RchiOBHm_Chr3g0451051

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000071
Physical Location & Seq
Forward (+)
2301005 .. 2304921
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41835

Sequence Viewer

Length: 3738 bp
ATGAATGCCAACAAGAACCCGCAACGAATCCTTCCACCTCTATCATCTGCATCGACATCCACAGTCCCACCTCCTGGATTCTCACAGGCAAAATCCGGAGCTCCTCAACAAAGTTTCTTGCCTCGAAAGAGCTCTTCCATCCGGCCCCGAGGTTTGGTGTGCCAACATTGTGGGGATGCTAGTCACTCTAAGTGGAAGTGTTATGAAATAGTGGGTTATCCAGAATGGTGGGATTTCACCAAGAAGCCTCGGAAGAATATTGGGGGCAAAGTTGCAGTTGCACCTACTTGCTCACTGCCACAAGCAAATGTGACTAAAACAGGTAACCTTGGTAGGTCTCTCAATGTTAATACCAGTGCTAGTAATACTACATGGATAATTGATACAGGTGCATCTGACCATATGACTAATGACCCAGATCTACTCCAATCCATTAAACCATCGGCTCAAGCCATTATCTCTACTGCAGATGGTAGTCCTTCTTCAGTCATTGGAGAGGGTTCTGTCATTTTATCTCCTTCCCTAACCTTAGATACTGTTCTTGTTGTCCCATCTTTAGCTTACAACTTATTATCTGTCAGCCAAATCACTGCGGCTCTATCCTGTCTAGTAATTTTTTGGCCAGCATTTTGTATCTTTCAGGACATCCTAACCCGGAAGATTCTTGGCTATGGTGTAAGAAGGGGCAATTTGTACTACCTCGACTTGACAGACAAGGGAAATCAGAAATTGGGACAGGCACACAAGGCTGGGGGGGTGGAAGATGCAAAAGCAGCAGTGTGGTTATGGCATCGTCGTTTGGGTCATTTGTCTTTTGGATATCTTCGGAAGTTGAAGCCTCATTTGTTTTCAGGTGTGAGTGATTCCGATTTTAAGTGTCATGTTTGTGAACTAGCCAAGAGCCACCGTATTTCGTATTCACCAAGTTTTAATAAAAGTTCAATTCCATTCATGACTGTCCACTCTGATGTTTGGGGACCGGCGAAATATTCTACTATGTCAGGTGCTAAATACTTTGTTACTTTTATTGATGAGTGCACCCGGATGACATGGGTAATGTTGATGAAGAATAAGTGGGAATTATGTCAGGCGTTTCAAGCCTTTCACAAGATGGTTTCTAGCCAGTACCAGAGAAAGATTCAGGTCCTTCAGTCAGATAATGGGGGTGAGTATACAAGTAATGCTATGGCAGAGTTTTGCCAGTCTCAAGGCATTCACCATCAAACTTCTTGTGCTTATACTCCACAGCAAAATGGTCTAGCAGAACGAAAGAATCGTCAGTTGTTAGAGGTTATTCGTGCCTCTCTCTTTGGAATGAATGTACCTCGTGAATATTGGGGCGAAGCATTGAAATCTGCAACTTATCTCATTAACCGAACTCCTTCTAGTGTAATTGATTTTGAGACCCCTCAGCAAAAGTTATGTAGATTGGTCTCTGCTCCTACTTTACCTAATTTGGAGCCTCGAGTCTTTGGGTGCGTAGCATATGTACACATTCCTAAACCTCTACGGAACAAACTTGATCCATGTGCCAAGCGATGTATCTTTGTTGGTTATGCAGAGTTCAAAAAGGGGTACAGGTGTTATGATCCTCAGACGAAGACTCTCCATGTCTCTTTGGATGTATCGTTTCATGAAGGTGAGGCATATTTTACAGGCAAAGCTCAGGAGCCTTCTCTTCAGGGGGAGAAGAGGTATATGGAAGAGAATTCTCAGGATATTTTCTCTGAAATCGAAGAAGTGGAAGAAAGATTGCTACAGCAGGTTCTTTTCAACCCTTCAAACGAAGGCACAACCTCGTCAAATTCCACTCCAGCTGCTCCTGCAGATTCTGAAGGTTTTTTAGACCCACCAAATTGTCTCAACCAGTCAGACAGACTAATGGAACAAACTGAAGAAGAAGCTGATTTGCCCCCTTCTACACCATTGCATGAGGATACTTCCCGGGAAACTCACCCACAGGTAAACCATTGCCAAGAAGTGTCTTTGTATAATCCCATAGAGTCTAATCATTCATCTGTTCCTATAAGACATGTTGAACCTAGATATCCTCAAAGATCCAACAAGGGAATTCCAAGGAAACAGTATGAGCCTGAGCTTAGGGCAAAAGTCAAATATCCAATAGCTAACCATGTGTCTACCCATAGGTTGTCCGAGTCATATGCACTAACTATCAATCAATTATCTACTGTGGCTATTCCTAGAACTGTGCAGGAAGCATTGGATGATCCAAAGTGGAGGACGGCAATGAATGAAGAGATGGAAGCACTTCAGAAGAATGCTACTTGGGAGATTGTACCGTTGCCTCAAGGAAAGAAGACTGTTGGATGTAGATGGATATACACAGTGAAGCTTAAGGCAGATGGGGGCATAGACAGGTACAAAGCAAGACTTGTAGCAAAAGGCTATACCCAGAAGTATGGAGTTGACTATCAGGAGACATTTGCTCCGGTGGCGAAAATTAATACCATTAGAATACTTATGTCTTTGGCCGCCACGTATGATTGGCCATTACAACAATATGATGTGAAGAATGCGTTCCTACACGGAGATTTAGAAGAAGAAGTTTATATGGATATGCCACCAGGAAGTCAGTGCAGGTTATCAGAGGTTGGTAAGGTGTGTAGGTTAAAGAAGTCGTTGTATGGCCTGAAGCAATCACCCAGAGCATGGTTTGGAAGATTCACCAAGGCTATGAAAGCATTTGGCTATCGGCAGAGTAATTCTGATCATACTCTCTTCATCAGGCGCAAAGCTGGTAAGTTGACAGTGCTCATAGTCTATGTAGATGATATGGTGGTAACCGGAGATGATCCAGTAGAAAGGAAAGCATTACAGGATTATTTGGCTACTGAATTTGAGATGAAAGATCTTGGAAAGCTGAAGTATTTCTTGGGAATTGAGGTCGCTAGGTCAAAAGAAGGGATCTCATTGTCCCAACGAAAATATGTCCTTGATTTATTGACAGATACTGGAATGCTTGACTGTCAACCAGCTGATACACCCATGGAGATGAATCACAGACTAGGTGAATGGAAAGATCAAGTTCCCGCAAACAAGTCCAGGTATCAATGTCTTGTTGGTAGATTAATATATTTATCTCATACTAGACCAGACATTGCATACGCAGTCAGTGTGGTTAGTCAGTTTATGCATGCTCCCAGTGAAGACCACATGAATGCAGTTTATCGAATTTTGAGGTATCTAAAGGCTGCTCCAGGGAAAGGATTGTTATTTGCCAAGAAAGACAAGCTGATAGTTGAGGGGTATACTGATGCAGATTGGGCAGGTTCAAAGGTAGATAGAAGATCTACTTCGGGATACTTTACCTTCGTTGGAGGTAATCTCGTGACATGGCGATGTAAGAAACAGAAAGTGGTTGCAAGATCTAGTGCTGAAGCAGAGTTCAGAGGCATGGCACATGGAGTTTGTGAGTTACTATGGATTAGACATCTGTTGAAAGATTTGGGCTTCAAGAGTAGGAAGTCAATGGACTTACATTGTGATAACAAGGCTGCAATCGAAATAGCACATAATCCTGTTCAGCATGACCGTACAAAACATGTGGAGGTAGATCGACACTTCATTAAGGAAAATTTGGACCGAAAAATAATCCGGTTTCCGTTTGTCCAATCAGAAGAGCAGCTAGCAGATATTCTAACCAAAGCAGTTTCAGGAAAAGTATTTAACAGCTCCCTTGACAAGTTGGGCATGATTGACATCTATGCACCAACTTGA

Protein Analysis

1245

Amino Acids

140.25

Weight (kDa)

8.95

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 125 - 198 1e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 230 - 301 1.6e-15 GAG-pre-integrase domain
rve PF00665 319 - 415 1.5e-15 Integrase core domain
SH3_retrovirus PF25597 493 - 551 7.3e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 760 - 1004 5.6e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1753, 2589, 3278
AccB7I CCANNNNNTGG 2 cut(s) 74, 2670
AccI GTMKAC 3 cut(s) 1172, 2138, 3269
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 4 cut(s) 20, 593, 2493, 3051
AclWI GGATC 6 cut(s) 1517, 1583, 2052, 2222, 2807, 2933
AcoI YGGCCR 3 cut(s) 620, 2490, 2507
AcsI RAATTY 6 cut(s) 1708, 1804, 2070, 2855, 3192, 3595
AcuI CTGAAG 9 cut(s) 468, 1133, 1664, 1854, 1914, 2255, 2672, 2903, 3417
