Rw2G049790
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000071
Physical Location & Seq
Reverse (-)
77407599 .. 77412272
4674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049790.1

Sequence Viewer

Length: 3144 bp
ATGGCCATCGATGATGCTTCCGGCAAGTCAGCTGCCAACTCTGATTCATCCAAATCAGAGACTGCATCAGATTTTTCAAAAACAGACATCTCGAATCCTTATTTCACTCATCACTCAGATCATCCCGGATTGGTTCTAGTTTCCAAATCCTTGAATGGAGACAACTACTCCGGTTGGAAAAGGGCTATGACCCTAGCTTTAAACTCCAAGAACAAACTCGGTTTCATCGACGGCTCAATCGAAGCTCCTTCGAAAGCAGCTAATCCCGAAGGTTATGCGACTTGGTCGCGGTGTAATGACATGGTACACTCCTGGATAGTCAATACTCTCAATCCAGAAATTGCAGACAAGCAACTCTCTGTCTCAGCCTACTATACAAAATTGAAAAGTTTATGGGATGAATTATCCACATATTCAGACGCATCACATGGAGCACAGGCAGACCAACAGAAATTGATGCAATTCTTGATGGGTTTGAATGAAACTTACAGTGCAACTCGAGGACAAATTCTTTTGATGAATCCTCTTCCAAGTGTTCGTCAAGCATATTCCGCCGTCTCCCAAGAAGAAAAACAGCGGCTACTCAGCGTTTCGCAGTCCTCTGTGGATTCCGGTTCTAGCGCAGCCATGGCAGTAAGGAACGGTAATAAGCCCAATTCCAGTTCGGCTAGAGGAGGAAAGTTCGAGCGATCAGATCGCTCCTATTCACAGCTGGACGCTCGTCCAGATCGCTCCTTCCCAGATCACTCCTCCTCAAATGCAGCAAAACGCCGTTTTGATTCAGATAAGCGCCGCGGATCAGGCAGAGGCAGACCTGAGTGTACCTATTGTGGAGATATGGGCCACTGGGTCCAAACTTGTTATAAGCTGATTGGCTATCCACCAGGCCATCCTAAAGCAAAACAGAATTTTGGCCCGAACACGAATAGTTTTAGAGGAACTCCTTCGGCTAATCAAGTTTCTGAAGTATTCGACAATGATGAAGGAAGACCCGCCGTCTCATTATCAGAAGCCCAACTCAAACAGTTATTATCCCTTCTCAACAAACCGAATGAAGGCTCTACCTCCAAAGCAAATGCGGTAACAAAACCAGGTTTGTCTAAAGTCGCATCCCGCAATTGGATCATTGACAGCGGGGCGACAGATCATATTACTTCATCATCTCAATTATTGCATAAGAATAAAAATTGTTCGTTACCGCCCGTATTATTGCCTAGTGGAGAAAAAGTGAATATTGTTGCAAAAGGATCTTTGCCTCTGAATACAATCTATTATCTACATGATGTGTTATCTGTGCCTACATTCAAAGTTGATCTAATGTCGGATCTGGCTACGAAGAGGACGATTGGTTTGGGTAAACAACGTGGCGGGCTGTACTACCTAGTGGAACTAGCGTCAGAGAAGTCAATCCCCAACTCTTCCTCATCCACAAATCGACCACTCTGCAATCTTACCACCTCATCCACCAACCTCTGGCATAATCGCTTAGGCCATGTGTCATCCTCTTCGCCGCCGCTTTCCCCTCGGCCCCCCATCCTCCGCACTTACTCTCGCCGTCCTCCCACCCACGCCCCACCCGCTCCCGCGCTTTCACCTTCTCTTCCTCTCCCTTCATCGTCTTCCACACCGGAATCCCCACCGCACCCGGTTCTTGATTCGCCCCCGCACCATCTTGTTGATTCGCCACCGCACCCACTTCTTGGTTCGCCGCCTTCATCTGACCCCGACCCTGTTCTGGAACCCATCCCTGCACCCGCTCTCGAACCCCCTCCCGTTCGTCGCTCCGGCCGTCCGACTGCCGTTCCTGTCAAGCTCAATGATTATGTCTGCTCCCATGTTTGCTCCGACCAATCGCTCTCCCTCGTCCCAGGTCCAACGATCGGTACCCGCTATCCCCTAGCTCATTATGTTTCTTATCACCGATATGGGCCTGCCTATCGGTCGTTTATTGCTCACATTCATGCGGTCACAGAACCTCAGTCTTATTCGGAGGCCGTTGCTCTTCCAGAATGGCGTGCTGCTATGGATTCTGAATTGGAGGCGTTGCAGGCTAATGGCACTTGGTCGCTCACTTCTCTTCCCGCCGGCAAAACCCCCATTGGTTGCCGTTGGGTCTACAAGATCAAGCACAAGTCAGATGGCTCTGTCGAGCGGTACAAAGCACGGTTAGTGGCTAAAGGCTTCACCCAGTTAGAGGGTATCATCTCGGTTCGTTGTTTACTTGCCTTGGCTGCTTCTCGTGGTTGGTCACTACATCAGTTAGACGTGAACAATGCCTTCCTTCATGGTGACCTCGATGAAGAAATTTACATGTCTCCGCAGTGGTTTTCTAAATTCTCCGAAGCTATTCGTTCAGCTGGTTTTGTACAATCCCGAGCTGACTACTCACTCTTCATCAGAAAGCAAGGCAAGTCTTTTACTGCCCTTTTGATATATGTTGACGACATTCTAATTACTGGCAATGATCCTATAAGTATAGCTGATATCAAGAAGTTCTTGCATAATAAATTTCGCCTTAAAGACCTTGGTAACTTGAAATATTTTCTTGGTATTGAGATTTCTGCATCTAGGAAAGGCATTTTTATTTCTCAACGCAAATATGCATTGGAAATCATTAAGGATGCAGGGTTATTAGGGGCAGCTCCCATCGATACACCCATGGAACGTGGGTTAAAATTATCTGATAAGAGTGAGCCACTCAAAGATCCAGGGCGGTCTACTACAGGGTACTGTGTGTTCTTAGGTCCTTCTTTGATTTCATGGCGGTCAAAGCGCCAGAAAACAGTCTCCCTTTCTTCAGCTGAAGCAGAGTATCGTGCAATGACAGGAGCTTGTTGTGAATTGACATGGCTTCGTTATTTACTTAGAGATTTGGGTGTTTTGCATCGAGAGACTGCATTGTTGTATTGTGATAATAAGGCTGCATTACACATCGCAGCAAATCCAGTGTTTCATGAACGTACAAGGCATATAGAAATGGATTGCCATTATATCAGGGACAAGATTCAAGATGGTTCGGTGGAGACAAGGTTTGTTAGTTCTACAAATCAGCTGGCTGACATTTTGACCAAAGCTCTTGGCAAAGAGATCTTCATACCAATGATTCGCAAGTTGGGAGTGCAAGACATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

1047

Amino Acids

114.85

Weight (kDa)

9.15

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 37 - 83 8e-24 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 374 - 441 2.4e-08 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 685 - 773 1.4e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 774 - 888 2.9e-22 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
Acc65I GGTACC 1 cut(s) 1883
AccB1I GGYRCC 1 cut(s) 1883
AccB7I CCANNNNNTGG 2 cut(s) 1473, 1700
AccBSI CCGCTC 3 cut(s) 1580, 1757, 2152
AccI GTMKAC 2 cut(s) 2115, 2717
AccII CGCG 3 cut(s) 289, 795, 1586
AclWI GGATC 6 cut(s) 805, 1130, 1255, 1332, 2459, 2699
AcoI YGGCCR 2 cut(s) 3, 1786
AcsI RAATTY 5 cut(s) 507, 907, 2304, 2333, 2507
AcuI CTGAAG 3 cut(s) 984, 2781, 2823
AfaI GTAC 8 cut(s) 306, 823, 1376, 1885, 2156, 2367, 2729, 2962
AfiI CCNNNNNNNGG 7 cut(s) 1055, 1119, 1473, 1700, 1735, 1880, 2194
AflIII ACRYGT 1 cut(s) 2310
AgsI TTSAA 7 cut(s) 78, 154, 385, 478, 1306, 2536, 3008
AhdI GACNNNNNGTC 1 cut(s) 995
AjiI CACGTC 1 cut(s) 2266
