Rw5G038280

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000071
Physical Location & Seq
Reverse (-)
67528413 .. 67532848
4436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038280.1

Sequence Viewer

Length: 3141 bp
ATGGCAGGTGATGAAGATCAGAAGGTCATTCAAATTGGCACGTCTTCGAATTCACTGAAGAACATAGAACCATGGGAAAACTCAAACCATCCGCTTTTCCTCCATCATTCAGATCAGCCTGGCGCGGTACTCGTATCACAGCCTTTGGTGGAAGACAACTACACAACTTGGATTCAATCTATGAGCATGGCTCTTACAATCAAGAACAAGAAGGGATTCGTTGATGGATCCATCAAGAGGCCAACTGATAGGGTTCAAGAACAACAACAGTGGGATCGATGCAACATACTGGTGAAAACGTGGCTGCTGGGATCCATGTCAAAAGAAATTTCTGGCAGTGTTATTCACTGCAAGGATGCACGAAGCATGTGGCTTGAATTGCAAGAACGGTTCTCGCATACAAACACTGTGCAACTATTTCATATAGAAAACGCCATTCATGATTGTGAACAAGGCAGTACCTCAGTAACATCTTTCTTTACCAAGCTCAAAACTCTTTGGGATGAGAGAGACGTCCTTTGTGAAATCCCTCCATGTAGTTGTGAGACAGCTAAGGAGATCAAGGATTACATGGAAACTCAGAAGACAATGAAATTTTTGATGGGTCTCAATGAAAATTATGCCACGATTCGAAGCAATATCGTGGGAATGGATCCTCTTCCAAGCGTGAATAAAGCGTATTCCATGGCACTCCGACATGAGAAGCAAGCAGAGGTGTCAAGCGGAAAGGCATTGATTCAGCCGGAAGCTGCTGCCTTTGTTGTGAAGAAAGCGAACCGTGAGTTTGAAATTGGAGAAGGTGAAGCTAAATGGCATACTTACGAGTACTGTCGCAAAAGAAAGGCTGCTATGGAGGGAGGACAAGTACGTGCGAAAGGAAATCACGTTGTTTGCATGAATGATAAAAGAGAAGCTGCTCCTAGTTTCCCATTTTCACAGGAAGAGTGCCAGCAGCTCATGAGTCTCCTAAACAAGAACAAAACTGCCACAGCAAATCAAGTTGGTAATGTTCCAGACTTTGATGAACTCTCAGGTAAAGCGTTTACTCTCTCTCAAGATGGCAAAGAAACAATATGGATATTGGATAGTGGGGCTAGTGATCACATAGTTTATGTCTTTCCATTTCAATCCCACACAAATTCGAATTCTCTATCTCCTCTGCCTCTTTTTCCACAACACTTACAATCTCCATATCTTGATTCTGACCTTCCAATTTCGTCTCCACCACAGCATGAAAACTCAATCAATGAAAATCCTCATCATGATCTTTCTTCCACTGCAAGTCAATCCCATTCTCCTATCACCAATCCCTCTTCATCATCAGACTTAATTATTCCTCCATCTACAGATCACTCTTCTTCTCCTAATTCACATACTGAGCCTACCACCATGACACCTTCACCACCATCACCAATACGTCGGTCCTCCAGACCTACCAAAACTCCATCATATTTGCAGGATTTCCATGTCGAGGCCTCCCTCCCCTCCCGACTCACACCGTCATCTTCAACGAACGTGGTTCTTCCCTCAGGTACTGCTCACCCTCTTTCTCATGTCTTATCTTATGATAGACTTTCTTCTCATCACCGAGTCTTCACCACTAAACTCACAATCCACCGAGAACCAACTTCTTTTTCCCAGGCCGTTCAGAGTCCACAATGGAGAGAGGCAATGCACAAGGAGATTCAAGCACTCCAAGCAAACAATACTTGGAGTTTGGTGCCCTTACCACCTCATAAACGACCCATTGGTTGCAAATGGGTTTACAAGATCAAACTCAATCCAGATGGCACTATTGAGAGATACAAGGCACGCCTAGTTGCTAAGGGATACAGTCAGATTGAAGGGGTTGACTACCGTGAAACTTTCGCCCCGGTAGCCAAACTAACAACAGTGAGAGTTCTTCTTAGTCTTGCCGCTATGCAAGGTTGGCATTTACACCAATTGGACGTGAACAATGCGTTTCTTCATGGTGATCTATATGAGGAAGTCTACATGCATATACCTCCTGGGTTCGGACGAAAGGGGGAGACTAGGGTTTGCAAGCTACACAAGTCACTTTACGGGTTAAAACAAGCTTCACGTCAATGGTTCATCAAGCTTTCAACAGCTCTCAAGTCTGCAGGGTTCCATCAATCTTGGTCTGATTATTCACTATTCGTCCGAACTCATCAGGGAAAATTCACAGCTTTATTGGTTTATGTTGACGATGTAATCTTGGCAGGAAACAGTTTGAAAGATATCATGGACACCAAGCAATTTCTTTCCAGTCATTTCAAGCTAAAGGATATGGGGCAGCTAAAGTATTTTTTGGGAATAGAAGTGGCCAGATCAAAGCAAGGCATTGCAATATGTCAGAGGAAGTATGCCCTAGAGATTCTTGAAGACACAGGTTTTCTTGGTGCCAAGTCCTCACGTTTCCCTGTTGATCAAAGTATATCACTCACACAAACAAATGGAGATTTGCTAGATGATCCATCACAATATCGCAGATTGGTAGGGCGGCTTATATACCTTACTATTACAAGGCCTGATCTTGTTTATGCTGTCCACTTACTAAGTCAGTTTATGGACAAGCCCAGGCAACCACATCTTGAGGCAGCGCATAAGGTCTTAAGGTACATTAAACAAACACCAGGGCAAGGGATTCTTTTGCCTTCCACAGGGTCATTGGACCTAAGGGCCTATTGCGACGCTGACTGGGCTCGTTATAAGGACACAAGGAGGTCAGTGTCAGGATACTGCATTCTTTTTGGTAATGCACCAGTTTCATGGAAAACAAAGAAGCAGAGTACAGTAGCTGTTCAAGTGACTTGGTTGCGCAATATATTGAAGGATTTGAATGTAAATCATACACAACCAGTTCGGTTATTTTGCGACAACCAAGCTGCTATTCATATAGCATCAAATCCAGTATTTCATGAGCGCACAAAGCATATAGAAATCGATTGTCATGTTGTTCGTGAAAAGGTGCAAAGAGGAATAGTTAAAACAACATACGTTCGCACCAAAGAGCAACCAGCAGACCTGTTTACCAAACCCCTGAGTTCAATCCAGTTTGAGGCATTACTTCGCAAGTTGAGTGTAATCAACATACACTCCAACTTGAGGGGGAGTATTGAAGGAACGAATCCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

1046

Amino Acids

117.97

Weight (kDa)

8.89

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 35 - 81 2e-17 gag-polypeptide of LTR copia-type
RVT_2 PF07727 568 - 809 1e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2712
AatII GACGTC 1 cut(s) 516
Acc16I TGCGCA 1 cut(s) 2822
AccB1I GGYRCC 2 cut(s) 1720, 2402
AccI GTMKAC 1 cut(s) 1992
AccII CGCG 1 cut(s) 125
AciI CCGC 5 cut(s) 92, 125, 723, 1917, 2503
AclWI GGATC 8 cut(s) 222, 235, 282, 306, 319, 647, 660, 2468
AcoI YGGCCR 1 cut(s) 2325
AcsI RAATTY 6 cut(s) 49, 327, 593, 1138, 1144, 2180
AcuI CTGAAG 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 7 cut(s) 129, 460, 827, 867, 1534, 2621, 2794
AfiI CCNNNNNNNGG 7 cut(s) 237, 1471, 2015, 2642, 2663, 3061, 3108
AflII CTTAAG 1 cut(s) 2614
AjiI CACGTC 3 cut(s) 42, 1951, 2084
AjnI CCWGG 5 cut(s) 118, 1638, 2008, 2579, 2635
AjuI GAANNNNNNNTTGG 4 cut(s) 1618, 1650, 2877, 2909
Alw26I GTCTC 6 cut(s) 504, 539, 611, 968, 1224, 2024
AlwI GGATC 8 cut(s) 222, 235, 282, 306, 319, 647, 660, 2468
AlwNI CAGNNNCTG 2 cut(s) 1535, 2801
AoxI GGCC 6 cut(s) 239, 1473, 1641, 2325, 2528, 2682