AfaI GTAC 8 cut(s) 695, 1127, 1323, 1491, 1577, 2298, 2381, 3556
AfiI CCNNNNNNNGG 4 cut(s) 74, 154, 2670, 3224
AflII CTTAAG 1 cut(s) 2354
AflIII ACRYGT 2 cut(s) 2032, 3562
AjnI CCWGG 4 cut(s) 73, 2584, 3062, 3217
AjuI GAANNNNNNNTTGG 2 cut(s) 2876, 2908
Alw21I GWGCWC 4 cut(s) 103, 134, 1040, 2775
Alw26I GTCTC 7 cut(s) 342, 1209, 1397, 1438, 1618, 1865, 2432
Alw44I GTGCAC 1 cut(s) 1036
AlwI GGATC 6 cut(s) 1517, 1583, 2052, 2222, 2807, 2933
AlwNI CAGNNNCTG 2 cut(s) 1832, 2972
Ama87I CYCGRG 3 cut(s) 147, 1464, 1944
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 5 cut(s) 143, 620, 2490, 2507, 2647
ApaLI GTGCAC 1 cut(s) 1036
ApeKI GCWGC 5 cut(s) 773, 1817, 3212, 3515, 3643
ApoI RAATTY 6 cut(s) 1708, 1804, 2070, 2855, 3192, 3595
ArsI GACNNNNNNTTYG 2 cut(s) 1261, 1293
AseI ATTAAT 2 cut(s) 2463, 3089
Asp700I GAANNNNTTC 4 cut(s) 1381, 2268, 2537, 2888
AspLEI GCGC 1 cut(s) 2751
AspS9I GGNCC 4 cut(s) 144, 977, 1144, 3601
AsuC2I CCSGG 4 cut(s) 655, 1042, 1945, 1946
AsuHPI GGTGA 9 cut(s) 229, 912, 1178, 1208, 1652, 1946, 2652, 2677, 3041
AsuNHI GCTAGC 1 cut(s) 3646
AvaI CYCGRG 3 cut(s) 147, 1464, 1944
AvaII GGWCC 3 cut(s) 977, 1144, 3601
BaeGI GKGCMC 1 cut(s) 1040
BaeI ACNNNNGTAYC 2 cut(s) 1567, 1600
BalI TGGCCA 2 cut(s) 622, 2509
BanII GRGCYC 2 cut(s) 103, 134
BarI GAAGNNNNNNTAC 2 cut(s) 466, 498
BauI CACGAG 2 cut(s) 1326, 3347
BbsI GAAGAC 3 cut(s) 1607, 2324, 3174
Bbv12I GWGCWC 4 cut(s) 103, 134, 1040, 2775
BbvCI CCTCAGC 1 cut(s) 1410
BbvI GCAGC 5 cut(s) 785, 1804, 3199, 3502, 3655
BccI CCATC 9 cut(s) 146, 448, 464, 559, 1105, 1227, 2254, 2328, 2357
BceAI ACGGC 1 cut(s) 2259
BcgI CGANNNNNNTGC 2 cut(s) 2144, 2178
BciT130I CCWGG 4 cut(s) 75, 2586, 3064, 3219
BciVI GTATCC 2 cut(s) 1930, 3314
BclI TGATCA 1 cut(s) 2728
BcnI CCSGG 4 cut(s) 655, 1042, 1945, 1946
BcoDI GTCTC 7 cut(s) 342, 1209, 1397, 1438, 1618, 1865, 2432
BfmI CTRYAG 3 cut(s) 465, 1757, 1824
BfrI CTTAAG 1 cut(s) 2354
BfuAI ACCTGC 3 cut(s) 1753, 2589, 3278
BfuI GTATCC 2 cut(s) 1930, 3314
BglII AGATCT 4 cut(s) 418, 2869, 3308, 3386
BisI GCNGC 7 cut(s) 594, 774, 1818, 2493, 3213, 3516, 3644
BlsI GCNGC 7 cut(s) 595, 775, 1819, 2494, 3214, 3517, 3645
Bme1390I CCNGG 8 cut(s) 75, 655, 1042, 1945, 1946, 2586, 3064, 3219
Bme18I GGWCC 3 cut(s) 977, 1144, 3601
BmeT110I CYCGRG 3 cut(s) 147, 1464, 1944
BmgT120I GGNCC 4 cut(s) 144, 977, 1144, 3601
BmiI GGNNCC 4 cut(s) 146, 978, 1461, 1671
BmrFI CCNGG 8 cut(s) 75, 655, 1042, 1945, 1946, 2586, 3064, 3219
