGCAATGCAGATTCCTCAATGTTCACTAGGATTGGTACGCATGGAAGATTGGTGGTACTTGTTTATGTTGATGATCTTATTGTCACAGGAGATAATCCAGAAGAAATAACTGCTCTCAAAGCTACCTTACATTCAACCTTCTCAATCAAAGATCTTGGCAGACTCCGATACTTTCTTGGAATCGAAATGGATCAATCTCCAACTGGTCTGTTTCTAAATCAACAAAAGTACGTTCTCGATCTTTTAGAAGAAGCCAATATGATGCATTGCAAGCCTGCCAAAACACCACTACCAACAAATGTCAAGGATGAAACCTCAGGCATCCAGCTTGAAAATGCATCGGAGTATCAAAGATTGGTAGGAAAACTTATCTATTTGACTATAACCCGACCTAACATTGCTTATGCTGTTAGCTTTGTCAGCCAGTTCATGCATTCTCCCTCATCTCATCATCTGCAACTAGTTAAAAGGATCCTTCGGTACTTGAAAAGCTCAATGTCTACCGGGATTTTCATGCAAAACAACGGACACTTCACCATTGAAGGATACACAGACTCTGATTGGGCAGGCAACGTACTTGATCGCAAGTCCACAACTGGTTACTGCACTCTTGTTGGTGGTAACCTCGTCACGTGGAAGAGCAAAAAGCAATCCGTTGTAGCTCGGTCAAGCGCAGAAGCTGAGTACCGTGCAATGGCATCTACTGCATGCGAACTAATTTGGCTAAAGAGTTTGCTACGTGATCTTGAAATCATCTCTTCCAAGCCAATTCTCCTCCACTGTGACAATCAAGCAGCGATGCATATAGCTGCCAACCCCGTCTTCCATGAACACACAAAGCATATCGAGGTTGACTGTCATTTCGTCTACAACCAAGTTCAGTCCAAACTGTTACAAACTGTATTTGTTCGCTCCAGTGATCAACTTGCCGATGTAATGTGTGTGAATATGTGCAAGTTTCCTACACAAGATTTCTTCACCAATGAATTTGGACTTTCCTTAACCATGATTCCAAGTATGTCTGATTATTTTGGTTATATTGTGCCCAAAAACTGGGATTTAAGGCTGAAATTCTTGTATAATTTGATGCAACACATTCCAAGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 rve

Protein Analysis

1457

Amino Acids

165.67

Weight (kDa)

9.02

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 385 - 459 3.3e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 491 - 551 7.9e-08 GAG-pre-integrase domain
rve PF00665 565 - 666 5.2e-11 Integrase core domain
SH3_retrovirus PF25597 740 - 803 5.3e-27 Retroviral polymerase SH3-like domain
RVT_2 PF07727 942 - 1184 1.6e-78 Reverse transcriptase (RNA-dependent DNA polymerase)
D27-like_C PF13225 1399 - 1430 3.3e-09 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1741
AccB1I GGYRCC 1 cut(s) 1256
AccB7I CCANNNNNTGG 3 cut(s) 394, 794, 4314
AccI GTMKAC 3 cut(s) 1161, 3761, 4128
AccII CGCG 1 cut(s) 636
AciI CCGC 3 cut(s) 2109, 2483, 2923
AclWI GGATC 9 cut(s) 1137, 1434, 1615, 2258, 2459, 2745, 3459, 3727, 3740
AcsI RAATTY 7 cut(s) 1820, 1852, 1965, 2169, 2897, 4247, 4331
AcuI CTGAAG 2 cut(s) 534, 2828
AcvI CACGTG 1 cut(s) 3894
AdeI CACNNNGTG 2 cut(s) 772, 1414
AfiI CCNNNNNNNGG 8 cut(s) 394, 737, 794, 1725, 2922, 3094, 3955, 4314
AflII CTTAAG 2 cut(s) 1900, 1951
AhlI ACTAGT 1 cut(s) 3721
AjiI CACGTC 2 cut(s) 670, 1093