ApeKI GCWGC 9 cut(s) 304, 749, 752, 845, 914, 952, 2296, 2600, 2888
ApoI RAATTY 6 cut(s) 49, 327, 593, 1138, 1144, 2180
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 4 cut(s) 125, 2605, 2823, 2928
AspS9I GGNCC 3 cut(s) 1422, 2674, 2682
AsuC2I CCSGG 1 cut(s) 1874
AsuII TTCGAA 3 cut(s) 47, 631, 1142
AvaII GGWCC 2 cut(s) 1422, 2674
AxyI CCTNAGG 2 cut(s) 1528, 2678
BaeGI GKGCMC 1 cut(s) 1725
BalI TGGCCA 1 cut(s) 2327
BamHI GGATCC 3 cut(s) 227, 311, 652
BanI GGYRCC 2 cut(s) 1720, 2402
BanII GRGCYC 1 cut(s) 2707
BbsI GAAGAC 5 cut(s) 36, 159, 590, 1585, 2391
BbvI GCAGC 9 cut(s) 291, 736, 739, 832, 901, 964, 2308, 2612, 2875
BceAI ACGGC 1 cut(s) 1628
BciT130I CCWGG 5 cut(s) 120, 1640, 2010, 2581, 2637
BciVI GTATCC 2 cut(s) 1823, 2732
BclI TGATCA 2 cut(s) 1099, 2428
BcnI CCSGG 1 cut(s) 1874
BcoDI GTCTC 6 cut(s) 504, 539, 611, 968, 1224, 2024
BfaI CTAG 6 cut(s) 921, 1095, 1817, 2034, 2372, 2468
BfmI CTRYAG 2 cut(s) 1344, 2121
BfrI CTTAAG 1 cut(s) 2614
BfuI GTATCC 2 cut(s) 1823, 2732
BmcAI AGTACT 1 cut(s) 827
Bme1390I CCNGG 6 cut(s) 120, 1640, 1874, 2010, 2581, 2637
Bme18I GGWCC 2 cut(s) 1422, 2674
BmgBI CACGTC 3 cut(s) 42, 1951, 2084
BmgT120I GGNCC 3 cut(s) 1422, 2674, 2682
BmiI GGNNCC 6 cut(s) 229, 313, 654, 1722, 2129, 2404
BmrFI CCNGG 6 cut(s) 120, 1640, 1874, 2010, 2581, 2637
BmrI ACTGGG 1 cut(s) 2710
BmsI GCATC 3 cut(s) 269, 346, 2912
BmuI ACTGGG 1 cut(s) 2710
BpiI GAAGAC 5 cut(s) 36, 159, 590, 1585, 2391
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 1411
Bpu10I CCTNAGC 2 cut(s) 552, 1824
Bpu14I TTCGAA 3 cut(s) 47, 631, 1142
BpuEI CTTGAG 4 cut(s) 1038, 2099, 2615, 3127
BpuMI CCSGG 1 cut(s) 1874
Bsa29I ATCGAT 2 cut(s) 277, 2946
BsaAI YACGTR 1 cut(s) 869
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 7 cut(s) 71, 684, 1638, 1872, 2009, 2579, 2636
BsaXI ACNNNNNCTCC 6 cut(s) 849, 879, 1426, 1456, 3083, 3113
Bsc4I CCNNNNNNNGG 7 cut(s) 237, 1471, 2015, 2642, 2663, 3061, 3108
Bse1I ACTGG 7 cut(s) 294, 2268, 2705, 2765, 2861, 2912, 3055
Bse21I CCTNAGG 2 cut(s) 1528, 2678
Bse3DI GCAATG 2 cut(s) 1677, 2343
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 5 cut(s) 120, 1640, 2010, 2581, 2637
BseCI ATCGAT 2 cut(s) 277, 2946
BseDI CCNNGG 7 cut(s) 71, 684, 1638, 1872, 2009, 2579, 2636
BseGI GGATG 3 cut(s) 88, 361, 508