BmrI ACTGGG 1 cut(s) 3156
BmsI GCATC 6 cut(s) 59, 166, 401, 754, 799, 3265
BmtI GCTAGC 1 cut(s) 3650
BmuI ACTGGG 1 cut(s) 3156
BpiI GAAGAC 3 cut(s) 1607, 2324, 3174
BplI GAGNNNNNCTC 2 cut(s) 3330, 3362
BpmI CTGGAG 2 cut(s) 1797, 3201
Bpu10I CCTNAGC 4 cut(s) 1410, 1665, 2094, 2099
BpuEI CTTGAG 3 cut(s) 432, 1191, 2292
BpuMI CCSGG 4 cut(s) 655, 1042, 1945, 1946
BsaAI YACGTR 1 cut(s) 2499
BsaBI GATNNNNATC 1 cut(s) 3719
BsaI GGTCTC 3 cut(s) 342, 1397, 1438
BsaJI CCNNGG 8 cut(s) 148, 248, 328, 1944, 2075, 2688, 3006, 3218
BsaWI WCCGGW 4 cut(s) 95, 2449, 2804, 3615
BsaXI ACNNNNNCTCC 4 cut(s) 1679, 1709, 2431, 2461
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 154, 2670, 3224
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 7 cut(s) 354, 1123, 1201, 1867, 2816, 2977, 3162
Bse3DI GCAATG 4 cut(s) 1925, 1969, 2253, 3117
Bse8I GATNNNNATC 1 cut(s) 3719
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 4 cut(s) 75, 2586, 3064, 3219
BseDI CCNNGG 8 cut(s) 148, 248, 328, 1944, 2075, 2688, 3006, 3218
BseGI GGATG 8 cut(s) 56, 138, 181, 645, 1050, 1627, 2230, 2333
BseJI GATNNNNATC 1 cut(s) 3719
BseLI CCNNNNNNNGG 4 cut(s) 74, 154, 2670, 3224
BseMI GCAATG 4 cut(s) 1925, 1969, 2253, 3117
BseMII CTCAG 5 cut(s) 1424, 1607, 1679, 1727, 2085
BseNI ACTGG 7 cut(s) 354, 1123, 1201, 1867, 2816, 2977, 3162
BseRI GAGGAG 1 cut(s) 93
BseSI GKGCMC 1 cut(s) 1040
BseXI GCAGC 5 cut(s) 785, 1804, 3199, 3502, 3655
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 2 cut(s) 2231, 2617
BshFI GGCC 5 cut(s) 145, 622, 2492, 2509, 2649
BsiHKAI GWGCWC 4 cut(s) 103, 134, 1040, 2775
BsiHKCI CYCGRG 3 cut(s) 147, 1464, 1944
BsiSI CCGG 9 cut(s) 96, 142, 655, 980, 1042, 1945, 2450, 2805, 3616
BslFI GGGAC 5 cut(s) 50, 533, 747, 990, 2920
BslI CCNNNNNNNGG 4 cut(s) 74, 154, 2670, 3224
BsmAI GTCTC 7 cut(s) 342, 1209, 1397, 1438, 1618, 1865, 2432
BsmFI GGGAC 5 cut(s) 50, 533, 747, 990, 2920
BsmI GAATGC 6 cut(s) 10, 1212, 2284, 2539, 2982, 3184
BsnI GGCC 5 cut(s) 145, 622, 2492, 2509, 2649
Bso31I GGTCTC 3 cut(s) 342, 1397, 1438
BsoBI CYCGRG 3 cut(s) 147, 1464, 1944
Bsp1286I GDGCHC 4 cut(s) 103, 134, 1040, 2775
Bsp13I TCCGGA 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 1489
Bsp19I CCATGG 1 cut(s) 3006
BspACI CCGC 4 cut(s) 20, 593, 2493, 3051
BspANI GGCC 5 cut(s) 145, 622, 2492, 2509, 2649
BspCNI CTCAG 5 cut(s) 1423, 1606, 1678, 1726, 2086
BspEI TCCGGA 1 cut(s) 95
BspHI TCATGA 2 cut(s) 951, 1633
