RchiOBHm_Chr2g0154181

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Forward (+)
71376675 .. 71380351
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52323

Sequence Viewer

Length: 3045 bp
ATGGAGATGACCACTGCTGGCAATCGTCGCATAAGTGAGGTGCATCATATTCAGAAAAAACCTTCCACCGATGAAGAACAGATCTGGTTATGGCATAAAAGATTGGGACATCCATCATTTGGGTATCTCCAAACTCTCTTCCCCTTACTGTTTCAGCATAATAGACCATCAGACTTTAAATGCAACACTTGCATTTTAGGCAAGAGTCATCGTGTAACCTTTCCATTGAGCTCGAATAAAAGTATTTCCCCTTTTGATCTTGTTCACTCTGATGTCTGGGGACCTTGTCCGGCAACGCCCTCTGGAATGAAATGGTTTGTTTTATTTGTCGATGATTGCACCCGGATGAGTTGGATCTACTTGCTGCGAAATAAAAGTGATGTTTTCCCGGTTTTTCAGACTTTTCATGCCATGATCCAAACTCAATTCCAAACACCTATTAAAGTCCTTCGTTCCGATAATGGTGGAGAATATGTGAACCATCCCTTTCACCAGTATCTAGCTACCCATGGCATCATTCACCAGACCACATGTCCTCAAACTCCTCAACAAAATGGTGTGGCAGAAAGAAAGAACCGCCACATTCTAGAAGTTGCTCGCTCCATGTTAATCGGAGCTCATATGCCAGAATCTTTTTGGGGTGATGCCATCCTAACTACTGCTTACCTCATAAATAGACTCCCTACACAAGCCCTTCAGTCTACAGCTAAGGAAACCAGTCCACCACCACAAACACCAATTCAAGCTCTTGCCAGTCATTTACCTTTACCACCTACCCATGCCCTTGATCCAAAGATTTTTGGTTGTGTAGCTTATGTACATATCCATCGAAACCAGAGGACTAAATTGGATGCTTGTGCTGAAAAGTGTATCTTTATTGGCTATGCTTCCACTCAAAAGGGATATCGGTGCTATAGTCCGACCACTAAGCGTACCTATGTTTCCATGGATGTTACATTCCATGAACATGATCTACATTTTTCCCCGAAGCCTACTATTCAGGGGGAGAATGATATAGAGGAAGAGCAGTCTTCGGGGGATTGCTTGTGTCCACCCTTGGAACCCATGGACATCCAAGAGCATGGAAATACTGGAGAAACAAGAATTGAGCATGGAAATACTGAAAGGAGCAACCAGTTACCGACATCAGAAATGGCAGCTGAACATGGAAATCAGCTACCAACTCCAGAATCAGCAGTGAGAGATATTTCTTCCCCTTCTACTACCCCATCAACACCAATGCTCCCTGAGGATGATCCAGAGGTAATTAATAACCCTGTGTTTGAATGCCATGATAATGTAAATTCTATGCCTAATACTGAAATAGAATCAAGTGACATCATAGGGGTTCATAAATTGCCATTTCGGAAAAATAGAGGTCAACCAAAGGTTCAATATGAACCAGATCCTAAAGCCAAGGCCAAATATCCCATAAGCAATTACATGTCATCCCATAGGTTATCCAAGTCATATGCGTCCTTTGTATTTCATTTATCTGCTATTATCATTCCAAATAAAGTGCAGGATGCTCTAGCAAATCCTAAGTGGACTAAAGCTATGGAAGATGAAATGGAAGCATTGCAAAAGAATCACACATGGGAACTAGTGCCAAAACCCGCAGGAAAGAGAGCTGTAGGATGCAGATGGATCTATACATTGAAGTACGCAGCTGATGGTACACTGGATAGATACAAAGCCAGGTTGGTTGCAAAAGGGTATACTCAGACATATGGTATTGACTATCAAGAAACTTTTGCACCAGTGGCCAAGATGAATACCGTTCGAGTACTACTGTCATTAGCGGCTAACCTAGACTGGCCTCTGAAGCAGTTTGATGTAAAGAATGCCTTCCTACATGGAGACTTGAAGGAAGAAGTCTACATGGATCTACCTCCTGGCTATTCTCTACCTTCACAAGCAGGAATGGTGTGCAAGTTGAAAAAATCCCTTTATGGACTAAAACAGTCCCCCAGAGCATGGTTTGGAAGATTCAGCCAATCTATGAAACAGTTTGGTTACAAACAAAGCAACTCTGACCATACACTGTTTCTGAAGCACAGAAAAGATAAACTTACTGCACTTATCATTTATGTTGATGATATGATTGTGACAGGTAATGACCCGGAAGAAGTCAAGAAACTAGAAGCACATCTATCATCTGTTTTTGAGATGAAAGATCTTGGAGGACTGAAATACTTCTTGGGAATTGAGGTGGCTAGATCAAAAGAAGGCATTGCCCTATCTCAAAGGAAGTATGTGGTTGACTTACTGATAGAGACCGGTTTACTAGCATGTAGACCAGCAGATACACCGATGGAGCAAAATCACAAATTAGCAGAATATCCAGACCAAGTGCCTACTAACAAAGAGAGATATCAAAGGTTAGTAGGCAAGCTCATTTATCTCTCACACACCCGACCTGATATTGCATATGCTGTAAGTGTTGTAAGCCAATTCATGCATGCACCAAGTGAAGATCACATGGAAGCAGTACATAGAATTCTGAGGTATTTGAAGACAAATCCCGGTAAAGGGATCATGTTTTCCAAGAATAACCACACAGACATAGCTGGATACACAGATGCAGATTGGGCTGGTTCTATCACTGATAGAAGGTCTACTTCAGGTTACTTTACTTTTGTGGGAGGTAACTTGGTCACGTGGAGAAGCAAGAAGCAAAAAGTGGTTGCTAGATCCAGTGCAGAAGCTGAGTACAGAGGAATGGCTCATGGAGTATGCGAAATGCTATGGCTTCGTCATTTGATGAAAGACTTGGGATTCAAACCCAAGTATGCTATGAATTTGTATTGTGACAATAAAGCAGCCATTGATATAGCACACAATCCAGTTCAACATGATAGAACAAAACATGTTGAAGTGGATCGACATTTCATCAAGGAGAAGTTGGAGTCAGAAACCATTAACATGCCACATGTGCGTACTGAAGATCAACTTGCAGATATCCTGACCAAAGCTGTTTCTGGAAAAGTGTTCCACAGCTCACTAGACAAGTTGGGTATGCGAGACATCTATTCCCCAACTTGA

Protein Analysis

1014

Amino Acids

115.06

Weight (kDa)

8.48

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 22 - 69 7e-14 GAG-pre-integrase domain
rve PF00665 84 - 182 1e-12 Integrase core domain
SH3_retrovirus PF25597 269 - 326 7.2e-25 Retroviral polymerase SH3-like domain
RVT_2 PF07727 530 - 773 1.6e-71 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 119, 1977
AccI GTMKAC 5 cut(s) 701, 1718, 1878, 2296, 2618
AciI CCGC 3 cut(s) 577, 1617, 1802
AcoI YGGCCR 1 cut(s) 1764
AcsI RAATTY 3 cut(s) 1303, 2499, 2800
AcuI CTGAAG 5 cut(s) 680, 1844, 2072, 2607, 2964
AcvI CACGTG 1 cut(s) 2661
AdeI CACNNNGTG 1 cut(s) 2471
AfaI GTAC 8 cut(s) 819, 934, 1664, 1678, 1788, 2493, 2714, 2941
AfiI CCNNNNNNNGG 4 cut(s) 119, 1977, 2531, 2532
AflIII ACRYGT 4 cut(s) 530, 1443, 2869, 2932
AgeI ACCGGT 1 cut(s) 2279
AhdI GACNNNNNGTC 1 cut(s) 531
AhlI ACTAGT 1 cut(s) 1603
AjnI CCWGG 2 cut(s) 1697, 1894
AjuI GAANNNNNNNTTGG 2 cut(s) 2183, 2215
Alw21I GWGCWC 2 cut(s) 233, 619
Alw26I GTCTC 3 cut(s) 1854, 2270, 3018
AlwNI CAGNNNCTG 1 cut(s) 2708
AoxI GGCC 3 cut(s) 1419, 1764, 1817
ApeKI GCWGC 4 cut(s) 364, 1157, 1667, 2822
