Rw6G009310
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000071
Physical Location & Seq
Reverse (-)
15610784 .. 15615359
4576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009310.1

Sequence Viewer

Length: 2964 bp
ATGGCTGGTGATAACGTCCCATTACCACCACCCAATTCCAGCAAGTCAAATAATCCAACATCTGATATCTCAAATCCATTCTTCACTCACCATTCGGATCATCCTGGATTGGTCTTAGTCTCCAAACCATTAAATGGAGACAATTTCACAGGTTGGAAGAGGGCTATGACAATGGCTCTCAATTCTAAAAACAAGCTCGATTTTGTGAATGGTTCAGTCAAAGTTCCGTCACCCGAAGCCGAACCAGAAGGATATGCAACCTGGTCAAGATGCAATGACATGGTCCATTCATGGATCGTTAACACAGTCAGTTCTGAAATTGCTGACAGTATCATTTACTATCCTACAGCTCATGAGGTATGGGAAGATCTAAGTGAGCGTTTTTCACAAGGCAATGCACCTCGCATATTTGAAATTCAGCGAGACATCGCTTCACTCAGACAAGAACAACAATCAGTTTCTGCATACTACACGAAACTGAAAAGTTTGTGGGATGAATTGGCATCCTATTCAGAAGCATCACAAGGAGCACAGGCAGATCAACAAAAGCTCATGCAATTCTTGATGGGATTAAATGAGACTTACAGTGCAACCCGCGGTCAAATCCTCTTGATGAATCCCCTTCCAAGTGTCAGACAAGCATATGCTGCAGTTGCACAAGAAGAAAAACAACGTTTCGTTGCAGCGGCGGAGTCCAGTTCAAGCACCGCCGCCATGGCTGTCCAGAGTGGTGGCCGGCCGAATCAGTTCGGCAGCAGAGGAGGTCGTGGTGACCGACCGAATCTTCAGACCAGTCGAGATGCCGAACGCGGCTTCACCGCGCGTTTTGGTTCAGGTCGAGGAAGGGGAAGGCCGCACTGTACCTATTGTGGTGATCCAGGACATTGGGTCCAAACATGTTTTCACCTAATTGGTTTTCCTCCAGGTCATCCCAAAGCAAAACAAGGATCGGGAAATTTCCCCAAGAAGCACACTGCCACACCAGTCGTGCCCTCTCCTTCTGCTAACCAGGTTCAAGCGACAGGCATCGTAGAAGAGGAGAATGGATCAGCAGTTACTTTATCTGAGGCCCAATTCAAACAGCTTTTAGCTGCTCTCCAACAAGCCCAAAAGCCCAATCCATATACTGGCTCGAGTTCTAAGGCAAATGCAGTAACGCAGCCAGGTTTGTCTAGAGTAGCTTCCCGCAATTGGATAATTGATAGCGGGGCGACAGATCATATTACTTCGTGCTCTAAATTATTGAATAAAAATAAAAATTGTGCATTACCACCTGTTTTGCTACCTAGTGGACAAAAAGTGCATATTGAAGCCACAGGTTCTTTGCCTCTGAGTACCGTATTTTATCTACATGATGTGCTGTTTGTACCGAAGTTCAAAGATCTGGCTACGAAGAGGACGATTGGTTTGGGTAAAGAACGTAATGGGCTATACTATTTGGTGGCACTAGCGACGGAACGAACCAGGCCTTCCATTACCACTCCTTCCAGATCATCTTGCAACCTTGCCAACGGTACCTGGACCCTTACCCCATTGCCCCCTGGGAAGATCCCTATTGGTTGTAGGTGGGTTTACAAGGTCAAGCTCAAGTCTGATGGTTCGATTGAACGATACAAGGCCAGGTTGGTGGCCAAGGGCTTCACGCAGTTAGCAGGTATTGACTATCAGGACACCTTCTCTCCTACTGCTAAAATTGTTACGGTCCGTTGTCTCCTTGCTTTCGCTGCGTCTCGTGGTTGGTCCCTTTATCAACTCGATGTCAACAATGCCTTTCTCCATGGCAACCTGGATGAAGAAATTTATATGTCTCCACCGCCTGGGCTCCGGAGACAGGGGGAGGAGCATTTGGTTTGTCGGTTGCATAAATCCTTGTACGGTCTGAAACAGGCTTCAAGGCAGTGGTTTTCTAGATTTTCAGAAGTTATTCGGTCTGCTGGCTTTGTGCAATCTAGAGCAGACTACTCTCTTTTTACCAGGAGGCAAGGCAAGTCTTTTACAGTCTTATTGATATATGTTGATGATATATTAATTATTGGCAATGATCCTGTGAGTATAGCTGATGTCAAGAAATTTTTGCATAAAACATTCCGTTTGAAAGATCTTGGCAATTTAAAGTATTTTCTTGGCATTGAAGTGTCAGCATCTAAGAAAGGGATATTTATTTGTCAGCGCAAGTATGCGTTGGAAATTATAAAAGATGCAGGGTTATTGGGTGCTGCTCCTGTTGACACACCCATGGAACGTGGATTAAAGTTGTCAGATAAGACTGAGTTACTCAAAGATCCAGAGAAGTATAGGCGTTTGGTTGGAAGATTGATTTATCTCACAGTGTCAAGGCCGGATATTACATATTCTGTTCATATATTAAGCAGGTTTATGCATCAGCCTCGAAAAGCTCATTGGGAAACAGCTTTGCGAGTGGTACGCTACCTCAAGTCAGCTCCTGGTCAAGGCTTGTTCTTTTCCTCAAATTGTGATCTTCGGCTACGAGCCTTTTGTGACTCAGATTGGGCAGGATGTCCACTTACAAGAAGGTCTACTACAGGGTATTGTGTGTTCCTAGGGCCTTCTTTAATTTCATGGAAGTCAAAACGTCAGAAAACAGTCTCTCTTTCTTCAGCTGAAGCAGAGTATCGTGCTATGACAGGAGCTTGTTGTGAATTAACTTGGCTACGTTATTTGCTTCAAGACTTGGGGATTTTACACCAGGAAACGACATTGCTCTATTGTGACAACAAGGCTGCTTTACATATAGCAGCAAATCCAGTGTTTCATGAACGTACAAGGCATATAGAAATGGATTGTCACTATATCAGGGATAAGATTCAAGATGGTTCAGTGGAGACACGATTTGTGAGTTCTGGAAATCAACTGGCTGATGTTCTAACTAAATCTCTTGGCAAGGAGGATTTCGAACTTATGATTCGCAAGTTGGGTGTGCATGATATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

987

Amino Acids

109.65

Weight (kDa)

9.38

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 30 - 76 8.3e-21 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 56 - 225 1.1e-10 gag-polypeptide of LTR copia-type
Retrotrans_gag PF03732 92 - 193 4.2e-08 Retrotransposon gag protein
Pol_BBD PF22936 398 - 460 1.2e-07 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 504 - 747 6.7e-75 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2192
Acc36I ACCTGC 2 cut(s) 1643, 2361
Acc65I GGTACC 1 cut(s) 1514
AccB1I GGYRCC 1 cut(s) 1514
AccB7I CCANNNNNTGG 3 cut(s) 134, 1127, 1817
AccI GTMKAC 1 cut(s) 2537
AccII CGCG 4 cut(s) 597, 810, 821, 823
AccIII TCCGGA 1 cut(s) 1824
AclI AACGTT 1 cut(s) 673
AclWI GGATC 8 cut(s) 105, 302, 869, 955, 1054, 1543, 2036, 2276
AcoI YGGCCR 3 cut(s) 733, 737, 1629
AcsI RAATTY 4 cut(s) 414, 955, 1797, 2069
AcuI CTGAAG 3 cut(s) 770, 2601, 2643
AfaI GTAC 7 cut(s) 862, 1336, 1368, 1516, 1874, 2424, 2782
AfiI CCNNNNNNNGG 6 cut(s) 134, 1127, 1191, 1817, 1831, 2450
AflIII ACRYGT 1 cut(s) 896
AhdI GACNNNNNGTC 1 cut(s) 887
AjuI GAANNNNNNNTTGG 4 cut(s) 885, 917, 2383, 2415
