MD02G1250500.v1.1

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000071
Physical Location & Seq
Forward (+)
30224839 .. 30228450
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1250500.v1.1.491

Sequence Viewer

Length: 2940 bp
ATGGCTCCCTGTGCTTGTCCTTTTTCAAATGGATTTGACCGAACCGGACATGAGAGTAGGACCGATCGAGACCAGTTCAAGACTTGTGATCCCCACTGTAACAAGTCGTGTACCTCGATTGTCGAATCGAAAATAGATTCTAATATAGGTAACAAAGGTAAGGCTTTAAAAGTTTCTGCATCTGTTACGAATAATACATGGATAATTGATTCCGGTGCTACTGAACATATGACTTGTGAATCTAGGCAGGTACAAATCCTAAAACCACCCACCAAAACTGTTGTGAGTGTTGCCAACGGTAATGTTGTTCTCATTATTGGAGAAGGCACTGTCTCCCTGTCAAATACCCTTAGTCTTGATACCGTGCTTGTTGTTCCTTCTCTTGACCATAATTTATTATCTGTTGCTCAAATAACTGTCGCCCTGCATTGTCTTGTGATTTTTTGGCCTTCCTTTTGTGTTTTTAAGGACATTCGGACTCACAAGACGATTGGTTATGGTATTAGGAAGGGGAAGCTCTATTACTTGGAGCTGACATCAAACAGTTTTCGAATGTTGACTCACGCTCTTGCAGTGGACGGAAATTCGGGGGATAGGAATAAAGCTTCGGAGGTTTGGTTGTGGCATCGTCGACTTGGACATGCTTCTTTCGGCTATCTACGGAGGTTGTTTCCTAGCCTATTTGTTAAGCATGATGTTTCTAGCTTTAAATGTGGTGTTTGTGAACTAGCTAAAAGTCATCGTGCTTCGTTTCCATCCAGTTTGAATAAAAGTTCTGTTCCATTTATGATAATTCATTCTGATGTTTGGGGTCCATCTAAAACTGCTACATTTGGTGGTGCTCATTGGTTCGTTACTTTTATTGATGATTGCACACGTATGACTTGGGTTTGTTTGATGAAATCCAAAGGTGAAGTTACTTCTTTGTTTCAACAGTTTTACAAAATGGTACAAGTACAATATAAGTCACAAATACAGGTTCTCAGGAGTGATAATGGTGGCGAATATGTTAACTCTGAACTTCGTGCCTTTCTTGATCATCATGGGATTGTTCATCAAACTACATGTCCATATACTCCACAACAAAATGGGGCTGCCGAATGCAAGAACCGTCAGCTTCTGGAGGTTGTTCGTGCTTCTTTACTCGAGGCTCGTCTCCCGTTATCCTATTGGGGAGAAGCTCTCACCTCAGCTGCGTATCTCATTAATCGTGTTCCATCCCGATCGGTTGATTTTCAGACACCCCTTCAGGCATTTTCTTTACATGTTGATGCTCCTGCATTCCCTAATCTTCCACCTCATGTGTTTGGATGTGTGGCCTTCGTTCATCTTCATAAACAACAGCGAAGTAAGCTTGAACCTCGAGCCTTACGATGTGTGTTTGTGGGGTATGCATCAAGGCAGAAAGGATACCGTTGTTATCACCCACCGACGAATAAGTTGTTTGTCACTATGGATGTTGCGTTTCATGAGAATGATATGTACTTTGCCAATCTCGAGTCTTCACTTCAGGGGGAGAATCAGAAAGAAGTTCAAACTCTTGATTATACTATTCCAGATATAGATAGAGTGAATGATTTGGATTTAAGTGGTGATGTCTTGGAAATAAGTGGTGATCATTCACACGAAAATAATGATGTGAATGAATTGGAATTAAGTGGTAATGTCTTGGAGACAAGTGGTGATCATTCACATGGAAACAATGTTGAAAACCTTGAAAGGGATGAGTCGACATCGCCAGATAACTCACTGCCTGATAGCTCACTGCCAGTTTCCTCACCACCGGACAACCCTGTGTCGCCAGCTACCTCACAGTCGATCTTGAGTCCCAATAACCATAATCAATCGTCTTCGATCCCGGATGCACCACCACCACGTCGTCTCCCTGATCGCATCAACCGAGGTATTCTTAAACTTACTTATGAAGCCGACCCTAAATGCAAAACTAGGTATCCGAATGGGAAGCTTACTGCACTTATTGTGTATGTAGACGATATGGTGGTAACAGGTAATGATCCGGAAGAACGTGCGGCCTTGCAAAGATACCTGTCTACAGAATTTGAAATGAATGACCTTGGATCCCTGAAATATTTTTTAGGGATTGAGGTGTCGAGAAGCAGTTCTGGAATTTTCTTGTCTCAGAGGAAGTATATTATTGATTTGTTGCACGAAACTGACATGTCAGCTTGTCAGCCAGTAGCTACACCATTAGAAGAAGGATTGAAGCTTAGTGTTGATCCTAATCAAGTACCAATTGACAAAGGAAGATACCAAAGGTTAGTAGGCCGTTTGATGTATTTGGCACACACAAGACTGGACCTTGCTTATGCCTTGAGTGTAGTGAGTAAATACATGCATGATCCTGGAGAACAACATATGAATGCCGTGACGAGAATATTGCGATATTTGAAGGGAAGTCCGGGCAAAGGAATTTTGTTTAAGAGGAATAATCACTTTAGTGTTGAAGGTTATACTGATGCAGACTGGGCAGGTTCCATTGATGATAGACGCTCAACATCAGGTTACTTTACGTTTGTTGGGGGTAACTTGGTTACATGGAGAAGTAAGAAACAGAATGTGGTTGCCCGTTCCAGTGCAGAAGCAGAATACAGAGGTATGGCTTTGGGGATTTGTGAAATTTTGTGGCTTAAGCTTCTGTTAAGCGATTTGGGTATACAACATGATCAGCCCATGAAATTATTTTGTGATAATAAAGATGCTCGTGACATTGCACATAATCCGGTACAACATGATAGAACAAAGCATGTTGAAGTTGATTGGTTCTTCATCAAAGAAAAATTGGAAGGGAAAATAATTGAAGTACCAGCGATAAGAACAGAAGATCAGTTGGCAGATATCCTCACAAAAGCTGTTTCGAGTCACAAGTTTTCAAGTTTCTTACACAAGTTAGGCATGATCGACATATATTCACCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

980

Amino Acids

109.6

Weight (kDa)

6.87

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 67 - 139 2.2e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 173 - 246 5.3e-14 GAG-pre-integrase domain
rve PF00665 264 - 360 5.7e-14 Integrase core domain
SH3_retrovirus PF25597 438 - 498 1.7e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 651 - 738 7.5e-16 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 238, 2480
AccI GTMKAC 5 cut(s) 631, 1730, 1989, 2051, 2674
AccIII TCCGGA 1 cut(s) 2017
AciI CCGC 1 cut(s) 2030
AclWI GGATC 7 cut(s) 83, 1849, 2009, 2073, 2086, 2231, 2354
AcsI RAATTY 5 cut(s) 583, 2057, 2127, 2430, 2637
AcuI CTGAAG 2 cut(s) 1232, 1493
AfaI GTAC 8 cut(s) 112, 252, 951, 957, 1484, 2250, 2745, 2823
AfiI CCNNNNNNNGG 2 cut(s) 1720, 2426
AflII CTTAAG 1 cut(s) 2648
AflIII ACRYGT 4 cut(s) 875, 1064, 1264, 2178
AjiI CACGTC 1 cut(s) 1877
AjnI CCWGG 1 cut(s) 2362
AleI CACNNNNGTG 1 cut(s) 2457
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 6 cut(s) 63, 337, 1160, 1667, 1886, 2142
AlwI GGATC 7 cut(s) 83, 1849, 2009, 2073, 2086, 2231, 2354
AlwNI CAGNNNCTG 1 cut(s) 1120
Ama87I CYCGRG 3 cut(s) 1145, 1362, 1496
