MD11G1107300.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000071
Physical Location & Seq
Forward (+)
9475151 .. 9479452
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1107300.v1.1.491

Sequence Viewer

Length: 4149 bp
ATGACTGAAGAAAGTTCTGTGAACCATGATGAGGAAACCCGGCACAATCTCTCTCCAAACTTCTCAGACGTTGAGGTTAACACCAATCAACGTTTGTGTTCAGTTCTCTTGAACGAGTTCAATTATCTTCCTTGGTCCCGAGCTGTTTCACTAGCTCTCGGAGGCAAAGGGAAGTTAGGGTTTGTAAATGGAAGCGTGGAAGCTCCAGACAGTTCTTCCTCTACATACAATGCATGGCTCTGCAAAGATCAACTTGTCATGTCATTGCTGCTCAACACCATGGAGAAACATGTTGCTGAAATTTTTAGCTACTCTAATTCGGCACATGATCTTTGGAAAGCTTTGCAGGATATGTATGGAAATCAAAACAACTATGCACGGGTCTTCCAATTAAAGAAGGACATTGCCAGTGCTCAACAAGAAGGGAAAGCATTTGTTCAACACCTTGGGAGCCTCAAAGCCATGTGGAATGAGTTGGATGTATATCTCCCTCATACCACAGATCCTACCATTCTCCTAAAACGAGCTGAGGAAGACAAAATTTATCAGCTGTTAGGGAGTTTGAGCTCTGAGTACGAGGACCTAAGGAGTCACATCTTGATGAGTCAAGACCTGCCTACCTTCAACAATGTTTGTGCAACTATCCAACGAGAAGAAGCAAGGAAGAAGGTTATGAATGTTGATCACAATCCTAGATTAACGGAGACTCGGGCATATGCATCAAAGTACAAGAACTCAGAAGCCAAGGTATACAAGGGAAGAAACACACATCTAAAATGCAAATATTGCAATGGTGTTGGTCATGAGGAAGACAAGTGTTGGGAACTTCATCCTGAACTCAAGCCTAAGTTCAGCAAAGATGGAAGGATGATACCAAGGTCCTCACAACAATTTCAACCTCATTTCAAGGCACAACTTGCTAATGCTCACACTCCTACTATCTCACAAGGATCCATGGAGTTCACTGCCAATCCATTGTCATTGATCAATGAATTTGCAGCTTACCTACAAAGTAAGGGGCACGGAGAAGACTCACATGGTCAAGGCAATGAAGAGGGTAGTCACACAGCTATGTTGGGACAATTTGCCGGATTTCTTGCTGGAAATGAGAAAGTTCCAAAGGGAGAAGCATCAGGTATACTCAAAGCCTTTACTACTGCCCTATTAACTAGTACTGAAATTGATTGTTGGATAATTGATTCAGGTCCCACAGATCATATGACAAACAAAGTTACAAATTTACATCATTTTAAAAAAATTTCAAGCCCCTCACATGTGTCTGTTGCAAATGGAAAAAATGTCTTAGTTTTGGGAAAAGGAGAAATAAACTTACTCTCTCAAAAAACACCTTCCACTGCCCTATATGTACCAACCTTTCCCTTCCAACTTTTGTCAATTGGAAAGATCACCAACACCCTAAACTGCCGTGCTATTTTCTCACCAAATAAAGTAATGTTTCAGGATGTTCTCACCATGAAGATGATTGGTGAAGGGGTCTTCATAAATGGACTTTACTACCTATGCAAGAATGCATGTTTCACTCAGGCTCTTCAAGCTCAATCGAAGTCCATAGATGAAAACCAACTTTGGCATAGGAGATTAGCTCACCCCTCTGAACTTGTCTTGTCGAAACTGTTTCCAAATTTTTGTAACCCCTATCCTACTTGTGATACTTGTCAATTTTCTAAGTTTACTAGGCTACCTTTTGGTAATTCAATGTCTAGGACAAGTAAACCTTTTGAAATGGTACACACCGATGTATGGGGACCTGCAACTGAATCAATGGAAGATAATGGCACTGAATTTCTATCCAACAACATGCTTCAATACATAAGTTCTCAGGGTATCATACATCAAACCAGCTGTGTGGGGACTCCCCAACAAAATGGAGTAGCCGAGAGGAAAAATAGAGATCTCTTGGAAAAAACTCGTGCTCTCATGATTCACATGCACGTTCCAAAGAAATTTTGGTCGTTTGCCATCCTTACTGCCACTTATCTCATCAATCGACTTCCAAGTCGTGTGCTAGGGTTTAAATCTCCCTATGAAGTTCTCAAGGGGAGGAAAATAGATTTGACACATCTCAAGGTGTTTGGTTGTGTGTGTTTTGTTCACATCCAAACCTTGAACCATGACAAACTAGATGCCAGGGCAACCAAGTGTGTGTTTATGGGATACTCGACCACTCAAAATGGATACAAGTGCTTCAATCCAGTCACTAAGAAGTGCACAATTTCAAGAGATGTGAAGTTCGAAGAAGACTATCCCTATTTTACAAGTCAGAGGGAGGATTTAGTTGACATGTTCCCACTCCCTCAAAACTTTAATTATCCGATGTTGGAAAAGAGATCAGTCATTGTAAGTCCAGATTGTGAAGAAGTTCAAACTGACCAAATTACTGCCAGTGAGAGTGAAGACGAGCCCTACTCTGATCCTTCTCCGCCCTCTACAGAGTCTCAGGTGATTAAAAGAAATCATCCTCGAATCAGAAAACCTCCAGCTAGGTTGCAGGACTATGTTACATATGCCTCACGACATCCAATCAATGAATGTATCAGCTTTAGTAAGTTCTCAACATCTCATGCTGCATTTCTCAGTGAAATTGATAAGCACTATGAACCAAGAAGCTTTCAAGAAGCTTCTCTTCTCCCACAATGGAACCAAGCCATGAAAGAAGAGATTCGTGCACTGGAAGAGAATTGCACCTGGAGCCTAGTTCAACTACCACCAGGAAAAAAGGCTATTGGAAGTCGTTGGATTTACAAGACTAAATTCAAAGCTGATGGATCTATCGAGAGACACAAGGCTAGACTTGTTGCTCGAGGTTTCACCCAAACCTTTGGTGTAGATTATAAGGAAACATTCGCACCGGTGGCCAAGATGAACTCAGTCAGGGTTTTACTATCAGTTGCAATCAATTGTGGATGGTCACTCTACCAAATGGACGTGAAGAATGCTTTCCTTCACGACGAGCTGCAGGAAGAAGTGTATATGCAACCTCCTCCAGGCTATGATGGTATTAAAGGCAACATGGTTTGTAAATTGCACAAGGCAATATACGGATTGAAACAATCCCCAAGAGCTTGGTATGCCAAGCTCAGTTCTGTTCTGGAAAAGGCTGGATTCATGCGAAGTAATGCTGATTCTTCGTTATTTGTAAGAACTGGCACCAGGGGGAAGTTGGTGGTCCTCGTCTATGTTGATGATCTTATCATCACTGGAGATAATACCGTGGAGATTGAGGCACTAAAACTCTCACTACATCAAGCTTTCGCTATCAAAGTTTTGGGGAGGTTAAAGTATTTTTTGGGGATCGAAATGTCAACATCTTCAAAAGGACTGTTTCTACATCAACGGAAATATGTATTGGACCTCCTTCAAGAAGCAAAAATGCTTGACTGCAAGCCTGCTATCACTCCCGTTGATTGTAAACTGAAGCTCAACATAGATGGAGAAGCCATGCATGATGTAAGCTACTATCAACGATTAGTAGGCAAGCTCATATACCTCACTATCACTCGTCCATATATCACATATGCAGTGAGCTTGGCTAGTCAGTTCATGCACTCTCCCACTGTTGATCACCTTAACCTTGTTAAGAGAATCCTTCGTTATCTTAAGGGTTCAATTGGGCAAGGAATTACAATGCATAACAATCAGTCCACTGTCATTAGTGGCTACACAGATGCAGACTGGGCAGGCAATGCAATTGATAGGAAATCAATCACAGGCTATTGTACATTTGTTGGTGGAAACCTTGTCACATGGAAGAGTAAGAAGCAACAGGTAATTGCTCGTTCCAGCGCAGAGGCTGAGTATCGAGCTATGGCAGCCACTGCGTGTGAACTCATTTGGCTTAAAGGGCTTCTTTCAGACTTAGGGTTTCCTAGTTCTACTCCTATGACGCTTATGTGTGATAATCAAGCAGCCATGCACATTGCTGCCAATCTTGTGTTCCATGAAAGAACCAAACATATTGAACTGGATTGTCATTTCATCAGAGCTCAAGTTCAAACGCAAATCATCCGGACCATGTTCACACGAAGCCATGATCAACTAGCAGATCTTTTTACAAAGGCACTAGCATCATCTCAGTTTCATCGGTTATTGGGCAAGCTTGGCTCAGTGAATCCCCTCGATCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1383

Amino Acids

155.91

Weight (kDa)

8.7

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 31 - 70 7e-13 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 53 - 224 8.1e-16 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 397 - 471 1.4e-12 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 503 - 563 3.7e-09 GAG-pre-integrase domain
SH3_retrovirus PF25597 700 - 757 1.7e-23 Retroviral polymerase SH3-like domain