AjnI CCWGG 5 cut(s) 311, 883, 1090, 1867, 2707
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 9 cut(s) 53, 153, 367, 562, 1003, 2319, 2791, 2885, 3017
AlwI GGATC 6 cut(s) 805, 1130, 1255, 1332, 2459, 2699
AlwNI CAGNNNCTG 1 cut(s) 62
Ama87I CYCGRG 2 cut(s) 498, 2373
AoxI GGCC 9 cut(s) 3, 841, 886, 913, 1489, 1526, 1786, 1928, 1992
ApeKI GCWGC 9 cut(s) 32, 257, 623, 761, 2018, 2231, 2639, 2921, 2936
ApoI RAATTY 5 cut(s) 507, 907, 2304, 2333, 2507
ArsI GACNNNNNNTTYG 2 cut(s) 1333, 1365
Asp700I GAANNNNTTC 2 cut(s) 943, 2346
Asp718I GGTACC 1 cut(s) 1883
AspLEI GCGC 4 cut(s) 623, 792, 1588, 2775
AspS9I GGNCC 7 cut(s) 841, 850, 914, 1527, 1871, 1928, 2744
AsuC2I CCSGG 2 cut(s) 126, 1646
AsuHPI GGTGA 4 cut(s) 1584, 1910, 2176, 2300
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 2 cut(s) 498, 2373
AvaII GGWCC 3 cut(s) 850, 1871, 2744
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1883
BauI CACGAG 1 cut(s) 2238
BbsI GAAGAC 2 cut(s) 994, 1611
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 9 cut(s) 19, 269, 635, 773, 2005, 2218, 2651, 2908, 2948
BccI CCATC 9 cut(s) 14, 463, 897, 1541, 1677, 1751, 2132, 2654, 3005
BceAI ACGGC 9 cut(s) 247, 539, 756, 980, 1539, 1773, 1784, 1979, 2091
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 1425, 1459
BciT130I CCWGG 5 cut(s) 313, 885, 1092, 1869, 2709
BcnI CCSGG 2 cut(s) 126, 1646
BcoDI GTCTC 9 cut(s) 53, 153, 367, 562, 1003, 2319, 2791, 2885, 3017
BfaI CTAG 9 cut(s) 137, 194, 618, 669, 1215, 1382, 1391, 1898, 2568
BfmI CTRYAG 1 cut(s) 2721
BfoI RGCGCY 2 cut(s) 793, 2776
BglII AGATCT 1 cut(s) 3087
Bme1390I CCNGG 7 cut(s) 126, 313, 885, 1092, 1646, 1869, 2709
Bme18I GGWCC 3 cut(s) 850, 1871, 2744
BmeRI GACNNNNNGTC 1 cut(s) 995
BmeT110I CYCGRG 2 cut(s) 498, 2373
BmgBI CACGTC 1 cut(s) 2266
BmgT120I GGNCC 7 cut(s) 841, 850, 914, 1527, 1871, 1928, 2744
BmiI GGNNCC 4 cut(s) 851, 1529, 1740, 1885
BmrFI CCNGG 7 cut(s) 126, 313, 885, 1092, 1646, 1869, 2709
BmrI ACTGGG 2 cut(s) 856, 2182
BmsI GCATC 8 cut(s) 4, 74, 431, 447, 1118, 2573, 2611, 2893
BmuI ACTGGG 2 cut(s) 856, 2182
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 2 cut(s) 994, 1611
Bpu10I CCTNAGC 1 cut(s) 1486
Bpu14I TTCGAA 1 cut(s) 251
BpuMI CCSGG 2 cut(s) 126, 1646
