MD10G1196700.v1.1

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000071
Physical Location & Seq
Forward (+)
29461628 .. 29465810
4183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1196700.v1.1.491

Sequence Viewer

Length: 3777 bp
ATGGCCTTAACGGTCAAGAACAAACTTGGTTTTGTTGATGGAACGATCAACAAACCAAGTGAGGATAATTTTGAGGAGCTGCAGCAATGGAATCGCTGCAACAACTTGGTCAAGACATGGCTACTAGGCTCCATGTCAAAGGAGATTTCAGGGAGTGTCATCAACTACAAGGATGCTCGACAAATGTGGACCGATCTGCAGGAGAGGTTCTCACATGTGAATATAGTTCAGCTGTTCCATGTTGAGAACGAGATCCATGATTGCGTCCAAAGCAATATGAGTGTAAGTTCTTACTTCACTAAACTTAAAAGTTTATGGGATGAACGTGATACTTTGTGTTCCATTCCAGCATGCAGTTGTGGAACAAAGAATGAGATGAACTCATATGTTGAAACTCAGAAAACCATGAAGTTCCTTATGGGACTGAACGAATCGTATGCTACGGTTCGAAGCAATACTCTTCTTCTCGAACCACTGCTTACGATGAACAAGGCATATGCGTTGGTCATTCGACATGAACGCCAGGCAGAGGTTTCCAATGGGAAAAGCACACAACTAGAAACTGCTGTCTTTGCAGTGAAGAATCTGTCGCGAGAACCTACACCTGAAGACAAGGAGATGCGATGTCACATCACCAAAAATTGTCGTGCACATCTCAAGTGCACTTTTTACGGATGGAAAGGCCACACCTTCGATTTCTGTCGAAAACGGAAAGCAGCCACAGAGACCGAATCCAATCGTCTCTTTTCTTCAAAGGGGAATCAAGTTTCACAAAGTAACAAGCAAGAGACAGTGCCTAATTTCCCGTTCTCTCAGGAAGACTGCAAGCAAATCCTTCAGATGTTGAACAAGAACAACTCATCATTTGCCAATCAAGTCAATAATCTTCCTAGTCATGAAGAACTTTCAGGTAAAGCCTTCTCTTTTTCATGTAAAGGAACCAAAAGTATTTGGATCCTGGACAGTGGTGCCACGGATCACATAGTTTGCAGTCCTAGTCTCTTTACACACTCACGAGCTGTTGAAAATCATACAGTTGAACTGCCAAATGGTTCCGTTGCCAAAGTCACTCACATTGGACAAGTTGTTTTCTCTCATAATCTTATCCTTGAAAATGTCCTATGTTTGCTTTCTATTTCAGGTGCCAAGTATTTCCTTACCATCGTAGATGACTACACTAGAAGCACTTGGGTTTATTTGATGAAACATAAATCTGACACAAAAGACATTTTAGTCGGATTCATTCAAATGATTGAGACTCAATTCAATACCAGAGTTCAAATCATACGAAGTGACAATGGCCTTGAGTTTAAGCTTGAGCAATTTTATGCTCTCAAAGGCATCATACATCAATCTAGTTGTGTCAACACACCACAACAAAATGGTGTTGCCGAACGTAAACACCGGCATTTGCTCAACGTTGCTCGGGCTTTGCTCTTTCAAGCTTGTTTGCCAAAACGTTTTTGGGGGGATGCAATCTTAACCTCAGCCTACCTCATCAATAGAACACCCACTCCACTTCTCCAAGGTAAAACACCTTACGAAAAACTGTTTCACAAAGCACCTAACTATTCCCATTTAAAGGTTTTCGGTAGTTTATGTTTTTCTTCTACACACGCCCAAAGGCCCTCTAAATTCGACCCCTGTGCAATCCAATGCATTTTTCTTGGGTATCCTTATGGTCAAAAGGGTTATAAATTGTTTGATATAAAACACAATAAGACTTTTGTTTCTAGGGATGTTGTTTTTTTTGAAGATCATTTTCCATTCCAAAATCATACCACTTCCGCTGCCCCGTCTCCATCAAACATTACACCCTCACCCATCACGTTTGACTTGACCTCTCCCATTGAGTCGCACTCAAGCCTTGCACCCGAAGAAATAAACATCACCTCTCCCACACTTGCCAACATCACACCACTCTCATCTCCTTTACCTACTACAACTCCACCACCAGATACCTTATCAACACCTTCACATACCCTACACTCTTCACCACCTCCAACAACTCCATCACCAGACCAACCACCTCCACTCCGTCGTAGCACTCGACCGACCAAAACTTCCACATTTTTGCAGGACTTTCATGTTGAGGTGGCCCTCCCTTCCCGTGTTGCTCCCACATCTTCCACAAGCATGGTCCAGTCATCAGGTACATCTCACCCCATATCTCGTTATTTATCTTATGACCATGTATCCCCCACTCACAAAACATTTCTCACCACCCTCACCATTATCAAAGAACCCACCAGTTTTTCGCAAGCTGTTCAGGATCCAAAATGGCGTGACGCCATGCAAAAAGAGATTGTTGCCCTTCATGACAATCGCACTTGGACTCTTGTGCCCTTACCACCTCACAAACATCCCATCGGTTGCAAATGGATCTATAAGGTGAAGCTTAAACCTGACGGCAGTGTTGAACGCTACAAAGCTCGTCTCGTTGCTAAAGGCTACAGTCAAATTGAGGGGCTTGATTATCGGGAAACTTTTGCTCCAGTTGCCAAGCTCACCACCGTCCGCGTCCTCCCGAGTATTGCCTCCATACGAGGCTGGCATCTTCATCAACTTGATGTGAACAACGCATTTTTGCATGGTGACTTATACGAGGAGGTTTATATGTCCTTGCCTCCTGGGTTCGGACGAAAGGGGGAGACACGAGTATGCAAATTACACAAATCCATTTATGGTTTAAAACAGGCCTCGAGACAATGGTTTATCAAGTTGTCTAGTGCTCTCAAGACTGCTGGTTTTCATCAATCATGGTCAGATTACTCATTGTTCGTCCGAAACCGTCAAGGCAGTTTTATGGCATTACTTGTGTATGTTGATGACGTGATACTTGCAGGTAACAATTTGAGAGAAATTGAAGAGACTAAACTCTTCCTTTCCCAACATTTTAAACTAAAGGATTTGGGAAAACTCAAATACTTTTTGGGGATAGAAGTTGCAAGATCTAAACAAGGGATTGCCTTGTGCCAACGCAAATATGCGTTGGAGATACTAGAAGATGCTGGGTTCCTTGGTGCGAAGCCCTCACGATTACCAATGGAACCAAACCTGTCCCTTACACAAACTGATGGGACGCTCCTACATGATCCTTCTTCATACAAGCGACTCGTAGGGAGGCTGATTTACTTAACCATCACGCGGCCTGACCTGACTTATGTGGTTAATATGTTGAGCCAATTCATGGACAAGCCACGACAACCACACCTTGAAGCGGTGCATAAGGTTCTCAGGTACATCAAACAATCTCCTGGACAGGGAATTCTTTTGCCTTCTACAGGTTCACTACAATTACAAGCCTTTTGTGATGCTGATTGGGCTCGGTGTAAAGACACTAGAAGGTCAATAACCGGTTATTGTATCTTCCTTGGACAAGCACCGATATCTTGGAAAACGAAGAAACAAAGCACAATATCTCGTTCTAGTGCAGAGGCAGAATACCGTTCTATGGCTACTACTTGTTGTGAAGTTATATGGCTAAGAAACATTTTGAAGGATTTGCAAGTAAATCATGTACAACCAGTCACAATGTTTTGTGATAATCAAGCTGCCATGCACATTGCTTCGAACCCAGTGTTTCATGAACGAACGAAGCATATAGAGATAGACTGTCACCTAGTACGTGAGAAGATTCAAATGGGAATGGTGCAAACAGCTTACATACGGACCTCGAATCAACCTGCAGACTTGTTCACCAAACCACTAAGCTCGGCACAATTTGAGGTCTTAATTAGAAAGTTGGGTGTCATCGACATTCACACTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1259

Amino Acids

142.38

Weight (kDa)

9.18

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 2 - 107 3.6e-06 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 318 - 374 2.7e-08 Pol polyprotein, beta-barrel domain
rve PF00665 381 - 456 3.5e-08 Integrase core domain
