MD12G1247800.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000071
Physical Location & Seq
Reverse (-)
31802118 .. 31805473
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1247800.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGCTCAATATAATCATAGTGTTGTTATGCATCTTGGTGGAACAAATAAATGGATAATTGATACTGAGGCCACTGATCATGTGACTAGTGACCCTAGAGTGTTTGATGAGTTATGTGACTATGTTCGTGATCCGTATATTACTAGTGCAAATGGAGCACTTTCCCCTGTGAAGGGTGAAGACACTATCTCTCTGACTCCAACCTTATCACTGGTTCGTGCTTTACTTGTTCCTGATGTTAAGTACAATCTTTTGTCGAGTCATTGTGCTTCATTTCATGTAAGTGATTCTACAGCTACTTTGTCATTTGATTTGATATATTCTGATGTGTGGGGTCCTGCGAAAGTTACTTCTAATGGATTCTGTTGGTTTGTTACTTTTATTGATGATTGTACTCGGGTAACTTGGGTGTTCTTGATGAAAAATAAGAGTGATGTTCCGTTACTTCTTCAAGAATTTTGTACAATGGTGTCTACTCAGTTTCAGACCAAAGTTAAGGTCTTTAGGTCTGATAACGGAGGAGAATATGTGAATCACATTTTGGCATGTTTTTTCCGTGATCATGGTATGATTCACCAGACAACTACTCCGTTTACACCCCAACAAAATGGTGTGTCCGAACGGAAGAATCGTCAAATAATGGAGGTTGCTCGCTCCTTGATGTTGGATAAATGTGTTCCTAATCATTTGTGGGGTCATGTTGTTTTGGCTGCTGTGTATTTGATCAATCGTGTTCCAAGTAGGGTTCTTGATTTTCAGACACCGTTTGATGTGCTACAAAAACATGTTTCTCTTGTTTCTGTCTCAAAGCTTCCTCCGAAAGTGTTTTGGTGTATTGTGTATGTGCATGTCTACTCTCATCAGCGGAGTAAACTTGATGCATGTGCTCTCCGGTGTGTCTTTATTGGGTATGCTAACAATCAAAAAGGCTATAAGTGTTATCATCCTCCGACTCAAAAAACTTATATTACCATGGATGTCACCTTCCAAGAGGAAGTTTCGTATTTTGTAAAGCCCTCTTCCGACTTTCCACTTCAGGGGGAGAGGAGGAGTGAAGTGCAGATTCGAAGAGATGATATGGATGATGTGTTACAGACAGAGTTACGAAAAGCCTTGTATGGTTTGAAACAATCTCCTAGAGCATGGTTTGGGAGGTTTACAAGTTTAATGAAGAAGTTTGGGTATATCCAAAGTCATTCAGATCATACTTTGTTTCTAAAGCGACAAAATGGTAAGCTAACTGCATTGATTATTTATGTTGATGACATGATAGTGACTGGTGATGATCAGAAAGAGATACAGCACCTTCAAAAGTACCTAGCTACTGAGTTTGAGATGAAAGAATTGGGTGAATTGAAGTATTTTCTTGGAAGGAAGTATGTTCTTGATTTGTTAGCTGAAACATGTATGTTAGATTGCAAACCTGTTGATACTCCGATTGAGCAGAATCATCGTCTGGGCTTATTTCCAGATCAAGTTCCTACTCATAAGGAACGGTATCAAAGGCTTGTGGTGAGATTAATTTATTTGTCTCACACTCGCCCTGACATTGCTTATGCAGTTAGTGTGGTAAGTCAGTTTATGCACTCACCTAGTGAAGCTCATATGGATGCAGTAACCCGTATTTTGAGGTACTTGAAGATGGCTCCTGGCAGATGCCTGGTTTTCTCCAAGAATGATCATTTGAATGTCGAAGGGTATATAGATGCAAATTGGGCAGATTCTATCACTGATCGGCGATCTACATCTGGATACTTTATGTTTGTGGGTGGTAATTTAGTTACTTGGATAAGCAAGAAACAAAAAGTGGTGGCTAAGTCAAGTGCAGAAGCTGAGTTTCGTGGTATGTCTCATGGTGTATGTGAGTTGTTGTGGTTGGAAAAATTGTTGAGAGATCTTGAGTTTAAACACAAAGGTGCTATGAAACTTCATTATGATAACAAGGCTGCTATTGAGATTGCTCATAATCCAGTGCAACATGATCGAACAAAACATGTGGAGATTGATCGACATTTCATTAAGGAAAAATTGGATGCTGGAATTATTATGTTTCCGTTTGTGAGATCTGAAGATCAACTTGCTGATGCTCTTACTAAGGCTGTGTCTAATAGTGCGTTTTCCGACTCGCTTGACAAGTTGGGCATGCGTGACATCTTTGCACCAACTTGA