Aor13HI TCCGGA 1 cut(s) 2017
AoxI GGCC 4 cut(s) 446, 1317, 2031, 2284
ApeKI GCWGC 2 cut(s) 1094, 1193
ApoI RAATTY 5 cut(s) 583, 2057, 2127, 2430, 2637
ArsI GACNNNNNNTTYG 2 cut(s) 1051, 1083
AseI ATTAAT 1 cut(s) 1206
Asp700I GAANNNNTTC 1 cut(s) 2119
AspS9I GGNCC 3 cut(s) 60, 812, 2317
AsuC2I CCSGG 2 cut(s) 1859, 2421
AsuHPI GGTGA 8 cut(s) 923, 1177, 1415, 1604, 1625, 1694, 1770, 2922
AsuII TTCGAA 1 cut(s) 550
AvaI CYCGRG 3 cut(s) 1145, 1362, 1496
AvaII GGWCC 3 cut(s) 60, 812, 2317
BaeI ACNNNNGTAYC 2 cut(s) 1402, 1435
BamHI GGATCC 1 cut(s) 2078
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BauI CACGAG 1 cut(s) 2721
BbsI GAAGAC 2 cut(s) 1494, 1842
Bbv12I GWGCWC 1 cut(s) 844
BbvCI CCTCAGC 1 cut(s) 1189
BbvI GCAGC 2 cut(s) 1081, 1180
BccI CCATC 3 cut(s) 763, 823, 1225
BceAI ACGGC 2 cut(s) 2271, 2369
BcgI CGANNNNNNTGC 2 cut(s) 2380, 2414
BciT130I CCWGG 1 cut(s) 2364
BciVI GTATCC 2 cut(s) 1403, 1962
BclI TGATCA 4 cut(s) 1036, 1615, 1684, 2683
BcnI CCSGG 2 cut(s) 1859, 2421
BcoDI GTCTC 6 cut(s) 63, 337, 1160, 1667, 1886, 2142
BfaI CTAG 5 cut(s) 243, 675, 702, 728, 1947
BfmI CTRYAG 1 cut(s) 2052
BfrI CTTAAG 1 cut(s) 2648
BfuAI ACCTGC 2 cut(s) 238, 2480
BfuI GTATCC 2 cut(s) 1403, 1962
BisI GCNGC 3 cut(s) 1095, 1194, 2031
BlsI GCNGC 3 cut(s) 1096, 1195, 2032
Bme1390I CCNGG 3 cut(s) 1859, 2364, 2421
Bme18I GGWCC 3 cut(s) 60, 812, 2317
BmeT110I CYCGRG 3 cut(s) 1145, 1362, 1496
BmgBI CACGTC 1 cut(s) 1877
BmgT120I GGNCC 3 cut(s) 60, 812, 2317
BmiI GGNNCC 4 cut(s) 6, 813, 2080, 2494
BmrFI CCNGG 3 cut(s) 1859, 2364, 2421
BmrI ACTGGG 1 cut(s) 2494
BmsI GCATC 8 cut(s) 188, 634, 1261, 1403, 1852, 1902, 2467, 2707
BmuI ACTGGG 1 cut(s) 2494
BpiI GAAGAC 2 cut(s) 1494, 1842
BplI GAGNNNNNCTC 2 cut(s) 1167, 1199
BpmI CTGGAG 2 cut(s) 1142, 2385
Bpu10I CCTNAGC 1 cut(s) 1189
Bpu14I TTCGAA 1 cut(s) 550
BpuEI CTTGAG 2 cut(s) 1843, 2353
BpuMI CCSGG 2 cut(s) 1859, 2421
BsaAI YACGTR 1 cut(s) 878
BsaBI GATNNNNATC 1 cut(s) 2241
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 1900, 2074
BsaWI WCCGGW 5 cut(s) 44, 212, 1783, 2017, 2740
BsaXI ACNNNNNCTCC 4 cut(s) 602, 632, 1866, 1896
Bsc4I CCNNNNNNNGG 2 cut(s) 1720, 2426
Bse1I ACTGG 7 cut(s) 73, 759, 1769, 2195, 2319, 2489, 2592
Bse3DI GCAATG 1 cut(s) 2727
Bse8I GATNNNNATC 1 cut(s) 2241
BseAI TCCGGA 1 cut(s) 2017
BseBI CCWGG 1 cut(s) 2364
BseDI CCNNGG 2 cut(s) 1900, 2074
BseGI GGATG 6 cut(s) 755, 1217, 1316, 1462, 1729, 1867
BseJI GATNNNNATC 1 cut(s) 2241
BseLI CCNNNNNNNGG 2 cut(s) 1720, 2426
BseMI GCAATG 1 cut(s) 2727
BseMII CTCAG 3 cut(s) 997, 1203, 2153
BseNI ACTGG 7 cut(s) 73, 759, 1769, 2195, 2319, 2489, 2592
BseXI GCAGC 2 cut(s) 1081, 1180
BsgI GTGCAG 2 cut(s) 1956, 2616
Bsh1285I CGRYCG 2 cut(s) 67, 1226
BshFI GGCC 4 cut(s) 448, 1319, 2033, 2286
BsiEI CGRYCG 2 cut(s) 67, 1226
BsiHKAI GWGCWC 1 cut(s) 844
BsiHKCI CYCGRG 3 cut(s) 1145, 1362, 1496
BsiSI CCGG 7 cut(s) 45, 213, 1784, 1859, 2018, 2420, 2741
BslFI GGGAC 1 cut(s) 1812
BslI CCNNNNNNNGG 2 cut(s) 1720, 2426
BsmAI GTCTC 6 cut(s) 63, 337, 1160, 1667, 1886, 2142
BsmBI CGTCTC 2 cut(s) 1160, 1886
BsmFI GGGAC 1 cut(s) 1812
BsmI GAATGC 3 cut(s) 1106, 1280, 2386
BsnI GGCC 4 cut(s) 448, 1319, 2033, 2286
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 3 cut(s) 1145, 1362, 1496
Bsp119I TTCGAA 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 844
Bsp13I TCCGGA 1 cut(s) 2017
BspACI CCGC 1 cut(s) 2030
BspANI GGCC 4 cut(s) 448, 1319, 2033, 2286
BspCNI CTCAG 3 cut(s) 996, 1202, 2152
BspEI TCCGGA 1 cut(s) 2017
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 4 cut(s) 6, 813, 2080, 2494
BspMI ACCTGC 2 cut(s) 238, 2480
BspPI GGATC 7 cut(s) 83, 1849, 2009, 2073, 2086, 2231, 2354
BspT104I TTCGAA 1 cut(s) 550
BspTI CTTAAG 1 cut(s) 2648
BspTNI GGTCTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 2727
BsrI ACTGG 7 cut(s) 73, 759, 1769, 2195, 2319, 2489, 2592
BssECI CCNNGG 2 cut(s) 1900, 2074
BssNAI GTATAC 1 cut(s) 2675
BssSI CACGAG 1 cut(s) 2721
BssT1I CCWWGG 1 cut(s) 2074
Bst1107I GTATAC 1 cut(s) 2675
Bst2BI CACGAG 1 cut(s) 2721
Bst2UI CCWGG 1 cut(s) 2364
BstAFI CTTAAG 1 cut(s) 2648
BstBAI YACGTR 1 cut(s) 878
BstBI TTCGAA 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 1803
BstDEI CTNAG 5 cut(s) 350, 983, 1189, 2139, 2228
BstF5I GGATG 6 cut(s) 755, 1217, 1316, 1462, 1729, 1867
BstMAI GTCTC 6 cut(s) 63, 337, 1160, 1667, 1886, 2142
BstMCI CGRYCG 2 cut(s) 67, 1226
