RchiOBHm_Chr6g0300481

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000071
Physical Location & Seq
Reverse (-)
61308888 .. 61312490
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26985

Sequence Viewer

Length: 2091 bp
ATGAATTTCTCAATGATGCAATCAGAGAAACAGGCTATGAGGCATTCGGTAACTATCGAATTTTCTGTTATGGCCCAACGCAAACAGGGTCCTCCTCCAGGCTTCTCAGGACCTTCTCCACATCGTCCTCTAGGTAAACCAAATCCATATGCAAACAAAAGGTGCATTATCTGTGGGGAATTAGGTCATAGCAAGGAGCGGTGCTATGAAGTGATTGGTTACCCTGATTGGTGGGACTTCACCAAGAAACCGCGAAAGAATCTGGGCAAGGCCGCTGTTGCTACTAAAGAGGAAGTTTACCTAGACAATGCCTCCACTAATGTAGCGGCAGTAGCGCAGTCAGGTATGAAGGGTAAGGTTACTTTTAATAGTACATGGATAATTGATACAGGTGCATCTGACCATATGACCAATGACCCAAGTCTTGTGAAAAACCTTAGACTTTCCCCTCAAGACGTTGTCTCTACTGCTGATGGTACTCCAACTCCGGTCACCGGAGAAGGTTCTATTGCTTTATCTGATACCTTAACCCTTGAATCTGACATTCTGACTCGACGGATTCTTGGTTATGGTGTTAGAAGGGGGAAATTATACTATCTGGATCTGACAGAGACTGGAAAAAAACAGAAGCATCTTTTGGGACAAGCTAATCAGATCAACGGGGTAGAGAATGCGAAGGAAGCTGTATGGTTATGGCATCGCCGTTTAGGTCATCTATCCTTTCGTTATCTTAAGAAGCTGCAACCTCAATTGTTTTCAGTTGTTAGTGATTTGGATTTCCACTGTGACATCTGTGAACTGGCCAAGAGTCACCGTATTTCATATTCACCAAGTCTTAATAAAAGTCTTGTTCCTTTTATGAAGATTCACTCCGATGTCTGGGGTCCTGCGAAAATTTCTTCTCTTTCTGGAGCTCGGTATTTTGTAACATTTATTGATGATTGCACTCGCATGACATGGGTGTCATTACCGAAGAATAAGAGTGATGTATTTGGAATGTTTACCGAATTTCACAAAATGGTGGCAACTCAGTATCAACAATCCATCAGAGTGTTTCAGTCTGACAATGGTAGAGAGTTTGATGCATTGACGAATCCAAAATGGACCAAGGCGATGAATGAAGAATTGGAAGCTCTTCAGAAAAATGCAACATGGGAGCTAGTACCTATGCCGGTTGGAAAGAAGACTGTAGGAGGTCGTTGGGTATTTACTGTGAAGCTTAATGCAGATGGAACTATTAATAGATACAAAGCAAGGTTGGTTGCCAAAGGATATACACAACGCTATGGGATTGATTATAAGGAGACTTTTGCACCTGTGGCAAAGATTAATACTGTCCGGATTCTAATCTCACTTGCAGCAAACAAAGATTGGCCCTTGCACCAGTTTGATGTGAAGAATGCATTTCTTAATGGGAATTTGGAGGAAGAAGTGTACATGGATATGCCCCAGTCTCCAAGAGCTTGGTTTGGAAGATTTTCAAAGTCCATAAAAGCCTTTGGATATAAACAGAGCAATTCTGACCATACCTTGTTCATCAAGCGCAAGAATGCTAAGATTACAGCTCTTATTGTGTATGTTGATGACATGATTGTTACAGGAGATGATCCGAAGGAGATAAATGAATTGCAAAAGTATTTGTCAAAGGAGTCTGAAATGAAGGATCTAGGACAGCTAAAGTATTTTTTGGGTATTGAAGTTGCAAGGTCTAAGAAGGGGATTTCACTTTCACAGAGGAAATATGTTCTTGATTTACTTGCTGAAACGGGGATGCTGGACTGCCGACCCATCGAGACACCCATTGAGATGAATCACGGACTTGCTATCTATCCTGATCAAGTGCCAACTGATAAAGGGAGGTATCAACGTCTTGTAGGAAGAGCATATGGATGCAGTCTTTCGTATTTTGAGATTGGGCTGGTGATAAAACGGACAGACGTTCTACATCTGGGTACTTCACTTTTGTTGGAGGGAACCTTGTCACTTGGAGTAGCAAAAAGCAAAAAGTTGTTGCCAGATCAAGTGCAGAAGCTGAGTTCCGAGGTATGGCACATAGAGTATGAGAAATATTGTGGATTCGTAATGTCTTGA

Protein Analysis

696

Amino Acids

78.66

Weight (kDa)

9.28

