Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 546, 908 |
| Acc36I | ACCTGC | 1 cut(s) | 1567 |
| Acc65I | GGTACC | 1 cut(s) | 1108 |
| AccB1I | GGYRCC | 3 cut(s) | 479, 1108, 1398 |
| AccI | GTMKAC | 2 cut(s) | 709, 733 |
| AccII | CGCG | 2 cut(s) | 531, 1782 |
| AciI | CCGC | 4 cut(s) | 10, 169, 394, 1622 |
| AclWI | GGATC | 2 cut(s) | 126, 1157 |
| AcoI | YGGCCR | 1 cut(s) | 661 |
| AcsI | RAATTY | 2 cut(s) | 865, 1704 |
| AcuI | CTGAAG | 1 cut(s) | 1332 |
| AcyI | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| AfaI | GTAC | 8 cut(s) | 612, 995, 1055, 1110, 1275, 1430, 1581, 1652 |
| AfeI | AGCGCT | 1 cut(s) | 491 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 15, 37, 673, 698, 1331, 1522 |
| AflII | CTTAAG | 1 cut(s) | 1313 |
| AflIII | ACRYGT | 1 cut(s) | 613 |
| AgsI | TTSAA | 5 cut(s) | 218, 457, 1491, 1567, 1709 |
| AjiI | CACGTC | 1 cut(s) | 353 |
| AjnI | CCWGG | 3 cut(s) | 36, 697, 1324 |
| AluBI | AGCT | 5 cut(s) | 235, 500, 599, 960, 1195 |
| AluI | AGCT | 5 cut(s) | 235, 500, 599, 960, 1195 |
| Alw26I | GTCTC | 2 cut(s) | 29, 346 |
| AlwI | GGATC | 2 cut(s) | 126, 1157 |
| Aor51HI | AGCGCT | 1 cut(s) | 491 |
| AoxI | GGCC | 5 cut(s) | 620, 661, 1077, 1506, 1623 |
| ApeKI | GCWGC | 7 cut(s) | 232, 605, 688, 960, 1370, 1526, 1637 |
| ApoI | RAATTY | 2 cut(s) | 865, 1704 |
| Asp718I | GGTACC | 1 cut(s) | 1108 |
| AspLEI | GCGC | 4 cut(s) | 492, 562, 1782, 1784 |
| AspS9I | GGNCC | 2 cut(s) | 402, 983 |
| AsuHPI | GGTGA | 5 cut(s) | 131, 153, 193, 194, 1727 |
| AvaII | GGWCC | 2 cut(s) | 402, 983 |
| AxyI | CCTNAGG | 2 cut(s) | 1269, 1590 |
| BaeGI | GKGCMC | 2 cut(s) | 273, 1403 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1092, 1125 |
| BalI | TGGCCA | 1 cut(s) | 663 |
| BanI | GGYRCC | 3 cut(s) | 479, 1108, 1398 |
| BanII | GRGCYC | 2 cut(s) | 8, 382 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 267, 299, 1184, 1216 |
| BbsI | GAAGAC | 1 cut(s) | 1186 |
| BbvCI | CCTCAGC | 1 cut(s) | 1503 |
| BbvI | GCAGC | 7 cut(s) | 244, 592, 675, 947, 1382, 1538, 1649 |
| BccI | CCATC | 6 cut(s) | 54, 78, 144, 213, 809, 1022 |
| BceAI | ACGGC | 2 cut(s) | 493, 1220 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 994, 1028 |
| BciT130I | CCWGG | 3 cut(s) | 38, 699, 1326 |
| BciVI | GTATCC | 1 cut(s) | 421 |
| BcoDI | GTCTC | 2 cut(s) | 29, 346 |
| BfaI | CTAG | 1 cut(s) | 1215 |
| BfmI | CTRYAG | 3 cut(s) | 230, 373, 606 |
| BfoI | RGCGCY | 1 cut(s) | 493 |
| BfrI | CTTAAG | 1 cut(s) | 1313 |
| BfuAI | ACCTGC | 1 cut(s) | 1567 |
| BfuI | GTATCC | 1 cut(s) | 421 |
| BglII | AGATCT | 1 cut(s) | 976 |
| BisI | GCNGC | 9 cut(s) | 169, 233, 606, 689, 961, 1371, 1527, 1623, 1638 |
| BlsI | GCNGC | 9 cut(s) | 170, 234, 607, 690, 962, 1372, 1528, 1624, 1639 |
