MD10G1307000.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000071
Physical Location & Seq
Reverse (-)
39251161 .. 39255577
4417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1307000.v1.1.491

Sequence Viewer

Length: 3636 bp
ATGTCATCAGTCATCATTGAAATGAGTCTTGCCCTAGGTGGAAGATCCAAGCTAGGGTTCATAAATGGAAGCATCGAAGTCCCAGATGTTTCCTCACCAACTTATGAAATCTGGCTTTGCAAGGATCAGCTAGTTATGTCATGGCTCCTAAATTCCATGGAACGTAAACTAGCTGAAATATTCAGTTATTCTGAATCATCATTCAAGCTATGGGAGACAGTCAAGGAGATGTATGGCAGCCAGAACAATGCTGCACGTGTGTTTCAATTGAAGAAGGACATTTCCAACCTACAACAAGAAGGAAAGCCCTTTGTTCAACTTCTGGGCAGCATGAAGAGCATGTGGAATGAGCTGGAAATGTATCGGCCTCATACAACTGAGGCGTCTTTGCTACTAAAAAGGGCAGAAGAAGACAAGATATTCCAACTCTTGTCAAGCCTCGATTCAGGATACGAAGACCTACGAAGCCACATACTCATGAACCCCGATCTACCTTCCTTCACCAGCGTATGTGCAACAATCCAACGTGAAGAAGTACGAAGGAAAGTCATGAACATTGGCACAAAGACCGGTGTAACTGAAGCAAGGGCTTATCTAGCCAACGAAAGAAAATACAAAGTGAAGAATCCATACTTAAAGTGTCAACACTGCAACTATACTGGTCACGTTAAGGAGACATGCTGGACTTTACATCCAGAATTAAAGCCAGAGTTCATGAAGGATAACAAGGGCTCACAAAGACTGAACCGTGCACCACACAGAGCCAACAATGCAACTGCCTCAACATCTCATGGTTCTGATGCACTCAAGAATTTCACTGCAAATCCAGCTGCACTCATAAATGAGTTTGCAGTGTATCTTCAAAGCAAGAAAGAGGTTGGCTTTGAAGATGGAAATTCAATAGCTTTGCTAGGCAAGTTCGCTGGATTTCTGACAGACACACAACACATGACCCGAGATGACATGCAAGGTATCATGACTGCTTTCAAAACTGCTCTTAATGTAAATATGATGCATGATTTGTGGATTGTTGATTCGGGTGCCACAGATCATATGACCAACCATGTTTCTAAGTTTCACAAGTTTGAAAAATTTTTCAAAAACCTTCTCAAGTCTCAACTGCCAATGGATTGCCTCACCAAGAAGACGATTGGTGAAGGGTTTTACCTTGATGGCCTCTATTACATATCAAAAAGCAGTCCCAGAGGATTTCAAGCCAAGTCCAGTCCATCCCAAGATAGTCAATTGTGGCATCAACGTTTAGCTCATCCTGCCCAACCTGTTTTGTCATCCTTGTTTCCAAACTTAGGAAATGATTTAATTTTGTGTGAAACATGTCATTTGTCTAAAGCCACCAGATTACCTTTTACCTCATCCTTATCTAGGACTAGTAAAGTTTTCGAACTAGTTCACTCAGATGTATGGGGACCAACGTTTGAATCTTTTGATGGTTATAAGTATTATGTAACGTTTGTTGATGATTTCTCTAGAGCCACCTGGTTATACCTTTTAAAGTCTAAGAGTGAAGTGATTGAAGTTTTTAAGGATTTTCACAATCTTGTTAAGAATCATTTTTCCTCTCAAATTCAAACCTTAAGATCTGACAATGGCACCGAATATATGTCCCATATCATGTCATTATATTTGAGCAAGCACGGTATCATGCATCAAACAAGTTGTGTCGGCACACCTCAACAAAATGGTGTAGCTGAAAGAAAAAATCGTGACATACTGGAAAAAACGAGAGCATTAATGTTACAAATGAACGTACCAAAAAGATTTTGGTCACATGGAGTCATGGCGGCTGTGTACATCATCAATAGACTTCCTAGCCGAGTCTTGGGGTTTAAATCTCCACTTGAAGTCATGAAAGGAAGAAAAATCGACCTTTCACATCTTAGAGTCTTTGGTTGTGTCTGTTTTGTTCATATACAGTCACACCATCGAGATAAATTGGACCCTAGAGCTCTCAAATGTATTTTTCTTGGTTATTCATCAACACAAAAAGGATATAAGTGCTATGATCCTCAACTAAGAAAAATAATTGTATCTAAAGATGTTCGATTTCATGAAGCTAACCCTTTTTTCAGCAAATCACATGAAGCCACAAGTCAGGGGGAGTGCATCTTAGACCTAATTCCTTTACCAAGAATCTGTACTCAAGATGTTTTAAATGACAGAGGTGCTAACCATGACCACATTGATGAATCTTCTACACCTCTCATTGAAGTCAATAATGAAGGTACTCATCCTGATAGTTCTGTGCATGATGATGACAACGGCAACCAAGAAAATCCTCAAGGTGTGGATGAAAATTTGAACGAGGATGAGATAAACCTCCCAGAACCTCGACACAATCCTCGGCGTGATAGACAACCTCCCACAAGGTTTCAAGATTTTGTCACATATAAACCGAGGCATCCAATTTCAAATTGCGTGTCGTATCAAAAGGTAACTCCATCTCATGCTGCCTTTCTTAACAATATTTCAAGTCACAGTGAGCCTCAAAACTTTTATGAGGCTAAAAACCAAGTTGTGTGGAAAGAAGCAATGAGAGATGAACTCAAAGCACTAGACCAACACAAAACCTGGAGCATCACCAAACTTCCACAAGGAAAAAAGGCAGTGGGCTGCAAGTGGATCTATAAGATCAAGTTTAATTCTGATGGGTCGATTGAGAGGCATAAAGCCAGGCTGGTGGCTCGAGGATTCACTCAAACATTTGAGGTGGATTACAAGGAAACATTTGCTCCTGTTGCAAAAATGAACTCAGTAAGGGTTCTATTGTCTGTTGCGGTCAATAAAGGTTGGTCTATGTACCAAATGGACGTGAAGAACGCCTTTCTACATGGTGATCTTGAAGAAGAAGTCTATATGAGATTGCCACCTGGACACCCTCAAAGCCAAGAGCCTAATTTGGTGTGTAAGTTACATAAGTCAATTTATGGATTGAAGCAATCGCCACGTGCTTGGTATGCAAAACTAAGTACCGTTCTTCATAGCATTGGTTTCAAAAGGAGTAATGCAGATTCATCATTATTTGTTCGCACAAGAGCTGCGAGCAAATTAGTTGTGTTAATATATGTGGATGACTTAATAATTACAGGTGATAATGCTGCTGAAATCACCACACTCAAACAGTCACTTCAACAAAAATTTGCAGTCAAAGATCTTGGGGTGTTGAAGTATTTCCTTGGAATCGAAATGGCATCTTCTTACAAGGGACTATTTCTTAATCAACGAAAATACGTCATGGACCTACTTAAAGATGCTAATATGAGTGATGCTAAGCCTGCACTTACCCCTCTTGATAGCAAGCTCAAGCTTGACTTGGGAGGCACGTCACTCTCGGATATCAGCTTATATCAGAGGCTTGTCGGCAAGCTGATCTACCTAACGATCACCAGACCAAACATCTCACATTCAGTAAGTATCGCCAGTCAGTTCATGCACTCTCCCACCATCGAGCATCTGAACCTTGTCAAAAGGATCTTACGTTACTTAAAAGGGTCTGTTGGTCGTGGCATCCTCATGGCAAAGAATGACAACACTCAAATCATGGGAAACAAACTGTCATCGCAAGATCAAGTGCTGAAGCTGAATATCGTGCAATGGCCTCAACCGCGTGTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1212

Amino Acids

137.82

Weight (kDa)

9.15

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 13 - 183 1.7e-11 gag-polypeptide of LTR copia-type
gag_pre-integrs PF13976 392 - 450 2.7e-08 GAG-pre-integrase domain
rve PF00665 466 - 564 3.7e-12 Integrase core domain
SH3_retrovirus PF25597 638 - 700 7.4e-28 Retroviral polymerase SH3-like domain
RVT_2 PF07727 862 - 1103 5.1e-79 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1455
AccB1I GGYRCC 2 cut(s) 1040, 1610
AccII CGCG 1 cut(s) 3625
AciI CCGC 3 cut(s) 1802, 2795, 3623
AclI AACGTT 3 cut(s) 1258, 1433, 1469
AclWI GGATC 5 cut(s) 39, 132, 2018, 2648, 3497
AcsI RAATTY 7 cut(s) 151, 811, 895, 1091, 1584, 2314, 3155
AcuI CTGAAG 2 cut(s) 600, 3614
AcvI CACGTG 2 cut(s) 257, 2966
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 7 cut(s) 537, 1770, 1811, 2158, 2245, 2819, 2989