Protein Analysis

723

Amino Acids

82.75

Weight (kDa)

8.05

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 18 - 88 7.4e-07 Pol polyprotein, beta-barrel domain
rve PF00665 105 - 200 1.1e-10 Integrase core domain
SH3_retrovirus PF25597 278 - 338 7.5e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 366 - 459 2.2e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 473, 852
AciI CCGC 1 cut(s) 865
AclWI GGATC 1 cut(s) 125
AcsI RAATTY 1 cut(s) 455
AcuI CTGAAG 2 cut(s) 1019, 2088
AdeI CACNNNGTG 1 cut(s) 1595
AfaI GTAC 5 cut(s) 245, 394, 463, 1316, 1634
AfiI CCNNNNNNNGG 3 cut(s) 172, 173, 1037
AflIII ACRYGT 3 cut(s) 784, 1403, 1993
AgsI TTSAA 6 cut(s) 452, 1126, 1310, 1357, 1639, 1687
AhlI ACTAGT 2 cut(s) 86, 143
AjnI CCWGG 2 cut(s) 1648, 1659
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AleI CACNNNNGTG 1 cut(s) 1914
AluBI AGCT 7 cut(s) 296, 811, 1237, 1322, 1397, 1601, 1832
AluI AGCT 7 cut(s) 296, 811, 1237, 1322, 1397, 1601, 1832
Alw21I GWGCWC 2 cut(s) 160, 889
Alw26I GTCTC 3 cut(s) 808, 1536, 1854
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 1832
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 2 cut(s) 710, 1946
ApoI RAATTY 1 cut(s) 455
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
AseI ATTAAT 1 cut(s) 1520
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 7 cut(s) 188, 566, 973, 1292, 1361, 1525, 1581
AsuII TTCGAA 1 cut(s) 1066
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 160, 889
BbvI GCAGC 2 cut(s) 697, 1933
BccI CCATC 1 cut(s) 1636
BcgI CGANNNNNNTGC 4 cut(s) 1433, 1467, 1964, 1998
BciT130I CCWGG 2 cut(s) 1650, 1661
BciVI GTATCC 1 cut(s) 1745
BclI TGATCA 5 cut(s) 76, 559, 723, 1285, 1678
BcoDI GTCTC 3 cut(s) 808, 1536, 1854
BcuI ACTAGT 2 cut(s) 86, 143
BfaI CTAG 6 cut(s) 87, 96, 144, 1137, 1319, 1593
BfmI CTRYAG 1 cut(s) 291
BfuI GTATCC 1 cut(s) 1745
BglII AGATCT 2 cut(s) 1894, 2063
BisI GCNGC 2 cut(s) 711, 1947
BlsI GCNGC 2 cut(s) 712, 1948
Bme1390I CCNGG 2 cut(s) 1650, 1661
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 336, 1647
BmrFI CCNGG 2 cut(s) 1650, 1661
BmsI GCATC 7 cut(s) 40, 868, 1600, 1646, 1696, 2023, 2074
BpiI GAAGAC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 1066
BpuEI CTTGAG 1 cut(s) 1919
BsaBI GATNNNNATC 1 cut(s) 1744
BsaJI CCNNGG 1 cut(s) 972
BsaWI WCCGGW 1 cut(s) 891
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 173, 1037