| BmcAI | AGTACT | 1 cut(s) | 995 |
| Bme1390I | CCNGG | 3 cut(s) | 38, 699, 1326 |
| Bme18I | GGWCC | 2 cut(s) | 402, 983 |
| BmgBI | CACGTC | 1 cut(s) | 353 |
| BmgT120I | GGNCC | 2 cut(s) | 402, 983 |
| BmiI | GGNNCC | 7 cut(s) | 7, 481, 985, 1110, 1119, 1266, 1400 |
| BmrFI | CCNGG | 3 cut(s) | 38, 699, 1326 |
| BmrI | ACTGGG | 2 cut(s) | 1165, 1399 |
| BmsI | GCATC | 3 cut(s) | 637, 776, 1372 |
| BmuI | ACTGGG | 2 cut(s) | 1165, 1399 |
| BoxI | GACNNNNGTC | 1 cut(s) | 338 |
| BpiI | GAAGAC | 1 cut(s) | 1186 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1503, 1805 |
| BpuEI | CTTGAG | 3 cut(s) | 404, 926, 974 |
| BsaAI | YACGTR | 2 cut(s) | 614, 1230 |
| BsaHI | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| BsaI | GGTCTC | 1 cut(s) | 346 |
| BsaJI | CCNNGG | 5 cut(s) | 405, 513, 1023, 1104, 1324 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 68, 98 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 15, 37, 673, 698, 1331, 1522 |
| Bse118I | RCCGGY | 1 cut(s) | 178 |
| Bse1I | ACTGG | 4 cut(s) | 1171, 1394, 1646, 1747 |
| Bse21I | CCTNAGG | 2 cut(s) | 1269, 1590 |
| Bse3DI | GCAATG | 1 cut(s) | 1632 |
| BseBI | CCWGG | 3 cut(s) | 38, 699, 1326 |
| BseDI | CCNNGG | 5 cut(s) | 405, 513, 1023, 1104, 1324 |
| BseGI | GGATG | 4 cut(s) | 643, 801, 1033, 1585 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 15, 37, 673, 698, 1331, 1522 |
| BseMI | GCAATG | 1 cut(s) | 1632 |
| BseMII | CTCAG | 6 cut(s) | 344, 663, 1128, 1494, 1558, 1581 |
| BseNI | ACTGG | 4 cut(s) | 1171, 1394, 1646, 1747 |
| BsePI | GCGCGC | 1 cut(s) | 1780 |
| BseRI | GAGGAG | 4 cut(s) | 336, 684, 689, 1088 |
| BseSI | GKGCMC | 2 cut(s) | 273, 1403 |
| BseXI | GCAGC | 7 cut(s) | 244, 592, 675, 947, 1382, 1538, 1649 |
| BsgI | GTGCAG | 1 cut(s) | 47 |
| Bsh1236I | CGCG | 2 cut(s) | 531, 1782 |
| BshFI | GGCC | 5 cut(s) | 622, 663, 1079, 1508, 1625 |
| BshNI | GGYRCC | 3 cut(s) | 479, 1108, 1398 |
| BsiSI | CCGG | 2 cut(s) | 179, 1080 |
| BslFI | GGGAC | 2 cut(s) | 730, 1307 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 15, 37, 673, 698, 1331, 1522 |
| BsmAI | GTCTC | 2 cut(s) | 29, 346 |
| BsmBI | CGTCTC | 1 cut(s) | 29 |
| BsmFI | GGGAC | 2 cut(s) | 730, 1307 |
| BsnI | GGCC | 5 cut(s) | 622, 663, 1079, 1508, 1625 |
| Bso31I | GGTCTC | 1 cut(s) | 346 |
| Bsp1286I | GDGCHC | 4 cut(s) | 8, 273, 382, 1403 |
| Bsp143I | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| Bsp19I | CCATGG | 1 cut(s) | 405 |
| BspACI | CCGC | 4 cut(s) | 10, 169, 394, 1622 |
| BspANI | GGCC | 5 cut(s) | 622, 663, 1079, 1508, 1625 |
| BspCNI | CTCAG | 6 cut(s) | 343, 664, 1129, 1495, 1557, 1582 |
| BspFNI | CGCG | 2 cut(s) | 531, 1782 |