ApoI RAATTY 3 cut(s) 1303, 2499, 2800
AseI ATTAAT 1 cut(s) 1269
AsiGI ACCGGT 1 cut(s) 2279
Asp700I GAANNNNTTC 2 cut(s) 1847, 2195
AspS9I GGNCC 1 cut(s) 281
AsuC2I CCSGG 4 cut(s) 343, 389, 2123, 2526
AsuHPI GGTGA 3 cut(s) 482, 512, 653
AvaII GGWCC 1 cut(s) 281
AxyI CCTNAGG 1 cut(s) 1248
BalI TGGCCA 1 cut(s) 1766
BanII GRGCYC 2 cut(s) 233, 619
BbrPI CACGTG 1 cut(s) 2661
BbsI GAAGAC 2 cut(s) 1023, 2522
Bbv12I GWGCWC 2 cut(s) 233, 619
BbvI GCAGC 4 cut(s) 351, 1169, 1679, 2834
BccI CCATC 9 cut(s) 121, 175, 489, 656, 834, 1237, 1638, 1667, 2308
BciT130I CCWGG 2 cut(s) 1699, 1896
BciVI GTATCC 1 cut(s) 2567
BcnI CCSGG 4 cut(s) 343, 389, 2123, 2526
BcoDI GTCTC 3 cut(s) 1854, 2270, 3018
BcuI ACTAGT 1 cut(s) 1603
BfmI CTRYAG 3 cut(s) 702, 913, 1632
BfuI GTATCC 1 cut(s) 2567
BglII AGATCT 2 cut(s) 81, 2176
BisI GCNGC 5 cut(s) 365, 1158, 1668, 1803, 2823
BlsI GCNGC 5 cut(s) 366, 1159, 1669, 1804, 2824
BmcAI AGTACT 1 cut(s) 1788
Bme1390I CCNGG 6 cut(s) 343, 389, 1699, 1896, 2123, 2526
Bme18I GGWCC 1 cut(s) 281
BmeRI GACNNNNNGTC 1 cut(s) 531
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 282, 1062
BmrFI CCNGG 6 cut(s) 343, 389, 1699, 1896, 2123, 2526
BmsI GCATC 7 cut(s) 52, 522, 634, 841, 1516, 1628, 2572
BpiI GAAGAC 2 cut(s) 1023, 2522
BpmI CTGGAG 2 cut(s) 1113, 1170
Bpu10I CCTNAGC 1 cut(s) 708
BpuMI CCSGG 4 cut(s) 343, 389, 2123, 2526
BsaAI YACGTR 1 cut(s) 2661
BsaI GGTCTC 1 cut(s) 2270
BsaJI CCNNGG 5 cut(s) 508, 945, 1056, 1065, 1416
BsaWI WCCGGW 1 cut(s) 2279
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 1227, 1257
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 1977, 2531, 2532
Bse118I RCCGGY 1 cut(s) 2279
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 2 cut(s) 1577, 2232
BseBI CCWGG 2 cut(s) 1699, 1896
BseDI CCNNGG 5 cut(s) 508, 945, 1056, 1065, 1416
BseLI CCNNNNNNNGG 4 cut(s) 119, 1977, 2531, 2532
BseMI GCAATG 2 cut(s) 1577, 2232
BseMII CTCAG 4 cut(s) 1239, 1736, 2495, 2700
BseRI GAGGAG 1 cut(s) 534
BseXI GCAGC 4 cut(s) 351, 1169, 1679, 2834
BsgI GTGCAG 3 cut(s) 1541, 2061, 2721
BshFI GGCC 3 cut(s) 1421, 1766, 1819
BshTI ACCGGT 1 cut(s) 2279
BsiHKAI GWGCWC 2 cut(s) 233, 619
BsiSI CCGG 6 cut(s) 290, 343, 389, 2123, 2280, 2526