RVT_2 PF07727 901 - 1141 4.9e-75 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2853
AasI GACNNNNNNGTC 1 cut(s) 2016
Acc36I ACCTGC 2 cut(s) 621, 1777
AccB1I GGYRCC 1 cut(s) 3167
AccI GTMKAC 2 cut(s) 750, 1138
AccIII TCCGGA 1 cut(s) 4026
AciI CCGC 1 cut(s) 2441
AclI AACGTT 1 cut(s) 91
AclWI GGATC 7 cut(s) 497, 945, 958, 2426, 2794, 3320, 4133
AcoI YGGCCR 1 cut(s) 2874
AcsI RAATTY 9 cut(s) 300, 540, 992, 1237, 1257, 1642, 1802, 1964, 2771
AcuI CTGAAG 2 cut(s) 27, 3455
AdeI CACNNNGTG 1 cut(s) 3840
AfaI GTAC 6 cut(s) 575, 728, 1174, 1368, 1749, 3739
AfiI CCNNNNNNNGG 3 cut(s) 31, 1761, 3005
AflII CTTAAG 1 cut(s) 3617
AflIII ACRYGT 3 cut(s) 289, 1273, 2301
AgeI ACCGGT 1 cut(s) 2869
AhlI ACTAGT 1 cut(s) 1169
AjiI CACGTC 1 cut(s) 2947
AjnI CCWGG 5 cut(s) 2147, 2705, 2728, 3004, 3170
AjuI GAANNNNNNNTTGG 4 cut(s) 3350, 3382, 3938, 3970
Alw21I GWGCWC 6 cut(s) 415, 569, 1936, 2231, 2689, 4006
Alw26I GTCTC 3 cut(s) 698, 2460, 2791
Alw44I GTGCAC 2 cut(s) 2227, 2685
AlwI GGATC 7 cut(s) 497, 945, 958, 2426, 2794, 3320, 4133
AlwNI CAGNNNCTG 2 cut(s) 3812, 3836
Ama87I CYCGRG 3 cut(s) 138, 708, 2820
Aor13HI TCCGGA 1 cut(s) 4026
AoxI GGCC 1 cut(s) 2874
ApaLI GTGCAC 2 cut(s) 2227, 2685
ApeKI GCWGC 7 cut(s) 268, 998, 2585, 2974, 3830, 3926, 3941
ApoI RAATTY 9 cut(s) 300, 540, 992, 1237, 1257, 1642, 1802, 1964, 2771
AsiGI ACCGGT 1 cut(s) 2869
Asp700I GAANNNNTTC 4 cut(s) 116, 2379, 2679, 2957
AspLEI GCGC 1 cut(s) 3806
AspS9I GGNCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 8 cut(s) 1399, 1431, 1462, 1499, 1598, 2473, 2821, 3575
AsuII TTCGAA 1 cut(s) 2253
AvaI CYCGRG 3 cut(s) 138, 708, 2820
AvaII GGWCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
AxyI CCTNAGG 1 cut(s) 584
BaeGI GKGCMC 3 cut(s) 1023, 2231, 2689
BaeI ACNNNNGTAYC 2 cut(s) 863, 896
BalI TGGCCA 1 cut(s) 2876
BamHI GGATCC 1 cut(s) 950
BanI GGYRCC 1 cut(s) 3167
BanII GRGCYC 3 cut(s) 569, 2424, 4006
BauI CACGAG 1 cut(s) 1929
BbsI GAAGAC 7 cut(s) 376, 540, 816, 1035, 1489, 2265, 2421
Bbv12I GWGCWC 6 cut(s) 415, 569, 1936, 2231, 2689, 4006
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 7 cut(s) 255, 1010, 2572, 2961, 3842, 3928, 3938
BccI CCATC 6 cut(s) 854, 1988, 2777, 2919, 3008, 3443
BceAI ACGGC 1 cut(s) 1410
BciT130I CCWGG 5 cut(s) 2149, 2707, 2730, 3006, 3172
BciVI GTATCC 2 cut(s) 2168, 2189
BclI TGATCA 4 cut(s) 682, 984, 3580, 4051
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 3 cut(s) 698, 2460, 2791
BcuI ACTAGT 1 cut(s) 1169