Alw21I GWGCWC 2 cut(s) 532, 1235
AlwI GGATC 8 cut(s) 105, 302, 869, 955, 1054, 1543, 2036, 2276
AlwNI CAGNNNCTG 2 cut(s) 461, 2444
Ama87I CYCGRG 1 cut(s) 1132
Aor13HI TCCGGA 1 cut(s) 1824
AoxI GGCC 9 cut(s) 733, 737, 851, 1068, 1466, 1617, 1629, 2336, 2564
ApeKI GCWGC 9 cut(s) 647, 683, 753, 1091, 1159, 1724, 2216, 2741, 2756
ApoI RAATTY 4 cut(s) 414, 955, 1797, 2069
ArsI GACNNNNNNTTYG 2 cut(s) 1390, 1422
AseI ATTAAT 1 cut(s) 2027
Asp700I GAANNNNTTC 2 cut(s) 746, 1923
Asp718I GGTACC 1 cut(s) 1514
AspA2I CCTAGG 1 cut(s) 2560
AspLEI GCGC 2 cut(s) 823, 2172
AspS9I GGNCC 7 cut(s) 283, 889, 1069, 1521, 1702, 1740, 2564
AsuHPI GGTGA 7 cut(s) 20, 80, 222, 782, 808, 884, 896
AsuII TTCGAA 1 cut(s) 2913
AvaI CYCGRG 1 cut(s) 1132
AvaII GGWCC 5 cut(s) 283, 889, 1521, 1702, 1740
AvrII CCTAGG 1 cut(s) 2560
BaeGI GKGCMC 1 cut(s) 993
BalI TGGCCA 1 cut(s) 1631
BanI GGYRCC 1 cut(s) 1514
BanII GRGCYC 1 cut(s) 1824
BarI GAAGNNNNNNTAC 2 cut(s) 2524, 2556
BauI CACGAG 1 cut(s) 1731
Bbv12I GWGCWC 2 cut(s) 532, 1235
BbvI GCAGC 9 cut(s) 634, 695, 765, 1078, 1171, 1711, 2203, 2728, 2768
BccI CCATC 3 cut(s) 559, 1589, 2825
BcgI CGANNNNNNTGC 2 cut(s) 2369, 2403
BfaI CTAG 6 cut(s) 1173, 1287, 1448, 1908, 1950, 2561
BfmI CTRYAG 3 cut(s) 345, 648, 2541
BfuAI ACCTGC 2 cut(s) 1643, 2361
BglI GCCNNNNNGGC 1 cut(s) 716
BglII AGATCT 3 cut(s) 367, 1381, 2098
BlnI CCTAGG 1 cut(s) 2560
Bme18I GGWCC 5 cut(s) 283, 889, 1521, 1702, 1740
BmeRI GACNNNNNGTC 1 cut(s) 887
BmeT110I CYCGRG 1 cut(s) 1132
BmgT120I GGNCC 7 cut(s) 283, 889, 1069, 1521, 1702, 1740, 2564
BmiI GGNNCC 5 cut(s) 890, 1516, 1523, 1742, 1823
BmsI GCATC 8 cut(s) 260, 512, 527, 790, 1035, 2150, 2188, 2389
BpmI CTGGAG 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 2913
BpuEI CTTGAG 2 cut(s) 1571, 2417
BsaJI CCNNGG 9 cut(s) 595, 714, 1540, 1541, 1632, 1777, 1817, 2235, 2560
BsaWI WCCGGW 1 cut(s) 1824
BsaXI ACNNNNNCTCC 2 cut(s) 1663, 1693
Bsc4I CCNNNNNNNGG 6 cut(s) 134, 1127, 1191, 1817, 1831, 2450
Bse118I RCCGGY 1 cut(s) 735
Bse1I ACTGG 6 cut(s) 696, 792, 983, 1132, 2765, 2877
Bse3DI GCAATG 5 cut(s) 280, 400, 1532, 2044, 2717
BseAI TCCGGA 1 cut(s) 1824
BseDI CCNNGG 9 cut(s) 595, 714, 1540, 1541, 1632, 1777, 1817, 2235, 2560