BspLI GGNNCC 4 cut(s) 146, 978, 1461, 1671
BspMAI CTGCAG 2 cut(s) 469, 1828
BspMI ACCTGC 3 cut(s) 1753, 2589, 3278
BspOI GCTAGC 1 cut(s) 3650
BspPI GGATC 6 cut(s) 1517, 1583, 2052, 2222, 2807, 2933
BspQI GCTCTTC 2 cut(s) 139, 3633
BspTI CTTAAG 1 cut(s) 2354
BspTNI GGTCTC 3 cut(s) 342, 1397, 1438
BsrDI GCAATG 4 cut(s) 1925, 1969, 2253, 3117
BsrFI RCCGGY 1 cut(s) 979
BsrGI TGTACA 1 cut(s) 1489
BsrI ACTGG 7 cut(s) 354, 1123, 1201, 1867, 2816, 2977, 3162
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 8 cut(s) 148, 248, 328, 1944, 2075, 2688, 3006, 3218
BssNAI GTATAC 2 cut(s) 1173, 3270
BssSI CACGAG 2 cut(s) 1326, 3347
BssT1I CCWWGG 4 cut(s) 328, 2075, 2688, 3006
Bst1107I GTATAC 2 cut(s) 1173, 3270
Bst2BI CACGAG 2 cut(s) 1326, 3347
Bst2UI CCWGG 4 cut(s) 75, 2586, 3064, 3219
Bst6I CTCTTC 7 cut(s) 139, 1683, 1685, 1698, 2250, 2744, 3633
BstAFI CTTAAG 1 cut(s) 2354
BstAUI TGTACA 1 cut(s) 1489
BstBAI YACGTR 1 cut(s) 2499
BstC8I GCNNGC 3 cut(s) 624, 3156, 3648
BstDEI CTNAG 8 cut(s) 189, 529, 1410, 1593, 1665, 1713, 2094, 2099
BstDSI CCRYGG 1 cut(s) 3006
BstEII GGTNACC 2 cut(s) 323, 2800
BstF5I GGATG 8 cut(s) 56, 138, 181, 645, 1050, 1627, 2230, 2333
BstHHI GCGC 1 cut(s) 2751
BstMAI GTCTC 7 cut(s) 342, 1209, 1397, 1438, 1618, 1865, 2432
BstMWI GCNNNNNNNGC 7 cut(s) 746, 773, 1097, 1823, 2453, 2699, 3284
BstNI CCWGG 4 cut(s) 75, 2586, 3064, 3219
BstNSI RCATGY 3 cut(s) 2036, 3158, 3566
BstPI GGTNACC 2 cut(s) 323, 2800
BstSCI CCNGG 8 cut(s) 73, 653, 1040, 1943, 1944, 2584, 3062, 3217
BstSFI CTRYAG 3 cut(s) 465, 1757, 1824
BstSLI GKGCMC 1 cut(s) 1040
BstV1I GCAGC 5 cut(s) 785, 1804, 3199, 3502, 3655
BstV2I GAAGAC 3 cut(s) 1607, 2324, 3174
BstX2I RGATCY 6 cut(s) 418, 2057, 2869, 2925, 3308, 3386
BstXI CCANNNNNNTGG 3 cut(s) 170, 228, 2420
BstYI RGATCY 6 cut(s) 418, 2057, 2869, 2925, 3308, 3386
BstZ17I GTATAC 2 cut(s) 1173, 3270
BsuI GTATCC 2 cut(s) 1930, 3314
BsuRI GGCC 5 cut(s) 145, 622, 2492, 2509, 2649
BtgI CCRYGG 1 cut(s) 3006
BtgZI GCGATG 2 cut(s) 1552, 3373
BtsCI GGATG 8 cut(s) 56, 138, 181, 645, 1050, 1627, 2230, 2333
BtsI GCAGTG 3 cut(s) 293, 588, 783
BtsIMutI CAGTG 9 cut(s) 293, 361, 588, 783, 2352, 2600, 2775, 3139, 3169
BveI ACCTGC 3 cut(s) 1753, 2589, 3278
Cac8I GCNNGC 3 cut(s) 624, 3156, 3648
CaiI CAGNNNCTG 2 cut(s) 1832, 2972
CciI TCATGA 2 cut(s) 951, 1633
CfoI GCGC 1 cut(s) 2751
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 4 cut(s) 144, 977, 1144, 3601
Cfr9I CCCGGG 1 cut(s) 1944