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1351, 2486
BstNI CCWGG 1 cut(s) 2364
BstNSI RCATGY 6 cut(s) 644, 1068, 1268, 2182, 2356, 2768
BstSCI CCNGG 3 cut(s) 1857, 2362, 2419
BstSFI CTRYAG 1 cut(s) 2052
BstV1I GCAGC 2 cut(s) 1081, 1180
BstV2I GAAGAC 2 cut(s) 1494, 1842
BstX2I RGATCY 1 cut(s) 2078
BstYI RGATCY 1 cut(s) 2078
BstZ17I GTATAC 1 cut(s) 2675
BsuI GTATCC 2 cut(s) 1403, 1962
BsuRI GGCC 4 cut(s) 448, 1319, 2033, 2286
BtgZI GCGATG 1 cut(s) 1719
BtrI CACGTC 1 cut(s) 1877
BtsCI GGATG 6 cut(s) 755, 1217, 1316, 1462, 1729, 1867
BtsI GCAGTG 3 cut(s) 579, 1748, 1763
BtsIMutI CAGTG 6 cut(s) 94, 327, 579, 1748, 1763, 2599
BveI ACCTGC 2 cut(s) 238, 2480
Cac8I GCNNGC 1 cut(s) 1803
CaiI CAGNNNCTG 1 cut(s) 1120
CciI TCATGA 1 cut(s) 1468
Cfr13I GGNCC 3 cut(s) 60, 812, 2317
CseI GACGC 1 cut(s) 2517
Csp6I GTAC 8 cut(s) 111, 251, 950, 956, 1483, 2249, 2744, 2822
CviQI GTAC 8 cut(s) 111, 251, 950, 956, 1483, 2249, 2744, 2822
DdeI CTNAG 5 cut(s) 350, 983, 1189, 2139, 2228
DraI TTTAAA 2 cut(s) 168, 709
Eco130I CCWWGG 1 cut(s) 2074
Eco31I GGTCTC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 2857
Eco47I GGWCC 3 cut(s) 60, 812, 2317
Eco57I CTGAAG 2 cut(s) 1232, 1493
Eco88I CYCGRG 3 cut(s) 1145, 1362, 1496
EcoRII CCWGG 1 cut(s) 2362
EcoRV GATATC 1 cut(s) 2857
EcoT14I CCWWGG 1 cut(s) 2074
EcoT22I ATGCAT 2 cut(s) 1396, 2358
ErhI CCWWGG 1 cut(s) 2074
Esp3I CGTCTC 2 cut(s) 1160, 1886
FaqI GGGAC 1 cut(s) 1812
FauNDI CATATG 2 cut(s) 228, 2376
FbaI TGATCA 4 cut(s) 1036, 1615, 1684, 2683
FblI GTMKAC 5 cut(s) 631, 1730, 1989, 2051, 2674
Fnu4HI GCNGC 3 cut(s) 1095, 1194, 2031
FokI GGATG 6 cut(s) 742, 1204, 1323, 1469, 1736, 1874
Fsp4HI GCNGC 3 cut(s) 1095, 1194, 2031
FspBI CTAG 5 cut(s) 243, 675, 702, 728, 1947
GluI GCNGC 3 cut(s) 1095, 1194, 2031
GsuI CTGGAG 2 cut(s) 1142, 2385
HaeIII GGCC 4 cut(s) 448, 1319, 2033, 2286
HapII CCGG 7 cut(s) 45, 213, 1784, 1859, 2018, 2420, 2741
HgaI GACGC 1 cut(s) 2517
HincII GTYRAC 4 cut(s) 558, 632, 1012, 1731
HindII GTYRAC 4 cut(s) 558, 632, 1012, 1731
HindIII AAGCTT 5 cut(s) 603, 1352, 1964, 2225, 2651
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 7 cut(s) 45, 213, 1784, 1859, 2018, 2420, 2741