Bsa29I ATCGAT 2 cut(s) 9, 2649
BsaJI CCNNGG 8 cut(s) 627, 793, 1523, 1867, 2226, 2524, 2658, 2708
BsaWI WCCGGW 3 cut(s) 170, 611, 1627
BsaXI ACNNNNNCTCC 6 cut(s) 152, 182, 1212, 1242, 3014, 3044
Bsc4I CCNNNNNNNGG 7 cut(s) 1055, 1119, 1473, 1700, 1735, 1880, 2194
Bse118I RCCGGY 1 cut(s) 2084
Bse1I ACTGG 5 cut(s) 660, 851, 2188, 2461, 2945
Bse3DI GCAATG 2 cut(s) 2467, 2826
BseBI CCWGG 5 cut(s) 313, 885, 1092, 1869, 2709
BseCI ATCGAT 2 cut(s) 9, 2649
BseDI CCNNGG 8 cut(s) 627, 793, 1523, 1867, 2226, 2524, 2658, 2708
BseLI CCNNNNNNNGG 7 cut(s) 1055, 1119, 1473, 1700, 1735, 1880, 2194
BseMI GCAATG 2 cut(s) 2467, 2826
BseMII CTCAG 5 cut(s) 129, 378, 598, 807, 1991
BseNI ACTGG 5 cut(s) 660, 851, 2188, 2461, 2945
BseRI GAGGAG 3 cut(s) 687, 739, 742
BseX3I CGGCCG 1 cut(s) 1786
BseXI GCAGC 9 cut(s) 19, 269, 635, 773, 2005, 2218, 2651, 2908, 2948
BsgI GTGCAG 1 cut(s) 1734
Bsh1236I CGCG 3 cut(s) 289, 795, 1586
Bsh1285I CGRYCG 3 cut(s) 1789, 1881, 1943
BshFI GGCC 9 cut(s) 5, 843, 888, 915, 1491, 1528, 1788, 1930, 1994
BshNI GGYRCC 1 cut(s) 1883
BshVI ATCGAT 2 cut(s) 9, 2649
BsiEI CGRYCG 3 cut(s) 1789, 1881, 1943
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 2 cut(s) 498, 2373
BsiSI CCGG 8 cut(s) 21, 126, 171, 612, 1628, 1646, 1785, 2085
BslFI GGGAC 2 cut(s) 1850, 3011
BslI CCNNNNNNNGG 7 cut(s) 1055, 1119, 1473, 1700, 1735, 1880, 2194
BsmAI GTCTC 9 cut(s) 53, 153, 367, 562, 1003, 2319, 2791, 2885, 3017
BsmBI CGTCTC 2 cut(s) 562, 1003
BsmFI GGGAC 2 cut(s) 1850, 3011
BsnI GGCC 9 cut(s) 5, 843, 888, 915, 1491, 1528, 1788, 1930, 1994
BsoBI CYCGRG 2 cut(s) 498, 2373
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 2365
Bsp19I CCATGG 2 cut(s) 627, 2658
BspANI GGCC 9 cut(s) 5, 843, 888, 915, 1491, 1528, 1788, 1930, 1994
BspCNI CTCAG 5 cut(s) 128, 377, 597, 808, 1990
BspDI ATCGAT 2 cut(s) 9, 2649
BspFNI CGCG 3 cut(s) 289, 795, 1586
BspHI TCATGA 1 cut(s) 2953
BspLI GGNNCC 4 cut(s) 851, 1529, 1740, 1885
BspPI GGATC 6 cut(s) 805, 1130, 1255, 1332, 2459, 2699
BspQI GCTCTTC 1 cut(s) 2007
BspT104I TTCGAA 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 1883
BsrBI CCGCTC 3 cut(s) 1580, 1757, 2152
BsrDI GCAATG 2 cut(s) 2467, 2826
BsrFI RCCGGY 1 cut(s) 2084
BsrGI TGTACA 1 cut(s) 2365