AjnI CCWGG 7 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627
AjuI GAANNNNNNNTTGG 2 cut(s) 4067, 4099
Alw21I GWGCWC 5 cut(s) 291, 764, 868, 2235, 2427
Alw26I GTCTC 7 cut(s) 227, 258, 1246, 2097, 2372, 3060, 3197
Alw44I GTGCAC 1 cut(s) 2231
AlwI GGATC 9 cut(s) 1137, 1434, 1615, 2258, 2459, 2745, 3459, 3727, 3740
AlwNI CAGNNNCTG 3 cut(s) 2516, 3818, 3941
AoxI GGCC 4 cut(s) 401, 695, 1471, 3180
ApaLI GTGCAC 1 cut(s) 2231
ApeKI GCWGC 9 cut(s) 209, 301, 350, 365, 1546, 2111, 2711, 4055, 4070
ApoI RAATTY 7 cut(s) 1820, 1852, 1965, 2169, 2897, 4247, 4331
ArsI GACNNNNNNTTYG 2 cut(s) 4106, 4138
Asp700I GAANNNNTTC 1 cut(s) 2439
AspLEI GCGC 2 cut(s) 316, 3935
AspS9I GGNCC 3 cut(s) 401, 551, 1730
AsuC2I CCSGG 2 cut(s) 2996, 3766
AsuHPI GGTGA 6 cut(s) 229, 448, 709, 1562, 3787, 4231
AsuII TTCGAA 2 cut(s) 1436, 2757
AvaII GGWCC 2 cut(s) 551, 1730
AxyI CCTNAGG 2 cut(s) 2415, 3577
BaeGI GKGCMC 2 cut(s) 2235, 4308
BamHI GGATCC 1 cut(s) 3732
BanI GGYRCC 1 cut(s) 1256
BanII GRGCYC 2 cut(s) 2427, 2568
BarI GAAGNNNNNNTAC 2 cut(s) 875, 907
BbrPI CACGTG 1 cut(s) 3894
BbsI GAAGAC 3 cut(s) 525, 570, 4075
Bbv12I GWGCWC 5 cut(s) 291, 764, 868, 2235, 2427
BbvCI CCTCAGC 1 cut(s) 1030
BbvI GCAGC 9 cut(s) 221, 288, 352, 362, 1558, 2098, 2723, 4057, 4067
BccI CCATC 9 cut(s) 146, 585, 607, 706, 851, 1748, 2710, 2874, 2903
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 7 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629
BciVI GTATCC 4 cut(s) 481, 2309, 2365, 3800
BclI TGATCA 2 cut(s) 1177, 4180
BcnI CCSGG 2 cut(s) 2996, 3766
BcoDI GTCTC 7 cut(s) 227, 258, 1246, 2097, 2372, 3060, 3197
BcuI ACTAGT 1 cut(s) 3721
BfaI CTAG 9 cut(s) 900, 954, 1605, 1794, 2136, 2658, 3132, 3288, 3722
BfmI CTRYAG 1 cut(s) 207
BfrI CTTAAG 2 cut(s) 1900, 1951
BfuAI ACCTGC 1 cut(s) 1741
BfuI GTATCC 4 cut(s) 481, 2309, 2365, 3800
BglI GCCNNNNNGGC 1 cut(s) 2999
BglII AGATCT 3 cut(s) 688, 2694, 3412
Bme1390I CCNGG 9 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629, 2996, 3766
Bme18I GGWCC 2 cut(s) 551, 1730
BmgBI CACGTC 2 cut(s) 670, 1093
BmgT120I GGNCC 3 cut(s) 401, 551, 1730
BmiI GGNNCC 4 cut(s) 1258, 1731, 2914, 3734
BmrFI CCNGG 9 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629, 2996, 3766
BmrI ACTGGG 1 cut(s) 4324
BmsI GCATC 9 cut(s) 1183, 1205, 1905, 3513, 3591, 3608, 3968, 4050, 4338