AfiI CCNNNNNNNGG 4 cut(s) 54, 1307, 1383, 1840
AflII CTTAAG 1 cut(s) 1594
AflIII ACRYGT 2 cut(s) 256, 1334
AgeI ACCGGT 1 cut(s) 569
AhlI ACTAGT 2 cut(s) 1388, 1405
AjiI CACGTC 2 cut(s) 2830, 3342
AjnI CCWGG 4 cut(s) 1496, 2588, 2690, 2887
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
Alw21I GWGCWC 2 cut(s) 754, 1969
Alw26I GTCTC 3 cut(s) 209, 668, 1119
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 5 cut(s) 39, 132, 2018, 2648, 3497
Ama87I CYCGRG 2 cut(s) 954, 2703
AoxI GGCC 3 cut(s) 365, 1174, 3614
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 8 cut(s) 237, 251, 327, 830, 2468, 2631, 3056, 3116
ApoI RAATTY 7 cut(s) 151, 811, 895, 1091, 1584, 2314, 3155
ArsI GACNNNNNNTTYG 2 cut(s) 2423, 2455
AseI ATTAAT 1 cut(s) 1751
AsiGI ACCGGT 1 cut(s) 569
Asp700I GAANNNNTTC 2 cut(s) 1407, 3188
AspA2I CCTAGG 1 cut(s) 34
AspS9I GGNCC 3 cut(s) 1427, 1957, 3256
AsuHPI GGTGA 9 cut(s) 87, 493, 1129, 1166, 2590, 2864, 3118, 3119, 3394
AsuII TTCGAA 1 cut(s) 1401
AvaI CYCGRG 2 cut(s) 954, 2703
AvaII GGWCC 3 cut(s) 1427, 1957, 3256
AvrII CCTAGG 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 754
BanI GGYRCC 2 cut(s) 1040, 1610
BanII GRGCYC 2 cut(s) 734, 1969
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BbrPI CACGTG 2 cut(s) 257, 2966
BbsI GAAGAC 3 cut(s) 417, 462, 1151
Bbv12I GWGCWC 2 cut(s) 754, 1969
BbvI GCAGC 8 cut(s) 238, 249, 339, 817, 2455, 2618, 3043, 3103
BccI CCATC 8 cut(s) 884, 1166, 1237, 1442, 1950, 2467, 2660, 3470
BceAI ACGGC 1 cut(s) 2296
BciT130I CCWGG 4 cut(s) 1498, 2590, 2692, 2889
BciVI GTATCC 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 209, 668, 1119
BcuI ACTAGT 2 cut(s) 1388, 1405
BfrI CTTAAG 1 cut(s) 1594
BfuI GTATCC 1 cut(s) 443
BglII AGATCT 2 cut(s) 1598, 3169
BisI GCNGC 9 cut(s) 238, 252, 328, 831, 1803, 2469, 2632, 3057, 3117
BlnI CCTAGG 1 cut(s) 34
BlpI GCTNAGC 1 cut(s) 3288
BlsI GCNGC 9 cut(s) 239, 253, 329, 832, 1804, 2470, 2633, 3058, 3118
Bme1390I CCNGG 4 cut(s) 1498, 2590, 2692, 2889
Bme18I GGWCC 3 cut(s) 1427, 1957, 3256
BmeT110I CYCGRG 2 cut(s) 954, 2703
BmgBI CACGTC 2 cut(s) 2830, 3342
BmgT120I GGNCC 3 cut(s) 1427, 1957, 3256
BmiI GGNNCC 5 cut(s) 146, 1042, 1428, 1612, 1959
BmrFI CCNGG 4 cut(s) 1498, 2590, 2692, 2889
BpiI GAAGAC 3 cut(s) 417, 462, 1151
BplI GAGNNNNNCTC 2 cut(s) 2547, 2579
BpmI CTGGAG 1 cut(s) 2611
Bpu1102I GCTNAGC 1 cut(s) 3288