SH3_retrovirus PF25597 532 - 594 1.6e-20 Retroviral polymerase SH3-like domain
RVT_2 PF07727 775 - 1017 2.2e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1695
AasI GACNNNNNNGTC 1 cut(s) 1232
Acc36I ACCTGC 2 cut(s) 2834, 3694
AccB1I GGYRCC 2 cut(s) 968, 1142
AccB7I CCANNNNNTGG 1 cut(s) 3190
AccII CGCG 3 cut(s) 592, 2520, 3148
AciI CCGC 4 cut(s) 1788, 2518, 3148, 3221
AclI AACGTT 2 cut(s) 1419, 1459
AclWI GGATC 8 cut(s) 247, 949, 962, 984, 2267, 2280, 2390, 3089
AcsI RAATTY 2 cut(s) 1634, 3267
AcuI CTGAAG 2 cut(s) 627, 821
AcyI GRCGYC 1 cut(s) 2289
AfaI GTAC 4 cut(s) 2155, 3242, 3522, 3627
AfiI CCNNNNNNNGG 9 cut(s) 529, 1582, 2636, 3147, 3190, 3220, 3263, 3284, 3454
AflIII ACRYGT 1 cut(s) 214
AgeI ACCGGT 1 cut(s) 3356
AjiI CACGTC 1 cut(s) 2833
AjnI CCWGG 4 cut(s) 522, 957, 2629, 3256
AjuI GAANNNNNNNTTGG 2 cut(s) 1647, 1679
AloI GAACNNNNNNTCC 2 cut(s) 1498, 1530
Alw21I GWGCWC 3 cut(s) 652, 665, 2734
Alw44I GTGCAC 2 cut(s) 648, 661
AlwI GGATC 8 cut(s) 247, 949, 962, 984, 2267, 2280, 2390, 3089
Ama87I CYCGRG 3 cut(s) 1425, 2527, 2701
AoxI GGCC 7 cut(s) 3, 682, 1300, 1625, 2097, 2697, 3149
ApaLI GTGCAC 2 cut(s) 648, 661
ApeKI GCWGC 6 cut(s) 79, 82, 96, 716, 1790, 3554
ApoI RAATTY 2 cut(s) 1634, 3267
AsiGI ACCGGT 1 cut(s) 3356
AspS9I GGNCC 5 cut(s) 189, 1626, 2098, 2140, 3672
AsuII TTCGAA 2 cut(s) 448, 3572
AvaI CYCGRG 3 cut(s) 1425, 2527, 2701
AvaII GGWCC 3 cut(s) 189, 2140, 3672
BaeGI GKGCMC 3 cut(s) 652, 665, 2346
BamHI GGATCC 2 cut(s) 954, 2272
BanI GGYRCC 2 cut(s) 968, 1142
BanII GRGCYC 1 cut(s) 3328
BauI CACGAG 2 cut(s) 1014, 2655
BbsI GAAGAC 2 cut(s) 615, 825
Bbv12I GWGCWC 3 cut(s) 652, 665, 2734
BbvCI CCTCAGC 1 cut(s) 1486
BbvI GCAGC 6 cut(s) 66, 83, 94, 728, 1777, 3541
BccI CCATC 9 cut(s) 32, 669, 1169, 1810, 1832, 2020, 2375, 3071, 3149
BceAI ACGGC 1 cut(s) 2425
BcgI CGANNNNNNTGC 6 cut(s) 74, 108, 419, 453, 1628, 1662
BciT130I CCWGG 4 cut(s) 524, 959, 2631, 3258
BciVI GTATCC 2 cut(s) 1683, 2206
BfmI CTRYAG 5 cut(s) 80, 197, 2452, 3282, 3687
BfuAI ACCTGC 2 cut(s) 2834, 3694
BfuI GTATCC 2 cut(s) 1683, 2206
BglII AGATCT 1 cut(s) 2951
BisI GCNGC 7 cut(s) 80, 83, 97, 717, 1791, 3149, 3555
BlsI GCNGC 7 cut(s) 81, 84, 98, 718, 1792, 3150, 3556