BseGI GGATG 6 cut(s) 100, 499, 503, 928, 1795, 2522
BseLI CCNNNNNNNGG 6 cut(s) 134, 1127, 1191, 1817, 1831, 2450
BseMI GCAATG 5 cut(s) 280, 400, 1532, 2044, 2717
BseMII CTCAG 5 cut(s) 451, 1056, 1322, 2259, 2517
BseNI ACTGG 6 cut(s) 696, 792, 983, 1132, 2765, 2877
BseRI GAGGAG 3 cut(s) 774, 1052, 1853
BseSI GKGCMC 1 cut(s) 993
BseX3I CGGCCG 1 cut(s) 737
BseXI GCAGC 9 cut(s) 634, 695, 765, 1078, 1171, 1711, 2203, 2728, 2768
Bsh1236I CGCG 4 cut(s) 597, 810, 821, 823
Bsh1285I CGRYCG 2 cut(s) 740, 779
BshFI GGCC 9 cut(s) 735, 739, 853, 1070, 1468, 1619, 1631, 2338, 2566
BshNI GGYRCC 1 cut(s) 1514
BsiEI CGRYCG 2 cut(s) 740, 779
BsiHKAI GWGCWC 2 cut(s) 532, 1235
BsiHKCI CYCGRG 1 cut(s) 1132
BsiSI CCGG 3 cut(s) 736, 1825, 2339
BslFI GGGAC 2 cut(s) 2, 1726
BslI CCNNNNNNNGG 6 cut(s) 134, 1127, 1191, 1817, 1831, 2450
BsmBI CGTCTC 1 cut(s) 1734
BsmFI GGGAC 2 cut(s) 2, 1726
BsnI GGCC 9 cut(s) 735, 739, 853, 1070, 1468, 1619, 1631, 2338, 2566
BsoBI CYCGRG 1 cut(s) 1132
Bsp119I TTCGAA 1 cut(s) 2913
Bsp1286I GDGCHC 4 cut(s) 532, 993, 1235, 1824
Bsp13I TCCGGA 1 cut(s) 1824
Bsp19I CCATGG 3 cut(s) 714, 1777, 2235
BspANI GGCC 9 cut(s) 735, 739, 853, 1070, 1468, 1619, 1631, 2338, 2566
BspCNI CTCAG 5 cut(s) 450, 1057, 1323, 2260, 2516
BspEI TCCGGA 1 cut(s) 1824
BspFNI CGCG 4 cut(s) 597, 810, 821, 823
BspHI TCATGA 2 cut(s) 352, 2773
BspLI GGNNCC 5 cut(s) 890, 1516, 1523, 1742, 1823
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 1643, 2361
BspPI GGATC 8 cut(s) 105, 302, 869, 955, 1054, 1543, 2036, 2276
BspT104I TTCGAA 1 cut(s) 2913
BspT107I GGYRCC 1 cut(s) 1514
BsrDI GCAATG 5 cut(s) 280, 400, 1532, 2044, 2717
BsrFI RCCGGY 1 cut(s) 735
BsrI ACTGG 6 cut(s) 696, 792, 983, 1132, 2765, 2877
BssAI RCCGGY 1 cut(s) 735
BssECI CCNNGG 9 cut(s) 595, 714, 1540, 1541, 1632, 1777, 1817, 2235, 2560
BssSI CACGAG 1 cut(s) 1731
BssT1I CCWWGG 5 cut(s) 714, 1632, 1777, 2235, 2560
Bst2BI CACGAG 1 cut(s) 1731
Bst6I CTCTTC 3 cut(s) 152, 1029, 1388
BstAPI GCANNNNNTGC 1 cut(s) 647
BstBI TTCGAA 1 cut(s) 2913
BstC8I GCNNGC 2 cut(s) 737, 1936
BstDEI CTNAG 9 cut(s) 115, 371, 437, 1065, 1140, 1331, 2145, 2268, 2503
BstDSI CCRYGG 4 cut(s) 595, 714, 1777, 2235