Bpu14I TTCGAA 1 cut(s) 1401
BpuEI CTTGAG 5 cut(s) 791, 1094, 2145, 2283, 3305
BsaAI YACGTR 2 cut(s) 257, 2966
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 5 cut(s) 34, 156, 2360, 2414, 3193
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 2734, 2764
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 1307, 1383, 1840
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 4 cut(s) 664, 1224, 1737, 3438
Bse3DI GCAATG 2 cut(s) 2556, 3617
BseBI CCWGG 4 cut(s) 1498, 2590, 2692, 2889
BseDI CCNNGG 5 cut(s) 34, 156, 2360, 2414, 3193
BseLI CCNNNNNNNGG 4 cut(s) 54, 1307, 1383, 1840
BseMI GCAATG 2 cut(s) 2556, 3617
BseMII CTCAG 3 cut(s) 369, 1428, 2784
BseNI ACTGG 4 cut(s) 664, 1224, 1737, 3438
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 8 cut(s) 238, 249, 339, 817, 2455, 2618, 3043, 3103
BsgI GTGCAG 3 cut(s) 237, 816, 3279
Bsh1236I CGCG 1 cut(s) 3625
BshFI GGCC 3 cut(s) 367, 1176, 3616
BshNI GGYRCC 2 cut(s) 1040, 1610
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 754, 1969
BsiHKCI CYCGRG 2 cut(s) 954, 2703
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 5 cut(s) 65, 1185, 1440, 1609, 3237
BslI CCNNNNNNNGG 4 cut(s) 54, 1307, 1383, 1840
BsmAI GTCTC 3 cut(s) 209, 668, 1119
BsmFI GGGAC 5 cut(s) 65, 1185, 1440, 1609, 3237
BsnI GGCC 3 cut(s) 367, 1176, 3616
BsoBI CYCGRG 2 cut(s) 954, 2703
Bsp119I TTCGAA 1 cut(s) 1401
Bsp1286I GDGCHC 3 cut(s) 734, 754, 1969
Bsp1407I TGTACA 1 cut(s) 1809
Bsp1720I GCTNAGC 1 cut(s) 3288
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 3 cut(s) 1802, 2795, 3623
BspANI GGCC 3 cut(s) 367, 1176, 3616
BspCNI CTCAG 3 cut(s) 370, 1427, 2783
BspFNI CGCG 1 cut(s) 3625
BspHI TCATGA 6 cut(s) 477, 549, 714, 975, 1866, 2068
BspLI GGNNCC 5 cut(s) 146, 1042, 1428, 1612, 1959
BspPI GGATC 5 cut(s) 39, 132, 2018, 2648, 3497
BspQI GCTCTTC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 1401
BspT107I GGYRCC 2 cut(s) 1040, 1610
BspTI CTTAAG 1 cut(s) 1594
BsrDI GCAATG 2 cut(s) 2556, 3617
BsrFI RCCGGY 1 cut(s) 569
BsrGI TGTACA 1 cut(s) 1809
BsrI ACTGG 4 cut(s) 664, 1224, 1737, 3438
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 5 cut(s) 34, 156, 2360, 2414, 3193
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 3 cut(s) 34, 156, 3193
Bst2UI CCWGG 4 cut(s) 1498, 2590, 2692, 2889
Bst4CI ACNGT 8 cut(s) 220, 749, 1658, 1935, 2498, 2992, 3141, 3573
Bst6I CTCTTC 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 383
