FvH4_2g20531
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000071
Physical Location & Seq
Reverse (-)
17219241 .. 17220619
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20531.t1

Sequence Viewer

Length: 1245 bp
ATGGAATGTATTTTCATAGGTTATCCTGTTGGTCAAAAAGCTTTCAAATTGTATAATCTGTCAACTAAAAAGGTTTTTACAAACAGAGATGTTTATTTTCATGAAAATATTTTTCCATATGCATCCACGAAGCCCAATTCTTCTGTCCCTTCTTCGGCCCATTCAGACAACCCAATTCCTCTTCCCATCCATGGCCCATATCTGATTGACCATGTTTTTGACTCACACTTCTCTCCTTCTCACCACGACAACAACAACAGAGACTCTTCTCTCTTTCCTTCCCCATCTCACGATCTCGCTTCACCATCTCTGCCGCCACCTTCCCTCCTCCGCACCTATTCCCGTCGCAAAATACCTCCACCTCTCTCAGATGCCCAAAACCCCAATCCAATCACATCGGCTTCGGTTCCTTCTGATCCCGTTTCGGCGCCCTCTCCACCACCTTCTCCGGCCGCGGCATCACCATCCAATCCGCAGCTTCACGCCCAACCTAATCTACCTCTTCCTCCGTCTGACCCTACACTCGCCCAACACGATCCGTGTCTGCCTTCACTCGCCCAATCCGACCAGTCTCTTCATCTGCCCGACTCTGAACCTCCTCCTCTCTGTCGCTCTAGTCGCCCGACTGCCCCGCCGGCGAAGTTTCAAGATTACATCTGCTCCAATTTTTGCTCTGACCAATCTCCCTCTGTCATGCAGGTCCGACAAAAGCAAGCAATGCAGTCTGAATTGCAGGCACTTCAAGACAATGGTACATGGACTCTTGCATCCCTTCCCGCTGGCAAGGTTCCCATTGGTTGTCGATGGGTTTACAAGATAAAGCGCAACTCTGAAGGATCGATCGAGAGATATAAGGCGAGACTCGTCGCAAAAGGTTTCACTCAGTTGGAGGGAGTCGATTATCAGGATACTTTTTCGCCCACCGCCAAAATTATCTCTGTTCGTTGTTTATTGGTCTTAGCAGCTGCCCGTGGCTGGTCCCTTCATCAACTTGACATCAACAATGCTTTTCTCCATGGTGATCTGGATGAAGAAATTTACATGTCACTGCCGCCAGGTCTTCAGCGACAGGGGGAGAACCACTTGGTTTGTCGCCTACACAAGTCATTATATGGGTTGAAACAAGCATCCCGTCAATGGTTCGCCAAGTTTTCCGAAGCCATTCGTTCAGCTGGGTATGTGCAATCAAAAGCCGATTACTCATTATTCACTAGAAGGCAAGGCAAGTCTTTCACTGTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3

Protein Analysis

415

Amino Acids

45.74

Weight (kDa)

8.8

Isoelectric Point (pI)

67.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 2 - 44 1.7e-13 Retroviral polymerase SH3-like domain
RVT_2 PF07727 250 - 410 4.3e-50 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 1 cut(s) 455
AciI CCGC 9 cut(s) 314, 331, 453, 455, 473, 632, 777, 924, 1052
AclWI GGATC 3 cut(s) 410, 530, 844
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 1 cut(s) 1035
AcuI CTGAAG 2 cut(s) 852, 1046
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 754
AfiI CCNNNNNNNGG 4 cut(s) 154, 191, 975, 1137
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 4 cut(s) 46, 647, 743, 1120
AjnI CCWGG 1 cut(s) 1054
AluBI AGCT 4 cut(s) 41, 478, 965, 1172
AluI AGCT 4 cut(s) 41, 478, 965, 1172
Alw26I GTCTC 3 cut(s) 255, 576, 853
AlwI GGATC 3 cut(s) 410, 530, 844
AoxI GGCC 3 cut(s) 156, 193, 450
ApeKI GCWGC 3 cut(s) 475, 962, 965
ApoI RAATTY 1 cut(s) 1035
ArsI GACNNNNNNTTYG 2 cut(s) 200, 232
Asp700I GAANNNNTTC 1 cut(s) 1161
AspLEI GCGC 2 cut(s) 430, 825
AspS9I GGNCC 4 cut(s) 157, 194, 700, 978
AsuHPI GGTGA 4 cut(s) 233, 294, 453, 1031
AvaII GGWCC 2 cut(s) 700, 978
BanI GGYRCC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 1052
BbvI GCAGC 3 cut(s) 487, 952, 974
BccI CCATC 5 cut(s) 194, 292, 313, 472, 798
BciT130I CCWGG 1 cut(s) 1056
BciVI GTATCC 1 cut(s) 901
BcoDI GTCTC 3 cut(s) 255, 576, 853
BfaI CTAG 3 cut(s) 615, 1212, 1243
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 688
BfuI GTATCC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 635
BisI GCNGC 7 cut(s) 314, 453, 456, 476, 963, 966, 1052
BlsI GCNGC 7 cut(s) 315, 454, 457, 477, 964, 967, 1053
Bme1390I CCNGG 1 cut(s) 1056
Bme18I GGWCC 2 cut(s) 700, 978
BmgT120I GGNCC 4 cut(s) 157, 194, 700, 978
BmiI GGNNCC 4 cut(s) 408, 429, 789, 980
BmrFI CCNGG 1 cut(s) 1056
BmsI GCATC 5 cut(s) 131, 361, 467, 776, 1136
BpiI GAAGAC 1 cut(s) 1052
Bsa29I ATCGAT 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 4 cut(s) 190, 453, 970, 1015
BsaXI ACNNNNNCTCC 4 cut(s) 309, 339, 644, 674
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 191, 975, 1137
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 1 cut(s) 1056
BseCI ATCGAT 1 cut(s) 839
BseDI CCNNGG 4 cut(s) 190, 453, 970, 1015
BseGI GGATG 6 cut(s) 122, 186, 464, 767, 1033, 1127
BseLI CCNNNNNNNGG 4 cut(s) 154, 191, 975, 1137
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 2 cut(s) 381, 896
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 3 cut(s) 317, 588, 591
BseX3I CGGCCG 1 cut(s) 450
BseXI GCAGC 3 cut(s) 487, 952, 974
BseYI CCCAGC 1 cut(s) 1172
Bsh1236I CGCG 1 cut(s) 455
Bsh1285I CGRYCG 2 cut(s) 453, 843
BshFI GGCC 3 cut(s) 158, 195, 452
BshNI GGYRCC 1 cut(s) 427
BshVI ATCGAT 1 cut(s) 839
BsiEI CGRYCG 2 cut(s) 453, 843
BsiSI CCGG 2 cut(s) 449, 635
BslFI GGGAC 2 cut(s) 131, 964
BslI CCNNNNNNNGG 4 cut(s) 154, 191, 975, 1137
BsmAI GTCTC 3 cut(s) 255, 576, 853
BsmFI GGGAC 2 cut(s) 131, 964
BsnI GGCC 3 cut(s) 158, 195, 452
Bsp143I GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
Bsp19I CCATGG 2 cut(s) 190, 1015
BspACI CCGC 9 cut(s) 314, 331, 453, 455, 473, 632, 777, 924, 1052
BspANI GGCC 3 cut(s) 158, 195, 452
BspCNI CTCAG 2 cut(s) 380, 895
BspDI ATCGAT 1 cut(s) 839
BspFNI CGCG 1 cut(s) 455
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 408, 429, 789, 980
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 3 cut(s) 410, 530, 844
BspT107I GGYRCC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 4 cut(s) 190, 453, 970, 1015
BssMI GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
BssNI GRCGYC 1 cut(s) 428