Bme1390I CCNGG 4 cut(s) 524, 959, 2631, 3258
Bme18I GGWCC 3 cut(s) 189, 2140, 3672
BmeT110I CYCGRG 3 cut(s) 1425, 2527, 2701
BmgBI CACGTC 1 cut(s) 2833
BmgT120I GGNCC 5 cut(s) 189, 1626, 2098, 2140, 3672
BmiI GGNNCC 9 cut(s) 130, 940, 956, 970, 1054, 1144, 2274, 3017, 3051
BmrFI CCNGG 4 cut(s) 524, 959, 2631, 3258
BmrI ACTGGG 1 cut(s) 3572
BmsI GCATC 7 cut(s) 163, 609, 1350, 1462, 2563, 2998, 3304
BmuI ACTGGG 1 cut(s) 3572
BpiI GAAGAC 2 cut(s) 615, 825
BplI GAGNNNNNCTC 6 cut(s) 194, 226, 365, 397, 1844, 1876
BpmI CTGGAG 1 cut(s) 2478
Bpu10I CCTNAGC 1 cut(s) 1486
Bpu14I TTCGAA 2 cut(s) 448, 3572
BpuEI CTTGAG 5 cut(s) 641, 1325, 1337, 1846, 2720
BsaAI YACGTR 1 cut(s) 3629
BsaHI GRCGYC 1 cut(s) 2289
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 5 cut(s) 972, 1525, 2630, 3019, 3373
BsaWI WCCGGW 1 cut(s) 3356
BsaXI ACNNNNNCTCC 8 cut(s) 1498, 1528, 1930, 1960, 2019, 2049, 2476, 2506
Bsc4I CCNNNNNNNGG 9 cut(s) 529, 1582, 2636, 3147, 3190, 3220, 3263, 3284, 3454
Bse118I RCCGGY 2 cut(s) 1404, 3356
Bse1I ACTGG 5 cut(s) 2143, 2250, 2495, 3527, 3578
Bse3DI GCAATG 2 cut(s) 92, 3564
BseBI CCWGG 4 cut(s) 524, 959, 2631, 3258
BseDI CCNNGG 5 cut(s) 972, 1525, 2630, 3019, 3373
BseGI GGATG 6 cut(s) 178, 325, 680, 1477, 1744, 2362
BseLI CCNNNNNNNGG 9 cut(s) 529, 1582, 2636, 3147, 3190, 3220, 3263, 3284, 3454
BseMI GCAATG 2 cut(s) 92, 3564
BseMII CTCAG 4 cut(s) 410, 827, 1500, 3250
BseNI ACTGG 5 cut(s) 2143, 2250, 2495, 3527, 3578
BseRI GAGGAG 2 cut(s) 89, 2621
BseSI GKGCMC 3 cut(s) 652, 665, 2346
BseXI GCAGC 6 cut(s) 66, 83, 94, 728, 1777, 3541
BseYI CCCAGC 1 cut(s) 3011
BsgI GTGCAG 1 cut(s) 3453
Bsh1236I CGCG 3 cut(s) 592, 2520, 3148
Bsh1285I CGRYCG 1 cut(s) 2054
BshFI GGCC 7 cut(s) 5, 684, 1302, 1627, 2099, 2699, 3151
BshNI GGYRCC 2 cut(s) 968, 1142
BshTI ACCGGT 1 cut(s) 3356
BsiEI CGRYCG 1 cut(s) 2054
BsiHKAI GWGCWC 3 cut(s) 652, 665, 2734
BsiHKCI CYCGRG 3 cut(s) 1425, 2527, 2701
BsiSI CCGG 2 cut(s) 1405, 3357
BslFI GGGAC 3 cut(s) 435, 3046, 3094
BslI CCNNNNNNNGG 9 cut(s) 529, 1582, 2636, 3147, 3190, 3220, 3263, 3284, 3454
BsmBI CGTCTC 3 cut(s) 746, 1803, 2441
BsmFI GGGAC 3 cut(s) 435, 3046, 3094
BsnI GGCC 7 cut(s) 5, 684, 1302, 1627, 2099, 2699, 3151