BmuI ACTGGG 1 cut(s) 4324
BoxI GACNNNNGTC 1 cut(s) 2196
BpiI GAAGAC 3 cut(s) 525, 570, 4075
BplI GAGNNNNNCTC 6 cut(s) 273, 305, 1552, 1584, 2369, 2401
BpmI CTGGAG 3 cut(s) 291, 2607, 4159
Bpu10I CCTNAGC 1 cut(s) 1030
Bpu14I TTCGAA 2 cut(s) 1436, 2757
BpuEI CTTGAG 5 cut(s) 1285, 2060, 2079, 2159, 3179
BpuMI CCSGG 2 cut(s) 2996, 3766
BsaAI YACGTR 3 cut(s) 1519, 3894, 4001
BsaI GGTCTC 4 cut(s) 258, 2097, 3060, 3197
BsaJI CCNNGG 7 cut(s) 248, 483, 2267, 2358, 2468, 2994, 3206
BsaWI WCCGGW 2 cut(s) 214, 3248
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 911, 941, 1535, 1565
Bsc4I CCNNNNNNNGG 8 cut(s) 394, 737, 794, 1725, 2922, 3094, 3955, 4314
Bse1I ACTGG 9 cut(s) 395, 532, 1840, 3056, 3469, 3685, 3862, 4176, 4319
Bse21I CCTNAGG 2 cut(s) 2415, 3577
Bse3DI GCAATG 5 cut(s) 628, 3271, 3526, 3657, 3960
BseBI CCWGG 7 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629
BseDI CCNNGG 7 cut(s) 248, 483, 2267, 2358, 2468, 2994, 3206
BseGI GGATG 7 cut(s) 826, 2222, 2368, 3076, 3245, 3574, 3582
BseLI CCNNNNNNNGG 8 cut(s) 394, 737, 794, 1725, 2922, 3094, 3955, 4314
BseMI GCAATG 5 cut(s) 628, 3271, 3526, 3657, 3960
BseNI ACTGG 9 cut(s) 395, 532, 1840, 3056, 3469, 3685, 3862, 4176, 4319
BseRI GAGGAG 4 cut(s) 2439, 2574, 2577, 4025
BseSI GKGCMC 2 cut(s) 2235, 4308
BseXI GCAGC 9 cut(s) 221, 288, 352, 362, 1558, 2098, 2723, 4057, 4067
BsgI GTGCAG 1 cut(s) 3850
Bsh1236I CGCG 1 cut(s) 636
BshFI GGCC 4 cut(s) 403, 697, 1473, 3182
BshNI GGYRCC 1 cut(s) 1256
BsiHKAI GWGCWC 5 cut(s) 291, 764, 868, 2235, 2427
BsiSI CCGG 4 cut(s) 215, 2996, 3249, 3765
BslFI GGGAC 4 cut(s) 942, 1011, 1316, 1743
BslI CCNNNNNNNGG 8 cut(s) 394, 737, 794, 1725, 2922, 3094, 3955, 4314
BsmAI GTCTC 7 cut(s) 227, 258, 1246, 2097, 2372, 3060, 3197
BsmFI GGGAC 4 cut(s) 942, 1011, 1316, 1743
BsmI GAATGC 4 cut(s) 226, 667, 2285, 3694
BsnI GGCC 4 cut(s) 403, 697, 1473, 3182
Bso31I GGTCTC 4 cut(s) 258, 2097, 3060, 3197
Bsp119I TTCGAA 2 cut(s) 1436, 2757
Bsp1286I GDGCHC 7 cut(s) 291, 764, 868, 2235, 2427, 2568, 4308
Bsp1407I TGTACA 2 cut(s) 144, 3114
BspACI CCGC 3 cut(s) 2109, 2483, 2923
BspANI GGCC 4 cut(s) 403, 697, 1473, 3182
BspFNI CGCG 1 cut(s) 636
BspHI TCATGA 5 cut(s) 174, 657, 1018, 1938, 3136
BspLI GGNNCC 4 cut(s) 1258, 1731, 2914, 3734