BslFI GGGAC 3 cut(s) 120, 294, 1951
BslI CCNNNNNNNGG 4 cut(s) 119, 1977, 2531, 2532
BsmAI GTCTC 3 cut(s) 1854, 2270, 3018
BsmFI GGGAC 3 cut(s) 120, 294, 1951
BsmI GAATGC 2 cut(s) 1292, 1849
BsnI GGCC 3 cut(s) 1421, 1766, 1819
Bso31I GGTCTC 1 cut(s) 2270
Bsp1286I GDGCHC 2 cut(s) 233, 619
Bsp1407I TGTACA 1 cut(s) 817
Bsp19I CCATGG 3 cut(s) 508, 945, 1065
BspACI CCGC 3 cut(s) 577, 1617, 1802
BspANI GGCC 3 cut(s) 1421, 1766, 1819
BspCNI CTCAG 4 cut(s) 1240, 1735, 2496, 2701
BspLI GGNNCC 2 cut(s) 282, 1062
BspQI GCTCTTC 1 cut(s) 1017
BspTNI GGTCTC 1 cut(s) 2270
BsrDI GCAATG 2 cut(s) 1577, 2232
BsrFI RCCGGY 1 cut(s) 2279
BsrGI TGTACA 1 cut(s) 817
BssAI RCCGGY 1 cut(s) 2279
BssECI CCNNGG 5 cut(s) 508, 945, 1056, 1065, 1416
BssNAI GTATAC 1 cut(s) 1719
BssT1I CCWWGG 5 cut(s) 508, 945, 1056, 1065, 1416
Bst1107I GTATAC 1 cut(s) 1719
Bst2UI CCWGG 2 cut(s) 1699, 1896
Bst4CI ACNGT 6 cut(s) 150, 1780, 1794, 1965, 2010, 2046
Bst6I CTCTTC 2 cut(s) 143, 1017
BstAPI GCANNNNNTGC 1 cut(s) 189
BstAUI TGTACA 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 2661
BstC8I GCNNGC 4 cut(s) 19, 598, 2393, 2463
BstDEI CTNAG 7 cut(s) 708, 927, 1248, 1542, 1722, 2504, 2709
BstDSI CCRYGG 3 cut(s) 508, 945, 1065
BstMAI GTCTC 3 cut(s) 1854, 2270, 3018
BstMWI GCNNNNNNNGC 7 cut(s) 27, 189, 198, 1763, 1825, 2591, 2935
BstNI CCWGG 2 cut(s) 1699, 1896
BstNSI RCATGY 7 cut(s) 534, 1447, 2295, 2465, 2873, 2929, 2936
BstSCI CCNGG 6 cut(s) 341, 387, 1697, 1894, 2121, 2524
BstSFI CTRYAG 3 cut(s) 702, 913, 1632
BstV1I GCAGC 4 cut(s) 351, 1169, 1679, 2834
BstV2I GAAGAC 2 cut(s) 1023, 2522
BstX2I RGATCY 7 cut(s) 81, 354, 1405, 1647, 1885, 2176, 2693
BstXI CCANNNNNNTGG 1 cut(s) 1082
BstYI RGATCY 7 cut(s) 81, 354, 1405, 1647, 1885, 2176, 2693
BstZ17I GTATAC 1 cut(s) 1719
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 1 cut(s) 2567
BsuRI GGCC 3 cut(s) 1421, 1766, 1819
BtgI CCRYGG 3 cut(s) 508, 945, 1065
BtsI GCAGTG 2 cut(s) 12, 1203
BtsIMutI CAGTG 7 cut(s) 12, 1203, 1679, 1767, 2042, 2604, 2704
Cac8I GCNNGC 4 cut(s) 19, 598, 2393, 2463
CaiI CAGNNNCTG 1 cut(s) 2708
Cfr10I RCCGGY 1 cut(s) 2279
Cfr13I GGNCC 1 cut(s) 281
CseI GACGC 1 cut(s) 1464