Csp6I GTAC 8 cut(s) 694, 1126, 1322, 1490, 1576, 2297, 2380, 3555
CspCI CAANNNNNGTGG 2 cut(s) 3546, 3581
CviQI GTAC 8 cut(s) 694, 1126, 1322, 1490, 1576, 2297, 2380, 3555
DdeI CTNAG 8 cut(s) 189, 529, 1410, 1593, 1665, 1713, 2094, 2099
EaeI YGGCCR 3 cut(s) 620, 2490, 2507
Eam1104I CTCTTC 7 cut(s) 139, 1683, 1685, 1698, 2250, 2744, 3633
EarI CTCTTC 7 cut(s) 139, 1683, 1685, 1698, 2250, 2744, 3633
Ecl136II GAGCTC 2 cut(s) 101, 132
Eco130I CCWWGG 4 cut(s) 328, 2075, 2688, 3006
Eco24I GRGCYC 2 cut(s) 103, 134
Eco31I GGTCTC 3 cut(s) 342, 1397, 1438
Eco32I GATATC 2 cut(s) 821, 2048
Eco47I GGWCC 3 cut(s) 977, 1144, 3601
Eco53kI GAGCTC 2 cut(s) 101, 132
Eco57I CTGAAG 9 cut(s) 468, 1133, 1664, 1854, 1914, 2255, 2672, 2903, 3417
Eco88I CYCGRG 3 cut(s) 147, 1464, 1944
Eco91I GGTNACC 2 cut(s) 323, 2800
EcoICRI GAGCTC 2 cut(s) 101, 132
EcoO109I RGGNCCY 1 cut(s) 1144
EcoO65I GGTNACC 2 cut(s) 323, 2800
EcoRI GAATTC 2 cut(s) 1708, 2070
EcoRII CCWGG 4 cut(s) 73, 2584, 3062, 3217
EcoRV GATATC 2 cut(s) 821, 2048
EcoT14I CCWWGG 4 cut(s) 328, 2075, 2688, 3006
EcoT22I ATGCAT 1 cut(s) 3156
EcoT38I GRGCYC 2 cut(s) 103, 134
ErhI CCWWGG 4 cut(s) 328, 2075, 2688, 3006
FalI AAGNNNNNCTT 6 cut(s) 2376, 2408, 2876, 2908, 3298, 3330
FaqI GGGAC 5 cut(s) 50, 533, 747, 990, 2920
FauI CCCGC 2 cut(s) 27, 3058
FauNDI CATATG 3 cut(s) 402, 1486, 2161
FbaI TGATCA 1 cut(s) 2728
FblI GTMKAC 3 cut(s) 1172, 2138, 3269
Fnu4HI GCNGC 7 cut(s) 594, 774, 1818, 2493, 3213, 3516, 3644
FokI GGATG 8 cut(s) 43, 125, 188, 632, 1057, 1634, 2237, 2340
FriOI GRGCYC 2 cut(s) 103, 134
Fsp4HI GCNGC 7 cut(s) 594, 774, 1818, 2493, 3213, 3516, 3644
GlaI GCGC 1 cut(s) 2750
GluI GCNGC 7 cut(s) 594, 774, 1818, 2493, 3213, 3516, 3644
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 2 cut(s) 1797, 3201
HaeIII GGCC 5 cut(s) 145, 622, 2492, 2509, 2649
HapII CCGG 9 cut(s) 96, 142, 655, 980, 1042, 1945, 2450, 2805, 3616
HhaI GCGC 1 cut(s) 2751
Hin6I GCGC 1 cut(s) 2749
HinP1I GCGC 1 cut(s) 2749
HincII GTYRAC 3 cut(s) 2428, 2766, 2990
HindII GTYRAC 3 cut(s) 2428, 2766, 2990
HindIII AAGCTT 1 cut(s) 2351
HpaII CCGG 9 cut(s) 96, 142, 655, 980, 1042, 1945, 2450, 2805, 3616
HphI GGTGA 9 cut(s) 229, 912, 1178, 1208, 1652, 1946, 2652, 2677, 3041
Hpy99I CGWCG 1 cut(s) 798
HpyCH4IV ACGT 1 cut(s) 2498
HpyF10VI GCNNNNNNNGC 7 cut(s) 746, 773, 1097, 1823, 2453, 2699, 3284
HpyF3I CTNAG 8 cut(s) 189, 529, 1410, 1593, 1665, 1713, 2094, 2099
HpySE526I ACGT 1 cut(s) 2498