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 3 cut(s) 1425, 2527, 2701
Bsp119I TTCGAA 2 cut(s) 448, 3572
Bsp1286I GDGCHC 5 cut(s) 652, 665, 2346, 2734, 3328
Bsp1407I TGTACA 1 cut(s) 3520
Bsp68I TCGCGA 1 cut(s) 592
BspACI CCGC 4 cut(s) 1788, 2518, 3148, 3221
BspANI GGCC 7 cut(s) 5, 684, 1302, 1627, 2099, 2699, 3151
BspCNI CTCAG 4 cut(s) 409, 826, 1499, 3249
BspFNI CGCG 3 cut(s) 592, 2520, 3148
BspHI TCATGA 3 cut(s) 895, 2317, 3586
BspLI GGNNCC 9 cut(s) 130, 940, 956, 970, 1054, 1144, 2274, 3017, 3051
BspMAI CTGCAG 3 cut(s) 84, 201, 3691
BspMI ACCTGC 2 cut(s) 2834, 3694
BspPI GGATC 8 cut(s) 247, 949, 962, 984, 2267, 2280, 2390, 3089
BspT104I TTCGAA 2 cut(s) 448, 3572
BspT107I GGYRCC 2 cut(s) 968, 1142
BspTNI GGTCTC 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 92, 3564
BsrFI RCCGGY 2 cut(s) 1404, 3356
BsrGI TGTACA 1 cut(s) 3520
BsrI ACTGG 5 cut(s) 2143, 2250, 2495, 3527, 3578
BssAI RCCGGY 2 cut(s) 1404, 3356
BssECI CCNNGG 5 cut(s) 972, 1525, 2630, 3019, 3373
BssNI GRCGYC 1 cut(s) 2289
BssSI CACGAG 2 cut(s) 1014, 2655
BssT1I CCWWGG 3 cut(s) 1525, 3019, 3373
Bst2BI CACGAG 2 cut(s) 1014, 2655
Bst2UI CCWGG 4 cut(s) 524, 959, 2631, 3258
Bst6I CTCTTC 4 cut(s) 465, 1996, 2862, 2885
BstACI GRCGYC 1 cut(s) 2289
BstAUI TGTACA 1 cut(s) 3520
BstBAI YACGTR 1 cut(s) 3629
BstBI TTCGAA 2 cut(s) 448, 3572
BstC8I GCNNGC 4 cut(s) 352, 827, 2262, 2552
BstDEI CTNAG 6 cut(s) 396, 813, 1486, 3236, 3485, 3710
BstDSI CCRYGG 1 cut(s) 972
BstENI CCTNNNNNAGG 1 cut(s) 3282
BstF5I GGATG 6 cut(s) 178, 325, 680, 1477, 1744, 2362
BstFNI CGCG 3 cut(s) 592, 2520, 3148
BstMCI CGRYCG 1 cut(s) 2054
BstMWI GCNNNNNNNGC 4 cut(s) 270, 572, 2498, 3323
BstNI CCWGG 4 cut(s) 524, 959, 2631, 3258
BstNSI RCATGY 2 cut(s) 218, 354
BstSCI CCNGG 4 cut(s) 522, 957, 2629, 3256
BstSFI CTRYAG 5 cut(s) 80, 197, 2452, 3282, 3687
BstSLI GKGCMC 3 cut(s) 652, 665, 2346
BstUI CGCG 3 cut(s) 592, 2520, 3148
BstV1I GCAGC 6 cut(s) 66, 83, 94, 728, 1777, 3541
BstV2I GAAGAC 2 cut(s) 615, 825
BstX2I RGATCY 5 cut(s) 252, 954, 2272, 2382, 2951
BstXI CCANNNNNNTGG 1 cut(s) 2137
BstYI RGATCY 5 cut(s) 252, 954, 2272, 2382, 2951
BsuI GTATCC 2 cut(s) 1683, 2206