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 126 - 173 7.6e-08 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 197 - 270 1.5e-15 GAG-pre-integrase domain
RVT_2 PF07727 382 - 483 6.7e-34 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 482 - 602 5.2e-24 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
AasI GACNNNNNNGTC 1 cut(s) 961
AccBSI CCGCTC 1 cut(s) 199
AccII CGCG 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 1338
AciI CCGC 4 cut(s) 199, 251, 273, 326
AclWI GGATC 3 cut(s) 609, 1601, 1671
AcoI YGGCCR 1 cut(s) 801
AcsI RAATTY 5 cut(s) 4, 59, 894, 1007, 1417
AcuI CTGAAG 1 cut(s) 1121
AfaI GTAC 5 cut(s) 373, 478, 1164, 1436, 1954
AfiI CCNNNNNNNGG 4 cut(s) 98, 494, 879, 2046
AflII CTTAAG 1 cut(s) 731
AgsI TTSAA 3 cut(s) 536, 1482, 1697
AjnI CCWGG 1 cut(s) 97
AjuI GAANNNNNNNTTGG 4 cut(s) 620, 652, 1109, 1141
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 5 cut(s) 466, 605, 1298, 1458, 1787
AlwI GGATC 3 cut(s) 609, 1601, 1671
AlwNI CAGNNNCTG 2 cut(s) 614, 2032
Aor13HI TCCGGA 1 cut(s) 1338
AoxI GGCC 4 cut(s) 72, 270, 801, 1373
ApeKI GCWGC 2 cut(s) 739, 1358
ApoI RAATTY 5 cut(s) 4, 59, 894, 1007, 1417
AseI ATTAAT 2 cut(s) 1239, 1329
Asp700I GAANNNNTTC 2 cut(s) 1134, 1477
AspLEI GCGC 2 cut(s) 337, 1545
AspS9I GGNCC 6 cut(s) 73, 89, 110, 884, 1104, 1374
AsuHPI GGTGA 5 cut(s) 232, 484, 803, 819, 1933
AvaII GGWCC 4 cut(s) 89, 110, 884, 1104
BalI TGGCCA 1 cut(s) 803
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 1190
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 2 cut(s) 726, 1370
BccI CCATC 4 cut(s) 467, 1052, 1223, 1796
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 1 cut(s) 99
BclI TGATCA 1 cut(s) 1834
BcoDI GTCTC 5 cut(s) 466, 605, 1298, 1458, 1787
BfaI CTAG 4 cut(s) 131, 302, 1160, 1667
BfmI CTRYAG 1 cut(s) 1188
BfrI CTTAAG 1 cut(s) 731
BisI GCNGC 4 cut(s) 273, 327, 740, 1359
BlsI GCNGC 4 cut(s) 274, 328, 741, 1360
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 4 cut(s) 89, 110, 884, 1104
BmgT120I GGNCC 6 cut(s) 73, 89, 110, 884, 1104, 1374
BmiI GGNNCC 3 cut(s) 90, 885, 1975
BmrFI CCNGG 1 cut(s) 99
BmrI ACTGGG 1 cut(s) 1444
BmsI GCATC 7 cut(s) 6, 404, 640, 706, 1072, 1761, 1880
BmuI ACTGGG 1 cut(s) 1444
BoxI GACNNNNGTC 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 1190
BpmI CTGGAG 2 cut(s) 81, 930
BpuEI CTTGAG 1 cut(s) 435
BsaBI GATNNNNATC 1 cut(s) 1346
BsaJI CCNNGG 2 cut(s) 1107, 2040
BsaWI WCCGGW 3 cut(s) 487, 494, 1338