BspMAI CTGCAG 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 1741
BspPI GGATC 9 cut(s) 1137, 1434, 1615, 2258, 2459, 2745, 3459, 3727, 3740
BspQI GCTCTTC 2 cut(s) 856, 3893
BspT104I TTCGAA 2 cut(s) 1436, 2757
BspT107I GGYRCC 1 cut(s) 1256
BspTI CTTAAG 2 cut(s) 1900, 1951
BspTNI GGTCTC 4 cut(s) 258, 2097, 3060, 3197
BsrDI GCAATG 5 cut(s) 628, 3271, 3526, 3657, 3960
BsrGI TGTACA 2 cut(s) 144, 3114
BsrI ACTGG 9 cut(s) 395, 532, 1840, 3056, 3469, 3685, 3862, 4176, 4319
BssECI CCNNGG 7 cut(s) 248, 483, 2267, 2358, 2468, 2994, 3206
BssT1I CCWWGG 3 cut(s) 2267, 2468, 3206
Bst2UI CCWGG 7 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629
Bst6I CTCTTC 7 cut(s) 856, 952, 1498, 2459, 3102, 3893, 4024
BstAFI CTTAAG 2 cut(s) 1900, 1951
BstAPI GCANNNNNTGC 1 cut(s) 3965
BstAUI TGTACA 2 cut(s) 144, 3114
BstBAI YACGTR 3 cut(s) 1519, 3894, 4001
BstBI TTCGAA 2 cut(s) 1436, 2757
BstDSI CCRYGG 2 cut(s) 248, 483
BstEII GGTNACC 2 cut(s) 1662, 3881
BstF5I GGATG 7 cut(s) 826, 2222, 2368, 3076, 3245, 3574, 3582
BstFNI CGCG 1 cut(s) 636
BstHHI GCGC 2 cut(s) 316, 3935
BstMAI GTCTC 7 cut(s) 227, 258, 1246, 2097, 2372, 3060, 3197
BstMWI GCNNNNNNNGC 9 cut(s) 1552, 1697, 2136, 2794, 2999, 3380, 3532, 3681, 3965
BstNI CCWGG 7 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629
BstNSI RCATGY 3 cut(s) 1331, 3121, 3972
BstPAI GACNNNNGTC 1 cut(s) 2196
BstPI GGTNACC 2 cut(s) 1662, 3881
BstSCI CCNGG 9 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627, 2994, 3764
BstSFI CTRYAG 1 cut(s) 207
BstSLI GKGCMC 2 cut(s) 2235, 4308
BstSNI TACGTA 1 cut(s) 1519
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 9 cut(s) 221, 288, 352, 362, 1558, 2098, 2723, 4057, 4067
BstV2I GAAGAC 3 cut(s) 525, 570, 4075
BstX2I RGATCY 6 cut(s) 688, 1129, 1620, 2694, 3412, 3732
BstXI CCANNNNNNTGG 3 cut(s) 345, 1126, 3213
BstYI RGATCY 6 cut(s) 688, 1129, 1620, 2694, 3412, 3732
Bsu36I CCTNAGG 2 cut(s) 2415, 3577
BsuI GTATCC 4 cut(s) 481, 2309, 2365, 3800
BsuRI GGCC 4 cut(s) 403, 697, 1473, 3182
BtgI CCRYGG 2 cut(s) 248, 483
BtgZI GCGATG 1 cut(s) 4073
BtrI CACGTC 2 cut(s) 670, 1093
BtsCI GGATG 7 cut(s) 826, 2222, 2368, 3076, 3245, 3574, 3582
BtsI GCAGTG 2 cut(s) 454, 1119
BveI ACCTGC 1 cut(s) 1741
CaiI CAGNNNCTG 3 cut(s) 2516, 3818, 3941
CciI TCATGA 5 cut(s) 174, 657, 1018, 1938, 3136