BsuRI GGCC 7 cut(s) 5, 684, 1302, 1627, 2099, 2699, 3151
BtgI CCRYGG 1 cut(s) 972
BtgZI GCGATG 1 cut(s) 637
BtrI CACGTC 1 cut(s) 2833
BtsCI GGATG 6 cut(s) 178, 325, 680, 1477, 1744, 2362
BtsI GCAGTG 3 cut(s) 473, 582, 2419
BtsIMutI CAGTG 6 cut(s) 473, 582, 798, 970, 2419, 3585
BtuMI TCGCGA 1 cut(s) 592
BveI ACCTGC 2 cut(s) 2834, 3694
Cac8I GCNNGC 4 cut(s) 352, 827, 2262, 2552
CciI TCATGA 3 cut(s) 895, 2317, 3586
Cfr10I RCCGGY 2 cut(s) 1404, 3356
Cfr13I GGNCC 5 cut(s) 189, 1626, 2098, 2140, 3672
CseI GACGC 4 cut(s) 253, 2297, 2509, 3091
Csp6I GTAC 4 cut(s) 2154, 3241, 3521, 3626
CspAI ACCGGT 1 cut(s) 3356
CviQI GTAC 4 cut(s) 2154, 3241, 3521, 3626
DdeI CTNAG 6 cut(s) 396, 813, 1486, 3236, 3485, 3710
DraI TTTAAA 3 cut(s) 1581, 2691, 2899
DrdI GACNNNNNNGTC 1 cut(s) 1232
DseDI GACNNNNNNGTC 1 cut(s) 1232
Eam1104I CTCTTC 4 cut(s) 465, 1996, 2862, 2885
EarI CTCTTC 4 cut(s) 465, 1996, 2862, 2885
Eco130I CCWWGG 3 cut(s) 1525, 3019, 3373
Eco147I AGGCCT 1 cut(s) 2699
Eco24I GRGCYC 1 cut(s) 3328
Eco31I GGTCTC 1 cut(s) 719
Eco32I GATATC 1 cut(s) 3390
Eco47I GGWCC 3 cut(s) 189, 2140, 3672
Eco57I CTGAAG 2 cut(s) 627, 821
Eco88I CYCGRG 3 cut(s) 1425, 2527, 2701
EcoNI CCTNNNNNAGG 1 cut(s) 3282
EcoO109I RGGNCCY 1 cut(s) 1626
EcoRI GAATTC 1 cut(s) 3267
EcoRII CCWGG 4 cut(s) 522, 957, 2629, 3256
EcoRV GATATC 1 cut(s) 3390
EcoT14I CCWWGG 3 cut(s) 1525, 3019, 3373
EcoT22I ATGCAT 1 cut(s) 1661
EcoT38I GRGCYC 1 cut(s) 3328
ErhI CCWWGG 3 cut(s) 1525, 3019, 3373
Esp3I CGTCTC 3 cut(s) 746, 1803, 2441
FaqI GGGAC 3 cut(s) 435, 3046, 3094
FauNDI CATATG 2 cut(s) 385, 496
Fnu4HI GCNGC 7 cut(s) 80, 83, 97, 717, 1791, 3149, 3555
FokI GGATG 6 cut(s) 185, 332, 687, 1484, 1751, 2349
FriOI GRGCYC 1 cut(s) 3328
Fsp4HI GCNGC 7 cut(s) 80, 83, 97, 717, 1791, 3149, 3555
GluI GCNGC 7 cut(s) 80, 83, 97, 717, 1791, 3149, 3555
GsaI CCCAGC 1 cut(s) 3015
GsuI CTGGAG 1 cut(s) 2478
HaeIII GGCC 7 cut(s) 5, 684, 1302, 1627, 2099, 2699, 3151
HapII CCGG 2 cut(s) 1405, 3357
HgaI GACGC 4 cut(s) 253, 2297, 2509, 3091
Hin1I GRCGYC 1 cut(s) 2289
HincII GTYRAC 1 cut(s) 1366
HindII GTYRAC 1 cut(s) 1366
HindIII AAGCTT 3 cut(s) 1313, 1443, 2396
HpaII CCGG 2 cut(s) 1405, 3357