BfmI CTRYAG 2 cut(s) 2448, 2975
BfrI CTTAAG 1 cut(s) 3617
BfuAI ACCTGC 2 cut(s) 621, 1777
BfuI GTATCC 2 cut(s) 2168, 2189
BglII AGATCT 2 cut(s) 1912, 4063
BisI GCNGC 7 cut(s) 269, 999, 2586, 2975, 3831, 3927, 3942
BlsI GCNGC 7 cut(s) 270, 1000, 2587, 2976, 3832, 3928, 3943
BmcAI AGTACT 1 cut(s) 1174
Bme1390I CCNGG 6 cut(s) 40, 2149, 2707, 2730, 3006, 3172
Bme18I GGWCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
BmeT110I CYCGRG 3 cut(s) 138, 708, 2820
BmgBI CACGTC 1 cut(s) 2947
BmgT120I GGNCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
BmiI GGNNCC 8 cut(s) 137, 452, 952, 1207, 1767, 2660, 2711, 3169
BmrFI CCNGG 6 cut(s) 40, 2149, 2707, 2730, 3006, 3172
BmrI ACTGGG 1 cut(s) 3703
BmsI GCATC 5 cut(s) 728, 1139, 2134, 3676, 4094
BmuI ACTGGG 1 cut(s) 3703
BpiI GAAGAC 7 cut(s) 376, 540, 816, 1035, 1489, 2265, 2421
BplI GAGNNNNNCTC 4 cut(s) 1588, 1620, 2411, 2443
BpmI CTGGAG 5 cut(s) 189, 2481, 2728, 2988, 3240
Bpu10I CCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 2253
BpuEI CTTGAG 4 cut(s) 824, 2039, 2069, 3990
BpuMI CCSGG 1 cut(s) 40
BsaBI GATNNNNATC 2 cut(s) 483, 687
BsaJI CCNNGG 9 cut(s) 131, 279, 445, 744, 875, 954, 2148, 3171, 3231
BsaWI WCCGGW 2 cut(s) 2869, 4026
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 1761, 3005
Bse118I RCCGGY 1 cut(s) 2869
Bse1I ACTGG 8 cut(s) 408, 2213, 2403, 2694, 3169, 3223, 3698, 3987
Bse21I CCTNAGG 1 cut(s) 584
Bse3DI GCAATG 6 cut(s) 263, 402, 796, 1054, 3709, 3936
Bse8I GATNNNNATC 2 cut(s) 483, 687
BseAI TCCGGA 1 cut(s) 4026
BseBI CCWGG 5 cut(s) 2149, 2707, 2730, 3006, 3172
BseDI CCNNGG 9 cut(s) 131, 279, 445, 744, 875, 954, 2148, 3171, 3231
BseJI GATNNNNATC 2 cut(s) 483, 687
BseLI CCNNNNNNNGG 3 cut(s) 31, 1761, 3005
BseMI GCAATG 6 cut(s) 263, 402, 796, 1054, 3709, 3936
BseNI ACTGG 8 cut(s) 408, 2213, 2403, 2694, 3169, 3223, 3698, 3987
BseRI GAGGAG 1 cut(s) 2991
BseSI GKGCMC 3 cut(s) 1023, 2231, 2689
BseXI GCAGC 7 cut(s) 255, 1010, 2572, 2961, 3842, 3928, 3938
BshFI GGCC 1 cut(s) 2876
BshNI GGYRCC 1 cut(s) 3167
BshTI ACCGGT 1 cut(s) 2869
BsiHKAI GWGCWC 6 cut(s) 415, 569, 1936, 2231, 2689, 4006
BsiHKCI CYCGRG 3 cut(s) 138, 708, 2820
BsiSI CCGG 4 cut(s) 40, 1089, 2870, 4027
BslFI GGGAC 5 cut(s) 121, 1092, 1191, 1779, 1885
BslI CCNNNNNNNGG 3 cut(s) 31, 1761, 3005
BsmAI GTCTC 3 cut(s) 698, 2460, 2791
BsmFI GGGAC 5 cut(s) 121, 1092, 1191, 1779, 1885
BsmI GAATGC 2 cut(s) 1534, 2959
BsnI GGCC 1 cut(s) 2876
BsoBI CYCGRG 3 cut(s) 138, 708, 2820