Bse1I ACTGG 3 cut(s) 216, 1282, 1970
Bse3DI GCAATG 1 cut(s) 1548
Bse8I GATNNNNATC 1 cut(s) 1744
BseBI CCWGG 2 cut(s) 1650, 1661
BseDI CCNNGG 1 cut(s) 972
BseGI GGATG 5 cut(s) 943, 982, 1087, 1615, 2038
BseJI GATNNNNATC 1 cut(s) 1744
BseLI CCNNNNNNNGG 3 cut(s) 172, 173, 1037
BseMI GCAATG 1 cut(s) 1548
BseMII CTCAG 4 cut(s) 57, 491, 1317, 1824
BseNI ACTGG 3 cut(s) 216, 1282, 1970
BseRI GAGGAG 3 cut(s) 534, 1060, 1063
BseXI GCAGC 2 cut(s) 697, 1933
BsgI GTGCAG 2 cut(s) 1079, 1845
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 160, 889
BsiHKCI CYCGRG 1 cut(s) 396
BsiSI CCGG 1 cut(s) 892
BslI CCNNNNNNNGG 3 cut(s) 172, 173, 1037
BsmAI GTCTC 3 cut(s) 808, 1536, 1854
BsnI GGCC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 396
Bsp119I TTCGAA 1 cut(s) 1066
Bsp1286I GDGCHC 2 cut(s) 160, 889
Bsp1407I TGTACA 1 cut(s) 461
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 1 cut(s) 865
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 4 cut(s) 58, 490, 1318, 1825
BspLI GGNNCC 2 cut(s) 336, 1647
BspPI GGATC 1 cut(s) 125
BspT104I TTCGAA 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 1548
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 3 cut(s) 216, 1282, 1970
BssECI CCNNGG 1 cut(s) 972
BssT1I CCWWGG 1 cut(s) 972
Bst2UI CCWGG 2 cut(s) 1650, 1661
Bst4CI ACNGT 2 cut(s) 765, 1497
Bst6I CTCTTC 2 cut(s) 1024, 1063
BstAUI TGTACA 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 1066
BstC8I GCNNGC 2 cut(s) 652, 2144
BstDEI CTNAG 6 cut(s) 66, 477, 1326, 1815, 1833, 2094
BstDSI CCRYGG 1 cut(s) 972
BstF5I GGATG 5 cut(s) 943, 982, 1087, 1615, 2038
BstMAI GTCTC 3 cut(s) 808, 1536, 1854
BstMWI GCNNNNNNNGC 2 cut(s) 155, 1715
BstNI CCWGG 2 cut(s) 1650, 1661
BstNSI RCATGY 7 cut(s) 549, 788, 851, 885, 1407, 1997, 2146
BstSCI CCNGG 2 cut(s) 1648, 1659
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 697, 1933
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 1894, 2063
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 2 cut(s) 1894, 2063
BsuI GTATCC 1 cut(s) 1745
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 972
BtsCI GGATG 5 cut(s) 943, 982, 1087, 1615, 2038
BtsIMutI CAGTG 4 cut(s) 72, 209, 1728, 1977
Cac8I GCNNGC 2 cut(s) 652, 2144
CaiI CAGNNNCTG 1 cut(s) 1832
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 5 cut(s) 244, 393, 462, 1315, 1633
CspCI CAANNNNNGTGG 2 cut(s) 1977, 2012