HphI GGTGA 8 cut(s) 923, 1177, 1415, 1604, 1625, 1694, 1770, 2922
Hpy188I TCNGA 8 cut(s) 477, 610, 802, 1018, 1239, 1524, 1956, 2142
Hpy99I CGWCG 3 cut(s) 633, 1435, 1881
HpyCH4IV ACGT 4 cut(s) 877, 1876, 2026, 2531
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1351, 2486
HpyF3I CTNAG 5 cut(s) 350, 983, 1189, 2139, 2228
HpySE526I ACGT 4 cut(s) 877, 1876, 2026, 2531
Kpn2I TCCGGA 1 cut(s) 2017
Ksp22I TGATCA 4 cut(s) 1036, 1615, 1684, 2683
KspAI GTTAAC 1 cut(s) 1012
LmnI GCTCC 3 cut(s) 10, 529, 1279
Lsp1109I GCAGC 2 cut(s) 1081, 1180
LweI GCATC 8 cut(s) 188, 634, 1261, 1403, 1852, 1902, 2467, 2707
MaeI CTAG 5 cut(s) 243, 675, 702, 728, 1947
MaeII ACGT 4 cut(s) 877, 1876, 2026, 2531
MboII GAAGA 9 cut(s) 1283, 1322, 1494, 1842, 2033, 2225, 2277, 2776, 2852
MfeI CAATTG 1 cut(s) 2253
MflI RGATCY 1 cut(s) 2078
MhlI GDGCHC 1 cut(s) 844
MlyI GAGTC 6 cut(s) 472, 553, 1508, 1736, 1834, 2887
Mph1103I ATGCAT 2 cut(s) 1396, 2358
MroI TCCGGA 1 cut(s) 2017
MroXI GAANNNNTTC 1 cut(s) 2119
MslI CAYNNNNRTG 7 cut(s) 801, 878, 1269, 1473, 1692, 2379, 2457
MspA1I CMGCKG 1 cut(s) 1193
MspCI CTTAAG 1 cut(s) 2648
MspI CCGG 7 cut(s) 45, 213, 1784, 1859, 2018, 2420, 2741
MspR9I CCNGG 3 cut(s) 1859, 2364, 2421
MunI CAATTG 1 cut(s) 2253
Mva1269I GAATGC 3 cut(s) 1106, 1280, 2386
MvaI CCWGG 1 cut(s) 2364
MwoI GCNNNNNNNGC 3 cut(s) 11, 1351, 2486
NciI CCSGG 2 cut(s) 1859, 2421
NdeI CATATG 2 cut(s) 228, 2376
NlaIV GGNNCC 4 cut(s) 6, 813, 2080, 2494
NmuCI GTSAC 5 cut(s) 966, 1447, 2386, 2723, 2879
NsiI ATGCAT 2 cut(s) 1396, 2358
NspI RCATGY 6 cut(s) 644, 1068, 1268, 2182, 2356, 2768
NspV TTCGAA 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 2457
PaeR7I CTCGAG 3 cut(s) 1145, 1362, 1496
PagI TCATGA 1 cut(s) 1468
PciI ACATGT 3 cut(s) 1064, 1264, 2178
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 1369, 1897
PctI GAATGC 3 cut(s) 1106, 1280, 2386
PdmI GAANNNNTTC 1 cut(s) 2119
PfeI GAWTC 5 cut(s) 125, 137, 209, 239, 1519
PfoI TCCNGGA 2 cut(s) 1857, 2362
PkrI GCNGC 3 cut(s) 1096, 1195, 2032
Ple19I CGATCG 2 cut(s) 67, 1226
PleI GAGTC 6 cut(s) 472, 553, 1507, 1735, 1833, 2886
PpsI GAGTC 6 cut(s) 472, 553, 1507, 1735, 1833, 2886
Ppu21I YACGTR 1 cut(s) 878
PscI ACATGT 3 cut(s) 1064, 1264, 2178