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 2448
BstF5I GGATG 6 cut(s) 100, 499, 503, 928, 1795, 2522
BstFNI CGCG 4 cut(s) 597, 810, 821, 823
BstHHI GCGC 2 cut(s) 823, 2172
BstMCI CGRYCG 2 cut(s) 740, 779
BstMWI GCNNNNNNNGC 4 cut(s) 647, 653, 716, 1724
BstNSI RCATGY 1 cut(s) 900
BstPI GGTNACC 1 cut(s) 770
BstSFI CTRYAG 3 cut(s) 345, 648, 2541
BstSLI GKGCMC 1 cut(s) 993
BstUI CGCG 4 cut(s) 597, 810, 821, 823
BstV1I GCAGC 9 cut(s) 634, 695, 765, 1078, 1171, 1711, 2203, 2728, 2768
BstX2I RGATCY 5 cut(s) 367, 1381, 1548, 2098, 2281
BstXI CCANNNNNNTGG 3 cut(s) 731, 885, 1627
BstYI RGATCY 5 cut(s) 367, 1381, 1548, 2098, 2281
BstZI CGGCCG 1 cut(s) 737
BsuRI GGCC 9 cut(s) 735, 739, 853, 1070, 1468, 1619, 1631, 2338, 2566
BtgI CCRYGG 4 cut(s) 595, 714, 1777, 2235
BtgZI GCGATG 1 cut(s) 412
BtsCI GGATG 6 cut(s) 100, 499, 503, 928, 1795, 2522
BtsI GCAGTG 2 cut(s) 972, 1904
BtsIMutI CAGTG 7 cut(s) 592, 856, 972, 1904, 2334, 2772, 2844
BveI ACCTGC 2 cut(s) 1643, 2361
Cac8I GCNNGC 2 cut(s) 737, 1936
CaiI CAGNNNCTG 2 cut(s) 461, 2444
CciI TCATGA 2 cut(s) 352, 2773
CfoI GCGC 2 cut(s) 823, 2172
Cfr10I RCCGGY 1 cut(s) 735
Cfr13I GGNCC 7 cut(s) 283, 889, 1069, 1521, 1702, 1740, 2564
Cfr42I CCGCGG 1 cut(s) 598
CpoI CGGWCCG 1 cut(s) 1702
CseI GACGC 1 cut(s) 1716
CsiI ACCWGGT 2 cut(s) 260, 1008
Csp6I GTAC 7 cut(s) 861, 1335, 1367, 1515, 1873, 2423, 2781
CspCI CAANNNNNGTGG 2 cut(s) 1260, 1295
CspI CGGWCCG 1 cut(s) 1702
CviQI GTAC 7 cut(s) 861, 1335, 1367, 1515, 1873, 2423, 2781
DdeI CTNAG 9 cut(s) 115, 371, 437, 1065, 1140, 1331, 2145, 2268, 2503
DraI TTTAAA 1 cut(s) 2112
DriI GACNNNNNGTC 1 cut(s) 887
EaeI YGGCCR 3 cut(s) 733, 737, 1629
EagI CGGCCG 1 cut(s) 737
Eam1104I CTCTTC 3 cut(s) 152, 1029, 1388
Eam1105I GACNNNNNGTC 1 cut(s) 887
EarI CTCTTC 3 cut(s) 152, 1029, 1388
EciI GGCGGA 1 cut(s) 704
EclXI CGGCCG 1 cut(s) 737
Eco130I CCWWGG 5 cut(s) 714, 1632, 1777, 2235, 2560
Eco147I AGGCCT 1 cut(s) 1468
Eco24I GRGCYC 1 cut(s) 1824
Eco32I GATATC 2 cut(s) 67, 2947
Eco47I GGWCC 5 cut(s) 283, 889, 1521, 1702, 1740
Eco52I CGGCCG 1 cut(s) 737
Eco57I CTGAAG 3 cut(s) 770, 2601, 2643
Eco88I CYCGRG 1 cut(s) 1132
Eco91I GGTNACC 1 cut(s) 770