CviQI GTAC 5 cut(s) 244, 393, 462, 1315, 1633
DdeI CTNAG 6 cut(s) 66, 477, 1326, 1815, 1833, 2094
DraI TTTAAA 1 cut(s) 1906
DraIII CACNNNGTG 1 cut(s) 1595
Eam1104I CTCTTC 2 cut(s) 1024, 1063
EarI CTCTTC 2 cut(s) 1024, 1063
Eco130I CCWWGG 1 cut(s) 972
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 1019, 2088
Eco88I CYCGRG 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 1648, 1659
EcoT14I CCWWGG 1 cut(s) 972
EcoT22I ATGCAT 2 cut(s) 33, 883
ErhI CCWWGG 1 cut(s) 972
FalI AAGNNNNNCTT 2 cut(s) 2061, 2093
FauNDI CATATG 1 cut(s) 1605
FbaI TGATCA 5 cut(s) 76, 559, 723, 1285, 1678
FblI GTMKAC 2 cut(s) 473, 852
Fnu4HI GCNGC 2 cut(s) 711, 1947
FokI GGATG 5 cut(s) 930, 989, 1094, 1622, 2045
Fsp4HI GCNGC 2 cut(s) 711, 1947
FspBI CTAG 6 cut(s) 87, 96, 144, 1137, 1319, 1593
GluI GCNGC 2 cut(s) 711, 1947
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 809
HpaII CCGG 1 cut(s) 892
HphI GGTGA 7 cut(s) 188, 566, 973, 1292, 1361, 1525, 1581
Hpy166II GTNNAC 5 cut(s) 474, 594, 853, 872, 1158
Hpy188III TCNNGA 9 cut(s) 128, 233, 415, 452, 749, 1385, 1469, 1749, 1898
Hpy8I GTNNAC 5 cut(s) 474, 594, 853, 872, 1158
HpyAV CCTTC 5 cut(s) 166, 994, 1316, 1365, 1688
HpyCH4III ACNGT 2 cut(s) 765, 1497
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 1715
HpyF3I CTNAG 6 cut(s) 66, 477, 1326, 1815, 1833, 2094
Ksp22I TGATCA 5 cut(s) 76, 559, 723, 1285, 1678
LmnI GCTCC 3 cut(s) 155, 659, 1651
Lsp1109I GCAGC 2 cut(s) 697, 1933
LweI GCATC 7 cut(s) 40, 868, 1600, 1646, 1696, 2023, 2074
MaeI CTAG 6 cut(s) 87, 96, 144, 1137, 1319, 1593
MboII GAAGA 8 cut(s) 191, 440, 637, 1011, 1080, 1183, 1651, 2081
MflI RGATCY 2 cut(s) 1894, 2063
MhlI GDGCHC 2 cut(s) 160, 889
MlyI GAGTC 4 cut(s) 190, 268, 946, 2117
MmeI TCCRAC 6 cut(s) 224, 645, 974, 1047, 1857, 2145
Mph1103I ATGCAT 2 cut(s) 33, 883
MseI TTAA 6 cut(s) 240, 495, 1166, 1520, 1905, 2019
MslI CAYNNNNRTG 6 cut(s) 36, 282, 1271, 1608, 1686, 1914
MspA1I CMGCKG 1 cut(s) 865
MspI CCGG 1 cut(s) 892
MspR9I CCNGG 2 cut(s) 1650, 1661
MssI GTTTAAAC 1 cut(s) 1906
MvaI CCWGG 2 cut(s) 1650, 1661
MwoI GCNNNNNNNGC 2 cut(s) 155, 1715
NcoI CCATGG 1 cut(s) 972
NdeI CATATG 1 cut(s) 1605
NlaIV GGNNCC 2 cut(s) 336, 1647
NmuCI GTSAC 6 cut(s) 82, 89, 116, 979, 1273, 2147
NsiI ATGCAT 2 cut(s) 33, 883
NspI RCATGY 7 cut(s) 549, 788, 851, 885, 1407, 1997, 2146
NspV TTCGAA 1 cut(s) 1066
OliI CACNNNNGTG 1 cut(s) 1914
PaeI GCATGC 1 cut(s) 2146