Hpy166II GTNNAC 8 cut(s) 189, 650, 663, 1366, 1400, 2575, 3290, 3699
Hpy188I TCNGA 7 cut(s) 399, 840, 1216, 1238, 2639, 2767, 2786
Hpy8I GTNNAC 8 cut(s) 189, 650, 663, 1366, 1400, 2575, 3290, 3699
Hpy99I CGWCG 1 cut(s) 2043
HpyCH4IV ACGT 7 cut(s) 325, 1396, 1419, 1459, 1829, 2832, 3628
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 572, 2498, 3323
HpyF3I CTNAG 6 cut(s) 396, 813, 1486, 3236, 3485, 3710
HpySE526I ACGT 7 cut(s) 325, 1396, 1419, 1459, 1829, 2832, 3628
Hsp92I GRCGYC 1 cut(s) 2289
LmnI GCTCC 5 cut(s) 76, 134, 2122, 2497, 3090
Lsp1109I GCAGC 6 cut(s) 66, 83, 94, 728, 1777, 3541
LweI GCATC 7 cut(s) 163, 609, 1350, 1462, 2563, 2998, 3304
MaeII ACGT 7 cut(s) 325, 1396, 1419, 1459, 1829, 2832, 3628
MaeIII GTNAC 9 cut(s) 626, 776, 1066, 1292, 2285, 2594, 2846, 3529, 3617
MflI RGATCY 5 cut(s) 252, 954, 2272, 2382, 2951
MhlI GDGCHC 5 cut(s) 652, 665, 2346, 2734, 3328
MlyI GAGTC 4 cut(s) 1252, 1862, 2329, 3108
MmeI TCCRAC 3 cut(s) 1216, 2027, 2973
Mph1103I ATGCAT 1 cut(s) 1661
MslI CAYNNNNRTG 3 cut(s) 1247, 2135, 2659
MspA1I CMGCKG 2 cut(s) 232, 1790
MspI CCGG 2 cut(s) 1405, 3357
MspR9I CCNGG 4 cut(s) 524, 959, 2631, 3258
MvaI CCWGG 4 cut(s) 524, 959, 2631, 3258
MvnI CGCG 3 cut(s) 592, 2520, 3148
MwoI GCNNNNNNNGC 4 cut(s) 270, 572, 2498, 3323
NdeI CATATG 2 cut(s) 385, 496
NlaIV GGNNCC 9 cut(s) 130, 940, 956, 970, 1054, 1144, 2274, 3017, 3051
NmeAIII GCCGAG 1 cut(s) 3695
NmuCI GTSAC 7 cut(s) 626, 1066, 1292, 2285, 2594, 3529, 3617
NruI TCGCGA 1 cut(s) 592
NsiI ATGCAT 1 cut(s) 1661
NspI RCATGY 2 cut(s) 218, 354
NspV TTCGAA 2 cut(s) 448, 3572
PaeI GCATGC 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 2701
PagI TCATGA 3 cut(s) 895, 2317, 3586
PceI AGGCCT 1 cut(s) 2699
PciI ACATGT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 2047
PfeI GAWTC 8 cut(s) 91, 431, 583, 731, 760, 1239, 3637, 3679
PflMI CCANNNNNTGG 1 cut(s) 3190
PfoI TCCNGGA 2 cut(s) 957, 3256
PinAI ACCGGT 1 cut(s) 3356
PkrI GCNGC 7 cut(s) 81, 84, 98, 718, 1792, 3150, 3556
PleI GAGTC 4 cut(s) 1252, 1861, 2329, 3108
PpsI GAGTC 4 cut(s) 1252, 1861, 2329, 3108
Ppu21I YACGTR 1 cut(s) 3629
PscI ACATGT 1 cut(s) 214
PsiI TTATAA 1 cut(s) 1695
Psp1406I AACGTT 2 cut(s) 1419, 1459