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 7 cut(s) 237, 1471, 2015, 2642, 2663, 3061, 3108
BseMI GCAATG 2 cut(s) 1677, 2343
BseMII CTCAG 6 cut(s) 477, 593, 1044, 1368, 1542, 3035
BseNI ACTGG 7 cut(s) 294, 2268, 2705, 2765, 2861, 2912, 3055
BseRI GAGGAG 1 cut(s) 1146
BseSI GKGCMC 1 cut(s) 1725
BseXI GCAGC 9 cut(s) 291, 736, 739, 832, 901, 964, 2308, 2612, 2875
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 6 cut(s) 241, 1475, 1643, 2327, 2530, 2684
BshNI GGYRCC 2 cut(s) 1720, 2402
BshVI ATCGAT 2 cut(s) 277, 2946
BsiSI CCGG 2 cut(s) 743, 1874
BslI CCNNNNNNNGG 7 cut(s) 237, 1471, 2015, 2642, 2663, 3061, 3108
BsmAI GTCTC 6 cut(s) 504, 539, 611, 968, 1224, 2024
BsmBI CGTCTC 2 cut(s) 504, 1224
BsmI GAATGC 1 cut(s) 2745
BsnI GGCC 6 cut(s) 241, 1475, 1643, 2327, 2530, 2684
Bso31I GGTCTC 1 cut(s) 611
Bsp119I TTCGAA 3 cut(s) 47, 631, 1142
Bsp1286I GDGCHC 2 cut(s) 1725, 2707
Bsp19I CCATGG 2 cut(s) 71, 684
BspACI CCGC 5 cut(s) 92, 125, 723, 1917, 2503
BspANI GGCC 6 cut(s) 241, 1475, 1643, 2327, 2530, 2684
BspCNI CTCAG 6 cut(s) 476, 592, 1043, 1369, 1541, 3036
BspDI ATCGAT 2 cut(s) 277, 2946
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 4 cut(s) 439, 957, 1261, 2920
BspLI GGNNCC 6 cut(s) 229, 313, 654, 1722, 2129, 2404
BspMAI CTGCAG 1 cut(s) 2125
BspPI GGATC 8 cut(s) 222, 235, 282, 306, 319, 647, 660, 2468
BspT104I TTCGAA 3 cut(s) 47, 631, 1142
BspT107I GGYRCC 2 cut(s) 1720, 2402
BspTI CTTAAG 1 cut(s) 2614
BspTNI GGTCTC 1 cut(s) 611
BsrDI GCAATG 2 cut(s) 1677, 2343
BsrI ACTGG 7 cut(s) 294, 2268, 2705, 2765, 2861, 2912, 3055
BssECI CCNNGG 7 cut(s) 71, 684, 1638, 1872, 2009, 2579, 2636
BssNI GRCGYC 1 cut(s) 513
BssT1I CCWWGG 2 cut(s) 71, 684
Bst2UI CCWGG 5 cut(s) 120, 1640, 2010, 2581, 2637
Bst6I CTCTTC 4 cut(s) 663, 936, 1318, 1360
BstACI GRCGYC 1 cut(s) 513
BstAFI CTTAAG 1 cut(s) 2614
BstBAI YACGTR 1 cut(s) 869
BstBI TTCGAA 3 cut(s) 47, 631, 1142
BstC8I GCNNGC 4 cut(s) 708, 950, 1813, 2045
BstDSI CCRYGG 2 cut(s) 71, 684
BstENI CCTNNNNNAGG 1 cut(s) 2661
BstF5I GGATG 3 cut(s) 88, 361, 508
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 4 cut(s) 125, 2605, 2823, 2928
BstMAI GTCTC 6 cut(s) 504, 539, 611, 968, 1224, 2024
BstMWI GCNNNNNNNGC 6 cut(s) 379, 1697, 1877, 1930, 2702, 2932
BstNI CCWGG 5 cut(s) 120, 1640, 2010, 2581, 2637