| BspLI | GGNNCC | 7 cut(s) | 7, 481, 985, 1110, 1119, 1266, 1400 |
| BspMAI | CTGCAG | 2 cut(s) | 234, 610 |
| BspMI | ACCTGC | 1 cut(s) | 1567 |
| BspPI | GGATC | 2 cut(s) | 126, 1157 |
| BspQI | GCTCTTC | 1 cut(s) | 1460 |
| BspT107I | GGYRCC | 3 cut(s) | 479, 1108, 1398 |
| BspTI | CTTAAG | 1 cut(s) | 1313 |
| BspTNI | GGTCTC | 1 cut(s) | 346 |
| BsrDI | GCAATG | 1 cut(s) | 1632 |
| BsrFI | RCCGGY | 1 cut(s) | 178 |
| BsrI | ACTGG | 4 cut(s) | 1171, 1394, 1646, 1747 |
| BssAI | RCCGGY | 1 cut(s) | 178 |
| BssECI | CCNNGG | 5 cut(s) | 405, 513, 1023, 1104, 1324 |
| BssHII | GCGCGC | 1 cut(s) | 1780 |
| BssMI | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| BssNI | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| BssT1I | CCWWGG | 4 cut(s) | 405, 513, 1023, 1104 |
| Bst2UI | CCWGG | 3 cut(s) | 38, 699, 1326 |
| Bst4CI | ACNGT | 8 cut(s) | 268, 583, 877, 1433, 1486, 1517, 1685, 1693 |
| Bst6I | CTCTTC | 1 cut(s) | 1460 |
| BstACI | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| BstAFI | CTTAAG | 1 cut(s) | 1313 |
| BstBAI | YACGTR | 2 cut(s) | 614, 1230 |
| BstC8I | GCNNGC | 2 cut(s) | 1018, 1782 |
| BstDEI | CTNAG | 9 cut(s) | 330, 672, 1137, 1269, 1503, 1544, 1553, 1590, 1805 |
| BstDSI | CCRYGG | 1 cut(s) | 405 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 35, 671, 1329 |
| BstF5I | GGATG | 4 cut(s) | 643, 801, 1033, 1585 |
| BstFNI | CGCG | 2 cut(s) | 531, 1782 |
| BstH2I | RGCGCY | 1 cut(s) | 493 |
| BstHHI | GCGC | 4 cut(s) | 492, 562, 1782, 1784 |
| BstKTI | GATC | 7 cut(s) | 134, 262, 940, 979, 1165, 1418, 1496 |
| BstMAI | GTCTC | 2 cut(s) | 29, 346 |
| BstMBI | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 566, 605, 1391, 1666, 1796 |
| BstNI | CCWGG | 3 cut(s) | 38, 699, 1326 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 338 |
| BstSCI | CCNGG | 3 cut(s) | 36, 697, 1324 |
| BstSFI | CTRYAG | 3 cut(s) | 230, 373, 606 |
| BstSLI | GKGCMC | 2 cut(s) | 273, 1403 |
| BstSNI | TACGTA | 1 cut(s) | 1230 |
| BstUI | CGCG | 2 cut(s) | 531, 1782 |
| BstV1I | GCAGC | 7 cut(s) | 244, 592, 675, 947, 1382, 1538, 1649 |
| BstV2I | GAAGAC | 1 cut(s) | 1186 |
| BstX2I | RGATCY | 2 cut(s) | 976, 1162 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 812 |
| BstYI | RGATCY | 2 cut(s) | 976, 1162 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1269, 1590 |
| BsuI | GTATCC | 1 cut(s) | 421 |
| BsuRI | GGCC | 5 cut(s) | 622, 663, 1079, 1508, 1625 |
| BtgI | CCRYGG | 1 cut(s) | 405 |
| BtgZI | GCGATG | 1 cut(s) | 1395 |
| BtrI | CACGTC | 1 cut(s) | 353 |
| BtsCI | GGATG | 4 cut(s) | 643, 801, 1033, 1585 |
| BtsI | GCAGTG | 1 cut(s) | 59 |
| BtsIMutI | CAGTG | 2 cut(s) | 59, 1653 |
| BveI | ACCTGC | 1 cut(s) | 1567 |
| Cac8I | GCNNGC | 2 cut(s) | 1018, 1782 |