CfoI GCGC 2 cut(s) 316, 3935
Cfr13I GGNCC 3 cut(s) 401, 551, 1730
CsiI ACCWGGT 1 cut(s) 1658
DraI TTTAAA 3 cut(s) 504, 2155, 3256
DraIII CACNNNGTG 2 cut(s) 772, 1414
Eam1104I CTCTTC 7 cut(s) 856, 952, 1498, 2459, 3102, 3893, 4024
EarI CTCTTC 7 cut(s) 856, 952, 1498, 2459, 3102, 3893, 4024
Ecl136II GAGCTC 1 cut(s) 2425
Eco105I TACGTA 1 cut(s) 1519
Eco130I CCWWGG 3 cut(s) 2267, 2468, 3206
Eco24I GRGCYC 2 cut(s) 2427, 2568
Eco31I GGTCTC 4 cut(s) 258, 2097, 3060, 3197
Eco32I GATATC 2 cut(s) 92, 2663
Eco47I GGWCC 2 cut(s) 551, 1730
Eco53kI GAGCTC 1 cut(s) 2425
Eco57I CTGAAG 2 cut(s) 534, 2828
Eco72I CACGTG 1 cut(s) 3894
Eco81I CCTNAGG 2 cut(s) 2415, 3577
Eco91I GGTNACC 2 cut(s) 1662, 3881
EcoICRI GAGCTC 1 cut(s) 2425
EcoO109I RGGNCCY 1 cut(s) 1730
EcoO65I GGTNACC 2 cut(s) 1662, 3881
EcoRII CCWGG 7 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627
EcoRV GATATC 2 cut(s) 92, 2663
EcoT14I CCWWGG 3 cut(s) 2267, 2468, 3206
EcoT22I ATGCAT 6 cut(s) 1198, 1329, 3528, 3601, 3696, 4065
EcoT38I GRGCYC 2 cut(s) 2427, 2568
ErhI CCWWGG 3 cut(s) 2267, 2468, 3206
FalI AAGNNNNNCTT 4 cut(s) 2058, 2090, 2587, 2619
FaqI GGGAC 4 cut(s) 942, 1011, 1316, 1743
FauNDI CATATG 1 cut(s) 2431
FbaI TGATCA 2 cut(s) 1177, 4180
FblI GTMKAC 3 cut(s) 1161, 3761, 4128
FokI GGATG 7 cut(s) 813, 2229, 2375, 3083, 3232, 3569, 3581
FriOI GRGCYC 2 cut(s) 2427, 2568
FspBI CTAG 9 cut(s) 900, 954, 1605, 1794, 2136, 2658, 3132, 3288, 3722
GlaI GCGC 2 cut(s) 315, 3934
GsuI CTGGAG 3 cut(s) 291, 2607, 4159
HaeIII GGCC 4 cut(s) 403, 697, 1473, 3182
HapII CCGG 4 cut(s) 215, 2996, 3249, 3765
HhaI GCGC 2 cut(s) 316, 3935
Hin6I GCGC 2 cut(s) 314, 3933
HinP1I GCGC 2 cut(s) 314, 3933
HincII GTYRAC 4 cut(s) 1162, 1486, 2478, 4114
HindII GTYRAC 4 cut(s) 1162, 1486, 2478, 4114
HindIII AAGCTT 3 cut(s) 1698, 1833, 3170
HpaII CCGG 4 cut(s) 215, 2996, 3249, 3765
HphI GGTGA 6 cut(s) 229, 448, 709, 1562, 3787, 4231
HpyCH4IV ACGT 7 cut(s) 669, 1092, 1518, 3492, 3834, 3893, 4000
HpyF10VI GCNNNNNNNGC 9 cut(s) 1552, 1697, 2136, 2794, 2999, 3380, 3532, 3681, 3965
HpySE526I ACGT 7 cut(s) 669, 1092, 1518, 3492, 3834, 3893, 4000
HspAI GCGC 2 cut(s) 314, 3933
Ksp22I TGATCA 2 cut(s) 1177, 4180
LguI GCTCTTC 2 cut(s) 856, 3893
LmnI GCTCC 8 cut(s) 127, 217, 1169, 1543, 2422, 2518, 2835, 4178