HspAI GCGC 1 cut(s) 2749
Kpn2I TCCGGA 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 2728
LguI GCTCTTC 2 cut(s) 139, 3633
Lsp1109I GCAGC 5 cut(s) 785, 1804, 3199, 3502, 3655
LweI GCATC 6 cut(s) 59, 166, 401, 754, 799, 3265
MaeII ACGT 1 cut(s) 2498
MaeIII GTNAC 7 cut(s) 182, 310, 323, 1018, 2800, 3349, 3435
MflI RGATCY 6 cut(s) 418, 2057, 2869, 2925, 3308, 3386
MhlI GDGCHC 4 cut(s) 103, 134, 1040, 2775
MlsI TGGCCA 2 cut(s) 622, 2509
MluNI TGGCCA 2 cut(s) 622, 2509
MlyI GAGTC 4 cut(s) 1476, 1597, 2012, 2165
MmeI TCCRAC 3 cut(s) 2085, 2305, 3316
Mox20I TGGCCA 2 cut(s) 622, 2509
Mph1103I ATGCAT 1 cut(s) 3156
MroI TCCGGA 1 cut(s) 95
MroXI GAANNNNTTC 4 cut(s) 1381, 2268, 2537, 2888
MscI TGGCCA 2 cut(s) 622, 2509
MslI CAYNNNNRTG 7 cut(s) 885, 966, 1043, 1638, 3011, 3177, 3358
Msp20I TGGCCA 2 cut(s) 622, 2509
MspA1I CMGCKG 2 cut(s) 1817, 2996
MspCI CTTAAG 1 cut(s) 2354
MspI CCGG 9 cut(s) 96, 142, 655, 980, 1042, 1945, 2450, 2805, 3616
MspR9I CCNGG 8 cut(s) 75, 655, 1042, 1945, 1946, 2586, 3064, 3219
Mva1269I GAATGC 6 cut(s) 10, 1212, 2284, 2539, 2982, 3184
MvaI CCWGG 4 cut(s) 75, 2586, 3064, 3219
MwoI GCNNNNNNNGC 7 cut(s) 746, 773, 1097, 1823, 2453, 2699, 3284
NciI CCSGG 4 cut(s) 655, 1042, 1945, 1946
NcoI CCATGG 1 cut(s) 3006
NdeI CATATG 3 cut(s) 402, 1486, 2161
NheI GCTAGC 1 cut(s) 3646
NlaIV GGNNCC 4 cut(s) 146, 978, 1461, 1671
NmuCI GTSAC 3 cut(s) 182, 310, 3349
NsiI ATGCAT 1 cut(s) 3156
NspI RCATGY 3 cut(s) 2036, 3158, 3566
PaeI GCATGC 1 cut(s) 3158
PaeR7I CTCGAG 1 cut(s) 1464
PagI TCATGA 2 cut(s) 951, 1633
PciI ACATGT 2 cut(s) 2032, 3562
PciSI GCTCTTC 2 cut(s) 139, 3633
PcsI WCGNNNNNNNCGW 1 cut(s) 1273
PctI GAATGC 6 cut(s) 10, 1212, 2284, 2539, 2982, 3184
PdmI GAANNNNTTC 4 cut(s) 1381, 2268, 2537, 2888
PfeI GAWTC 9 cut(s) 27, 78, 661, 863, 1138, 1273, 1829, 2682, 3016
PflMI CCANNNNNTGG 2 cut(s) 74, 2670
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 7 cut(s) 595, 775, 1819, 2494, 3214, 3517, 3645
PleI GAGTC 4 cut(s) 1475, 1597, 2011, 2164
PpsI GAGTC 4 cut(s) 1475, 1597, 2011, 2164
Ppu21I YACGTR 1 cut(s) 2499
PpuMI RGGWCCY 1 cut(s) 1144
PscI ACATGT 2 cut(s) 2032, 3562
PshBI ATTAAT 2 cut(s) 2463, 3089
Psp124BI GAGCTC 2 cut(s) 103, 134
Psp5II RGGWCCY 1 cut(s) 1144
Psp6I CCWGG 4 cut(s) 73, 2584, 3062, 3217
PspEI GGTNACC 2 cut(s) 323, 2800
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 4 cut(s) 73, 2584, 3062, 3217
PspN4I GGNNCC 4 cut(s) 146, 978, 1461, 1671