BstAFI CTTAAG 1 cut(s) 1594
BstAUI TGTACA 1 cut(s) 1809
BstBAI YACGTR 2 cut(s) 257, 2966
BstBI TTCGAA 1 cut(s) 1401
BstC8I GCNNGC 5 cut(s) 1652, 3061, 3294, 3317, 3383
BstDSI CCRYGG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 1381
BstFNI CGCG 1 cut(s) 3625
BstMAI GTCTC 3 cut(s) 209, 668, 1119
BstMWI GCNNNNNNNGC 9 cut(s) 336, 596, 770, 827, 1271, 2756, 2975, 3293, 3622
BstNI CCWGG 4 cut(s) 1498, 2590, 2692, 2889
BstNSI RCATGY 4 cut(s) 343, 681, 967, 1338
BstSCI CCNGG 4 cut(s) 1496, 2588, 2690, 2887
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 1 cut(s) 3625
BstV1I GCAGC 8 cut(s) 238, 249, 339, 817, 2455, 2618, 3043, 3103
BstV2I GAAGAC 3 cut(s) 417, 462, 1151
BstX2I RGATCY 5 cut(s) 44, 1598, 2640, 3169, 3489
BstXI CCANNNNNNTGG 2 cut(s) 2698, 2970
BstYI RGATCY 5 cut(s) 44, 1598, 2640, 3169, 3489
BsuI GTATCC 1 cut(s) 443
BsuRI GGCC 3 cut(s) 367, 1176, 3616
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 3561
BtrI CACGTC 2 cut(s) 2830, 3342
BtsI GCAGTG 4 cut(s) 646, 816, 858, 2631
BtsIMutI CAGTG 5 cut(s) 646, 816, 858, 2503, 2631
Cac8I GCNNGC 5 cut(s) 1652, 3061, 3294, 3317, 3383
CciI TCATGA 6 cut(s) 477, 549, 714, 975, 1866, 2068
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 1427, 1957, 3256
CseI GACGC 1 cut(s) 372
CsiI ACCWGGT 1 cut(s) 1496
Csp6I GTAC 7 cut(s) 536, 1769, 1810, 2157, 2244, 2818, 2988
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 2519, 2554
CviQI GTAC 7 cut(s) 536, 1769, 1810, 2157, 2244, 2818, 2988
DraI TTTAAA 3 cut(s) 1512, 1849, 2172
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 1967
Eco130I CCWWGG 3 cut(s) 34, 156, 3193
Eco24I GRGCYC 2 cut(s) 734, 1969
Eco32I GATATC 1 cut(s) 3355
Eco47I GGWCC 3 cut(s) 1427, 1957, 3256
Eco53kI GAGCTC 1 cut(s) 1967
Eco57I CTGAAG 2 cut(s) 600, 3614
Eco72I CACGTG 2 cut(s) 257, 2966
Eco88I CYCGRG 2 cut(s) 954, 2703
EcoICRI GAGCTC 1 cut(s) 1967
EcoNI CCTNNNNNAGG 1 cut(s) 1381
EcoRII CCWGG 4 cut(s) 1496, 2588, 2690, 2887
EcoRV GATATC 1 cut(s) 3355
EcoT14I CCWWGG 3 cut(s) 34, 156, 3193
EcoT22I ATGCAT 2 cut(s) 1017, 1668
EcoT38I GRGCYC 2 cut(s) 734, 1969
ErhI CCWWGG 3 cut(s) 34, 156, 3193
FalI AAGNNNNNCTT 4 cut(s) 2825, 2857, 3314, 3346
FaqI GGGAC 5 cut(s) 65, 1185, 1440, 1609, 3237
FauNDI CATATG 1 cut(s) 1053
Fnu4HI GCNGC 9 cut(s) 238, 252, 328, 831, 1803, 2469, 2632, 3057, 3117
FriOI GRGCYC 2 cut(s) 734, 1969