| CfoI | GCGC | 4 cut(s) | 492, 562, 1782, 1784 |
| Cfr10I | RCCGGY | 1 cut(s) | 178 |
| Cfr13I | GGNCC | 2 cut(s) | 402, 983 |
| CseI | GACGC | 3 cut(s) | 8, 199, 1160 |
| Csp6I | GTAC | 8 cut(s) | 611, 994, 1054, 1109, 1274, 1429, 1580, 1651 |
| CviQI | GTAC | 8 cut(s) | 611, 994, 1054, 1109, 1274, 1429, 1580, 1651 |
| DdeI | CTNAG | 9 cut(s) | 330, 672, 1137, 1269, 1503, 1544, 1553, 1590, 1805 |
| DpnI | GATC | 7 cut(s) | 133, 261, 939, 978, 1164, 1417, 1495 |
| DpnII | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| EaeI | YGGCCR | 1 cut(s) | 661 |
| Eam1104I | CTCTTC | 1 cut(s) | 1460 |
| EarI | CTCTTC | 1 cut(s) | 1460 |
| Eco105I | TACGTA | 1 cut(s) | 1230 |
| Eco130I | CCWWGG | 4 cut(s) | 405, 513, 1023, 1104 |
| Eco24I | GRGCYC | 2 cut(s) | 8, 382 |
| Eco31I | GGTCTC | 1 cut(s) | 346 |
| Eco47I | GGWCC | 2 cut(s) | 402, 983 |
| Eco47III | AGCGCT | 1 cut(s) | 491 |
| Eco57I | CTGAAG | 1 cut(s) | 1332 |
| Eco81I | CCTNAGG | 2 cut(s) | 1269, 1590 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 35, 671, 1329 |
| EcoRI | GAATTC | 2 cut(s) | 865, 1704 |
| EcoRII | CCWGG | 3 cut(s) | 36, 697, 1324 |
| EcoT14I | CCWWGG | 4 cut(s) | 405, 513, 1023, 1104 |
| EcoT38I | GRGCYC | 2 cut(s) | 8, 382 |
| ErhI | CCWWGG | 4 cut(s) | 405, 513, 1023, 1104 |
| Esp3I | CGTCTC | 1 cut(s) | 29 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 489, 521 |
| FaqI | GGGAC | 2 cut(s) | 730, 1307 |
| FauI | CCCGC | 1 cut(s) | 17 |
| FblI | GTMKAC | 2 cut(s) | 709, 733 |
| Fnu4HI | GCNGC | 9 cut(s) | 169, 233, 606, 689, 961, 1371, 1527, 1623, 1638 |
| FokI | GGATG | 4 cut(s) | 650, 788, 1040, 1572 |
| FriOI | GRGCYC | 2 cut(s) | 8, 382 |
| Fsp4HI | GCNGC | 9 cut(s) | 169, 233, 606, 689, 961, 1371, 1527, 1623, 1638 |
| FspBI | CTAG | 1 cut(s) | 1215 |
| GlaI | GCGC | 4 cut(s) | 491, 561, 1781, 1783 |
| GluI | GCNGC | 9 cut(s) | 169, 233, 606, 689, 961, 1371, 1527, 1623, 1638 |
| HaeII | RGCGCY | 1 cut(s) | 493 |
| HaeIII | GGCC | 5 cut(s) | 622, 663, 1079, 1508, 1625 |
| HapII | CCGG | 2 cut(s) | 179, 1080 |
| HgaI | GACGC | 3 cut(s) | 8, 199, 1160 |
| HhaI | GCGC | 4 cut(s) | 492, 562, 1782, 1784 |
| Hin1I | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| Hin6I | GCGC | 4 cut(s) | 490, 560, 1780, 1782 |
| HinP1I | GCGC | 4 cut(s) | 490, 560, 1780, 1782 |
| HincII | GTYRAC | 4 cut(s) | 710, 734, 1046, 1134 |
| HindII | GTYRAC | 4 cut(s) | 710, 734, 1046, 1134 |
| HinfI | GANTC | 1 cut(s) | 519 |
| HpaI | GTTAAC | 1 cut(s) | 1134 |
| HpaII | CCGG | 2 cut(s) | 179, 1080 |
| HphI | GGTGA | 5 cut(s) | 131, 153, 193, 194, 1727 |
| Hpy188I | TCNGA | 4 cut(s) | 53, 264, 333, 1351 |
| Hpy188III | TCNNGA | 4 cut(s) | 943, 1491, 1589, 1697 |
| Hpy99I | CGWCG | 2 cut(s) | 24, 193 |