EcoNI CCTNNNNNAGG 1 cut(s) 2448
EcoO109I RGGNCCY 1 cut(s) 2564
EcoO65I GGTNACC 1 cut(s) 770
EcoRV GATATC 2 cut(s) 67, 2947
EcoT14I CCWWGG 5 cut(s) 714, 1632, 1777, 2235, 2560
EcoT22I ATGCAT 1 cut(s) 2382
EcoT38I GRGCYC 1 cut(s) 1824
ErhI CCWWGG 5 cut(s) 714, 1632, 1777, 2235, 2560
Esp3I CGTCTC 1 cut(s) 1734
FaqI GGGAC 2 cut(s) 2, 1726
FauI CCCGC 3 cut(s) 602, 1193, 1199
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 2537
FokI GGATG 6 cut(s) 87, 490, 506, 915, 1802, 2529
FriOI GRGCYC 1 cut(s) 1824
FseI GGCCGGCC 1 cut(s) 739
FspBI CTAG 6 cut(s) 1173, 1287, 1448, 1908, 1950, 2561
GlaI GCGC 2 cut(s) 822, 2171
GsuI CTGGAG 1 cut(s) 906
HaeIII GGCC 9 cut(s) 735, 739, 853, 1070, 1468, 1619, 1631, 2338, 2566
HapII CCGG 3 cut(s) 736, 1825, 2339
HgaI GACGC 1 cut(s) 1716
HhaI GCGC 2 cut(s) 823, 2172
Hin6I GCGC 2 cut(s) 821, 2170
HinP1I GCGC 2 cut(s) 821, 2170
HincII GTYRAC 3 cut(s) 301, 1762, 2227
HindII GTYRAC 3 cut(s) 301, 1762, 2227
HinfI GANTC 7 cut(s) 616, 692, 742, 781, 2501, 2824, 2923
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 3 cut(s) 736, 1825, 2339
HphI GGTGA 7 cut(s) 20, 80, 222, 782, 808, 884, 896
Hpy166II GTNNAC 7 cut(s) 301, 1292, 1573, 1762, 2227, 2522, 2538
Hpy8I GTNNAC 7 cut(s) 301, 1292, 1573, 1762, 2227, 2522, 2538
Hpy99I CGWCG 1 cut(s) 1456
HpyCH4IV ACGT 7 cut(s) 15, 673, 1420, 2242, 2593, 2674, 2779
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 653, 716, 1724
HpyF3I CTNAG 9 cut(s) 115, 371, 437, 1065, 1140, 1331, 2145, 2268, 2503
HpySE526I ACGT 7 cut(s) 15, 673, 1420, 2242, 2593, 2674, 2779
HspAI GCGC 2 cut(s) 821, 2170
Kpn2I TCCGGA 1 cut(s) 1824
KpnI GGTACC 1 cut(s) 1518
KroI GCCGGC 1 cut(s) 735
KroNI GCCGGC 1 cut(s) 737
KspAI GTTAAC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 598
LmnI GCTCC 6 cut(s) 527, 1827, 1840, 2224, 2446, 2648
Lsp1109I GCAGC 9 cut(s) 634, 695, 765, 1078, 1171, 1711, 2203, 2728, 2768
LweI GCATC 8 cut(s) 260, 512, 527, 790, 1035, 2150, 2188, 2389
MabI ACCWGGT 2 cut(s) 260, 1008
MaeI CTAG 6 cut(s) 1173, 1287, 1448, 1908, 1950, 2561
MaeII ACGT 7 cut(s) 15, 673, 1420, 2242, 2593, 2674, 2779
MaeIII GTNAC 9 cut(s) 228, 770, 1054, 1153, 1696, 2271, 2498, 2729, 2804