PshBI ATTAAT 1 cut(s) 1206
Psp6I CCWGG 1 cut(s) 2362
PspGI CCWGG 1 cut(s) 2362
PspN4I GGNNCC 4 cut(s) 6, 813, 2080, 2494
PspPI GGNCC 3 cut(s) 60, 812, 2317
PspXI VCTCGAGB 2 cut(s) 1145, 1362
PstNI CAGNNNCTG 1 cut(s) 1120
PsuI RGATCY 1 cut(s) 2078
PvuI CGATCG 2 cut(s) 67, 1226
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 8 cut(s) 112, 252, 951, 957, 1484, 2250, 2745, 2823
RsaNI GTAC 8 cut(s) 111, 251, 950, 956, 1483, 2249, 2744, 2822
RseI CAYNNNNRTG 7 cut(s) 801, 878, 1269, 1473, 1692, 2379, 2457
SalI GTCGAC 2 cut(s) 630, 1729
SatI GCNGC 3 cut(s) 1095, 1194, 2031
Sau96I GGNCC 3 cut(s) 60, 812, 2317
SchI GAGTC 6 cut(s) 472, 553, 1508, 1736, 1834, 2887
ScrFI CCNGG 3 cut(s) 1859, 2364, 2421
SduI GDGCHC 1 cut(s) 844
SfaNI GCATC 8 cut(s) 188, 634, 1261, 1403, 1852, 1902, 2467, 2707
SfcI CTRYAG 1 cut(s) 2052
Sfr274I CTCGAG 3 cut(s) 1145, 1362, 1496
SfuI TTCGAA 1 cut(s) 550
SinI GGWCC 3 cut(s) 60, 812, 2317
SlaI CTCGAG 3 cut(s) 1145, 1362, 1496
SmiMI CAYNNNNRTG 7 cut(s) 801, 878, 1269, 1473, 1692, 2379, 2457
SmlI CTYRAG 6 cut(s) 1145, 1362, 1496, 1822, 2332, 2648
SmoI CTYRAG 6 cut(s) 1145, 1362, 1496, 1822, 2332, 2648
SsiI CCGC 1 cut(s) 2030
SspI AATATT 2 cut(s) 2090, 2397
SspMI CTAG 5 cut(s) 243, 675, 702, 728, 1947
StyD4I CCNGG 3 cut(s) 1857, 2362, 2419
StyI CCWWGG 1 cut(s) 2074
TaiI ACGT 4 cut(s) 880, 1879, 2029, 2534
TaqII GACCGA 2 cut(s) 54, 77
TatI WGTACW 2 cut(s) 955, 1482
TauI GCSGC 1 cut(s) 2033
TfiI GAWTC 5 cut(s) 125, 137, 209, 239, 1519
TscAI CASTG 6 cut(s) 101, 334, 579, 1755, 1770, 2599
TseFI GTSAC 5 cut(s) 966, 1447, 2386, 2723, 2879
TseI GCWGC 2 cut(s) 1094, 1193
Tsp45I GTSAC 5 cut(s) 966, 1447, 2386, 2723, 2879
TspGWI ACGGA 2 cut(s) 594, 676
TspRI CASTG 6 cut(s) 101, 334, 579, 1755, 1770, 2599
Vha464I CTTAAG 1 cut(s) 2648
VpaK11BI GGWCC 3 cut(s) 60, 812, 2317
VspI ATTAAT 1 cut(s) 1206
XapI RAATTY 5 cut(s) 583, 2057, 2127, 2430, 2637
XceI RCATGY 6 cut(s) 644, 1068, 1268, 2182, 2356, 2768
XhoI CTCGAG 3 cut(s) 1145, 1362, 1496
XmiI GTMKAC 5 cut(s) 631, 1730, 1989, 2051, 2674
XmnI GAANNNNTTC 1 cut(s) 2119
XspI CTAG 5 cut(s) 243, 675, 702, 728, 1947
Zsp2I ATGCAT 2 cut(s) 1396, 2358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.