PciI ACATGT 3 cut(s) 784, 1403, 1993
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PfeI GAWTC 8 cut(s) 287, 360, 532, 571, 628, 1063, 1447, 1721
PkrI GCNGC 2 cut(s) 712, 1948
PleI GAGTC 4 cut(s) 190, 267, 946, 2117
PmeI GTTTAAAC 1 cut(s) 1906
PpsI GAGTC 4 cut(s) 190, 267, 946, 2117
PpuMI RGGWCCY 1 cut(s) 335
PscI ACATGT 3 cut(s) 784, 1403, 1993
PshBI ATTAAT 1 cut(s) 1520
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 1648, 1659
PspGI CCWGG 2 cut(s) 1648, 1659
PspN4I GGNNCC 2 cut(s) 336, 1647
PspPI GGNCC 1 cut(s) 335
PspPPI RGGWCCY 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 1832
PsuI RGATCY 2 cut(s) 1894, 2063
RsaI GTAC 5 cut(s) 245, 394, 463, 1316, 1634
RsaNI GTAC 5 cut(s) 244, 393, 462, 1315, 1633
RseI CAYNNNNRTG 6 cut(s) 36, 282, 1271, 1608, 1686, 1914
SaqAI TTAA 6 cut(s) 240, 495, 1166, 1520, 1905, 2019
SatI GCNGC 2 cut(s) 711, 1947
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 4 cut(s) 190, 268, 946, 2117
ScrFI CCNGG 2 cut(s) 1650, 1661
SduI GDGCHC 2 cut(s) 160, 889
SfaNI GCATC 7 cut(s) 40, 868, 1600, 1646, 1696, 2023, 2074
SfcI CTRYAG 1 cut(s) 291
SfuI TTCGAA 1 cut(s) 1066
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 6 cut(s) 36, 282, 1271, 1608, 1686, 1914
SmlI CTYRAG 1 cut(s) 1898
SmoI CTYRAG 1 cut(s) 1898
SpeI ACTAGT 2 cut(s) 86, 143
SphI GCATGC 1 cut(s) 2146
SsiI CCGC 1 cut(s) 865
SspMI CTAG 6 cut(s) 87, 96, 144, 1137, 1319, 1593
StyD4I CCNGG 2 cut(s) 1648, 1659
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 2 cut(s) 765, 1497
TaqI TCGA 5 cut(s) 257, 1066, 1692, 1984, 2008
TatI WGTACW 3 cut(s) 243, 392, 461
TfiI GAWTC 8 cut(s) 287, 360, 532, 571, 628, 1063, 1447, 1721
Tru1I TTAA 6 cut(s) 240, 495, 1166, 1520, 1905, 2019
Tru9I TTAA 6 cut(s) 240, 495, 1166, 1520, 1905, 2019
TscAI CASTG 4 cut(s) 79, 216, 1735, 1977
TseFI GTSAC 6 cut(s) 82, 89, 116, 979, 1273, 2147
TseI GCWGC 2 cut(s) 710, 1946
Tsp45I GTSAC 6 cut(s) 82, 89, 116, 979, 1273, 2147
TspDTI ATGAA 8 cut(s) 261, 266, 434, 1184, 1352, 1919, 1937, 2005
TspGWI ACGGA 7 cut(s) 123, 429, 531, 545, 579, 637, 2043
TspRI CASTG 4 cut(s) 79, 216, 1735, 1977
VpaK11BI GGWCC 1 cut(s) 335
VspI ATTAAT 1 cut(s) 1520
XapI RAATTY 1 cut(s) 455
XceI RCATGY 7 cut(s) 549, 788, 851, 885, 1407, 1997, 2146
XmiI GTMKAC 2 cut(s) 473, 852
XspI CTAG 6 cut(s) 87, 96, 144, 1137, 1319, 1593
Zsp2I ATGCAT 2 cut(s) 33, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.