Lsp1109I GCAGC 9 cut(s) 221, 288, 352, 362, 1558, 2098, 2723, 4057, 4067
LweI GCATC 9 cut(s) 1183, 1205, 1905, 3513, 3591, 3608, 3968, 4050, 4338
MabI ACCWGGT 1 cut(s) 1658
MaeI CTAG 9 cut(s) 900, 954, 1605, 1794, 2136, 2658, 3132, 3288, 3722
MaeII ACGT 7 cut(s) 669, 1092, 1518, 3492, 3834, 3893, 4000
MfeI CAATTG 2 cut(s) 1376, 1384
MflI RGATCY 6 cut(s) 688, 1129, 1620, 2694, 3412, 3732
MhlI GDGCHC 7 cut(s) 291, 764, 868, 2235, 2427, 2568, 4308
MlyI GAGTC 6 cut(s) 172, 268, 1157, 1754, 3417, 3809
MmeI TCCRAC 3 cut(s) 775, 1743, 3485
Mph1103I ATGCAT 6 cut(s) 1198, 1329, 3528, 3601, 3696, 4065
MroXI GAANNNNTTC 1 cut(s) 2439
MslI CAYNNNNRTG 4 cut(s) 1322, 2205, 2406, 2907
MspA1I CMGCKG 4 cut(s) 353, 1549, 2111, 2923
MspCI CTTAAG 2 cut(s) 1900, 1951
MspI CCGG 4 cut(s) 215, 2996, 3249, 3765
MspR9I CCNGG 9 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629, 2996, 3766
MunI CAATTG 2 cut(s) 1376, 1384
Mva1269I GAATGC 4 cut(s) 226, 667, 2285, 3694
MvaI CCWGG 7 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 9 cut(s) 1552, 1697, 2136, 2794, 2999, 3380, 3532, 3681, 3965
NciI CCSGG 2 cut(s) 2996, 3766
NdeI CATATG 1 cut(s) 2431
NlaIV GGNNCC 4 cut(s) 1258, 1731, 2914, 3734
NmeAIII GCCGAG 1 cut(s) 1020
NmuCI GTSAC 5 cut(s) 1080, 1640, 3343, 3889, 4043
NsiI ATGCAT 6 cut(s) 1198, 1329, 3528, 3601, 3696, 4065
NspI RCATGY 3 cut(s) 1331, 3121, 3972
NspV TTCGAA 2 cut(s) 1436, 2757
PaeI GCATGC 1 cut(s) 3972
PagI TCATGA 5 cut(s) 174, 657, 1018, 1938, 3136
PciSI GCTCTTC 2 cut(s) 856, 3893
PcsI WCGNNNNNNNCGW 1 cut(s) 1442
PctI GAATGC 4 cut(s) 226, 667, 2285, 3694
PdmI GAANNNNTTC 1 cut(s) 2439
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 3 cut(s) 394, 794, 4314
PfoI TCCNGGA 1 cut(s) 1422
PleI GAGTC 6 cut(s) 172, 267, 1157, 1753, 3417, 3809
PmaCI CACGTG 1 cut(s) 3894
PmlI CACGTG 1 cut(s) 3894
PpsI GAGTC 6 cut(s) 172, 267, 1157, 1753, 3417, 3809
Ppu21I YACGTR 3 cut(s) 1519, 3894, 4001
PpuMI RGGWCCY 1 cut(s) 1730
PshAI GACNNNNGTC 1 cut(s) 2196
Psp124BI GAGCTC 1 cut(s) 2427
Psp5II RGGWCCY 1 cut(s) 1730
Psp6I CCWGG 7 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627
PspCI CACGTG 1 cut(s) 3894
PspEI GGTNACC 2 cut(s) 1662, 3881
PspGI CCWGG 7 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627
PspN4I GGNNCC 4 cut(s) 1258, 1731, 2914, 3734
PspPI GGNCC 3 cut(s) 401, 551, 1730