BstNSI RCATGY 2 cut(s) 370, 1999
BstSCI CCNGG 6 cut(s) 118, 1638, 1872, 2008, 2579, 2635
BstSFI CTRYAG 2 cut(s) 1344, 2121
BstSLI GKGCMC 1 cut(s) 1725
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 9 cut(s) 291, 736, 739, 832, 901, 964, 2308, 2612, 2875
BstV2I GAAGAC 5 cut(s) 36, 159, 590, 1585, 2391
BstX2I RGATCY 3 cut(s) 227, 311, 652
BstXI CCANNNNNNTGG 1 cut(s) 2772
BstYI RGATCY 3 cut(s) 227, 311, 652
Bsu15I ATCGAT 2 cut(s) 277, 2946
Bsu36I CCTNAGG 2 cut(s) 1528, 2678
BsuI GTATCC 2 cut(s) 1823, 2732
BsuRI GGCC 6 cut(s) 241, 1475, 1643, 2327, 2530, 2684
BsuTUI ATCGAT 2 cut(s) 277, 2946
BtgI CCRYGG 2 cut(s) 71, 684
BtrI CACGTC 3 cut(s) 42, 1951, 2084
BtsCI GGATG 3 cut(s) 88, 361, 508
BtsI GCAGTG 3 cut(s) 343, 346, 1275
BtsIMutI CAGTG 8 cut(s) 53, 275, 343, 346, 405, 1275, 1899, 2736
Cac8I GCNNGC 4 cut(s) 708, 950, 1813, 2045
CaiI CAGNNNCTG 2 cut(s) 1535, 2801
CciI TCATGA 4 cut(s) 439, 957, 1261, 2920
CfoI GCGC 4 cut(s) 125, 2605, 2823, 2928
Cfr13I GGNCC 3 cut(s) 1422, 2674, 2682
ClaI ATCGAT 2 cut(s) 277, 2946
CseI GACGC 1 cut(s) 2702
Csp6I GTAC 7 cut(s) 128, 459, 826, 866, 1533, 2620, 2793
CspCI CAANNNNNGTGG 4 cut(s) 251, 286, 1497, 1532
CviQI GTAC 7 cut(s) 128, 459, 826, 866, 1533, 2620, 2793
EaeI YGGCCR 1 cut(s) 2325
Eam1104I CTCTTC 4 cut(s) 663, 936, 1318, 1360
EarI CTCTTC 4 cut(s) 663, 936, 1318, 1360
Eco130I CCWWGG 2 cut(s) 71, 684
Eco147I AGGCCT 2 cut(s) 1475, 2530
Eco24I GRGCYC 1 cut(s) 2707
Eco31I GGTCTC 1 cut(s) 611
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 2 cut(s) 1422, 2674
Eco57I CTGAAG 1 cut(s) 77
Eco81I CCTNAGG 2 cut(s) 1528, 2678
EcoNI CCTNNNNNAGG 1 cut(s) 2661
EcoO109I RGGNCCY 1 cut(s) 2682
EcoRI GAATTC 2 cut(s) 49, 1144
EcoRII CCWGG 5 cut(s) 118, 1638, 2008, 2579, 2635
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 2 cut(s) 71, 684
EcoT22I ATGCAT 1 cut(s) 2001
EcoT38I GRGCYC 1 cut(s) 2707
ErhI CCWWGG 2 cut(s) 71, 684
Esp3I CGTCTC 2 cut(s) 504, 1224
FalI AAGNNNNNCTT 2 cut(s) 2634, 2666
FbaI TGATCA 2 cut(s) 1099, 2428
FblI GTMKAC 1 cut(s) 1992
FokI GGATG 3 cut(s) 75, 368, 515
FriOI GRGCYC 1 cut(s) 2707
FspBI CTAG 6 cut(s) 921, 1095, 1817, 2034, 2372, 2468
FspI TGCGCA 1 cut(s) 2822
GlaI GCGC 4 cut(s) 124, 2604, 2822, 2927
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 1411