Csp6I GTAC 8 cut(s) 818, 933, 1663, 1677, 1787, 2492, 2713, 2940
CspAI ACCGGT 1 cut(s) 2279
CviQI GTAC 8 cut(s) 818, 933, 1663, 1677, 1787, 2492, 2713, 2940
DdeI CTNAG 7 cut(s) 708, 927, 1248, 1542, 1722, 2504, 2709
DraI TTTAAA 1 cut(s) 178
DraIII CACNNNGTG 1 cut(s) 2471
DriI GACNNNNNGTC 1 cut(s) 531
EaeI YGGCCR 1 cut(s) 1764
Eam1104I CTCTTC 2 cut(s) 143, 1017
Eam1105I GACNNNNNGTC 1 cut(s) 531
EarI CTCTTC 2 cut(s) 143, 1017
Ecl136II GAGCTC 2 cut(s) 231, 617
Eco130I CCWWGG 5 cut(s) 508, 945, 1056, 1065, 1416
Eco24I GRGCYC 2 cut(s) 233, 619
Eco31I GGTCTC 1 cut(s) 2270
Eco32I GATATC 3 cut(s) 905, 2375, 2962
Eco47I GGWCC 1 cut(s) 281
Eco53kI GAGCTC 2 cut(s) 231, 617
Eco57I CTGAAG 5 cut(s) 680, 1844, 2072, 2607, 2964
Eco72I CACGTG 1 cut(s) 2661
Eco81I CCTNAGG 1 cut(s) 1248
EcoICRI GAGCTC 2 cut(s) 231, 617
EcoO109I RGGNCCY 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 2499
EcoRII CCWGG 2 cut(s) 1697, 1894
EcoRV GATATC 3 cut(s) 905, 2375, 2962
EcoT14I CCWWGG 5 cut(s) 508, 945, 1056, 1065, 1416
EcoT22I ATGCAT 1 cut(s) 2463
EcoT38I GRGCYC 2 cut(s) 233, 619
ErhI CCWWGG 5 cut(s) 508, 945, 1056, 1065, 1416
FaqI GGGAC 3 cut(s) 120, 294, 1951
FauI CCCGC 1 cut(s) 1624
FauNDI CATATG 4 cut(s) 621, 1471, 1729, 2431
FblI GTMKAC 5 cut(s) 701, 1718, 1878, 2296, 2618
Fnu4HI GCNGC 5 cut(s) 365, 1158, 1668, 1803, 2823
FriOI GRGCYC 2 cut(s) 233, 619
Fsp4HI GCNGC 5 cut(s) 365, 1158, 1668, 1803, 2823
GluI GCNGC 5 cut(s) 365, 1158, 1668, 1803, 2823
GsuI CTGGAG 2 cut(s) 1113, 1170
HaeIII GGCC 3 cut(s) 1421, 1766, 1819
HapII CCGG 6 cut(s) 290, 343, 389, 2123, 2280, 2526
HgaI GACGC 1 cut(s) 1464
HincII GTYRAC 2 cut(s) 1382, 2263
HindII GTYRAC 2 cut(s) 1382, 2263
HinfI GANTC 9 cut(s) 205, 629, 678, 1190, 1328, 1588, 1989, 2778, 2909
HpaII CCGG 6 cut(s) 290, 343, 389, 2123, 2280, 2526
HphI GGTGA 3 cut(s) 482, 512, 653
Hpy188III TCNNGA 9 cut(s) 303, 587, 1187, 1259, 1745, 2134, 2345, 2965, 2982
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 9 cut(s) 72, 458, 704, 1227, 1858, 1861, 1920, 2222, 2607
HpyCH4III ACNGT 6 cut(s) 150, 1780, 1794, 1965, 2010, 2046
HpyCH4IV ACGT 1 cut(s) 2660
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 189, 198, 1763, 1825, 2591, 2935