BsaXI ACNNNNNCTCC 4 cut(s) 296, 326, 469, 499
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 494, 879, 2046
Bse118I RCCGGY 1 cut(s) 1171
Bse1I ACTGG 4 cut(s) 619, 804, 1384, 1450
Bse8I GATNNNNATC 1 cut(s) 1346
BseAI TCCGGA 1 cut(s) 1338
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 1107, 2040
BseGI GGATG 2 cut(s) 1776, 1895
BseJI GATNNNNATC 1 cut(s) 1346
BseLI CCNNNNNNNGG 4 cut(s) 98, 494, 879, 2046
BseMII CTCAG 3 cut(s) 120, 1043, 2024
BseNI ACTGG 4 cut(s) 619, 804, 1384, 1450
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 2 cut(s) 726, 1370
BsgI GTGCAG 1 cut(s) 2045
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 4 cut(s) 74, 272, 803, 1375
BsiHKAI GWGCWC 1 cut(s) 916
BsiSI CCGG 4 cut(s) 488, 495, 1172, 1339
BslFI GGGAC 2 cut(s) 248, 654
BslI CCNNNNNNNGG 4 cut(s) 98, 494, 879, 2046
BsmAI GTCTC 5 cut(s) 466, 605, 1298, 1458, 1787
BsmFI GGGAC 2 cut(s) 248, 654
BsmI GAATGC 4 cut(s) 43, 676, 1405, 1555
BsnI GGCC 4 cut(s) 74, 272, 803, 1375
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 1338
Bsp1407I TGTACA 1 cut(s) 1434
Bsp143I GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
BspACI CCGC 4 cut(s) 199, 251, 273, 326
BspANI GGCC 4 cut(s) 74, 272, 803, 1375
BspCNI CTCAG 3 cut(s) 119, 1042, 2025
BspEI TCCGGA 1 cut(s) 1338
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 90, 885, 1975
BspPI GGATC 3 cut(s) 609, 1601, 1671
BspQI GCTCTTC 2 cut(s) 1140, 1873
BspTI CTTAAG 1 cut(s) 731
BsrBI CCGCTC 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 1171
BsrGI TGTACA 1 cut(s) 1434
BsrI ACTGG 4 cut(s) 619, 804, 1384, 1450
BssAI RCCGGY 1 cut(s) 1171
BssECI CCNNGG 2 cut(s) 1107, 2040
BssMI GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
BssT1I CCWWGG 1 cut(s) 1107
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 5 cut(s) 785, 815, 1189, 1213, 1336
Bst6I CTCTTC 2 cut(s) 1140, 1873
BstAFI CTTAAG 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 1434
BstDEI CTNAG 6 cut(s) 106, 437, 1029, 1554, 1710, 2033
BstEII GGTNACC 2 cut(s) 218, 490
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 1776, 1895
BstFNI CGCG 1 cut(s) 253
BstHHI GCGC 2 cut(s) 337, 1545
BstKTI GATC 6 cut(s) 604, 657, 1609, 1666, 1837, 2020
BstMAI GTCTC 5 cut(s) 466, 605, 1298, 1458, 1787
BstMBI GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
BstMWI GCNNNNNNNGC 4 cut(s) 278, 332, 680, 1319
BstNI CCWGG 1 cut(s) 99
BstPAI GACNNNNGTC 1 cut(s) 420