HaeIII GGCC 6 cut(s) 241, 1475, 1643, 2327, 2530, 2684
HapII CCGG 2 cut(s) 743, 1874
HgaI GACGC 1 cut(s) 2702
HhaI GCGC 4 cut(s) 125, 2605, 2823, 2928
Hin1I GRCGYC 1 cut(s) 513
Hin6I GCGC 4 cut(s) 123, 2603, 2821, 2926
HinP1I GCGC 4 cut(s) 123, 2603, 2821, 2926
HincII GTYRAC 2 cut(s) 1852, 2206
HindII GTYRAC 2 cut(s) 1852, 2206
HindIII AAGCTT 2 cut(s) 2076, 2099
HpaII CCGG 2 cut(s) 743, 1874
Hpy99I CGWCG 2 cut(s) 1422, 2696
HpyAV CCTTC 9 cut(s) 16, 205, 791, 1217, 1407, 1838, 2667, 2827, 3116
HpyF10VI GCNNNNNNNGC 6 cut(s) 379, 1697, 1877, 1930, 2702, 2932
Hsp92I GRCGYC 1 cut(s) 513
HspAI GCGC 4 cut(s) 123, 2603, 2821, 2926
Ksp22I TGATCA 2 cut(s) 1099, 2428
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 9 cut(s) 291, 736, 739, 832, 901, 964, 2308, 2612, 2875
LweI GCATC 3 cut(s) 269, 346, 2912
MaeI CTAG 6 cut(s) 921, 1095, 1817, 2034, 2372, 2468
MaeIII GTNAC 3 cut(s) 466, 2055, 2809
MfeI CAATTG 1 cut(s) 1943
MflI RGATCY 3 cut(s) 227, 311, 652
MhlI GDGCHC 2 cut(s) 1725, 2707
MlsI TGGCCA 1 cut(s) 2327
MluNI TGGCCA 1 cut(s) 2327
MlyI GAGTC 4 cut(s) 970, 1485, 1599, 1660
MmeI TCCRAC 2 cut(s) 718, 3126
Mox20I TGGCCA 1 cut(s) 2327
Mph1103I ATGCAT 1 cut(s) 2001
MroXI GAANNNNTTC 1 cut(s) 215
MscI TGGCCA 1 cut(s) 2327
MseI TTAA 5 cut(s) 1328, 2069, 2615, 2625, 2988
MslI CAYNNNNRTG 3 cut(s) 290, 444, 2086
Msp20I TGGCCA 1 cut(s) 2327
MspCI CTTAAG 1 cut(s) 2614
MspI CCGG 2 cut(s) 743, 1874
MspR9I CCNGG 6 cut(s) 120, 1640, 1874, 2010, 2581, 2637
MunI CAATTG 1 cut(s) 1943
Mva1269I GAATGC 1 cut(s) 2745
MvaI CCWGG 5 cut(s) 120, 1640, 2010, 2581, 2637
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 6 cut(s) 379, 1697, 1877, 1930, 2702, 2932
NciI CCSGG 1 cut(s) 1874
NcoI CCATGG 2 cut(s) 71, 684
NlaIV GGNNCC 6 cut(s) 229, 313, 654, 1722, 2129, 2404
NmuCI GTSAC 2 cut(s) 2055, 2809
NsbI TGCGCA 1 cut(s) 2822
NsiI ATGCAT 1 cut(s) 2001
NspI RCATGY 2 cut(s) 370, 1999
NspV TTCGAA 3 cut(s) 47, 631, 1142
PagI TCATGA 4 cut(s) 439, 957, 1261, 2920
PceI AGGCCT 2 cut(s) 1475, 2530
PctI GAATGC 1 cut(s) 2745
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 9 cut(s) 172, 216, 628, 736, 1199, 1684, 2377, 2647, 3130
PleI GAGTC 4 cut(s) 969, 1485, 1598, 1659
PpsI GAGTC 4 cut(s) 969, 1485, 1598, 1659
Ppu21I YACGTR 1 cut(s) 869
PsiI TTATAA 1 cut(s) 2712