HpyF3I CTNAG 7 cut(s) 708, 927, 1248, 1542, 1722, 2504, 2709
HpySE526I ACGT 1 cut(s) 2660
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 5 cut(s) 605, 614, 1128, 1248, 2317
Lsp1109I GCAGC 4 cut(s) 351, 1169, 1679, 2834
LweI GCATC 7 cut(s) 52, 522, 634, 841, 1516, 1628, 2572
MaeII ACGT 1 cut(s) 2660
MflI RGATCY 7 cut(s) 81, 354, 1405, 1647, 1885, 2176, 2693
MhlI GDGCHC 2 cut(s) 233, 619
MlsI TGGCCA 1 cut(s) 1766
MluNI TGGCCA 1 cut(s) 1766
MlyI GAGTC 3 cut(s) 214, 672, 2918
MmeI TCCRAC 3 cut(s) 332, 944, 2886
Mox20I TGGCCA 1 cut(s) 1766
Mph1103I ATGCAT 1 cut(s) 2463
MroXI GAANNNNTTC 2 cut(s) 1847, 2195
MscI TGGCCA 1 cut(s) 1766
MseI TTAA 5 cut(s) 177, 441, 608, 1269, 2922
MslI CAYNNNNRTG 5 cut(s) 270, 344, 966, 1296, 2924
Msp20I TGGCCA 1 cut(s) 1766
MspA1I CMGCKG 2 cut(s) 1160, 1670
MspI CCGG 6 cut(s) 290, 343, 389, 2123, 2280, 2526
MspR9I CCNGG 6 cut(s) 343, 389, 1699, 1896, 2123, 2526
Mva1269I GAATGC 2 cut(s) 1292, 1849
MvaI CCWGG 2 cut(s) 1699, 1896
MwoI GCNNNNNNNGC 7 cut(s) 27, 189, 198, 1763, 1825, 2591, 2935
NciI CCSGG 4 cut(s) 343, 389, 2123, 2526
NcoI CCATGG 3 cut(s) 508, 945, 1065
NdeI CATATG 4 cut(s) 621, 1471, 1729, 2431
NlaIV GGNNCC 2 cut(s) 282, 1062
NmuCI GTSAC 4 cut(s) 1334, 2107, 2656, 2810
NsiI ATGCAT 1 cut(s) 2463
NspI RCATGY 7 cut(s) 534, 1447, 2295, 2465, 2873, 2929, 2936
PaeI GCATGC 1 cut(s) 2465
PciI ACATGT 4 cut(s) 530, 1443, 2869, 2932
PciSI GCTCTTC 1 cut(s) 1017
PctI GAATGC 2 cut(s) 1292, 1849
PdmI GAANNNNTTC 2 cut(s) 1847, 2195
PfeI GAWTC 6 cut(s) 629, 1190, 1328, 1588, 1989, 2778
PflFI GACNNNGTC 1 cut(s) 285
PflMI CCANNNNNTGG 2 cut(s) 119, 1977
PinAI ACCGGT 1 cut(s) 2279
PkrI GCNGC 5 cut(s) 366, 1159, 1669, 1804, 2824
PleI GAGTC 3 cut(s) 213, 672, 2917
PmaCI CACGTG 1 cut(s) 2661
PmlI CACGTG 1 cut(s) 2661
PpsI GAGTC 3 cut(s) 213, 672, 2917
Ppu21I YACGTR 1 cut(s) 2661
PpuMI RGGWCCY 1 cut(s) 281
PscI ACATGT 4 cut(s) 530, 1443, 2869, 2932
PshBI ATTAAT 1 cut(s) 1269
Psp124BI GAGCTC 2 cut(s) 233, 619
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 2 cut(s) 1697, 1894
PspCI CACGTG 1 cut(s) 2661
PspGI CCWGG 2 cut(s) 1697, 1894
PspN4I GGNNCC 2 cut(s) 282, 1062
PspPI GGNCC 1 cut(s) 281
PspPPI RGGWCCY 1 cut(s) 281