Bsp119I TTCGAA 1 cut(s) 2253
Bsp1286I GDGCHC 8 cut(s) 415, 569, 1023, 1936, 2231, 2424, 2689, 4006
Bsp13I TCCGGA 1 cut(s) 4026
Bsp1407I TGTACA 1 cut(s) 3737
Bsp19I CCATGG 2 cut(s) 279, 954
BspACI CCGC 1 cut(s) 2441
BspANI GGCC 1 cut(s) 2876
BspEI TCCGGA 1 cut(s) 4026
BspHI TCATGA 2 cut(s) 802, 1938
BspLI GGNNCC 8 cut(s) 137, 452, 952, 1207, 1767, 2660, 2711, 3169
BspMAI CTGCAG 1 cut(s) 2979
BspMI ACCTGC 2 cut(s) 621, 1777
BspPI GGATC 7 cut(s) 497, 945, 958, 2426, 2794, 3320, 4133
BspQI GCTCTTC 1 cut(s) 1554
BspT104I TTCGAA 1 cut(s) 2253
BspT107I GGYRCC 1 cut(s) 3167
BspTI CTTAAG 1 cut(s) 3617
BsrDI GCAATG 6 cut(s) 263, 402, 796, 1054, 3709, 3936
BsrFI RCCGGY 1 cut(s) 2869
BsrGI TGTACA 1 cut(s) 3737
BsrI ACTGG 8 cut(s) 408, 2213, 2403, 2694, 3169, 3223, 3698, 3987
BssAI RCCGGY 1 cut(s) 2869
BssECI CCNNGG 9 cut(s) 131, 279, 445, 744, 875, 954, 2148, 3171, 3231
BssNAI GTATAC 2 cut(s) 751, 1139
BssSI CACGAG 1 cut(s) 1929
BssT1I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 954
Bst1107I GTATAC 2 cut(s) 751, 1139
Bst2BI CACGAG 1 cut(s) 1929
Bst2UI CCWGG 5 cut(s) 2149, 2707, 2730, 3006, 3172
Bst4CI ACNGT 6 cut(s) 212, 1635, 3232, 3342, 3577, 3667
Bst6I CTCTTC 6 cut(s) 1047, 1554, 2649, 2670, 2688, 3764
BstAFI CTTAAG 1 cut(s) 3617
BstAPI GCANNNNNTGC 4 cut(s) 786, 917, 3704, 3836
BstAUI TGTACA 1 cut(s) 3737
BstBI TTCGAA 1 cut(s) 2253
BstC8I GCNNGC 5 cut(s) 3404, 3408, 3497, 3700, 4115
BstDSI CCRYGG 3 cut(s) 279, 954, 3231
BstENI CCTNNNNNAGG 1 cut(s) 3003
BstHHI GCGC 1 cut(s) 3806
BstMAI GTCTC 3 cut(s) 698, 2460, 2791
BstNI CCWGG 5 cut(s) 2149, 2707, 2730, 3006, 3172
BstNSI RCATGY 6 cut(s) 293, 1277, 1536, 1822, 1951, 2305
BstSCI CCNGG 6 cut(s) 38, 2147, 2705, 2728, 3004, 3170
BstSFI CTRYAG 2 cut(s) 2448, 2975
BstSLI GKGCMC 3 cut(s) 1023, 2231, 2689
BstV1I GCAGC 7 cut(s) 255, 1010, 2572, 2961, 3842, 3928, 3938
BstV2I GAAGAC 7 cut(s) 376, 540, 816, 1035, 1489, 2265, 2421
BstX2I RGATCY 5 cut(s) 502, 950, 1912, 2786, 4063
BstXI CCANNNNNNTGG 4 cut(s) 1867, 1886, 2840, 3084
BstYI RGATCY 5 cut(s) 502, 950, 1912, 2786, 4063
BstZ17I GTATAC 2 cut(s) 751, 1139
Bsu36I CCTNAGG 1 cut(s) 584
BsuI GTATCC 2 cut(s) 2168, 2189
BsuRI GGCC 1 cut(s) 2876
BtgI CCRYGG 3 cut(s) 279, 954, 3231
BtrI CACGTC 1 cut(s) 2947
BtsI GCAGTG 4 cut(s) 963, 1353, 3546, 3834
BveI ACCTGC 2 cut(s) 621, 1777
Cac8I GCNNGC 5 cut(s) 3404, 3408, 3497, 3700, 4115
CaiI CAGNNNCTG 2 cut(s) 3812, 3836
CciI TCATGA 2 cut(s) 802, 1938
CfoI GCGC 1 cut(s) 3806