Fsp4HI GCNGC 9 cut(s) 238, 252, 328, 831, 1803, 2469, 2632, 3057, 3117
GluI GCNGC 9 cut(s) 238, 252, 328, 831, 1803, 2469, 2632, 3057, 3117
GsuI CTGGAG 1 cut(s) 2611
HaeIII GGCC 3 cut(s) 367, 1176, 3616
HapII CCGG 1 cut(s) 570
HgaI GACGC 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 644
HindII GTYRAC 1 cut(s) 644
HindIII AAGCTT 1 cut(s) 3323
HpaII CCGG 1 cut(s) 570
HphI GGTGA 9 cut(s) 87, 493, 1129, 1166, 2590, 2864, 3118, 3119, 3394
Hpy166II GTNNAC 5 cut(s) 167, 644, 752, 1411, 1810
Hpy188I TCNGA 9 cut(s) 193, 799, 933, 1417, 1603, 2665, 3352, 3369, 3474
Hpy8I GTNNAC 5 cut(s) 167, 644, 752, 1411, 1810
HpyAV CCTTC 9 cut(s) 268, 293, 504, 508, 534, 712, 1115, 1151, 2234
HpyCH4III ACNGT 8 cut(s) 220, 749, 1658, 1935, 2498, 2992, 3141, 3573
HpyF10VI GCNNNNNNNGC 9 cut(s) 336, 596, 770, 827, 1271, 2756, 2975, 3293, 3622
Hsp92I GRCGYC 1 cut(s) 383
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 3 cut(s) 150, 2592, 2755
Lsp1109I GCAGC 8 cut(s) 238, 249, 339, 817, 2455, 2618, 3043, 3103
MabI ACCWGGT 1 cut(s) 1496
MfeI CAATTG 2 cut(s) 266, 1244
MflI RGATCY 5 cut(s) 44, 1598, 2640, 3169, 3489
MhlI GDGCHC 3 cut(s) 734, 754, 1969
MlyI GAGTC 4 cut(s) 34, 1803, 1845, 1911
MmeI TCCRAC 3 cut(s) 309, 448, 547
Mph1103I ATGCAT 2 cut(s) 1017, 1668
MroXI GAANNNNTTC 2 cut(s) 1407, 3188
MslI CAYNNNNRTG 6 cut(s) 20, 476, 1416, 2202, 2271, 3530
MspA1I CMGCKG 1 cut(s) 830
MspCI CTTAAG 1 cut(s) 1594
MspI CCGG 1 cut(s) 570
MspR9I CCNGG 4 cut(s) 1498, 2590, 2692, 2889
MunI CAATTG 2 cut(s) 266, 1244
MvaI CCWGG 4 cut(s) 1498, 2590, 2692, 2889
MvnI CGCG 1 cut(s) 3625
MwoI GCNNNNNNNGC 9 cut(s) 336, 596, 770, 827, 1271, 2756, 2975, 3293, 3622
NcoI CCATGG 1 cut(s) 156
NdeI CATATG 1 cut(s) 1053
NlaIV GGNNCC 5 cut(s) 146, 1042, 1428, 1612, 1959
NmeAIII GCCGAG 2 cut(s) 1859, 2341
NmuCI GTSAC 8 cut(s) 662, 1724, 1785, 1935, 2401, 2492, 3141, 3342
NsiI ATGCAT 2 cut(s) 1017, 1668
NspI RCATGY 4 cut(s) 343, 681, 967, 1338
NspV TTCGAA 1 cut(s) 1401
PaeR7I CTCGAG 1 cut(s) 2703
PagI TCATGA 6 cut(s) 477, 549, 714, 975, 1866, 2068
PciI ACATGT 1 cut(s) 1334
PciSI GCTCTTC 1 cut(s) 329
PdmI GAANNNNTTC 2 cut(s) 1407, 3188
PinAI ACCGGT 1 cut(s) 569
PkrI GCNGC 9 cut(s) 239, 253, 329, 832, 1804, 2470, 2633, 3058, 3118
PleI GAGTC 4 cut(s) 33, 1802, 1844, 1910
PmaCI CACGTG 2 cut(s) 257, 2966
PmlI CACGTG 2 cut(s) 257, 2966
PpsI GAGTC 4 cut(s) 33, 1802, 1844, 1910
Ppu21I YACGTR 2 cut(s) 257, 2966