BsrI ACTGG 5 cut(s) 660, 851, 2188, 2461, 2945
BssAI RCCGGY 1 cut(s) 2084
BssECI CCNNGG 8 cut(s) 627, 793, 1523, 1867, 2226, 2524, 2658, 2708
BssSI CACGAG 1 cut(s) 2238
BssT1I CCWWGG 4 cut(s) 627, 2226, 2524, 2658
Bst2BI CACGAG 1 cut(s) 2238
Bst2UI CCWGG 5 cut(s) 313, 885, 1092, 1869, 2709
Bst4CI ACNGT 6 cut(s) 491, 644, 1026, 2166, 2732, 2785
Bst6I CTCTTC 8 cut(s) 531, 1331, 1423, 1510, 1605, 2007, 2082, 2396
BstAUI TGTACA 1 cut(s) 2365
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 6 cut(s) 1370, 1932, 2016, 2049, 2086, 3054
BstDEI CTNAG 8 cut(s) 115, 364, 584, 816, 1486, 1977, 2740, 2864
BstDSI CCRYGG 3 cut(s) 627, 793, 2658
BstEII GGTNACC 1 cut(s) 2288
BstFNI CGCG 3 cut(s) 289, 795, 1586
BstH2I RGCGCY 2 cut(s) 793, 2776
BstHHI GCGC 4 cut(s) 623, 792, 1588, 2775
BstMAI GTCTC 9 cut(s) 53, 153, 367, 562, 1003, 2319, 2791, 2885, 3017
BstMCI CGRYCG 3 cut(s) 1789, 1881, 1943
BstMWI GCNNNNNNNGC 7 cut(s) 551, 629, 801, 1577, 2048, 2231, 2770
BstNI CCWGG 5 cut(s) 313, 885, 1092, 1869, 2709
BstNSI RCATGY 1 cut(s) 2314
BstPAI GACNNNNGTC 1 cut(s) 720
BstPI GGTNACC 1 cut(s) 2288
BstSCI CCNGG 7 cut(s) 124, 311, 883, 1090, 1644, 1867, 2707
BstSFI CTRYAG 1 cut(s) 2721
BstUI CGCG 3 cut(s) 289, 795, 1586
BstV1I GCAGC 9 cut(s) 19, 269, 635, 773, 2005, 2218, 2651, 2908, 2948
BstV2I GAAGAC 2 cut(s) 994, 1611
BstX2I RGATCY 4 cut(s) 1247, 1324, 2704, 3087
BstYI RGATCY 4 cut(s) 1247, 1324, 2704, 3087
BstZI CGGCCG 1 cut(s) 1786
Bsu15I ATCGAT 2 cut(s) 9, 2649
BsuRI GGCC 9 cut(s) 5, 843, 888, 915, 1491, 1528, 1788, 1930, 1994
BsuTUI ATCGAT 2 cut(s) 9, 2649
BtgI CCRYGG 3 cut(s) 627, 793, 2658
BtgZI GCGATG 1 cut(s) 2917
BtrI CACGTC 1 cut(s) 2266
BtsI GCAGTG 1 cut(s) 2327
BtsIMutI CAGTG 4 cut(s) 496, 844, 2327, 2952
Cac8I GCNNGC 6 cut(s) 1370, 1932, 2016, 2049, 2086, 3054
CaiI CAGNNNCTG 1 cut(s) 62
CciI TCATGA 1 cut(s) 2953
CfoI GCGC 4 cut(s) 623, 792, 1588, 2775
Cfr10I RCCGGY 1 cut(s) 2084
Cfr13I GGNCC 7 cut(s) 841, 850, 914, 1527, 1871, 1928, 2744
Cfr42I CCGCGG 1 cut(s) 796
ClaI ATCGAT 2 cut(s) 9, 2649
CseI GACGC 3 cut(s) 428, 725, 1383
CsiI ACCWGGT 1 cut(s) 1090
Csp6I GTAC 8 cut(s) 305, 822, 1375, 1884, 2155, 2366, 2728, 2961
CviQI GTAC 8 cut(s) 305, 822, 1375, 1884, 2155, 2366, 2728, 2961