Cfr10I RCCGGY 1 cut(s) 2869
Cfr13I GGNCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
CseI GACGC 1 cut(s) 3913
Csp6I GTAC 6 cut(s) 574, 727, 1173, 1367, 1748, 3738
CspAI ACCGGT 1 cut(s) 2869
CviQI GTAC 6 cut(s) 574, 727, 1173, 1367, 1748, 3738
DraI TTTAAA 2 cut(s) 1252, 2035
DraIII CACNNNGTG 1 cut(s) 3840
DrdI GACNNNNNNGTC 1 cut(s) 2016
DseDI GACNNNNNNGTC 1 cut(s) 2016
EaeI YGGCCR 1 cut(s) 2874
Eam1104I CTCTTC 6 cut(s) 1047, 1554, 2649, 2670, 2688, 3764
EarI CTCTTC 6 cut(s) 1047, 1554, 2649, 2670, 2688, 3764
EciI GGCGGA 1 cut(s) 2430
Ecl136II GAGCTC 2 cut(s) 567, 4004
Eco130I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 954
Eco24I GRGCYC 3 cut(s) 569, 2424, 4006
Eco47I GGWCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
Eco53kI GAGCTC 2 cut(s) 567, 4004
Eco57I CTGAAG 2 cut(s) 27, 3455
Eco81I CCTNAGG 1 cut(s) 584
Eco88I CYCGRG 3 cut(s) 138, 708, 2820
EcoICRI GAGCTC 2 cut(s) 567, 4004
EcoNI CCTNNNNNAGG 1 cut(s) 3003
EcoO109I RGGNCCY 4 cut(s) 580, 879, 1205, 1766
EcoRII CCWGG 5 cut(s) 2147, 2705, 2728, 3004, 3170
EcoT14I CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 954
EcoT22I ATGCAT 5 cut(s) 235, 721, 1534, 3465, 3651
EcoT38I GRGCYC 3 cut(s) 569, 2424, 4006
ErhI CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 954
FalI AAGNNNNNCTT 8 cut(s) 237, 269, 1720, 1752, 2628, 2660, 3852, 3884
FaqI GGGAC 5 cut(s) 121, 1092, 1191, 1779, 1885
FauNDI CATATG 4 cut(s) 715, 1218, 2524, 3535
FbaI TGATCA 4 cut(s) 682, 984, 3580, 4051
FblI GTMKAC 2 cut(s) 750, 1138
Fnu4HI GCNGC 7 cut(s) 269, 999, 2586, 2975, 3831, 3927, 3942
FriOI GRGCYC 3 cut(s) 569, 2424, 4006
Fsp4HI GCNGC 7 cut(s) 269, 999, 2586, 2975, 3831, 3927, 3942
GlaI GCGC 1 cut(s) 3805
GluI GCNGC 7 cut(s) 269, 999, 2586, 2975, 3831, 3927, 3942
GsuI CTGGAG 5 cut(s) 189, 2481, 2728, 2988, 3240
HaeIII GGCC 1 cut(s) 2876
HapII CCGG 4 cut(s) 40, 1089, 2870, 4027
HgaI GACGC 1 cut(s) 3913
HhaI GCGC 1 cut(s) 3806
Hin6I GCGC 1 cut(s) 3804
HinP1I GCGC 1 cut(s) 3804
HincII GTYRAC 3 cut(s) 79, 2299, 3324
HindII GTYRAC 3 cut(s) 79, 2299, 3324
HindIII AAGCTT 5 cut(s) 339, 2626, 2637, 3267, 4115
HpaI GTTAAC 1 cut(s) 79
HpaII CCGG 4 cut(s) 40, 1089, 2870, 4027
HphI GGTGA 8 cut(s) 1399, 1431, 1462, 1499, 1598, 2473, 2821, 3575
Hpy99I CGWCG 1 cut(s) 2972
HpyCH4III ACNGT 6 cut(s) 212, 1635, 3232, 3342, 3577, 3667
HpyCH4IV ACGT 4 cut(s) 69, 91, 1953, 2946
HpySE526I ACGT 4 cut(s) 69, 91, 1953, 2946
HspAI GCGC 1 cut(s) 3804