BssT1I CCWWGG 2 cut(s) 190, 1015
Bst2UI CCWGG 1 cut(s) 1056
Bst4CI ACNGT 1 cut(s) 1237
Bst6I CTCTTC 4 cut(s) 186, 271, 507, 579
BstACI GRCGYC 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 718
BstC8I GCNNGC 4 cut(s) 636, 714, 735, 781
BstDEI CTNAG 3 cut(s) 367, 882, 958
BstDSI CCRYGG 4 cut(s) 190, 453, 970, 1015
BstF5I GGATG 6 cut(s) 122, 186, 464, 767, 1033, 1127
BstFNI CGCG 1 cut(s) 455
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 430, 825
BstKTI GATC 6 cut(s) 295, 418, 538, 839, 843, 1024
BstMAI GTCTC 3 cut(s) 255, 576, 853
BstMBI GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
BstMCI CGRYCG 2 cut(s) 453, 843
BstMWI GCNNNNNNNGC 3 cut(s) 618, 635, 718
BstNI CCWGG 1 cut(s) 1056
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 1 cut(s) 1054
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 3 cut(s) 487, 952, 974
BstV2I GAAGAC 1 cut(s) 1052
BstZI CGGCCG 1 cut(s) 450
Bsu15I ATCGAT 1 cut(s) 839
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 3 cut(s) 158, 195, 452
BsuTUI ATCGAT 1 cut(s) 839
BtgI CCRYGG 4 cut(s) 190, 453, 970, 1015
BtsCI GGATG 6 cut(s) 122, 186, 464, 767, 1033, 1127
BtsI GCAGTG 1 cut(s) 1046
BtsIMutI CAGTG 2 cut(s) 1046, 1233
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 4 cut(s) 636, 714, 735, 781
CciI TCATGA 1 cut(s) 100
CfoI GCGC 2 cut(s) 430, 825
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 4 cut(s) 157, 194, 700, 978
Cfr42I CCGCGG 1 cut(s) 456
ClaI ATCGAT 1 cut(s) 839
Csp6I GTAC 1 cut(s) 753
CspCI CAANNNNNGTGG 2 cut(s) 1070, 1105
CviAII CATG 7 cut(s) 101, 191, 212, 694, 756, 1016, 1042
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 3 cut(s) 367, 882, 958
DinI GGCGCC 1 cut(s) 429
DpnI GATC 6 cut(s) 294, 417, 537, 838, 842, 1023
DpnII GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
EaeI YGGCCR 1 cut(s) 450
EagI CGGCCG 1 cut(s) 450
Eam1104I CTCTTC 4 cut(s) 186, 271, 507, 579
EarI CTCTTC 4 cut(s) 186, 271, 507, 579
EclXI CGGCCG 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 190, 1015
Eco47I GGWCC 2 cut(s) 700, 978
Eco52I CGGCCG 1 cut(s) 450
Eco57I CTGAAG 2 cut(s) 852, 1046
EcoRII CCWGG 1 cut(s) 1054
EcoT14I CCWWGG 2 cut(s) 190, 1015
EcoT22I ATGCAT 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 429
EheI GGCGCC 1 cut(s) 429
ErhI CCWWGG 2 cut(s) 190, 1015
FaeI CATG 7 cut(s) 104, 194, 215, 697, 759, 1019, 1045
FaqI GGGAC 2 cut(s) 131, 964
FatI CATG 7 cut(s) 100, 190, 211, 693, 755, 1015, 1041
FauI CCCGC 2 cut(s) 639, 784
FauNDI CATATG 1 cut(s) 118
Fnu4HI GCNGC 7 cut(s) 314, 453, 456, 476, 963, 966, 1052
FokI GGATG 6 cut(s) 109, 173, 451, 754, 1040, 1114
Fsp4HI GCNGC 7 cut(s) 314, 453, 456, 476, 963, 966, 1052
FspBI CTAG 3 cut(s) 615, 1212, 1243
GlaI GCGC 2 cut(s) 429, 824
GluI GCNGC 7 cut(s) 314, 453, 456, 476, 963, 966, 1052
GsaI CCCAGC 1 cut(s) 1176
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 3 cut(s) 158, 195, 452
HapII CCGG 2 cut(s) 449, 635
HhaI GCGC 2 cut(s) 430, 825
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 7 cut(s) 104, 194, 215, 697, 759, 1019, 1045
Hin6I GCGC 2 cut(s) 428, 823
HinP1I GCGC 2 cut(s) 428, 823
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 6 cut(s) 221, 263, 587, 760, 861, 894
HpaII CCGG 2 cut(s) 449, 635
HphI GGTGA 4 cut(s) 233, 294, 453, 1031
Hpy166II GTNNAC 2 cut(s) 63, 811
Hpy188III TCNNGA 7 cut(s) 101, 290, 647, 743, 844, 905, 1025
Hpy8I GTNNAC 2 cut(s) 63, 811
Hpy99I CGWCG 2 cut(s) 348, 869
HpyCH4III ACNGT 1 cut(s) 1237
HpyCH4V TGCA 6 cut(s) 122, 697, 721, 733, 767, 1183
HpyF10VI GCNNNNNNNGC 3 cut(s) 618, 635, 718