DdeI CTNAG 8 cut(s) 115, 364, 584, 816, 1486, 1977, 2740, 2864
DraI TTTAAA 1 cut(s) 201
DriI GACNNNNNGTC 1 cut(s) 995
EaeI YGGCCR 2 cut(s) 3, 1786
EagI CGGCCG 1 cut(s) 1786
Eam1104I CTCTTC 8 cut(s) 531, 1331, 1423, 1510, 1605, 2007, 2082, 2396
Eam1105I GACNNNNNGTC 1 cut(s) 995
EarI CTCTTC 8 cut(s) 531, 1331, 1423, 1510, 1605, 2007, 2082, 2396
EciI GGCGGA 1 cut(s) 541
EclXI CGGCCG 1 cut(s) 1786
Eco130I CCWWGG 4 cut(s) 627, 2226, 2524, 2658
Eco32I GATATC 1 cut(s) 2485
Eco47I GGWCC 3 cut(s) 850, 1871, 2744
Eco52I CGGCCG 1 cut(s) 1786
Eco57I CTGAAG 3 cut(s) 984, 2781, 2823
Eco88I CYCGRG 2 cut(s) 498, 2373
Eco91I GGTNACC 1 cut(s) 2288
EcoO109I RGGNCCY 1 cut(s) 2744
EcoO65I GGTNACC 1 cut(s) 2288
EcoRII CCWGG 5 cut(s) 311, 883, 1090, 1867, 2707
EcoRV GATATC 1 cut(s) 2485
EcoT14I CCWWGG 4 cut(s) 627, 2226, 2524, 2658
EcoT22I ATGCAT 1 cut(s) 2605
ErhI CCWWGG 4 cut(s) 627, 2226, 2524, 2658
Esp3I CGTCTC 2 cut(s) 562, 1003
FalI AAGNNNNNCTT 2 cut(s) 2480, 2512
FaqI GGGAC 2 cut(s) 1850, 3011
FblI GTMKAC 2 cut(s) 2115, 2717
FspBI CTAG 9 cut(s) 137, 194, 618, 669, 1215, 1382, 1391, 1898, 2568
GlaI GCGC 4 cut(s) 622, 791, 1587, 2774
HaeII RGCGCY 2 cut(s) 793, 2776
HaeIII GGCC 9 cut(s) 5, 843, 888, 915, 1491, 1528, 1788, 1930, 1994
HapII CCGG 8 cut(s) 21, 126, 171, 612, 1628, 1646, 1785, 2085
HgaI GACGC 3 cut(s) 428, 725, 1383
HhaI GCGC 4 cut(s) 623, 792, 1588, 2775
Hin6I GCGC 4 cut(s) 621, 790, 1586, 2773
HinP1I GCGC 4 cut(s) 621, 790, 1586, 2773
HincII GTYRAC 1 cut(s) 2440
HindII GTYRAC 1 cut(s) 2440
HpaII CCGG 8 cut(s) 21, 126, 171, 612, 1628, 1646, 1785, 2085
HphI GGTGA 4 cut(s) 1584, 1910, 2176, 2300
Hpy166II GTNNAC 8 cut(s) 307, 822, 1358, 2116, 2219, 2269, 2440, 2718
Hpy8I GTNNAC 8 cut(s) 307, 822, 1358, 2116, 2219, 2269, 2440, 2718
Hpy99I CGWCG 2 cut(s) 233, 1782
HpyCH4III ACNGT 6 cut(s) 491, 644, 1026, 2166, 2732, 2785
HpyCH4IV ACGT 4 cut(s) 1363, 2265, 2665, 2959
HpyF10VI GCNNNNNNNGC 7 cut(s) 551, 629, 801, 1577, 2048, 2231, 2770
HpyF3I CTNAG 8 cut(s) 115, 364, 584, 816, 1486, 1977, 2740, 2864
HpySE526I ACGT 4 cut(s) 1363, 2265, 2665, 2959
HspAI GCGC 4 cut(s) 621, 790, 1586, 2773
KpnI GGTACC 1 cut(s) 1887
KroI GCCGGC 1 cut(s) 2084
KroNI GCCGGC 1 cut(s) 2086
KspI CCGCGG 1 cut(s) 796
LguI GCTCTTC 1 cut(s) 2007