Kpn2I TCCGGA 1 cut(s) 4026
Ksp22I TGATCA 4 cut(s) 682, 984, 3580, 4051
KspAI GTTAAC 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 1554
LmnI GCTCC 3 cut(s) 208, 450, 2709
Lsp1109I GCAGC 7 cut(s) 255, 1010, 2572, 2961, 3842, 3928, 3938
LweI GCATC 5 cut(s) 728, 1139, 2134, 3676, 4094
MaeII ACGT 4 cut(s) 69, 91, 1953, 2946
MaeIII GTNAC 8 cut(s) 590, 1061, 1231, 1649, 2215, 2518, 2928, 3760
MfeI CAATTG 4 cut(s) 1395, 2917, 3627, 3708
MflI RGATCY 5 cut(s) 502, 950, 1912, 2786, 4063
MhlI GDGCHC 8 cut(s) 415, 569, 1023, 1936, 2231, 2424, 2689, 4006
MlsI TGGCCA 1 cut(s) 2876
MluNI TGGCCA 1 cut(s) 2876
MlyI GAGTC 6 cut(s) 598, 613, 700, 1025, 1867, 2462
MmeI TCCRAC 7 cut(s) 456, 670, 1169, 1408, 1836, 2319, 2735
Mox20I TGGCCA 1 cut(s) 2876
Mph1103I ATGCAT 5 cut(s) 235, 721, 1534, 3465, 3651
MroI TCCGGA 1 cut(s) 4026
MroXI GAANNNNTTC 4 cut(s) 116, 2379, 2679, 2957
MscI TGGCCA 1 cut(s) 2876
MslI CAYNNNNRTG 4 cut(s) 599, 1070, 1478, 1755
Msp20I TGGCCA 1 cut(s) 2876
MspA1I CMGCKG 2 cut(s) 550, 1863
MspCI CTTAAG 1 cut(s) 3617
MspI CCGG 4 cut(s) 40, 1089, 2870, 4027
MspR9I CCNGG 6 cut(s) 40, 2149, 2707, 2730, 3006, 3172
MunI CAATTG 4 cut(s) 1395, 2917, 3627, 3708
Mva1269I GAATGC 2 cut(s) 1534, 2959
MvaI CCWGG 5 cut(s) 2149, 2707, 2730, 3006, 3172
NciI CCSGG 1 cut(s) 40
NcoI CCATGG 2 cut(s) 279, 954
NdeI CATATG 4 cut(s) 715, 1218, 2524, 3535
NlaIV GGNNCC 8 cut(s) 137, 452, 952, 1207, 1767, 2660, 2711, 3169
NmeAIII GCCGAG 1 cut(s) 1921
NmuCI GTSAC 5 cut(s) 590, 1061, 2215, 2928, 3760
NsiI ATGCAT 5 cut(s) 235, 721, 1534, 3465, 3651
NspI RCATGY 6 cut(s) 293, 1277, 1536, 1822, 1951, 2305
NspV TTCGAA 1 cut(s) 2253
PaeR7I CTCGAG 1 cut(s) 2820
PagI TCATGA 2 cut(s) 802, 1938
PciI ACATGT 3 cut(s) 289, 1273, 2301
PciSI GCTCTTC 1 cut(s) 1554
PctI GAATGC 2 cut(s) 1534, 2959
PdmI GAANNNNTTC 4 cut(s) 116, 2379, 2679, 2957
PfeI GAWTC 9 cut(s) 1199, 1778, 1942, 2484, 2680, 3123, 3143, 3603, 4129
PinAI ACCGGT 1 cut(s) 2869
PkrI GCNGC 7 cut(s) 270, 1000, 2587, 2976, 3832, 3928, 3943
PleI GAGTC 6 cut(s) 597, 612, 700, 1025, 1867, 2461
PpsI GAGTC 6 cut(s) 597, 612, 700, 1025, 1867, 2461
PpuMI RGGWCCY 4 cut(s) 580, 879, 1205, 1766
PscI ACATGT 3 cut(s) 289, 1273, 2301
PsiI TTATAA 1 cut(s) 2853
Psp124BI GAGCTC 2 cut(s) 569, 4006
Psp1406I AACGTT 1 cut(s) 91
Psp5II RGGWCCY 4 cut(s) 580, 879, 1205, 1766
Psp6I CCWGG 5 cut(s) 2147, 2705, 2728, 3004, 3170
PspGI CCWGG 5 cut(s) 2147, 2705, 2728, 3004, 3170