BstPI GGTNACC 2 cut(s) 218, 490
BstSCI CCNGG 1 cut(s) 97
BstSFI CTRYAG 1 cut(s) 1188
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 2 cut(s) 726, 1370
BstV2I GAAGAC 1 cut(s) 1190
BstX2I RGATCY 2 cut(s) 601, 1663
BstXI CCANNNNNNTGG 1 cut(s) 1464
BstYI RGATCY 2 cut(s) 601, 1663
BsuRI GGCC 4 cut(s) 74, 272, 803, 1375
BtgZI GCGATG 2 cut(s) 683, 1127
BtsCI GGATG 2 cut(s) 1776, 1895
BtsIMutI CAGTG 1 cut(s) 781
CaiI CAGNNNCTG 2 cut(s) 614, 2032
CfoI GCGC 2 cut(s) 337, 1545
Cfr10I RCCGGY 1 cut(s) 1171
Cfr13I GGNCC 6 cut(s) 73, 89, 110, 884, 1104, 1374
Csp6I GTAC 5 cut(s) 372, 477, 1163, 1435, 1953
CviAII CATG 6 cut(s) 375, 952, 957, 1152, 1438, 1588
CviQI GTAC 5 cut(s) 372, 477, 1163, 1435, 1953
DdeI CTNAG 6 cut(s) 106, 437, 1029, 1554, 1710, 2033
DpnI GATC 6 cut(s) 603, 656, 1608, 1665, 1836, 2019
DpnII GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
DrdI GACNNNNNNGTC 1 cut(s) 961
DseDI GACNNNNNNGTC 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 801
Eam1104I CTCTTC 2 cut(s) 1140, 1873
EarI CTCTTC 2 cut(s) 1140, 1873
Ecl136II GAGCTC 1 cut(s) 914
Eco130I CCWWGG 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 916
Eco47I GGWCC 4 cut(s) 89, 110, 884, 1104
Eco53kI GAGCTC 1 cut(s) 914
Eco57I CTGAAG 1 cut(s) 1121
Eco91I GGTNACC 2 cut(s) 218, 490
EcoICRI GAGCTC 1 cut(s) 914
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoO109I RGGNCCY 3 cut(s) 89, 110, 884
EcoO65I GGTNACC 2 cut(s) 218, 490
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 1107
EcoT22I ATGCAT 2 cut(s) 1087, 1405
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 1107
FaeI CATG 6 cut(s) 378, 955, 960, 1155, 1441, 1591
FaqI GGGAC 2 cut(s) 248, 654
FatI CATG 6 cut(s) 374, 951, 956, 1151, 1437, 1587
FauNDI CATATG 3 cut(s) 148, 405, 1885
FbaI TGATCA 1 cut(s) 1834
Fnu4HI GCNGC 4 cut(s) 273, 327, 740, 1359
FokI GGATG 2 cut(s) 1783, 1902
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 4 cut(s) 273, 327, 740, 1359
FspBI CTAG 4 cut(s) 131, 302, 1160, 1667
GlaI GCGC 2 cut(s) 336, 1544
GluI GCNGC 4 cut(s) 273, 327, 740, 1359
GsuI CTGGAG 2 cut(s) 81, 930
HaeIII GGCC 4 cut(s) 74, 272, 803, 1375
HapII CCGG 4 cut(s) 488, 495, 1172, 1339
HhaI GCGC 2 cut(s) 337, 1545
Hin1II CATG 6 cut(s) 378, 955, 960, 1155, 1441, 1591
Hin6I GCGC 2 cut(s) 335, 1543
HinP1I GCGC 2 cut(s) 335, 1543
HindIII AAGCTT 1 cut(s) 1217
HpaII CCGG 4 cut(s) 488, 495, 1172, 1339
HphI GGTGA 5 cut(s) 232, 484, 803, 819, 1933