PspPPI RGGWCCY 1 cut(s) 1730
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 3 cut(s) 2516, 3818, 3941
PsuI RGATCY 6 cut(s) 688, 1129, 1620, 2694, 3412, 3732
PsyI GACNNNGTC 1 cut(s) 671
PvuII CAGCTG 2 cut(s) 353, 1549
RseI CAYNNNNRTG 4 cut(s) 1322, 2205, 2406, 2907
SacI GAGCTC 1 cut(s) 2427
SalI GTCGAC 1 cut(s) 1160
SapI GCTCTTC 2 cut(s) 856, 3893
Sau96I GGNCC 3 cut(s) 401, 551, 1730
SchI GAGTC 6 cut(s) 172, 268, 1157, 1754, 3417, 3809
ScrFI CCNGG 9 cut(s) 309, 789, 1098, 1424, 1660, 2359, 2629, 2996, 3766
SduI GDGCHC 7 cut(s) 291, 764, 868, 2235, 2427, 2568, 4308
SexAI ACCWGGT 1 cut(s) 1658
SfaNI GCATC 9 cut(s) 1183, 1205, 1905, 3513, 3591, 3608, 3968, 4050, 4338
SfcI CTRYAG 1 cut(s) 207
SfuI TTCGAA 2 cut(s) 1436, 2757
SinI GGWCC 2 cut(s) 551, 1730
SmiMI CAYNNNNRTG 4 cut(s) 1322, 2205, 2406, 2907
SmlI CTYRAG 7 cut(s) 1300, 1900, 1951, 2039, 2094, 2174, 3194
SmoI CTYRAG 7 cut(s) 1300, 1900, 1951, 2039, 2094, 2174, 3194
SnaBI TACGTA 1 cut(s) 1519
SpeI ACTAGT 1 cut(s) 3721
SphI GCATGC 1 cut(s) 3972
SsiI CCGC 3 cut(s) 2109, 2483, 2923
SspI AATATT 1 cut(s) 1891
SspMI CTAG 9 cut(s) 900, 954, 1605, 1794, 2136, 2658, 3132, 3288, 3722
SstI GAGCTC 1 cut(s) 2427
StyD4I CCNGG 9 cut(s) 307, 787, 1096, 1422, 1658, 2357, 2627, 2994, 3764
StyI CCWWGG 3 cut(s) 2267, 2468, 3206
TaiI ACGT 7 cut(s) 672, 1095, 1521, 3495, 3837, 3896, 4003
TaqII GACCGA 1 cut(s) 3915
TatI WGTACW 4 cut(s) 144, 1476, 1923, 3114
TauI GCSGC 1 cut(s) 2926
TseFI GTSAC 5 cut(s) 1080, 1640, 3343, 3889, 4043
TseI GCWGC 9 cut(s) 209, 301, 350, 365, 1546, 2111, 2711, 4055, 4070
Tsp45I GTSAC 5 cut(s) 1080, 1640, 3343, 3889, 4043
TspGWI ACGGA 6 cut(s) 73, 500, 1234, 2195, 3801, 3904
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 3 cut(s) 394, 794, 4314
Vha464I CTTAAG 2 cut(s) 1900, 1951
VneI GTGCAC 1 cut(s) 2231
VpaK11BI GGWCC 2 cut(s) 551, 1730
XapI RAATTY 7 cut(s) 1820, 1852, 1965, 2169, 2897, 4247, 4331
XbaI TCTAGA 1 cut(s) 1793
XceI RCATGY 3 cut(s) 1331, 3121, 3972
XcmI CCANNNNNNNNNTGG 3 cut(s) 395, 610, 2085
XmiI GTMKAC 3 cut(s) 1161, 3761, 4128
XmnI GAANNNNTTC 1 cut(s) 2439
XspI CTAG 9 cut(s) 900, 954, 1605, 1794, 2136, 2658, 3132, 3288, 3722
Zsp2I ATGCAT 6 cut(s) 1198, 1329, 3528, 3601, 3696, 4065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.