PscI ACATGT 1 cut(s) 1334
PshBI ATTAAT 1 cut(s) 1751
PsiI TTATAA 1 cut(s) 1455
Psp124BI GAGCTC 1 cut(s) 1969
Psp1406I AACGTT 3 cut(s) 1258, 1433, 1469
Psp6I CCWGG 4 cut(s) 1496, 2588, 2690, 2887
PspCI CACGTG 2 cut(s) 257, 2966
PspGI CCWGG 4 cut(s) 1496, 2588, 2690, 2887
PspN4I GGNNCC 5 cut(s) 146, 1042, 1428, 1612, 1959
PspPI GGNCC 3 cut(s) 1427, 1957, 3256
PspXI VCTCGAGB 1 cut(s) 2703
PsuI RGATCY 5 cut(s) 44, 1598, 2640, 3169, 3489
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 7 cut(s) 537, 1770, 1811, 2158, 2245, 2819, 2989
RsaNI GTAC 7 cut(s) 536, 1769, 1810, 2157, 2244, 2818, 2988
RseI CAYNNNNRTG 6 cut(s) 20, 476, 1416, 2202, 2271, 3530
SacI GAGCTC 1 cut(s) 1969
SapI GCTCTTC 1 cut(s) 329
SatI GCNGC 9 cut(s) 238, 252, 328, 831, 1803, 2469, 2632, 3057, 3117
Sau96I GGNCC 3 cut(s) 1427, 1957, 3256
SchI GAGTC 4 cut(s) 34, 1803, 1845, 1911
ScrFI CCNGG 4 cut(s) 1498, 2590, 2692, 2889
SduI GDGCHC 3 cut(s) 734, 754, 1969
SexAI ACCWGGT 1 cut(s) 1496
Sfr274I CTCGAG 1 cut(s) 2703
SfuI TTCGAA 1 cut(s) 1401
SinI GGWCC 3 cut(s) 1427, 1957, 3256
SlaI CTCGAG 1 cut(s) 2703
SmiMI CAYNNNNRTG 6 cut(s) 20, 476, 1416, 2202, 2271, 3530
SmlI CTYRAG 7 cut(s) 806, 1109, 1594, 2160, 2298, 2703, 3320
SmoI CTYRAG 7 cut(s) 806, 1109, 1594, 2160, 2298, 2703, 3320
SpeI ACTAGT 2 cut(s) 1388, 1405
SsiI CCGC 3 cut(s) 1802, 2795, 3623
SspI AATATT 2 cut(s) 180, 2485
SstI GAGCTC 1 cut(s) 1969
StyD4I CCNGG 4 cut(s) 1496, 2588, 2690, 2887
StyI CCWWGG 3 cut(s) 34, 156, 3193
TaaI ACNGT 8 cut(s) 220, 749, 1658, 1935, 2498, 2992, 3141, 3573
TatI WGTACW 2 cut(s) 1809, 2156
TauI GCSGC 1 cut(s) 1805
TscAI CASTG 5 cut(s) 653, 823, 858, 2503, 2631
TseFI GTSAC 8 cut(s) 662, 1724, 1785, 1935, 2401, 2492, 3141, 3342
TseI GCWGC 8 cut(s) 237, 251, 327, 830, 2468, 2631, 3056, 3116
Tsp45I GTSAC 8 cut(s) 662, 1724, 1785, 1935, 2401, 2492, 3141, 3342
TspRI CASTG 5 cut(s) 653, 823, 858, 2503, 2631
Vha464I CTTAAG 1 cut(s) 1594
VneI GTGCAC 1 cut(s) 750
VpaK11BI GGWCC 3 cut(s) 1427, 1957, 3256
VspI ATTAAT 1 cut(s) 1751
XagI CCTNNNNNAGG 1 cut(s) 1381
XapI RAATTY 7 cut(s) 151, 811, 895, 1091, 1584, 2314, 3155
XbaI TCTAGA 1 cut(s) 1487
XceI RCATGY 4 cut(s) 343, 681, 967, 1338
XcmI CCANNNNNNNNNTGG 1 cut(s) 1779
XhoI CTCGAG 1 cut(s) 2703
XmaJI CCTAGG 1 cut(s) 34
XmnI GAANNNNTTC 2 cut(s) 1407, 3188
Zsp2I ATGCAT 2 cut(s) 1017, 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.