Psp6I CCWGG 4 cut(s) 522, 957, 2629, 3256
PspFI CCCAGC 1 cut(s) 3011
PspGI CCWGG 4 cut(s) 522, 957, 2629, 3256
PspN4I GGNNCC 9 cut(s) 130, 940, 956, 970, 1054, 1144, 2274, 3017, 3051
PspPI GGNCC 5 cut(s) 189, 1626, 2098, 2140, 3672
PstI CTGCAG 3 cut(s) 84, 201, 3691
PsuI RGATCY 5 cut(s) 252, 954, 2272, 2382, 2951
PvuII CAGCTG 1 cut(s) 232
RruI TCGCGA 1 cut(s) 592
RsaI GTAC 4 cut(s) 2155, 3242, 3522, 3627
RsaNI GTAC 4 cut(s) 2154, 3241, 3521, 3626
RseI CAYNNNNRTG 3 cut(s) 1247, 2135, 2659
SatI GCNGC 7 cut(s) 80, 83, 97, 717, 1791, 3149, 3555
Sau96I GGNCC 5 cut(s) 189, 1626, 2098, 2140, 3672
SchI GAGTC 4 cut(s) 1252, 1862, 2329, 3108
ScrFI CCNGG 4 cut(s) 524, 959, 2631, 3258
SduI GDGCHC 5 cut(s) 652, 665, 2346, 2734, 3328
SfaNI GCATC 7 cut(s) 163, 609, 1350, 1462, 2563, 2998, 3304
SfcI CTRYAG 5 cut(s) 80, 197, 2452, 3282, 3687
Sfr274I CTCGAG 1 cut(s) 2701
SfuI TTCGAA 2 cut(s) 448, 3572
SinI GGWCC 3 cut(s) 189, 2140, 3672
SlaI CTCGAG 1 cut(s) 2701
SmiMI CAYNNNNRTG 3 cut(s) 1247, 2135, 2659
SmlI CTYRAG 6 cut(s) 656, 1304, 1316, 1861, 2701, 2735
SmoI CTYRAG 6 cut(s) 656, 1304, 1316, 1861, 2701, 2735
SphI GCATGC 1 cut(s) 354
SseBI AGGCCT 1 cut(s) 2699
SsiI CCGC 4 cut(s) 1788, 2518, 3148, 3221
StuI AGGCCT 1 cut(s) 2699
StyD4I CCNGG 4 cut(s) 522, 957, 2629, 3256
StyI CCWWGG 3 cut(s) 1525, 3019, 3373
TaiI ACGT 7 cut(s) 328, 1399, 1422, 1462, 1832, 2835, 3631
TaqII GACCGA 3 cut(s) 206, 743, 2068
TatI WGTACW 1 cut(s) 3520
TauI GCSGC 1 cut(s) 3151
TfiI GAWTC 8 cut(s) 91, 431, 583, 731, 760, 1239, 3637, 3679
TscAI CASTG 6 cut(s) 480, 582, 798, 970, 2419, 3585
TseFI GTSAC 7 cut(s) 626, 1066, 1292, 2285, 2594, 3529, 3617
TseI GCWGC 6 cut(s) 79, 82, 96, 716, 1790, 3554
Tsp45I GTSAC 7 cut(s) 626, 1066, 1292, 2285, 2594, 3529, 3617
TspGWI ACGGA 6 cut(s) 687, 724, 989, 1045, 2027, 3685
TspRI CASTG 6 cut(s) 480, 582, 798, 970, 2419, 3585
Van91I CCANNNNNTGG 1 cut(s) 3190
VneI GTGCAC 2 cut(s) 648, 661
VpaK11BI GGWCC 3 cut(s) 189, 2140, 3672
XagI CCTNNNNNAGG 1 cut(s) 3282
XapI RAATTY 2 cut(s) 1634, 3267
XceI RCATGY 2 cut(s) 218, 354
XcmI CCANNNNNNNNNTGG 1 cut(s) 1461
XhoI CTCGAG 1 cut(s) 2701
Zsp2I ATGCAT 1 cut(s) 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.