MfeI CAATTG 1 cut(s) 1189
MflI RGATCY 5 cut(s) 367, 1381, 1548, 2098, 2281
MhlI GDGCHC 4 cut(s) 532, 993, 1235, 1824
MlsI TGGCCA 1 cut(s) 1631
MluNI TGGCCA 1 cut(s) 1631
MlyI GAGTC 2 cut(s) 701, 2495
MmeI TCCRAC 5 cut(s) 80, 134, 1123, 2163, 2287
Mox20I TGGCCA 1 cut(s) 1631
Mph1103I ATGCAT 1 cut(s) 2382
MroI TCCGGA 1 cut(s) 1824
MroNI GCCGGC 1 cut(s) 735
MroXI GAANNNNTTC 2 cut(s) 746, 1923
MscI TGGCCA 1 cut(s) 1631
MseI TTAA 9 cut(s) 131, 300, 572, 2027, 2111, 2249, 2366, 2573, 2663
MslI CAYNNNNRTG 2 cut(s) 2132, 2234
Msp20I TGGCCA 1 cut(s) 1631
MspA1I CMGCKG 3 cut(s) 597, 686, 2621
MspI CCGG 3 cut(s) 736, 1825, 2339
MunI CAATTG 1 cut(s) 1189
MvnI CGCG 4 cut(s) 597, 810, 821, 823
MwoI GCNNNNNNNGC 4 cut(s) 647, 653, 716, 1724
NaeI GCCGGC 1 cut(s) 737
NcoI CCATGG 3 cut(s) 714, 1777, 2235
NdeI CATATG 1 cut(s) 643
NgoMIV GCCGGC 1 cut(s) 735
NlaIV GGNNCC 5 cut(s) 890, 1516, 1523, 1742, 1823
NmuCI GTSAC 5 cut(s) 228, 770, 2498, 2729, 2804
NsiI ATGCAT 1 cut(s) 2382
NspI RCATGY 1 cut(s) 900
NspV TTCGAA 1 cut(s) 2913
PaeR7I CTCGAG 1 cut(s) 1132
PagI TCATGA 2 cut(s) 352, 2773
PasI CCCWGGG 1 cut(s) 1541
PceI AGGCCT 1 cut(s) 1468
PciI ACATGT 1 cut(s) 896
PcsI WCGNNNNNNNCGW 2 cut(s) 772, 1397
PdiI GCCGGC 1 cut(s) 737
PdmI GAANNNNTTC 2 cut(s) 746, 1923
PfeI GAWTC 5 cut(s) 616, 742, 781, 2824, 2923
PflFI GACNNNGTC 1 cut(s) 281
PflMI CCANNNNNTGG 3 cut(s) 134, 1127, 1817
PfoI TCCNGGA 2 cut(s) 103, 877
PleI GAGTC 2 cut(s) 700, 2495
PpsI GAGTC 2 cut(s) 700, 2495
PscI ACATGT 1 cut(s) 896
PshBI ATTAAT 1 cut(s) 2027
PsiI TTATAA 1 cut(s) 2192
Psp1406I AACGTT 1 cut(s) 673
PspEI GGTNACC 1 cut(s) 770
PspN4I GGNNCC 5 cut(s) 890, 1516, 1523, 1742, 1823
PspPI GGNCC 7 cut(s) 283, 889, 1069, 1521, 1702, 1740, 2564
PspXI VCTCGAGB 1 cut(s) 1132
PsrI GAACNNNNNNTAC 2 cut(s) 2540, 2572
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 2 cut(s) 461, 2444
PsuI RGATCY 5 cut(s) 367, 1381, 1548, 2098, 2281
PsyI GACNNNGTC 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 2621
RigI GGCCGGCC 1 cut(s) 739
RsaI GTAC 7 cut(s) 862, 1336, 1368, 1516, 1874, 2424, 2782
RsaNI GTAC 7 cut(s) 861, 1335, 1367, 1515, 1873, 2423, 2781
RseI CAYNNNNRTG 2 cut(s) 2132, 2234
Rsr2I CGGWCCG 1 cut(s) 1702