| HpyAV | CCTTC | 9 cut(s) | 89, 293, 429, 527, 661, 919, 1176, 1356, 1801 |
| HpyCH4III | ACNGT | 8 cut(s) | 268, 583, 877, 1433, 1486, 1517, 1685, 1693 |
| HpyCH4IV | ACGT | 4 cut(s) | 352, 613, 1048, 1229 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 566, 605, 1391, 1666, 1796 |
| HpyF3I | CTNAG | 9 cut(s) | 330, 672, 1137, 1269, 1503, 1544, 1553, 1590, 1805 |
| HpySE526I | ACGT | 4 cut(s) | 352, 613, 1048, 1229 |
| Hsp92I | GRCGYC | 3 cut(s) | 19, 191, 1152 |
| HspAI | GCGC | 4 cut(s) | 490, 560, 1780, 1782 |
| KpnI | GGTACC | 1 cut(s) | 1112 |
| KspAI | GTTAAC | 1 cut(s) | 1134 |
| Kzo9I | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| LguI | GCTCTTC | 1 cut(s) | 1460 |
| LmnI | GCTCC | 5 cut(s) | 11, 240, 1025, 1117, 1264 |
| Lsp1109I | GCAGC | 7 cut(s) | 244, 592, 675, 947, 1382, 1538, 1649 |
| LweI | GCATC | 3 cut(s) | 637, 776, 1372 |
| MaeI | CTAG | 1 cut(s) | 1215 |
| MaeII | ACGT | 4 cut(s) | 352, 613, 1048, 1229 |
| MaeIII | GTNAC | 7 cut(s) | 274, 577, 1148, 1420, 1449, 1480, 1610 |
| MalI | GATC | 7 cut(s) | 133, 261, 939, 978, 1164, 1417, 1495 |
| MboI | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| MboII | GAAGA | 3 cut(s) | 1191, 1425, 1477 |
| MfeI | CAATTG | 1 cut(s) | 930 |
| MflI | RGATCY | 2 cut(s) | 976, 1162 |
| MhlI | GDGCHC | 4 cut(s) | 8, 273, 382, 1403 |
| MlsI | TGGCCA | 1 cut(s) | 663 |
| MluCI | AATT | 8 cut(s) | 572, 810, 865, 930, 1032, 1038, 1253, 1704 |
| MluNI | TGGCCA | 1 cut(s) | 663 |
| MmeI | TCCRAC | 1 cut(s) | 176 |
| Mox20I | TGGCCA | 1 cut(s) | 663 |
| MscI | TGGCCA | 1 cut(s) | 663 |
| MseI | TTAA | 8 cut(s) | 759, 954, 966, 972, 1133, 1284, 1314, 1722 |
| MslI | CAYNNNNRTG | 1 cut(s) | 179 |
| Msp20I | TGGCCA | 1 cut(s) | 663 |
| MspA1I | CMGCKG | 2 cut(s) | 12, 960 |
| MspCI | CTTAAG | 1 cut(s) | 1313 |
| MspI | CCGG | 2 cut(s) | 179, 1080 |
| MspR9I | CCNGG | 3 cut(s) | 38, 699, 1326 |
| MunI | CAATTG | 1 cut(s) | 930 |
| MvaI | CCWGG | 3 cut(s) | 38, 699, 1326 |
| MvnI | CGCG | 2 cut(s) | 531, 1782 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 566, 605, 1391, 1666, 1796 |
| NcoI | CCATGG | 1 cut(s) | 405 |
| NdeII | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| NlaIV | GGNNCC | 7 cut(s) | 7, 481, 985, 1110, 1119, 1266, 1400 |
| NmuCI | GTSAC | 4 cut(s) | 1148, 1449, 1480, 1610 |
| PauI | GCGCGC | 1 cut(s) | 1780 |
| PciSI | GCTCTTC | 1 cut(s) | 1460 |
| PfeI | GAWTC | 1 cut(s) | 519 |
| PkrI | GCNGC | 9 cut(s) | 170, 234, 607, 690, 962, 1372, 1528, 1624, 1639 |
| Ppu21I | YACGTR | 2 cut(s) | 614, 1230 |
| PshAI | GACNNNNGTC | 1 cut(s) | 338 |
| PsiI | TTATAA | 2 cut(s) | 546, 908 |
| Psp6I | CCWGG | 3 cut(s) | 36, 697, 1324 |
| PspGI | CCWGG | 3 cut(s) | 36, 697, 1324 |