Hpy166II GTNNAC 5 cut(s) 137, 298, 797, 1002, 1435
Hpy188III TCNNGA 9 cut(s) 108, 452, 599, 909, 1339, 1748, 1792, 1832, 2088
Hpy8I GTNNAC 5 cut(s) 137, 298, 797, 1002, 1435
Hpy99I CGWCG 1 cut(s) 558
HpyAV CCTTC 8 cut(s) 123, 343, 494, 573, 670, 1606, 1654, 1708
HpyCH4III ACNGT 5 cut(s) 785, 815, 1189, 1213, 1336
HpyCH4IV ACGT 3 cut(s) 456, 1867, 1938
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 332, 680, 1319
HpyF3I CTNAG 6 cut(s) 106, 437, 1029, 1554, 1710, 2033
HpySE526I ACGT 3 cut(s) 456, 1867, 1938
Hsp92II CATG 6 cut(s) 378, 955, 960, 1155, 1441, 1591
HspAI GCGC 2 cut(s) 335, 1543
Kpn2I TCCGGA 1 cut(s) 1338
Ksp22I TGATCA 1 cut(s) 1834
Kzo9I GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
LguI GCTCTTC 2 cut(s) 1140, 1873
LmnI GCTCC 3 cut(s) 196, 911, 1156
Lsp1109I GCAGC 2 cut(s) 726, 1370
LweI GCATC 7 cut(s) 6, 404, 640, 706, 1072, 1761, 1880
MaeI CTAG 4 cut(s) 131, 302, 1160, 1667
MaeII ACGT 3 cut(s) 456, 1867, 1938
MaeIII GTNAC 9 cut(s) 49, 218, 358, 490, 785, 809, 925, 1594, 1980
MalI GATC 6 cut(s) 603, 656, 1608, 1665, 1836, 2019
MbiI CCGCTC 1 cut(s) 199
MboI GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
MfeI CAATTG 1 cut(s) 749
MflI RGATCY 2 cut(s) 601, 1663
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 803
MluNI TGGCCA 1 cut(s) 803
MlyI GAGTC 3 cut(s) 544, 817, 1658
MmeI TCCRAC 3 cut(s) 506, 1156, 1947
Mox20I TGGCCA 1 cut(s) 803
Mph1103I ATGCAT 2 cut(s) 1087, 1405
MroI TCCGGA 1 cut(s) 1338
MroXI GAANNNNTTC 2 cut(s) 1134, 1477
MscI TGGCCA 1 cut(s) 803
MseI TTAA 8 cut(s) 366, 527, 732, 837, 1221, 1239, 1329, 1410
MslI CAYNNNNRTG 6 cut(s) 873, 950, 1049, 1442, 1805, 1888
Msp20I TGGCCA 1 cut(s) 803
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 4 cut(s) 488, 495, 1172, 1339
MspR9I CCNGG 1 cut(s) 99
MunI CAATTG 1 cut(s) 749
Mva1269I GAATGC 4 cut(s) 43, 676, 1405, 1555
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 4 cut(s) 278, 332, 680, 1319
NdeI CATATG 3 cut(s) 148, 405, 1885
NdeII GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
NlaIII CATG 6 cut(s) 378, 955, 960, 1155, 1441, 1591
NlaIV GGNNCC 3 cut(s) 90, 885, 1975
NmuCI GTSAC 4 cut(s) 490, 785, 809, 1980
NsiI ATGCAT 2 cut(s) 1087, 1405
PciSI GCTCTTC 2 cut(s) 1140, 1873
PctI GAATGC 4 cut(s) 43, 676, 1405, 1555
PdmI GAANNNNTTC 2 cut(s) 1134, 1477
PfeI GAWTC 8 cut(s) 259, 536, 559, 865, 1093, 1342, 1810, 2076
PflFI GACNNNGTC 1 cut(s) 458
PkrI GCNGC 4 cut(s) 274, 328, 741, 1360