RsrII CGGWCCG 1 cut(s) 1702
SacII CCGCGG 1 cut(s) 598
SaqAI TTAA 9 cut(s) 131, 300, 572, 2027, 2111, 2249, 2366, 2573, 2663
Sau96I GGNCC 7 cut(s) 283, 889, 1069, 1521, 1702, 1740, 2564
SchI GAGTC 2 cut(s) 701, 2495
SduI GDGCHC 4 cut(s) 532, 993, 1235, 1824
SexAI ACCWGGT 2 cut(s) 260, 1008
SfaNI GCATC 8 cut(s) 260, 512, 527, 790, 1035, 2150, 2188, 2389
SfcI CTRYAG 3 cut(s) 345, 648, 2541
Sfr274I CTCGAG 1 cut(s) 1132
Sfr303I CCGCGG 1 cut(s) 598
SfuI TTCGAA 1 cut(s) 2913
SgrBI CCGCGG 1 cut(s) 598
SinI GGWCC 5 cut(s) 283, 889, 1521, 1702, 1740
SlaI CTCGAG 1 cut(s) 1132
SmiMI CAYNNNNRTG 2 cut(s) 2132, 2234
SmlI CTYRAG 3 cut(s) 1132, 1586, 2432
SmoI CTYRAG 3 cut(s) 1132, 1586, 2432
SseBI AGGCCT 1 cut(s) 1468
SspMI CTAG 6 cut(s) 1173, 1287, 1448, 1908, 1950, 2561
StuI AGGCCT 1 cut(s) 1468
StyI CCWWGG 5 cut(s) 714, 1632, 1777, 2235, 2560
TaiI ACGT 7 cut(s) 18, 676, 1423, 2245, 2596, 2677, 2782
TaqI TCGA 8 cut(s) 198, 796, 838, 1133, 1601, 1755, 2389, 2913
TaqII GACCGA 3 cut(s) 789, 793, 1917
TauI GCSGC 4 cut(s) 689, 713, 813, 856
TfiI GAWTC 5 cut(s) 616, 742, 781, 2824, 2923
Tru1I TTAA 9 cut(s) 131, 300, 572, 2027, 2111, 2249, 2366, 2573, 2663
Tru9I TTAA 9 cut(s) 131, 300, 572, 2027, 2111, 2249, 2366, 2573, 2663
TscAI CASTG 7 cut(s) 592, 863, 979, 1904, 2334, 2772, 2844
TseFI GTSAC 5 cut(s) 228, 770, 2498, 2729, 2804
TseI GCWGC 9 cut(s) 647, 683, 753, 1091, 1159, 1724, 2216, 2741, 2756
Tsp45I GTSAC 5 cut(s) 228, 770, 2498, 2729, 2804
TspDTI ATGAA 8 cut(s) 279, 510, 629, 1806, 2348, 2568, 2762, 2790
TspGWI ACGGA 4 cut(s) 216, 1469, 1694, 2078
TspRI CASTG 7 cut(s) 592, 863, 979, 1904, 2334, 2772, 2844
Tth111I GACNNNGTC 1 cut(s) 281
Van91I CCANNNNNTGG 3 cut(s) 134, 1127, 1817
VpaK11BI GGWCC 5 cut(s) 283, 889, 1521, 1702, 1740
VspI ATTAAT 1 cut(s) 2027
XagI CCTNNNNNAGG 1 cut(s) 2448
XapI RAATTY 4 cut(s) 414, 955, 1797, 2069
XbaI TCTAGA 3 cut(s) 1172, 1907, 1949
XceI RCATGY 1 cut(s) 900
XhoI CTCGAG 1 cut(s) 1132
XmaJI CCTAGG 1 cut(s) 2560
XmiI GTMKAC 1 cut(s) 2537
XmnI GAANNNNTTC 2 cut(s) 746, 1923
XspI CTAG 6 cut(s) 1173, 1287, 1448, 1908, 1950, 2561
Zsp2I ATGCAT 1 cut(s) 2382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.