Lsp1109I GCAGC 9 cut(s) 19, 269, 635, 773, 2005, 2218, 2651, 2908, 2948
LweI GCATC 8 cut(s) 4, 74, 431, 447, 1118, 2573, 2611, 2893
MabI ACCWGGT 1 cut(s) 1090
MaeI CTAG 9 cut(s) 137, 194, 618, 669, 1215, 1382, 1391, 1898, 2568
MaeII ACGT 4 cut(s) 1363, 2265, 2665, 2959
MaeIII GTNAC 6 cut(s) 1081, 1194, 1966, 2247, 2288, 2528
MbiI CCGCTC 3 cut(s) 1580, 1757, 2152
MfeI CAATTG 1 cut(s) 1117
MflI RGATCY 4 cut(s) 1247, 1324, 2704, 3087
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 5 cut(s) 155, 1302, 1817, 1869, 1898
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 2605
MroNI GCCGGC 1 cut(s) 2084
MroXI GAANNNNTTC 2 cut(s) 943, 2346
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 200, 2517, 2616, 2672
MslI CAYNNNNRTG 3 cut(s) 1923, 1959, 3098
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 8 cut(s) 32, 577, 712, 795, 1134, 2357, 2801, 3052
MspI CCGG 8 cut(s) 21, 126, 171, 612, 1628, 1646, 1785, 2085
MspR9I CCNGG 7 cut(s) 126, 313, 885, 1092, 1646, 1869, 2709
MunI CAATTG 1 cut(s) 1117
MvaI CCWGG 5 cut(s) 313, 885, 1092, 1869, 2709
MvnI CGCG 3 cut(s) 289, 795, 1586
MwoI GCNNNNNNNGC 7 cut(s) 551, 629, 801, 1577, 2048, 2231, 2770
NaeI GCCGGC 1 cut(s) 2086
NciI CCSGG 2 cut(s) 126, 1646
NcoI CCATGG 2 cut(s) 627, 2658
NgoMIV GCCGGC 1 cut(s) 2084
NlaIV GGNNCC 4 cut(s) 851, 1529, 1740, 1885
NmeAIII GCCGAG 1 cut(s) 1504
NmuCI GTSAC 3 cut(s) 1966, 2247, 2288
NsiI ATGCAT 1 cut(s) 2605
NspI RCATGY 1 cut(s) 2314
NspV TTCGAA 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 498
PagI TCATGA 1 cut(s) 2953
PciI ACATGT 1 cut(s) 2310
PciSI GCTCTTC 1 cut(s) 2007
PcsI WCGNNNNNNNCGW 4 cut(s) 225, 237, 1340, 1786
PdiI GCCGGC 1 cut(s) 2086
PdmI GAANNNNTTC 2 cut(s) 943, 2346
PflFI GACNNNGTC 1 cut(s) 283
PflMI CCANNNNNTGG 2 cut(s) 1473, 1700
PfoI TCCNGGA 2 cut(s) 124, 311
Ple19I CGATCG 1 cut(s) 1881
PpuMI RGGWCCY 1 cut(s) 2744
PscI ACATGT 1 cut(s) 2310
PshAI GACNNNNGTC 1 cut(s) 720
PsiI TTATAA 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 2744
Psp6I CCWGG 5 cut(s) 311, 883, 1090, 1867, 2707
PspEI GGTNACC 1 cut(s) 2288
PspGI CCWGG 5 cut(s) 311, 883, 1090, 1867, 2707
PspN4I GGNNCC 4 cut(s) 851, 1529, 1740, 1885
PspPI GGNCC 7 cut(s) 841, 850, 914, 1527, 1871, 1928, 2744