PspPI GGNCC 4 cut(s) 144, 977, 1144, 3601
PspPPI RGGWCCY 1 cut(s) 1144
PspXI VCTCGAGB 1 cut(s) 1464
PstI CTGCAG 2 cut(s) 469, 1828
PstNI CAGNNNCTG 2 cut(s) 1832, 2972
PsuI RGATCY 6 cut(s) 418, 2057, 2869, 2925, 3308, 3386
PvuII CAGCTG 2 cut(s) 1817, 2996
RsaI GTAC 8 cut(s) 695, 1127, 1323, 1491, 1577, 2298, 2381, 3556
RsaNI GTAC 8 cut(s) 694, 1126, 1322, 1490, 1576, 2297, 2380, 3555
RseI CAYNNNNRTG 7 cut(s) 885, 966, 1043, 1638, 3011, 3177, 3358
SacI GAGCTC 2 cut(s) 103, 134
SapI GCTCTTC 2 cut(s) 139, 3633
SatI GCNGC 7 cut(s) 594, 774, 1818, 2493, 3213, 3516, 3644
Sau96I GGNCC 4 cut(s) 144, 977, 1144, 3601
SchI GAGTC 4 cut(s) 1476, 1597, 2012, 2165
ScrFI CCNGG 8 cut(s) 75, 655, 1042, 1945, 1946, 2586, 3064, 3219
SduI GDGCHC 4 cut(s) 103, 134, 1040, 2775
SfaNI GCATC 6 cut(s) 59, 166, 401, 754, 799, 3265
SfcI CTRYAG 3 cut(s) 465, 1757, 1824
Sfr274I CTCGAG 1 cut(s) 1464
SinI GGWCC 3 cut(s) 977, 1144, 3601
SlaI CTCGAG 1 cut(s) 1464
SmaI CCCGGG 1 cut(s) 1946
SmiMI CAYNNNNRTG 7 cut(s) 885, 966, 1043, 1638, 3011, 3177, 3358
SmlI CTYRAG 5 cut(s) 447, 1206, 1464, 2307, 2354
SmoI CTYRAG 5 cut(s) 447, 1206, 1464, 2307, 2354
SphI GCATGC 1 cut(s) 3158
SsiI CCGC 4 cut(s) 20, 593, 2493, 3051
SspI AATATT 3 cut(s) 259, 989, 1334
SstI GAGCTC 2 cut(s) 103, 134
StyD4I CCNGG 8 cut(s) 73, 653, 1040, 1943, 1944, 2584, 3062, 3217
StyI CCWWGG 4 cut(s) 328, 2075, 2688, 3006
TaiI ACGT 1 cut(s) 2501
TaqI TCGA 8 cut(s) 53, 124, 702, 1465, 1734, 3190, 3522, 3577
TaqII GACCGA 1 cut(s) 3618
TatI WGTACW 2 cut(s) 693, 1489
TauI GCSGC 2 cut(s) 596, 2495
TfiI GAWTC 9 cut(s) 27, 78, 661, 863, 1138, 1273, 1829, 2682, 3016
TscAI CASTG 9 cut(s) 300, 361, 595, 783, 2352, 2600, 2775, 3139, 3169
TseFI GTSAC 3 cut(s) 182, 310, 3349
TseI GCWGC 5 cut(s) 773, 1817, 3212, 3515, 3643
Tsp45I GTSAC 3 cut(s) 182, 310, 3349
TspGWI ACGGA 3 cut(s) 1525, 2562, 3612
TspMI CCCGGG 1 cut(s) 1944
TspRI CASTG 9 cut(s) 300, 361, 595, 783, 2352, 2600, 2775, 3139, 3169
Van91I CCANNNNNTGG 2 cut(s) 74, 2670
Vha464I CTTAAG 1 cut(s) 2354
VneI GTGCAC 1 cut(s) 1036
VpaK11BI GGWCC 3 cut(s) 977, 1144, 3601
VspI ATTAAT 2 cut(s) 2463, 3089
XapI RAATTY 6 cut(s) 1708, 1804, 2070, 2855, 3192, 3595
XceI RCATGY 3 cut(s) 2036, 3158, 3566
XhoI CTCGAG 1 cut(s) 1464
XmaI CCCGGG 1 cut(s) 1944
XmiI GTMKAC 3 cut(s) 1172, 2138, 3269
XmnI GAANNNNTTC 4 cut(s) 1381, 2268, 2537, 2888
Zsp2I ATGCAT 1 cut(s) 3156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.