HpyF3I CTNAG 3 cut(s) 367, 882, 958
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 7 cut(s) 104, 194, 215, 697, 759, 1019, 1045
HspAI GCGC 2 cut(s) 428, 823
KasI GGCGCC 1 cut(s) 427
KroI GCCGGC 1 cut(s) 634
KroNI GCCGGC 1 cut(s) 636
KspI CCGCGG 1 cut(s) 456
Kzo9I GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
LmnI GCTCC 1 cut(s) 665
Lsp1109I GCAGC 3 cut(s) 487, 952, 974
LweI GCATC 5 cut(s) 131, 361, 467, 776, 1136
MaeI CTAG 3 cut(s) 615, 1212, 1243
MaeIII GTNAC 1 cut(s) 1044
MalI GATC 6 cut(s) 294, 417, 537, 838, 842, 1023
MboI GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
MboII GAAGA 8 cut(s) 132, 144, 173, 258, 494, 566, 1043, 1052
MluCI AATT 7 cut(s) 47, 136, 174, 664, 728, 930, 1035
Mly113I GGCGCC 1 cut(s) 428
MlyI GAGTC 6 cut(s) 215, 257, 581, 754, 855, 903
MmeI TCCRAC 3 cut(s) 588, 727, 867
Mph1103I ATGCAT 1 cut(s) 124
MreI CGCCGGCG 1 cut(s) 634
MroNI GCCGGC 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 1161
MspA1I CMGCKG 4 cut(s) 455, 779, 965, 1172
MspI CCGG 2 cut(s) 449, 635
MspR9I CCNGG 1 cut(s) 1056
MvaI CCWGG 1 cut(s) 1056
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 618, 635, 718
NaeI GCCGGC 1 cut(s) 636
NarI GGCGCC 1 cut(s) 428
NcoI CCATGG 2 cut(s) 190, 1015
NdeI CATATG 1 cut(s) 118
NdeII GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
NgoMIV GCCGGC 1 cut(s) 634
NlaIII CATG 7 cut(s) 104, 194, 215, 697, 759, 1019, 1045
NlaIV GGNNCC 4 cut(s) 408, 429, 789, 980
NmuCI GTSAC 1 cut(s) 1044
NsiI ATGCAT 1 cut(s) 124
NspI RCATGY 1 cut(s) 1045
PagI TCATGA 1 cut(s) 100
PciI ACATGT 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 2 cut(s) 531, 561
PdiI GCCGGC 1 cut(s) 636
PdmI GAANNNNTTC 1 cut(s) 1161
PkrI GCNGC 7 cut(s) 315, 454, 457, 477, 964, 967, 1053
Ple19I CGATCG 1 cut(s) 843
PleI GAGTC 6 cut(s) 215, 257, 581, 754, 855, 902
PluTI GGCGCC 1 cut(s) 431
PpsI GAGTC 6 cut(s) 215, 257, 581, 754, 855, 902
PscI ACATGT 1 cut(s) 1041
Psp6I CCWGG 1 cut(s) 1054
PspFI CCCAGC 1 cut(s) 1172
PspGI CCWGG 1 cut(s) 1054
PspN4I GGNNCC 4 cut(s) 408, 429, 789, 980
PspPI GGNCC 4 cut(s) 157, 194, 700, 978
PvuI CGATCG 1 cut(s) 843
PvuII CAGCTG 2 cut(s) 965, 1172
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
SacII CCGCGG 1 cut(s) 456
SatI GCNGC 7 cut(s) 314, 453, 456, 476, 963, 966, 1052
Sau3AI GATC 6 cut(s) 292, 415, 535, 836, 840, 1021
Sau96I GGNCC 4 cut(s) 157, 194, 700, 978
SchI GAGTC 6 cut(s) 215, 257, 581, 754, 855, 903
ScrFI CCNGG 1 cut(s) 1056
SfaNI GCATC 5 cut(s) 131, 361, 467, 776, 1136
SfoI GGCGCC 1 cut(s) 429
Sfr303I CCGCGG 1 cut(s) 456
SgrAI CRCCGGYG 1 cut(s) 634
SgrBI CCGCGG 1 cut(s) 456
SinI GGWCC 2 cut(s) 700, 978
Sse9I AATT 7 cut(s) 47, 136, 174, 664, 728, 930, 1035
SsiI CCGC 9 cut(s) 314, 331, 453, 455, 473, 632, 777, 924, 1052
SspDI GGCGCC 1 cut(s) 427
SspI AATATT 1 cut(s) 109
SspMI CTAG 3 cut(s) 615, 1212, 1243
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 2 cut(s) 190, 1015
TaaI ACNGT 1 cut(s) 1237
TaqI TCGA 4 cut(s) 802, 839, 843, 897
TasI AATT 7 cut(s) 47, 136, 174, 664, 728, 930, 1035
TauI GCSGC 4 cut(s) 316, 455, 458, 1054
TscAI CASTG 2 cut(s) 1053, 1240
TseFI GTSAC 1 cut(s) 1044
TseI GCWGC 3 cut(s) 475, 962, 965
Tsp45I GTSAC 1 cut(s) 1044
TspDTI ATGAA 6 cut(s) 4, 89, 117, 566, 974, 1044
TspGWI ACGGA 2 cut(s) 498, 528
TspRI CASTG 2 cut(s) 1053, 1240
VpaK11BI GGWCC 2 cut(s) 700, 978
XapI RAATTY 1 cut(s) 1035
XceI RCATGY 1 cut(s) 1045
XmnI GAANNNNTTC 1 cut(s) 1161
XspI CTAG 3 cut(s) 615, 1212, 1243
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.