PleI GAGTC 3 cut(s) 544, 816, 1657
PpsI GAGTC 3 cut(s) 544, 816, 1657
PpuMI RGGWCCY 3 cut(s) 89, 110, 884
PshAI GACNNNNGTC 1 cut(s) 420
PshBI ATTAAT 2 cut(s) 1239, 1329
PsiI TTATAA 1 cut(s) 1299
Psp124BI GAGCTC 1 cut(s) 916
Psp5II RGGWCCY 3 cut(s) 89, 110, 884
Psp6I CCWGG 1 cut(s) 97
PspEI GGTNACC 2 cut(s) 218, 490
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 3 cut(s) 90, 885, 1975
PspPI GGNCC 6 cut(s) 73, 89, 110, 884, 1104, 1374
PspPPI RGGWCCY 3 cut(s) 89, 110, 884
PstNI CAGNNNCTG 2 cut(s) 614, 2032
PsuI RGATCY 2 cut(s) 601, 1663
PsyI GACNNNGTC 1 cut(s) 458
RsaI GTAC 5 cut(s) 373, 478, 1164, 1436, 1954
RsaNI GTAC 5 cut(s) 372, 477, 1163, 1435, 1953
RseI CAYNNNNRTG 6 cut(s) 873, 950, 1049, 1442, 1805, 1888
SacI GAGCTC 1 cut(s) 916
SapI GCTCTTC 2 cut(s) 1140, 1873
SaqAI TTAA 8 cut(s) 366, 527, 732, 837, 1221, 1239, 1329, 1410
SatI GCNGC 4 cut(s) 273, 327, 740, 1359
Sau3AI GATC 6 cut(s) 601, 654, 1606, 1663, 1834, 2017
Sau96I GGNCC 6 cut(s) 73, 89, 110, 884, 1104, 1374
SchI GAGTC 3 cut(s) 544, 817, 1658
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 7 cut(s) 6, 404, 640, 706, 1072, 1761, 1880
SfcI CTRYAG 1 cut(s) 1188
SinI GGWCC 4 cut(s) 89, 110, 884, 1104
SmiMI CAYNNNNRTG 6 cut(s) 873, 950, 1049, 1442, 1805, 1888
SmlI CTYRAG 2 cut(s) 450, 731
SmoI CTYRAG 2 cut(s) 450, 731
SsiI CCGC 4 cut(s) 199, 251, 273, 326
SspI AATATT 1 cut(s) 2069
SspMI CTAG 4 cut(s) 131, 302, 1160, 1667
SstI GAGCTC 1 cut(s) 916
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 5 cut(s) 785, 815, 1189, 1213, 1336
TaiI ACGT 3 cut(s) 459, 1870, 1941
TaqI TCGA 3 cut(s) 57, 553, 1791
TatI WGTACW 2 cut(s) 371, 1434
TauI GCSGC 2 cut(s) 275, 329
TfiI GAWTC 8 cut(s) 259, 536, 559, 865, 1093, 1342, 1810, 2076
Tru1I TTAA 8 cut(s) 366, 527, 732, 837, 1221, 1239, 1329, 1410
Tru9I TTAA 8 cut(s) 366, 527, 732, 837, 1221, 1239, 1329, 1410
TscAI CASTG 1 cut(s) 788
TseFI GTSAC 4 cut(s) 490, 785, 809, 1980
TseI GCWGC 2 cut(s) 739, 1358
Tsp45I GTSAC 4 cut(s) 490, 785, 809, 1980
TspGWI ACGGA 3 cut(s) 571, 1830, 1945
TspRI CASTG 1 cut(s) 788
Tth111I GACNNNGTC 1 cut(s) 458
Vha464I CTTAAG 1 cut(s) 731
VpaK11BI GGWCC 4 cut(s) 89, 110, 884, 1104
VspI ATTAAT 2 cut(s) 1239, 1329
XagI CCTNNNNNAGG 1 cut(s) 96
XapI RAATTY 5 cut(s) 4, 59, 894, 1007, 1417
XmnI GAANNNNTTC 2 cut(s) 1134, 1477
XspI CTAG 4 cut(s) 131, 302, 1160, 1667
Zsp2I ATGCAT 2 cut(s) 1087, 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.