Psp6I CCWGG 5 cut(s) 118, 1638, 2008, 2579, 2635
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 5 cut(s) 118, 1638, 2008, 2579, 2635
PspN4I GGNNCC 6 cut(s) 229, 313, 654, 1722, 2129, 2404
PspPI GGNCC 3 cut(s) 1422, 2674, 2682
PstI CTGCAG 1 cut(s) 2125
PstNI CAGNNNCTG 2 cut(s) 1535, 2801
PsuI RGATCY 3 cut(s) 227, 311, 652
RsaI GTAC 7 cut(s) 129, 460, 827, 867, 1534, 2621, 2794
RsaNI GTAC 7 cut(s) 128, 459, 826, 866, 1533, 2620, 2793
RseI CAYNNNNRTG 3 cut(s) 290, 444, 2086
SaqAI TTAA 5 cut(s) 1328, 2069, 2615, 2625, 2988
Sau96I GGNCC 3 cut(s) 1422, 2674, 2682
ScaI AGTACT 1 cut(s) 827
SchI GAGTC 4 cut(s) 970, 1485, 1599, 1660
ScrFI CCNGG 6 cut(s) 120, 1640, 1874, 2010, 2581, 2637
SduI GDGCHC 2 cut(s) 1725, 2707
SfaNI GCATC 3 cut(s) 269, 346, 2912
SfcI CTRYAG 2 cut(s) 1344, 2121
SfuI TTCGAA 3 cut(s) 47, 631, 1142
SinI GGWCC 2 cut(s) 1422, 2674
SmiMI CAYNNNNRTG 3 cut(s) 290, 444, 2086
SmlI CTYRAG 5 cut(s) 1053, 2114, 2594, 2614, 3106
SmoI CTYRAG 5 cut(s) 1053, 2114, 2594, 2614, 3106
SseBI AGGCCT 2 cut(s) 1475, 2530
SsiI CCGC 5 cut(s) 92, 125, 723, 1917, 2503
SspMI CTAG 6 cut(s) 921, 1095, 1817, 2034, 2372, 2468
StuI AGGCCT 2 cut(s) 1475, 2530
StyD4I CCNGG 6 cut(s) 118, 1638, 1872, 2008, 2579, 2635
StyI CCWWGG 2 cut(s) 71, 684
TaqI TCGA 6 cut(s) 47, 277, 631, 1142, 1470, 2946
TaqII GACCGA 1 cut(s) 1410
TatI WGTACW 2 cut(s) 825, 2792
TauI GCSGC 2 cut(s) 1919, 2506
TfiI GAWTC 9 cut(s) 172, 216, 628, 736, 1199, 1684, 2377, 2647, 3130
Tru1I TTAA 5 cut(s) 1328, 2069, 2615, 2625, 2988
Tru9I TTAA 5 cut(s) 1328, 2069, 2615, 2625, 2988
TscAI CASTG 8 cut(s) 60, 275, 343, 353, 412, 1282, 1899, 2736
TseFI GTSAC 2 cut(s) 2055, 2809
TseI GCWGC 9 cut(s) 304, 749, 752, 845, 914, 952, 2296, 2600, 2888
Tsp45I GTSAC 2 cut(s) 2055, 2809
TspRI CASTG 8 cut(s) 60, 275, 343, 353, 412, 1282, 1899, 2736
Vha464I CTTAAG 1 cut(s) 2614
VpaK11BI GGWCC 2 cut(s) 1422, 2674
XagI CCTNNNNNAGG 1 cut(s) 2661
XapI RAATTY 6 cut(s) 49, 327, 593, 1138, 1144, 2180
XceI RCATGY 2 cut(s) 370, 1999
XcmI CCANNNNNNNNNTGG 1 cut(s) 2668
XmiI GTMKAC 1 cut(s) 1992
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 6 cut(s) 921, 1095, 1817, 2034, 2372, 2468
ZraI GACGTC 1 cut(s) 514
ZrmI AGTACT 1 cut(s) 827
Zsp2I ATGCAT 1 cut(s) 2001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.