PspPPI RGGWCCY 1 cut(s) 2744
PspXI VCTCGAGB 1 cut(s) 498
PsrI GAACNNNNNNTAC 2 cut(s) 2720, 2752
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 4 cut(s) 1247, 1324, 2704, 3087
PsyI GACNNNGTC 1 cut(s) 283
PvuI CGATCG 1 cut(s) 1881
PvuII CAGCTG 5 cut(s) 32, 712, 2357, 2801, 3052
RsaI GTAC 8 cut(s) 306, 823, 1376, 1885, 2156, 2367, 2729, 2962
RsaNI GTAC 8 cut(s) 305, 822, 1375, 1884, 2155, 2366, 2728, 2961
RseI CAYNNNNRTG 3 cut(s) 1923, 1959, 3098
SacII CCGCGG 1 cut(s) 796
SapI GCTCTTC 1 cut(s) 2007
SaqAI TTAA 4 cut(s) 200, 2517, 2616, 2672
Sau96I GGNCC 7 cut(s) 841, 850, 914, 1527, 1871, 1928, 2744
ScrFI CCNGG 7 cut(s) 126, 313, 885, 1092, 1646, 1869, 2709
SduI GDGCHC 1 cut(s) 436
SexAI ACCWGGT 1 cut(s) 1090
SfaNI GCATC 8 cut(s) 4, 74, 431, 447, 1118, 2573, 2611, 2893
SfcI CTRYAG 1 cut(s) 2721
Sfr274I CTCGAG 1 cut(s) 498
Sfr303I CCGCGG 1 cut(s) 796
SfuI TTCGAA 1 cut(s) 251
SgrBI CCGCGG 1 cut(s) 796
SinI GGWCC 3 cut(s) 850, 1871, 2744
SlaI CTCGAG 1 cut(s) 498
SmiMI CAYNNNNRTG 3 cut(s) 1923, 1959, 3098
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
SspI AATATT 2 cut(s) 1234, 2540
SspMI CTAG 9 cut(s) 137, 194, 618, 669, 1215, 1382, 1391, 1898, 2568
StyD4I CCNGG 7 cut(s) 124, 311, 883, 1090, 1644, 1867, 2707
StyI CCWWGG 4 cut(s) 627, 2226, 2524, 2658
TaaI ACNGT 6 cut(s) 491, 644, 1026, 2166, 2732, 2785
TaiI ACGT 4 cut(s) 1366, 2268, 2668, 2962
TaqII GACCGA 1 cut(s) 1929
TatI WGTACW 2 cut(s) 1374, 2365
TauI GCSGC 5 cut(s) 580, 795, 1513, 1516, 1711
Tru1I TTAA 4 cut(s) 200, 2517, 2616, 2672
Tru9I TTAA 4 cut(s) 200, 2517, 2616, 2672
TscAI CASTG 4 cut(s) 496, 851, 2327, 2952
TseFI GTSAC 3 cut(s) 1966, 2247, 2288
TseI GCWGC 9 cut(s) 32, 257, 623, 761, 2018, 2231, 2639, 2921, 2936
Tsp45I GTSAC 3 cut(s) 1966, 2247, 2288
TspRI CASTG 4 cut(s) 496, 851, 2327, 2952
Tth111I GACNNNGTC 1 cut(s) 283
Van91I CCANNNNNTGG 2 cut(s) 1473, 1700
VpaK11BI GGWCC 3 cut(s) 850, 1871, 2744
XapI RAATTY 5 cut(s) 507, 907, 2304, 2333, 2507
XceI RCATGY 1 cut(s) 2314
XhoI CTCGAG 1 cut(s) 498
XmiI GTMKAC 2 cut(s) 2115, 2717
XmnI GAANNNNTTC 2 cut(s) 943, 2346
XspI CTAG 9 cut(s) 137, 194, 618, 669, 1215, 1382, 1391, 1898, 2568
Zsp2I ATGCAT 1 cut(s) 2605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.