PspN4I GGNNCC 8 cut(s) 137, 452, 952, 1207, 1767, 2660, 2711, 3169
PspPI GGNCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
PspPPI RGGWCCY 4 cut(s) 580, 879, 1205, 1766
PspXI VCTCGAGB 1 cut(s) 2820
PstI CTGCAG 1 cut(s) 2979
PstNI CAGNNNCTG 2 cut(s) 3812, 3836
PsuI RGATCY 5 cut(s) 502, 950, 1912, 2786, 4063
PvuII CAGCTG 2 cut(s) 550, 1863
RsaI GTAC 6 cut(s) 575, 728, 1174, 1368, 1749, 3739
RsaNI GTAC 6 cut(s) 574, 727, 1173, 1367, 1748, 3738
RseI CAYNNNNRTG 4 cut(s) 599, 1070, 1478, 1755
SacI GAGCTC 2 cut(s) 569, 4006
SapI GCTCTTC 1 cut(s) 1554
SatI GCNGC 7 cut(s) 269, 999, 2586, 2975, 3831, 3927, 3942
Sau96I GGNCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
ScaI AGTACT 1 cut(s) 1174
SchI GAGTC 6 cut(s) 598, 613, 700, 1025, 1867, 2462
ScrFI CCNGG 6 cut(s) 40, 2149, 2707, 2730, 3006, 3172
SduI GDGCHC 8 cut(s) 415, 569, 1023, 1936, 2231, 2424, 2689, 4006
SfaNI GCATC 5 cut(s) 728, 1139, 2134, 3676, 4094
SfcI CTRYAG 2 cut(s) 2448, 2975
Sfr274I CTCGAG 1 cut(s) 2820
SfuI TTCGAA 1 cut(s) 2253
SgrAI CRCCGGYG 1 cut(s) 2869
SinI GGWCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
SlaI CTCGAG 1 cut(s) 2820
SmiMI CAYNNNNRTG 4 cut(s) 599, 1070, 1478, 1755
SmlI CTYRAG 6 cut(s) 839, 2054, 2084, 2820, 3617, 4005
SmoI CTYRAG 6 cut(s) 839, 2054, 2084, 2820, 3617, 4005
SpeI ACTAGT 1 cut(s) 1169
SsiI CCGC 1 cut(s) 2441
SspI AATATT 1 cut(s) 785
SstI GAGCTC 2 cut(s) 569, 4006
StyD4I CCNGG 6 cut(s) 38, 2147, 2705, 2728, 3004, 3170
StyI CCWWGG 6 cut(s) 131, 279, 445, 744, 875, 954
TaaI ACNGT 6 cut(s) 212, 1635, 3232, 3342, 3577, 3667
TaiI ACGT 4 cut(s) 72, 94, 1956, 2949
TatI WGTACW 3 cut(s) 726, 1172, 3737
TfiI GAWTC 9 cut(s) 1199, 1778, 1942, 2484, 2680, 3123, 3143, 3603, 4129
TseFI GTSAC 5 cut(s) 590, 1061, 2215, 2928, 3760
TseI GCWGC 7 cut(s) 268, 998, 2585, 2974, 3830, 3926, 3941
Tsp45I GTSAC 5 cut(s) 590, 1061, 2215, 2928, 3760
TspGWI ACGGA 4 cut(s) 716, 1038, 3075, 3370
Vha464I CTTAAG 1 cut(s) 3617
VneI GTGCAC 2 cut(s) 2227, 2685
VpaK11BI GGWCC 8 cut(s) 135, 580, 879, 1205, 1766, 3187, 3370, 4029
XagI CCTNNNNNAGG 1 cut(s) 3003
XapI RAATTY 9 cut(s) 300, 540, 992, 1237, 1257, 1642, 1802, 1964, 2771
XceI RCATGY 6 cut(s) 293, 1277, 1536, 1822, 1951, 2305
XcmI CCANNNNNNNNNTGG 2 cut(s) 1965, 3178
XhoI CTCGAG 1 cut(s) 2820
XmiI GTMKAC 2 cut(s) 750, 1138
XmnI GAANNNNTTC 4 cut(s) 116, 2379, 2679, 2957
ZrmI AGTACT 1 cut(s) 1174
Zsp2I ATGCAT 5 cut(s) 235, 721, 1534, 3465, 3651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.