PsrI GAACNNNNNNTAC 2 cut(s) 957, 989
PstNI CAGNNNCTG 1 cut(s) 2708
PsuI RGATCY 7 cut(s) 81, 354, 1405, 1647, 1885, 2176, 2693
PsyI GACNNNGTC 1 cut(s) 285
PvuII CAGCTG 2 cut(s) 1160, 1670
RsaI GTAC 8 cut(s) 819, 934, 1664, 1678, 1788, 2493, 2714, 2941
RsaNI GTAC 8 cut(s) 818, 933, 1663, 1677, 1787, 2492, 2713, 2940
RseI CAYNNNNRTG 5 cut(s) 270, 344, 966, 1296, 2924
SacI GAGCTC 2 cut(s) 233, 619
SapI GCTCTTC 1 cut(s) 1017
SaqAI TTAA 5 cut(s) 177, 441, 608, 1269, 2922
SatI GCNGC 5 cut(s) 365, 1158, 1668, 1803, 2823
Sau96I GGNCC 1 cut(s) 281
ScaI AGTACT 1 cut(s) 1788
SchI GAGTC 3 cut(s) 214, 672, 2918
ScrFI CCNGG 6 cut(s) 343, 389, 1699, 1896, 2123, 2526
SduI GDGCHC 2 cut(s) 233, 619
SfaNI GCATC 7 cut(s) 52, 522, 634, 841, 1516, 1628, 2572
SfcI CTRYAG 3 cut(s) 702, 913, 1632
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 5 cut(s) 270, 344, 966, 1296, 2924
SpeI ACTAGT 1 cut(s) 1603
SphI GCATGC 1 cut(s) 2465
SsiI CCGC 3 cut(s) 577, 1617, 1802
SstI GAGCTC 2 cut(s) 233, 619
StyD4I CCNGG 6 cut(s) 341, 387, 1697, 1894, 2121, 2524
StyI CCWWGG 5 cut(s) 508, 945, 1056, 1065, 1416
TaaI ACNGT 6 cut(s) 150, 1780, 1794, 1965, 2010, 2046
TaiI ACGT 1 cut(s) 2663
TaqI TCGA 5 cut(s) 233, 330, 829, 1783, 2884
TatI WGTACW 4 cut(s) 817, 1786, 2491, 2712
TauI GCSGC 1 cut(s) 1805
TfiI GAWTC 6 cut(s) 629, 1190, 1328, 1588, 1989, 2778
Tru1I TTAA 5 cut(s) 177, 441, 608, 1269, 2922
Tru9I TTAA 5 cut(s) 177, 441, 608, 1269, 2922
TscAI CASTG 7 cut(s) 19, 1203, 1686, 1767, 2049, 2611, 2704
TseFI GTSAC 4 cut(s) 1334, 2107, 2656, 2810
TseI GCWGC 4 cut(s) 364, 1157, 1667, 2822
Tsp45I GTSAC 4 cut(s) 1334, 2107, 2656, 2810
TspRI CASTG 7 cut(s) 19, 1203, 1686, 1767, 2049, 2611, 2704
Tth111I GACNNNGTC 1 cut(s) 285
Van91I CCANNNNNTGG 2 cut(s) 119, 1977
VpaK11BI GGWCC 1 cut(s) 281
VspI ATTAAT 1 cut(s) 1269
XapI RAATTY 3 cut(s) 1303, 2499, 2800
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 7 cut(s) 534, 1447, 2295, 2465, 2873, 2929, 2936
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XmiI GTMKAC 5 cut(s) 701, 1718, 1878, 2296, 2618
XmnI GAANNNNTTC 2 cut(s) 1847, 2195
ZrmI AGTACT 1 cut(s) 1788
Zsp2I ATGCAT 1 cut(s) 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.