| PspN4I | GGNNCC | 7 cut(s) | 7, 481, 985, 1110, 1119, 1266, 1400 |
| PspPI | GGNCC | 2 cut(s) | 402, 983 |
| PstI | CTGCAG | 2 cut(s) | 234, 610 |
| PsuI | RGATCY | 2 cut(s) | 976, 1162 |
| PteI | GCGCGC | 1 cut(s) | 1780 |
| PvuII | CAGCTG | 1 cut(s) | 960 |
| RsaI | GTAC | 8 cut(s) | 612, 995, 1055, 1110, 1275, 1430, 1581, 1652 |
| RsaNI | GTAC | 8 cut(s) | 611, 994, 1054, 1109, 1274, 1429, 1580, 1651 |
| RseI | CAYNNNNRTG | 1 cut(s) | 179 |
| SalI | GTCGAC | 2 cut(s) | 708, 732 |
| SapI | GCTCTTC | 1 cut(s) | 1460 |
| SaqAI | TTAA | 8 cut(s) | 759, 954, 966, 972, 1133, 1284, 1314, 1722 |
| SatI | GCNGC | 9 cut(s) | 169, 233, 606, 689, 961, 1371, 1527, 1623, 1638 |
| Sau3AI | GATC | 7 cut(s) | 131, 259, 937, 976, 1162, 1415, 1493 |
| Sau96I | GGNCC | 2 cut(s) | 402, 983 |
| ScaI | AGTACT | 1 cut(s) | 995 |
| ScrFI | CCNGG | 3 cut(s) | 38, 699, 1326 |
| SduI | GDGCHC | 4 cut(s) | 8, 273, 382, 1403 |
| SfaNI | GCATC | 3 cut(s) | 637, 776, 1372 |
| SfcI | CTRYAG | 3 cut(s) | 230, 373, 606 |
| SinI | GGWCC | 2 cut(s) | 402, 983 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 179 |
| SmlI | CTYRAG | 4 cut(s) | 383, 941, 989, 1313 |
| SmoI | CTYRAG | 4 cut(s) | 383, 941, 989, 1313 |
| SnaBI | TACGTA | 1 cut(s) | 1230 |
| Sse9I | AATT | 8 cut(s) | 572, 810, 865, 930, 1032, 1038, 1253, 1704 |
| SsiI | CCGC | 4 cut(s) | 10, 169, 394, 1622 |
| SspMI | CTAG | 1 cut(s) | 1215 |
| StyD4I | CCNGG | 3 cut(s) | 36, 697, 1324 |
| StyI | CCWWGG | 4 cut(s) | 405, 513, 1023, 1104 |
| TaaI | ACNGT | 8 cut(s) | 268, 583, 877, 1433, 1486, 1517, 1685, 1693 |
| TaiI | ACGT | 4 cut(s) | 355, 616, 1051, 1232 |
| TaqI | TCGA | 4 cut(s) | 502, 709, 733, 1297 |
| TaqII | GACCGA | 1 cut(s) | 328 |
| TasI | AATT | 8 cut(s) | 572, 810, 865, 930, 1032, 1038, 1253, 1704 |
| TatI | WGTACW | 2 cut(s) | 993, 1053 |
| TauI | GCSGC | 2 cut(s) | 171, 1625 |
| TfiI | GAWTC | 1 cut(s) | 519 |
| Tru1I | TTAA | 8 cut(s) | 759, 954, 966, 972, 1133, 1284, 1314, 1722 |
| Tru9I | TTAA | 8 cut(s) | 759, 954, 966, 972, 1133, 1284, 1314, 1722 |
| TscAI | CASTG | 2 cut(s) | 66, 1653 |
| TseFI | GTSAC | 4 cut(s) | 1148, 1449, 1480, 1610 |
| TseI | GCWGC | 7 cut(s) | 232, 605, 688, 960, 1370, 1526, 1637 |
| Tsp45I | GTSAC | 4 cut(s) | 1148, 1449, 1480, 1610 |
| TspDTI | ATGAA | 2 cut(s) | 163, 848 |
| TspGWI | ACGGA | 1 cut(s) | 575 |
| TspRI | CASTG | 2 cut(s) | 66, 1653 |
| Vha464I | CTTAAG | 1 cut(s) | 1313 |
| VpaK11BI | GGWCC | 2 cut(s) | 402, 983 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 35, 671, 1329 |
| XapI | RAATTY | 2 cut(s) | 865, 1704 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1111 |
| XmiI | GTMKAC | 2 cut(s) | 709, 733 |
| XspI | CTAG | 1 cut(s) | 1215 |
| ZrmI | AGTACT | 1 cut(s) | 995 |